avaii  (New England Biolabs)


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    • 93
    Name:
    AvaII
    Description:
    AvaII 10 000 units
    Catalog Number:
    r0153l
    Price:
    269
    Size:
    10 000 units
    Category:
    Restriction Enzymes
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    Structured Review

    New England Biolabs avaii
    AvaII
    AvaII 10 000 units
    https://www.bioz.com/result/avaii/product/New England Biolabs
    Average 93 stars, based on 16464 article reviews
    Price from $9.99 to $1999.99
    avaii - by Bioz Stars, 2021-02
    93/100 stars

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    Images

    1) Product Images from "Control of alternative splicing by forskolin through hnRNP K during neuronal differentiation"

    Article Title: Control of alternative splicing by forskolin through hnRNP K during neuronal differentiation

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gks504

    Isolation of a KARRE from the upstream 3′ splice site of the exon 5a of Snap25 gene based on forskolin reduction of 5a/5b ratios in PC12 cells during neuronal differentiation. ( A ) Forskolin-induced neuronal differentiation of PC12 cells. Shown are representative images of PC12 cells without or with forskolin addition for 18 h. ( B ) Diagram of the alternative splicing of the exons 5a and 5b of the Snap25 gene. Arrows: sites of restriction enzymes NdeI (5a) and AvaII (5b) used to digest the PCR products. Arrowheads: PCR primers. ( C ) RT-PCR products from untreated (−) and treated PC12 cells. ETOH: ethanol, vehicle for forskolin and Dex (dexamethasone); H89: a PKA-specific inhibitor; NC: PCR negative control; M: molecular size marker. ( D) Diagram of the deletion/replacement mutants of Snap25 splicing reporters and their exon inclusion levels in the presence of PKAm or active PKA. Asterisks: indicating P value levels in one tail, paired Student’s t -test (*** P
    Figure Legend Snippet: Isolation of a KARRE from the upstream 3′ splice site of the exon 5a of Snap25 gene based on forskolin reduction of 5a/5b ratios in PC12 cells during neuronal differentiation. ( A ) Forskolin-induced neuronal differentiation of PC12 cells. Shown are representative images of PC12 cells without or with forskolin addition for 18 h. ( B ) Diagram of the alternative splicing of the exons 5a and 5b of the Snap25 gene. Arrows: sites of restriction enzymes NdeI (5a) and AvaII (5b) used to digest the PCR products. Arrowheads: PCR primers. ( C ) RT-PCR products from untreated (−) and treated PC12 cells. ETOH: ethanol, vehicle for forskolin and Dex (dexamethasone); H89: a PKA-specific inhibitor; NC: PCR negative control; M: molecular size marker. ( D) Diagram of the deletion/replacement mutants of Snap25 splicing reporters and their exon inclusion levels in the presence of PKAm or active PKA. Asterisks: indicating P value levels in one tail, paired Student’s t -test (*** P

    Techniques Used: Isolation, Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction, Negative Control, Marker

    2) Product Images from "Identification of Infectious Agents in Onychomycoses by PCR-Terminal Restriction Fragment Length Polymorphism"

    Article Title: Identification of Infectious Agents in Onychomycoses by PCR-Terminal Restriction Fragment Length Polymorphism

    Journal: Journal of Clinical Microbiology

    doi: 10.1128/JCM.05164-11

    Overview of the PCR-TRFLP assay developed for fungal identification in onychomycosis. First, fungal DNA was extracted from nail samples. Then, 28S rDNA was amplified using a labeled forward primer. A single-step digestion of PCR amplicons with AvaI, AvaII,
    Figure Legend Snippet: Overview of the PCR-TRFLP assay developed for fungal identification in onychomycosis. First, fungal DNA was extracted from nail samples. Then, 28S rDNA was amplified using a labeled forward primer. A single-step digestion of PCR amplicons with AvaI, AvaII,

    Techniques Used: Polymerase Chain Reaction, Terminal Restriction Fragment Length Polymorphism, Amplification, Labeling

    Related Articles

    Clone Assay:

    Article Title: A dendritic cell receptor-targeted chimeric immunotherapeutic protein (C-HBV) for the treatment of chronic hepatitis B.
    Article Snippet: .. After digestion with Ava II (New England Biolab, R0153S), and Rsr II (New England Biolabs, R051S), the fragment was cloned into Rsr II digested pFastBacHTa, which places the gp64 signal sequence just upstream of the 6xHis tag to generate pFastBacHTa-gp64. .. The S1/S2/Core/TBD insert in pUC57was isolated by digestion with Sal I (New England Biolabs, R3138S) and Hind III (New England Biolabs, R0104S) and cloned into Sal I and Hind III digested pFastBacHTa-gp64.

    Article Title: Endotoxin-tolerant Mice Have Mutations in Toll-like Receptor 4 (Tlr4)
    Article Snippet: .. DNA samples (5 μg) from 10 BAC, 2 P1, and 58 cosmid clones derived from YAC 89A3 were digested with AluI or a combination of HaeIII, AvaII, and HincII using conditions provided by the supplier ( New England Biolabs ). .. Individual DNA digests were purified using standard protocols , ligated into EcoRV site of pBluescript II KS+ (Stratagene), and transformed to Escherichia coli strain XL1-MRF′.

    BAC Assay:

    Article Title: Endotoxin-tolerant Mice Have Mutations in Toll-like Receptor 4 (Tlr4)
    Article Snippet: .. DNA samples (5 μg) from 10 BAC, 2 P1, and 58 cosmid clones derived from YAC 89A3 were digested with AluI or a combination of HaeIII, AvaII, and HincII using conditions provided by the supplier ( New England Biolabs ). .. Individual DNA digests were purified using standard protocols , ligated into EcoRV site of pBluescript II KS+ (Stratagene), and transformed to Escherichia coli strain XL1-MRF′.

    Mutagenesis:

    Article Title: Targetable cellular signaling events mediate vascular pathology in vascular Ehlers-Danlos syndrome
    Article Snippet: .. The G209S mutation leads to the loss of an AvaII cut site and the G938D mutation leads to the gain of a BamHI cut site (AvaII R0153L; BamHI-HF R3136S, New England Biolabs). ..

    Sequencing:

    Article Title: A dendritic cell receptor-targeted chimeric immunotherapeutic protein (C-HBV) for the treatment of chronic hepatitis B.
    Article Snippet: .. After digestion with Ava II (New England Biolab, R0153S), and Rsr II (New England Biolabs, R051S), the fragment was cloned into Rsr II digested pFastBacHTa, which places the gp64 signal sequence just upstream of the 6xHis tag to generate pFastBacHTa-gp64. .. The S1/S2/Core/TBD insert in pUC57was isolated by digestion with Sal I (New England Biolabs, R3138S) and Hind III (New England Biolabs, R0104S) and cloned into Sal I and Hind III digested pFastBacHTa-gp64.

    Generated:

    Article Title: The relationship between transplacental O2 diffusion and placental expression of PlGF, VEGF and their receptors in a placental insufficiency model of fetal growth restriction
    Article Snippet: .. Briefly, VEGFR-1 and VEGFR-2 cDNAs were digested with Tfil (New England BioLabs, Beverly, MA, USA), which when used in the RPA, generated protected fragments of 184 and 198 bp respectively, whilst actin was digested with AvaII (New England BioLabs) to yield a protected fragment of 141 bp. .. Antisense [α-32 P]CTP-radiolabelled cRNA probes (2 × 105 c.p.m.) were synthesized by in vitro transcription using either T3 or T7 RNA polymerases (Ambion, Austin, TX, USA) in the presence of unlabelled NTPs.

    Polyacrylamide Gel Electrophoresis:

    Article Title: Control of alternative splicing by forskolin through hnRNP K during neuronal differentiation
    Article Snippet: .. Some cultures were pretreated with 10 µM of H89 (N -[2-(p-bromocinnamylamino)ethyl]-5-isoquinolinesulfonamidedihydrochloride, Sigma, #B1427) for 10 min. To differentiate products of Snap25 endogenous mRNA transcripts from exons 5a and 5b, we digested 1.0 µl of 32 P-labelled-PCR products with AvaII and NdeI restriction enzymes in buffer 4 (New England Biolabs) in a 10 -µl reaction at 37°C for 1 h and run in 6% denaturing polyacrylamide gel electrophoresis (PAGE) gel. .. For minigene splicing reporters, 24 cycles of PCR were carried out and products resolved in 3% agarose gels stained with ethidium bromide.

    Polymerase Chain Reaction:

    Article Title: High prevalence of pfdhfr-pfdhps triple mutations associated with anti-malarial drugs resistance in Plasmodium falciparum isolates seven years after the adoption of sulfadoxine-pyrimethamine in combination with artesunate as first-line treatment in Iran.
    Article Snippet: .. The spread of anti-malarial drug resistance will challenge any malaria control and elimination strategies, and routine monitoring of resistance-associated molecular markers of commonly used anti-malarial drugs is very important. .. The spread of anti-malarial drug resistance will challenge any malaria control and elimination strategies, and routine monitoring of resistance-associated molecular markers of commonly used anti-malarial drugs is very important.

    Article Title: Identification of Infectious Agents in Onychomycoses by PCR-Terminal Restriction Fragment Length Polymorphism
    Article Snippet: .. Restriction enzyme digestions were performed at 37°C for 60 min. Twenty microliters of PCR product; 1 μl of AvaI, 1 μl of AvaII, and 1 μl of StuI restriction endonucleases (New England BioLabs, Ipswich, MA); and 5 μl of 10× reaction buffer (NEBuffer 4) were mixed with deionized water to give a total reaction volume of 50 μl. .. Restriction fragments were subsequently purified using a High Pure PCR Purification kit (Roche Diagnostics, Basel, Switzerland).

    Antiviral Assay:

    Article Title: A dendritic cell receptor-targeted chimeric immunotherapeutic protein (C-HBV) for the treatment of chronic hepatitis B.
    Article Snippet: .. After digestion with Ava II (New England Biolab, R0153S), and Rsr II (New England Biolabs, R051S), the fragment was cloned into Rsr II digested pFastBacHTa, which places the gp64 signal sequence just upstream of the 6xHis tag to generate pFastBacHTa-gp64. .. The S1/S2/Core/TBD insert in pUC57was isolated by digestion with Sal I (New England Biolabs, R3138S) and Hind III (New England Biolabs, R0104S) and cloned into Sal I and Hind III digested pFastBacHTa-gp64.

    Recombinase Polymerase Amplification:

    Article Title: The relationship between transplacental O2 diffusion and placental expression of PlGF, VEGF and their receptors in a placental insufficiency model of fetal growth restriction
    Article Snippet: .. Briefly, VEGFR-1 and VEGFR-2 cDNAs were digested with Tfil (New England BioLabs, Beverly, MA, USA), which when used in the RPA, generated protected fragments of 184 and 198 bp respectively, whilst actin was digested with AvaII (New England BioLabs) to yield a protected fragment of 141 bp. .. Antisense [α-32 P]CTP-radiolabelled cRNA probes (2 × 105 c.p.m.) were synthesized by in vitro transcription using either T3 or T7 RNA polymerases (Ambion, Austin, TX, USA) in the presence of unlabelled NTPs.

    Derivative Assay:

    Article Title: Endotoxin-tolerant Mice Have Mutations in Toll-like Receptor 4 (Tlr4)
    Article Snippet: .. DNA samples (5 μg) from 10 BAC, 2 P1, and 58 cosmid clones derived from YAC 89A3 were digested with AluI or a combination of HaeIII, AvaII, and HincII using conditions provided by the supplier ( New England Biolabs ). .. Individual DNA digests were purified using standard protocols , ligated into EcoRV site of pBluescript II KS+ (Stratagene), and transformed to Escherichia coli strain XL1-MRF′.

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    • avaii  (New England Biolabs)
    93
    New England Biolabs avaii
    Isolation of a KARRE from the upstream 3′ splice site of the exon <t>5a</t> of Snap25 gene based on forskolin reduction of 5a/5b ratios in PC12 cells during neuronal differentiation. ( A ) Forskolin-induced neuronal differentiation of PC12 cells. Shown are representative images of PC12 cells without or with forskolin addition for 18 h. ( B ) Diagram of the alternative splicing of the exons 5a and 5b of the Snap25 gene. Arrows: sites of restriction enzymes NdeI (5a) and <t>AvaII</t> (5b) used to digest the PCR products. Arrowheads: PCR primers. ( C ) RT-PCR products from untreated (−) and treated PC12 cells. ETOH: ethanol, vehicle for forskolin and Dex (dexamethasone); H89: a PKA-specific inhibitor; NC: PCR negative control; M: molecular size marker. ( D) Diagram of the deletion/replacement mutants of Snap25 splicing reporters and their exon inclusion levels in the presence of PKAm or active PKA. Asterisks: indicating P value levels in one tail, paired Student’s t -test (*** P
    Avaii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/avaii/product/New England Biolabs
    Average 93 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    avaii - by Bioz Stars, 2021-02
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    Isolation of a KARRE from the upstream 3′ splice site of the exon 5a of Snap25 gene based on forskolin reduction of 5a/5b ratios in PC12 cells during neuronal differentiation. ( A ) Forskolin-induced neuronal differentiation of PC12 cells. Shown are representative images of PC12 cells without or with forskolin addition for 18 h. ( B ) Diagram of the alternative splicing of the exons 5a and 5b of the Snap25 gene. Arrows: sites of restriction enzymes NdeI (5a) and AvaII (5b) used to digest the PCR products. Arrowheads: PCR primers. ( C ) RT-PCR products from untreated (−) and treated PC12 cells. ETOH: ethanol, vehicle for forskolin and Dex (dexamethasone); H89: a PKA-specific inhibitor; NC: PCR negative control; M: molecular size marker. ( D) Diagram of the deletion/replacement mutants of Snap25 splicing reporters and their exon inclusion levels in the presence of PKAm or active PKA. Asterisks: indicating P value levels in one tail, paired Student’s t -test (*** P

    Journal: Nucleic Acids Research

    Article Title: Control of alternative splicing by forskolin through hnRNP K during neuronal differentiation

    doi: 10.1093/nar/gks504

    Figure Lengend Snippet: Isolation of a KARRE from the upstream 3′ splice site of the exon 5a of Snap25 gene based on forskolin reduction of 5a/5b ratios in PC12 cells during neuronal differentiation. ( A ) Forskolin-induced neuronal differentiation of PC12 cells. Shown are representative images of PC12 cells without or with forskolin addition for 18 h. ( B ) Diagram of the alternative splicing of the exons 5a and 5b of the Snap25 gene. Arrows: sites of restriction enzymes NdeI (5a) and AvaII (5b) used to digest the PCR products. Arrowheads: PCR primers. ( C ) RT-PCR products from untreated (−) and treated PC12 cells. ETOH: ethanol, vehicle for forskolin and Dex (dexamethasone); H89: a PKA-specific inhibitor; NC: PCR negative control; M: molecular size marker. ( D) Diagram of the deletion/replacement mutants of Snap25 splicing reporters and their exon inclusion levels in the presence of PKAm or active PKA. Asterisks: indicating P value levels in one tail, paired Student’s t -test (*** P

    Article Snippet: Some cultures were pretreated with 10 µM of H89 (N -[2-(p-bromocinnamylamino)ethyl]-5-isoquinolinesulfonamidedihydrochloride, Sigma, #B1427) for 10 min. To differentiate products of Snap25 endogenous mRNA transcripts from exons 5a and 5b, we digested 1.0 µl of 32 P-labelled-PCR products with AvaII and NdeI restriction enzymes in buffer 4 (New England Biolabs) in a 10 -µl reaction at 37°C for 1 h and run in 6% denaturing polyacrylamide gel electrophoresis (PAGE) gel.

    Techniques: Isolation, Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction, Negative Control, Marker

    Overview of the PCR-TRFLP assay developed for fungal identification in onychomycosis. First, fungal DNA was extracted from nail samples. Then, 28S rDNA was amplified using a labeled forward primer. A single-step digestion of PCR amplicons with AvaI, AvaII,

    Journal: Journal of Clinical Microbiology

    Article Title: Identification of Infectious Agents in Onychomycoses by PCR-Terminal Restriction Fragment Length Polymorphism

    doi: 10.1128/JCM.05164-11

    Figure Lengend Snippet: Overview of the PCR-TRFLP assay developed for fungal identification in onychomycosis. First, fungal DNA was extracted from nail samples. Then, 28S rDNA was amplified using a labeled forward primer. A single-step digestion of PCR amplicons with AvaI, AvaII,

    Article Snippet: Restriction enzyme digestions were performed at 37°C for 60 min. Twenty microliters of PCR product; 1 μl of AvaI, 1 μl of AvaII, and 1 μl of StuI restriction endonucleases (New England BioLabs, Ipswich, MA); and 5 μl of 10× reaction buffer (NEBuffer 4) were mixed with deionized water to give a total reaction volume of 50 μl.

    Techniques: Polymerase Chain Reaction, Terminal Restriction Fragment Length Polymorphism, Amplification, Labeling