noti restriction enzymes  (New England Biolabs)


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    Name:
    NotI
    Description:
    NotI 2 500 units
    Catalog Number:
    r0189l
    Price:
    302
    Size:
    2 500 units
    Category:
    Restriction Enzymes
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    Structured Review

    New England Biolabs noti restriction enzymes
    NotI
    NotI 2 500 units
    https://www.bioz.com/result/noti restriction enzymes/product/New England Biolabs
    Average 97 stars, based on 163 article reviews
    Price from $9.99 to $1999.99
    noti restriction enzymes - by Bioz Stars, 2020-05
    97/100 stars

    Images

    1) Product Images from "EphA2 targeting peptide tethered bioreducible poly(cystamine bisacrylamide - diamino hexane) for the delivery of therapeutic pCMV-RAE-1? to pancreatic islets"

    Article Title: EphA2 targeting peptide tethered bioreducible poly(cystamine bisacrylamide - diamino hexane) for the delivery of therapeutic pCMV-RAE-1? to pancreatic islets

    Journal: Journal of Controlled Release

    doi: 10.1016/j.jconrel.2011.10.022

    Digestion of pCMV-RAE-1γ with KpnI and NotI.
    Figure Legend Snippet: Digestion of pCMV-RAE-1γ with KpnI and NotI.

    Techniques Used:

    2) Product Images from "Positional Cloning of the Mouse Circadian Clock Gene"

    Article Title: Positional Cloning of the Mouse Circadian Clock Gene

    Journal: Cell

    doi:

    Genetic, Physical, and Transcription Unit Mapping of the Clock Locus (A) Genetic map of mouse chromosome 5, showing the location of Clock on the midportion of this chromosome, 0.7 cM distal of Kit , flanked by D5Mit307 and D5Mit112 . (B) Physical and high resolution genetic mapping of the Clock locus. The SSLPs and STSs in the D5Mit307 –D5Mit112 interval are shown on the upper bar. The maximum nonrecombinant interval is defined by the SSLPs D5Mit307 and D5Nwu2 . The animals with recombinations between these SSLPs and Clock are noted. Also shown are restriction sites for NotI (depicted with an [N]), which identify two CpG islands associated with the 5′ ends of Clock and pFT27 . YAC and BAC contigs of the Clock . The BAC clones were isolated from a library constructed in Dr. Melvin Simon’s laboratory. (C) Candidate genes mapping to the nonrecombinant interval. Shown are their relative locations and transcriptional orientations.
    Figure Legend Snippet: Genetic, Physical, and Transcription Unit Mapping of the Clock Locus (A) Genetic map of mouse chromosome 5, showing the location of Clock on the midportion of this chromosome, 0.7 cM distal of Kit , flanked by D5Mit307 and D5Mit112 . (B) Physical and high resolution genetic mapping of the Clock locus. The SSLPs and STSs in the D5Mit307 –D5Mit112 interval are shown on the upper bar. The maximum nonrecombinant interval is defined by the SSLPs D5Mit307 and D5Nwu2 . The animals with recombinations between these SSLPs and Clock are noted. Also shown are restriction sites for NotI (depicted with an [N]), which identify two CpG islands associated with the 5′ ends of Clock and pFT27 . YAC and BAC contigs of the Clock . The BAC clones were isolated from a library constructed in Dr. Melvin Simon’s laboratory. (C) Candidate genes mapping to the nonrecombinant interval. Shown are their relative locations and transcriptional orientations.

    Techniques Used: BAC Assay, Clone Assay, Isolation, Construct

    3) Product Images from "Transmission of Yersinia pseudotuberculosis in the Pork Production Chain from Farm to Slaughterhouse ▿"

    Article Title: Transmission of Yersinia pseudotuberculosis in the Pork Production Chain from Farm to Slaughterhouse ▿

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.02664-07

    Four different NotI profiles (lanes 1 to 4) of Yersinia pseudotuberculosis strains obtained. M designates midrange PFGE markers.
    Figure Legend Snippet: Four different NotI profiles (lanes 1 to 4) of Yersinia pseudotuberculosis strains obtained. M designates midrange PFGE markers.

    Techniques Used:

    4) Product Images from "The production of KIR-Fc fusion proteins and their use in a multiplex HLA class I binding assay"

    Article Title: The production of KIR-Fc fusion proteins and their use in a multiplex HLA class I binding assay

    Journal: Journal of immunological methods

    doi: 10.1016/j.jim.2015.06.012

    (A) Schematic diagram showing the configuration of a recombinant KIR-Fc fusion gene. The recombinant fusion gene consisting of the D1, D2 and stem domains of a KIR2D molecule (grey box) and the Fc region of a human IgG1 antibody (white box) is cloned
    Figure Legend Snippet: (A) Schematic diagram showing the configuration of a recombinant KIR-Fc fusion gene. The recombinant fusion gene consisting of the D1, D2 and stem domains of a KIR2D molecule (grey box) and the Fc region of a human IgG1 antibody (white box) is cloned

    Techniques Used: Recombinant, Clone Assay

    5) Product Images from "Disruption of the Rag-Ragulator complex by c17orf59 inhibits mTORC1"

    Article Title: Disruption of the Rag-Ragulator complex by c17orf59 inhibits mTORC1

    Journal: Cell reports

    doi: 10.1016/j.celrep.2015.07.052

    c17orf59 localizes to lysosomes with Ragulator
    Figure Legend Snippet: c17orf59 localizes to lysosomes with Ragulator

    Techniques Used:

    Overexpression of c17orf59 inhibits mTORC1
    Figure Legend Snippet: Overexpression of c17orf59 inhibits mTORC1

    Techniques Used: Over Expression

    c17orf59 is a Ragulator-interacting protein
    Figure Legend Snippet: c17orf59 is a Ragulator-interacting protein

    Techniques Used:

    Loss of c17orf59 does not alter mTORC1 signaling in response to amino acids or insulin
    Figure Legend Snippet: Loss of c17orf59 does not alter mTORC1 signaling in response to amino acids or insulin

    Techniques Used:

    c17orf59 disrupts the Rag-Ragulator interaction
    Figure Legend Snippet: c17orf59 disrupts the Rag-Ragulator interaction

    Techniques Used:

    6) Product Images from "MicroRNA-143 and -145 modulate the phenotype of synovial fibroblasts in rheumatoid arthritis"

    Article Title: MicroRNA-143 and -145 modulate the phenotype of synovial fibroblasts in rheumatoid arthritis

    Journal: Experimental & Molecular Medicine

    doi: 10.1038/emm.2017.108

    miR-143/145-target prediction and network model of the predicted targets in RA-FLSs. ( a ) Heatmap of 470 DEGs up- or downregulated in RA-FLSs irrespective of IL-1β stimulation. Compared profile sets were as follows: RA-FLSs versus OA-FLSs; RA-FLSs with IL-1β versus OA-FLSs with IL-1β RA-FLSs with IL-1β versus RA-FLSs; OA-FLSs with IL-1β versus OA-FLSs. Cluster 1 is a group of 98 DEGs that was upregulated in RA-FLSs and Cluster 2 represents 135 DEGs that were downregulated. The numbers in parenthesis refer to the number of included DEGs in each cluster. Red and blue denote high and low expression of DEGs, respectively ( b ) Venn diagram showing the relationship between two sets of predicted target genes for miR-143 and miR-145 and two DEG cluster sets of RA-FLSs versus OA-FLSs. The numbers of included genes are represented. ( c ) Network model of predicted target genes for miR-143 and miR-145 (miR-143: 404 genes, miR-145: 731 genes, shared: 54 genes, and total: 1081 genes). Node color refers to fold change of RA-FLSs versus OA-FLSs (red=up, cyan=down). Node size refers to -log10P of RA-FLSs versus OA-FLSs. Edge thickness indicates edge betweenness (upper panel). The table in the lower panel shows the fold change, probability of conserved targeting (PCT) score, and the degree of interactions of potential target gene candidates in the network model. ( d , e ) Relative expression of six selected target DEGs in RA-FLSs transfected with miR-143 siRNA ( d ) or miR-145 siRNA ( e ), as determined by real-time PCR. GAPDH mRNA was used as an internal control. The data show the mean±s.d. * P
    Figure Legend Snippet: miR-143/145-target prediction and network model of the predicted targets in RA-FLSs. ( a ) Heatmap of 470 DEGs up- or downregulated in RA-FLSs irrespective of IL-1β stimulation. Compared profile sets were as follows: RA-FLSs versus OA-FLSs; RA-FLSs with IL-1β versus OA-FLSs with IL-1β RA-FLSs with IL-1β versus RA-FLSs; OA-FLSs with IL-1β versus OA-FLSs. Cluster 1 is a group of 98 DEGs that was upregulated in RA-FLSs and Cluster 2 represents 135 DEGs that were downregulated. The numbers in parenthesis refer to the number of included DEGs in each cluster. Red and blue denote high and low expression of DEGs, respectively ( b ) Venn diagram showing the relationship between two sets of predicted target genes for miR-143 and miR-145 and two DEG cluster sets of RA-FLSs versus OA-FLSs. The numbers of included genes are represented. ( c ) Network model of predicted target genes for miR-143 and miR-145 (miR-143: 404 genes, miR-145: 731 genes, shared: 54 genes, and total: 1081 genes). Node color refers to fold change of RA-FLSs versus OA-FLSs (red=up, cyan=down). Node size refers to -log10P of RA-FLSs versus OA-FLSs. Edge thickness indicates edge betweenness (upper panel). The table in the lower panel shows the fold change, probability of conserved targeting (PCT) score, and the degree of interactions of potential target gene candidates in the network model. ( d , e ) Relative expression of six selected target DEGs in RA-FLSs transfected with miR-143 siRNA ( d ) or miR-145 siRNA ( e ), as determined by real-time PCR. GAPDH mRNA was used as an internal control. The data show the mean±s.d. * P

    Techniques Used: Expressing, Transfection, Real-time Polymerase Chain Reaction

    Validation of IGFBP5 and SEMA3A as miR-143 and miR-145 targets, respectively. ( a ) Comparison of IGFBP5 mRNA expression between RA-FLSs ( n =7) and OA-FLSs ( n =10), which was determined by real-time PCR. GAPDH mRNA was used as an internal control. miR-143 HIGH ( n =3) and miR-143 LOW ( n =4) indicate RA-FLS subsets with higher and lower expression of miR-143, respectively. ( b ) Negative correlation between miR-143 and IGFBP5 expression in RA-FLSs ( n =7). ( c ) Negative correlation of IGFBP5 gene expression levels with eight TFGβ-responsive genes in RA-FLSs ( n =19) used in the previous study by Kasperkovitz et al. 19 (GSE4061) and those ( n =6) in this study (GSE22956 and GSE49604). ACTA2 , Actin, Alpha 2, Smooth Muscle, Aorta; COL3A1 , Collagen Type III Alpha 1 Chain; COL4A2 , Collagen Type IV Alpha 2 Chain; POSTN , Periostin; SPARC , secreted protein acidic and rich in cysteine; CYR61 , Cysteine Rich Angiogenic Inducer 61; SERPINH1 , Serine (or Cysteine) Proteinase Inhibitor, Clade H (Heat Shock Protein 47), Member 1 (Collagen Binding Protein 1); CALD1 , Caldesmon 1. Values are Pearson’s rho coefficients. ( d ) SEMA3A mRNA expression in RA-FLSs ( n =7) versus OA-FLSs ( n =10). miR-145 HIGH ( n =3) and miR-145 LOW ( n =4) indicate RA-FLS subsets with higher and lower expression of miR-145, respectively. ( e ) Sequence of miR-145 seed regions in the SEMA3A 3′-UTR. The mutation sequences are indicated in blue. ( f ) Relative normalized Renilla luciferase activity of reporter vector containing putative miR-145 binding sites (WT) or its mutated version (MUT). HEK293T cells were transfected with reporter vectors in the presence or absence of control mimic or synthetic miR-145 miRNA mimic. Single mutants of two putative miR-145-binding sites (seed regions) are indicated as WT-MUT or MUT-WT. A double mutant is denoted as MUT-MUT. RLU, relative light unit. The data show the mean±s.d. of three independent experiments. * P
    Figure Legend Snippet: Validation of IGFBP5 and SEMA3A as miR-143 and miR-145 targets, respectively. ( a ) Comparison of IGFBP5 mRNA expression between RA-FLSs ( n =7) and OA-FLSs ( n =10), which was determined by real-time PCR. GAPDH mRNA was used as an internal control. miR-143 HIGH ( n =3) and miR-143 LOW ( n =4) indicate RA-FLS subsets with higher and lower expression of miR-143, respectively. ( b ) Negative correlation between miR-143 and IGFBP5 expression in RA-FLSs ( n =7). ( c ) Negative correlation of IGFBP5 gene expression levels with eight TFGβ-responsive genes in RA-FLSs ( n =19) used in the previous study by Kasperkovitz et al. 19 (GSE4061) and those ( n =6) in this study (GSE22956 and GSE49604). ACTA2 , Actin, Alpha 2, Smooth Muscle, Aorta; COL3A1 , Collagen Type III Alpha 1 Chain; COL4A2 , Collagen Type IV Alpha 2 Chain; POSTN , Periostin; SPARC , secreted protein acidic and rich in cysteine; CYR61 , Cysteine Rich Angiogenic Inducer 61; SERPINH1 , Serine (or Cysteine) Proteinase Inhibitor, Clade H (Heat Shock Protein 47), Member 1 (Collagen Binding Protein 1); CALD1 , Caldesmon 1. Values are Pearson’s rho coefficients. ( d ) SEMA3A mRNA expression in RA-FLSs ( n =7) versus OA-FLSs ( n =10). miR-145 HIGH ( n =3) and miR-145 LOW ( n =4) indicate RA-FLS subsets with higher and lower expression of miR-145, respectively. ( e ) Sequence of miR-145 seed regions in the SEMA3A 3′-UTR. The mutation sequences are indicated in blue. ( f ) Relative normalized Renilla luciferase activity of reporter vector containing putative miR-145 binding sites (WT) or its mutated version (MUT). HEK293T cells were transfected with reporter vectors in the presence or absence of control mimic or synthetic miR-145 miRNA mimic. Single mutants of two putative miR-145-binding sites (seed regions) are indicated as WT-MUT or MUT-WT. A double mutant is denoted as MUT-MUT. RLU, relative light unit. The data show the mean±s.d. of three independent experiments. * P

    Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Binding Assay, Sequencing, Mutagenesis, Luciferase, Activity Assay, Plasmid Preparation, Transfection

    microRNA profiling in RA-FLSs. ( a ) Heatmap of microarray data showing DEmiRs among RA-FLSs versus OA-FLSs, RA-FLSs with IL-1β versus RA-FLSs, OA-FLSs with IL-1β versus OA-FLSs and RA-FLSs with IL-1β versus OA-FLSs with IL-1β. Red and green denote high and low expression of DEmiRs, respectively; red and green denote high and low expression of DEGs, respectively. ( b ) Higher levels of miR-143 and miR-145 in RA-FLSs ( n =5) than in OA-FLSs ( n =5) as determined by quantitative real-time PCR; 5.6-fold, P =0.031 for miR-143; 2.6-fold, P =0.028 for miR-145. RUN6B_2 small nuclear RNA (snRNA) was used as an internal control. ( c ) Spearman correlation of relative expression of miR-143 and miR-145 in RA-FLSs ( n =8). ( d ) miR-143 and miR-145 levels in synovial fluids obtained from RA patients (RA-SF, n =4) and OA patients (OA-SF, n =5) as determined by real-time PCR. ( e ) Increase in miR-145 expression in FLSs by TGFβ stimulation. The OA-FLSs ( n =4) and RA-FLSs ( n =4) were treated with IL-1β (1 ng ml −1 ), TNFα (10 ng ml −1 ) and TGFβ (10 ng ml −1 ) for 48 h, and miR-145 expression was then determined by real-time PCR. The data show the mean±s.d. * P
    Figure Legend Snippet: microRNA profiling in RA-FLSs. ( a ) Heatmap of microarray data showing DEmiRs among RA-FLSs versus OA-FLSs, RA-FLSs with IL-1β versus RA-FLSs, OA-FLSs with IL-1β versus OA-FLSs and RA-FLSs with IL-1β versus OA-FLSs with IL-1β. Red and green denote high and low expression of DEmiRs, respectively; red and green denote high and low expression of DEGs, respectively. ( b ) Higher levels of miR-143 and miR-145 in RA-FLSs ( n =5) than in OA-FLSs ( n =5) as determined by quantitative real-time PCR; 5.6-fold, P =0.031 for miR-143; 2.6-fold, P =0.028 for miR-145. RUN6B_2 small nuclear RNA (snRNA) was used as an internal control. ( c ) Spearman correlation of relative expression of miR-143 and miR-145 in RA-FLSs ( n =8). ( d ) miR-143 and miR-145 levels in synovial fluids obtained from RA patients (RA-SF, n =4) and OA patients (OA-SF, n =5) as determined by real-time PCR. ( e ) Increase in miR-145 expression in FLSs by TGFβ stimulation. The OA-FLSs ( n =4) and RA-FLSs ( n =4) were treated with IL-1β (1 ng ml −1 ), TNFα (10 ng ml −1 ) and TGFβ (10 ng ml −1 ) for 48 h, and miR-145 expression was then determined by real-time PCR. The data show the mean±s.d. * P

    Techniques Used: Microarray, Expressing, Real-time Polymerase Chain Reaction

    Hypothetical model for the role of miR-143 and miR-145 in RA-FLSs.
    Figure Legend Snippet: Hypothetical model for the role of miR-143 and miR-145 in RA-FLSs.

    Techniques Used:

    7) Product Images from "Isolation and Characterization of Two Novel Plasmids from Pathogenic Leptospira interrogans Serogroup Canicola Serovar Canicola Strain Gui44"

    Article Title: Isolation and Characterization of Two Novel Plasmids from Pathogenic Leptospira interrogans Serogroup Canicola Serovar Canicola Strain Gui44

    Journal: PLoS Neglected Tropical Diseases

    doi: 10.1371/journal.pntd.0003103

    Characterization of pGui1 and pGui2 in the pathogenic L.interrogans strain Gui44 by PFGE and Southern blot. PFGE and Southern Blot (using radiolabeled spGui1 probe) of undigested plasmid DNA (lanes A1 and B1); plasmid DNA digested with NotI (A2 and B2); plasmid DNA digested with BamHI (A3 and B3); total genomic DNA digested with NotI (A4 and B4); and undigested total genomic DNA (A5 and B5). The fragment in A2 and B2 indicates the linearized pGui1 (∼78 kb). The corresponding fragment presents in A4 and B4. The larger fragment in A3 indicates the linearized pGui2. The smaller fragment in A4 is one fragment of pGui1 digested with BamHI. Salmonella Braenderup (H9812) digested with XbaI and is used as the molecular weight size standard (M).
    Figure Legend Snippet: Characterization of pGui1 and pGui2 in the pathogenic L.interrogans strain Gui44 by PFGE and Southern blot. PFGE and Southern Blot (using radiolabeled spGui1 probe) of undigested plasmid DNA (lanes A1 and B1); plasmid DNA digested with NotI (A2 and B2); plasmid DNA digested with BamHI (A3 and B3); total genomic DNA digested with NotI (A4 and B4); and undigested total genomic DNA (A5 and B5). The fragment in A2 and B2 indicates the linearized pGui1 (∼78 kb). The corresponding fragment presents in A4 and B4. The larger fragment in A3 indicates the linearized pGui2. The smaller fragment in A4 is one fragment of pGui1 digested with BamHI. Salmonella Braenderup (H9812) digested with XbaI and is used as the molecular weight size standard (M).

    Techniques Used: Southern Blot, Plasmid Preparation, Molecular Weight

    8) Product Images from "Features of autophagic cell death in Plasmodium liver-stage parasites"

    Article Title: Features of autophagic cell death in Plasmodium liver-stage parasites

    Journal: Autophagy

    doi: 10.4161/auto.23689

    Figure 7. PbAtg8 does not localize to autophagosomes in dying parasites ( A ) and cannot complement yeast Atg8 ( B ). ( A ) HepG2 cells were infected with P. berghei parasites constitutively expressing mCherry (mCherry). 60 hpi the cells were fixed and stained with an anti-PbACP antiserum (ACP) to label the apicoplast and an anti-PbAtg8 antiserum (PbAtg8) to monitor the localization of this protein during parasite cell death. DNA was labeled with DAPI. A representative parasite showing strong vacuolization is depicted. A higher magnification of some important details is presented in the merged image. Scale bar: 10 µm: CPS. ( B ) Scatg8Δ and wt strains were transformed with empty pFL61 plasmid or the same plasmid containing PbAtg8 or ScAtg8 as a positive control. Western blot analysis was performed with the transformed strains using anti-aminopeptidase I antibodies. Transport of prApe1 to the vacuole where it matures (Ape1) only takes place in the presence of a functional autophagy pathway. The prApe1 and the Ape1 bands are marked with arrows.
    Figure Legend Snippet: Figure 7. PbAtg8 does not localize to autophagosomes in dying parasites ( A ) and cannot complement yeast Atg8 ( B ). ( A ) HepG2 cells were infected with P. berghei parasites constitutively expressing mCherry (mCherry). 60 hpi the cells were fixed and stained with an anti-PbACP antiserum (ACP) to label the apicoplast and an anti-PbAtg8 antiserum (PbAtg8) to monitor the localization of this protein during parasite cell death. DNA was labeled with DAPI. A representative parasite showing strong vacuolization is depicted. A higher magnification of some important details is presented in the merged image. Scale bar: 10 µm: CPS. ( B ) Scatg8Δ and wt strains were transformed with empty pFL61 plasmid or the same plasmid containing PbAtg8 or ScAtg8 as a positive control. Western blot analysis was performed with the transformed strains using anti-aminopeptidase I antibodies. Transport of prApe1 to the vacuole where it matures (Ape1) only takes place in the presence of a functional autophagy pathway. The prApe1 and the Ape1 bands are marked with arrows.

    Techniques Used: Infection, Expressing, Staining, Labeling, Transformation Assay, Plasmid Preparation, Positive Control, Western Blot, Functional Assay

    9) Product Images from "MicroRNA-143 and -145 modulate the phenotype of synovial fibroblasts in rheumatoid arthritis"

    Article Title: MicroRNA-143 and -145 modulate the phenotype of synovial fibroblasts in rheumatoid arthritis

    Journal: Experimental & Molecular Medicine

    doi: 10.1038/emm.2017.108

    Validation of IGFBP5 and SEMA3A as miR-143 and miR-145 targets, respectively. ( a ) Comparison of IGFBP5 mRNA expression between RA-FLSs ( n =7) and OA-FLSs ( n =10), which was determined by real-time PCR. GAPDH mRNA was used as an internal control. miR-143 HIGH ( n =3) and miR-143 LOW ( n =4) indicate RA-FLS subsets with higher and lower expression of miR-143, respectively. ( b ) Negative correlation between miR-143 and IGFBP5 expression in RA-FLSs ( n =7). ( c ) Negative correlation of IGFBP5 gene expression levels with eight TFGβ-responsive genes in RA-FLSs ( n =19) used in the previous study by Kasperkovitz et al. 19 (GSE4061) and those ( n =6) in this study (GSE22956 and GSE49604). ACTA2 , Actin, Alpha 2, Smooth Muscle, Aorta; COL3A1 , Collagen Type III Alpha 1 Chain; COL4A2 , Collagen Type IV Alpha 2 Chain; POSTN , Periostin; SPARC , secreted protein acidic and rich in cysteine; CYR61 , Cysteine Rich Angiogenic Inducer 61; SERPINH1 , Serine (or Cysteine) Proteinase Inhibitor, Clade H (Heat Shock Protein 47), Member 1 (Collagen Binding Protein 1); CALD1 , Caldesmon 1. Values are Pearson’s rho coefficients. ( d ) SEMA3A mRNA expression in RA-FLSs ( n =7) versus OA-FLSs ( n =10). miR-145 HIGH ( n =3) and miR-145 LOW ( n =4) indicate RA-FLS subsets with higher and lower expression of miR-145, respectively. ( e ) Sequence of miR-145 seed regions in the SEMA3A 3′-UTR. The mutation sequences are indicated in blue. ( f ) Relative normalized Renilla luciferase activity of reporter vector containing putative miR-145 binding sites (WT) or its mutated version (MUT). HEK293T cells were transfected with reporter vectors in the presence or absence of control mimic or synthetic miR-145 miRNA mimic. Single mutants of two putative miR-145-binding sites (seed regions) are indicated as WT-MUT or MUT-WT. A double mutant is denoted as MUT-MUT. RLU, relative light unit. The data show the mean±s.d. of three independent experiments. * P
    Figure Legend Snippet: Validation of IGFBP5 and SEMA3A as miR-143 and miR-145 targets, respectively. ( a ) Comparison of IGFBP5 mRNA expression between RA-FLSs ( n =7) and OA-FLSs ( n =10), which was determined by real-time PCR. GAPDH mRNA was used as an internal control. miR-143 HIGH ( n =3) and miR-143 LOW ( n =4) indicate RA-FLS subsets with higher and lower expression of miR-143, respectively. ( b ) Negative correlation between miR-143 and IGFBP5 expression in RA-FLSs ( n =7). ( c ) Negative correlation of IGFBP5 gene expression levels with eight TFGβ-responsive genes in RA-FLSs ( n =19) used in the previous study by Kasperkovitz et al. 19 (GSE4061) and those ( n =6) in this study (GSE22956 and GSE49604). ACTA2 , Actin, Alpha 2, Smooth Muscle, Aorta; COL3A1 , Collagen Type III Alpha 1 Chain; COL4A2 , Collagen Type IV Alpha 2 Chain; POSTN , Periostin; SPARC , secreted protein acidic and rich in cysteine; CYR61 , Cysteine Rich Angiogenic Inducer 61; SERPINH1 , Serine (or Cysteine) Proteinase Inhibitor, Clade H (Heat Shock Protein 47), Member 1 (Collagen Binding Protein 1); CALD1 , Caldesmon 1. Values are Pearson’s rho coefficients. ( d ) SEMA3A mRNA expression in RA-FLSs ( n =7) versus OA-FLSs ( n =10). miR-145 HIGH ( n =3) and miR-145 LOW ( n =4) indicate RA-FLS subsets with higher and lower expression of miR-145, respectively. ( e ) Sequence of miR-145 seed regions in the SEMA3A 3′-UTR. The mutation sequences are indicated in blue. ( f ) Relative normalized Renilla luciferase activity of reporter vector containing putative miR-145 binding sites (WT) or its mutated version (MUT). HEK293T cells were transfected with reporter vectors in the presence or absence of control mimic or synthetic miR-145 miRNA mimic. Single mutants of two putative miR-145-binding sites (seed regions) are indicated as WT-MUT or MUT-WT. A double mutant is denoted as MUT-MUT. RLU, relative light unit. The data show the mean±s.d. of three independent experiments. * P

    Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Binding Assay, Sequencing, Mutagenesis, Luciferase, Activity Assay, Plasmid Preparation, Transfection

    10) Product Images from "Association of iss and iucA, but Not tsh, with Plasmid-Mediated Virulence of Avian Pathogenic Escherichia coli"

    Article Title: Association of iss and iucA, but Not tsh, with Plasmid-Mediated Virulence of Avian Pathogenic Escherichia coli

    Journal:

    doi: 10.1128/IAI.72.11.6554-6560.2004

    Physical map of plasmid pVM01::Tn phoA illustrating the NotI, SpeI, and XbaI restriction endonuclease cleavage sites and the locations of the Tn phoA insert and the putative virulence genes, iucA , iss , and tsh. Designation of fragments (A,B,C, or D) corresponds
    Figure Legend Snippet: Physical map of plasmid pVM01::Tn phoA illustrating the NotI, SpeI, and XbaI restriction endonuclease cleavage sites and the locations of the Tn phoA insert and the putative virulence genes, iucA , iss , and tsh. Designation of fragments (A,B,C, or D) corresponds

    Techniques Used: Plasmid Preparation

    Related Articles

    Clone Assay:

    Article Title: Features of autophagic cell death in Plasmodium liver-stage parasites
    Article Snippet: .. The fragments were cloned into the yeast expression vector pFL61 via NotI (New England Biolabs, R0189) and the resulting plasmids used to transform Scatg8Δ WCG strains of S. cerevisiae by the acetate method. .. In addition, the Scatg8Δ WCG strain and the wild-type WCG strain were transformed with the empty vector.

    Positron Emission Tomography:

    Article Title: Comparing Galactan Biosynthesis in Mycobacterium tuberculosis and Corynebacterium diphtheriae *
    Article Snippet: .. Purified PCR product and pET-24a(+) vector (EMD Chemicals) were digested with NdeI and NotI restriction endonucleases (New England Biolabs). .. The double-digested products were purified using the QIAquick Gel Extraction kit (Qiagen).

    Expressing:

    Article Title: Features of autophagic cell death in Plasmodium liver-stage parasites
    Article Snippet: .. The fragments were cloned into the yeast expression vector pFL61 via NotI (New England Biolabs, R0189) and the resulting plasmids used to transform Scatg8Δ WCG strains of S. cerevisiae by the acetate method. .. In addition, the Scatg8Δ WCG strain and the wild-type WCG strain were transformed with the empty vector.

    Purification:

    Article Title: Comparing Galactan Biosynthesis in Mycobacterium tuberculosis and Corynebacterium diphtheriae *
    Article Snippet: .. Purified PCR product and pET-24a(+) vector (EMD Chemicals) were digested with NdeI and NotI restriction endonucleases (New England Biolabs). .. The double-digested products were purified using the QIAquick Gel Extraction kit (Qiagen).

    Article Title: Positional Cloning of the Mouse Circadian Clock Gene
    Article Snippet: .. After NotI digestion and separation by FIGE, BAC insert DNA was purified by i -agarase digestion (New England Biolabs). .. Radiolabeled probe was generated using the DECAprime II kit (Ambion) with gel-purified, full-length BAC insert as template; probe was purified with a ProbeQuant G-50 Micro column (Pharmacia).

    Article Title: Calbindin-D32k Is Localized to a Subpopulation of Neurons in the Nervous System of the Sea Cucumber Holothuria glaberrima (Echinodermata)
    Article Snippet: .. This product was purified using a QIAGEN Spin Column (QIAGEN, Valencia, CA) and inserted into pGEX6P-1 (GE Healthcare Biosciences, Piscataway, NJ), using BamH1 and NotI restriction enzymes (New England Biolabs, Ipswich, MA). .. The vector was previously treated with Calf Intestinal Alkaline Phosphatase (Invitrogen, Carlsbad, CA) diluted to 0.01 u/uL in Buffer CIAP.

    Plasmid Preparation:

    Article Title: Comparing Galactan Biosynthesis in Mycobacterium tuberculosis and Corynebacterium diphtheriae *
    Article Snippet: .. Purified PCR product and pET-24a(+) vector (EMD Chemicals) were digested with NdeI and NotI restriction endonucleases (New England Biolabs). .. The double-digested products were purified using the QIAquick Gel Extraction kit (Qiagen).

    Article Title: Features of autophagic cell death in Plasmodium liver-stage parasites
    Article Snippet: .. The fragments were cloned into the yeast expression vector pFL61 via NotI (New England Biolabs, R0189) and the resulting plasmids used to transform Scatg8Δ WCG strains of S. cerevisiae by the acetate method. .. In addition, the Scatg8Δ WCG strain and the wild-type WCG strain were transformed with the empty vector.

    BAC Assay:

    Article Title: Positional Cloning of the Mouse Circadian Clock Gene
    Article Snippet: .. After NotI digestion and separation by FIGE, BAC insert DNA was purified by i -agarase digestion (New England Biolabs). .. Radiolabeled probe was generated using the DECAprime II kit (Ambion) with gel-purified, full-length BAC insert as template; probe was purified with a ProbeQuant G-50 Micro column (Pharmacia).

    Polymerase Chain Reaction:

    Article Title: Comparing Galactan Biosynthesis in Mycobacterium tuberculosis and Corynebacterium diphtheriae *
    Article Snippet: .. Purified PCR product and pET-24a(+) vector (EMD Chemicals) were digested with NdeI and NotI restriction endonucleases (New England Biolabs). .. The double-digested products were purified using the QIAquick Gel Extraction kit (Qiagen).

    Pulsed-Field Gel:

    Article Title: Transmission of Yersinia pseudotuberculosis in the Pork Production Chain from Farm to Slaughterhouse ▿
    Article Snippet: .. A total of 286 isolates were characterized by pulsed-field gel electrophoresis (PFGE) as described by Niskanen et al. ( ) using SpeI and NotI restriction enzymes (New England Biolabs, Ipswich, MA). ..

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    New England Biolabs noti restriction enzymes
    Digestion of pCMV-RAE-1γ with <t>KpnI</t> and <t>NotI.</t>
    Noti Restriction Enzymes, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/noti restriction enzymes/product/New England Biolabs
    Average 97 stars, based on 23 article reviews
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    noti restriction enzymes - by Bioz Stars, 2020-05
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    Digestion of pCMV-RAE-1γ with KpnI and NotI.

    Journal: Journal of Controlled Release

    Article Title: EphA2 targeting peptide tethered bioreducible poly(cystamine bisacrylamide - diamino hexane) for the delivery of therapeutic pCMV-RAE-1? to pancreatic islets

    doi: 10.1016/j.jconrel.2011.10.022

    Figure Lengend Snippet: Digestion of pCMV-RAE-1γ with KpnI and NotI.

    Article Snippet: KpnI and NotI restriction enzymes were obtained from New England Biolabs (Ipswich, MA).

    Techniques:

    Genetic, Physical, and Transcription Unit Mapping of the Clock Locus (A) Genetic map of mouse chromosome 5, showing the location of Clock on the midportion of this chromosome, 0.7 cM distal of Kit , flanked by D5Mit307 and D5Mit112 . (B) Physical and high resolution genetic mapping of the Clock locus. The SSLPs and STSs in the D5Mit307 –D5Mit112 interval are shown on the upper bar. The maximum nonrecombinant interval is defined by the SSLPs D5Mit307 and D5Nwu2 . The animals with recombinations between these SSLPs and Clock are noted. Also shown are restriction sites for NotI (depicted with an [N]), which identify two CpG islands associated with the 5′ ends of Clock and pFT27 . YAC and BAC contigs of the Clock . The BAC clones were isolated from a library constructed in Dr. Melvin Simon’s laboratory. (C) Candidate genes mapping to the nonrecombinant interval. Shown are their relative locations and transcriptional orientations.

    Journal: Cell

    Article Title: Positional Cloning of the Mouse Circadian Clock Gene

    doi:

    Figure Lengend Snippet: Genetic, Physical, and Transcription Unit Mapping of the Clock Locus (A) Genetic map of mouse chromosome 5, showing the location of Clock on the midportion of this chromosome, 0.7 cM distal of Kit , flanked by D5Mit307 and D5Mit112 . (B) Physical and high resolution genetic mapping of the Clock locus. The SSLPs and STSs in the D5Mit307 –D5Mit112 interval are shown on the upper bar. The maximum nonrecombinant interval is defined by the SSLPs D5Mit307 and D5Nwu2 . The animals with recombinations between these SSLPs and Clock are noted. Also shown are restriction sites for NotI (depicted with an [N]), which identify two CpG islands associated with the 5′ ends of Clock and pFT27 . YAC and BAC contigs of the Clock . The BAC clones were isolated from a library constructed in Dr. Melvin Simon’s laboratory. (C) Candidate genes mapping to the nonrecombinant interval. Shown are their relative locations and transcriptional orientations.

    Article Snippet: After NotI digestion and separation by FIGE, BAC insert DNA was purified by i -agarase digestion (New England Biolabs).

    Techniques: BAC Assay, Clone Assay, Isolation, Construct

    Four different NotI profiles (lanes 1 to 4) of Yersinia pseudotuberculosis strains obtained. M designates midrange PFGE markers.

    Journal: Applied and Environmental Microbiology

    Article Title: Transmission of Yersinia pseudotuberculosis in the Pork Production Chain from Farm to Slaughterhouse ▿

    doi: 10.1128/AEM.02664-07

    Figure Lengend Snippet: Four different NotI profiles (lanes 1 to 4) of Yersinia pseudotuberculosis strains obtained. M designates midrange PFGE markers.

    Article Snippet: A total of 286 isolates were characterized by pulsed-field gel electrophoresis (PFGE) as described by Niskanen et al. ( ) using SpeI and NotI restriction enzymes (New England Biolabs, Ipswich, MA).

    Techniques:

    Characterization of pGui1 and pGui2 in the pathogenic L.interrogans strain Gui44 by PFGE and Southern blot. PFGE and Southern Blot (using radiolabeled spGui1 probe) of undigested plasmid DNA (lanes A1 and B1); plasmid DNA digested with NotI (A2 and B2); plasmid DNA digested with BamHI (A3 and B3); total genomic DNA digested with NotI (A4 and B4); and undigested total genomic DNA (A5 and B5). The fragment in A2 and B2 indicates the linearized pGui1 (∼78 kb). The corresponding fragment presents in A4 and B4. The larger fragment in A3 indicates the linearized pGui2. The smaller fragment in A4 is one fragment of pGui1 digested with BamHI. Salmonella Braenderup (H9812) digested with XbaI and is used as the molecular weight size standard (M).

    Journal: PLoS Neglected Tropical Diseases

    Article Title: Isolation and Characterization of Two Novel Plasmids from Pathogenic Leptospira interrogans Serogroup Canicola Serovar Canicola Strain Gui44

    doi: 10.1371/journal.pntd.0003103

    Figure Lengend Snippet: Characterization of pGui1 and pGui2 in the pathogenic L.interrogans strain Gui44 by PFGE and Southern blot. PFGE and Southern Blot (using radiolabeled spGui1 probe) of undigested plasmid DNA (lanes A1 and B1); plasmid DNA digested with NotI (A2 and B2); plasmid DNA digested with BamHI (A3 and B3); total genomic DNA digested with NotI (A4 and B4); and undigested total genomic DNA (A5 and B5). The fragment in A2 and B2 indicates the linearized pGui1 (∼78 kb). The corresponding fragment presents in A4 and B4. The larger fragment in A3 indicates the linearized pGui2. The smaller fragment in A4 is one fragment of pGui1 digested with BamHI. Salmonella Braenderup (H9812) digested with XbaI and is used as the molecular weight size standard (M).

    Article Snippet: The plugs were then washed twice with pre-warmed TE buffer, and then digested with either restriction enzyme NotI or BamHI at 37°C or 30°C, respectively, following manufacturer's instructions (New England Biolabs, Ipswich, MA, USA).

    Techniques: Southern Blot, Plasmid Preparation, Molecular Weight