alwi  (New England Biolabs)


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  • 94
    Name:
    AlwI
    Description:
    AlwI 2 500 units
    Catalog Number:
    r0513l
    Price:
    282
    Size:
    2 500 units
    Category:
    Restriction Enzymes
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    Structured Review

    New England Biolabs alwi
    AlwI
    AlwI 2 500 units
    https://www.bioz.com/result/alwi/product/New England Biolabs
    Average 94 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    alwi - by Bioz Stars, 2020-07
    94/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Cell type-specific profiling of protein-DNA interactions without cell isolation using Targeted DamID with next-generation sequencing
    Article Snippet: .. ▲ CRITICAL STEP A polymerase with hotstart is required to prevent primer dimer formation during PCR AlwI (NEB, cat. no. R0513S) RNase A (DNase-free) (Roche, cat. no. 11119915001) diluted to 12.5μg/ml Qubit assay tubes (Invitrogen, cat. no. ) Qubit dsDNA HS assay kit, (Invitrogen, cat. no. ) Agilent reagents for TapeStation: Genomic DNA ScreenTape(5067-5365) and reagents (5067-5366) Agilent reagents for Bioanalyzer:Agilent DNA 1000 Kit (5067-1504) Agencourt AMPure XP Beads (Beckman Coulter, cat. no. A63880) (Seramag SpeedBeads, 3 EDAC/PA5 (Fisher Scientific, cat. no. 12326433) prepared using the method of Rohland and Reich, 2012 with 20%(w/v) PEG-8000 are a cost-effective alternative to AMPure XP beads) Quick ligase (NEB, cat. no. M2200S) T4 DNA ligase (400,000U/ml) and 10x buffer (NEB, cat. no. M0202S) T4 DNA polymerase (NEB, cat. no. M0203S) Klenow Fragment (NEB, cat. no. M0210S) Klenow 3' to 5' exo- (NEB, cat. no. M0541S) T4 polynucleotide kinase (NEB, cat. no. M0201S) NEBNext High-Fidelity 2X PCR Master Mix (NEB, cat. no. M0541S) dNTPs (NEB, cat. no. N0446S) .. Temperature controlled heat block capable of heating to 95°C.

    Article Title: Effect of PON1 gene polymorphisms in Turkish patients with hepatocellular carcinoma
    Article Snippet: .. The following cycling conditions were used: 95 °C for 5 min, followed by 35 cycles of 94 °C for 60 s, 58 °C for 60 s (57 °C for L55M) and 72 °C for 60 s, with a final extension at 72 °C for 10 min. After confirmation of successful PCR amplification by 1.5% agarose gel electrophoresis, each PCR product was digested overnight with 5 U AlwI (for Q192R) and 5 U NlaIII (for L55M) restriction endonuclease enzymes at 37 °C (New England Biolabs Inc., Beverly, MA) and electrophoresed on 3% agarose gel containing 0.5 μg/mL ethidium bromide and visualized under UV illumination. ..

    Article Title: Membrane-bound GFP-labelled vectors for Targeted DamID allow simultaneous profiling of expression domains and DNA binding
    Article Snippet: .. Following amplification, 2µg DNA was sonicated in a Bioruptor Plus (Diagenode), DamID adaptors removed by AlwI digestion, and 500ng of the resulting fragments end-repaired with a mix of enzymes (T4 DNA ligase (NEB) + Klenow Fragment (NEB) + T4 polynucleotide kinase (NEB)), A-tailed with Klenow 3’ to 5’ exo- (NEB), ligated to Illumina Truseq LT adaptors using Quick Ligase enzyme (NEB) and amplified via PCR with NEBNext Hi-fidelity enzyme (NEB). .. The resulting next-generation sequencing libraries were sequenced on a HiSeq2500 and reads were processed with damidseq_pipeline [ ].

    Article Title: Paraoxonase 1 R/Q alleles are associated with differential accumulation of saturated versus 20:5n3 fatty acid in human adipose tissue
    Article Snippet: .. Approximately 2 μg of PCR product was digested with the appropriate restriction endonucleases at 37°C for 4 h. CYP1A1 was detected with MspI, PON1 L/M with NlaIII, and PON1 Q/R with AlwI (New England Biolabs). .. All PCR samples with an undigested or partial-digested result were submitted to redigestion with 20 units/reaction of enzyme and overnight incubation.

    Isolation:

    Article Title: Coordinated repressive chromatin-remodeling of Oct4 and Nanog genes in RA-induced differentiation of embryonic stem cells involves RIP140
    Article Snippet: .. Isolated nuclei from ESCs were digested with 100 U of SapI, AlwI, FokI, XbaI, PstI, EcoRI or StuI (New England Biolabs, Ipswich, MA, USA, www.neb.com ) for 30 min. .. The purified genomic DNA was re-digested with 100 U of AvaI (for Nanog gene) or HindIII (for Oct4 gene).

    Amplification:

    Article Title: Effect of PON1 gene polymorphisms in Turkish patients with hepatocellular carcinoma
    Article Snippet: .. The following cycling conditions were used: 95 °C for 5 min, followed by 35 cycles of 94 °C for 60 s, 58 °C for 60 s (57 °C for L55M) and 72 °C for 60 s, with a final extension at 72 °C for 10 min. After confirmation of successful PCR amplification by 1.5% agarose gel electrophoresis, each PCR product was digested overnight with 5 U AlwI (for Q192R) and 5 U NlaIII (for L55M) restriction endonuclease enzymes at 37 °C (New England Biolabs Inc., Beverly, MA) and electrophoresed on 3% agarose gel containing 0.5 μg/mL ethidium bromide and visualized under UV illumination. ..

    Article Title: Membrane-bound GFP-labelled vectors for Targeted DamID allow simultaneous profiling of expression domains and DNA binding
    Article Snippet: .. Following amplification, 2µg DNA was sonicated in a Bioruptor Plus (Diagenode), DamID adaptors removed by AlwI digestion, and 500ng of the resulting fragments end-repaired with a mix of enzymes (T4 DNA ligase (NEB) + Klenow Fragment (NEB) + T4 polynucleotide kinase (NEB)), A-tailed with Klenow 3’ to 5’ exo- (NEB), ligated to Illumina Truseq LT adaptors using Quick Ligase enzyme (NEB) and amplified via PCR with NEBNext Hi-fidelity enzyme (NEB). .. The resulting next-generation sequencing libraries were sequenced on a HiSeq2500 and reads were processed with damidseq_pipeline [ ].

    Agarose Gel Electrophoresis:

    Article Title: Effect of PON1 gene polymorphisms in Turkish patients with hepatocellular carcinoma
    Article Snippet: .. The following cycling conditions were used: 95 °C for 5 min, followed by 35 cycles of 94 °C for 60 s, 58 °C for 60 s (57 °C for L55M) and 72 °C for 60 s, with a final extension at 72 °C for 10 min. After confirmation of successful PCR amplification by 1.5% agarose gel electrophoresis, each PCR product was digested overnight with 5 U AlwI (for Q192R) and 5 U NlaIII (for L55M) restriction endonuclease enzymes at 37 °C (New England Biolabs Inc., Beverly, MA) and electrophoresed on 3% agarose gel containing 0.5 μg/mL ethidium bromide and visualized under UV illumination. ..

    Sonication:

    Article Title: Membrane-bound GFP-labelled vectors for Targeted DamID allow simultaneous profiling of expression domains and DNA binding
    Article Snippet: .. Following amplification, 2µg DNA was sonicated in a Bioruptor Plus (Diagenode), DamID adaptors removed by AlwI digestion, and 500ng of the resulting fragments end-repaired with a mix of enzymes (T4 DNA ligase (NEB) + Klenow Fragment (NEB) + T4 polynucleotide kinase (NEB)), A-tailed with Klenow 3’ to 5’ exo- (NEB), ligated to Illumina Truseq LT adaptors using Quick Ligase enzyme (NEB) and amplified via PCR with NEBNext Hi-fidelity enzyme (NEB). .. The resulting next-generation sequencing libraries were sequenced on a HiSeq2500 and reads were processed with damidseq_pipeline [ ].

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  • 94
    New England Biolabs alwi
    Alwi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alwi/product/New England Biolabs
    Average 94 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    alwi - by Bioz Stars, 2020-07
    94/100 stars
      Buy from Supplier

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