bsp mi  (New England Biolabs)


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    Name:
    BspMI
    Description:
    BspMI 100 units
    Catalog Number:
    r0502l
    Price:
    68
    Size:
    100 units
    Category:
    Restriction Enzymes
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    Structured Review

    New England Biolabs bsp mi
    BspMI
    BspMI 100 units
    https://www.bioz.com/result/bsp mi/product/New England Biolabs
    Average 99 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    bsp mi - by Bioz Stars, 2020-09
    99/100 stars

    Images

    1) Product Images from "Genotyping the GALNT14 gene by joint analysis of two linked single nucleotide polymorphisms using liver tissues for clinical and geographical comparisons"

    Article Title: Genotyping the GALNT14 gene by joint analysis of two linked single nucleotide polymorphisms using liver tissues for clinical and geographical comparisons

    Journal: Oncology Letters

    doi: 10.3892/ol.2014.2507

    Polymerase chain reaction (PCR)-generated double restriction enzyme sites genotyping assay. The central sequence was the reference genomic DNA sequence. This assay was a nested PCR assay where two primer sets, the outer and inner primers, were used. Each of the inner primers had one base mismatch to the reference sequence for creating a restriction site. The cutting site of Bsm AI (GTCTC) was created if the allele type on rs9679162 was G (corresponding to C in the other strand). The cutting site of Bsp MI (ACCTGC) was created if the allele type on rs6752303 was C.
    Figure Legend Snippet: Polymerase chain reaction (PCR)-generated double restriction enzyme sites genotyping assay. The central sequence was the reference genomic DNA sequence. This assay was a nested PCR assay where two primer sets, the outer and inner primers, were used. Each of the inner primers had one base mismatch to the reference sequence for creating a restriction site. The cutting site of Bsm AI (GTCTC) was created if the allele type on rs9679162 was G (corresponding to C in the other strand). The cutting site of Bsp MI (ACCTGC) was created if the allele type on rs6752303 was C.

    Techniques Used: Polymerase Chain Reaction, Generated, Genotyping Assay, Sequencing, Nested PCR

    2) Product Images from "Vesicle-independent extracellular release of a proinflammatory outer membrane lipoprotein in free-soluble form"

    Article Title: Vesicle-independent extracellular release of a proinflammatory outer membrane lipoprotein in free-soluble form

    Journal: BMC Microbiology

    doi: 10.1186/1471-2180-8-18

    PCR-RFLP of pal and detection of its gene product, PAL, from clonally diverse A. actinomycetemcomitans strains. Panel A: Agarose gel electrophoresis of the PCR products shows amplicons with the expected size of pal (425 bp) for each strain. Panels B and C: Agarose gel electrophoresis of the purified PCR amplicons digested with Dde I and Bsp MI, separately. Panel D: immunoblot analysis of the A. actinomycetemcomitans whole cell protein preparations using anti-AaPAL peptide antiserum shows the expected 17-kDa signal for each strain. Lanes 1 through 12 strain identification (serotype; genotype): ATCC 29523 (a; 1), SA5002 (a; 1), ATCC 43718 (b; 2), SA5003 (b; 8), ATCC 33384 (c; 3), SA5005 (c; 3), SA5001 (d; 5), SA5007 (d; 22), SA5008 (e; 6), SA5011 (e; 20), CU1000R (f; nd*), SA5022 (f; 19) and standards (S). *nd: not determined.
    Figure Legend Snippet: PCR-RFLP of pal and detection of its gene product, PAL, from clonally diverse A. actinomycetemcomitans strains. Panel A: Agarose gel electrophoresis of the PCR products shows amplicons with the expected size of pal (425 bp) for each strain. Panels B and C: Agarose gel electrophoresis of the purified PCR amplicons digested with Dde I and Bsp MI, separately. Panel D: immunoblot analysis of the A. actinomycetemcomitans whole cell protein preparations using anti-AaPAL peptide antiserum shows the expected 17-kDa signal for each strain. Lanes 1 through 12 strain identification (serotype; genotype): ATCC 29523 (a; 1), SA5002 (a; 1), ATCC 43718 (b; 2), SA5003 (b; 8), ATCC 33384 (c; 3), SA5005 (c; 3), SA5001 (d; 5), SA5007 (d; 22), SA5008 (e; 6), SA5011 (e; 20), CU1000R (f; nd*), SA5022 (f; 19) and standards (S). *nd: not determined.

    Techniques Used: Polymerase Chain Reaction, Agarose Gel Electrophoresis, Purification

    Related Articles

    Countercurrent Chromatography:

    Article Title: Anti-Candidal Activity and Functional Mapping of Recombinant and Synthetic Neosartorya fischeri Antifungal Protein 2 (NFAP2)
    Article Snippet: .. The paf 5′- and 3′-UTR flanking construct was amplified (Q5 High Fidelity DNA Polymerase, New England Biolabs, Ipswich, MA, USA) from this vector using the primer pair 1stF (5′- GGC CCC ATG GTG GTA AAC AAG TAG TG−3′) / 1stR (5′- GGT GGC GGC CGC TCT AGA ACT AG−3′), and after digestion with BspM I, Not I (New England Biolabs, Ipswich, MA, USA) the resulted 1033 bp amplicon was inserted into the respectively digested pSK275paf vector (Sonderegger et al., ) resulting in the expression vector pSK275nfap2 . ..

    Nucleic Acid Electrophoresis:

    Article Title: Vesicle-independent extracellular release of a proinflammatory outer membrane lipoprotein in free-soluble form
    Article Snippet: .. PCR-restriction fragment length polymorphism (PCR-RFLP) of the A. actinomycetemcomitans pal sequence For RFLP, PCR amplicons generated by the primers PAL-SA 5'-GCTGGTTCTGTGGCTGTGT and PAL-MK 5'-ACTGCACGACGGTTTTTAGC were purified (QIAquick® Gel Extraction Kit, Qiagen GmbH, Helden, Germany) and digested with Bsp MI and Dde I (New England BioLabs, Beverly, MA, USA), respectively, prior to analysis on agarose (1.8%) gel electrophoresis. ..

    Amplification:

    Article Title: Anti-Candidal Activity and Functional Mapping of Recombinant and Synthetic Neosartorya fischeri Antifungal Protein 2 (NFAP2)
    Article Snippet: .. The paf 5′- and 3′-UTR flanking construct was amplified (Q5 High Fidelity DNA Polymerase, New England Biolabs, Ipswich, MA, USA) from this vector using the primer pair 1stF (5′- GGC CCC ATG GTG GTA AAC AAG TAG TG−3′) / 1stR (5′- GGT GGC GGC CGC TCT AGA ACT AG−3′), and after digestion with BspM I, Not I (New England Biolabs, Ipswich, MA, USA) the resulted 1033 bp amplicon was inserted into the respectively digested pSK275paf vector (Sonderegger et al., ) resulting in the expression vector pSK275nfap2 . ..

    Article Title: Genotyping the GALNT14 gene by joint analysis of two linked single nucleotide polymorphisms using liver tissues for clinical and geographical comparisons
    Article Snippet: .. The reaction mixture comprised the second amplicon (3 μl), 10X buffer (2 μl), restriction enzymes Bsm AI or Bsp MI (1 μl) (New England Biolab, Ipswich, MA, USA) and water (14 μl). .. Incubation temperatures were 55°C for Bsm AI and 37°C for Bsp MI.

    Modification:

    Article Title: Bacillus anthracis Virulence Regulator AtxA Binds Specifically to the pagA Promoter Region
    Article Snippet: .. The modified gene was then cut out with SwaI and BspMI enzymes (New England BioLabs) and ligated into appropriately digested pKA2 plasmid. .. The mutated pKA2 plasmid pool was transformed into E. coli One Shot TOP10 competent cells (Invitrogen Corporation), following the manufacturer’s protocol, and then into E. coli SCS110 competent cells (Agilent Technologies).

    Ligation:

    Article Title: Antiviral activity of recombinant ankyrin targeted to the capsid domain of HIV-1 Gag polyprotein
    Article Snippet: .. Once ankyrin repeats were ligated with N-Cap, vector was cleaved with Bsp M I (NEB) and recircularised by intramolecular ligation. .. This resulted in the elimination of the Rep cloning sites regions and its replacement by a variable number of ankyrin repeats between the N- and C-caps.

    Generated:

    Article Title: Vesicle-independent extracellular release of a proinflammatory outer membrane lipoprotein in free-soluble form
    Article Snippet: .. PCR-restriction fragment length polymorphism (PCR-RFLP) of the A. actinomycetemcomitans pal sequence For RFLP, PCR amplicons generated by the primers PAL-SA 5'-GCTGGTTCTGTGGCTGTGT and PAL-MK 5'-ACTGCACGACGGTTTTTAGC were purified (QIAquick® Gel Extraction Kit, Qiagen GmbH, Helden, Germany) and digested with Bsp MI and Dde I (New England BioLabs, Beverly, MA, USA), respectively, prior to analysis on agarose (1.8%) gel electrophoresis. ..

    Construct:

    Article Title: Anti-Candidal Activity and Functional Mapping of Recombinant and Synthetic Neosartorya fischeri Antifungal Protein 2 (NFAP2)
    Article Snippet: .. The paf 5′- and 3′-UTR flanking construct was amplified (Q5 High Fidelity DNA Polymerase, New England Biolabs, Ipswich, MA, USA) from this vector using the primer pair 1stF (5′- GGC CCC ATG GTG GTA AAC AAG TAG TG−3′) / 1stR (5′- GGT GGC GGC CGC TCT AGA ACT AG−3′), and after digestion with BspM I, Not I (New England Biolabs, Ipswich, MA, USA) the resulted 1033 bp amplicon was inserted into the respectively digested pSK275paf vector (Sonderegger et al., ) resulting in the expression vector pSK275nfap2 . ..

    Purification:

    Article Title: Vesicle-independent extracellular release of a proinflammatory outer membrane lipoprotein in free-soluble form
    Article Snippet: .. PCR-restriction fragment length polymorphism (PCR-RFLP) of the A. actinomycetemcomitans pal sequence For RFLP, PCR amplicons generated by the primers PAL-SA 5'-GCTGGTTCTGTGGCTGTGT and PAL-MK 5'-ACTGCACGACGGTTTTTAGC were purified (QIAquick® Gel Extraction Kit, Qiagen GmbH, Helden, Germany) and digested with Bsp MI and Dde I (New England BioLabs, Beverly, MA, USA), respectively, prior to analysis on agarose (1.8%) gel electrophoresis. ..

    Article Title: Incorporation of bridged nucleic acids into CRISPR RNAs improves Cas9 endonuclease specificity
    Article Snippet: .. Cas9-digested and BspMI-digested library members were purified with the QiaQuick PCR Purification Kit (Qiagen) and ligated to 10 pmol adaptor1/2(#) (post-selection) or lib adapter 1/lib adapter 2 (pre-selection) (sequences in Supplementary Table ) with 1000 U of T4 DNA Ligase (NEB) in NEB T4 DNA Ligase Reaction Buffer for 16 h at room temperature. .. Adapter ligated DNA was purified using the QiaQuick PCR Purification Kit (Qiagen) and PCR amplified for 19–24 cycles with Q5 Hot Start High-Fidelity DNA Polymerase (NEB) in Q5 Reaction Buffer using primers PE2 short/sel PCR (post-selection) or primers lib seq PCR/lib fwd PCR (pre-selection) (sequences in Supplementary Table ).

    Polymerase Chain Reaction:

    Article Title: Vesicle-independent extracellular release of a proinflammatory outer membrane lipoprotein in free-soluble form
    Article Snippet: .. PCR-restriction fragment length polymorphism (PCR-RFLP) of the A. actinomycetemcomitans pal sequence For RFLP, PCR amplicons generated by the primers PAL-SA 5'-GCTGGTTCTGTGGCTGTGT and PAL-MK 5'-ACTGCACGACGGTTTTTAGC were purified (QIAquick® Gel Extraction Kit, Qiagen GmbH, Helden, Germany) and digested with Bsp MI and Dde I (New England BioLabs, Beverly, MA, USA), respectively, prior to analysis on agarose (1.8%) gel electrophoresis. ..

    Article Title: Incorporation of bridged nucleic acids into CRISPR RNAs improves Cas9 endonuclease specificity
    Article Snippet: .. Cas9-digested and BspMI-digested library members were purified with the QiaQuick PCR Purification Kit (Qiagen) and ligated to 10 pmol adaptor1/2(#) (post-selection) or lib adapter 1/lib adapter 2 (pre-selection) (sequences in Supplementary Table ) with 1000 U of T4 DNA Ligase (NEB) in NEB T4 DNA Ligase Reaction Buffer for 16 h at room temperature. .. Adapter ligated DNA was purified using the QiaQuick PCR Purification Kit (Qiagen) and PCR amplified for 19–24 cycles with Q5 Hot Start High-Fidelity DNA Polymerase (NEB) in Q5 Reaction Buffer using primers PE2 short/sel PCR (post-selection) or primers lib seq PCR/lib fwd PCR (pre-selection) (sequences in Supplementary Table ).

    Incubation:

    Article Title: Bending and looping of long DNA by Polycomb repressive complex 2 revealed by AFM imaging in liquid
    Article Snippet: .. Experiments then proceeded as described elsewhere , except incubation occurred with 100 U/ml of BspMI (New England Biolabs, R0502S) in the buffer, instead of PRC2; MgCl2 was replaced by CaCl2 to prevent DNA cleavage by BspMI. .. AFM imaging We imaged all samples on a commercial AFM (Cypher ES, Asylum Research) featuring a temperature-controlled (19°C), closed-fluidic sample holder.

    Expressing:

    Article Title: Anti-Candidal Activity and Functional Mapping of Recombinant and Synthetic Neosartorya fischeri Antifungal Protein 2 (NFAP2)
    Article Snippet: .. The paf 5′- and 3′-UTR flanking construct was amplified (Q5 High Fidelity DNA Polymerase, New England Biolabs, Ipswich, MA, USA) from this vector using the primer pair 1stF (5′- GGC CCC ATG GTG GTA AAC AAG TAG TG−3′) / 1stR (5′- GGT GGC GGC CGC TCT AGA ACT AG−3′), and after digestion with BspM I, Not I (New England Biolabs, Ipswich, MA, USA) the resulted 1033 bp amplicon was inserted into the respectively digested pSK275paf vector (Sonderegger et al., ) resulting in the expression vector pSK275nfap2 . ..

    Sequencing:

    Article Title: Vesicle-independent extracellular release of a proinflammatory outer membrane lipoprotein in free-soluble form
    Article Snippet: .. PCR-restriction fragment length polymorphism (PCR-RFLP) of the A. actinomycetemcomitans pal sequence For RFLP, PCR amplicons generated by the primers PAL-SA 5'-GCTGGTTCTGTGGCTGTGT and PAL-MK 5'-ACTGCACGACGGTTTTTAGC were purified (QIAquick® Gel Extraction Kit, Qiagen GmbH, Helden, Germany) and digested with Bsp MI and Dde I (New England BioLabs, Beverly, MA, USA), respectively, prior to analysis on agarose (1.8%) gel electrophoresis. ..

    Gel Extraction:

    Article Title: Vesicle-independent extracellular release of a proinflammatory outer membrane lipoprotein in free-soluble form
    Article Snippet: .. PCR-restriction fragment length polymorphism (PCR-RFLP) of the A. actinomycetemcomitans pal sequence For RFLP, PCR amplicons generated by the primers PAL-SA 5'-GCTGGTTCTGTGGCTGTGT and PAL-MK 5'-ACTGCACGACGGTTTTTAGC were purified (QIAquick® Gel Extraction Kit, Qiagen GmbH, Helden, Germany) and digested with Bsp MI and Dde I (New England BioLabs, Beverly, MA, USA), respectively, prior to analysis on agarose (1.8%) gel electrophoresis. ..

    Activated Clotting Time Assay:

    Article Title: Anti-Candidal Activity and Functional Mapping of Recombinant and Synthetic Neosartorya fischeri Antifungal Protein 2 (NFAP2)
    Article Snippet: .. The paf 5′- and 3′-UTR flanking construct was amplified (Q5 High Fidelity DNA Polymerase, New England Biolabs, Ipswich, MA, USA) from this vector using the primer pair 1stF (5′- GGC CCC ATG GTG GTA AAC AAG TAG TG−3′) / 1stR (5′- GGT GGC GGC CGC TCT AGA ACT AG−3′), and after digestion with BspM I, Not I (New England Biolabs, Ipswich, MA, USA) the resulted 1033 bp amplicon was inserted into the respectively digested pSK275paf vector (Sonderegger et al., ) resulting in the expression vector pSK275nfap2 . ..

    Plasmid Preparation:

    Article Title: Bacillus anthracis Virulence Regulator AtxA Binds Specifically to the pagA Promoter Region
    Article Snippet: .. The modified gene was then cut out with SwaI and BspMI enzymes (New England BioLabs) and ligated into appropriately digested pKA2 plasmid. .. The mutated pKA2 plasmid pool was transformed into E. coli One Shot TOP10 competent cells (Invitrogen Corporation), following the manufacturer’s protocol, and then into E. coli SCS110 competent cells (Agilent Technologies).

    Article Title: Anti-Candidal Activity and Functional Mapping of Recombinant and Synthetic Neosartorya fischeri Antifungal Protein 2 (NFAP2)
    Article Snippet: .. The paf 5′- and 3′-UTR flanking construct was amplified (Q5 High Fidelity DNA Polymerase, New England Biolabs, Ipswich, MA, USA) from this vector using the primer pair 1stF (5′- GGC CCC ATG GTG GTA AAC AAG TAG TG−3′) / 1stR (5′- GGT GGC GGC CGC TCT AGA ACT AG−3′), and after digestion with BspM I, Not I (New England Biolabs, Ipswich, MA, USA) the resulted 1033 bp amplicon was inserted into the respectively digested pSK275paf vector (Sonderegger et al., ) resulting in the expression vector pSK275nfap2 . ..

    Article Title: Antiviral activity of recombinant ankyrin targeted to the capsid domain of HIV-1 Gag polyprotein
    Article Snippet: .. Once ankyrin repeats were ligated with N-Cap, vector was cleaved with Bsp M I (NEB) and recircularised by intramolecular ligation. .. This resulted in the elimination of the Rep cloning sites regions and its replacement by a variable number of ankyrin repeats between the N- and C-caps.

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    New England Biolabs bsp mi
    Polymerase chain reaction (PCR)-generated double restriction enzyme sites genotyping assay. The central sequence was the reference genomic DNA sequence. This assay was a nested PCR assay where two primer sets, the outer and inner primers, were used. Each of the inner primers had one base mismatch to the reference sequence for creating a restriction site. The cutting site of <t>Bsm</t> AI (GTCTC) was created if the allele type on rs9679162 was G (corresponding to C in the other strand). The cutting site of <t>Bsp</t> MI (ACCTGC) was created if the allele type on rs6752303 was C.
    Bsp Mi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bsp mi/product/New England Biolabs
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    bsp mi - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

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    Polymerase chain reaction (PCR)-generated double restriction enzyme sites genotyping assay. The central sequence was the reference genomic DNA sequence. This assay was a nested PCR assay where two primer sets, the outer and inner primers, were used. Each of the inner primers had one base mismatch to the reference sequence for creating a restriction site. The cutting site of Bsm AI (GTCTC) was created if the allele type on rs9679162 was G (corresponding to C in the other strand). The cutting site of Bsp MI (ACCTGC) was created if the allele type on rs6752303 was C.

    Journal: Oncology Letters

    Article Title: Genotyping the GALNT14 gene by joint analysis of two linked single nucleotide polymorphisms using liver tissues for clinical and geographical comparisons

    doi: 10.3892/ol.2014.2507

    Figure Lengend Snippet: Polymerase chain reaction (PCR)-generated double restriction enzyme sites genotyping assay. The central sequence was the reference genomic DNA sequence. This assay was a nested PCR assay where two primer sets, the outer and inner primers, were used. Each of the inner primers had one base mismatch to the reference sequence for creating a restriction site. The cutting site of Bsm AI (GTCTC) was created if the allele type on rs9679162 was G (corresponding to C in the other strand). The cutting site of Bsp MI (ACCTGC) was created if the allele type on rs6752303 was C.

    Article Snippet: The reaction mixture comprised the second amplicon (3 μl), 10X buffer (2 μl), restriction enzymes Bsm AI or Bsp MI (1 μl) (New England Biolab, Ipswich, MA, USA) and water (14 μl).

    Techniques: Polymerase Chain Reaction, Generated, Genotyping Assay, Sequencing, Nested PCR

    PCR-RFLP of pal and detection of its gene product, PAL, from clonally diverse A. actinomycetemcomitans strains. Panel A: Agarose gel electrophoresis of the PCR products shows amplicons with the expected size of pal (425 bp) for each strain. Panels B and C: Agarose gel electrophoresis of the purified PCR amplicons digested with Dde I and Bsp MI, separately. Panel D: immunoblot analysis of the A. actinomycetemcomitans whole cell protein preparations using anti-AaPAL peptide antiserum shows the expected 17-kDa signal for each strain. Lanes 1 through 12 strain identification (serotype; genotype): ATCC 29523 (a; 1), SA5002 (a; 1), ATCC 43718 (b; 2), SA5003 (b; 8), ATCC 33384 (c; 3), SA5005 (c; 3), SA5001 (d; 5), SA5007 (d; 22), SA5008 (e; 6), SA5011 (e; 20), CU1000R (f; nd*), SA5022 (f; 19) and standards (S). *nd: not determined.

    Journal: BMC Microbiology

    Article Title: Vesicle-independent extracellular release of a proinflammatory outer membrane lipoprotein in free-soluble form

    doi: 10.1186/1471-2180-8-18

    Figure Lengend Snippet: PCR-RFLP of pal and detection of its gene product, PAL, from clonally diverse A. actinomycetemcomitans strains. Panel A: Agarose gel electrophoresis of the PCR products shows amplicons with the expected size of pal (425 bp) for each strain. Panels B and C: Agarose gel electrophoresis of the purified PCR amplicons digested with Dde I and Bsp MI, separately. Panel D: immunoblot analysis of the A. actinomycetemcomitans whole cell protein preparations using anti-AaPAL peptide antiserum shows the expected 17-kDa signal for each strain. Lanes 1 through 12 strain identification (serotype; genotype): ATCC 29523 (a; 1), SA5002 (a; 1), ATCC 43718 (b; 2), SA5003 (b; 8), ATCC 33384 (c; 3), SA5005 (c; 3), SA5001 (d; 5), SA5007 (d; 22), SA5008 (e; 6), SA5011 (e; 20), CU1000R (f; nd*), SA5022 (f; 19) and standards (S). *nd: not determined.

    Article Snippet: PCR-restriction fragment length polymorphism (PCR-RFLP) of the A. actinomycetemcomitans pal sequence For RFLP, PCR amplicons generated by the primers PAL-SA 5'-GCTGGTTCTGTGGCTGTGT and PAL-MK 5'-ACTGCACGACGGTTTTTAGC were purified (QIAquick® Gel Extraction Kit, Qiagen GmbH, Helden, Germany) and digested with Bsp MI and Dde I (New England BioLabs, Beverly, MA, USA), respectively, prior to analysis on agarose (1.8%) gel electrophoresis.

    Techniques: Polymerase Chain Reaction, Agarose Gel Electrophoresis, Purification