bcli  (New England Biolabs)


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    Name:
    BclI
    Description:

    Catalog Number:
    R0160
    Price:
    62
    Category:
    Restriction Enzymes
    Applications:
    DNA Manipulation
    Size:
    3000 units
    Buy from Supplier


    Structured Review

    New England Biolabs bcli
    BclI

    https://www.bioz.com/result/bcli/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bcli - by Bioz Stars, 2021-08
    94/100 stars

    Images

    1) Product Images from "Herpesvirus telomeric repeats facilitate genomic integration into host telomeres and mobilization of viral DNA during reactivation"

    Article Title: Herpesvirus telomeric repeats facilitate genomic integration into host telomeres and mobilization of viral DNA during reactivation

    Journal: The Journal of Experimental Medicine

    doi: 10.1084/jem.20101402

    Integration does not occur in host telomeres in the absence of viral TMRs. (A) Schematic representation and corresponding PFGE and Southern blot analysis of LCL DNA digested with SfiI. Fragment sizes generated by SfiI digestion of integrated and nonintegrated MDV genomes are depicted and sizes are given. The size of the linear MDV genome observed during lytic replication is indicated by an arrow. Results are representative of three independent Southern blot analyses. (B) Southern blotting of DNA of LCL derived from animals infected with vTE1 and digested with BclI. Potential intragenomic and telomeric integration sites are indicated. Results are representative of three independent Southern blot analyses. (C) Quantification of MDV copies in tumor cells. Results are shown as mean herpesvirus genome copies detected by the TR L probe relative to B2M in three independent experiments. The data are shown relative to LCL CU482 derived from a vRB-1B–infected chicken with standard deviations (error bars).
    Figure Legend Snippet: Integration does not occur in host telomeres in the absence of viral TMRs. (A) Schematic representation and corresponding PFGE and Southern blot analysis of LCL DNA digested with SfiI. Fragment sizes generated by SfiI digestion of integrated and nonintegrated MDV genomes are depicted and sizes are given. The size of the linear MDV genome observed during lytic replication is indicated by an arrow. Results are representative of three independent Southern blot analyses. (B) Southern blotting of DNA of LCL derived from animals infected with vTE1 and digested with BclI. Potential intragenomic and telomeric integration sites are indicated. Results are representative of three independent Southern blot analyses. (C) Quantification of MDV copies in tumor cells. Results are shown as mean herpesvirus genome copies detected by the TR L probe relative to B2M in three independent experiments. The data are shown relative to LCL CU482 derived from a vRB-1B–infected chicken with standard deviations (error bars).

    Techniques Used: Southern Blot, Generated, Derivative Assay, Infection

    2) Product Images from "Simple CRISPR-Cas9 Genome Editing in S. cerevisiae"

    Article Title: Simple CRISPR-Cas9 Genome Editing in S. cerevisiae

    Journal: Current protocols in molecular biology

    doi: 10.1002/cpmb.110

    Experimental strategy for cloning user-designed guide sequence into sgRNA expression cassette in pML104 or pML107. The BclI site is located at the 3’ end of the pSNR52 promoter, which is used to drive sgRNA expression in yeast. The SwaI site is located in the structural portion of the sgRNA. SwaI and BclI ).
    Figure Legend Snippet: Experimental strategy for cloning user-designed guide sequence into sgRNA expression cassette in pML104 or pML107. The BclI site is located at the 3’ end of the pSNR52 promoter, which is used to drive sgRNA expression in yeast. The SwaI site is located in the structural portion of the sgRNA. SwaI and BclI ).

    Techniques Used: Clone Assay, Sequencing, Expressing

    Plasmid maps of the sgRNA/Cas9 expression vectors pML104 ( URA3 marker) and pML107 ( LEU2 marker). Both plasmids are yeast/ E. coli shuttle vectors containing an ampicillin resistance (AmpR) marker, and a yeast 2 micron (2μ) origin of replication. Both vectors contain a Cas9 expression cassette and an sgRNA expression cassette, which contains unique BclI and SwaI restriction sites, to facilitate directional cloning of the user-designed guide sequence into the sgRNA expression cassette.
    Figure Legend Snippet: Plasmid maps of the sgRNA/Cas9 expression vectors pML104 ( URA3 marker) and pML107 ( LEU2 marker). Both plasmids are yeast/ E. coli shuttle vectors containing an ampicillin resistance (AmpR) marker, and a yeast 2 micron (2μ) origin of replication. Both vectors contain a Cas9 expression cassette and an sgRNA expression cassette, which contains unique BclI and SwaI restriction sites, to facilitate directional cloning of the user-designed guide sequence into the sgRNA expression cassette.

    Techniques Used: Plasmid Preparation, Expressing, Marker, Clone Assay, Sequencing

    3) Product Images from "Identification of Rv3852 as an Agrimophol-Binding Protein in Mycobacterium tuberculosis"

    Article Title: Identification of Rv3852 as an Agrimophol-Binding Protein in Mycobacterium tuberculosis

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0126211

    Construction of rv3852 knockout Mtb and verification by Southern blot and PCR. (A) Upper panel displays the genetic organization of the rv3852 region in Mtb (WT), Lower panel displays the same region with replacement of rv3852 by hygromycin resistance gene in rv3852 knockout Mtb (KO). Filled rectangle indicates the location of probe used in the Southern blot. Sites of digestion by BclI on genomic DNA from WT and KO as well as the sizes of the generated DNA fragment (a and b) are demonstrated under each panel. PCR products from genomic DNA from WT (1 and 2) and KO (3 and 4) are denoted. (B) Left, Southern blot of BclI digested genomic DNA from WT and KO. Calculated sizes of the fragments hybridizing with the probe were 3714 bp (WT) and 2916 bp (KO) as indicated in A. Right, PCR products from genomic DNA from WT and KO. The calculated sizes of the PCR products were 902 (Lane 1), 860 (Lane 2) for WT and 1322 (Lane 3), 1560 bp (Lane 4) for KO as indicated in A.
    Figure Legend Snippet: Construction of rv3852 knockout Mtb and verification by Southern blot and PCR. (A) Upper panel displays the genetic organization of the rv3852 region in Mtb (WT), Lower panel displays the same region with replacement of rv3852 by hygromycin resistance gene in rv3852 knockout Mtb (KO). Filled rectangle indicates the location of probe used in the Southern blot. Sites of digestion by BclI on genomic DNA from WT and KO as well as the sizes of the generated DNA fragment (a and b) are demonstrated under each panel. PCR products from genomic DNA from WT (1 and 2) and KO (3 and 4) are denoted. (B) Left, Southern blot of BclI digested genomic DNA from WT and KO. Calculated sizes of the fragments hybridizing with the probe were 3714 bp (WT) and 2916 bp (KO) as indicated in A. Right, PCR products from genomic DNA from WT and KO. The calculated sizes of the PCR products were 902 (Lane 1), 860 (Lane 2) for WT and 1322 (Lane 3), 1560 bp (Lane 4) for KO as indicated in A.

    Techniques Used: Knock-Out, Southern Blot, Polymerase Chain Reaction, Generated

    Related Articles

    Lysis:

    Article Title: Herpesvirus telomeric repeats facilitate genomic integration into host telomeres and mobilization of viral DNA during reactivation
    Article Snippet: .. 1 × 107 /ml LCLs were embedded in 1% agarose and digested at 50°C for 48 h hours in lysis buffer (0.5 M EDTA and 1% wt/vol N -laurylsarcosine) containing 1 mg/ml proteinase K. Proteinase K was inactivated with 0.01 mM phenylmethanesulfonyl fluoride, and agarose plugs were digested with either SfiI or BclI (New England Biolabs) overnight according to the manufacturer’s instructions. ..

    Amplification:

    Article Title: An association between the PTGS2 rs5275 polymorphism and colorectal cancer risk in families with inherited non-syndromic predisposition
    Article Snippet: .. For PIRA assays, 50 ng of template DNA was amplified (in duplicate) and resultant PCR products digested overnight using BclI (New England Biolabs Inc., Beverly, MA, USA). ..

    Polymerase Chain Reaction:

    Article Title: An association between the PTGS2 rs5275 polymorphism and colorectal cancer risk in families with inherited non-syndromic predisposition
    Article Snippet: .. For PIRA assays, 50 ng of template DNA was amplified (in duplicate) and resultant PCR products digested overnight using BclI (New England Biolabs Inc., Beverly, MA, USA). ..

    Plasmid Preparation:

    Article Title: Decoding the chromatin proteome of a single genomic locus by DNA sequencing
    Article Snippet: .. Preparation of the Cas9/gRNA vector The Cas9 and gRNA expression vector pML104 (Addgene plasmid number 67638 [ ]) was used to introduce a double-strand break at the ADE2 locus, 57 bp into the coding sequence. pML104 was isolated from dam-INV110 Escherichia coli (ThermoFisher number C717103) and digested with SwaI (NEB number R0604) and BclI (NEB number R0160). ..

    Expressing:

    Article Title: Decoding the chromatin proteome of a single genomic locus by DNA sequencing
    Article Snippet: .. Preparation of the Cas9/gRNA vector The Cas9 and gRNA expression vector pML104 (Addgene plasmid number 67638 [ ]) was used to introduce a double-strand break at the ADE2 locus, 57 bp into the coding sequence. pML104 was isolated from dam-INV110 Escherichia coli (ThermoFisher number C717103) and digested with SwaI (NEB number R0604) and BclI (NEB number R0160). ..

    Introduce:

    Article Title: Decoding the chromatin proteome of a single genomic locus by DNA sequencing
    Article Snippet: .. Preparation of the Cas9/gRNA vector The Cas9 and gRNA expression vector pML104 (Addgene plasmid number 67638 [ ]) was used to introduce a double-strand break at the ADE2 locus, 57 bp into the coding sequence. pML104 was isolated from dam-INV110 Escherichia coli (ThermoFisher number C717103) and digested with SwaI (NEB number R0604) and BclI (NEB number R0160). ..

    Sequencing:

    Article Title: Decoding the chromatin proteome of a single genomic locus by DNA sequencing
    Article Snippet: .. Preparation of the Cas9/gRNA vector The Cas9 and gRNA expression vector pML104 (Addgene plasmid number 67638 [ ]) was used to introduce a double-strand break at the ADE2 locus, 57 bp into the coding sequence. pML104 was isolated from dam-INV110 Escherichia coli (ThermoFisher number C717103) and digested with SwaI (NEB number R0604) and BclI (NEB number R0160). ..

    Isolation:

    Article Title: Decoding the chromatin proteome of a single genomic locus by DNA sequencing
    Article Snippet: .. Preparation of the Cas9/gRNA vector The Cas9 and gRNA expression vector pML104 (Addgene plasmid number 67638 [ ]) was used to introduce a double-strand break at the ADE2 locus, 57 bp into the coding sequence. pML104 was isolated from dam-INV110 Escherichia coli (ThermoFisher number C717103) and digested with SwaI (NEB number R0604) and BclI (NEB number R0160). ..

    Southern Blot:

    Article Title: Identification of Rv3852 as an Agrimophol-Binding Protein in Mycobacterium tuberculosis
    Article Snippet: .. For Southern blot analysis, genomic DNA from wild type Mtb and hygromycin resistant Mtb were digested by BclI (New England BioLabs) at 50°C overnight, run on the 1% agarose gel, transferred to the membrane (Amersham) and blotted by a designed probe. ..

    Agarose Gel Electrophoresis:

    Article Title: Identification of Rv3852 as an Agrimophol-Binding Protein in Mycobacterium tuberculosis
    Article Snippet: .. For Southern blot analysis, genomic DNA from wild type Mtb and hygromycin resistant Mtb were digested by BclI (New England BioLabs) at 50°C overnight, run on the 1% agarose gel, transferred to the membrane (Amersham) and blotted by a designed probe. ..

    Incubation:

    Article Title: New Vectors for Simple and Streamlined CRISPR-Cas9 Genome Editing in Saccharomyces cerevisiae
    Article Snippet: .. BclI (New England BioLabs) was subsequently added to the digestion reaction and incubated for a minimum of two hours at 50°C. ..

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  • 94
    New England Biolabs bcli
    Integration does not occur in host telomeres in the absence of viral TMRs. (A) Schematic representation and corresponding PFGE and Southern blot analysis of LCL DNA digested with <t>SfiI.</t> Fragment sizes generated by SfiI digestion of integrated and nonintegrated MDV genomes are depicted and sizes are given. The size of the linear MDV genome observed during lytic replication is indicated by an arrow. Results are representative of three independent Southern blot analyses. (B) Southern blotting of DNA of LCL derived from animals infected with vTE1 and digested with <t>BclI.</t> Potential intragenomic and telomeric integration sites are indicated. Results are representative of three independent Southern blot analyses. (C) Quantification of MDV copies in tumor cells. Results are shown as mean herpesvirus genome copies detected by the TR L probe relative to B2M in three independent experiments. The data are shown relative to LCL CU482 derived from a vRB-1B–infected chicken with standard deviations (error bars).
    Bcli, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bcli/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bcli - by Bioz Stars, 2021-08
    94/100 stars
      Buy from Supplier

    Image Search Results


    Integration does not occur in host telomeres in the absence of viral TMRs. (A) Schematic representation and corresponding PFGE and Southern blot analysis of LCL DNA digested with SfiI. Fragment sizes generated by SfiI digestion of integrated and nonintegrated MDV genomes are depicted and sizes are given. The size of the linear MDV genome observed during lytic replication is indicated by an arrow. Results are representative of three independent Southern blot analyses. (B) Southern blotting of DNA of LCL derived from animals infected with vTE1 and digested with BclI. Potential intragenomic and telomeric integration sites are indicated. Results are representative of three independent Southern blot analyses. (C) Quantification of MDV copies in tumor cells. Results are shown as mean herpesvirus genome copies detected by the TR L probe relative to B2M in three independent experiments. The data are shown relative to LCL CU482 derived from a vRB-1B–infected chicken with standard deviations (error bars).

    Journal: The Journal of Experimental Medicine

    Article Title: Herpesvirus telomeric repeats facilitate genomic integration into host telomeres and mobilization of viral DNA during reactivation

    doi: 10.1084/jem.20101402

    Figure Lengend Snippet: Integration does not occur in host telomeres in the absence of viral TMRs. (A) Schematic representation and corresponding PFGE and Southern blot analysis of LCL DNA digested with SfiI. Fragment sizes generated by SfiI digestion of integrated and nonintegrated MDV genomes are depicted and sizes are given. The size of the linear MDV genome observed during lytic replication is indicated by an arrow. Results are representative of three independent Southern blot analyses. (B) Southern blotting of DNA of LCL derived from animals infected with vTE1 and digested with BclI. Potential intragenomic and telomeric integration sites are indicated. Results are representative of three independent Southern blot analyses. (C) Quantification of MDV copies in tumor cells. Results are shown as mean herpesvirus genome copies detected by the TR L probe relative to B2M in three independent experiments. The data are shown relative to LCL CU482 derived from a vRB-1B–infected chicken with standard deviations (error bars).

    Article Snippet: 1 × 107 /ml LCLs were embedded in 1% agarose and digested at 50°C for 48 h hours in lysis buffer (0.5 M EDTA and 1% wt/vol N -laurylsarcosine) containing 1 mg/ml proteinase K. Proteinase K was inactivated with 0.01 mM phenylmethanesulfonyl fluoride, and agarose plugs were digested with either SfiI or BclI (New England Biolabs) overnight according to the manufacturer’s instructions.

    Techniques: Southern Blot, Generated, Derivative Assay, Infection

    Experimental strategy for cloning user-designed guide sequence into sgRNA expression cassette in pML104 or pML107. The BclI site is located at the 3’ end of the pSNR52 promoter, which is used to drive sgRNA expression in yeast. The SwaI site is located in the structural portion of the sgRNA. SwaI and BclI ).

    Journal: Current protocols in molecular biology

    Article Title: Simple CRISPR-Cas9 Genome Editing in S. cerevisiae

    doi: 10.1002/cpmb.110

    Figure Lengend Snippet: Experimental strategy for cloning user-designed guide sequence into sgRNA expression cassette in pML104 or pML107. The BclI site is located at the 3’ end of the pSNR52 promoter, which is used to drive sgRNA expression in yeast. The SwaI site is located in the structural portion of the sgRNA. SwaI and BclI ).

    Article Snippet: Restriction enzymes SwaI (NEB #R0604S) and BclI (NEB #R0160S).

    Techniques: Clone Assay, Sequencing, Expressing

    Plasmid maps of the sgRNA/Cas9 expression vectors pML104 ( URA3 marker) and pML107 ( LEU2 marker). Both plasmids are yeast/ E. coli shuttle vectors containing an ampicillin resistance (AmpR) marker, and a yeast 2 micron (2μ) origin of replication. Both vectors contain a Cas9 expression cassette and an sgRNA expression cassette, which contains unique BclI and SwaI restriction sites, to facilitate directional cloning of the user-designed guide sequence into the sgRNA expression cassette.

    Journal: Current protocols in molecular biology

    Article Title: Simple CRISPR-Cas9 Genome Editing in S. cerevisiae

    doi: 10.1002/cpmb.110

    Figure Lengend Snippet: Plasmid maps of the sgRNA/Cas9 expression vectors pML104 ( URA3 marker) and pML107 ( LEU2 marker). Both plasmids are yeast/ E. coli shuttle vectors containing an ampicillin resistance (AmpR) marker, and a yeast 2 micron (2μ) origin of replication. Both vectors contain a Cas9 expression cassette and an sgRNA expression cassette, which contains unique BclI and SwaI restriction sites, to facilitate directional cloning of the user-designed guide sequence into the sgRNA expression cassette.

    Article Snippet: Restriction enzymes SwaI (NEB #R0604S) and BclI (NEB #R0160S).

    Techniques: Plasmid Preparation, Expressing, Marker, Clone Assay, Sequencing