noti restriction enzymes  (New England Biolabs)

Journal: Journal of Controlled Release

Article Title: EphA2 targeting peptide tethered bioreducible poly(cystamine bisacrylamide - diamino hexane) for the delivery of therapeutic pCMV-RAE-1? to pancreatic islets

doi: 10.1016/j.jconrel.2011.10.022

Figure Lengend Snippet: Digestion of pCMV-RAE-1γ with KpnI and NotI.

Article Snippet: KpnI and NotI restriction enzymes were obtained from New England Biolabs (Ipswich, MA).

Techniques:

Journal: Cell

Article Title: Positional Cloning of the Mouse Circadian Clock Gene

doi:

Figure Lengend Snippet: Genetic, Physical, and Transcription Unit Mapping of the Clock Locus (A) Genetic map of mouse chromosome 5, showing the location of Clock on the midportion of this chromosome, 0.7 cM distal of Kit , flanked by D5Mit307 and D5Mit112 . (B) Physical and high resolution genetic mapping of the Clock locus. The SSLPs and STSs in the D5Mit307 –D5Mit112 interval are shown on the upper bar. The maximum nonrecombinant interval is defined by the SSLPs D5Mit307 and D5Nwu2 . The animals with recombinations between these SSLPs and Clock are noted. Also shown are restriction sites for NotI (depicted with an [N]), which identify two CpG islands associated with the 5′ ends of Clock and pFT27 . YAC and BAC contigs of the Clock . The BAC clones were isolated from a library constructed in Dr. Melvin Simon’s laboratory. (C) Candidate genes mapping to the nonrecombinant interval. Shown are their relative locations and transcriptional orientations.

Article Snippet: After NotI digestion and separation by FIGE, BAC insert DNA was purified by i -agarase digestion (New England Biolabs).

Techniques: BAC Assay, Clone Assay, Isolation, Construct

Journal: Applied and Environmental Microbiology

Article Title: Transmission of Yersinia pseudotuberculosis in the Pork Production Chain from Farm to Slaughterhouse ▿

doi: 10.1128/AEM.02664-07

Figure Lengend Snippet: Four different NotI profiles (lanes 1 to 4) of Yersinia pseudotuberculosis strains obtained. M designates midrange PFGE markers.

Article Snippet: A total of 286 isolates were characterized by pulsed-field gel electrophoresis (PFGE) as described by Niskanen et al. ( ) using SpeI and NotI restriction enzymes (New England Biolabs, Ipswich, MA).

Techniques:

Journal: PLoS Neglected Tropical Diseases

Article Title: Isolation and Characterization of Two Novel Plasmids from Pathogenic Leptospira interrogans Serogroup Canicola Serovar Canicola Strain Gui44

doi: 10.1371/journal.pntd.0003103

Figure Lengend Snippet: Characterization of pGui1 and pGui2 in the pathogenic L.interrogans strain Gui44 by PFGE and Southern blot. PFGE and Southern Blot (using radiolabeled spGui1 probe) of undigested plasmid DNA (lanes A1 and B1); plasmid DNA digested with NotI (A2 and B2); plasmid DNA digested with BamHI (A3 and B3); total genomic DNA digested with NotI (A4 and B4); and undigested total genomic DNA (A5 and B5). The fragment in A2 and B2 indicates the linearized pGui1 (∼78 kb). The corresponding fragment presents in A4 and B4. The larger fragment in A3 indicates the linearized pGui2. The smaller fragment in A4 is one fragment of pGui1 digested with BamHI. Salmonella Braenderup (H9812) digested with XbaI and is used as the molecular weight size standard (M).

Article Snippet: The plugs were then washed twice with pre-warmed TE buffer, and then digested with either restriction enzyme NotI or BamHI at 37°C or 30°C, respectively, following manufacturer's instructions (New England Biolabs, Ipswich, MA, USA).

Techniques: Southern Blot, Plasmid Preparation, Molecular Weight