avaii (New England Biolabs)

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Name:

AvaII

Description:

AvaII 10 000 units

Catalog Number:

R0153L

Price:

269

Category:

Restriction Enzymes

Size:

10 000 units

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New England Biolabs avaii
AvaII

AvaII 10 000 units
https://www.bioz.com/result/avaii/product/New England Biolabs
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99

avaii - by Bioz Stars, 2021-05

93/100 stars

Images

1) Product Images from "Identification of Infectious Agents in Onychomycoses by PCR-Terminal Restriction Fragment Length Polymorphism"

Article Title: Identification of Infectious Agents in Onychomycoses by PCR-Terminal Restriction Fragment Length Polymorphism

Journal: Journal of Clinical Microbiology

doi: 10.1128/JCM.05164-11

Overview of the PCR-TRFLP assay developed for fungal identification in onychomycosis. First, fungal DNA was extracted from nail samples. Then, 28S rDNA was amplified using a labeled forward primer. A single-step digestion of PCR amplicons with AvaI, AvaII,

Figure Legend Snippet: Overview of the PCR-TRFLP assay developed for fungal identification in onychomycosis. First, fungal DNA was extracted from nail samples. Then, 28S rDNA was amplified using a labeled forward primer. A single-step digestion of PCR amplicons with AvaI, AvaII,

Techniques Used: Polymerase Chain Reaction, Terminal Restriction Fragment Length Polymorphism, Amplification, Labeling

2) Product Images from "Control of alternative splicing by forskolin through hnRNP K during neuronal differentiation"

Article Title: Control of alternative splicing by forskolin through hnRNP K during neuronal differentiation

Journal: Nucleic Acids Research

doi: 10.1093/nar/gks504

Isolation of a KARRE from the upstream 3′ splice site of the exon 5a of Snap25 gene based on forskolin reduction of 5a/5b ratios in PC12 cells during neuronal differentiation. ( A ) Forskolin-induced neuronal differentiation of PC12 cells. Shown are representative images of PC12 cells without or with forskolin addition for 18 h. ( B ) Diagram of the alternative splicing of the exons 5a and 5b of the Snap25 gene. Arrows: sites of restriction enzymes NdeI (5a) and AvaII (5b) used to digest the PCR products. Arrowheads: PCR primers. ( C ) RT-PCR products from untreated (−) and treated PC12 cells. ETOH: ethanol, vehicle for forskolin and Dex (dexamethasone); H89: a PKA-specific inhibitor; NC: PCR negative control; M: molecular size marker. ( D) Diagram of the deletion/replacement mutants of Snap25 splicing reporters and their exon inclusion levels in the presence of PKAm or active PKA. Asterisks: indicating P value levels in one tail, paired Student’s t -test (*** P

Figure Legend Snippet: Isolation of a KARRE from the upstream 3′ splice site of the exon 5a of Snap25 gene based on forskolin reduction of 5a/5b ratios in PC12 cells during neuronal differentiation. ( A ) Forskolin-induced neuronal differentiation of PC12 cells. Shown are representative images of PC12 cells without or with forskolin addition for 18 h. ( B ) Diagram of the alternative splicing of the exons 5a and 5b of the Snap25 gene. Arrows: sites of restriction enzymes NdeI (5a) and AvaII (5b) used to digest the PCR products. Arrowheads: PCR primers. ( C ) RT-PCR products from untreated (−) and treated PC12 cells. ETOH: ethanol, vehicle for forskolin and Dex (dexamethasone); H89: a PKA-specific inhibitor; NC: PCR negative control; M: molecular size marker. ( D) Diagram of the deletion/replacement mutants of Snap25 splicing reporters and their exon inclusion levels in the presence of PKAm or active PKA. Asterisks: indicating P value levels in one tail, paired Student’s t -test (*** P

Techniques Used: Isolation, Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction, Negative Control, Marker

Polymerase Chain Reaction:

Article Title: Identification of Infectious Agents in Onychomycoses by PCR-Terminal Restriction Fragment Length Polymorphism
Article Snippet: The reaction mixture was incubated for 1 min at 94°C; subjected to 30 cycles of 0.5 min at 94°C, 0.5 min at 55°C, and 0.5 min at 72°C; and finally incubated for 10 min at 72°C on an ABI 2720 thermocycler (Applied Biosystems, Inc., Carlsbad, CA). .. Restriction enzyme digestions were performed at 37°C for 60 min. Twenty microliters of PCR product; 1 μl of AvaI, 1 μl of AvaII, and 1 μl of StuI restriction endonucleases (New England BioLabs, Ipswich, MA); and 5 μl of 10× reaction buffer (NEBuffer 4) were mixed with deionized water to give a total reaction volume of 50 μl. .. Restriction fragments were subsequently purified using a High Pure PCR Purification kit (Roche Diagnostics, Basel, Switzerland).

Article Title: High prevalence of pfdhfr-pfdhps triple mutations associated with anti-malarial drugs resistance in Plasmodium falciparum isolates seven years after the adoption of sulfadoxine-pyrimethamine in combination with artesunate as first-line treatment in Iran.
Article Snippet: The spread of anti-malarial drug resistance will challenge any malaria control and elimination strategies, and routine monitoring of resistance-associated molecular markers of commonly used anti-malarial drugs is very important. .. The spread of anti-malarial drug resistance will challenge any malaria control and elimination strategies, and routine monitoring of resistance-associated molecular markers of commonly used anti-malarial drugs is very important. .. The spread of anti-malarial drug resistance will challenge any malaria control and elimination strategies, and routine monitoring of resistance-associated molecular markers of commonly used anti-malarial drugs is very important.

Incubation:

Article Title: Regulation of Human RNA Polymerase III Transcription by DNMT1 and DNMT3a DNA Methyltransferases *
Article Snippet: The methylation status of the CpG start site at the endogenous U6-1 gene was analyzed using ∼100 ng of genomic DNA harvested from HeLa or MCF7 cells. .. Genomic DNA was incubated with TaaI (Fermentas), HpyCH4III, or AvaII (New England Biolabs) or with no restriction enzyme overnight at either 37 °C (HpyCH4III and AvaII) or 65 °C (TaaI). .. Digested DNA was recovered by phenol extraction and ethanol precipitation followed by PCR analysis using primers spanning the U6 start site (U6-FOR, 5′-AAG TAT TTC GAT TTC TTG GC-3′; U6-REV, 5′-AAT ATG GAA CGC TTC ACG-3′) and GAPDH exon 2 (GAPDH-FOR, 5′-AGG TCA TCC CTG AGC TGA AC-3′; GAPDH-REV, 5′-GCA ATG CCA GCC CCA GCG TC-3′).

Polyacrylamide Gel Electrophoresis:

Article Title: Control of alternative splicing by forskolin through hnRNP K during neuronal differentiation
Article Snippet: Overnight cultures of PC12 cells were treated with cpt-cAMP (8-(4-Chlorophenylthio)adenosine 3′,5′-cyclic monophosphate sodium salt, 100 µM), forskolin (10 µM), KCl (25 mM) or dexamethasone (100 µM) for 6 hours before RNA extraction. .. Some cultures were pretreated with 10 µM of H89 (N -[2-(p-bromocinnamylamino)ethyl]-5-isoquinolinesulfonamidedihydrochloride, Sigma, #B1427) for 10 min. To differentiate products of Snap25 endogenous mRNA transcripts from exons 5a and 5b, we digested 1.0 µl of 32 P-labelled-PCR products with AvaII and NdeI restriction enzymes in buffer 4 (New England Biolabs) in a 10 -µl reaction at 37°C for 1 h and run in 6% denaturing polyacrylamide gel electrophoresis (PAGE) gel. .. For minigene splicing reporters, 24 cycles of PCR were carried out and products resolved in 3% agarose gels stained with ethidium bromide.