styi restriction endonuclease  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90

    Structured Review

    New England Biolabs styi restriction endonuclease
    Styi Restriction Endonuclease, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/styi restriction endonuclease/product/New England Biolabs
    Average 90 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    styi restriction endonuclease - by Bioz Stars, 2020-03
    90/100 stars

    Related Products / Commonly Used Together

    pcr reaction
    xhoi
    1x ligation buffer
    rflp analysis
    amplicon

    Images

    Related Articles

    Diagnostic Assay:

    Article Title: Molecular and Phenotypic Analyses Reveal Association of Diverse Colletotrichum acutatum Groups and a Low Level of C. gloeosporioides with Olive Anthracnose
    Article Snippet: Similarly, primers CaInt2 specific for C. acutatum ( ) and CgInt specific for C. gloeosporioides ( ) were each used in conjunction with the conserved primer ITS4 ( ) for diagnostic PCR based on the rRNA gene-ITS region. .. For the diagnosis of molecular groups within C. acutatum based on unique restriction fragment profiles, the tub2 fragment amplified with primers TB5 and TBCA (specific for C. acutatum ) and TB5 and TB6 (conserved) ( ) were restriction digested using enzymes StyI, NlaIII, RsaI (New England Biolabs, Hitchin, United Kingdom), and SacI (Roche Diagnostics, Mannheim, Germany).

    Clone Assay:

    Article Title: Glycine-alanine dipeptide repeat protein contributes to toxicity in a zebrafish model of C9orf72 associated neurodegeneration
    Article Snippet: .. The pCS2 + ATG-80xGGGGCC-GFP and pCS2 + 80xGGGGCC plasmids were digested by StyI (New England Biolabs) and HpaI (New England Biolabs) and cloned into pT2KXIGdeltaIN plasmid (a gift from K. Kawakami, National Institute of Genetics, Shizuoka, Japan) to generate transgenic zebrafish by TOL2 mediated transposition. .. Our pT2KXIGdeltaIN plasmid harbored one point mutation in the UAS region that generated a novel StyI recognition site.

    Centrifugation:

    Article Title: Structural Features of Nucleosomes Reorganized by Yeast FACT and Its HMG Box Component, Nhp6
    Article Snippet: For denaturing polyacrylamide gel electrophoresis (PAGE) analysis, samples were extracted with 150 μl of CHCl3 containing 1/25 volume of isoamyl alcohol, the aqueous phase was precipitated with 2.5 volumes of 95% ethanol, and the DNA was recovered by centrifugation. .. DraI, HhaI, and StyI (New England Biolabs) were used in the same way except the NaCl was omitted from the binding reaction and replaced with 1× reaction buffer supplied with the enzyme, and 20 U of each enzyme was added per 100 fmol of DNA.

    Amplification:

    Article Title: Genome-wide molecular characterization of central nervous system primitive neuroectodermal tumor and pineoblastoma
    Article Snippet: DNA digestion, labeling, and hybridization were performed on the basis of the Affymetrix 100K or 500K SNP array protocols., In brief, 250 ng of DNA was digested (with either Xba1 or HindIII [New England Biolabs] for each 50K SNP array and NspI or StyI [New England Biolabs] for each 250K SNP array). .. After adaptor ligation, samples were amplified by polymerase chain reaction (PCR) using Titanium Taq DNA polymerase (Clontech) for the 100K assay or AmpliTag Gold (Applied Biosystems) for the 500K assay.

    Article Title: SNPs Array Karyotyping Reveals a Novel Recurrent 20p13 Amplification in Primary Myelofibrosis
    Article Snippet: First DNA was cut by restriction enzymes NspI and StyI (Nsp I Enzyme, Sty I Enzyme by New England Biolabs) followed by adaptor ligation (T4 DNA Ligase by New England Biolabs). .. A generic primer that recognizes the adaptor sequence was used to amplify adaptor-ligated DNA fragments (TITANIUM DNA Amplification Kit by Clontech).

    Article Title: North Carolina macular dystrophy (MCDR1) caused by a novel tandem duplication of the PRDM13 gene
    Article Snippet: .. Briefly, DNA was digested with NspI and StyI restriction enzymes (New England Biolabs, Ipswich, MA), ligated to adapters and amplified using adapter-specific primers. .. PCR products were fragmented, labeled and loaded on the SNP 6.0 arrays.

    Article Title: Tailoring RNA modular units on a common scaffold: A modular ribozyme with a catalytic unit for ?-nicotinamide mononucleotide-activated RNA ligation
    Article Snippet: Three pool-L oligonucleotides (pool L-1, pool L-2, and pool L-3) including 15, 10, and 5 nt of random sequence, respectively, were amplified by PCR using a sense primer (Fw-L) and an antisense primer (Rv-1). .. Half of each PCR product was digested with BanI (New England Biolabs), and the remaining half was digested with StyI (New England Biolabs).

    Article Title: Glycine-alanine dipeptide repeat protein contributes to toxicity in a zebrafish model of C9orf72 associated neurodegeneration
    Article Snippet: The fragment was then ligated to a PCR amplified pCS2+ plasmid vector backbone. .. The pCS2 + ATG-80xGGGGCC-GFP and pCS2 + 80xGGGGCC plasmids were digested by StyI (New England Biolabs) and HpaI (New England Biolabs) and cloned into pT2KXIGdeltaIN plasmid (a gift from K. Kawakami, National Institute of Genetics, Shizuoka, Japan) to generate transgenic zebrafish by TOL2 mediated transposition.

    Article Title: Molecular and Phenotypic Analyses Reveal Association of Diverse Colletotrichum acutatum Groups and a Low Level of C. gloeosporioides with Olive Anthracnose
    Article Snippet: .. For the diagnosis of molecular groups within C. acutatum based on unique restriction fragment profiles, the tub2 fragment amplified with primers TB5 and TBCA (specific for C. acutatum ) and TB5 and TB6 (conserved) ( ) were restriction digested using enzymes StyI, NlaIII, RsaI (New England Biolabs, Hitchin, United Kingdom), and SacI (Roche Diagnostics, Mannheim, Germany). ..

    Article Title: A Long Noncoding RNA Controls Muscle Differentiation by Functioning as a Competing Endogenous RNA
    Article Snippet: DNA was digested with 800 units of StyI restriction enzyme and ligated in 1X ligation buffer (NEB). .. First, to ensure that primer efficiency does not introduce bias, a control template was generated by digesting and ligating equimolar amounts of all possible PCR products and used to calculate amplification efficiency of each primer pairs.

    Article Title: Quality Assurance for Duchenne and Becker Muscular Dystrophy Genetic Testing
    Article Snippet: In brief, 250 ng of genomic DNA was digested with either Nsp 1 or StyI (New England Biolabs, Inc., Ipswich, MA). .. PCR master mix (Titanium DNA Amplification Kit; Clontech Laboratories Inc., Mountain View, CA) was added to each well, and the reactions were cycled as follows: 94°C for 3 minutes; 30 cycles of 94°C for 30 seconds, 60°C for 45 seconds, and 68°C for 15 seconds; 68°C for 7 minutes; and 4°C hold.

    Article Title: Deletion of ETS-1, a gene in the Jacobsen syndrome critical region, causes ventricular septal defects and abnormal ventricular morphology in mice
    Article Snippet: In short, 250 ng of genomic DNA is digested with 10 units of NspI or StyI (New England Biolabs, Beverly, MA, USA) for 2 h at 37°C. .. After dilution with water, samples were subjected to PCR using primers specific to the adaptor sequence with the following amplification parameters: 95°C for 3 min (initial denaturation), 95°C for 20 s, 59°C for 15 s, 72°C for 15 s for a total of 35 cycles, followed by 72°C for 7 min (final extension).

    Article Title: Molecular typing reveals substantial Plasmodium vivax infection in asymptomatic adults in a rural area of Cameroon
    Article Snippet: .. Duffy blood group determination To detect the point mutation -33 T → C, which corresponds to a Duffy-negative phenotype, the DARC gene promoter region of 13 subjects were amplified directly from frozen packed red and white cells by PCR, followed by wet lab enzymatic restriction digestion with StyI (New England Biolabs, Ipswich, MA), as well as in silico StyI enzymatic restriction digestion. .. The PCR was done with primer pairs previously published [ ] with a slight modification of the PCR constituents.

    Polymerase Chain Reaction:

    Article Title: Genome-wide molecular characterization of central nervous system primitive neuroectodermal tumor and pineoblastoma
    Article Snippet: DNA digestion, labeling, and hybridization were performed on the basis of the Affymetrix 100K or 500K SNP array protocols., In brief, 250 ng of DNA was digested (with either Xba1 or HindIII [New England Biolabs] for each 50K SNP array and NspI or StyI [New England Biolabs] for each 250K SNP array). .. After adaptor ligation, samples were amplified by polymerase chain reaction (PCR) using Titanium Taq DNA polymerase (Clontech) for the 100K assay or AmpliTag Gold (Applied Biosystems) for the 500K assay.

    Article Title: By Inhibiting Replication, the Large Hepatitis Delta Antigen Can Indirectly Regulate Amber/W Editing and Its Own Expression
    Article Snippet: .. Ten microliters of the second PCR samples was digested to completion with 20 U of StyI (New England Biolabs) in 1× NEB buffer 3 in a total volume of 20 μl. .. The digest was analyzed by electrophoresis on a 1.5% agarose gel, and the gel was stained with ethidium bromide.

    Article Title: The Novel Mouse Mutation Oblivion Inactivates the PMCA2 Pump and Causes Progressive Hearing Loss
    Article Snippet: .. The 111 bp PCR product was digested with StyI (New England Biolabs) which cuts the wildtype allele giving an 89 bp fragment while the mutant allele remains uncut. .. This genotyping tool was used to establish whether the sequence change in Obl DNA was a polymorphism in a total of 17 inbred strains: BALB/C, CBA/Ca, C3HeB/FeJ, DDY/Jc1, 129X1/SvJ, A/J, Bxd-1/Ty, C58/J, CE/J, DA/HuSn, DBA/2J, FL/1Re, LP/J, NON/LtJ, RBG/Dn, St/bJ and SWR/J.

    Article Title: North Carolina macular dystrophy (MCDR1) caused by a novel tandem duplication of the PRDM13 gene
    Article Snippet: Briefly, DNA was digested with NspI and StyI restriction enzymes (New England Biolabs, Ipswich, MA), ligated to adapters and amplified using adapter-specific primers. .. PCR products were fragmented, labeled and loaded on the SNP 6.0 arrays.

    Article Title: Tailoring RNA modular units on a common scaffold: A modular ribozyme with a catalytic unit for ?-nicotinamide mononucleotide-activated RNA ligation
    Article Snippet: .. Half of each PCR product was digested with BanI (New England Biolabs), and the remaining half was digested with StyI (New England Biolabs). .. The R-1, R-2, and R-3 PCR fragments were ligated with L-1, L-2, and L-3 fragments, respectively, using T4 DNA ligase (Takara).

    Article Title: Glycine-alanine dipeptide repeat protein contributes to toxicity in a zebrafish model of C9orf72 associated neurodegeneration
    Article Snippet: The fragment was then ligated to a PCR amplified pCS2+ plasmid vector backbone. .. The pCS2 + ATG-80xGGGGCC-GFP and pCS2 + 80xGGGGCC plasmids were digested by StyI (New England Biolabs) and HpaI (New England Biolabs) and cloned into pT2KXIGdeltaIN plasmid (a gift from K. Kawakami, National Institute of Genetics, Shizuoka, Japan) to generate transgenic zebrafish by TOL2 mediated transposition.

    Article Title: Molecular and Phenotypic Analyses Reveal Association of Diverse Colletotrichum acutatum Groups and a Low Level of C. gloeosporioides with Olive Anthracnose
    Article Snippet: Paragraph title: PCR based detection of pathogens and diagnosis of molecular groups within C. acutatum . ... For the diagnosis of molecular groups within C. acutatum based on unique restriction fragment profiles, the tub2 fragment amplified with primers TB5 and TBCA (specific for C. acutatum ) and TB5 and TB6 (conserved) ( ) were restriction digested using enzymes StyI, NlaIII, RsaI (New England Biolabs, Hitchin, United Kingdom), and SacI (Roche Diagnostics, Mannheim, Germany).

    Article Title: A Long Noncoding RNA Controls Muscle Differentiation by Functioning as a Competing Endogenous RNA
    Article Snippet: DNA was digested with 800 units of StyI restriction enzyme and ligated in 1X ligation buffer (NEB). .. Ligation products were purified using QIAquick PCR purification kit (QIAGEN).

    Article Title: Quality Assurance for Duchenne and Becker Muscular Dystrophy Genetic Testing
    Article Snippet: In brief, 250 ng of genomic DNA was digested with either Nsp 1 or StyI (New England Biolabs, Inc., Ipswich, MA). .. PCR master mix (Titanium DNA Amplification Kit; Clontech Laboratories Inc., Mountain View, CA) was added to each well, and the reactions were cycled as follows: 94°C for 3 minutes; 30 cycles of 94°C for 30 seconds, 60°C for 45 seconds, and 68°C for 15 seconds; 68°C for 7 minutes; and 4°C hold.

    Article Title: Deletion of ETS-1, a gene in the Jacobsen syndrome critical region, causes ventricular septal defects and abnormal ventricular morphology in mice
    Article Snippet: In short, 250 ng of genomic DNA is digested with 10 units of NspI or StyI (New England Biolabs, Beverly, MA, USA) for 2 h at 37°C. .. After dilution with water, samples were subjected to PCR using primers specific to the adaptor sequence with the following amplification parameters: 95°C for 3 min (initial denaturation), 95°C for 20 s, 59°C for 15 s, 72°C for 15 s for a total of 35 cycles, followed by 72°C for 7 min (final extension).

    Article Title: Molecular typing reveals substantial Plasmodium vivax infection in asymptomatic adults in a rural area of Cameroon
    Article Snippet: .. Duffy blood group determination To detect the point mutation -33 T → C, which corresponds to a Duffy-negative phenotype, the DARC gene promoter region of 13 subjects were amplified directly from frozen packed red and white cells by PCR, followed by wet lab enzymatic restriction digestion with StyI (New England Biolabs, Ipswich, MA), as well as in silico StyI enzymatic restriction digestion. .. The PCR was done with primer pairs previously published [ ] with a slight modification of the PCR constituents.

    Autoradiography:

    Article Title: Structural Features of Nucleosomes Reorganized by Yeast FACT and Its HMG Box Component, Nhp6
    Article Snippet: DraI, HhaI, and StyI (New England Biolabs) were used in the same way except the NaCl was omitted from the binding reaction and replaced with 1× reaction buffer supplied with the enzyme, and 20 U of each enzyme was added per 100 fmol of DNA. .. Nucleosomes were detected by autoradiography, regions of interest were excised, and the DNA was recovered from the gel fragments by overnight extraction at 37°C with 0.2% sodium dodecyl sulfate in 20 mM Tris-HCl (pH 7.5), followed by precipitation with ethanol and PAGE as described above.

    Article Title: A multi-systemic mitochondrial disorder due to a dominant p.Y955H disease variant in DNA polymerase gamma
    Article Snippet: Approximately 1–3 µg of total DNA was cut using either XhoI or StyI restriction endonuclease (NEB). .. COXI or ND6-CytB (positions 10169–11169) were used as probes, and signals were visualized by autoradiography.

    Construct:

    Article Title: Tailoring RNA modular units on a common scaffold: A modular ribozyme with a catalytic unit for ?-nicotinamide mononucleotide-activated RNA ligation
    Article Snippet: Template DNAs for three sublibraries were constructed as follows. .. Half of each PCR product was digested with BanI (New England Biolabs), and the remaining half was digested with StyI (New England Biolabs).

    Article Title: Glycine-alanine dipeptide repeat protein contributes to toxicity in a zebrafish model of C9orf72 associated neurodegeneration
    Article Snippet: For the generation of the GA80-GFP and ggggcc80-GFP plasmids were constructed as described in Additional file : Figure S7. pCS2 + eGFP plasmid [ ] was PCR amplified by Phusion high fidelity polymerase (New England Biolabs) using the following primers: GA80-GFP A: pCS2-f1: 5′-ggccgcaGGTGGCGGAGGTGGCGTGAGCAAGGGCGAGGAGC-3′ pCS2-r1: 5′-gCATGGTGGCGGCCTTGGATCCGGAATTCGAATCGATGGGATCCTGCA-3′ B: pCS2-f2: 5′- gcaGGTGGCGGAGGTGGCGTGAGCAAGGGCGAGGAGC-3′ pCS2-r2: 5′- tagCATGGTGGCGGCCTTGGATCCGGAATTCGAATCGATGGGATCCTGCA-3′ ggggcc80xRNA A’: pCS2-f1: 5′- ggccgcaGGTGGCGGAGGTGGCGTGAGCAAGGGCGAGGAGC-3′ pCS2-r3: 5′- gGGTGGCGGCCTTGGATCCGGAATTCGAATCGATGGGATCCTGCA-3′ B’ pCS2-f2: 5′- gcaGGTGGCGGAGGTGGCGTGAGCAAGGGCGAGGAGC-3′ pCS2-r2: 5′- tagCATGGTGGCGGCCTTGGATCCGGAATTCGAATCGATGGGATCCTGCA-3′ After purification of the PCR products generated by A/B or A’/B’, the PCR products were co-incubated at following cycles (94 °C 2 min, 94 °C 30 s, 55 °C 30 s, 72 °C 2 min, 72 °C 10 min, 10 °C 10 min) to produce sticky end fragments digested by NotI/BfaI (New England Biolabs), like for circular plasmid generation. .. The pCS2 + ATG-80xGGGGCC-GFP and pCS2 + 80xGGGGCC plasmids were digested by StyI (New England Biolabs) and HpaI (New England Biolabs) and cloned into pT2KXIGdeltaIN plasmid (a gift from K. Kawakami, National Institute of Genetics, Shizuoka, Japan) to generate transgenic zebrafish by TOL2 mediated transposition.

    Electrophoresis:

    Article Title: Structural Features of Nucleosomes Reorganized by Yeast FACT and Its HMG Box Component, Nhp6
    Article Snippet: Samples were incubated for several minutes at 65°C and then subjected to electrophoresis through 0.4-mm gels consisting of 8% polyacrylamide (0.4% bisacrylamide), 7 M urea, and 70% Tris-borate-EDTA ( ). .. DraI, HhaI, and StyI (New England Biolabs) were used in the same way except the NaCl was omitted from the binding reaction and replaced with 1× reaction buffer supplied with the enzyme, and 20 U of each enzyme was added per 100 fmol of DNA.

    Article Title: By Inhibiting Replication, the Large Hepatitis Delta Antigen Can Indirectly Regulate Amber/W Editing and Its Own Expression
    Article Snippet: Ten microliters of the second PCR samples was digested to completion with 20 U of StyI (New England Biolabs) in 1× NEB buffer 3 in a total volume of 20 μl. .. The digest was analyzed by electrophoresis on a 1.5% agarose gel, and the gel was stained with ethidium bromide.

    Article Title: Molecular and Phenotypic Analyses Reveal Association of Diverse Colletotrichum acutatum Groups and a Low Level of C. gloeosporioides with Olive Anthracnose
    Article Snippet: PCR products (each, 10 μl) were visualized by electrophoresis on 2% (wt/vol) agarose gels (Invitrogen, Paisley, United Kingdom). .. For the diagnosis of molecular groups within C. acutatum based on unique restriction fragment profiles, the tub2 fragment amplified with primers TB5 and TBCA (specific for C. acutatum ) and TB5 and TB6 (conserved) ( ) were restriction digested using enzymes StyI, NlaIII, RsaI (New England Biolabs, Hitchin, United Kingdom), and SacI (Roche Diagnostics, Mannheim, Germany).

    Microarray:

    Article Title: Deletion of ETS-1, a gene in the Jacobsen syndrome critical region, causes ventricular septal defects and abnormal ventricular morphology in mice
    Article Snippet: Paragraph title: Chromosomal microarray mapping (CMM) ... In short, 250 ng of genomic DNA is digested with 10 units of NspI or StyI (New England Biolabs, Beverly, MA, USA) for 2 h at 37°C.

    Incubation:

    Article Title: Structural Features of Nucleosomes Reorganized by Yeast FACT and Its HMG Box Component, Nhp6
    Article Snippet: Exonuclease III (New England Biolabs) digestions were performed as for DNase I except that various amounts of exonuclease III were added and incubated at 30°C for 10 min before the reaction was terminated and samples were prepared for denaturing PAGE as above. .. DraI, HhaI, and StyI (New England Biolabs) were used in the same way except the NaCl was omitted from the binding reaction and replaced with 1× reaction buffer supplied with the enzyme, and 20 U of each enzyme was added per 100 fmol of DNA.

    Article Title: North Carolina macular dystrophy (MCDR1) caused by a novel tandem duplication of the PRDM13 gene
    Article Snippet: The collection tubes containing saliva were incubated at 50° C and DNA extracted using the manufacture’s protocol with slight modifications as described previously [ ]. .. Briefly, DNA was digested with NspI and StyI restriction enzymes (New England Biolabs, Ipswich, MA), ligated to adapters and amplified using adapter-specific primers.

    Article Title: A multi-systemic mitochondrial disorder due to a dominant p.Y955H disease variant in DNA polymerase gamma
    Article Snippet: After incubation at 65 °C for 30 min, 800 µl of freshly prepared Buffer B (4 µl of 5 M KOAc and 10 µl of 6 M lithium chloride) was added and samples were left on ice for 120 min. After incubation, samples were centrifuged at 12 000g for 15 min and supernatant was transferred into a new tube. .. Approximately 1–3 µg of total DNA was cut using either XhoI or StyI restriction endonuclease (NEB).

    Introduce:

    Article Title: A Long Noncoding RNA Controls Muscle Differentiation by Functioning as a Competing Endogenous RNA
    Article Snippet: DNA was digested with 800 units of StyI restriction enzyme and ligated in 1X ligation buffer (NEB). .. First, to ensure that primer efficiency does not introduce bias, a control template was generated by digesting and ligating equimolar amounts of all possible PCR products and used to calculate amplification efficiency of each primer pairs.

    Genome Wide:

    Article Title: Genome-wide molecular characterization of central nervous system primitive neuroectodermal tumor and pineoblastoma
    Article Snippet: Twenty-four CNS PNETs and 8 pineoblastomas were analyzed using the genechip human mapping 100K set (Affymetrix) to identify genome-wide copy number alterations. .. DNA digestion, labeling, and hybridization were performed on the basis of the Affymetrix 100K or 500K SNP array protocols., In brief, 250 ng of DNA was digested (with either Xba1 or HindIII [New England Biolabs] for each 50K SNP array and NspI or StyI [New England Biolabs] for each 250K SNP array).

    Article Title: SNPs Array Karyotyping Reveals a Novel Recurrent 20p13 Amplification in Primary Myelofibrosis
    Article Snippet: Briefly, 500 ng of highly purified genomic DNA from each patient was processed in each step by a provided Affymetrix® Genome-Wide Human SNP Nsp/Sty Assay Kit 5.0/6. and other prescribed kits. .. First DNA was cut by restriction enzymes NspI and StyI (Nsp I Enzyme, Sty I Enzyme by New England Biolabs) followed by adaptor ligation (T4 DNA Ligase by New England Biolabs).

    Article Title: North Carolina macular dystrophy (MCDR1) caused by a novel tandem duplication of the PRDM13 gene
    Article Snippet: DNAs from six affected individuals and three unaffected spouses were genotyped at the Hussman Institute for Human Genomics Center for Genome Technology using the Affymetrix Genome-Wide Human SNP Array 6.0 (Santa Clara, CA). .. Briefly, DNA was digested with NspI and StyI restriction enzymes (New England Biolabs, Ipswich, MA), ligated to adapters and amplified using adapter-specific primers.

    Modification:

    Article Title: Molecular typing reveals substantial Plasmodium vivax infection in asymptomatic adults in a rural area of Cameroon
    Article Snippet: Duffy blood group determination To detect the point mutation -33 T → C, which corresponds to a Duffy-negative phenotype, the DARC gene promoter region of 13 subjects were amplified directly from frozen packed red and white cells by PCR, followed by wet lab enzymatic restriction digestion with StyI (New England Biolabs, Ipswich, MA), as well as in silico StyI enzymatic restriction digestion. .. The PCR was done with primer pairs previously published [ ] with a slight modification of the PCR constituents.

    Western Blot:

    Article Title: By Inhibiting Replication, the Large Hepatitis Delta Antigen Can Indirectly Regulate Amber/W Editing and Its Own Expression
    Article Snippet: Paragraph title: Western, Northern, and RT-PCR analyses. ... Ten microliters of the second PCR samples was digested to completion with 20 U of StyI (New England Biolabs) in 1× NEB buffer 3 in a total volume of 20 μl.

    Hybridization:

    Article Title: Genome-wide molecular characterization of central nervous system primitive neuroectodermal tumor and pineoblastoma
    Article Snippet: .. DNA digestion, labeling, and hybridization were performed on the basis of the Affymetrix 100K or 500K SNP array protocols., In brief, 250 ng of DNA was digested (with either Xba1 or HindIII [New England Biolabs] for each 50K SNP array and NspI or StyI [New England Biolabs] for each 250K SNP array). .. After adaptor ligation, samples were amplified by polymerase chain reaction (PCR) using Titanium Taq DNA polymerase (Clontech) for the 100K assay or AmpliTag Gold (Applied Biosystems) for the 500K assay.

    Article Title: North Carolina macular dystrophy (MCDR1) caused by a novel tandem duplication of the PRDM13 gene
    Article Snippet: Briefly, DNA was digested with NspI and StyI restriction enzymes (New England Biolabs, Ipswich, MA), ligated to adapters and amplified using adapter-specific primers. .. Hybridization was performed in a GeneChip Hybridization Oven (Affymetrix, Santa Clara, CA).

    Southern Blot:

    Article Title: A multi-systemic mitochondrial disorder due to a dominant p.Y955H disease variant in DNA polymerase gamma
    Article Snippet: Paragraph title: DNA isolation and southern blot analysis from flies ... Approximately 1–3 µg of total DNA was cut using either XhoI or StyI restriction endonuclease (NEB).

    Ligation:

    Article Title: Genome-wide molecular characterization of central nervous system primitive neuroectodermal tumor and pineoblastoma
    Article Snippet: DNA digestion, labeling, and hybridization were performed on the basis of the Affymetrix 100K or 500K SNP array protocols., In brief, 250 ng of DNA was digested (with either Xba1 or HindIII [New England Biolabs] for each 50K SNP array and NspI or StyI [New England Biolabs] for each 250K SNP array). .. After adaptor ligation, samples were amplified by polymerase chain reaction (PCR) using Titanium Taq DNA polymerase (Clontech) for the 100K assay or AmpliTag Gold (Applied Biosystems) for the 500K assay.

    Article Title: SNPs Array Karyotyping Reveals a Novel Recurrent 20p13 Amplification in Primary Myelofibrosis
    Article Snippet: .. First DNA was cut by restriction enzymes NspI and StyI (Nsp I Enzyme, Sty I Enzyme by New England Biolabs) followed by adaptor ligation (T4 DNA Ligase by New England Biolabs). .. A generic primer that recognizes the adaptor sequence was used to amplify adaptor-ligated DNA fragments (TITANIUM DNA Amplification Kit by Clontech).

    Article Title: A Long Noncoding RNA Controls Muscle Differentiation by Functioning as a Competing Endogenous RNA
    Article Snippet: .. DNA was digested with 800 units of StyI restriction enzyme and ligated in 1X ligation buffer (NEB). .. Ligation products were purified using QIAquick PCR purification kit (QIAGEN).

    SYBR Green Assay:

    Article Title: A multi-systemic mitochondrial disorder due to a dominant p.Y955H disease variant in DNA polymerase gamma
    Article Snippet: Quantification of mtDNA levels was done using SYBR-Green qPCR analyses and primers targeting the mitochondrial encoded genes 12S, COX1 and CytB, and normalized to the nuclear encoded histone gene. .. Approximately 1–3 µg of total DNA was cut using either XhoI or StyI restriction endonuclease (NEB).

    Northern Blot:

    Article Title: By Inhibiting Replication, the Large Hepatitis Delta Antigen Can Indirectly Regulate Amber/W Editing and Its Own Expression
    Article Snippet: Paragraph title: Western, Northern, and RT-PCR analyses. ... Ten microliters of the second PCR samples was digested to completion with 20 U of StyI (New England Biolabs) in 1× NEB buffer 3 in a total volume of 20 μl.

    Infection:

    Article Title: Molecular and Phenotypic Analyses Reveal Association of Diverse Colletotrichum acutatum Groups and a Low Level of C. gloeosporioides with Olive Anthracnose
    Article Snippet: Each of these primers was used in conjunction with the conserved primer TB5 ( ) for diagnostic PCR of C. acutatum and C. gloesoporoides isolates or infected olive samples. .. For the diagnosis of molecular groups within C. acutatum based on unique restriction fragment profiles, the tub2 fragment amplified with primers TB5 and TBCA (specific for C. acutatum ) and TB5 and TB6 (conserved) ( ) were restriction digested using enzymes StyI, NlaIII, RsaI (New England Biolabs, Hitchin, United Kingdom), and SacI (Roche Diagnostics, Mannheim, Germany).

    Generated:

    Article Title: By Inhibiting Replication, the Large Hepatitis Delta Antigen Can Indirectly Regulate Amber/W Editing and Its Own Expression
    Article Snippet: Control PCR samples were also generated in the absence of reverse transcription, and the absence of the 537-bp band was used to verify that DNase I digestion went to completion. .. Ten microliters of the second PCR samples was digested to completion with 20 U of StyI (New England Biolabs) in 1× NEB buffer 3 in a total volume of 20 μl.

    Article Title: Glycine-alanine dipeptide repeat protein contributes to toxicity in a zebrafish model of C9orf72 associated neurodegeneration
    Article Snippet: For the generation of the GA80-GFP and ggggcc80-GFP plasmids were constructed as described in Additional file : Figure S7. pCS2 + eGFP plasmid [ ] was PCR amplified by Phusion high fidelity polymerase (New England Biolabs) using the following primers: GA80-GFP A: pCS2-f1: 5′-ggccgcaGGTGGCGGAGGTGGCGTGAGCAAGGGCGAGGAGC-3′ pCS2-r1: 5′-gCATGGTGGCGGCCTTGGATCCGGAATTCGAATCGATGGGATCCTGCA-3′ B: pCS2-f2: 5′- gcaGGTGGCGGAGGTGGCGTGAGCAAGGGCGAGGAGC-3′ pCS2-r2: 5′- tagCATGGTGGCGGCCTTGGATCCGGAATTCGAATCGATGGGATCCTGCA-3′ ggggcc80xRNA A’: pCS2-f1: 5′- ggccgcaGGTGGCGGAGGTGGCGTGAGCAAGGGCGAGGAGC-3′ pCS2-r3: 5′- gGGTGGCGGCCTTGGATCCGGAATTCGAATCGATGGGATCCTGCA-3′ B’ pCS2-f2: 5′- gcaGGTGGCGGAGGTGGCGTGAGCAAGGGCGAGGAGC-3′ pCS2-r2: 5′- tagCATGGTGGCGGCCTTGGATCCGGAATTCGAATCGATGGGATCCTGCA-3′ After purification of the PCR products generated by A/B or A’/B’, the PCR products were co-incubated at following cycles (94 °C 2 min, 94 °C 30 s, 55 °C 30 s, 72 °C 2 min, 72 °C 10 min, 10 °C 10 min) to produce sticky end fragments digested by NotI/BfaI (New England Biolabs), like for circular plasmid generation. .. The pCS2 + ATG-80xGGGGCC-GFP and pCS2 + 80xGGGGCC plasmids were digested by StyI (New England Biolabs) and HpaI (New England Biolabs) and cloned into pT2KXIGdeltaIN plasmid (a gift from K. Kawakami, National Institute of Genetics, Shizuoka, Japan) to generate transgenic zebrafish by TOL2 mediated transposition.

    Article Title: A Long Noncoding RNA Controls Muscle Differentiation by Functioning as a Competing Endogenous RNA
    Article Snippet: DNA was digested with 800 units of StyI restriction enzyme and ligated in 1X ligation buffer (NEB). .. First, to ensure that primer efficiency does not introduce bias, a control template was generated by digesting and ligating equimolar amounts of all possible PCR products and used to calculate amplification efficiency of each primer pairs.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: By Inhibiting Replication, the Large Hepatitis Delta Antigen Can Indirectly Regulate Amber/W Editing and Its Own Expression
    Article Snippet: Paragraph title: Western, Northern, and RT-PCR analyses. ... Ten microliters of the second PCR samples was digested to completion with 20 U of StyI (New England Biolabs) in 1× NEB buffer 3 in a total volume of 20 μl.

    Binding Assay:

    Article Title: Structural Features of Nucleosomes Reorganized by Yeast FACT and Its HMG Box Component, Nhp6
    Article Snippet: .. DraI, HhaI, and StyI (New England Biolabs) were used in the same way except the NaCl was omitted from the binding reaction and replaced with 1× reaction buffer supplied with the enzyme, and 20 U of each enzyme was added per 100 fmol of DNA. .. Reactions were terminated as above, and digestion was assessed by denaturing PAGE followed by phosphorimaging and analysis of the products with ImageQuant software (Molecular Dynamics).

    Crocin Bleaching Assay:

    Article Title: The Novel Mouse Mutation Oblivion Inactivates the PMCA2 Pump and Causes Progressive Hearing Loss
    Article Snippet: The 111 bp PCR product was digested with StyI (New England Biolabs) which cuts the wildtype allele giving an 89 bp fragment while the mutant allele remains uncut. .. This genotyping tool was used to establish whether the sequence change in Obl DNA was a polymorphism in a total of 17 inbred strains: BALB/C, CBA/Ca, C3HeB/FeJ, DDY/Jc1, 129X1/SvJ, A/J, Bxd-1/Ty, C58/J, CE/J, DA/HuSn, DBA/2J, FL/1Re, LP/J, NON/LtJ, RBG/Dn, St/bJ and SWR/J.

    DNA Extraction:

    Article Title: North Carolina macular dystrophy (MCDR1) caused by a novel tandem duplication of the PRDM13 gene
    Article Snippet: Saliva was collected in Oragene DISCOVER (OGR-500) tubes from DNA Genotek (Ottawa, Canada) and stored at room temperature until DNA extraction could be performed. .. Briefly, DNA was digested with NspI and StyI restriction enzymes (New England Biolabs, Ipswich, MA), ligated to adapters and amplified using adapter-specific primers.

    Article Title: A multi-systemic mitochondrial disorder due to a dominant p.Y955H disease variant in DNA polymerase gamma
    Article Snippet: Paragraph title: DNA isolation and southern blot analysis from flies ... Approximately 1–3 µg of total DNA was cut using either XhoI or StyI restriction endonuclease (NEB).

    Fluorescence:

    Article Title: By Inhibiting Replication, the Large Hepatitis Delta Antigen Can Indirectly Regulate Amber/W Editing and Its Own Expression
    Article Snippet: Ten microliters of the second PCR samples was digested to completion with 20 U of StyI (New England Biolabs) in 1× NEB buffer 3 in a total volume of 20 μl. .. The fluorescence intensity of ethidium bromide-stained bands at 479 bp (unedited) and 393 bp (edited) was quantified by using Kodak Image Station 440CF and ID image analysis software.

    Magnetic Beads:

    Article Title: SNPs Array Karyotyping Reveals a Novel Recurrent 20p13 Amplification in Primary Myelofibrosis
    Article Snippet: First DNA was cut by restriction enzymes NspI and StyI (Nsp I Enzyme, Sty I Enzyme by New England Biolabs) followed by adaptor ligation (T4 DNA Ligase by New England Biolabs). .. PCR products were then purified (Magnetic Beads by Agencourt) fragmented and labeled with a fluorochrome.

    Mutagenesis:

    Article Title: The Novel Mouse Mutation Oblivion Inactivates the PMCA2 Pump and Causes Progressive Hearing Loss
    Article Snippet: .. The 111 bp PCR product was digested with StyI (New England Biolabs) which cuts the wildtype allele giving an 89 bp fragment while the mutant allele remains uncut. .. This genotyping tool was used to establish whether the sequence change in Obl DNA was a polymorphism in a total of 17 inbred strains: BALB/C, CBA/Ca, C3HeB/FeJ, DDY/Jc1, 129X1/SvJ, A/J, Bxd-1/Ty, C58/J, CE/J, DA/HuSn, DBA/2J, FL/1Re, LP/J, NON/LtJ, RBG/Dn, St/bJ and SWR/J.

    Article Title: Glycine-alanine dipeptide repeat protein contributes to toxicity in a zebrafish model of C9orf72 associated neurodegeneration
    Article Snippet: The pCS2 + ATG-80xGGGGCC-GFP and pCS2 + 80xGGGGCC plasmids were digested by StyI (New England Biolabs) and HpaI (New England Biolabs) and cloned into pT2KXIGdeltaIN plasmid (a gift from K. Kawakami, National Institute of Genetics, Shizuoka, Japan) to generate transgenic zebrafish by TOL2 mediated transposition. .. Our pT2KXIGdeltaIN plasmid harbored one point mutation in the UAS region that generated a novel StyI recognition site.

    Article Title: Molecular typing reveals substantial Plasmodium vivax infection in asymptomatic adults in a rural area of Cameroon
    Article Snippet: .. Duffy blood group determination To detect the point mutation -33 T → C, which corresponds to a Duffy-negative phenotype, the DARC gene promoter region of 13 subjects were amplified directly from frozen packed red and white cells by PCR, followed by wet lab enzymatic restriction digestion with StyI (New England Biolabs, Ipswich, MA), as well as in silico StyI enzymatic restriction digestion. .. The PCR was done with primer pairs previously published [ ] with a slight modification of the PCR constituents.

    Isolation:

    Article Title: A Long Noncoding RNA Controls Muscle Differentiation by Functioning as a Competing Endogenous RNA
    Article Snippet: Briefly, chromatin was crosslinked with 1% formaldehyde and nuclei were isolated by using Nonidet P-40. .. DNA was digested with 800 units of StyI restriction enzyme and ligated in 1X ligation buffer (NEB).

    Article Title: Deletion of ETS-1, a gene in the Jacobsen syndrome critical region, causes ventricular septal defects and abnormal ventricular morphology in mice
    Article Snippet: High resolution mapping of the deletion breakpoints was performed using the Affymetrix 500K SNP platform, as described previously ( ) using total genomic isolated DNA isolated from fresh whole blood. .. In short, 250 ng of genomic DNA is digested with 10 units of NspI or StyI (New England Biolabs, Beverly, MA, USA) for 2 h at 37°C.

    Labeling:

    Article Title: Genome-wide molecular characterization of central nervous system primitive neuroectodermal tumor and pineoblastoma
    Article Snippet: .. DNA digestion, labeling, and hybridization were performed on the basis of the Affymetrix 100K or 500K SNP array protocols., In brief, 250 ng of DNA was digested (with either Xba1 or HindIII [New England Biolabs] for each 50K SNP array and NspI or StyI [New England Biolabs] for each 250K SNP array). .. After adaptor ligation, samples were amplified by polymerase chain reaction (PCR) using Titanium Taq DNA polymerase (Clontech) for the 100K assay or AmpliTag Gold (Applied Biosystems) for the 500K assay.

    Article Title: SNPs Array Karyotyping Reveals a Novel Recurrent 20p13 Amplification in Primary Myelofibrosis
    Article Snippet: First DNA was cut by restriction enzymes NspI and StyI (Nsp I Enzyme, Sty I Enzyme by New England Biolabs) followed by adaptor ligation (T4 DNA Ligase by New England Biolabs). .. PCR products were then purified (Magnetic Beads by Agencourt) fragmented and labeled with a fluorochrome.

    Article Title: North Carolina macular dystrophy (MCDR1) caused by a novel tandem duplication of the PRDM13 gene
    Article Snippet: Briefly, DNA was digested with NspI and StyI restriction enzymes (New England Biolabs, Ipswich, MA), ligated to adapters and amplified using adapter-specific primers. .. PCR products were fragmented, labeled and loaded on the SNP 6.0 arrays.

    Purification:

    Article Title: SNPs Array Karyotyping Reveals a Novel Recurrent 20p13 Amplification in Primary Myelofibrosis
    Article Snippet: Briefly, 500 ng of highly purified genomic DNA from each patient was processed in each step by a provided Affymetrix® Genome-Wide Human SNP Nsp/Sty Assay Kit 5.0/6. and other prescribed kits. .. First DNA was cut by restriction enzymes NspI and StyI (Nsp I Enzyme, Sty I Enzyme by New England Biolabs) followed by adaptor ligation (T4 DNA Ligase by New England Biolabs).

    Article Title: Glycine-alanine dipeptide repeat protein contributes to toxicity in a zebrafish model of C9orf72 associated neurodegeneration
    Article Snippet: Subsequently, the 80xGGGGCC fragment was purified and digested by BfaI and NotI. .. The pCS2 + ATG-80xGGGGCC-GFP and pCS2 + 80xGGGGCC plasmids were digested by StyI (New England Biolabs) and HpaI (New England Biolabs) and cloned into pT2KXIGdeltaIN plasmid (a gift from K. Kawakami, National Institute of Genetics, Shizuoka, Japan) to generate transgenic zebrafish by TOL2 mediated transposition.

    Article Title: A Long Noncoding RNA Controls Muscle Differentiation by Functioning as a Competing Endogenous RNA
    Article Snippet: DNA was digested with 800 units of StyI restriction enzyme and ligated in 1X ligation buffer (NEB). .. Ligation products were purified using QIAquick PCR purification kit (QIAGEN).

    Article Title: Deletion of ETS-1, a gene in the Jacobsen syndrome critical region, causes ventricular septal defects and abnormal ventricular morphology in mice
    Article Snippet: In short, 250 ng of genomic DNA is digested with 10 units of NspI or StyI (New England Biolabs, Beverly, MA, USA) for 2 h at 37°C. .. PCR products were purified and fragmented using 0.24 units of DNase I at 37°C for 30 min.

    Sequencing:

    Article Title: SNPs Array Karyotyping Reveals a Novel Recurrent 20p13 Amplification in Primary Myelofibrosis
    Article Snippet: First DNA was cut by restriction enzymes NspI and StyI (Nsp I Enzyme, Sty I Enzyme by New England Biolabs) followed by adaptor ligation (T4 DNA Ligase by New England Biolabs). .. A generic primer that recognizes the adaptor sequence was used to amplify adaptor-ligated DNA fragments (TITANIUM DNA Amplification Kit by Clontech).

    Article Title: The Novel Mouse Mutation Oblivion Inactivates the PMCA2 Pump and Causes Progressive Hearing Loss
    Article Snippet: Sequence traces were analysed using Gap4 software . .. The 111 bp PCR product was digested with StyI (New England Biolabs) which cuts the wildtype allele giving an 89 bp fragment while the mutant allele remains uncut.

    Article Title: Tailoring RNA modular units on a common scaffold: A modular ribozyme with a catalytic unit for ?-nicotinamide mononucleotide-activated RNA ligation
    Article Snippet: Three pool-L oligonucleotides (pool L-1, pool L-2, and pool L-3) including 15, 10, and 5 nt of random sequence, respectively, were amplified by PCR using a sense primer (Fw-L) and an antisense primer (Rv-1). .. Half of each PCR product was digested with BanI (New England Biolabs), and the remaining half was digested with StyI (New England Biolabs).

    Article Title: Glycine-alanine dipeptide repeat protein contributes to toxicity in a zebrafish model of C9orf72 associated neurodegeneration
    Article Snippet: Plasmid construction and generation of transgenic zebrafish For the construction of the GA2-GFP and GA80-GFP plasmids, a Kozak sequence (GCCGCCACC) was inserted 3′ of the ATG. .. The pCS2 + ATG-80xGGGGCC-GFP and pCS2 + 80xGGGGCC plasmids were digested by StyI (New England Biolabs) and HpaI (New England Biolabs) and cloned into pT2KXIGdeltaIN plasmid (a gift from K. Kawakami, National Institute of Genetics, Shizuoka, Japan) to generate transgenic zebrafish by TOL2 mediated transposition.

    Article Title: Deletion of ETS-1, a gene in the Jacobsen syndrome critical region, causes ventricular septal defects and abnormal ventricular morphology in mice
    Article Snippet: In short, 250 ng of genomic DNA is digested with 10 units of NspI or StyI (New England Biolabs, Beverly, MA, USA) for 2 h at 37°C. .. After dilution with water, samples were subjected to PCR using primers specific to the adaptor sequence with the following amplification parameters: 95°C for 3 min (initial denaturation), 95°C for 20 s, 59°C for 15 s, 72°C for 15 s for a total of 35 cycles, followed by 72°C for 7 min (final extension).

    Polyacrylamide Gel Electrophoresis:

    Article Title: Structural Features of Nucleosomes Reorganized by Yeast FACT and Its HMG Box Component, Nhp6
    Article Snippet: Exonuclease III (New England Biolabs) digestions were performed as for DNase I except that various amounts of exonuclease III were added and incubated at 30°C for 10 min before the reaction was terminated and samples were prepared for denaturing PAGE as above. .. DraI, HhaI, and StyI (New England Biolabs) were used in the same way except the NaCl was omitted from the binding reaction and replaced with 1× reaction buffer supplied with the enzyme, and 20 U of each enzyme was added per 100 fmol of DNA.

    Activated Clotting Time Assay:

    Article Title: The Novel Mouse Mutation Oblivion Inactivates the PMCA2 Pump and Causes Progressive Hearing Loss
    Article Snippet: Primers OblRTF ( 5′-CTT CTT CTC CCT GCC ACT GTC GTA G ) and OblRTR ( 5′-CCA CCG AGA CAC CGG TCC CGG TTC ) were used for PCR. .. The 111 bp PCR product was digested with StyI (New England Biolabs) which cuts the wildtype allele giving an 89 bp fragment while the mutant allele remains uncut.

    Mouse Assay:

    Article Title: An axon initial segment is required for temporal precision in action potential encoding by neuronal populations
    Article Snippet: Genotyping Mice were genotyped by polymerase chain reaction (PCR) on tail biopsy samples using the following primers: forward, 5′-AGGCAGCGCCTTTGCTGCGTC-3′; reverse, 5′-TCCTGGTCACAGAGGTCCTTA-3′. .. Enzymatic digestion was performed with StyI (10 U/μl) (New England Biolabs, R0500S).

    Plasmid Preparation:

    Article Title: Glycine-alanine dipeptide repeat protein contributes to toxicity in a zebrafish model of C9orf72 associated neurodegeneration
    Article Snippet: .. The pCS2 + ATG-80xGGGGCC-GFP and pCS2 + 80xGGGGCC plasmids were digested by StyI (New England Biolabs) and HpaI (New England Biolabs) and cloned into pT2KXIGdeltaIN plasmid (a gift from K. Kawakami, National Institute of Genetics, Shizuoka, Japan) to generate transgenic zebrafish by TOL2 mediated transposition. .. Our pT2KXIGdeltaIN plasmid harbored one point mutation in the UAS region that generated a novel StyI recognition site.

    Software:

    Article Title: SNPs Array Karyotyping Reveals a Novel Recurrent 20p13 Amplification in Primary Myelofibrosis
    Article Snippet: CA, U.S.A.) Concentration and quality samples were quantified by Nanodrop ND-1000 spectrophotometer running software version 3.0.1 (NanoDrop Technologies, Inc., Rockland DE). .. First DNA was cut by restriction enzymes NspI and StyI (Nsp I Enzyme, Sty I Enzyme by New England Biolabs) followed by adaptor ligation (T4 DNA Ligase by New England Biolabs).

    Article Title: Structural Features of Nucleosomes Reorganized by Yeast FACT and Its HMG Box Component, Nhp6
    Article Snippet: DraI, HhaI, and StyI (New England Biolabs) were used in the same way except the NaCl was omitted from the binding reaction and replaced with 1× reaction buffer supplied with the enzyme, and 20 U of each enzyme was added per 100 fmol of DNA. .. Reactions were terminated as above, and digestion was assessed by denaturing PAGE followed by phosphorimaging and analysis of the products with ImageQuant software (Molecular Dynamics).

    Article Title: By Inhibiting Replication, the Large Hepatitis Delta Antigen Can Indirectly Regulate Amber/W Editing and Its Own Expression
    Article Snippet: Ten microliters of the second PCR samples was digested to completion with 20 U of StyI (New England Biolabs) in 1× NEB buffer 3 in a total volume of 20 μl. .. The fluorescence intensity of ethidium bromide-stained bands at 479 bp (unedited) and 393 bp (edited) was quantified by using Kodak Image Station 440CF and ID image analysis software.

    Article Title: The Novel Mouse Mutation Oblivion Inactivates the PMCA2 Pump and Causes Progressive Hearing Loss
    Article Snippet: Sequence traces were analysed using Gap4 software . .. The 111 bp PCR product was digested with StyI (New England Biolabs) which cuts the wildtype allele giving an 89 bp fragment while the mutant allele remains uncut.

    Real-time Polymerase Chain Reaction:

    Article Title: A multi-systemic mitochondrial disorder due to a dominant p.Y955H disease variant in DNA polymerase gamma
    Article Snippet: Quantification of mtDNA levels was done using SYBR-Green qPCR analyses and primers targeting the mitochondrial encoded genes 12S, COX1 and CytB, and normalized to the nuclear encoded histone gene. .. Approximately 1–3 µg of total DNA was cut using either XhoI or StyI restriction endonuclease (NEB).

    Selection:

    Article Title: Tailoring RNA modular units on a common scaffold: A modular ribozyme with a catalytic unit for ?-nicotinamide mononucleotide-activated RNA ligation
    Article Snippet: Half of each PCR product was digested with BanI (New England Biolabs), and the remaining half was digested with StyI (New England Biolabs). .. The DNA library for doped selection was also constructed in a similar manner using YFL-1 dope AS and pooled L-3 oligonucleotides as the PCR template to generate 3′ and 5′ fragments, respectively.

    Agarose Gel Electrophoresis:

    Article Title: By Inhibiting Replication, the Large Hepatitis Delta Antigen Can Indirectly Regulate Amber/W Editing and Its Own Expression
    Article Snippet: Ten microliters of the second PCR samples was digested to completion with 20 U of StyI (New England Biolabs) in 1× NEB buffer 3 in a total volume of 20 μl. .. The digest was analyzed by electrophoresis on a 1.5% agarose gel, and the gel was stained with ethidium bromide.

    In Vitro:

    Article Title: Tailoring RNA modular units on a common scaffold: A modular ribozyme with a catalytic unit for ?-nicotinamide mononucleotide-activated RNA ligation
    Article Snippet: Half of each PCR product was digested with BanI (New England Biolabs), and the remaining half was digested with StyI (New England Biolabs). .. The resulting ligated DNAs were used as templates for in vitro transcription with T7 RNA polymerase under standard reaction conditions except the nucleotide composition consisted of 1 mM GTP, UTP, and CTP; 0.1 mM ATP; and 50 mM β-NADH (Sigma).

    Transgenic Assay:

    Article Title: Glycine-alanine dipeptide repeat protein contributes to toxicity in a zebrafish model of C9orf72 associated neurodegeneration
    Article Snippet: .. The pCS2 + ATG-80xGGGGCC-GFP and pCS2 + 80xGGGGCC plasmids were digested by StyI (New England Biolabs) and HpaI (New England Biolabs) and cloned into pT2KXIGdeltaIN plasmid (a gift from K. Kawakami, National Institute of Genetics, Shizuoka, Japan) to generate transgenic zebrafish by TOL2 mediated transposition. .. Our pT2KXIGdeltaIN plasmid harbored one point mutation in the UAS region that generated a novel StyI recognition site.

    Quantitation Assay:

    Article Title: By Inhibiting Replication, the Large Hepatitis Delta Antigen Can Indirectly Regulate Amber/W Editing and Its Own Expression
    Article Snippet: If depletion were to occur, heteroduplexes between edited and unedited products could form, such species would not be digested by StyI, and a quantitation error would result. .. Ten microliters of the second PCR samples was digested to completion with 20 U of StyI (New England Biolabs) in 1× NEB buffer 3 in a total volume of 20 μl.

    Spectrophotometry:

    Article Title: SNPs Array Karyotyping Reveals a Novel Recurrent 20p13 Amplification in Primary Myelofibrosis
    Article Snippet: CA, U.S.A.) Concentration and quality samples were quantified by Nanodrop ND-1000 spectrophotometer running software version 3.0.1 (NanoDrop Technologies, Inc., Rockland DE). .. First DNA was cut by restriction enzymes NspI and StyI (Nsp I Enzyme, Sty I Enzyme by New England Biolabs) followed by adaptor ligation (T4 DNA Ligase by New England Biolabs).

    Concentration Assay:

    Article Title: SNPs Array Karyotyping Reveals a Novel Recurrent 20p13 Amplification in Primary Myelofibrosis
    Article Snippet: CA, U.S.A.) Concentration and quality samples were quantified by Nanodrop ND-1000 spectrophotometer running software version 3.0.1 (NanoDrop Technologies, Inc., Rockland DE). .. First DNA was cut by restriction enzymes NspI and StyI (Nsp I Enzyme, Sty I Enzyme by New England Biolabs) followed by adaptor ligation (T4 DNA Ligase by New England Biolabs).

    Marker:

    Article Title: SNPs Array Karyotyping Reveals a Novel Recurrent 20p13 Amplification in Primary Myelofibrosis
    Article Snippet: DNA was then treated as far manufacturer's instructions (Affymetrix, Santa Clara, CA, USA) and finally hybridized to the Genome-Wide SNP array 6.0 (Affymetrix, Santa Clara, CA, USA).This array contains 906,600 Single Nucleotide Polymorphism (SNP) probes and more than 945,826 copy number variant (CNV) probes, providing marker spacing in the range of as low as 700 bases. .. First DNA was cut by restriction enzymes NspI and StyI (Nsp I Enzyme, Sty I Enzyme by New England Biolabs) followed by adaptor ligation (T4 DNA Ligase by New England Biolabs).

    Staining:

    Article Title: By Inhibiting Replication, the Large Hepatitis Delta Antigen Can Indirectly Regulate Amber/W Editing and Its Own Expression
    Article Snippet: Ten microliters of the second PCR samples was digested to completion with 20 U of StyI (New England Biolabs) in 1× NEB buffer 3 in a total volume of 20 μl. .. The digest was analyzed by electrophoresis on a 1.5% agarose gel, and the gel was stained with ethidium bromide.

    Article Title: North Carolina macular dystrophy (MCDR1) caused by a novel tandem duplication of the PRDM13 gene
    Article Snippet: Briefly, DNA was digested with NspI and StyI restriction enzymes (New England Biolabs, Ipswich, MA), ligated to adapters and amplified using adapter-specific primers. .. Arrays were washed and stained with streptavidin phycoerythrin and scanned on a GeneChip Scanner 3000 7G (Affymetrix).

    Variant Assay:

    Article Title: SNPs Array Karyotyping Reveals a Novel Recurrent 20p13 Amplification in Primary Myelofibrosis
    Article Snippet: DNA was then treated as far manufacturer's instructions (Affymetrix, Santa Clara, CA, USA) and finally hybridized to the Genome-Wide SNP array 6.0 (Affymetrix, Santa Clara, CA, USA).This array contains 906,600 Single Nucleotide Polymorphism (SNP) probes and more than 945,826 copy number variant (CNV) probes, providing marker spacing in the range of as low as 700 bases. .. First DNA was cut by restriction enzymes NspI and StyI (Nsp I Enzyme, Sty I Enzyme by New England Biolabs) followed by adaptor ligation (T4 DNA Ligase by New England Biolabs).

    Fluorescence In Situ Hybridization:

    Article Title: Glycine-alanine dipeptide repeat protein contributes to toxicity in a zebrafish model of C9orf72 associated neurodegeneration
    Article Snippet: To prepare the gggggcc80-GFP plasmid for the generation of transgenic fish, the pEF-80xGGGGCC plasmid [ ] was digested by BamHI and PmeI (New England Biolabs). .. The pCS2 + ATG-80xGGGGCC-GFP and pCS2 + 80xGGGGCC plasmids were digested by StyI (New England Biolabs) and HpaI (New England Biolabs) and cloned into pT2KXIGdeltaIN plasmid (a gift from K. Kawakami, National Institute of Genetics, Shizuoka, Japan) to generate transgenic zebrafish by TOL2 mediated transposition.

    In Silico:

    Article Title: Molecular typing reveals substantial Plasmodium vivax infection in asymptomatic adults in a rural area of Cameroon
    Article Snippet: .. Duffy blood group determination To detect the point mutation -33 T → C, which corresponds to a Duffy-negative phenotype, the DARC gene promoter region of 13 subjects were amplified directly from frozen packed red and white cells by PCR, followed by wet lab enzymatic restriction digestion with StyI (New England Biolabs, Ipswich, MA), as well as in silico StyI enzymatic restriction digestion. .. The PCR was done with primer pairs previously published [ ] with a slight modification of the PCR constituents.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90
    New England Biolabs styi restriction endonuclease
    Styi Restriction Endonuclease, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/styi restriction endonuclease/product/New England Biolabs
    Average 90 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    styi restriction endonuclease - by Bioz Stars, 2020-03
    90/100 stars
      Buy from Supplier

    Image Search Results