bstyi (New England Biolabs)


Name:
BstYI
Description:
BstYI 10 000 units
Catalog Number:
r0523l
Price:
269
Category:
Restriction Enzymes
Size:
10 000 units
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Structured Review

BstYI 10 000 units
https://www.bioz.com/result/bstyi/product/New England Biolabs
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Distribution of BoLA-DRB3 Allelic Frequencies and Identification of Two New Alleles in Iranian Buffalo Breed"
Article Title: Distribution of BoLA-DRB3 Allelic Frequencies and Identification of Two New Alleles in Iranian Buffalo Breed
Journal: The Scientific World Journal
doi: 10.1100/2012/863024

Figure Legend Snippet: Electrophoresis in 8% polyacrylamide gel of exon 2 amplification products of gene BoLA-DRB3 digested by endonucleases Rsa I (b), Hae III (a), and BstYI (c). Msp I fragments of plasmid pUC19 are used as a molecular marker. The length of fragments composing Rsa I, Hae III, or Bst YI DNA patterns is shown on pictures.
Techniques Used: Electrophoresis, Amplification, Plasmid Preparation, Marker
2) Product Images from "A recurrent point mutation in PRKCA is a hallmark of chordoid gliomas"
Article Title: A recurrent point mutation in PRKCA is a hallmark of chordoid gliomas
Journal: Nature Communications
doi: 10.1038/s41467-018-04622-w

Figure Legend Snippet: Characterization of PRKCA D463H mutation in chordoid glioma. a Left panel: gadolinium T1 weighted MRI showing a well-limited, strongly enhancing mass developed at the anterior part of the third ventricule (patient 2123). Right panel: Pan-cancer PRKCA somatic mutations in TCGA cohort. D463H was not identified in this cohort and its position is marked by the straight line. b Somatic mutations in PRKCA in Cosmic census genes for the ChG samples with matched blood (G1 group). The predicted impact of the mutations according to Sift 7 and Polyphen 8 scores are given. c Three-dimensional structure of the catalytic site of PKCα showing the positions of D463 in the wild-type, H463 in the mutant protein, and the amino acids predicted to interact via hydrogen bonds with wild-type and mutant 463 residue. Hydrogen bonds identified using CHARMM software and Baker and Hubbard algorithm in MDTraj 70 python package (see Methods) are indicated by arrows: orange (common to both WT and mutant), blue (present only in WT), red (present only in the mutant). d PRKCA D463H transcript is overexpressed in ChG compared to PRKCA WT . Left: the G to C substitution leads to the loss of BstYI restriction site in the mutant allele. PCR products from ChG cDNA were digested by BstYI then analyzed by LabChIP GX. Lanes T7033 and T7095 correspond to cDNA from low-grade glioma wild type for PRKCA , which shows that digestion efficiency is 100%. Mutant product size appears arround 418 bp; wild type digested product sizes appears around 227 and 185 bp. The last two lanes correspond to pcDNA plasmid controls, either WT or D463H mutant (MUT). Right: concentration ratio of mutant to wild-type bands is indicated (mean = 1.8)
Techniques Used: Mutagenesis, Magnetic Resonance Imaging, Software, Polymerase Chain Reaction, Plasmid Preparation, Concentration Assay
Related Articles
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