eaei restriction enzyme  (New England Biolabs)


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    Structured Review

    New England Biolabs eaei restriction enzyme
    Eaei Restriction Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/eaei restriction enzyme/product/New England Biolabs
    Average 85 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    eaei restriction enzyme - by Bioz Stars, 2020-03
    85/100 stars

    Related Products / Commonly Used Together

    pcr
    dgat1 k232a

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    Amplification:

    Article Title: The Tumor Suppressor Chromodomain Helicase DNA-binding Protein 5 (CHD5) Remodels Nucleosomes by Unwrapping *
    Article Snippet: A 157-bp EcoRI-RsaI fragment containing a Xenopus 5S rDNA sequence was PCR amplified from the plasmid pXP-10 ( ). .. Cac8I (5 units/ml), EaeI (1.5 units/ml) or EcoRV (10 units/ml) (New England Biolabs) was added, and the reactions incubated at 37 °C for an additional 1 h. ATP or UTP (3 m m final) was included for the Cac8I and EaeI digestions.

    Article Title: Milk fatty acid variability and association with polymorphisms in SCD1 and DGAT1 genes in White Fulani and Borgou cattle breeds
    Article Snippet: Amplification was confirmed by running the PCR products on 1.8% agarose gel and visualized with GelDoc-It2 Imager (Ultra-Violet Products Ltd., UK). .. Five microliter of purified PCR products containing the DGAT1 K232A were digested overnight at 37 °C with 10 U of EaeI restriction enzyme (New England Biolabs, Inc., USA).

    Article Title: Milk fatty acid variability and association with polymorphisms in SCD1 and DGAT1 genes in White Fulani and Borgou cattle breeds
    Article Snippet: Amplification was confirmed by running the PCR products on 1.8% agarose gel and visualized with GelDoc-It2 Imager (Ultra-Violet Products Ltd., UK). .. Five microliter of purified PCR products containing the DGAT1 K232A were digested overnight at 37 °C with 10 U of EaeI restriction enzyme (New England Biolabs, Inc., USA).

    Agarose Gel Electrophoresis:

    Article Title: Milk fatty acid variability and association with polymorphisms in SCD1 and DGAT1 genes in White Fulani and Borgou cattle breeds
    Article Snippet: Amplification was confirmed by running the PCR products on 1.8% agarose gel and visualized with GelDoc-It2 Imager (Ultra-Violet Products Ltd., UK). .. Five microliter of purified PCR products containing the DGAT1 K232A were digested overnight at 37 °C with 10 U of EaeI restriction enzyme (New England Biolabs, Inc., USA).

    Article Title: Milk fatty acid variability and association with polymorphisms in SCD1 and DGAT1 genes in White Fulani and Borgou cattle breeds
    Article Snippet: Amplification was confirmed by running the PCR products on 1.8% agarose gel and visualized with GelDoc-It2 Imager (Ultra-Violet Products Ltd., UK). .. Five microliter of purified PCR products containing the DGAT1 K232A were digested overnight at 37 °C with 10 U of EaeI restriction enzyme (New England Biolabs, Inc., USA).

    Mutagenesis:

    Article Title: Milk fatty acid variability and association with polymorphisms in SCD1 and DGAT1 genes in White Fulani and Borgou cattle breeds
    Article Snippet: Five microliter of purified PCR products containing the DGAT1 K232A were digested overnight at 37 °C with 10 U of EaeI restriction enzyme (New England Biolabs, Inc., USA). .. Similarly, 5 µl of purified PCR products containing the SCD1 A293V mutation were digested overnight at 37 °C with 10 U of Nco I restriction enzyme (New England Biolabs, Inc., USA).

    Article Title: Milk fatty acid variability and association with polymorphisms in SCD1 and DGAT1 genes in White Fulani and Borgou cattle breeds
    Article Snippet: Five microliter of purified PCR products containing the DGAT1 K232A were digested overnight at 37 °C with 10 U of EaeI restriction enzyme (New England Biolabs, Inc., USA). .. Similarly, 5 µl of purified PCR products containing the SCD1 A293V mutation were digested overnight at 37 °C with 10 U of Nco I restriction enzyme (New England Biolabs, Inc., USA).

    Labeling:

    Article Title: Epoxyalkane:Coenzyme M Transferase Gene Diversity and Distribution in Groundwater Samples from Chlorinated-Ethene-Contaminated Sites
    Article Snippet: The restriction enzyme AcoI (EaeI) (New England BioLabs, Inc., Ipswich, MA) was selected for EaCoMT T-RFLP analysis using the default settings of the software program REPK ( ). .. All samples used in T-RFLP analysis were subjected to nested PCR with fluorescently labeled 6-carboxyfluorescein (6-FAM) F131 and unlabeled R562 primers.

    Purification:

    Article Title: The Tumor Suppressor Chromodomain Helicase DNA-binding Protein 5 (CHD5) Remodels Nucleosomes by Unwrapping *
    Article Snippet: The fragment was gel purified and assembled into mononucleosomes by salt dialysis, briefly digested with MNase, and purified on a glycerol gradient. .. Cac8I (5 units/ml), EaeI (1.5 units/ml) or EcoRV (10 units/ml) (New England Biolabs) was added, and the reactions incubated at 37 °C for an additional 1 h. ATP or UTP (3 m m final) was included for the Cac8I and EaeI digestions.

    Article Title: Milk fatty acid variability and association with polymorphisms in SCD1 and DGAT1 genes in White Fulani and Borgou cattle breeds
    Article Snippet: .. Five microliter of purified PCR products containing the DGAT1 K232A were digested overnight at 37 °C with 10 U of EaeI restriction enzyme (New England Biolabs, Inc., USA). .. The digested PCR products were separated on 1.8% agarose gel stained with GelRed™ (Biotium, UK) resulting in two fragments of 203 and 208 bp (seen as a single band on gel) for AA genotype, two fragments of 203/208 bp and 411 bp for KA genotype and the undigested 411 bp for KK genotype (Additional file 1: Figure a).

    Article Title: Milk fatty acid variability and association with polymorphisms in SCD1 and DGAT1 genes in White Fulani and Borgou cattle breeds
    Article Snippet: .. Five microliter of purified PCR products containing the DGAT1 K232A were digested overnight at 37 °C with 10 U of EaeI restriction enzyme (New England Biolabs, Inc., USA). .. The digested PCR products were separated on 1.8% agarose gel stained with GelRed™ (Biotium, UK) resulting in two fragments of 203 and 208 bp (seen as a single band on gel) for AA genotype, two fragments of 203/208 bp and 411 bp for KA genotype and the undigested 411 bp for KK genotype (Additional file 1: Figure a).

    SYBR Green Assay:

    Article Title: The Tumor Suppressor Chromodomain Helicase DNA-binding Protein 5 (CHD5) Remodels Nucleosomes by Unwrapping *
    Article Snippet: Cac8I (5 units/ml), EaeI (1.5 units/ml) or EcoRV (10 units/ml) (New England Biolabs) was added, and the reactions incubated at 37 °C for an additional 1 h. ATP or UTP (3 m m final) was included for the Cac8I and EaeI digestions. .. DNA fragments were resolved by PAGE and visualized by SYBR green staining.

    Polymerase Chain Reaction:

    Article Title: Epoxyalkane:Coenzyme M Transferase Gene Diversity and Distribution in Groundwater Samples from Chlorinated-Ethene-Contaminated Sites
    Article Snippet: The restriction enzyme AcoI (EaeI) (New England BioLabs, Inc., Ipswich, MA) was selected for EaCoMT T-RFLP analysis using the default settings of the software program REPK ( ). .. PCR products (12 μl) were digested with AcoI (EaeI) and precipitated with glycogen, sodium acetate, and ethanol in accordance with the manufacturer's protocol.

    Article Title: The Tumor Suppressor Chromodomain Helicase DNA-binding Protein 5 (CHD5) Remodels Nucleosomes by Unwrapping *
    Article Snippet: A 157-bp EcoRI-RsaI fragment containing a Xenopus 5S rDNA sequence was PCR amplified from the plasmid pXP-10 ( ). .. Cac8I (5 units/ml), EaeI (1.5 units/ml) or EcoRV (10 units/ml) (New England Biolabs) was added, and the reactions incubated at 37 °C for an additional 1 h. ATP or UTP (3 m m final) was included for the Cac8I and EaeI digestions.

    Article Title: Milk fatty acid variability and association with polymorphisms in SCD1 and DGAT1 genes in White Fulani and Borgou cattle breeds
    Article Snippet: .. Five microliter of purified PCR products containing the DGAT1 K232A were digested overnight at 37 °C with 10 U of EaeI restriction enzyme (New England Biolabs, Inc., USA). .. The digested PCR products were separated on 1.8% agarose gel stained with GelRed™ (Biotium, UK) resulting in two fragments of 203 and 208 bp (seen as a single band on gel) for AA genotype, two fragments of 203/208 bp and 411 bp for KA genotype and the undigested 411 bp for KK genotype (Additional file 1: Figure a).

    Article Title: Milk fatty acid variability and association with polymorphisms in SCD1 and DGAT1 genes in White Fulani and Borgou cattle breeds
    Article Snippet: .. Five microliter of purified PCR products containing the DGAT1 K232A were digested overnight at 37 °C with 10 U of EaeI restriction enzyme (New England Biolabs, Inc., USA). .. The digested PCR products were separated on 1.8% agarose gel stained with GelRed™ (Biotium, UK) resulting in two fragments of 203 and 208 bp (seen as a single band on gel) for AA genotype, two fragments of 203/208 bp and 411 bp for KA genotype and the undigested 411 bp for KK genotype (Additional file 1: Figure a).

    Incubation:

    Article Title: The Tumor Suppressor Chromodomain Helicase DNA-binding Protein 5 (CHD5) Remodels Nucleosomes by Unwrapping *
    Article Snippet: .. Cac8I (5 units/ml), EaeI (1.5 units/ml) or EcoRV (10 units/ml) (New England Biolabs) was added, and the reactions incubated at 37 °C for an additional 1 h. ATP or UTP (3 m m final) was included for the Cac8I and EaeI digestions. ..

    Terminal Restriction Fragment Length Polymorphism:

    Article Title: Epoxyalkane:Coenzyme M Transferase Gene Diversity and Distribution in Groundwater Samples from Chlorinated-Ethene-Contaminated Sites
    Article Snippet: .. The restriction enzyme AcoI (EaeI) (New England BioLabs, Inc., Ipswich, MA) was selected for EaCoMT T-RFLP analysis using the default settings of the software program REPK ( ). .. This restriction enzyme maximized differentiation between EaCoMT sequences, based on analysis of a database populated with full-length EaCoMT sequences from VC- and ethene-assimilating isolates deposited in GenBank (see Table S5 in the supplemental material).

    Polyacrylamide Gel Electrophoresis:

    Article Title: The Tumor Suppressor Chromodomain Helicase DNA-binding Protein 5 (CHD5) Remodels Nucleosomes by Unwrapping *
    Article Snippet: Cac8I (5 units/ml), EaeI (1.5 units/ml) or EcoRV (10 units/ml) (New England Biolabs) was added, and the reactions incubated at 37 °C for an additional 1 h. ATP or UTP (3 m m final) was included for the Cac8I and EaeI digestions. .. DNA fragments were resolved by PAGE and visualized by SYBR green staining.

    Sequencing:

    Article Title: The Tumor Suppressor Chromodomain Helicase DNA-binding Protein 5 (CHD5) Remodels Nucleosomes by Unwrapping *
    Article Snippet: A 157-bp EcoRI-RsaI fragment containing a Xenopus 5S rDNA sequence was PCR amplified from the plasmid pXP-10 ( ). .. Cac8I (5 units/ml), EaeI (1.5 units/ml) or EcoRV (10 units/ml) (New England Biolabs) was added, and the reactions incubated at 37 °C for an additional 1 h. ATP or UTP (3 m m final) was included for the Cac8I and EaeI digestions.

    Staining:

    Article Title: The Tumor Suppressor Chromodomain Helicase DNA-binding Protein 5 (CHD5) Remodels Nucleosomes by Unwrapping *
    Article Snippet: Cac8I (5 units/ml), EaeI (1.5 units/ml) or EcoRV (10 units/ml) (New England Biolabs) was added, and the reactions incubated at 37 °C for an additional 1 h. ATP or UTP (3 m m final) was included for the Cac8I and EaeI digestions. .. DNA fragments were resolved by PAGE and visualized by SYBR green staining.

    Article Title: Milk fatty acid variability and association with polymorphisms in SCD1 and DGAT1 genes in White Fulani and Borgou cattle breeds
    Article Snippet: Five microliter of purified PCR products containing the DGAT1 K232A were digested overnight at 37 °C with 10 U of EaeI restriction enzyme (New England Biolabs, Inc., USA). .. The digested PCR products were separated on 1.8% agarose gel stained with GelRed™ (Biotium, UK) resulting in two fragments of 203 and 208 bp (seen as a single band on gel) for AA genotype, two fragments of 203/208 bp and 411 bp for KA genotype and the undigested 411 bp for KK genotype (Additional file 1: Figure a).

    Article Title: Milk fatty acid variability and association with polymorphisms in SCD1 and DGAT1 genes in White Fulani and Borgou cattle breeds
    Article Snippet: Five microliter of purified PCR products containing the DGAT1 K232A were digested overnight at 37 °C with 10 U of EaeI restriction enzyme (New England Biolabs, Inc., USA). .. The digested PCR products were separated on 1.8% agarose gel stained with GelRed™ (Biotium, UK) resulting in two fragments of 203 and 208 bp (seen as a single band on gel) for AA genotype, two fragments of 203/208 bp and 411 bp for KA genotype and the undigested 411 bp for KK genotype (Additional file 1: Figure a).

    Nested PCR:

    Article Title: Epoxyalkane:Coenzyme M Transferase Gene Diversity and Distribution in Groundwater Samples from Chlorinated-Ethene-Contaminated Sites
    Article Snippet: The restriction enzyme AcoI (EaeI) (New England BioLabs, Inc., Ipswich, MA) was selected for EaCoMT T-RFLP analysis using the default settings of the software program REPK ( ). .. All samples used in T-RFLP analysis were subjected to nested PCR with fluorescently labeled 6-carboxyfluorescein (6-FAM) F131 and unlabeled R562 primers.

    Plasmid Preparation:

    Article Title: The Tumor Suppressor Chromodomain Helicase DNA-binding Protein 5 (CHD5) Remodels Nucleosomes by Unwrapping *
    Article Snippet: A 157-bp EcoRI-RsaI fragment containing a Xenopus 5S rDNA sequence was PCR amplified from the plasmid pXP-10 ( ). .. Cac8I (5 units/ml), EaeI (1.5 units/ml) or EcoRV (10 units/ml) (New England Biolabs) was added, and the reactions incubated at 37 °C for an additional 1 h. ATP or UTP (3 m m final) was included for the Cac8I and EaeI digestions.

    Software:

    Article Title: Epoxyalkane:Coenzyme M Transferase Gene Diversity and Distribution in Groundwater Samples from Chlorinated-Ethene-Contaminated Sites
    Article Snippet: .. The restriction enzyme AcoI (EaeI) (New England BioLabs, Inc., Ipswich, MA) was selected for EaCoMT T-RFLP analysis using the default settings of the software program REPK ( ). .. This restriction enzyme maximized differentiation between EaCoMT sequences, based on analysis of a database populated with full-length EaCoMT sequences from VC- and ethene-assimilating isolates deposited in GenBank (see Table S5 in the supplemental material).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 85
    New England Biolabs eaei restriction enzyme
    Eaei Restriction Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/eaei restriction enzyme/product/New England Biolabs
    Average 85 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    eaei restriction enzyme - by Bioz Stars, 2020-03
    85/100 stars
      Buy from Supplier

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