bcli (New England Biolabs)
New England Biolabs is a verified supplier 
New England Biolabs manufactures this product 
94
|
Name:
BclI
Description:
Catalog Number:
R0160
Price:
62
Category:
Restriction Enzymes
Applications:
DNA Manipulation
Size:
3000 units
|
Buy from Supplier |
Structured Review
New England Biolabs
bcli
https://www.bioz.com/result/bcli/product/New England Biolabs
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
https://www.bioz.com/result/bcli/product/New England Biolabs
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
bcli - by Bioz Stars,
2021-08
94/100 stars
Images
1) Product Images from "Herpesvirus telomeric repeats facilitate genomic integration into host telomeres and mobilization of viral DNA during reactivation"
Article Title: Herpesvirus telomeric repeats facilitate genomic integration into host telomeres and mobilization of viral DNA during reactivation
Journal: The Journal of Experimental Medicine
doi: 10.1084/jem.20101402
Figure Legend Snippet: Integration does not occur in host telomeres in the absence of viral TMRs. (A) Schematic representation and corresponding PFGE and Southern blot analysis of LCL DNA digested with SfiI. Fragment sizes generated by SfiI digestion of integrated and nonintegrated MDV genomes are depicted and sizes are given. The size of the linear MDV genome observed during lytic replication is indicated by an arrow. Results are representative of three independent Southern blot analyses. (B) Southern blotting of DNA of LCL derived from animals infected with vTE1 and digested with BclI. Potential intragenomic and telomeric integration sites are indicated. Results are representative of three independent Southern blot analyses. (C) Quantification of MDV copies in tumor cells. Results are shown as mean herpesvirus genome copies detected by the TR L probe relative to B2M in three independent experiments. The data are shown relative to LCL CU482 derived from a vRB-1B–infected chicken with standard deviations (error bars).
Techniques Used: Southern Blot, Generated, Derivative Assay, Infection
2) Product Images from "Simple CRISPR-Cas9 Genome Editing in S. cerevisiae"
Article Title: Simple CRISPR-Cas9 Genome Editing in S. cerevisiae
Journal: Current protocols in molecular biology
doi: 10.1002/cpmb.110
Figure Legend Snippet: Experimental strategy for cloning user-designed guide sequence into sgRNA expression cassette in pML104 or pML107. The BclI site is located at the 3’ end of the pSNR52 promoter, which is used to drive sgRNA expression in yeast. The SwaI site is located in the structural portion of the sgRNA. SwaI and BclI ).
Techniques Used: Clone Assay, Sequencing, Expressing
Figure Legend Snippet: Plasmid maps of the sgRNA/Cas9 expression vectors pML104 ( URA3 marker) and pML107 ( LEU2 marker). Both plasmids are yeast/ E. coli shuttle vectors containing an ampicillin resistance (AmpR) marker, and a yeast 2 micron (2μ) origin of replication. Both vectors contain a Cas9 expression cassette and an sgRNA expression cassette, which contains unique BclI and SwaI restriction sites, to facilitate directional cloning of the user-designed guide sequence into the sgRNA expression cassette.
Techniques Used: Plasmid Preparation, Expressing, Marker, Clone Assay, Sequencing
3) Product Images from "Identification of Rv3852 as an Agrimophol-Binding Protein in Mycobacterium tuberculosis"
Article Title: Identification of Rv3852 as an Agrimophol-Binding Protein in Mycobacterium tuberculosis
Journal: PLoS ONE
doi: 10.1371/journal.pone.0126211
Figure Legend Snippet: Construction of rv3852 knockout Mtb and verification by Southern blot and PCR. (A) Upper panel displays the genetic organization of the rv3852 region in Mtb (WT), Lower panel displays the same region with replacement of rv3852 by hygromycin resistance gene in rv3852 knockout Mtb (KO). Filled rectangle indicates the location of probe used in the Southern blot. Sites of digestion by BclI on genomic DNA from WT and KO as well as the sizes of the generated DNA fragment (a and b) are demonstrated under each panel. PCR products from genomic DNA from WT (1 and 2) and KO (3 and 4) are denoted. (B) Left, Southern blot of BclI digested genomic DNA from WT and KO. Calculated sizes of the fragments hybridizing with the probe were 3714 bp (WT) and 2916 bp (KO) as indicated in A. Right, PCR products from genomic DNA from WT and KO. The calculated sizes of the PCR products were 902 (Lane 1), 860 (Lane 2) for WT and 1322 (Lane 3), 1560 bp (Lane 4) for KO as indicated in A.
Techniques Used: Knock-Out, Southern Blot, Polymerase Chain Reaction, Generated
Related Articles
Lysis:Article Title: Herpesvirus telomeric repeats facilitate genomic integration into host telomeres and mobilization of viral DNA during reactivation Article Snippet: .. 1 × 107 /ml LCLs were embedded in 1% agarose and digested at 50°C for 48 h hours in lysis buffer (0.5 M EDTA and 1% wt/vol N -laurylsarcosine) containing 1 mg/ml proteinase K. Proteinase K was inactivated with 0.01 mM phenylmethanesulfonyl fluoride, and agarose plugs were digested with either SfiI or Amplification:Article Title: An association between the PTGS2 rs5275 polymorphism and colorectal cancer risk in families with inherited non-syndromic predisposition Article Snippet: .. For PIRA assays, 50 ng of template DNA was amplified (in duplicate) and resultant PCR products digested overnight using Polymerase Chain Reaction:Article Title: An association between the PTGS2 rs5275 polymorphism and colorectal cancer risk in families with inherited non-syndromic predisposition Article Snippet: .. For PIRA assays, 50 ng of template DNA was amplified (in duplicate) and resultant PCR products digested overnight using Plasmid Preparation:Article Title: Decoding the chromatin proteome of a single genomic locus by DNA sequencing Article Snippet: .. Preparation of the Cas9/gRNA vector The Cas9 and gRNA expression vector pML104 (Addgene plasmid number 67638 [ ]) was used to introduce a double-strand break at the ADE2 locus, 57 bp into the coding sequence. pML104 was isolated from dam-INV110 Escherichia coli (ThermoFisher number C717103) and digested with SwaI (NEB number R0604) and Expressing:Article Title: Decoding the chromatin proteome of a single genomic locus by DNA sequencing Article Snippet: .. Preparation of the Cas9/gRNA vector The Cas9 and gRNA expression vector pML104 (Addgene plasmid number 67638 [ ]) was used to introduce a double-strand break at the ADE2 locus, 57 bp into the coding sequence. pML104 was isolated from dam-INV110 Escherichia coli (ThermoFisher number C717103) and digested with SwaI (NEB number R0604) and Introduce:Article Title: Decoding the chromatin proteome of a single genomic locus by DNA sequencing Article Snippet: .. Preparation of the Cas9/gRNA vector The Cas9 and gRNA expression vector pML104 (Addgene plasmid number 67638 [ ]) was used to introduce a double-strand break at the ADE2 locus, 57 bp into the coding sequence. pML104 was isolated from dam-INV110 Escherichia coli (ThermoFisher number C717103) and digested with SwaI (NEB number R0604) and Sequencing:Article Title: Decoding the chromatin proteome of a single genomic locus by DNA sequencing Article Snippet: .. Preparation of the Cas9/gRNA vector The Cas9 and gRNA expression vector pML104 (Addgene plasmid number 67638 [ ]) was used to introduce a double-strand break at the ADE2 locus, 57 bp into the coding sequence. pML104 was isolated from dam-INV110 Escherichia coli (ThermoFisher number C717103) and digested with SwaI (NEB number R0604) and Isolation:Article Title: Decoding the chromatin proteome of a single genomic locus by DNA sequencing Article Snippet: .. Preparation of the Cas9/gRNA vector The Cas9 and gRNA expression vector pML104 (Addgene plasmid number 67638 [ ]) was used to introduce a double-strand break at the ADE2 locus, 57 bp into the coding sequence. pML104 was isolated from dam-INV110 Escherichia coli (ThermoFisher number C717103) and digested with SwaI (NEB number R0604) and Southern Blot:Article Title: Identification of Rv3852 as an Agrimophol-Binding Protein in Mycobacterium tuberculosis Article Snippet: .. For Southern blot analysis, genomic DNA from wild type Mtb and hygromycin resistant Mtb were digested by Agarose Gel Electrophoresis:Article Title: Identification of Rv3852 as an Agrimophol-Binding Protein in Mycobacterium tuberculosis Article Snippet: .. For Southern blot analysis, genomic DNA from wild type Mtb and hygromycin resistant Mtb were digested by Incubation:Article Title: New Vectors for Simple and Streamlined CRISPR-Cas9 Genome Editing in Saccharomyces cerevisiae Article Snippet: .. |