bspmi  (New England Biolabs)


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  • 93
    Name:
    BspMI
    Description:

    Catalog Number:
    R0502
    Price:
    69
    Category:
    Restriction Enzymes
    Applications:
    DNA Manipulation
    Size:
    100 units
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    Structured Review

    New England Biolabs bspmi
    BspMI

    https://www.bioz.com/result/bspmi/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bspmi - by Bioz Stars, 2021-07
    93/100 stars

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    Related Articles

    Construct:

    Article Title: Anti-Candidal Activity and Functional Mapping of Recombinant and Synthetic Neosartorya fischeri Antifungal Protein 2 (NFAP2)
    Article Snippet: .. The paf 5′- and 3′-UTR flanking construct was amplified (Q5 High Fidelity DNA Polymerase, New England Biolabs, Ipswich, MA, USA) from this vector using the primer pair 1stF (5′- GGC CCC ATG GTG GTA AAC AAG TAG TG−3′) / 1stR (5′- GGT GGC GGC CGC TCT AGA ACT AG−3′), and after digestion with BspM I, Not I (New England Biolabs, Ipswich, MA, USA) the resulted 1033 bp amplicon was inserted into the respectively digested pSK275paf vector (Sonderegger et al., ) resulting in the expression vector pSK275nfap2 . ..

    Amplification:

    Article Title: Anti-Candidal Activity and Functional Mapping of Recombinant and Synthetic Neosartorya fischeri Antifungal Protein 2 (NFAP2)
    Article Snippet: .. The paf 5′- and 3′-UTR flanking construct was amplified (Q5 High Fidelity DNA Polymerase, New England Biolabs, Ipswich, MA, USA) from this vector using the primer pair 1stF (5′- GGC CCC ATG GTG GTA AAC AAG TAG TG−3′) / 1stR (5′- GGT GGC GGC CGC TCT AGA ACT AG−3′), and after digestion with BspM I, Not I (New England Biolabs, Ipswich, MA, USA) the resulted 1033 bp amplicon was inserted into the respectively digested pSK275paf vector (Sonderegger et al., ) resulting in the expression vector pSK275nfap2 . ..

    Plasmid Preparation:

    Article Title: Anti-Candidal Activity and Functional Mapping of Recombinant and Synthetic Neosartorya fischeri Antifungal Protein 2 (NFAP2)
    Article Snippet: .. The paf 5′- and 3′-UTR flanking construct was amplified (Q5 High Fidelity DNA Polymerase, New England Biolabs, Ipswich, MA, USA) from this vector using the primer pair 1stF (5′- GGC CCC ATG GTG GTA AAC AAG TAG TG−3′) / 1stR (5′- GGT GGC GGC CGC TCT AGA ACT AG−3′), and after digestion with BspM I, Not I (New England Biolabs, Ipswich, MA, USA) the resulted 1033 bp amplicon was inserted into the respectively digested pSK275paf vector (Sonderegger et al., ) resulting in the expression vector pSK275nfap2 . ..

    Article Title: Bacillus anthracis Virulence Regulator AtxA Binds Specifically to the pagA Promoter Region
    Article Snippet: Error-prone PCR used 1 μl each of 100 μM SwaI F and 100 μM BspMI R primers and 100 ng of pKA2-AtxA H379D linearized by digestion with EcoRI-HF (New England BioLabs) in a 100-μl reaction mixture. .. The modified gene was then cut out with SwaI and BspMI enzymes (New England BioLabs) and ligated into appropriately digested pKA2 plasmid. .. The mutated pKA2 plasmid pool was transformed into E. coli One Shot TOP10 competent cells (Invitrogen Corporation), following the manufacturer’s protocol, and then into E. coli SCS110 competent cells (Agilent Technologies).

    Countercurrent Chromatography:

    Article Title: Anti-Candidal Activity and Functional Mapping of Recombinant and Synthetic Neosartorya fischeri Antifungal Protein 2 (NFAP2)
    Article Snippet: .. The paf 5′- and 3′-UTR flanking construct was amplified (Q5 High Fidelity DNA Polymerase, New England Biolabs, Ipswich, MA, USA) from this vector using the primer pair 1stF (5′- GGC CCC ATG GTG GTA AAC AAG TAG TG−3′) / 1stR (5′- GGT GGC GGC CGC TCT AGA ACT AG−3′), and after digestion with BspM I, Not I (New England Biolabs, Ipswich, MA, USA) the resulted 1033 bp amplicon was inserted into the respectively digested pSK275paf vector (Sonderegger et al., ) resulting in the expression vector pSK275nfap2 . ..

    Activated Clotting Time Assay:

    Article Title: Anti-Candidal Activity and Functional Mapping of Recombinant and Synthetic Neosartorya fischeri Antifungal Protein 2 (NFAP2)
    Article Snippet: .. The paf 5′- and 3′-UTR flanking construct was amplified (Q5 High Fidelity DNA Polymerase, New England Biolabs, Ipswich, MA, USA) from this vector using the primer pair 1stF (5′- GGC CCC ATG GTG GTA AAC AAG TAG TG−3′) / 1stR (5′- GGT GGC GGC CGC TCT AGA ACT AG−3′), and after digestion with BspM I, Not I (New England Biolabs, Ipswich, MA, USA) the resulted 1033 bp amplicon was inserted into the respectively digested pSK275paf vector (Sonderegger et al., ) resulting in the expression vector pSK275nfap2 . ..

    Expressing:

    Article Title: Anti-Candidal Activity and Functional Mapping of Recombinant and Synthetic Neosartorya fischeri Antifungal Protein 2 (NFAP2)
    Article Snippet: .. The paf 5′- and 3′-UTR flanking construct was amplified (Q5 High Fidelity DNA Polymerase, New England Biolabs, Ipswich, MA, USA) from this vector using the primer pair 1stF (5′- GGC CCC ATG GTG GTA AAC AAG TAG TG−3′) / 1stR (5′- GGT GGC GGC CGC TCT AGA ACT AG−3′), and after digestion with BspM I, Not I (New England Biolabs, Ipswich, MA, USA) the resulted 1033 bp amplicon was inserted into the respectively digested pSK275paf vector (Sonderegger et al., ) resulting in the expression vector pSK275nfap2 . ..

    Modification:

    Article Title: Bacillus anthracis Virulence Regulator AtxA Binds Specifically to the pagA Promoter Region
    Article Snippet: Error-prone PCR used 1 μl each of 100 μM SwaI F and 100 μM BspMI R primers and 100 ng of pKA2-AtxA H379D linearized by digestion with EcoRI-HF (New England BioLabs) in a 100-μl reaction mixture. .. The modified gene was then cut out with SwaI and BspMI enzymes (New England BioLabs) and ligated into appropriately digested pKA2 plasmid. .. The mutated pKA2 plasmid pool was transformed into E. coli One Shot TOP10 competent cells (Invitrogen Corporation), following the manufacturer’s protocol, and then into E. coli SCS110 competent cells (Agilent Technologies).

    Agarose Gel Electrophoresis:

    Article Title: Zebrafish bmp4 functions during late gastrulation to specify ventroposterior cell fates
    Article Snippet: Genomic DNA was isolated from pools of 40 st37 / st37 , st37 /+, and +/+ embryos at 5dpf with a Qiagen DNeasy kit. .. 10 ug of the genomic DNA was digested with BstXI, PsiI, HpyCHIV, NheI, SmlI, SfcI, MmeI, BspMI, or BssSI (NEB), run on a 1% agarose gel, and then transferred to a charged nylon membrane (Hybond-N+, Amersham Biosciences). .. Probes were amplified from genomic DNA (5′ probe: 5′-CCGCTGATAAATAAGAGACTAAGC-3′ and 5′-GCTTTAAGCATAATCTGAAATCACTC-3′, 667bp; 3′probe: 5′-ACACCGTGCGCATCTCTT-3′ and 5′-GGCTGCTAATATGATTTCCCTGT-3′, 579bp) and radioactively labeled with the Rediprime II Random Prime Labelling System (Amersham Biosciences).

    other:

    Article Title: DNA looping by two-site restriction endonucleases: heterogeneous probability distributions for loop size and unbinding force
    Article Snippet: Endonucleases BamHI, BpmI, BsgI, BspMI, BstNI, EcoRI, EcoRV, FokI, HaeIII, HpaII, MboII, MspI, NarI, NaeI, SacII, Sau3AI, SfiI and SgrAI were obtained from New England Biolabs (NEB).

    Incubation:

    Article Title: Bending and looping of long DNA by Polycomb repressive complex 2 revealed by AFM imaging in liquid
    Article Snippet: The correct length band was excised and then extracted (Bio-Rad 7326165), concentrated (Millipore UFC501024), and purified a final time (Qiaquick). .. Experiments then proceeded as described elsewhere , except incubation occurred with 100 U/ml of BspMI (New England Biolabs, R0502S) in the buffer, instead of PRC2; MgCl2 was replaced by CaCl2 to prevent DNA cleavage by BspMI. .. AFM imaging We imaged all samples on a commercial AFM (Cypher ES, Asylum Research) featuring a temperature-controlled (19°C), closed-fluidic sample holder.

    Purification:

    Article Title: Incorporation of bridged nucleic acids into CRISPR RNAs improves Cas9 endonuclease specificity
    Article Snippet: Pre-selection libraries were also separately incubated with 2 U of BspMI (NEB) in NEBuffer 3.1 for 1 h at 37 °C. .. Cas9-digested and BspMI-digested library members were purified with the QiaQuick PCR Purification Kit (Qiagen) and ligated to 10 pmol adaptor1/2(#) (post-selection) or lib adapter 1/lib adapter 2 (pre-selection) (sequences in Supplementary Table ) with 1000 U of T4 DNA Ligase (NEB) in NEB T4 DNA Ligase Reaction Buffer for 16 h at room temperature. .. Adapter ligated DNA was purified using the QiaQuick PCR Purification Kit (Qiagen) and PCR amplified for 19–24 cycles with Q5 Hot Start High-Fidelity DNA Polymerase (NEB) in Q5 Reaction Buffer using primers PE2 short/sel PCR (post-selection) or primers lib seq PCR/lib fwd PCR (pre-selection) (sequences in Supplementary Table ).

    Polymerase Chain Reaction:

    Article Title: Incorporation of bridged nucleic acids into CRISPR RNAs improves Cas9 endonuclease specificity
    Article Snippet: Pre-selection libraries were also separately incubated with 2 U of BspMI (NEB) in NEBuffer 3.1 for 1 h at 37 °C. .. Cas9-digested and BspMI-digested library members were purified with the QiaQuick PCR Purification Kit (Qiagen) and ligated to 10 pmol adaptor1/2(#) (post-selection) or lib adapter 1/lib adapter 2 (pre-selection) (sequences in Supplementary Table ) with 1000 U of T4 DNA Ligase (NEB) in NEB T4 DNA Ligase Reaction Buffer for 16 h at room temperature. .. Adapter ligated DNA was purified using the QiaQuick PCR Purification Kit (Qiagen) and PCR amplified for 19–24 cycles with Q5 Hot Start High-Fidelity DNA Polymerase (NEB) in Q5 Reaction Buffer using primers PE2 short/sel PCR (post-selection) or primers lib seq PCR/lib fwd PCR (pre-selection) (sequences in Supplementary Table ).

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  • 93
    New England Biolabs bspmi enzymes
    Bspmi Enzymes, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bspmi enzymes/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bspmi enzymes - by Bioz Stars, 2021-07
    93/100 stars
      Buy from Supplier

    99
    New England Biolabs bspmi
    Bspmi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bspmi/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bspmi - by Bioz Stars, 2021-07
    99/100 stars
      Buy from Supplier

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