paer7i restriction enzymes  (New England Biolabs)


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    Name:
    PaeR7I
    Description:
    PaeR7I 2 000 units
    Catalog Number:
    r0177s
    Price:
    61
    Size:
    2 000 units
    Category:
    Restriction Enzymes
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    New England Biolabs paer7i restriction enzymes
    PaeR7I
    PaeR7I 2 000 units
    https://www.bioz.com/result/paer7i restriction enzymes/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    paer7i restriction enzymes - by Bioz Stars, 2020-03
    94/100 stars

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    Related Articles

    DNA Extraction:

    Article Title: Interleukin-27 as a Novel Biomarker for Early Cardiopulmonary Failure in Enterovirus 71-Infected Children with Central Nervous System Involvement
    Article Snippet: Paragraph title: 2.4. DNA Extraction and SNP Genotyping ... The primer sequences for the rs153109 SNP were F: 5′-TCAGTCAGTGACCAGGATCG-3′ and R: 5′-ACCAAGAAACCCCATCCTCT-3′, the annealing temperature was 58°C, and the PCR products were incubated with PaeR7I restriction enzymes (New England Biolabs, Ipswich, MA, USA) for 4 h. The primer sequences for rs17855750 were F: 5′-ATCTCGCCAGGAAGCTGCGC-3′ and R: 5′-CTGTTAGTGGGGGCCAGAAGGGA-3′, the annealing temperature was 62°C, and the PCR products were incubated with restriction enzymes BstUI restriction enzymes (New England Biolabs) for 8 h. The primer sequences for rs181206 were F: 5′-GCTTCAGCCCTTCCATGCCC-3′ and R: 5′-TCTACCTGGAAGCGGAGGTGCC-3′, the annealing temperature was 64°C, and the PCR products were incubated with FauI restriction enzymes (New England Biolabs) for 12 h. The reactions were performed at 94°C for 1.5 min followed by 34 cycles of 30 s at 94°C, 30 s at the annealing temperature indicated above, 30 s at 72°C, and finally one cycle of 5 min at 72°C.

    Agarose Gel Electrophoresis:

    Article Title: Interleukin-27 as a Novel Biomarker for Early Cardiopulmonary Failure in Enterovirus 71-Infected Children with Central Nervous System Involvement
    Article Snippet: The primer sequences for the rs153109 SNP were F: 5′-TCAGTCAGTGACCAGGATCG-3′ and R: 5′-ACCAAGAAACCCCATCCTCT-3′, the annealing temperature was 58°C, and the PCR products were incubated with PaeR7I restriction enzymes (New England Biolabs, Ipswich, MA, USA) for 4 h. The primer sequences for rs17855750 were F: 5′-ATCTCGCCAGGAAGCTGCGC-3′ and R: 5′-CTGTTAGTGGGGGCCAGAAGGGA-3′, the annealing temperature was 62°C, and the PCR products were incubated with restriction enzymes BstUI restriction enzymes (New England Biolabs) for 8 h. The primer sequences for rs181206 were F: 5′-GCTTCAGCCCTTCCATGCCC-3′ and R: 5′-TCTACCTGGAAGCGGAGGTGCC-3′, the annealing temperature was 64°C, and the PCR products were incubated with FauI restriction enzymes (New England Biolabs) for 12 h. The reactions were performed at 94°C for 1.5 min followed by 34 cycles of 30 s at 94°C, 30 s at the annealing temperature indicated above, 30 s at 72°C, and finally one cycle of 5 min at 72°C. .. The primer sequences for the rs153109 SNP were F: 5′-TCAGTCAGTGACCAGGATCG-3′ and R: 5′-ACCAAGAAACCCCATCCTCT-3′, the annealing temperature was 58°C, and the PCR products were incubated with PaeR7I restriction enzymes (New England Biolabs, Ipswich, MA, USA) for 4 h. The primer sequences for rs17855750 were F: 5′-ATCTCGCCAGGAAGCTGCGC-3′ and R: 5′-CTGTTAGTGGGGGCCAGAAGGGA-3′, the annealing temperature was 62°C, and the PCR products were incubated with restriction enzymes BstUI restriction enzymes (New England Biolabs) for 8 h. The primer sequences for rs181206 were F: 5′-GCTTCAGCCCTTCCATGCCC-3′ and R: 5′-TCTACCTGGAAGCGGAGGTGCC-3′, the annealing temperature was 64°C, and the PCR products were incubated with FauI restriction enzymes (New England Biolabs) for 12 h. The reactions were performed at 94°C for 1.5 min followed by 34 cycles of 30 s at 94°C, 30 s at the annealing temperature indicated above, 30 s at 72°C, and finally one cycle of 5 min at 72°C.

    Incubation:

    Article Title: Interleukin-27 as a Novel Biomarker for Early Cardiopulmonary Failure in Enterovirus 71-Infected Children with Central Nervous System Involvement
    Article Snippet: .. The primer sequences for the rs153109 SNP were F: 5′-TCAGTCAGTGACCAGGATCG-3′ and R: 5′-ACCAAGAAACCCCATCCTCT-3′, the annealing temperature was 58°C, and the PCR products were incubated with PaeR7I restriction enzymes (New England Biolabs, Ipswich, MA, USA) for 4 h. The primer sequences for rs17855750 were F: 5′-ATCTCGCCAGGAAGCTGCGC-3′ and R: 5′-CTGTTAGTGGGGGCCAGAAGGGA-3′, the annealing temperature was 62°C, and the PCR products were incubated with restriction enzymes BstUI restriction enzymes (New England Biolabs) for 8 h. The primer sequences for rs181206 were F: 5′-GCTTCAGCCCTTCCATGCCC-3′ and R: 5′-TCTACCTGGAAGCGGAGGTGCC-3′, the annealing temperature was 64°C, and the PCR products were incubated with FauI restriction enzymes (New England Biolabs) for 12 h. The reactions were performed at 94°C for 1.5 min followed by 34 cycles of 30 s at 94°C, 30 s at the annealing temperature indicated above, 30 s at 72°C, and finally one cycle of 5 min at 72°C. .. The obtained digestion products were visualized on a 4% agarose gel and stained with Gold View (SBS Genentech, Beijing, China).

    DNA Sequencing:

    Article Title: Interleukin-27 as a Novel Biomarker for Early Cardiopulmonary Failure in Enterovirus 71-Infected Children with Central Nervous System Involvement
    Article Snippet: The primer sequences for the rs153109 SNP were F: 5′-TCAGTCAGTGACCAGGATCG-3′ and R: 5′-ACCAAGAAACCCCATCCTCT-3′, the annealing temperature was 58°C, and the PCR products were incubated with PaeR7I restriction enzymes (New England Biolabs, Ipswich, MA, USA) for 4 h. The primer sequences for rs17855750 were F: 5′-ATCTCGCCAGGAAGCTGCGC-3′ and R: 5′-CTGTTAGTGGGGGCCAGAAGGGA-3′, the annealing temperature was 62°C, and the PCR products were incubated with restriction enzymes BstUI restriction enzymes (New England Biolabs) for 8 h. The primer sequences for rs181206 were F: 5′-GCTTCAGCCCTTCCATGCCC-3′ and R: 5′-TCTACCTGGAAGCGGAGGTGCC-3′, the annealing temperature was 64°C, and the PCR products were incubated with FauI restriction enzymes (New England Biolabs) for 12 h. The reactions were performed at 94°C for 1.5 min followed by 34 cycles of 30 s at 94°C, 30 s at the annealing temperature indicated above, 30 s at 72°C, and finally one cycle of 5 min at 72°C. .. To validate the method used in this study, PCR-amplified DNA samples from 45 cases (20% of all subjects) randomly selected subjects were examined by DNA sequencing at Invitrogen Biotechnology Company (Guangzhou, China) and the results were 100% concordant.

    Polymerase Chain Reaction:

    Article Title: Interleukin-27 as a Novel Biomarker for Early Cardiopulmonary Failure in Enterovirus 71-Infected Children with Central Nervous System Involvement
    Article Snippet: .. The primer sequences for the rs153109 SNP were F: 5′-TCAGTCAGTGACCAGGATCG-3′ and R: 5′-ACCAAGAAACCCCATCCTCT-3′, the annealing temperature was 58°C, and the PCR products were incubated with PaeR7I restriction enzymes (New England Biolabs, Ipswich, MA, USA) for 4 h. The primer sequences for rs17855750 were F: 5′-ATCTCGCCAGGAAGCTGCGC-3′ and R: 5′-CTGTTAGTGGGGGCCAGAAGGGA-3′, the annealing temperature was 62°C, and the PCR products were incubated with restriction enzymes BstUI restriction enzymes (New England Biolabs) for 8 h. The primer sequences for rs181206 were F: 5′-GCTTCAGCCCTTCCATGCCC-3′ and R: 5′-TCTACCTGGAAGCGGAGGTGCC-3′, the annealing temperature was 64°C, and the PCR products were incubated with FauI restriction enzymes (New England Biolabs) for 12 h. The reactions were performed at 94°C for 1.5 min followed by 34 cycles of 30 s at 94°C, 30 s at the annealing temperature indicated above, 30 s at 72°C, and finally one cycle of 5 min at 72°C. .. The obtained digestion products were visualized on a 4% agarose gel and stained with Gold View (SBS Genentech, Beijing, China).

    Staining:

    Article Title: Interleukin-27 as a Novel Biomarker for Early Cardiopulmonary Failure in Enterovirus 71-Infected Children with Central Nervous System Involvement
    Article Snippet: The primer sequences for the rs153109 SNP were F: 5′-TCAGTCAGTGACCAGGATCG-3′ and R: 5′-ACCAAGAAACCCCATCCTCT-3′, the annealing temperature was 58°C, and the PCR products were incubated with PaeR7I restriction enzymes (New England Biolabs, Ipswich, MA, USA) for 4 h. The primer sequences for rs17855750 were F: 5′-ATCTCGCCAGGAAGCTGCGC-3′ and R: 5′-CTGTTAGTGGGGGCCAGAAGGGA-3′, the annealing temperature was 62°C, and the PCR products were incubated with restriction enzymes BstUI restriction enzymes (New England Biolabs) for 8 h. The primer sequences for rs181206 were F: 5′-GCTTCAGCCCTTCCATGCCC-3′ and R: 5′-TCTACCTGGAAGCGGAGGTGCC-3′, the annealing temperature was 64°C, and the PCR products were incubated with FauI restriction enzymes (New England Biolabs) for 12 h. The reactions were performed at 94°C for 1.5 min followed by 34 cycles of 30 s at 94°C, 30 s at the annealing temperature indicated above, 30 s at 72°C, and finally one cycle of 5 min at 72°C. .. The primer sequences for the rs153109 SNP were F: 5′-TCAGTCAGTGACCAGGATCG-3′ and R: 5′-ACCAAGAAACCCCATCCTCT-3′, the annealing temperature was 58°C, and the PCR products were incubated with PaeR7I restriction enzymes (New England Biolabs, Ipswich, MA, USA) for 4 h. The primer sequences for rs17855750 were F: 5′-ATCTCGCCAGGAAGCTGCGC-3′ and R: 5′-CTGTTAGTGGGGGCCAGAAGGGA-3′, the annealing temperature was 62°C, and the PCR products were incubated with restriction enzymes BstUI restriction enzymes (New England Biolabs) for 8 h. The primer sequences for rs181206 were F: 5′-GCTTCAGCCCTTCCATGCCC-3′ and R: 5′-TCTACCTGGAAGCGGAGGTGCC-3′, the annealing temperature was 64°C, and the PCR products were incubated with FauI restriction enzymes (New England Biolabs) for 12 h. The reactions were performed at 94°C for 1.5 min followed by 34 cycles of 30 s at 94°C, 30 s at the annealing temperature indicated above, 30 s at 72°C, and finally one cycle of 5 min at 72°C.

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  • 94
    New England Biolabs paer7i restriction enzymes
    Paer7i Restriction Enzymes, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/paer7i restriction enzymes/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    paer7i restriction enzymes - by Bioz Stars, 2020-03
    94/100 stars
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