bsu36i  (New England Biolabs)


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  • 96
    Name:
    Bsu36I
    Description:
    Bsu36I 5 000 units
    Catalog Number:
    R0524L
    Price:
    269
    Category:
    Restriction Enzymes
    Size:
    5 000 units
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    Structured Review

    New England Biolabs bsu36i
    Bsu36I
    Bsu36I 5 000 units
    https://www.bioz.com/result/bsu36i/product/New England Biolabs
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bsu36i - by Bioz Stars, 2021-06
    96/100 stars

    Images

    1) Product Images from "Epigenetic CRBP downregulation appears to be an evolutionarily conserved (human and mouse) and oncogene-specific phenomenon in breast cancer"

    Article Title: Epigenetic CRBP downregulation appears to be an evolutionarily conserved (human and mouse) and oncogene-specific phenomenon in breast cancer

    Journal: Molecular Cancer

    doi: 10.1186/1476-4598-3-13

    In some human breast cancers, hCRBP hypermethylation is widespread. A. Sketch outlining the Southern blot strategy used to evaluate hCRBP methylation in human breast cancer specimens. The strategy was tested in MDA-MB-468 and ZR75-1 cells whose hCRBP methylation status was known beforehand. B, BH and BM stand for, respectively, Bsu36I digestion alone, Bsu36I digestion followed by Hpall digestion, and Bsu36I digestion followed by MspI digestion (MspI is the methylation-insensitive isoschizomer of Hpall). In agreement with the bisulfite sequencing data, Southern blotting revealed hCRBP undermethylation in MDA-MB-468 and hypermethylation in MCF-7 clone A cells (note loss of the large Bsu36I fragment after BH digestion of MDA-MB-468 genomic DNA and retention of this fragment in the case of MCF-7 clone A). A hCRBP restriction fragment length polymorphism (an extra Bsu36I site within the 205 bp fragment) accounts for the displaced band migration pattern in MDA-MB-468 cells. B. Southern, RT-PCR and Western blotting results for 2 human breast cancer specimens. The yield of the 205 bp product was decreased by > 50% after BH digestion relative to BM digestion. We infer from this that > 50% of the cells in the tumor displayed hCRBP hypermethylation. The protein blot was post-stained with Ponceau S and a major band used as loading control.
    Figure Legend Snippet: In some human breast cancers, hCRBP hypermethylation is widespread. A. Sketch outlining the Southern blot strategy used to evaluate hCRBP methylation in human breast cancer specimens. The strategy was tested in MDA-MB-468 and ZR75-1 cells whose hCRBP methylation status was known beforehand. B, BH and BM stand for, respectively, Bsu36I digestion alone, Bsu36I digestion followed by Hpall digestion, and Bsu36I digestion followed by MspI digestion (MspI is the methylation-insensitive isoschizomer of Hpall). In agreement with the bisulfite sequencing data, Southern blotting revealed hCRBP undermethylation in MDA-MB-468 and hypermethylation in MCF-7 clone A cells (note loss of the large Bsu36I fragment after BH digestion of MDA-MB-468 genomic DNA and retention of this fragment in the case of MCF-7 clone A). A hCRBP restriction fragment length polymorphism (an extra Bsu36I site within the 205 bp fragment) accounts for the displaced band migration pattern in MDA-MB-468 cells. B. Southern, RT-PCR and Western blotting results for 2 human breast cancer specimens. The yield of the 205 bp product was decreased by > 50% after BH digestion relative to BM digestion. We infer from this that > 50% of the cells in the tumor displayed hCRBP hypermethylation. The protein blot was post-stained with Ponceau S and a major band used as loading control.

    Techniques Used: Southern Blot, Methylation, Multiple Displacement Amplification, Methylation Sequencing, Migration, Reverse Transcription Polymerase Chain Reaction, Western Blot, Staining

    Related Articles

    Plasmid Preparation:

    Article Title: Cloning and characterization of tRNA (m1A58) methyltransferase (TrmI) from Thermus thermophilus HB27, a protein required for cell growth at extreme temperatures
    Article Snippet: In the resulting construct, pML11b, the knt (Kmr ) gene is transcribed from the 16S rRNA promoter. .. Plasmid pTT1 was digested with Bsu 36I (New England Biolabs, Beverly, MA). .. The 509 bp Bsu 36I fragment, in the coding region of trmI , was replaced by the 838 bp Bsu 36I fragment of pML11b to create the ligation product pML51 used for homologous recombination.

    Ethanol Precipitation:

    Article Title: The capacity to form H-DNA cannot substitute for GAGA factor binding to a (CT)n?(GA)n regulatory site
    Article Snippet: Five micrograms of supercoiled plasmid DNA were treated with 30 U of S1 nuclease (Promega) at room temperature for 15 min in S1 buffer (pH 5.0). .. After phenol extraction and ethanol precipitation, the samples were resuspended in 1× Bsu 36I buffer and were digested to completion with Bsu 36I (NEB). .. Linear controls were prepared by digestion with Bsu 36I and were subsequently treated with S1.

    other:

    Article Title: The capacity to form H-DNA cannot substitute for GAGA factor binding to a (CT)n?(GA)n regulatory site
    Article Snippet: Linear controls were prepared by digestion with Bsu 36I and were subsequently treated with S1.

    Sequencing:

    Article Title: Novel Isoforms of the Sodium Channels Nav1.8 and Nav1.5 Are Produced by a Conserved Mechanism in Mouse and Rat *
    Article Snippet: No product was detected in RT-minus controls, or by RT-PCR of neonatal heart RNA , as previously shown for rat and human heart ( , , ). .. Consistent with the novel cDNA sequence, there was no apparent digestion by Bsu36I either of the 435-bp seminested PCR products prior to cloning, or of 8 individual cDNA clones from each of the three DRG tissues (data not shown). ..

    Polymerase Chain Reaction:

    Article Title: Novel Isoforms of the Sodium Channels Nav1.8 and Nav1.5 Are Produced by a Conserved Mechanism in Mouse and Rat *
    Article Snippet: No product was detected in RT-minus controls, or by RT-PCR of neonatal heart RNA , as previously shown for rat and human heart ( , , ). .. Consistent with the novel cDNA sequence, there was no apparent digestion by Bsu36I either of the 435-bp seminested PCR products prior to cloning, or of 8 individual cDNA clones from each of the three DRG tissues (data not shown). ..

    Clone Assay:

    Article Title: Novel Isoforms of the Sodium Channels Nav1.8 and Nav1.5 Are Produced by a Conserved Mechanism in Mouse and Rat *
    Article Snippet: No product was detected in RT-minus controls, or by RT-PCR of neonatal heart RNA , as previously shown for rat and human heart ( , , ). .. Consistent with the novel cDNA sequence, there was no apparent digestion by Bsu36I either of the 435-bp seminested PCR products prior to cloning, or of 8 individual cDNA clones from each of the three DRG tissues (data not shown). ..

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    New England Biolabs bsu 36i
    Map of the hsp26-lacZ control transgene. The regulatory region of the hsp26 gene is enlarged to show the important regulatory sites. The (CT) n repeats (striped boxes), heat shock elements (filled boxes) and TATA box (open box) are indicated. The positions of the DNase I hypersensitive sites (DH sites) and the intervening nucleosome are shown below. The 1.1 kb proximal probe is used for quantifying accessibility at the proximal Xba I site and the 0.6 kb distal probe is used for quantifying accessibility at the distal Xba I site. The 0.5 kb Eco RI– <t>Bsu</t> <t>36I</t> fragment is used in indirect end-labeling experiments to determine S1 nuclease cleavage sites. The oligonucleotides shown below are those used in primer extension experiments to map the sites of chemical modification and UV-induced pyrimidine dimers. The direction of primer extension is indicated by the direction of each arrow. Oligo 5 hybridizes to vector sequences in plasmid pGhsp26.11 and the first extension products begin at the Alu I site at –143 (see Materials and Methods). Restriction sites: A, Alu I; B, Bsu 36I; F, Fsp I; H, Hin dIII; Hp, Hpa I; N, Nae I; RI, Eco RI; RV, Eco RV; S, Sma I; X, Xba I.
    Bsu 36i, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bsu 36i/product/New England Biolabs
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bsu 36i - by Bioz Stars, 2021-06
    96/100 stars
      Buy from Supplier

    Image Search Results


    Map of the hsp26-lacZ control transgene. The regulatory region of the hsp26 gene is enlarged to show the important regulatory sites. The (CT) n repeats (striped boxes), heat shock elements (filled boxes) and TATA box (open box) are indicated. The positions of the DNase I hypersensitive sites (DH sites) and the intervening nucleosome are shown below. The 1.1 kb proximal probe is used for quantifying accessibility at the proximal Xba I site and the 0.6 kb distal probe is used for quantifying accessibility at the distal Xba I site. The 0.5 kb Eco RI– Bsu 36I fragment is used in indirect end-labeling experiments to determine S1 nuclease cleavage sites. The oligonucleotides shown below are those used in primer extension experiments to map the sites of chemical modification and UV-induced pyrimidine dimers. The direction of primer extension is indicated by the direction of each arrow. Oligo 5 hybridizes to vector sequences in plasmid pGhsp26.11 and the first extension products begin at the Alu I site at –143 (see Materials and Methods). Restriction sites: A, Alu I; B, Bsu 36I; F, Fsp I; H, Hin dIII; Hp, Hpa I; N, Nae I; RI, Eco RI; RV, Eco RV; S, Sma I; X, Xba I.

    Journal: Nucleic Acids Research

    Article Title: The capacity to form H-DNA cannot substitute for GAGA factor binding to a (CT)n?(GA)n regulatory site

    doi:

    Figure Lengend Snippet: Map of the hsp26-lacZ control transgene. The regulatory region of the hsp26 gene is enlarged to show the important regulatory sites. The (CT) n repeats (striped boxes), heat shock elements (filled boxes) and TATA box (open box) are indicated. The positions of the DNase I hypersensitive sites (DH sites) and the intervening nucleosome are shown below. The 1.1 kb proximal probe is used for quantifying accessibility at the proximal Xba I site and the 0.6 kb distal probe is used for quantifying accessibility at the distal Xba I site. The 0.5 kb Eco RI– Bsu 36I fragment is used in indirect end-labeling experiments to determine S1 nuclease cleavage sites. The oligonucleotides shown below are those used in primer extension experiments to map the sites of chemical modification and UV-induced pyrimidine dimers. The direction of primer extension is indicated by the direction of each arrow. Oligo 5 hybridizes to vector sequences in plasmid pGhsp26.11 and the first extension products begin at the Alu I site at –143 (see Materials and Methods). Restriction sites: A, Alu I; B, Bsu 36I; F, Fsp I; H, Hin dIII; Hp, Hpa I; N, Nae I; RI, Eco RI; RV, Eco RV; S, Sma I; X, Xba I.

    Article Snippet: After phenol extraction and ethanol precipitation, the samples were resuspended in 1× Bsu 36I buffer and were digested to completion with Bsu 36I (NEB).

    Techniques: End Labeling, Modification, Plasmid Preparation

    In some human breast cancers, hCRBP hypermethylation is widespread. A. Sketch outlining the Southern blot strategy used to evaluate hCRBP methylation in human breast cancer specimens. The strategy was tested in MDA-MB-468 and ZR75-1 cells whose hCRBP methylation status was known beforehand. B, BH and BM stand for, respectively, Bsu36I digestion alone, Bsu36I digestion followed by Hpall digestion, and Bsu36I digestion followed by MspI digestion (MspI is the methylation-insensitive isoschizomer of Hpall). In agreement with the bisulfite sequencing data, Southern blotting revealed hCRBP undermethylation in MDA-MB-468 and hypermethylation in MCF-7 clone A cells (note loss of the large Bsu36I fragment after BH digestion of MDA-MB-468 genomic DNA and retention of this fragment in the case of MCF-7 clone A). A hCRBP restriction fragment length polymorphism (an extra Bsu36I site within the 205 bp fragment) accounts for the displaced band migration pattern in MDA-MB-468 cells. B. Southern, RT-PCR and Western blotting results for 2 human breast cancer specimens. The yield of the 205 bp product was decreased by > 50% after BH digestion relative to BM digestion. We infer from this that > 50% of the cells in the tumor displayed hCRBP hypermethylation. The protein blot was post-stained with Ponceau S and a major band used as loading control.

    Journal: Molecular Cancer

    Article Title: Epigenetic CRBP downregulation appears to be an evolutionarily conserved (human and mouse) and oncogene-specific phenomenon in breast cancer

    doi: 10.1186/1476-4598-3-13

    Figure Lengend Snippet: In some human breast cancers, hCRBP hypermethylation is widespread. A. Sketch outlining the Southern blot strategy used to evaluate hCRBP methylation in human breast cancer specimens. The strategy was tested in MDA-MB-468 and ZR75-1 cells whose hCRBP methylation status was known beforehand. B, BH and BM stand for, respectively, Bsu36I digestion alone, Bsu36I digestion followed by Hpall digestion, and Bsu36I digestion followed by MspI digestion (MspI is the methylation-insensitive isoschizomer of Hpall). In agreement with the bisulfite sequencing data, Southern blotting revealed hCRBP undermethylation in MDA-MB-468 and hypermethylation in MCF-7 clone A cells (note loss of the large Bsu36I fragment after BH digestion of MDA-MB-468 genomic DNA and retention of this fragment in the case of MCF-7 clone A). A hCRBP restriction fragment length polymorphism (an extra Bsu36I site within the 205 bp fragment) accounts for the displaced band migration pattern in MDA-MB-468 cells. B. Southern, RT-PCR and Western blotting results for 2 human breast cancer specimens. The yield of the 205 bp product was decreased by > 50% after BH digestion relative to BM digestion. We infer from this that > 50% of the cells in the tumor displayed hCRBP hypermethylation. The protein blot was post-stained with Ponceau S and a major band used as loading control.

    Article Snippet: Tumor DNA (45 μg) was digested with Bsu36I, ethanol precipitated, and divided into 3 parts that were either not digested further, digested with HpaII, or digested with MsPI (restriction enzymes were from New England Biolabs, Beverly, MA).

    Techniques: Southern Blot, Methylation, Multiple Displacement Amplification, Methylation Sequencing, Migration, Reverse Transcription Polymerase Chain Reaction, Western Blot, Staining