alwi  (New England Biolabs)


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  • 94
    Name:
    AlwI
    Description:
    AlwI 2 500 units
    Catalog Number:
    R0513L
    Price:
    282
    Category:
    Restriction Enzymes
    Size:
    2 500 units
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    Structured Review

    New England Biolabs alwi
    AlwI
    AlwI 2 500 units
    https://www.bioz.com/result/alwi/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    alwi - by Bioz Stars, 2021-06
    94/100 stars

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    Related Articles

    Amplification:

    Article Title: FlyORF-TaDa allows rapid generation of new lines for in vivo cell-type specific profiling of protein-DNA interactions in Drosophila melanogaster
    Article Snippet: Following amplification, 2 µg DNA was sonicated in a Bioruptor Plus (Diagenode). .. DamID adaptors were removed by AlwI digestion, and 500 ng of the resulting fragments end-repaired with a mix of enzymes (T4 DNA ligase (NEB) + Klenow Fragment (NEB) + T4 polynucleotide kinase (NEB)), A-tailed with Klenow 3’ to 5’ exo-(NEB), ligated to Illumina Truseq LT adaptors using Quick Ligase enzyme (NEB) and amplified via PCR with NEBNext Hi-fidelity enzyme (NEB). .. The resulting next-generation sequencing libraries were sequenced on a HiSeq2500 (Illumina).

    Article Title: Membrane-bound GFP-labelled vectors for Targeted DamID allow simultaneous profiling of expression domains and DNA binding
    Article Snippet: Briefly, DNA was extracted using a Quick-DNA Miniprep plus kit (Zymo), digested with DpnI (NEB) overnight and cleaned-up with a PCR purification kit (Machery-Nagel), DamID adaptors were ligated, digested with DpnII (NEB) for 2 hours, and amplified via PCR using MyTaq DNA polymerase (Bioline). .. Following amplification, 2µg DNA was sonicated in a Bioruptor Plus (Diagenode), DamID adaptors removed by AlwI digestion, and 500ng of the resulting fragments end-repaired with a mix of enzymes (T4 DNA ligase (NEB) + Klenow Fragment (NEB) + T4 polynucleotide kinase (NEB)), A-tailed with Klenow 3’ to 5’ exo- (NEB), ligated to Illumina Truseq LT adaptors using Quick Ligase enzyme (NEB) and amplified via PCR with NEBNext Hi-fidelity enzyme (NEB). .. The resulting next-generation sequencing libraries were sequenced on a HiSeq2500 and reads were processed with damidseq_pipeline [ ].

    Article Title: Effect of PON1 gene polymorphisms in Turkish patients with hepatocellular carcinoma
    Article Snippet: The 20 μL PCR mixture contained approximately 250 ng DNA, with 0.25 μM of both primers, 0.1 mM of each dNTP, 1 × PCR buffer, 1.5 mM MgCI2 and 1 U Taq polymerase (Promega, Madison, WI, USA). .. The following cycling conditions were used: 95 °C for 5 min, followed by 35 cycles of 94 °C for 60 s, 58 °C for 60 s (57 °C for L55M) and 72 °C for 60 s, with a final extension at 72 °C for 10 min. After confirmation of successful PCR amplification by 1.5% agarose gel electrophoresis, each PCR product was digested overnight with 5 U AlwI (for Q192R) and 5 U NlaIII (for L55M) restriction endonuclease enzymes at 37 °C (New England Biolabs Inc., Beverly, MA) and electrophoresed on 3% agarose gel containing 0.5 μg/mL ethidium bromide and visualized under UV illumination. ..

    Article Title: FlyORF-TaDa allows rapid generation of new lines for in vivo cell-type-specific profiling of protein–DNA interactions in Drosophila melanogaster
    Article Snippet: Following amplification, 2 µg DNA was sonicated in a Bioruptor Plus (Diagenode). .. DamID adaptors were removed by AlwI digestion, and 500 ng of the resulting fragments end-repaired with a mix of enzymes [T4 DNA ligase (NEB) + Klenow Fragment (NEB) + T4 polynucleotide kinase (NEB)], A-tailed with Klenow 3′→5′ exo- (NEB), ligated to Illumina Truseq LT adaptors using Quick Ligase enzyme (NEB), and amplified via PCR with NEBNext Hi-fidelity enzyme (NEB). .. The resulting next-generation sequencing libraries were sequenced on a HiSeq 2500 (Illumina).

    Polymerase Chain Reaction:

    Article Title: FlyORF-TaDa allows rapid generation of new lines for in vivo cell-type specific profiling of protein-DNA interactions in Drosophila melanogaster
    Article Snippet: Following amplification, 2 µg DNA was sonicated in a Bioruptor Plus (Diagenode). .. DamID adaptors were removed by AlwI digestion, and 500 ng of the resulting fragments end-repaired with a mix of enzymes (T4 DNA ligase (NEB) + Klenow Fragment (NEB) + T4 polynucleotide kinase (NEB)), A-tailed with Klenow 3’ to 5’ exo-(NEB), ligated to Illumina Truseq LT adaptors using Quick Ligase enzyme (NEB) and amplified via PCR with NEBNext Hi-fidelity enzyme (NEB). .. The resulting next-generation sequencing libraries were sequenced on a HiSeq2500 (Illumina).

    Article Title: Paraoxonase 1 R/Q alleles are associated with differential accumulation of saturated versus 20:5n3 fatty acid in human adipose tissue
    Article Snippet: The reaction mix consisted of PCR buffer, 1.5 mM MgCl2 , 0.2 mM of each dNTP, 0.5 mM of each primer, and 1.2 units/reaction Taq DNA polymerase (Invitrogen). .. Approximately 2 μg of PCR product was digested with the appropriate restriction endonucleases at 37°C for 4 h. CYP1A1 was detected with MspI, PON1 L/M with NlaIII, and PON1 Q/R with AlwI (New England Biolabs). .. All PCR samples with an undigested or partial-digested result were submitted to redigestion with 20 units/reaction of enzyme and overnight incubation.

    Article Title: Membrane-bound GFP-labelled vectors for Targeted DamID allow simultaneous profiling of expression domains and DNA binding
    Article Snippet: Briefly, DNA was extracted using a Quick-DNA Miniprep plus kit (Zymo), digested with DpnI (NEB) overnight and cleaned-up with a PCR purification kit (Machery-Nagel), DamID adaptors were ligated, digested with DpnII (NEB) for 2 hours, and amplified via PCR using MyTaq DNA polymerase (Bioline). .. Following amplification, 2µg DNA was sonicated in a Bioruptor Plus (Diagenode), DamID adaptors removed by AlwI digestion, and 500ng of the resulting fragments end-repaired with a mix of enzymes (T4 DNA ligase (NEB) + Klenow Fragment (NEB) + T4 polynucleotide kinase (NEB)), A-tailed with Klenow 3’ to 5’ exo- (NEB), ligated to Illumina Truseq LT adaptors using Quick Ligase enzyme (NEB) and amplified via PCR with NEBNext Hi-fidelity enzyme (NEB). .. The resulting next-generation sequencing libraries were sequenced on a HiSeq2500 and reads were processed with damidseq_pipeline [ ].

    Article Title: Effect of PON1 gene polymorphisms in Turkish patients with hepatocellular carcinoma
    Article Snippet: The 20 μL PCR mixture contained approximately 250 ng DNA, with 0.25 μM of both primers, 0.1 mM of each dNTP, 1 × PCR buffer, 1.5 mM MgCI2 and 1 U Taq polymerase (Promega, Madison, WI, USA). .. The following cycling conditions were used: 95 °C for 5 min, followed by 35 cycles of 94 °C for 60 s, 58 °C for 60 s (57 °C for L55M) and 72 °C for 60 s, with a final extension at 72 °C for 10 min. After confirmation of successful PCR amplification by 1.5% agarose gel electrophoresis, each PCR product was digested overnight with 5 U AlwI (for Q192R) and 5 U NlaIII (for L55M) restriction endonuclease enzymes at 37 °C (New England Biolabs Inc., Beverly, MA) and electrophoresed on 3% agarose gel containing 0.5 μg/mL ethidium bromide and visualized under UV illumination. ..

    Article Title: FlyORF-TaDa allows rapid generation of new lines for in vivo cell-type-specific profiling of protein–DNA interactions in Drosophila melanogaster
    Article Snippet: Following amplification, 2 µg DNA was sonicated in a Bioruptor Plus (Diagenode). .. DamID adaptors were removed by AlwI digestion, and 500 ng of the resulting fragments end-repaired with a mix of enzymes [T4 DNA ligase (NEB) + Klenow Fragment (NEB) + T4 polynucleotide kinase (NEB)], A-tailed with Klenow 3′→5′ exo- (NEB), ligated to Illumina Truseq LT adaptors using Quick Ligase enzyme (NEB), and amplified via PCR with NEBNext Hi-fidelity enzyme (NEB). .. The resulting next-generation sequencing libraries were sequenced on a HiSeq 2500 (Illumina).

    Sonication:

    Article Title: Membrane-bound GFP-labelled vectors for Targeted DamID allow simultaneous profiling of expression domains and DNA binding
    Article Snippet: Briefly, DNA was extracted using a Quick-DNA Miniprep plus kit (Zymo), digested with DpnI (NEB) overnight and cleaned-up with a PCR purification kit (Machery-Nagel), DamID adaptors were ligated, digested with DpnII (NEB) for 2 hours, and amplified via PCR using MyTaq DNA polymerase (Bioline). .. Following amplification, 2µg DNA was sonicated in a Bioruptor Plus (Diagenode), DamID adaptors removed by AlwI digestion, and 500ng of the resulting fragments end-repaired with a mix of enzymes (T4 DNA ligase (NEB) + Klenow Fragment (NEB) + T4 polynucleotide kinase (NEB)), A-tailed with Klenow 3’ to 5’ exo- (NEB), ligated to Illumina Truseq LT adaptors using Quick Ligase enzyme (NEB) and amplified via PCR with NEBNext Hi-fidelity enzyme (NEB). .. The resulting next-generation sequencing libraries were sequenced on a HiSeq2500 and reads were processed with damidseq_pipeline [ ].

    Article Title: Novel CHD8 genomic targets identified in fetal mouse brain by in vivo Targeted DamID
    Article Snippet: PCR amplification of DpnII-digested fragments using MyTaq (Bioline, BIO-21112) enriched for methylated fragments before samples were sonicated and prepped for sequencing. .. Sonicated samples were subjected to AlwI digestion (NEB, R0513S) to remove previously ligated adaptors and initial GATC sequences from fragments. .. A modified TruSeq protocol was used to generate sequencing libraries involving end repair, 3’ end adenylation, sequencing adaptor ligation, and DNA fragment enrichment using a reduced number of PCR cycles.

    Agarose Gel Electrophoresis:

    Article Title: Effect of PON1 gene polymorphisms in Turkish patients with hepatocellular carcinoma
    Article Snippet: The 20 μL PCR mixture contained approximately 250 ng DNA, with 0.25 μM of both primers, 0.1 mM of each dNTP, 1 × PCR buffer, 1.5 mM MgCI2 and 1 U Taq polymerase (Promega, Madison, WI, USA). .. The following cycling conditions were used: 95 °C for 5 min, followed by 35 cycles of 94 °C for 60 s, 58 °C for 60 s (57 °C for L55M) and 72 °C for 60 s, with a final extension at 72 °C for 10 min. After confirmation of successful PCR amplification by 1.5% agarose gel electrophoresis, each PCR product was digested overnight with 5 U AlwI (for Q192R) and 5 U NlaIII (for L55M) restriction endonuclease enzymes at 37 °C (New England Biolabs Inc., Beverly, MA) and electrophoresed on 3% agarose gel containing 0.5 μg/mL ethidium bromide and visualized under UV illumination. ..

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  • 94
    New England Biolabs alwi digestion
    Alwi Digestion, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alwi digestion/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    alwi digestion - by Bioz Stars, 2021-06
    94/100 stars
      Buy from Supplier

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