ncii  (New England Biolabs)


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    New England Biolabs ncii
    Conversion of iPSCs from Pl A1 to Pl A2 . (A) Genomic DNA, isolated from iPSCs that had been transfected with px462-gRNA1, px462-gRNA2, and Pl A2 -ssODN, was <t>PCR</t> amplified and digested with <t>NciI,</t> which differentiates the Pl A1 allelic isoform from Pl A2 . Red
    Ncii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ncii/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ncii - by Bioz Stars, 2022-05
    94/100 stars

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    1) Product Images from "CRISPR/Cas9-mediated conversion of human platelet alloantigen allotypes"

    Article Title: CRISPR/Cas9-mediated conversion of human platelet alloantigen allotypes

    Journal: Blood

    doi: 10.1182/blood-2015-10-675751

    Conversion of iPSCs from Pl A1 to Pl A2 . (A) Genomic DNA, isolated from iPSCs that had been transfected with px462-gRNA1, px462-gRNA2, and Pl A2 -ssODN, was PCR amplified and digested with NciI, which differentiates the Pl A1 allelic isoform from Pl A2 . Red
    Figure Legend Snippet: Conversion of iPSCs from Pl A1 to Pl A2 . (A) Genomic DNA, isolated from iPSCs that had been transfected with px462-gRNA1, px462-gRNA2, and Pl A2 -ssODN, was PCR amplified and digested with NciI, which differentiates the Pl A1 allelic isoform from Pl A2 . Red

    Techniques Used: Isolation, Transfection, Polymerase Chain Reaction, Amplification

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    New England Biolabs nci i
    Gene targeting of zebrafish rh1-1 . A : Schematic representation of the zebrafish rh1-1 locus characterized by a single exon encoding 354 amino acid proteins and 5′ and 3′ UTRs. B : Alignment of Rho N- and C-terminal amino acid sequences across species. Numbering is based on the predicted zebrafish rh1–1 . The conserved N-linked glycosylation sequence and VXPX targeting sequence are underlined. C : DNA sequence and amino acid overlay of zebrafish rh1–1 5′- (left) and 3′- (right) coding sequences. Blue text represents the CRISPR PAM sequence. Underlined DNA represents the target for guide RNA hybridization. The purple overscore represent restriction sites for <t>Nci</t> I or Bbs I. Arrows indicate predicted Cas9 cleavage sites. D : Diagrammatic representation of the 253-bp 5′- rh1–1 gene amplicon and the predicted products following digestion with Nci I. E : RFLP analysis of a 253-bp PCR product spanning the 5′ rh1–1 gene (C, undigested control) and digested with Nci I (N) shows retention of the original amplicon (arrow) from pooled DNA from injected embryos versus complete digestion of the PCR product of uninjected control DNA (arrowhead). F : Diagrammatic representation of the 325-bp 3′-amplicon and the predicted products following digestion with Bbs I. G : RFLP analysis of a 325-bp PCR product (C, undigested control) spanning the 3′ rh1–1 sequence digested with Bbs I (B). The PCR product (arrow) from uninjected-controls was digested to near completion, yielding two visible bands of 102 and 182 bp. The 284-bp band (arrowhead) following loss of the 5′- Bbs I site is clearly visible in the injected embryos relative to the controls. The following sequences were used for the RHO alignment: Danio rerio ( NP571159.1 ), X. laevis ( NP001080517.1 ), Mus musculus ( NP663358.1 ), Bos taurus ( NP001014890.1 ), and Homo sapiens ( NP000530.1 ).
    Nci I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nci i/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nci i - by Bioz Stars, 2022-05
    94/100 stars
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    Gene targeting of zebrafish rh1-1 . A : Schematic representation of the zebrafish rh1-1 locus characterized by a single exon encoding 354 amino acid proteins and 5′ and 3′ UTRs. B : Alignment of Rho N- and C-terminal amino acid sequences across species. Numbering is based on the predicted zebrafish rh1–1 . The conserved N-linked glycosylation sequence and VXPX targeting sequence are underlined. C : DNA sequence and amino acid overlay of zebrafish rh1–1 5′- (left) and 3′- (right) coding sequences. Blue text represents the CRISPR PAM sequence. Underlined DNA represents the target for guide RNA hybridization. The purple overscore represent restriction sites for Nci I or Bbs I. Arrows indicate predicted Cas9 cleavage sites. D : Diagrammatic representation of the 253-bp 5′- rh1–1 gene amplicon and the predicted products following digestion with Nci I. E : RFLP analysis of a 253-bp PCR product spanning the 5′ rh1–1 gene (C, undigested control) and digested with Nci I (N) shows retention of the original amplicon (arrow) from pooled DNA from injected embryos versus complete digestion of the PCR product of uninjected control DNA (arrowhead). F : Diagrammatic representation of the 325-bp 3′-amplicon and the predicted products following digestion with Bbs I. G : RFLP analysis of a 325-bp PCR product (C, undigested control) spanning the 3′ rh1–1 sequence digested with Bbs I (B). The PCR product (arrow) from uninjected-controls was digested to near completion, yielding two visible bands of 102 and 182 bp. The 284-bp band (arrowhead) following loss of the 5′- Bbs I site is clearly visible in the injected embryos relative to the controls. The following sequences were used for the RHO alignment: Danio rerio ( NP571159.1 ), X. laevis ( NP001080517.1 ), Mus musculus ( NP663358.1 ), Bos taurus ( NP001014890.1 ), and Homo sapiens ( NP000530.1 ).

    Journal: Molecular Vision

    Article Title: Targeted disruption of the endogenous zebrafish rhodopsin locus as models of rapid rod photoreceptor degeneration

    doi:

    Figure Lengend Snippet: Gene targeting of zebrafish rh1-1 . A : Schematic representation of the zebrafish rh1-1 locus characterized by a single exon encoding 354 amino acid proteins and 5′ and 3′ UTRs. B : Alignment of Rho N- and C-terminal amino acid sequences across species. Numbering is based on the predicted zebrafish rh1–1 . The conserved N-linked glycosylation sequence and VXPX targeting sequence are underlined. C : DNA sequence and amino acid overlay of zebrafish rh1–1 5′- (left) and 3′- (right) coding sequences. Blue text represents the CRISPR PAM sequence. Underlined DNA represents the target for guide RNA hybridization. The purple overscore represent restriction sites for Nci I or Bbs I. Arrows indicate predicted Cas9 cleavage sites. D : Diagrammatic representation of the 253-bp 5′- rh1–1 gene amplicon and the predicted products following digestion with Nci I. E : RFLP analysis of a 253-bp PCR product spanning the 5′ rh1–1 gene (C, undigested control) and digested with Nci I (N) shows retention of the original amplicon (arrow) from pooled DNA from injected embryos versus complete digestion of the PCR product of uninjected control DNA (arrowhead). F : Diagrammatic representation of the 325-bp 3′-amplicon and the predicted products following digestion with Bbs I. G : RFLP analysis of a 325-bp PCR product (C, undigested control) spanning the 3′ rh1–1 sequence digested with Bbs I (B). The PCR product (arrow) from uninjected-controls was digested to near completion, yielding two visible bands of 102 and 182 bp. The 284-bp band (arrowhead) following loss of the 5′- Bbs I site is clearly visible in the injected embryos relative to the controls. The following sequences were used for the RHO alignment: Danio rerio ( NP571159.1 ), X. laevis ( NP001080517.1 ), Mus musculus ( NP663358.1 ), Bos taurus ( NP001014890.1 ), and Homo sapiens ( NP000530.1 ).

    Article Snippet: For the restriction fragment length polymorphism (RFLP) analysis of 5′ rh1-1 , a 253-bp product was amplified (forward primer: 5′ ACA GTC CTG CCC AGA CAT CTA 3′; reverse primer: 5′ ATG GTG ACG TAC AGC GTG AG 3′) and digested with Nci I ( New England Biolabs , Ipswich, MA).

    Techniques: Sequencing, CRISPR, Hybridization, Amplification, Polymerase Chain Reaction, Injection

    Conversion of iPSCs from Pl A1 to Pl A2 . (A) Genomic DNA, isolated from iPSCs that had been transfected with px462-gRNA1, px462-gRNA2, and Pl A2 -ssODN, was PCR amplified and digested with NciI, which differentiates the Pl A1 allelic isoform from Pl A2 . Red

    Journal: Blood

    Article Title: CRISPR/Cas9-mediated conversion of human platelet alloantigen allotypes

    doi: 10.1182/blood-2015-10-675751

    Figure Lengend Snippet: Conversion of iPSCs from Pl A1 to Pl A2 . (A) Genomic DNA, isolated from iPSCs that had been transfected with px462-gRNA1, px462-gRNA2, and Pl A2 -ssODN, was PCR amplified and digested with NciI, which differentiates the Pl A1 allelic isoform from Pl A2 . Red

    Article Snippet: PCR products were purified using QiaQuick Spin Column, digested with NciI (New England Biolabs Inc., Ipswich, MA) and analyzed on 2% agarose gels.

    Techniques: Isolation, Transfection, Polymerase Chain Reaction, Amplification

    Presence of the mutation within the index family. A: Pedigree of the family, with the filled symbols representing the affected members and the proband indicated by an arrow . B: Restriction analysis with Nci I; mutation present in the mother and daughter

    Journal:

    Article Title: A Novel Missense Mutation in the Second Extracellular Domain of GJB2, p.Ser183Phe, Causes a Syndrome of Focal Palmoplantar Keratoderma with Deafness

    doi: 10.2353/ajpath.2008.080049

    Figure Lengend Snippet: Presence of the mutation within the index family. A: Pedigree of the family, with the filled symbols representing the affected members and the proband indicated by an arrow . B: Restriction analysis with Nci I; mutation present in the mother and daughter

    Article Snippet: The mutation eliminates an Nci I (New England Biolabs, Frankfurt am Main, Germany) restriction site (the wild type contains two Nci I sites).

    Techniques: Mutagenesis