ple i  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96
    Name:
    PleI
    Description:
    PleI 1 000 units
    Catalog Number:
    r0515s
    Price:
    66
    Size:
    1 000 units
    Category:
    Restriction Enzymes
    Buy from Supplier


    Structured Review

    New England Biolabs ple i
    PleI
    PleI 1 000 units
    https://www.bioz.com/result/ple i/product/New England Biolabs
    Average 96 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    ple i - by Bioz Stars, 2020-07
    96/100 stars

    Images

    1) Product Images from "Sequence Conservation of Glycerophosphodiester Phosphodiesterase among Treponema pallidum Strains"

    Article Title: Sequence Conservation of Glycerophosphodiester Phosphodiesterase among Treponema pallidum Strains

    Journal: Infection and Immunity

    doi:

    RFLP analysis of the gpd amplicons from various pathogenic treponeme strains. The gpd open reading frame was amplified from each of the specified strains, digested with Ple I, and subjected to agarose gel electrophoresis and ethidium bromide staining. The left lane shows the 100-bp DNA ladder (New England Biolabs). Shown also is the undigested gpd amplicon from the Nichols strain. The sizes, in base pairs, of the DNA fragments generated by Ple I digestion of the gpd amplicons from the various strains are shown on the right.
    Figure Legend Snippet: RFLP analysis of the gpd amplicons from various pathogenic treponeme strains. The gpd open reading frame was amplified from each of the specified strains, digested with Ple I, and subjected to agarose gel electrophoresis and ethidium bromide staining. The left lane shows the 100-bp DNA ladder (New England Biolabs). Shown also is the undigested gpd amplicon from the Nichols strain. The sizes, in base pairs, of the DNA fragments generated by Ple I digestion of the gpd amplicons from the various strains are shown on the right.

    Techniques Used: Amplification, Agarose Gel Electrophoresis, Staining, Generated

    Related Articles

    Amplification:

    Article Title: Sequence Conservation of Glycerophosphodiester Phosphodiesterase among Treponema pallidum Strains
    Article Snippet: .. One microgram of each of the amplified templates was digested with Ple I (New England Biolabs, Beverly, Mass.) for 4 h at 37°C prior to electrophoresis on a 1.5% NuSieve (FMC BioProducts, Rockland, Maine) agarose gel. ..

    Agarose Gel Electrophoresis:

    Article Title: Sequence Conservation of Glycerophosphodiester Phosphodiesterase among Treponema pallidum Strains
    Article Snippet: .. One microgram of each of the amplified templates was digested with Ple I (New England Biolabs, Beverly, Mass.) for 4 h at 37°C prior to electrophoresis on a 1.5% NuSieve (FMC BioProducts, Rockland, Maine) agarose gel. ..

    Labeling:

    Article Title: Methylation Warfare: Interaction of Pneumococcal Bacteriophages with Their Host
    Article Snippet: .. A double digestion of the PCR products, using restriction enzymes DraI and PleI (New England Biolabs), allowed for the generation of labeled DNA fragments of different lengths, one specifically for each of the hsdS variant forms. ..

    Electrophoresis:

    Article Title: Sequence Conservation of Glycerophosphodiester Phosphodiesterase among Treponema pallidum Strains
    Article Snippet: .. One microgram of each of the amplified templates was digested with Ple I (New England Biolabs, Beverly, Mass.) for 4 h at 37°C prior to electrophoresis on a 1.5% NuSieve (FMC BioProducts, Rockland, Maine) agarose gel. ..

    Concentration Assay:

    Article Title: Phase variation in pneumococcal populations during carriage in the human nasopharynx
    Article Snippet: .. 10–15 µl (depending on DNA concentration) of PCR product was digested following the manufacturer’s instructions using 1U DraI (New England Biolabs, UK), 2U PleI (New England Biolabs, UK), 1X CutSmart Buffer (New England Biolabs, UK) in a total volume of 20 µl. .. Following digestion each FAM labelled SpnIII fragment present in the amplification mix has a unique size that can be distinguished through capillary electrophoresis on an ABI prism Gene Analyser (Applied Biosystems, USA).

    Incubation:

    Article Title: Capturing the primordial Kras mutation initiating urethane carcinogenesis
    Article Snippet: .. Twenty to fifty micrograms of genomic DNA was incubated with StuI (NEB) for analysis of the transcribed strand of Kras exon 1, EcoRV (NEB) and EcoRI (NEB) for analysis of the non-transcribed strand of Kras exon 1, XmnI (NEB) for analysis of the non-transcribed strand of Kras exon 2, and PleI (NEB) for analysis of the transcribed strand of Kras exon 2, or HphI (NEB) for the analysis of the non-transcribed strand of Hras exon 2. ..

    Polymerase Chain Reaction:

    Article Title: Deletion of the Zinc Transporter Lipoprotein AdcAII Causes Hyperencapsulation of Streptococcus pneumoniae Associated with Distinct Alleles of the Type I Restriction-Modification System
    Article Snippet: .. PCR products were digested using 1 U DraI, 2 U PleI, and 1× CutSmart Buffer (all from New England Biolabs, United Kingdom) in a 20-μl volume. .. Each FAM-labeled SpnIII variant has a unique size that can be distinguished through capillary electrophoresis on an ABI Prism gene analyzer (Applied Biosystems, USA) and analyzed using Peak Scanner v1.0 software.

    Article Title: Recombination of the Phase-Variable spnIII Locus Is Independent of All Known Pneumococcal Site-Specific Recombinases
    Article Snippet: .. A total of 10 to 15 μl of the PCR product was digested according to the manufacturer’s instructions, using 1 U DraI (New England Biolabs, UK), 2 U PleI (New England Biolabs, UK), and 1× CutSmart buffer (New England Biolabs, UK) in a total volume of 20 μl. .. Following digestion, each 6-carboxyfluorescein (FAM)-labeled SpnIII variant has a unique size (Table S3) that can be distinguished by capillary electrophoresis on an ABI prism gene analyzer (Applied Biosystems, USA).

    Article Title: Phase variation in pneumococcal populations during carriage in the human nasopharynx
    Article Snippet: .. 10–15 µl (depending on DNA concentration) of PCR product was digested following the manufacturer’s instructions using 1U DraI (New England Biolabs, UK), 2U PleI (New England Biolabs, UK), 1X CutSmart Buffer (New England Biolabs, UK) in a total volume of 20 µl. .. Following digestion each FAM labelled SpnIII fragment present in the amplification mix has a unique size that can be distinguished through capillary electrophoresis on an ABI prism Gene Analyser (Applied Biosystems, USA).

    Article Title: A random six-phase switch regulates pneumococcal virulence via global epigenetic changes
    Article Snippet: .. The PCR products were then digested with both DraI and PleI (New England Biolabs, MA, USA). .. This digestion was predicted to yield different sized FAM-labelled fragments for each of the variant forms.

    Article Title: Methylation Warfare: Interaction of Pneumococcal Bacteriophages with Their Host
    Article Snippet: .. A double digestion of the PCR products, using restriction enzymes DraI and PleI (New England Biolabs), allowed for the generation of labeled DNA fragments of different lengths, one specifically for each of the hsdS variant forms. ..

    Variant Assay:

    Article Title: Methylation Warfare: Interaction of Pneumococcal Bacteriophages with Their Host
    Article Snippet: .. A double digestion of the PCR products, using restriction enzymes DraI and PleI (New England Biolabs), allowed for the generation of labeled DNA fragments of different lengths, one specifically for each of the hsdS variant forms. ..

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96
    New England Biolabs ple i
    RFLP analysis of the gpd amplicons from various pathogenic treponeme strains. The gpd open reading frame was amplified from each of the specified strains, digested with <t>Ple</t> I, and subjected to agarose gel electrophoresis and ethidium bromide staining. The left lane shows the 100-bp DNA ladder (New England Biolabs). Shown also is the undigested gpd amplicon from the Nichols strain. The sizes, in base pairs, of the DNA fragments generated by <t>Ple</t> I digestion of the gpd amplicons from the various strains are shown on the right.
    Ple I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ple i/product/New England Biolabs
    Average 96 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    ple i - by Bioz Stars, 2020-07
    96/100 stars
      Buy from Supplier

    Image Search Results


    RFLP analysis of the gpd amplicons from various pathogenic treponeme strains. The gpd open reading frame was amplified from each of the specified strains, digested with Ple I, and subjected to agarose gel electrophoresis and ethidium bromide staining. The left lane shows the 100-bp DNA ladder (New England Biolabs). Shown also is the undigested gpd amplicon from the Nichols strain. The sizes, in base pairs, of the DNA fragments generated by Ple I digestion of the gpd amplicons from the various strains are shown on the right.

    Journal: Infection and Immunity

    Article Title: Sequence Conservation of Glycerophosphodiester Phosphodiesterase among Treponema pallidum Strains

    doi:

    Figure Lengend Snippet: RFLP analysis of the gpd amplicons from various pathogenic treponeme strains. The gpd open reading frame was amplified from each of the specified strains, digested with Ple I, and subjected to agarose gel electrophoresis and ethidium bromide staining. The left lane shows the 100-bp DNA ladder (New England Biolabs). Shown also is the undigested gpd amplicon from the Nichols strain. The sizes, in base pairs, of the DNA fragments generated by Ple I digestion of the gpd amplicons from the various strains are shown on the right.

    Article Snippet: One microgram of each of the amplified templates was digested with Ple I (New England Biolabs, Beverly, Mass.) for 4 h at 37°C prior to electrophoresis on a 1.5% NuSieve (FMC BioProducts, Rockland, Maine) agarose gel.

    Techniques: Amplification, Agarose Gel Electrophoresis, Staining, Generated