bstyi  (New England Biolabs)


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    Name:
    BstYI
    Description:
    BstYI 10 000 units
    Catalog Number:
    r0523l
    Price:
    269
    Category:
    Restriction Enzymes
    Size:
    10 000 units
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    Structured Review

    New England Biolabs bstyi
    BstYI
    BstYI 10 000 units
    https://www.bioz.com/result/bstyi/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bstyi - by Bioz Stars, 2021-03
    93/100 stars

    Images

    1) Product Images from "Distribution of BoLA-DRB3 Allelic Frequencies and Identification of Two New Alleles in Iranian Buffalo Breed"

    Article Title: Distribution of BoLA-DRB3 Allelic Frequencies and Identification of Two New Alleles in Iranian Buffalo Breed

    Journal: The Scientific World Journal

    doi: 10.1100/2012/863024

    Electrophoresis in 8% polyacrylamide gel of exon 2 amplification products of gene BoLA-DRB3 digested by endonucleases Rsa I (b), Hae III (a), and BstYI (c). Msp I fragments of plasmid pUC19 are used as a molecular marker. The length of fragments composing Rsa I, Hae III, or Bst YI DNA patterns is shown on pictures.
    Figure Legend Snippet: Electrophoresis in 8% polyacrylamide gel of exon 2 amplification products of gene BoLA-DRB3 digested by endonucleases Rsa I (b), Hae III (a), and BstYI (c). Msp I fragments of plasmid pUC19 are used as a molecular marker. The length of fragments composing Rsa I, Hae III, or Bst YI DNA patterns is shown on pictures.

    Techniques Used: Electrophoresis, Amplification, Plasmid Preparation, Marker

    2) Product Images from "A recurrent point mutation in PRKCA is a hallmark of chordoid gliomas"

    Article Title: A recurrent point mutation in PRKCA is a hallmark of chordoid gliomas

    Journal: Nature Communications

    doi: 10.1038/s41467-018-04622-w

    Characterization of PRKCA D463H mutation in chordoid glioma. a Left panel: gadolinium T1 weighted MRI showing a well-limited, strongly enhancing mass developed at the anterior part of the third ventricule (patient 2123). Right panel: Pan-cancer PRKCA somatic mutations in TCGA cohort. D463H was not identified in this cohort and its position is marked by the straight line. b Somatic mutations in PRKCA in Cosmic census genes for the ChG samples with matched blood (G1 group). The predicted impact of the mutations according to Sift 7 and Polyphen 8 scores are given. c Three-dimensional structure of the catalytic site of PKCα showing the positions of D463 in the wild-type, H463 in the mutant protein, and the amino acids predicted to interact via hydrogen bonds with wild-type and mutant 463 residue. Hydrogen bonds identified using CHARMM software and Baker and Hubbard algorithm in MDTraj 70 python package (see Methods) are indicated by arrows: orange (common to both WT and mutant), blue (present only in WT), red (present only in the mutant). d PRKCA D463H transcript is overexpressed in ChG compared to PRKCA WT . Left: the G to C substitution leads to the loss of BstYI restriction site in the mutant allele. PCR products from ChG cDNA were digested by BstYI then analyzed by LabChIP GX. Lanes T7033 and T7095 correspond to cDNA from low-grade glioma wild type for PRKCA , which shows that digestion efficiency is 100%. Mutant product size appears arround 418 bp; wild type digested product sizes appears around 227 and 185 bp. The last two lanes correspond to pcDNA plasmid controls, either WT or D463H mutant (MUT). Right: concentration ratio of mutant to wild-type bands is indicated (mean = 1.8)
    Figure Legend Snippet: Characterization of PRKCA D463H mutation in chordoid glioma. a Left panel: gadolinium T1 weighted MRI showing a well-limited, strongly enhancing mass developed at the anterior part of the third ventricule (patient 2123). Right panel: Pan-cancer PRKCA somatic mutations in TCGA cohort. D463H was not identified in this cohort and its position is marked by the straight line. b Somatic mutations in PRKCA in Cosmic census genes for the ChG samples with matched blood (G1 group). The predicted impact of the mutations according to Sift 7 and Polyphen 8 scores are given. c Three-dimensional structure of the catalytic site of PKCα showing the positions of D463 in the wild-type, H463 in the mutant protein, and the amino acids predicted to interact via hydrogen bonds with wild-type and mutant 463 residue. Hydrogen bonds identified using CHARMM software and Baker and Hubbard algorithm in MDTraj 70 python package (see Methods) are indicated by arrows: orange (common to both WT and mutant), blue (present only in WT), red (present only in the mutant). d PRKCA D463H transcript is overexpressed in ChG compared to PRKCA WT . Left: the G to C substitution leads to the loss of BstYI restriction site in the mutant allele. PCR products from ChG cDNA were digested by BstYI then analyzed by LabChIP GX. Lanes T7033 and T7095 correspond to cDNA from low-grade glioma wild type for PRKCA , which shows that digestion efficiency is 100%. Mutant product size appears arround 418 bp; wild type digested product sizes appears around 227 and 185 bp. The last two lanes correspond to pcDNA plasmid controls, either WT or D463H mutant (MUT). Right: concentration ratio of mutant to wild-type bands is indicated (mean = 1.8)

    Techniques Used: Mutagenesis, Magnetic Resonance Imaging, Software, Polymerase Chain Reaction, Plasmid Preparation, Concentration Assay

    Related Articles

    Clone Assay:

    Article Title: Tropomyosin isoforms differentially affect muscle contractility in the head and body regions of the nematode Caenorhabditis elegans
    Article Snippet: .. Cloning of the lev-11o cDNA First-strand cDNAs were reverse-transcribed from total RNAs from the N2 strain using oligo-dT by a Maxima H- First Strand cDNA Synthesis Kit (Thermo Fisher Scientific). lev-11 cDNAs were amplified by PCR using a primer pair for E1 and E9c with Platinum Taq DNA polymerase (Thermo Fisher Scientific), purified by a PCR Cleanup kit (Qiagen), and digested by Bst YI (New England BioLabs) that specifically recognized E7b. .. Bst YI-resistant cDNAs were amplified by PCR using the same primer set and cloned in a pET-3d plasmid vector (EMD MilliporeA) by an In-Fusion HD Cloning kit (Takara Bio USA).

    Amplification:

    Article Title: Tropomyosin isoforms differentially affect muscle contractility in the head and body regions of the nematode Caenorhabditis elegans
    Article Snippet: .. Cloning of the lev-11o cDNA First-strand cDNAs were reverse-transcribed from total RNAs from the N2 strain using oligo-dT by a Maxima H- First Strand cDNA Synthesis Kit (Thermo Fisher Scientific). lev-11 cDNAs were amplified by PCR using a primer pair for E1 and E9c with Platinum Taq DNA polymerase (Thermo Fisher Scientific), purified by a PCR Cleanup kit (Qiagen), and digested by Bst YI (New England BioLabs) that specifically recognized E7b. .. Bst YI-resistant cDNAs were amplified by PCR using the same primer set and cloned in a pET-3d plasmid vector (EMD MilliporeA) by an In-Fusion HD Cloning kit (Takara Bio USA).

    Polymerase Chain Reaction:

    Article Title: Tropomyosin isoforms differentially affect muscle contractility in the head and body regions of the nematode Caenorhabditis elegans
    Article Snippet: .. Cloning of the lev-11o cDNA First-strand cDNAs were reverse-transcribed from total RNAs from the N2 strain using oligo-dT by a Maxima H- First Strand cDNA Synthesis Kit (Thermo Fisher Scientific). lev-11 cDNAs were amplified by PCR using a primer pair for E1 and E9c with Platinum Taq DNA polymerase (Thermo Fisher Scientific), purified by a PCR Cleanup kit (Qiagen), and digested by Bst YI (New England BioLabs) that specifically recognized E7b. .. Bst YI-resistant cDNAs were amplified by PCR using the same primer set and cloned in a pET-3d plasmid vector (EMD MilliporeA) by an In-Fusion HD Cloning kit (Takara Bio USA).

    Article Title: A recurrent point mutation in PRKCA is a hallmark of chordoid gliomas
    Article Snippet: RT-PCR RT-PCR detection of PRKCA D463H mutant cDNA: cDNA were retrotranscribed from tumor RNA samples (1 µg) with Maxima First Strand cDNA Synthesis Kit for RT-qPCR (Thermo Fisher Scientific K1641) according to manufacturer instructions, then amplified with PCR primers VG-229 and VG-623 by using Fast Start PCR Master (Roche 04710452001). .. PCR reactions were split and half was digested with BstYI (New England Biolabs), when the other half was incubated in the digestion mix without BstYI, and left over night at 60 °C. .. Fragments were analyzed on 2% agarose gel or by using LabChip GX bioanalyzer (Caliper LifeSciences, Villepinte, France).

    Article Title: Distribution of BoLA-DRB3 Allelic Frequencies and Identification of Two New Alleles in Iranian Buffalo Breed
    Article Snippet: .. Restriction Endonuclease Digestion The PCR-amplified DNA fragments from the second PCR restriction were digested with restriction endonuclease Rsa I, BstYI , and Hae III (New England Biolabs, Beverly, Mass). .. For the restriction endonuclease digestion restriction, 10 μ L of the second PCR restriction product was used for each digestion.

    Purification:

    Article Title: Tropomyosin isoforms differentially affect muscle contractility in the head and body regions of the nematode Caenorhabditis elegans
    Article Snippet: .. Cloning of the lev-11o cDNA First-strand cDNAs were reverse-transcribed from total RNAs from the N2 strain using oligo-dT by a Maxima H- First Strand cDNA Synthesis Kit (Thermo Fisher Scientific). lev-11 cDNAs were amplified by PCR using a primer pair for E1 and E9c with Platinum Taq DNA polymerase (Thermo Fisher Scientific), purified by a PCR Cleanup kit (Qiagen), and digested by Bst YI (New England BioLabs) that specifically recognized E7b. .. Bst YI-resistant cDNAs were amplified by PCR using the same primer set and cloned in a pET-3d plasmid vector (EMD MilliporeA) by an In-Fusion HD Cloning kit (Takara Bio USA).

    Isolation:

    Article Title: Hematopoietic Immortalizing Function of the NKL-Subclass Homeobox Gene TLX1
    Article Snippet: Linker-mediated nested polymerase chain reaction (LM-PCR) was performed as previously described ( ; ) with modifications that allowed the identification of genomic regions adjacent to MSCVneo-TLX1 vector sequences in a murine background. .. Briefly, genomic DNA was isolated from cell lines using the GenElute Mammalian Genomic DNA Kit and digested with Tsp 509I or Bst YI (New England Biolabs). ..

    Incubation:

    Article Title: A recurrent point mutation in PRKCA is a hallmark of chordoid gliomas
    Article Snippet: RT-PCR RT-PCR detection of PRKCA D463H mutant cDNA: cDNA were retrotranscribed from tumor RNA samples (1 µg) with Maxima First Strand cDNA Synthesis Kit for RT-qPCR (Thermo Fisher Scientific K1641) according to manufacturer instructions, then amplified with PCR primers VG-229 and VG-623 by using Fast Start PCR Master (Roche 04710452001). .. PCR reactions were split and half was digested with BstYI (New England Biolabs), when the other half was incubated in the digestion mix without BstYI, and left over night at 60 °C. .. Fragments were analyzed on 2% agarose gel or by using LabChip GX bioanalyzer (Caliper LifeSciences, Villepinte, France).

    Agarose Gel Electrophoresis:

    Article Title: Data from 11 years of molecular typing infectious bronchitis virus field isolates.
    Article Snippet: The S1 amplicons were gel purified on a 1% agarose gel using GenElute spin columns (Sigma-Aldrich Co., St. Louis, MO) and Microcon 30 columns (Millipore, Bedford, MA) per the manufacturer’s instructions and then used for the RFLP analysis. .. Restriction enzymes BstYI, HaeIII, and XcmI (New England Biolabs, Inc., Beverly, MA) were used in separate reactions according to the manufacturer’s instructions to digest the amplicons, and the resulting DNA fragments were separated on a 1% agarose gel and visualized with EtBr staining and an ultraviolet light transilluminator. ..

    Staining:

    Article Title: Data from 11 years of molecular typing infectious bronchitis virus field isolates.
    Article Snippet: The S1 amplicons were gel purified on a 1% agarose gel using GenElute spin columns (Sigma-Aldrich Co., St. Louis, MO) and Microcon 30 columns (Millipore, Bedford, MA) per the manufacturer’s instructions and then used for the RFLP analysis. .. Restriction enzymes BstYI, HaeIII, and XcmI (New England Biolabs, Inc., Beverly, MA) were used in separate reactions according to the manufacturer’s instructions to digest the amplicons, and the resulting DNA fragments were separated on a 1% agarose gel and visualized with EtBr staining and an ultraviolet light transilluminator. ..

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    New England Biolabs bstyi
    Electrophoresis in 8% polyacrylamide gel of exon 2 amplification products of gene BoLA-DRB3 digested by endonucleases <t>Rsa</t> I (b), Hae III (a), and <t>BstYI</t> (c). Msp I fragments of plasmid pUC19 are used as a molecular marker. The length of fragments composing Rsa I, Hae III, or Bst YI DNA patterns is shown on pictures.
    Bstyi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bstyi/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bstyi - by Bioz Stars, 2021-03
    93/100 stars
      Buy from Supplier

    Image Search Results


    Electrophoresis in 8% polyacrylamide gel of exon 2 amplification products of gene BoLA-DRB3 digested by endonucleases Rsa I (b), Hae III (a), and BstYI (c). Msp I fragments of plasmid pUC19 are used as a molecular marker. The length of fragments composing Rsa I, Hae III, or Bst YI DNA patterns is shown on pictures.

    Journal: The Scientific World Journal

    Article Title: Distribution of BoLA-DRB3 Allelic Frequencies and Identification of Two New Alleles in Iranian Buffalo Breed

    doi: 10.1100/2012/863024

    Figure Lengend Snippet: Electrophoresis in 8% polyacrylamide gel of exon 2 amplification products of gene BoLA-DRB3 digested by endonucleases Rsa I (b), Hae III (a), and BstYI (c). Msp I fragments of plasmid pUC19 are used as a molecular marker. The length of fragments composing Rsa I, Hae III, or Bst YI DNA patterns is shown on pictures.

    Article Snippet: Restriction Endonuclease Digestion The PCR-amplified DNA fragments from the second PCR restriction were digested with restriction endonuclease Rsa I, BstYI , and Hae III (New England Biolabs, Beverly, Mass).

    Techniques: Electrophoresis, Amplification, Plasmid Preparation, Marker

    Characterization of PRKCA D463H mutation in chordoid glioma. a Left panel: gadolinium T1 weighted MRI showing a well-limited, strongly enhancing mass developed at the anterior part of the third ventricule (patient 2123). Right panel: Pan-cancer PRKCA somatic mutations in TCGA cohort. D463H was not identified in this cohort and its position is marked by the straight line. b Somatic mutations in PRKCA in Cosmic census genes for the ChG samples with matched blood (G1 group). The predicted impact of the mutations according to Sift 7 and Polyphen 8 scores are given. c Three-dimensional structure of the catalytic site of PKCα showing the positions of D463 in the wild-type, H463 in the mutant protein, and the amino acids predicted to interact via hydrogen bonds with wild-type and mutant 463 residue. Hydrogen bonds identified using CHARMM software and Baker and Hubbard algorithm in MDTraj 70 python package (see Methods) are indicated by arrows: orange (common to both WT and mutant), blue (present only in WT), red (present only in the mutant). d PRKCA D463H transcript is overexpressed in ChG compared to PRKCA WT . Left: the G to C substitution leads to the loss of BstYI restriction site in the mutant allele. PCR products from ChG cDNA were digested by BstYI then analyzed by LabChIP GX. Lanes T7033 and T7095 correspond to cDNA from low-grade glioma wild type for PRKCA , which shows that digestion efficiency is 100%. Mutant product size appears arround 418 bp; wild type digested product sizes appears around 227 and 185 bp. The last two lanes correspond to pcDNA plasmid controls, either WT or D463H mutant (MUT). Right: concentration ratio of mutant to wild-type bands is indicated (mean = 1.8)

    Journal: Nature Communications

    Article Title: A recurrent point mutation in PRKCA is a hallmark of chordoid gliomas

    doi: 10.1038/s41467-018-04622-w

    Figure Lengend Snippet: Characterization of PRKCA D463H mutation in chordoid glioma. a Left panel: gadolinium T1 weighted MRI showing a well-limited, strongly enhancing mass developed at the anterior part of the third ventricule (patient 2123). Right panel: Pan-cancer PRKCA somatic mutations in TCGA cohort. D463H was not identified in this cohort and its position is marked by the straight line. b Somatic mutations in PRKCA in Cosmic census genes for the ChG samples with matched blood (G1 group). The predicted impact of the mutations according to Sift 7 and Polyphen 8 scores are given. c Three-dimensional structure of the catalytic site of PKCα showing the positions of D463 in the wild-type, H463 in the mutant protein, and the amino acids predicted to interact via hydrogen bonds with wild-type and mutant 463 residue. Hydrogen bonds identified using CHARMM software and Baker and Hubbard algorithm in MDTraj 70 python package (see Methods) are indicated by arrows: orange (common to both WT and mutant), blue (present only in WT), red (present only in the mutant). d PRKCA D463H transcript is overexpressed in ChG compared to PRKCA WT . Left: the G to C substitution leads to the loss of BstYI restriction site in the mutant allele. PCR products from ChG cDNA were digested by BstYI then analyzed by LabChIP GX. Lanes T7033 and T7095 correspond to cDNA from low-grade glioma wild type for PRKCA , which shows that digestion efficiency is 100%. Mutant product size appears arround 418 bp; wild type digested product sizes appears around 227 and 185 bp. The last two lanes correspond to pcDNA plasmid controls, either WT or D463H mutant (MUT). Right: concentration ratio of mutant to wild-type bands is indicated (mean = 1.8)

    Article Snippet: PCR reactions were split and half was digested with BstYI (New England Biolabs), when the other half was incubated in the digestion mix without BstYI, and left over night at 60 °C.

    Techniques: Mutagenesis, Magnetic Resonance Imaging, Software, Polymerase Chain Reaction, Plasmid Preparation, Concentration Assay

    3C assay. ( A ) Genomic DNA products of BstY I digestion (arrows), with areas of interest (HSS and gene promoters) labeled A–F and PCR primers numbered 1 and 2. ( B and C ) Relative cross-linking frequencies (±SEM) between the indicated fragments

    Journal:

    Article Title: Activation-dependent intrachromosomal interactions formed by the TNF gene promoter and two distal enhancers

    doi: 10.1073/pnas.0708210104

    Figure Lengend Snippet: 3C assay. ( A ) Genomic DNA products of BstY I digestion (arrows), with areas of interest (HSS and gene promoters) labeled A–F and PCR primers numbered 1 and 2. ( B and C ) Relative cross-linking frequencies (±SEM) between the indicated fragments

    Article Snippet: Nuclei were pelleted and digested with BstY I (New England BioLabs) for 3 h at 60°C (inactivated at 80°C for 20 min).

    Techniques: Labeling, Polymerase Chain Reaction