avaii (New England Biolabs)


Name:
AvaII
Description:
AvaII 10 000 units
Catalog Number:
r0153l
Price:
269
Size:
10 000 units
Category:
Restriction Enzymes
|
Buy from Supplier |
Structured Review

AvaII 10 000 units
https://www.bioz.com/result/avaii/product/New England Biolabs
Average 93 stars, based on 16464 article reviews
Price from $9.99 to $1999.99
Related Products / Commonly Used Together
Images
1) Product Images from "Control of alternative splicing by forskolin through hnRNP K during neuronal differentiation"
Article Title: Control of alternative splicing by forskolin through hnRNP K during neuronal differentiation
Journal: Nucleic Acids Research
doi: 10.1093/nar/gks504

Figure Legend Snippet: Isolation of a KARRE from the upstream 3′ splice site of the exon 5a of Snap25 gene based on forskolin reduction of 5a/5b ratios in PC12 cells during neuronal differentiation. ( A ) Forskolin-induced neuronal differentiation of PC12 cells. Shown are representative images of PC12 cells without or with forskolin addition for 18 h. ( B ) Diagram of the alternative splicing of the exons 5a and 5b of the Snap25 gene. Arrows: sites of restriction enzymes NdeI (5a) and AvaII (5b) used to digest the PCR products. Arrowheads: PCR primers. ( C ) RT-PCR products from untreated (−) and treated PC12 cells. ETOH: ethanol, vehicle for forskolin and Dex (dexamethasone); H89: a PKA-specific inhibitor; NC: PCR negative control; M: molecular size marker. ( D) Diagram of the deletion/replacement mutants of Snap25 splicing reporters and their exon inclusion levels in the presence of PKAm or active PKA. Asterisks: indicating P value levels in one tail, paired Student’s t -test (*** P
Techniques Used: Isolation, Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction, Negative Control, Marker
2) Product Images from "Identification of Infectious Agents in Onychomycoses by PCR-Terminal Restriction Fragment Length Polymorphism"
Article Title: Identification of Infectious Agents in Onychomycoses by PCR-Terminal Restriction Fragment Length Polymorphism
Journal: Journal of Clinical Microbiology
doi: 10.1128/JCM.05164-11

Figure Legend Snippet: Overview of the PCR-TRFLP assay developed for fungal identification in onychomycosis. First, fungal DNA was extracted from nail samples. Then, 28S rDNA was amplified using a labeled forward primer. A single-step digestion of PCR amplicons with AvaI, AvaII,
Techniques Used: Polymerase Chain Reaction, Terminal Restriction Fragment Length Polymorphism, Amplification, Labeling
Related Articles
Clone Assay:Article Title: A dendritic cell receptor-targeted chimeric immunotherapeutic protein (C-HBV) for the treatment of chronic hepatitis B. Article Snippet: .. After digestion with Article Title: Endotoxin-tolerant Mice Have Mutations in Toll-like Receptor 4 (Tlr4) Article Snippet: .. DNA samples (5 μg) from 10 BAC, 2 P1, and 58 cosmid clones derived from YAC 89A3 were digested with AluI or a combination of HaeIII, BAC Assay:Article Title: Endotoxin-tolerant Mice Have Mutations in Toll-like Receptor 4 (Tlr4) Article Snippet: .. DNA samples (5 μg) from 10 BAC, 2 P1, and 58 cosmid clones derived from YAC 89A3 were digested with AluI or a combination of HaeIII, Mutagenesis:Article Title: Targetable cellular signaling events mediate vascular pathology in vascular Ehlers-Danlos syndrome Article Snippet: .. The G209S mutation leads to the loss of an AvaII cut site and the G938D mutation leads to the gain of a Sequencing:Article Title: A dendritic cell receptor-targeted chimeric immunotherapeutic protein (C-HBV) for the treatment of chronic hepatitis B. Article Snippet: .. After digestion with Generated:Article Title: The relationship between transplacental O2 diffusion and placental expression of PlGF, VEGF and their receptors in a placental insufficiency model of fetal growth restriction Article Snippet: .. Briefly, VEGFR-1 and VEGFR-2 cDNAs were digested with Tfil (New England BioLabs, Beverly, MA, USA), which when used in the RPA, generated protected fragments of 184 and 198 bp respectively, whilst actin was digested with Polyacrylamide Gel Electrophoresis:Article Title: Control of alternative splicing by forskolin through hnRNP K during neuronal differentiation Article Snippet: .. Some cultures were pretreated with 10 µM of H89 (N -[2-(p-bromocinnamylamino)ethyl]-5-isoquinolinesulfonamidedihydrochloride, Sigma, #B1427) for 10 min. To differentiate products of Snap25 endogenous mRNA transcripts from exons 5a and 5b, we digested 1.0 µl of 32 P-labelled-PCR products with Polymerase Chain Reaction:Article Title: High prevalence of pfdhfr-pfdhps triple mutations associated with anti-malarial drugs resistance in Plasmodium falciparum isolates seven years after the adoption of sulfadoxine-pyrimethamine in combination with artesunate as first-line treatment in Iran. Article Snippet: .. The spread of anti-malarial drug resistance will challenge any malaria control Article Title: Identification of Infectious Agents in Onychomycoses by PCR-Terminal Restriction Fragment Length Polymorphism Article Snippet: .. Restriction enzyme digestions were performed at 37°C for 60 min. Twenty microliters of PCR product; 1 μl of AvaI, 1 μl of Antiviral Assay:Article Title: A dendritic cell receptor-targeted chimeric immunotherapeutic protein (C-HBV) for the treatment of chronic hepatitis B. Article Snippet: .. After digestion with Recombinase Polymerase Amplification:Article Title: The relationship between transplacental O2 diffusion and placental expression of PlGF, VEGF and their receptors in a placental insufficiency model of fetal growth restriction Article Snippet: .. Briefly, VEGFR-1 and VEGFR-2 cDNAs were digested with Tfil (New England BioLabs, Beverly, MA, USA), which when used in the RPA, generated protected fragments of 184 and 198 bp respectively, whilst actin was digested with Derivative Assay:Article Title: Endotoxin-tolerant Mice Have Mutations in Toll-like Receptor 4 (Tlr4) Article Snippet: .. DNA samples (5 μg) from 10 BAC, 2 P1, and 58 cosmid clones derived from YAC 89A3 were digested with AluI or a combination of HaeIII, |