bstyi  (New England Biolabs)


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    Structured Review

    New England Biolabs bstyi
    Characterization of PRKCA D463H mutation in chordoid glioma. a Left panel: gadolinium T1 weighted MRI showing a well-limited, strongly enhancing mass developed at the anterior part of the third ventricule (patient 2123). Right panel: Pan-cancer PRKCA somatic mutations in TCGA cohort. D463H was not identified in this cohort and its position is marked by the straight line. b Somatic mutations in PRKCA in Cosmic census genes for the ChG samples with matched blood (G1 group). The predicted impact of the mutations according to Sift 7 and Polyphen 8 scores are given. c Three-dimensional structure of the catalytic site of PKCα showing the positions of D463 in the wild-type, H463 in the mutant protein, and the amino acids predicted to interact via hydrogen bonds with wild-type and mutant 463 residue. Hydrogen bonds identified using CHARMM software and Baker and Hubbard algorithm in MDTraj 70 python package (see Methods) are indicated by arrows: orange (common to both WT and mutant), blue (present only in WT), red (present only in the mutant). d PRKCA D463H transcript is overexpressed in ChG compared to PRKCA WT . Left: the G to C substitution leads to the loss of <t>BstYI</t> restriction site in the mutant allele. <t>PCR</t> products from ChG cDNA were digested by BstYI then analyzed by LabChIP GX. Lanes T7033 and T7095 correspond to cDNA from low-grade glioma wild type for PRKCA , which shows that digestion efficiency is 100%. Mutant product size appears arround 418 bp; wild type digested product sizes appears around 227 and 185 bp. The last two lanes correspond to pcDNA plasmid controls, either WT or D463H mutant (MUT). Right: concentration ratio of mutant to wild-type bands is indicated (mean = 1.8)
    Bstyi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bstyi/product/New England Biolabs
    Average 93 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    bstyi - by Bioz Stars, 2022-11
    93/100 stars

    Images

    1) Product Images from "A recurrent point mutation in PRKCA is a hallmark of chordoid gliomas"

    Article Title: A recurrent point mutation in PRKCA is a hallmark of chordoid gliomas

    Journal: Nature Communications

    doi: 10.1038/s41467-018-04622-w

    Characterization of PRKCA D463H mutation in chordoid glioma. a Left panel: gadolinium T1 weighted MRI showing a well-limited, strongly enhancing mass developed at the anterior part of the third ventricule (patient 2123). Right panel: Pan-cancer PRKCA somatic mutations in TCGA cohort. D463H was not identified in this cohort and its position is marked by the straight line. b Somatic mutations in PRKCA in Cosmic census genes for the ChG samples with matched blood (G1 group). The predicted impact of the mutations according to Sift 7 and Polyphen 8 scores are given. c Three-dimensional structure of the catalytic site of PKCα showing the positions of D463 in the wild-type, H463 in the mutant protein, and the amino acids predicted to interact via hydrogen bonds with wild-type and mutant 463 residue. Hydrogen bonds identified using CHARMM software and Baker and Hubbard algorithm in MDTraj 70 python package (see Methods) are indicated by arrows: orange (common to both WT and mutant), blue (present only in WT), red (present only in the mutant). d PRKCA D463H transcript is overexpressed in ChG compared to PRKCA WT . Left: the G to C substitution leads to the loss of BstYI restriction site in the mutant allele. PCR products from ChG cDNA were digested by BstYI then analyzed by LabChIP GX. Lanes T7033 and T7095 correspond to cDNA from low-grade glioma wild type for PRKCA , which shows that digestion efficiency is 100%. Mutant product size appears arround 418 bp; wild type digested product sizes appears around 227 and 185 bp. The last two lanes correspond to pcDNA plasmid controls, either WT or D463H mutant (MUT). Right: concentration ratio of mutant to wild-type bands is indicated (mean = 1.8)
    Figure Legend Snippet: Characterization of PRKCA D463H mutation in chordoid glioma. a Left panel: gadolinium T1 weighted MRI showing a well-limited, strongly enhancing mass developed at the anterior part of the third ventricule (patient 2123). Right panel: Pan-cancer PRKCA somatic mutations in TCGA cohort. D463H was not identified in this cohort and its position is marked by the straight line. b Somatic mutations in PRKCA in Cosmic census genes for the ChG samples with matched blood (G1 group). The predicted impact of the mutations according to Sift 7 and Polyphen 8 scores are given. c Three-dimensional structure of the catalytic site of PKCα showing the positions of D463 in the wild-type, H463 in the mutant protein, and the amino acids predicted to interact via hydrogen bonds with wild-type and mutant 463 residue. Hydrogen bonds identified using CHARMM software and Baker and Hubbard algorithm in MDTraj 70 python package (see Methods) are indicated by arrows: orange (common to both WT and mutant), blue (present only in WT), red (present only in the mutant). d PRKCA D463H transcript is overexpressed in ChG compared to PRKCA WT . Left: the G to C substitution leads to the loss of BstYI restriction site in the mutant allele. PCR products from ChG cDNA were digested by BstYI then analyzed by LabChIP GX. Lanes T7033 and T7095 correspond to cDNA from low-grade glioma wild type for PRKCA , which shows that digestion efficiency is 100%. Mutant product size appears arround 418 bp; wild type digested product sizes appears around 227 and 185 bp. The last two lanes correspond to pcDNA plasmid controls, either WT or D463H mutant (MUT). Right: concentration ratio of mutant to wild-type bands is indicated (mean = 1.8)

    Techniques Used: Mutagenesis, Magnetic Resonance Imaging, Software, Polymerase Chain Reaction, Plasmid Preparation, Concentration Assay

    2) Product Images from "Distribution of BoLA-DRB3 Allelic Frequencies and Identification of Two New Alleles in Iranian Buffalo Breed"

    Article Title: Distribution of BoLA-DRB3 Allelic Frequencies and Identification of Two New Alleles in Iranian Buffalo Breed

    Journal: The Scientific World Journal

    doi: 10.1100/2012/863024

    Electrophoresis in 8% polyacrylamide gel of exon 2 amplification products of gene BoLA-DRB3 digested by endonucleases Rsa I (b), Hae III (a), and BstYI (c). Msp I fragments of plasmid pUC19 are used as a molecular marker. The length of fragments composing Rsa I, Hae III, or Bst YI DNA patterns is shown on pictures.
    Figure Legend Snippet: Electrophoresis in 8% polyacrylamide gel of exon 2 amplification products of gene BoLA-DRB3 digested by endonucleases Rsa I (b), Hae III (a), and BstYI (c). Msp I fragments of plasmid pUC19 are used as a molecular marker. The length of fragments composing Rsa I, Hae III, or Bst YI DNA patterns is shown on pictures.

    Techniques Used: Electrophoresis, Amplification, Plasmid Preparation, Marker

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    New England Biolabs bstyi restriction enzyme
    Bstyi Restriction Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bstyi restriction enzyme/product/New England Biolabs
    Average 94 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    bstyi restriction enzyme - by Bioz Stars, 2022-11
    94/100 stars
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