acci restriction enzyme  (New England Biolabs)


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    Name:
    AccI
    Description:
    AccI 5 000 units
    Catalog Number:
    r0161l
    Price:
    302
    Size:
    5 000 units
    Category:
    Restriction Enzymes
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    Structured Review

    New England Biolabs acci restriction enzyme
    AccI
    AccI 5 000 units
    https://www.bioz.com/result/acci restriction enzyme/product/New England Biolabs
    Average 90 stars, based on 402 article reviews
    Price from $9.99 to $1999.99
    acci restriction enzyme - by Bioz Stars, 2020-04
    90/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Functional Divergence of APETALA1 and FRUITFULL is due to Changes in both Regulation and Coding Sequence
    Article Snippet: .. Restriction digest with AccI [New England Biolabs (NEBs)] and BamHI (NEB) was performed on both the full-length FUL promoter clone in the pCR TOPO-XL vector and on PCR3 clones. .. Fragments of the appropriate length were excised from an agarose gel, purified using the QIAEX II Gel Extraction Kit (Qiagen), ligated using T4 DNA ligase (NEB), and sequenced in both directions.

    Amplification:

    Article Title: Cryptic Plutella species show deep divergence despite the capacity to hybridize
    Article Snippet: A 707 bp COI region was amplified using a combination of two primer pairs: (i) PxCOIF (5′ -TCAACAAATCATAAAGATATTGG- 3′ ) and PxCOIR (5′ -TAAACTTCAGGGTGACCAAAAAATCA- 3′ ), and (ii) PaCOIF (5′ -TCAACAAATCATAAGGATATTGG- 3′ ) and PaCOIR (5 ′ -TAAACCTCTGGATGGCCAAAAAATCA- 3′ ). .. PCR products were digested at 37 °C for 1 h with 1 unit of AccI (NEB) restriction enzyme with 2 µL Cutsmart Buffer in a 20 µL reaction.

    Article Title: Comparison of Direct Sequencing, Real-Time PCR-High Resolution Melt (PCR-HRM) and PCR-Restriction Fragment Length Polymorphism (PCR-RFLP) Analysis for Genotyping of Common Thiopurine Intolerant Variant Alleles NUDT15 c.415C > T and TPMT c.719A > G (TPMT*3C)
    Article Snippet: The target region of the NUDT15 and TPMT genes was amplified by PCR using the primers PCP-0023/24 and PCP-0027/28 with the stepdown PCR protocol as described above. .. Each TPMT *3C genotyping restriction digestion reaction contained 1.7 µL unpurified PCR product, 0.2 µL AccI restriction enzyme (New England Biolabs, Ipswich, MA, USA), 0.3 µL CutSmart Buffer (New England Biolabs, Ipswich, MA, USA) and nuclease-free water added up to 5 µL.

    Positive Control:

    Article Title: Meiotic association between Spo11 regulated by Rec102, Rec104 and Rec114
    Article Snippet: At the GAL2 locus, the purified genomic DNA was treated with AccI, BspHI, HhaI and Nt.AlwI (New England BioLabs) for several hours. .. Nt.AlwI was used as a positive control for SSB detection.

    Neutralization:

    Article Title: OxyR-dependent formation of DNA methylation patterns in OpvABOFF and OpvABON cell lineages of Salmonella enterica
    Article Snippet: A total of 16 μg of each DNA sample were digested with HaeIII and AccI (New England Biolabs, Ipswich, MA, USA), purified and divided into four fractions, three of which were subsequently digested with DpnI, MboI or Sau3AI (New England Biolabs). .. After digestion the samples were run in a 2% TAE-agarose gel at 100 V for 2 h. After electrophoresis, the DNA was denatured by treatment of the gel in acid conditions (0.25 M HCl, two washes 15 min each), followed by alkalinization (0.5 M NaOH, 1.5 M NaCl) and neutralization (0.5 M Tris, 1.5 M NaCl, pH 7.5; two washes, 30 min each).

    Construct:

    Article Title: Functional Divergence of APETALA1 and FRUITFULL is due to Changes in both Regulation and Coding Sequence
    Article Snippet: Paragraph title: Promoter Swap Constructs ... Restriction digest with AccI [New England Biolabs (NEBs)] and BamHI (NEB) was performed on both the full-length FUL promoter clone in the pCR TOPO-XL vector and on PCR3 clones.

    Electrophoresis:

    Article Title: Comparison of Direct Sequencing, Real-Time PCR-High Resolution Melt (PCR-HRM) and PCR-Restriction Fragment Length Polymorphism (PCR-RFLP) Analysis for Genotyping of Common Thiopurine Intolerant Variant Alleles NUDT15 c.415C > T and TPMT c.719A > G (TPMT*3C)
    Article Snippet: Each TPMT *3C genotyping restriction digestion reaction contained 1.7 µL unpurified PCR product, 0.2 µL AccI restriction enzyme (New England Biolabs, Ipswich, MA, USA), 0.3 µL CutSmart Buffer (New England Biolabs, Ipswich, MA, USA) and nuclease-free water added up to 5 µL. .. The digestion mix was incubated either at 65 °C (for TaaI digestion) or 37 °C (for AccI digestion) for 15 min on a thermal block (Eppendorf, Hamburg, Germany), and separated alongside 2.0 µL of undigested PCR product using a 2% agarose gel in 1× TBE buffer by electrophoresis at 100 V for 45 min.

    Article Title: Variable within- and between-Herd Diversity of CTX-M Cephalosporinase-Bearing Escherichia coli Isolates from Dairy Cattle
    Article Snippet: The plasmid content of each isolate was visualized by electrophoresis using a standard procedure ( ). .. Plasmids were characterized using restriction fragment analysis by digestion of 10 μl of extracted plasmid DNA ( ) overnight with 1 μl of AccI (New England BioLabs) at 37°C.

    Article Title: Two Novel Mutations of SCN9A (Nav1.7) are Associated with Partial Congenital Insensitivity to Pain
    Article Snippet: DNA-PCR products from exons 26B (577 bp product) and 17 (292 bp product) were digested with BsaJI and AccI, respectively, per manufacturer’s recommended conditions (New England Biolabs, Ipswich, MA). .. Digested products were subjected to electrophoresis on an 8% native polyacrylamide gel for 1.5 hours at 200V, and visualized by ethidium bromide staining.

    Article Title: OxyR-dependent formation of DNA methylation patterns in OpvABOFF and OpvABON cell lineages of Salmonella enterica
    Article Snippet: A total of 16 μg of each DNA sample were digested with HaeIII and AccI (New England Biolabs, Ipswich, MA, USA), purified and divided into four fractions, three of which were subsequently digested with DpnI, MboI or Sau3AI (New England Biolabs). .. After digestion the samples were run in a 2% TAE-agarose gel at 100 V for 2 h. After electrophoresis, the DNA was denatured by treatment of the gel in acid conditions (0.25 M HCl, two washes 15 min each), followed by alkalinization (0.5 M NaOH, 1.5 M NaCl) and neutralization (0.5 M Tris, 1.5 M NaCl, pH 7.5; two washes, 30 min each).

    Incubation:

    Article Title: Meiotic association between Spo11 regulated by Rec102, Rec104 and Rec114
    Article Snippet: After the agarose plugs were melted at 65°C for 10 min, S1 nuclease was added and the mixture was incubated for 30 min at 37°C ( , ). .. At the GAL2 locus, the purified genomic DNA was treated with AccI, BspHI, HhaI and Nt.AlwI (New England BioLabs) for several hours.

    Article Title: HPV prevalence and type distribution in Cypriot women with cervical cytological abnormalities
    Article Snippet: Reactions were incubated at 37 °C for 1 h. Digested products were electrophoretically separated on 2% agarose gels supplemented with ethidium bromide in parallel with a 100 bp DNA ladder (New England BioLabs, Ipswich, MA). .. In cases where the HPV types were unidentifiable due to the similarity of restriction profile, or a mixture of HPV types was detected, a second round of restriction digestion of PCR products was performed using the above protocol with the following restriction endonucleases: AccI, HaeIII, HincII, HindIII, SpeI, and SspI (all from New England BioLabs, Ipswich, MA), according to the manufacturer’s instructions.

    Article Title: Comparison of Direct Sequencing, Real-Time PCR-High Resolution Melt (PCR-HRM) and PCR-Restriction Fragment Length Polymorphism (PCR-RFLP) Analysis for Genotyping of Common Thiopurine Intolerant Variant Alleles NUDT15 c.415C > T and TPMT c.719A > G (TPMT*3C)
    Article Snippet: Each TPMT *3C genotyping restriction digestion reaction contained 1.7 µL unpurified PCR product, 0.2 µL AccI restriction enzyme (New England Biolabs, Ipswich, MA, USA), 0.3 µL CutSmart Buffer (New England Biolabs, Ipswich, MA, USA) and nuclease-free water added up to 5 µL. .. The digestion mix was incubated either at 65 °C (for TaaI digestion) or 37 °C (for AccI digestion) for 15 min on a thermal block (Eppendorf, Hamburg, Germany), and separated alongside 2.0 µL of undigested PCR product using a 2% agarose gel in 1× TBE buffer by electrophoresis at 100 V for 45 min.

    Gel Extraction:

    Article Title: Functional Divergence of APETALA1 and FRUITFULL is due to Changes in both Regulation and Coding Sequence
    Article Snippet: Restriction digest with AccI [New England Biolabs (NEBs)] and BamHI (NEB) was performed on both the full-length FUL promoter clone in the pCR TOPO-XL vector and on PCR3 clones. .. Fragments of the appropriate length were excised from an agarose gel, purified using the QIAEX II Gel Extraction Kit (Qiagen), ligated using T4 DNA ligase (NEB), and sequenced in both directions.

    Conjugation Assay:

    Article Title: Variable within- and between-Herd Diversity of CTX-M Cephalosporinase-Bearing Escherichia coli Isolates from Dairy Cattle
    Article Snippet: Conjugation experiments ( ) to establish the transmissibility of plasmids harboring bla CTX-M utilized wild-type E. coli donors with a rifampin- and nalidixic acid-resistant derivative of E. coli K-12 MG1655 as the recipient strain. .. Plasmids were characterized using restriction fragment analysis by digestion of 10 μl of extracted plasmid DNA ( ) overnight with 1 μl of AccI (New England BioLabs) at 37°C.

    Transfection:

    Article Title: Plasmodium IspD (2-C-Methyl-D-erythritol 4-Phosphate Cytidyltransferase), an Essential and Druggable Antimalarial Target
    Article Snippet: To assay the integration of pCAM-BSD-PfIspDcontrol , genomic DNA was harvested from wild-type 3D7 P. falciparum and from the continuously cultured pCAM-BSD-PfIspDcontrol transfectant, C1 (see above). .. These genomic DNA samples, along with pCAM-BSD-PfIspDKO plasmid, were digested with AccI and Pst I (New England Biolabs).

    Southern Blot:

    Article Title: OxyR-dependent formation of DNA methylation patterns in OpvABOFF and OpvABON cell lineages of Salmonella enterica
    Article Snippet: Paragraph title: Southern blot ... A total of 16 μg of each DNA sample were digested with HaeIII and AccI (New England Biolabs, Ipswich, MA, USA), purified and divided into four fractions, three of which were subsequently digested with DpnI, MboI or Sau3AI (New England Biolabs).

    Cell Culture:

    Article Title: Plasmodium IspD (2-C-Methyl-D-erythritol 4-Phosphate Cytidyltransferase), an Essential and Druggable Antimalarial Target
    Article Snippet: To assay the integration of pCAM-BSD-PfIspDcontrol , genomic DNA was harvested from wild-type 3D7 P. falciparum and from the continuously cultured pCAM-BSD-PfIspDcontrol transfectant, C1 (see above). .. These genomic DNA samples, along with pCAM-BSD-PfIspDKO plasmid, were digested with AccI and Pst I (New England Biolabs).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Bottleneck-mediated quasispecies restriction during spread of an RNA virus from inoculation site to brain
    Article Snippet: Paragraph title: RT-PCR Digestion Assay. ... PCR products were digested with NdeI, ApaLI, and AccI (NEB) at 37°C, ethanol-precipitated, and run on denaturing polyacrylamide gels as described in ref. .

    Generated:

    Article Title: Mutations in Membrin/GOSR2 Reveal Stringent Secretory Pathway Demands of Dendritic Growth and Synaptic Integrity
    Article Snippet: Drosophila Genetics and Phenotyping membrin 1,524 flies were previously generated in an ethyl methanesulfonate (EMS) screen and kindly shared by Mark Krasnow ( ). .. To control for potential genetic background effects, we outcrossed membrin 1,524 for five generations into an isogenic iso31 background by following an AccI (NEB) restriction site that is introduced by the nonsense mutation. daughterless -Gal4 (#55850), UAS-GCaMP6m (#42748), and nsyb -Gal4 (#51635) flies were obtained from the Bloomington Stock Center, and the membrin RNAi transgene was obtained from the Vienna Drosophila Resource Center (VDRC) (GD 44535) ( ).

    Sequencing:

    Article Title: Cryptic Plutella species show deep divergence despite the capacity to hybridize
    Article Snippet: PCR products were digested at 37 °C for 1 h with 1 unit of AccI (NEB) restriction enzyme with 2 µL Cutsmart Buffer in a 20 µL reaction. .. To examine mtDNA haplotypes, sequencing of the 707 bp COI amplicon was performed for 44 P. xylostella and 37 P. australiana individuals at the Australian Genome Research Facility (AGRF).

    Article Title: Variable within- and between-Herd Diversity of CTX-M Cephalosporinase-Bearing Escherichia coli Isolates from Dairy Cattle
    Article Snippet: Recipient acquisition of the expected plasmids and resistance genes was established with additional plasmid profiling, bla CTX-M PCR, and sequencing of the resulting amplicons recovered from transconjugants. .. Plasmids were characterized using restriction fragment analysis by digestion of 10 μl of extracted plasmid DNA ( ) overnight with 1 μl of AccI (New England BioLabs) at 37°C.

    Article Title: Functional Divergence of APETALA1 and FRUITFULL is due to Changes in both Regulation and Coding Sequence
    Article Snippet: PCR3 products, which consisted of 200 bp of the promoter and the coding sequence with the BamHI site, were cloned into pCR 2.1-TOPO and sequenced in both directions. .. Restriction digest with AccI [New England Biolabs (NEBs)] and BamHI (NEB) was performed on both the full-length FUL promoter clone in the pCR TOPO-XL vector and on PCR3 clones.

    Article Title: Mutations in Membrin/GOSR2 Reveal Stringent Secretory Pathway Demands of Dendritic Growth and Synaptic Integrity
    Article Snippet: This strain harbors a premature stop codon upstream of the membrin SNARE domain encoding sequence and therefore represents a null allele. .. To control for potential genetic background effects, we outcrossed membrin 1,524 for five generations into an isogenic iso31 background by following an AccI (NEB) restriction site that is introduced by the nonsense mutation. daughterless -Gal4 (#55850), UAS-GCaMP6m (#42748), and nsyb -Gal4 (#51635) flies were obtained from the Bloomington Stock Center, and the membrin RNAi transgene was obtained from the Vienna Drosophila Resource Center (VDRC) (GD 44535) ( ).

    DNA Extraction:

    Article Title: Cryptic Plutella species show deep divergence despite the capacity to hybridize
    Article Snippet: Paragraph title: DNA isolation and COI genotyping ... PCR products were digested at 37 °C for 1 h with 1 unit of AccI (NEB) restriction enzyme with 2 µL Cutsmart Buffer in a 20 µL reaction.

    Mutagenesis:

    Article Title: Two Novel Mutations of SCN9A (Nav1.7) are Associated with Partial Congenital Insensitivity to Pain
    Article Snippet: The putative mutations in exon 26B and exon 17 were confirmed by restriction enzyme digest using enzymes that cut one allele but not the other, due to the mutation. .. DNA-PCR products from exons 26B (577 bp product) and 17 (292 bp product) were digested with BsaJI and AccI, respectively, per manufacturer’s recommended conditions (New England Biolabs, Ipswich, MA).

    Article Title: Mutations in Membrin/GOSR2 Reveal Stringent Secretory Pathway Demands of Dendritic Growth and Synaptic Integrity
    Article Snippet: .. To control for potential genetic background effects, we outcrossed membrin 1,524 for five generations into an isogenic iso31 background by following an AccI (NEB) restriction site that is introduced by the nonsense mutation. daughterless -Gal4 (#55850), UAS-GCaMP6m (#42748), and nsyb -Gal4 (#51635) flies were obtained from the Bloomington Stock Center, and the membrin RNAi transgene was obtained from the Vienna Drosophila Resource Center (VDRC) (GD 44535) ( ). ..

    Isolation:

    Article Title: OxyR-dependent formation of DNA methylation patterns in OpvABOFF and OpvABON cell lineages of Salmonella enterica
    Article Snippet: Southern blot Genomic DNA was isolated by phenol extraction and ethanol precipitation from stationary cultures in LB (O.D.600 ∼4). .. A total of 16 μg of each DNA sample were digested with HaeIII and AccI (New England Biolabs, Ipswich, MA, USA), purified and divided into four fractions, three of which were subsequently digested with DpnI, MboI or Sau3AI (New England Biolabs).

    Purification:

    Article Title: Meiotic association between Spo11 regulated by Rec102, Rec104 and Rec114
    Article Snippet: .. At the GAL2 locus, the purified genomic DNA was treated with AccI, BspHI, HhaI and Nt.AlwI (New England BioLabs) for several hours. .. Nt.AlwI was used as a positive control for SSB detection.

    Article Title: Functional Divergence of APETALA1 and FRUITFULL is due to Changes in both Regulation and Coding Sequence
    Article Snippet: Restriction digest with AccI [New England Biolabs (NEBs)] and BamHI (NEB) was performed on both the full-length FUL promoter clone in the pCR TOPO-XL vector and on PCR3 clones. .. Fragments of the appropriate length were excised from an agarose gel, purified using the QIAEX II Gel Extraction Kit (Qiagen), ligated using T4 DNA ligase (NEB), and sequenced in both directions.

    Article Title: OxyR-dependent formation of DNA methylation patterns in OpvABOFF and OpvABON cell lineages of Salmonella enterica
    Article Snippet: .. A total of 16 μg of each DNA sample were digested with HaeIII and AccI (New England Biolabs, Ipswich, MA, USA), purified and divided into four fractions, three of which were subsequently digested with DpnI, MboI or Sau3AI (New England Biolabs). .. After digestion the samples were run in a 2% TAE-agarose gel at 100 V for 2 h. After electrophoresis, the DNA was denatured by treatment of the gel in acid conditions (0.25 M HCl, two washes 15 min each), followed by alkalinization (0.5 M NaOH, 1.5 M NaCl) and neutralization (0.5 M Tris, 1.5 M NaCl, pH 7.5; two washes, 30 min each).

    Polymerase Chain Reaction:

    Article Title: Bottleneck-mediated quasispecies restriction during spread of an RNA virus from inoculation site to brain
    Article Snippet: .. PCR products were digested with NdeI, ApaLI, and AccI (NEB) at 37°C, ethanol-precipitated, and run on denaturing polyacrylamide gels as described in ref. . .. We thank Peter Sarnow for helpful comments on the manuscript and Shane Crotty and Raul Andino for the provision of PVR mice.

    Article Title: HPV prevalence and type distribution in Cypriot women with cervical cytological abnormalities
    Article Snippet: .. In cases where the HPV types were unidentifiable due to the similarity of restriction profile, or a mixture of HPV types was detected, a second round of restriction digestion of PCR products was performed using the above protocol with the following restriction endonucleases: AccI, HaeIII, HincII, HindIII, SpeI, and SspI (all from New England BioLabs, Ipswich, MA), according to the manufacturer’s instructions. ..

    Article Title: Cryptic Plutella species show deep divergence despite the capacity to hybridize
    Article Snippet: .. PCR products were digested at 37 °C for 1 h with 1 unit of AccI (NEB) restriction enzyme with 2 µL Cutsmart Buffer in a 20 µL reaction. ..

    Article Title: Comparison of Direct Sequencing, Real-Time PCR-High Resolution Melt (PCR-HRM) and PCR-Restriction Fragment Length Polymorphism (PCR-RFLP) Analysis for Genotyping of Common Thiopurine Intolerant Variant Alleles NUDT15 c.415C > T and TPMT c.719A > G (TPMT*3C)
    Article Snippet: .. Each TPMT *3C genotyping restriction digestion reaction contained 1.7 µL unpurified PCR product, 0.2 µL AccI restriction enzyme (New England Biolabs, Ipswich, MA, USA), 0.3 µL CutSmart Buffer (New England Biolabs, Ipswich, MA, USA) and nuclease-free water added up to 5 µL. .. The digestion mix was incubated either at 65 °C (for TaaI digestion) or 37 °C (for AccI digestion) for 15 min on a thermal block (Eppendorf, Hamburg, Germany), and separated alongside 2.0 µL of undigested PCR product using a 2% agarose gel in 1× TBE buffer by electrophoresis at 100 V for 45 min.

    Article Title: Variable within- and between-Herd Diversity of CTX-M Cephalosporinase-Bearing Escherichia coli Isolates from Dairy Cattle
    Article Snippet: Individual plasmids were classified according to a PCR-based replicon typing procedure (PRT) that detects 18 replicon types based on incompatibility group loci ( , ) by using boiled lysate as template DNA. .. Plasmids were characterized using restriction fragment analysis by digestion of 10 μl of extracted plasmid DNA ( ) overnight with 1 μl of AccI (New England BioLabs) at 37°C.

    Article Title: Functional Divergence of APETALA1 and FRUITFULL is due to Changes in both Regulation and Coding Sequence
    Article Snippet: .. Restriction digest with AccI [New England Biolabs (NEBs)] and BamHI (NEB) was performed on both the full-length FUL promoter clone in the pCR TOPO-XL vector and on PCR3 clones. .. Fragments of the appropriate length were excised from an agarose gel, purified using the QIAEX II Gel Extraction Kit (Qiagen), ligated using T4 DNA ligase (NEB), and sequenced in both directions.

    Article Title: Subtypes of the Plasmid-Encoded Serine Protease EspP in Shiga Toxin-Producing Escherichia coli: Distribution, Secretion, and Proteolytic Activity ▿
    Article Snippet: .. Five hundred nanograms of the PCR product created with primers espPlong-1 and espPlong-2 (Table ) was digested with 5 units of AccI, DraI, RsaI, or SspI (New England Biolabs, Frankfurt, Germany) according to the manufacturer's instructions. .. Restriction fragments were separated on a 1.2% (wt/vol) agarose gel and visualized by staining with ethidium bromide.

    Blocking Assay:

    Article Title: Comparison of Direct Sequencing, Real-Time PCR-High Resolution Melt (PCR-HRM) and PCR-Restriction Fragment Length Polymorphism (PCR-RFLP) Analysis for Genotyping of Common Thiopurine Intolerant Variant Alleles NUDT15 c.415C > T and TPMT c.719A > G (TPMT*3C)
    Article Snippet: Each TPMT *3C genotyping restriction digestion reaction contained 1.7 µL unpurified PCR product, 0.2 µL AccI restriction enzyme (New England Biolabs, Ipswich, MA, USA), 0.3 µL CutSmart Buffer (New England Biolabs, Ipswich, MA, USA) and nuclease-free water added up to 5 µL. .. The digestion mix was incubated either at 65 °C (for TaaI digestion) or 37 °C (for AccI digestion) for 15 min on a thermal block (Eppendorf, Hamburg, Germany), and separated alongside 2.0 µL of undigested PCR product using a 2% agarose gel in 1× TBE buffer by electrophoresis at 100 V for 45 min.

    Lysis:

    Article Title: Meiotic association between Spo11 regulated by Rec102, Rec104 and Rec114
    Article Snippet: After cells were spheroplasted with Zymolyase-20T (MP Biomedicals, Inc.) in spheroplast buffer (1% 2-mercaptoethanol, 1 M sorbitol, 0.1 M EDTA [pH 8.0]), they were lysed and digested in lysis buffer (50 mM EDTA [pH 8.0], 50 mM Tris [pH 8.0], 0.5% SDS, 200 µg of proteinase K). .. At the GAL2 locus, the purified genomic DNA was treated with AccI, BspHI, HhaI and Nt.AlwI (New England BioLabs) for several hours.

    Plasmid Preparation:

    Article Title: Variable within- and between-Herd Diversity of CTX-M Cephalosporinase-Bearing Escherichia coli Isolates from Dairy Cattle
    Article Snippet: .. Plasmids were characterized using restriction fragment analysis by digestion of 10 μl of extracted plasmid DNA ( ) overnight with 1 μl of AccI (New England BioLabs) at 37°C. .. PCR, utilizing previously reported primer sets, was used to detect bla CTX-M and to screen for other classes of beta-lactamase resistance genes, including CMY-2, TEM, SHV, and OXA ( , , , ).

    Article Title: Functional Divergence of APETALA1 and FRUITFULL is due to Changes in both Regulation and Coding Sequence
    Article Snippet: .. Restriction digest with AccI [New England Biolabs (NEBs)] and BamHI (NEB) was performed on both the full-length FUL promoter clone in the pCR TOPO-XL vector and on PCR3 clones. .. Fragments of the appropriate length were excised from an agarose gel, purified using the QIAEX II Gel Extraction Kit (Qiagen), ligated using T4 DNA ligase (NEB), and sequenced in both directions.

    Article Title: Plasmodium IspD (2-C-Methyl-D-erythritol 4-Phosphate Cytidyltransferase), an Essential and Druggable Antimalarial Target
    Article Snippet: .. These genomic DNA samples, along with pCAM-BSD-PfIspDKO plasmid, were digested with AccI and Pst I (New England Biolabs). ..

    Agarose Gel Electrophoresis:

    Article Title: Comparison of Direct Sequencing, Real-Time PCR-High Resolution Melt (PCR-HRM) and PCR-Restriction Fragment Length Polymorphism (PCR-RFLP) Analysis for Genotyping of Common Thiopurine Intolerant Variant Alleles NUDT15 c.415C > T and TPMT c.719A > G (TPMT*3C)
    Article Snippet: Each TPMT *3C genotyping restriction digestion reaction contained 1.7 µL unpurified PCR product, 0.2 µL AccI restriction enzyme (New England Biolabs, Ipswich, MA, USA), 0.3 µL CutSmart Buffer (New England Biolabs, Ipswich, MA, USA) and nuclease-free water added up to 5 µL. .. The digestion mix was incubated either at 65 °C (for TaaI digestion) or 37 °C (for AccI digestion) for 15 min on a thermal block (Eppendorf, Hamburg, Germany), and separated alongside 2.0 µL of undigested PCR product using a 2% agarose gel in 1× TBE buffer by electrophoresis at 100 V for 45 min.

    Article Title: Functional Divergence of APETALA1 and FRUITFULL is due to Changes in both Regulation and Coding Sequence
    Article Snippet: Restriction digest with AccI [New England Biolabs (NEBs)] and BamHI (NEB) was performed on both the full-length FUL promoter clone in the pCR TOPO-XL vector and on PCR3 clones. .. Fragments of the appropriate length were excised from an agarose gel, purified using the QIAEX II Gel Extraction Kit (Qiagen), ligated using T4 DNA ligase (NEB), and sequenced in both directions.

    Article Title: Subtypes of the Plasmid-Encoded Serine Protease EspP in Shiga Toxin-Producing Escherichia coli: Distribution, Secretion, and Proteolytic Activity ▿
    Article Snippet: Five hundred nanograms of the PCR product created with primers espPlong-1 and espPlong-2 (Table ) was digested with 5 units of AccI, DraI, RsaI, or SspI (New England Biolabs, Frankfurt, Germany) according to the manufacturer's instructions. .. Restriction fragments were separated on a 1.2% (wt/vol) agarose gel and visualized by staining with ethidium bromide.

    Ethanol Precipitation:

    Article Title: OxyR-dependent formation of DNA methylation patterns in OpvABOFF and OpvABON cell lineages of Salmonella enterica
    Article Snippet: Southern blot Genomic DNA was isolated by phenol extraction and ethanol precipitation from stationary cultures in LB (O.D.600 ∼4). .. A total of 16 μg of each DNA sample were digested with HaeIII and AccI (New England Biolabs, Ipswich, MA, USA), purified and divided into four fractions, three of which were subsequently digested with DpnI, MboI or Sau3AI (New England Biolabs).

    Staining:

    Article Title: Two Novel Mutations of SCN9A (Nav1.7) are Associated with Partial Congenital Insensitivity to Pain
    Article Snippet: DNA-PCR products from exons 26B (577 bp product) and 17 (292 bp product) were digested with BsaJI and AccI, respectively, per manufacturer’s recommended conditions (New England Biolabs, Ipswich, MA). .. Digested products were subjected to electrophoresis on an 8% native polyacrylamide gel for 1.5 hours at 200V, and visualized by ethidium bromide staining.

    Article Title: Subtypes of the Plasmid-Encoded Serine Protease EspP in Shiga Toxin-Producing Escherichia coli: Distribution, Secretion, and Proteolytic Activity ▿
    Article Snippet: Five hundred nanograms of the PCR product created with primers espPlong-1 and espPlong-2 (Table ) was digested with 5 units of AccI, DraI, RsaI, or SspI (New England Biolabs, Frankfurt, Germany) according to the manufacturer's instructions. .. Restriction fragments were separated on a 1.2% (wt/vol) agarose gel and visualized by staining with ethidium bromide.

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  • 90
    New England Biolabs acci restriction enzyme
    Acci Restriction Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/acci restriction enzyme/product/New England Biolabs
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    acci restriction enzyme - by Bioz Stars, 2020-04
    90/100 stars
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