fnu4hi  (New England Biolabs)


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    Name:
    Fnu4HI
    Description:
    Fnu4HI 1 000 units
    Catalog Number:
    R0178L
    Price:
    282
    Category:
    Restriction Enzymes
    Size:
    1 000 units
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    Structured Review

    New England Biolabs fnu4hi
    Fnu4HI
    Fnu4HI 1 000 units
    https://www.bioz.com/result/fnu4hi/product/New England Biolabs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fnu4hi - by Bioz Stars, 2021-06
    92/100 stars

    Images

    1) Product Images from "Quantitative detection of DNMT3A R882H mutation in acute myeloid leukemia"

    Article Title: Quantitative detection of DNMT3A R882H mutation in acute myeloid leukemia

    Journal: Journal of Experimental & Clinical Cancer Research : CR

    doi: 10.1186/s13046-015-0173-2

    Concordance of ASB-PCR. a Representative endonuclease restriction analysis of follow-up samples of five patients (A-E). Wild type samples show two bands at 190 bp and 114 bp. Positive samples display three bands at 289 bp, 190 bp, 114 bp due to the loss of a restriction site of Fnu4HI caused by the mutation. Hyperladder II (Bioline) was used as marker. b Results of ASB-PCR analysis
    Figure Legend Snippet: Concordance of ASB-PCR. a Representative endonuclease restriction analysis of follow-up samples of five patients (A-E). Wild type samples show two bands at 190 bp and 114 bp. Positive samples display three bands at 289 bp, 190 bp, 114 bp due to the loss of a restriction site of Fnu4HI caused by the mutation. Hyperladder II (Bioline) was used as marker. b Results of ASB-PCR analysis

    Techniques Used: Polymerase Chain Reaction, Mutagenesis, Marker

    2) Product Images from "CTLA-4 gene polymorphisms and their influence on predisposition to autoimmune thyroid diseases (Graves' disease and Hashimoto's thyroiditis)"

    Article Title: CTLA-4 gene polymorphisms and their influence on predisposition to autoimmune thyroid diseases (Graves' disease and Hashimoto's thyroiditis)

    Journal: Archives of Medical Science : AMS

    doi: 10.5114/aoms.2012.28593

    Electropherogram of PCR-RFLP products of CTLA-4 A49G genotypes; Fnu4HI digestion (A/G polymorphism) Lines 1, 3 – heterozygous A/G patients, with GD and HT, respectively, lines 2, 4, 5 – homozygous A/A patients with GD, M – DNA pUC19/MspI length marker
    Figure Legend Snippet: Electropherogram of PCR-RFLP products of CTLA-4 A49G genotypes; Fnu4HI digestion (A/G polymorphism) Lines 1, 3 – heterozygous A/G patients, with GD and HT, respectively, lines 2, 4, 5 – homozygous A/A patients with GD, M – DNA pUC19/MspI length marker

    Techniques Used: Polymerase Chain Reaction, Marker

    3) Product Images from "Comparative examination of various PCR-based methods for DNMT3A and IDH1/2 mutations identification in acute myeloid leukemia"

    Article Title: Comparative examination of various PCR-based methods for DNMT3A and IDH1/2 mutations identification in acute myeloid leukemia

    Journal: Journal of Experimental & Clinical Cancer Research : CR

    doi: 10.1186/1756-9966-33-44

    Restriction analysis of DNMT3A R882H mutations. 1) Agarose gel analysis of restricted products of 5 positive (12, 34, 57, 65, 187) and 2 negative (54, 143) patients. Wt samples showed 2 bands at 190 bp and 114 bp. Positive samples showed 3 bands at 289 bp, 190 bp, 114 bp because of the loss of a restriction site of Fnu4HI caused by the mutation. Hyperladder II (Bioline) was used as the marker. 2) Representative sequence analysis of patient 187 showing heterozygote mutation CGC to CAC.
    Figure Legend Snippet: Restriction analysis of DNMT3A R882H mutations. 1) Agarose gel analysis of restricted products of 5 positive (12, 34, 57, 65, 187) and 2 negative (54, 143) patients. Wt samples showed 2 bands at 190 bp and 114 bp. Positive samples showed 3 bands at 289 bp, 190 bp, 114 bp because of the loss of a restriction site of Fnu4HI caused by the mutation. Hyperladder II (Bioline) was used as the marker. 2) Representative sequence analysis of patient 187 showing heterozygote mutation CGC to CAC.

    Techniques Used: Agarose Gel Electrophoresis, Mutagenesis, Marker, Sequencing

    4) Product Images from "Defining characteristics of Tn5 Transposase non-specific DNA binding"

    Article Title: Defining characteristics of Tn5 Transposase non-specific DNA binding

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gkl179

    Tn 5 Tnp interacts with supercoiled pUC19. REBA of Tnp bound to supercoiled pUC19 is shown in lanes 4–9 and DNA size markers are shown in lanes 1–3. Increasing concentrations of Tnp (lanes 5–9) were incubated with pUC19 followed by addition of frequent cutting restriction enzymes, HhaI, Fnu4HI and Sau3AI. Blockage of restriction sites by Tnp results in the appearance of larger bands and decrease in some smaller bands as Tnp concentration is increased. This is most evident when examining the overlay of the traces from a no Tnp control reaction (blue, lane 4) and 900 µM Tnp lanes (red, lane 9), seen to the right of the gel. Bands increasing in intensity are marked with a plus sign (+). Bands decreasing in intensity are marked with a minus sign (−).
    Figure Legend Snippet: Tn 5 Tnp interacts with supercoiled pUC19. REBA of Tnp bound to supercoiled pUC19 is shown in lanes 4–9 and DNA size markers are shown in lanes 1–3. Increasing concentrations of Tnp (lanes 5–9) were incubated with pUC19 followed by addition of frequent cutting restriction enzymes, HhaI, Fnu4HI and Sau3AI. Blockage of restriction sites by Tnp results in the appearance of larger bands and decrease in some smaller bands as Tnp concentration is increased. This is most evident when examining the overlay of the traces from a no Tnp control reaction (blue, lane 4) and 900 µM Tnp lanes (red, lane 9), seen to the right of the gel. Bands increasing in intensity are marked with a plus sign (+). Bands decreasing in intensity are marked with a minus sign (−).

    Techniques Used: Incubation, Concentration Assay

    Related Articles

    Polymerase Chain Reaction:

    Article Title: CTLA-4 gene polymorphisms and their influence on predisposition to autoimmune thyroid diseases (Graves' disease and Hashimoto's thyroiditis)
    Article Snippet: Quality and quantity of each DNA sample were assessed spectrophotometrically (NanoDrop Spectrophotometer ND-1000, Thermo-Scientific, USA). .. Analysis of CTLA-4 polymorphism CTLA-4 polymorphisms (A49G, 1822 C/T and CT60 A/G) were assessed by PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism), using the following restriction enzymes: Fnu4HI , BsmAI , BsaAI (New England Biolabs, USA). .. First, genomic DNA sequences containing the polymorphic region, i.e., Fnu4HI or BsmAI or BsaAI restriction site, were amplified in a PCR reaction (Personal Thermocycler, Eppendorf, Germany), in a total volume of 25 µl, including: 3 µl 10x AmpliTaq Gold® 360 buffer (150 mM Tris-HCl, pH 8.3, 500 mM KCl), 0.12 µl (5 U/ µl) AmpliTaq Gold® 360 DNA Polymerase, 2 µl (25 mM) MgCl2 , 0.66 µl (10 mM) dNTPs (Applied Biosystems, USA), 1 µl (40 ng) DNA, 2.4 µl (1.2 µl 0.5 µM each primer: forward and reverse) and 15.82 µl nuclease-free water.

    Article Title: Comparative examination of various PCR-based methods for DNMT3A and IDH1/2 mutations identification in acute myeloid leukemia
    Article Snippet: PCR reaction mixture was prepared as that described for ARMS assay. .. In all, 10 μl of the PCR product was directly applied for endonuclease treatment with 1 μl Fnu4HI and 5 μl of CutSmart Buffer (New England Biolabs). .. After incubation at 37°C for 15 min products were analysed on a 1.5% agarose gel containing 10% ethidium bromide (voltage 150 V).

    Generated:

    Article Title: Maternal cell traffic bounds for immune modulation: tracking maternal H-2 alleles in spleens of baby mice by DNA fingerprinting
    Article Snippet: This was because the H-2d allele is considerably larger than the rest of the H-2 haplotypes and can easily be resolved by 8% PAGE (lanes 1–5). .. The RFLP analysis generated by Fnu 4H I or Bbr I, however, was able to discriminate between the various alleles. ..

    Mouse Assay:

    Article Title: Maternal cell traffic bounds for immune modulation: tracking maternal H-2 alleles in spleens of baby mice by DNA fingerprinting
    Article Snippet: PCR products of the maternal immunized T-cell donor mice [BALB/c (H-2d )], the ‘d’ bands in the F1 mother mice (H-2d/b ) who had accepted immunized T cells (H-2d ) and in their F2 baby mice (H-2d/b ) were also purified and sequenced. .. shows the alignment of DNA sequences from a portion of the class II Eb gene in three different H-2 haplotype mice , and the predicted fragment sizes after Fnu 4H I (or Bbr I) treatment of those DNA sequences ( ). .. The three haplotypes represent two sequence lengths owing to the variable number of tetranucleotide repeats.

    Mutagenesis:

    Article Title: Quantitative detection of DNMT3A R882H mutation in acute myeloid leukemia
    Article Snippet: Sequencing was performed using ABI310 Genetic Analyzer (Applied Biosystems), and data were analyzed using DNA Sequencing Analysis Software v.5.2.0. .. Endonuclease restriction analysis of DNMT3A R882H mutation was performed using Fnu4HI (New England Biolabs) as previously reported [ ]. .. Qualitative and quantitative evaluation of mutations Qualitative evaluation for presence of NPM1 , DNMT3A , IDH1 and IDH2 mutations were performed by Sanger sequencing using ABI310 Genetic Analyzer (Applied Biosystems) as previously described [ ].

    Incubation:

    Article Title: Defining characteristics of Tn5 Transposase non-specific DNA binding
    Article Snippet: .. Following a 1 hour incubation, 20 U of HhaI (NEB), 20 units of Fnu4HI (NEB) and 16 U of Sau3AI (NEB) were added to each reaction and incubation at 37°C continued for an additional three hours. .. Each reaction was then phenol/chloroform extracted twice and concentrated by ethanol precipitation with glycogen.

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    New England Biolabs fnu 4h i
    The H-2 d/b allele of the engrafted T cells can be found in the spleens of the F2 H-2 d/d baby mice. Lanes 1–5 show the bands before treatment with <t>Fnu</t> <t>4H</t> I and lanes 6–10, the bands after treatment with Fnu 4H I. Lanes 1 and 6 show the normal mouse (H-2 d , tail), lanes 2 and 7 the normal mouse (H-2 d/b , tail), lanes 3 and 8 the T-cell donor mouse (H-2 d/b tail), lanes 4 and 9 the F1 normal mother mouse accepted immunized T cells (H-2 d/b , tail), and lanes 5 and 10 the F2 baby mouse (H-2 d/d , spleen) alleles. M, puc19/ Msp I size markers. Note, in lane 5, the mother mouse's allele (H-2 d/b ) can be resolved, and in lane 10, the H-2 d/b restriction patterns (208-bp fragment and 85-bp fragment) can also be found.
    Fnu 4h I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fnu 4h i/product/New England Biolabs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fnu 4h i - by Bioz Stars, 2021-06
    92/100 stars
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    The H-2 d/b allele of the engrafted T cells can be found in the spleens of the F2 H-2 d/d baby mice. Lanes 1–5 show the bands before treatment with Fnu 4H I and lanes 6–10, the bands after treatment with Fnu 4H I. Lanes 1 and 6 show the normal mouse (H-2 d , tail), lanes 2 and 7 the normal mouse (H-2 d/b , tail), lanes 3 and 8 the T-cell donor mouse (H-2 d/b tail), lanes 4 and 9 the F1 normal mother mouse accepted immunized T cells (H-2 d/b , tail), and lanes 5 and 10 the F2 baby mouse (H-2 d/d , spleen) alleles. M, puc19/ Msp I size markers. Note, in lane 5, the mother mouse's allele (H-2 d/b ) can be resolved, and in lane 10, the H-2 d/b restriction patterns (208-bp fragment and 85-bp fragment) can also be found.

    Journal: Immunology

    Article Title: Maternal cell traffic bounds for immune modulation: tracking maternal H-2 alleles in spleens of baby mice by DNA fingerprinting

    doi: 10.1046/j.1365-2567.2002.01499.x

    Figure Lengend Snippet: The H-2 d/b allele of the engrafted T cells can be found in the spleens of the F2 H-2 d/d baby mice. Lanes 1–5 show the bands before treatment with Fnu 4H I and lanes 6–10, the bands after treatment with Fnu 4H I. Lanes 1 and 6 show the normal mouse (H-2 d , tail), lanes 2 and 7 the normal mouse (H-2 d/b , tail), lanes 3 and 8 the T-cell donor mouse (H-2 d/b tail), lanes 4 and 9 the F1 normal mother mouse accepted immunized T cells (H-2 d/b , tail), and lanes 5 and 10 the F2 baby mouse (H-2 d/d , spleen) alleles. M, puc19/ Msp I size markers. Note, in lane 5, the mother mouse's allele (H-2 d/b ) can be resolved, and in lane 10, the H-2 d/b restriction patterns (208-bp fragment and 85-bp fragment) can also be found.

    Article Snippet: After resuspension in water, the DNA was treated with Fnu 4H I (New England Biolabs, Beverly, MA), according to the manufacturer's instructions.

    Techniques: Mouse Assay

    The restriction pattern of the H-2 d/b mother can be found in the spleens of her F2 H-2 d/d baby mice. Lanes 1–4 show the bands before treatment with Fnu 4H I and lanes 5–8, the bands after treatment with Fnu 4H I. Lanes 1 and 5 show the normal mouse (H-2 d , tail), lanes 2 and 6 the normal mouse (H-2 d/b , tail), lanes 3 and 7 the immunized mother mouse (H-2 d/b tail), and lanes 4 and 8 the F2 baby mouse (H-2 d/d , spleen) alleles. M, pue19/ Msp I size markers (in bp): 501, 489, 404, 331, 242, 190, 147, 111/110 and 67, from top to bottom, respectively. Note, on lane 4, the allele (H-2 d/b ) of the mother mouse can be resolved, and on lane 8, the H-2 d/b restriction patterns (208-bp fragment and 85-bp fragment) can also be found.

    Journal: Immunology

    Article Title: Maternal cell traffic bounds for immune modulation: tracking maternal H-2 alleles in spleens of baby mice by DNA fingerprinting

    doi: 10.1046/j.1365-2567.2002.01499.x

    Figure Lengend Snippet: The restriction pattern of the H-2 d/b mother can be found in the spleens of her F2 H-2 d/d baby mice. Lanes 1–4 show the bands before treatment with Fnu 4H I and lanes 5–8, the bands after treatment with Fnu 4H I. Lanes 1 and 5 show the normal mouse (H-2 d , tail), lanes 2 and 6 the normal mouse (H-2 d/b , tail), lanes 3 and 7 the immunized mother mouse (H-2 d/b tail), and lanes 4 and 8 the F2 baby mouse (H-2 d/d , spleen) alleles. M, pue19/ Msp I size markers (in bp): 501, 489, 404, 331, 242, 190, 147, 111/110 and 67, from top to bottom, respectively. Note, on lane 4, the allele (H-2 d/b ) of the mother mouse can be resolved, and on lane 8, the H-2 d/b restriction patterns (208-bp fragment and 85-bp fragment) can also be found.

    Article Snippet: After resuspension in water, the DNA was treated with Fnu 4H I (New England Biolabs, Beverly, MA), according to the manufacturer's instructions.

    Techniques: Mouse Assay

    Polymerase chain reaction (PCR) and Fnu 4H I restriction patterns of three inbred and two hybrid (F1) mice strains. Identification of H-2 haplotypes by PCR amplification and restriction fragment length polymorphism (RFLP) is shown above (8% PAGE). Lanes 1–5 show the bands before treatment with Fnu 4H I and lanes 6–10, the bands after treatment with Fnu 4H I. Lanes 1 and 6 show the H-2 b/b mouse, lanes 2 and 7 the H-2 k/k mouse, lanes 3 and 8 the H-2 d/d mouse, lanes 4 and 9 the H-2 k/b mouse, and lanes 5 and 10 the H-2 d/b mouse. M, 100-bp DNA ladder size markers (in bp): 2072, 1500, 700, 600, 500, 400, 300, 200, 100, from top to bottom, respectively.

    Journal: Immunology

    Article Title: Maternal cell traffic bounds for immune modulation: tracking maternal H-2 alleles in spleens of baby mice by DNA fingerprinting

    doi: 10.1046/j.1365-2567.2002.01499.x

    Figure Lengend Snippet: Polymerase chain reaction (PCR) and Fnu 4H I restriction patterns of three inbred and two hybrid (F1) mice strains. Identification of H-2 haplotypes by PCR amplification and restriction fragment length polymorphism (RFLP) is shown above (8% PAGE). Lanes 1–5 show the bands before treatment with Fnu 4H I and lanes 6–10, the bands after treatment with Fnu 4H I. Lanes 1 and 6 show the H-2 b/b mouse, lanes 2 and 7 the H-2 k/k mouse, lanes 3 and 8 the H-2 d/d mouse, lanes 4 and 9 the H-2 k/b mouse, and lanes 5 and 10 the H-2 d/b mouse. M, 100-bp DNA ladder size markers (in bp): 2072, 1500, 700, 600, 500, 400, 300, 200, 100, from top to bottom, respectively.

    Article Snippet: After resuspension in water, the DNA was treated with Fnu 4H I (New England Biolabs, Beverly, MA), according to the manufacturer's instructions.

    Techniques: Polymerase Chain Reaction, Mouse Assay, Amplification, Polyacrylamide Gel Electrophoresis

    The portion of the Eb gene DNA sequence of three H-2 haplotypes. (a) Alignment of DNA sequences from three different H-2 haplotypes: H-2 d , H-2 k and H-2 b . The sequence contains the 3′ portion of intron 2 and the 5′ portion of exon β2 of the Eb gene. A dot ‘.’ indicates identity with and a hyphen ‘–’ implies absence of the nucleotide in the top line. The primer sequences at the two termini and the two internal Fnu 4H I sites are underlined. The sequences are from Kobori et al The β2 exon starts at nt 185. The microsatellite length polymorphism can be visualized by the hyphens (–). (b) Predicted fragment sizes after Fnu 4H I treatment of the DNA sequences shown above.

    Journal: Immunology

    Article Title: Maternal cell traffic bounds for immune modulation: tracking maternal H-2 alleles in spleens of baby mice by DNA fingerprinting

    doi: 10.1046/j.1365-2567.2002.01499.x

    Figure Lengend Snippet: The portion of the Eb gene DNA sequence of three H-2 haplotypes. (a) Alignment of DNA sequences from three different H-2 haplotypes: H-2 d , H-2 k and H-2 b . The sequence contains the 3′ portion of intron 2 and the 5′ portion of exon β2 of the Eb gene. A dot ‘.’ indicates identity with and a hyphen ‘–’ implies absence of the nucleotide in the top line. The primer sequences at the two termini and the two internal Fnu 4H I sites are underlined. The sequences are from Kobori et al The β2 exon starts at nt 185. The microsatellite length polymorphism can be visualized by the hyphens (–). (b) Predicted fragment sizes after Fnu 4H I treatment of the DNA sequences shown above.

    Article Snippet: After resuspension in water, the DNA was treated with Fnu 4H I (New England Biolabs, Beverly, MA), according to the manufacturer's instructions.

    Techniques: Sequencing

    Restriction of plasmid pKH11 prior to (odd lanes) and following (even lanes) methylation of 5′-GpC-3′ sites by the DNA 5-cytosine methyl-transferase M.Cvi PI (Materials and Methods). pKH11 was digested with Fnu 4HI, Bbv I, Eco P15I, Mwo I,

    Journal: Epigenetics

    Article Title: Identification of restriction endonucleases sensitive to 5-cytosine methylation at non-CpG sites, including expanded (CAG)n/(CTG)n repeats

    doi: 10.4161/epi.6.4.14953

    Figure Lengend Snippet: Restriction of plasmid pKH11 prior to (odd lanes) and following (even lanes) methylation of 5′-GpC-3′ sites by the DNA 5-cytosine methyl-transferase M.Cvi PI (Materials and Methods). pKH11 was digested with Fnu 4HI, Bbv I, Eco P15I, Mwo I,

    Article Snippet: Briefly, Ape KI digests were in NEBuffer #3 at 75°C, Bbv I digests were in NEBuffer #2 at 37°C, Eco P15I digests were in NEBuffer #3 at 37°C, Fnu 4HI digests were in NEBuffer #4 at 37°C, Mwo I digests were in NEBuffer #3 at 60°C and TseI digests were in NEBuffer #4 at 65°C.

    Techniques: Plasmid Preparation, Methylation, Gel Permeation Chromatography

    Concordance of ASB-PCR. a Representative endonuclease restriction analysis of follow-up samples of five patients (A-E). Wild type samples show two bands at 190 bp and 114 bp. Positive samples display three bands at 289 bp, 190 bp, 114 bp due to the loss of a restriction site of Fnu4HI caused by the mutation. Hyperladder II (Bioline) was used as marker. b Results of ASB-PCR analysis

    Journal: Journal of Experimental & Clinical Cancer Research : CR

    Article Title: Quantitative detection of DNMT3A R882H mutation in acute myeloid leukemia

    doi: 10.1186/s13046-015-0173-2

    Figure Lengend Snippet: Concordance of ASB-PCR. a Representative endonuclease restriction analysis of follow-up samples of five patients (A-E). Wild type samples show two bands at 190 bp and 114 bp. Positive samples display three bands at 289 bp, 190 bp, 114 bp due to the loss of a restriction site of Fnu4HI caused by the mutation. Hyperladder II (Bioline) was used as marker. b Results of ASB-PCR analysis

    Article Snippet: Endonuclease restriction analysis of DNMT3A R882H mutation was performed using Fnu4HI (New England Biolabs) as previously reported [ ].

    Techniques: Polymerase Chain Reaction, Mutagenesis, Marker