avrii (New England Biolabs)
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New England Biolabs manufactures this product 
95
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Name:
AvrII
Description:
AvrII 500 units
Catalog Number:
r0174l
Price:
290
Category:
Restriction Enzymes
Size:
500 units
|
Buy from Supplier |
Structured Review
New England Biolabs
avrii
AvrII 500 units
https://www.bioz.com/result/avrii/product/New England Biolabs
Average 95 stars, based on 1 article reviews
Price from $9.99 to $1999.99
AvrII 500 units
https://www.bioz.com/result/avrii/product/New England Biolabs
Average 95 stars, based on 1 article reviews
Price from $9.99 to $1999.99
avrii - by Bioz Stars,
2021-03
95/100 stars
Images
1) Product Images from "Pathogenicity and Immunogenicity of Recombinant Rabies Viruses Expressing the Lagos Bat Virus Matrix and Glycoprotein: Perspectives for a Pan-Lyssavirus Vaccine"
Article Title: Pathogenicity and Immunogenicity of Recombinant Rabies Viruses Expressing the Lagos Bat Virus Matrix and Glycoprotein: Perspectives for a Pan-Lyssavirus Vaccine
Journal: Tropical Medicine and Infectious Disease
doi: 10.3390/tropicalmed2030037
Figure Legend Snippet: Schematic representation of the construction of recombinant viruses. Restriction enzyme sites are indicated ( XmaI , PacI , AvrII , KpnI . BsiWI , AsiSI , NheI and AscI ). GAS represents the SPBN G gene with two amino acid substitutions (Asn 194 to Ser and Arg 333 to Glu). The following abbreviations were used: N, nucleoprotein; M, matrix protein; G, glycoprotein; L, RNA-dependent RNA polymerase. LBVM and LBVG represent the LBV M and G gene respectively.
Techniques Used: Recombinant
2) Product Images from "A novel approach to the generation of seamless constructs for plant transformation"
Article Title: A novel approach to the generation of seamless constructs for plant transformation
Journal: Plant Methods
doi: 10.1186/1746-4811-10-10
Figure Legend Snippet: Vector linearization and RE analysis of cloned gfp . A . pAUrumII in its circular (supercoiled) and LguI treated, linear state with the LguI -spacer removed next to pAUrumIII in its circular (supercoiled) and BaeI treated, linear state. The BaeI treated pAUrumIII also appears in a relaxed, circular state. The short BaeI -spacer is not visible. B . Restriction analysis for pAUrumII- gfp with NotI on plasmid DNA from 15 colonies. The correct construct is separated in two fragments of 2.6 kb and 1.5 kb, respectively. C . Restriction analysis for pAUrumIII- gfp with AvrII . The correct construct is separated in two fragments of 2.6 kb and 1.5 kb, respectively. The construct separated in a 2.6 kb and a 0.86 kb fragment is pAUrumIII. For all gels, L indicates the O’GeneRuler 1 kb DNA Ladder (Thermo Scientific).
Techniques Used: Plasmid Preparation, Clone Assay, Construct
3) Product Images from "CTAG-Containing Cleavage Site Profiling to Delineate Salmonella into Natural Clusters"
Article Title: CTAG-Containing Cleavage Site Profiling to Delineate Salmonella into Natural Clusters
Journal: PLoS ONE
doi: 10.1371/journal.pone.0103388
![Physical map comparison between S. gallinarum strains 287/91 and SARB21. The map of SARB21 was reported previously [21] ; here letter designations for the cleavage fragments of SARB21 have been changed according to the homologues in strain 287/91 for the convenience of comparison. Note that all XbaI, I-CeuI and AvrII (maps from top to bottom) cleavage sites are conserved in the two strains except the AvrII site between fragments F and J in 287/91 (open arrow), which is missing from SARB21. Lines with solid arrowheads at both ends indicate the ranges of genomic inversions via rrn -mediated recombination between the two strains and filled arrows indicate recombination sites that have resulted in the translocation of I-CeuI fragment D.](https://storage.googleapis.com/bioz_article_images/PMC4138082/pone.0103388.g004.jpg "... of comparison. Note that all XbaI, I-CeuI and AvrII (maps from top to bottom) cleavage sites are ...")
Figure Legend Snippet: Physical map comparison between S. gallinarum strains 287/91 and SARB21. The map of SARB21 was reported previously [21] ; here letter designations for the cleavage fragments of SARB21 have been changed according to the homologues in strain 287/91 for the convenience of comparison. Note that all XbaI, I-CeuI and AvrII (maps from top to bottom) cleavage sites are conserved in the two strains except the AvrII site between fragments F and J in 287/91 (open arrow), which is missing from SARB21. Lines with solid arrowheads at both ends indicate the ranges of genomic inversions via rrn -mediated recombination between the two strains and filled arrows indicate recombination sites that have resulted in the translocation of I-CeuI fragment D.
Techniques Used: Translocation Assay
Figure Legend Snippet: XbaI and AvrII cleavage patterns of S. gallinarum strains 287/91 and SARB21 after PFGE separation. (A) XbaI cleavage. Lanes: 1, SARB21; 2, 287/91; 3, λDNA as molecular size marker. (B) AvrII cleavage. Lanes: 1, λDNA as molecular size marker; 2, SARB21; 3, 287/91. Letter designations are for strain 287/91; the same letters are used for homologous fragments in strain SARB21. In the designation of fragments in SARB21, C′ means a fragment homologous to C in 287/91 but truncated on the right-hand part by genomic rearrangement, and ‘C means truncation on the left-hand part of the fragment.
Techniques Used: Marker
4) Product Images from "Evidence of Metabolic Switching and Implications for Food Safety from the Phenome(s) of Salmonella enterica Serovar Typhimurium DT104 Cultured at Selected Points across the Pork Production Food Chain"
Article Title: Evidence of Metabolic Switching and Implications for Food Safety from the Phenome(s) of Salmonella enterica Serovar Typhimurium DT104 Cultured at Selected Points across the Pork Production Food Chain
Journal: Applied and Environmental Microbiology
doi: 10.1128/AEM.01041-13
Figure Legend Snippet: (A) PFGE analysis of S . Typhimurium DT104 using XbaI and AvrII. Isolate groups were color identified for simplicity as follows, and the color codes were maintained in all of the assays: reference strain (white); group A, environmental (green); group B,
Techniques Used:
Related Articles
Activity Assay:Article Title: Heterologous Gene Expression from Transmissible Gastroenteritis Virus Replicon Particles Article Snippet: XI sites, allowing for directional assembly into a full-length replicon cDNA by in vitro ligation ( Serial deletions within the TGEV structural gene region were generated from the unique Pfl MI site at the very 3′ end of the GFP gene and extended for various distances toward the 3′ end of the genome (Fig. ). .. TGEV pFiGFP2( Pfl MI) was digested with Pfl MI and Generated:Article Title: Heterologous Gene Expression from Transmissible Gastroenteritis Virus Replicon Particles Article Snippet: XI sites, allowing for directional assembly into a full-length replicon cDNA by in vitro ligation ( Serial deletions within the TGEV structural gene region were generated from the unique Pfl MI site at the very 3′ end of the GFP gene and extended for various distances toward the 3′ end of the genome (Fig. ). .. TGEV pFiGFP2( Pfl MI) was digested with Pfl MI and Pulsed-Field Gel:Article Title: The Highly Virulent 2006 Norwegian EHEC O103:H25 Outbreak Strain Is Related to the 2011 German O104:H4 Outbreak Strain Article Snippet: Primers colE2 F and colE2 R ( ) and standard PCR conditions were used to generate a probe for plasmid hybridization. .. Pulsed-Field Gel Electrophoresis (PFGE) and plasmid isolation The E. coli isolates associated with the outbreak were analyzed by PFGE as described previously using the restriction enzymes Xba I and Article Title: Evidence of Metabolic Switching and Implications for Food Safety from the Phenome(s) of Salmonella enterica Serovar Typhimurium DT104 Cultured at Selected Points across the Pork Production Food Chain Article Snippet: .. Pulsed-field gel electrophoresis (PFGE) was performed using the restriction endonucleases XbaI and Plasmid Preparation:Article Title: The Highly Virulent 2006 Norwegian EHEC O103:H25 Outbreak Strain Is Related to the 2011 German O104:H4 Outbreak Strain Article Snippet: Primers colE2 F and colE2 R ( ) and standard PCR conditions were used to generate a probe for plasmid hybridization. .. Pulsed-Field Gel Electrophoresis (PFGE) and plasmid isolation The E. coli isolates associated with the outbreak were analyzed by PFGE as described previously using the restriction enzymes Xba I and Article Title: A novel approach to the generation of seamless constructs for plant transformation Article Snippet: After the 1 h shaking of the transformed bacteria, a 1:100 dilution was prepared in S.O.C. medium and 100 μL were spread on each of three LB agar plates (100 mg/L ampicillin, 100 mg/L X-gal) before incubation overnight at 37°C. .. Plasmid mini-preparations of overnight LB cultures (100 mg/L ampicillin) of randomly selected single white colonies were analyzed with REs NotI for pAUrumII-gfp , Isolation:Article Title: The Highly Virulent 2006 Norwegian EHEC O103:H25 Outbreak Strain Is Related to the 2011 German O104:H4 Outbreak Strain Article Snippet: Primers colE2 F and colE2 R ( ) and standard PCR conditions were used to generate a probe for plasmid hybridization. .. Pulsed-Field Gel Electrophoresis (PFGE) and plasmid isolation The E. coli isolates associated with the outbreak were analyzed by PFGE as described previously using the restriction enzymes Xba I and other:Article Title: Genomic Diversification among Archival Strains of Salmonella enterica Serovar Typhimurium LT7 Article Snippet: I- Ceu I, Avr II, and Spe I were purchased from Construct:Article Title: A novel approach to the generation of seamless constructs for plant transformation Article Snippet: After the 1 h shaking of the transformed bacteria, a 1:100 dilution was prepared in S.O.C. medium and 100 μL were spread on each of three LB agar plates (100 mg/L ampicillin, 100 mg/L X-gal) before incubation overnight at 37°C. .. Plasmid mini-preparations of overnight LB cultures (100 mg/L ampicillin) of randomly selected single white colonies were analyzed with REs NotI for pAUrumII-gfp , |
![TGEV-Rep( Avr II) VRPs. Transfected cultures displayed GFP expression at ∼18 h posttransfection (A) [TGEV-Rep( Avr II) pass 0]. Supernatants were harvested ∼36 h posttransfection and used to infect fresh cultures of ST cells for 1 h at room temperature. At ∼18 h postinfection, GFP expression was observed by fluorescent microscopy in these pass 1 cultures (C and D). (C) TGEV VRP production following coelectroporation with VEE-E RNAs [TGEV-Rep( Avr II) plus E protein transcripts pass 1]; (D) TGEV VRP production following infection with VEE VRPs encoding the E protein [TGEV-Rep( Avr II) plus VEE-TGEV(E) pass 1]. Passage of supernatants from cells transfected with TGEV-Rep( Avr II) transcripts without expression of the E protein in trans did not result in detectable GFP expression (B) [TGEV-Rep( Avr II) without E protein pass 1].](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC135785/bin/jv0321036005.jpg)
![Heterologous gene expression from TGEV replicon RNAs. (a) Cultures of BHK cells were transfected with TGEV replicon RNAs encoding GFP that contained ORF 3B and E (A) [TGEV-Rep( Avr II) transcripts] or ORF 3B, E, and M gene deletions (C). Alternatively, transcripts were treated with RNase prior to transfection into BHK cells (B and D) [TGEV-Rep( Avr II) DNA and TGEV-Rep( Eco NI) DNA only, respectively]. At ∼18 h posttransfection, GFP expression was observed by fluorescent microscopy. (b) Intracellular RNA was isolated from transfected cell cultures and used as a template for RT-PCR. Leader-containing GFP subgenomic transcripts were detected using a 5′ leader primer (TGEV-L) and 3′ primers located just downstream of the Avr II [(−)E5546] or Eco NI [M6400(−)] sites. Appropriately sized amplicons of ∼850 bp were generated, corresponding to transcripts encoding GFP. Cells transfected with TGEV-Rep( Avr II) (A) or TGEV-Rep( Eco NI) (B) RNA are shown. A 1-kb ladder is shown in both panels (lanes 1). Arrows indicate leader-containing GFP amplicons (lanes 2).](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC135785/bin/jv032103603a.jpg)
![Strategy to assemble TGEV-Rep( Avr II) VRPs. (a) In the full-length TGEV-Rep( Avr II) cDNA construct, ORF 3A has been replaced with GFP, and ORF 3B and the 5′ end of the E gene have been deleted. To produce packaged replicon particles, replicon RNA-transfected cells were infected with VEE VRPs expressing the TGEV E protein [VEE-TGEV(E)]. Alternatively, TGEV-Rep( Avr II) replicon RNAs can be coelectroporated with pVR21-E1-derived transcripts. TGEV VRPs should be released from cells that can be used as single-hit expression vectors. (b) Cultures of ST cells were infected with either wild-type (wt) TGEV alone or with TGEV and VEE VRPs expressing a G 1 Norwalk-like virus capsid (wt + VEE) at an MOI of 5 for 1 h at room temperature. The inocula were removed, and the cultures were incubated in complete medium at 37°C. Samples were harvested at the indicated times and assayed by plaque assay in ST cells.](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC135785/bin/jv032103604a.jpg)
