pvui  (New England Biolabs)


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    Name:
    PvuI
    Description:
    PvuI 2 500 units
    Catalog Number:
    R0150L
    Price:
    298
    Category:
    Restriction Enzymes
    Size:
    2 500 units
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    Structured Review

    New England Biolabs pvui
    PvuI
    PvuI 2 500 units
    https://www.bioz.com/result/pvui/product/New England Biolabs
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pvui - by Bioz Stars, 2021-06
    98/100 stars

    Images

    1) Product Images from "Sequence-Specific DNA Detection at 10 fM by Electromechanical Signal Transduction"

    Article Title: Sequence-Specific DNA Detection at 10 fM by Electromechanical Signal Transduction

    Journal: Analytical Chemistry

    doi: 10.1021/ac5021408

    Schematic of DNA oligomer preparation. (a) Purified pET-21b plasmids were enzymatically digested by selected pairs of ScaI, PvuI, Pst I, BsaI, and EcoNI restriction enzymes, producing fragments of different lengths. The target DNA sequence complementary to the PNA probe is located beginning at plasmid position 4427 (orange band). Plasmid digestion by ScaI and PvuI produced a 110-base, target-containing fragment, T1. Plasmid digestion by PvuI and PstI produced a 125-base, target-free control fragment, C1. Other fragments were produced similarly: T2 (235 bases) using ScaI and PstI, T3 (419 bases) using ScaI and BsaI, T4 (1613 bases) using by PvuI and EcoNI), C2 (184 bases) using PstI and BsaI, C3 (309 bases) using PvuI and BsaI, and C4 (1503 bases) using ScaI and EcoNI. (b) Following digestion, the DNA was isolated by gel electrophoresis, extracted, and purified. (c) Purified double-stranded DNA was denatured and hybridized with bead–PNA probe conjugates. (d) DNA–PNA–bead mixture was injected into the micropipette for electrical detection.
    Figure Legend Snippet: Schematic of DNA oligomer preparation. (a) Purified pET-21b plasmids were enzymatically digested by selected pairs of ScaI, PvuI, Pst I, BsaI, and EcoNI restriction enzymes, producing fragments of different lengths. The target DNA sequence complementary to the PNA probe is located beginning at plasmid position 4427 (orange band). Plasmid digestion by ScaI and PvuI produced a 110-base, target-containing fragment, T1. Plasmid digestion by PvuI and PstI produced a 125-base, target-free control fragment, C1. Other fragments were produced similarly: T2 (235 bases) using ScaI and PstI, T3 (419 bases) using ScaI and BsaI, T4 (1613 bases) using by PvuI and EcoNI), C2 (184 bases) using PstI and BsaI, C3 (309 bases) using PvuI and BsaI, and C4 (1503 bases) using ScaI and EcoNI. (b) Following digestion, the DNA was isolated by gel electrophoresis, extracted, and purified. (c) Purified double-stranded DNA was denatured and hybridized with bead–PNA probe conjugates. (d) DNA–PNA–bead mixture was injected into the micropipette for electrical detection.

    Techniques Used: Purification, Positron Emission Tomography, Sequencing, Plasmid Preparation, Produced, Isolation, Nucleic Acid Electrophoresis, Injection

    Related Articles

    Purification:

    Article Title: The Fitness Landscapes of cis-Acting Binding Sites in Different Promoter and Environmental Contexts
    Article Snippet: Solexa sample prep and sequencing Conversion of pBR322 to pBR322 destroyed a Hind III site that overlapped the first two bases of the hexamer. .. Libraries that contained the wild type ( TTTAAT ) were digested with Hind III and Pvu I (NEB) for 2 hours at C. pBR322 libraries were digested with Cla I and Pvu I (NEB) for 2 hours at C. base pair fragments were gel purified for all four libraries using the QIAquick gel extraction kit. .. Excised fragments from all four promoter libraries, selected at a single tetracycline concentration, were mixed at equal concentration.

    Article Title: Rapid Identification of Rhodococcus equi by a PCR Assay Targeting the choE Gene
    Article Snippet: The sequences were analyzed by using the BLAST ( ) network service from the National Center for Biotechnology Information (Bethesda, Md.) and were aligned by use of the AlignX program (InforMax, Bethesda, Md.). .. For restriction fragment length polymorphism (RFLP) analysis, the choE amplicons were purified by use of the Gel Extraction Purification kit (Qiagen) and were subsequently digested with the following restriction enzymes: Ava I, Bam HI, Bgl I, Hin PI, Pvu I, Xho I, and Xmn I (New England Biolabs, Beverly, Mass.). .. The digestion products were resolved by electrophoresis on 1.2% agarose gels in Tris borate buffer and were visualized by ethidium bromide staining.

    Article Title: Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing
    Article Snippet: Transcribed crRNAs were subsequently purified with the miRNeasy Mini Kit (Qiagen). .. In vitro cleavage reactions consisted of 2.5 nM PvuI-linearized substrate plasmid, 300 nM crRNA, and 200 nM purified Cas12a protein in cleavage buffer (10 mM Hepes pH 7.5, 150 mM NaCl and 5 mM MgCl2), and were performed at 37 °C unless otherwise indicated. .. Plasmid substrates for temperature tolerance assays encoded the PAMDA site 2 spacer with a TTTA PAM (in and ).

    Gel Extraction:

    Article Title: The Fitness Landscapes of cis-Acting Binding Sites in Different Promoter and Environmental Contexts
    Article Snippet: Solexa sample prep and sequencing Conversion of pBR322 to pBR322 destroyed a Hind III site that overlapped the first two bases of the hexamer. .. Libraries that contained the wild type ( TTTAAT ) were digested with Hind III and Pvu I (NEB) for 2 hours at C. pBR322 libraries were digested with Cla I and Pvu I (NEB) for 2 hours at C. base pair fragments were gel purified for all four libraries using the QIAquick gel extraction kit. .. Excised fragments from all four promoter libraries, selected at a single tetracycline concentration, were mixed at equal concentration.

    Article Title: Rapid Identification of Rhodococcus equi by a PCR Assay Targeting the choE Gene
    Article Snippet: The sequences were analyzed by using the BLAST ( ) network service from the National Center for Biotechnology Information (Bethesda, Md.) and were aligned by use of the AlignX program (InforMax, Bethesda, Md.). .. For restriction fragment length polymorphism (RFLP) analysis, the choE amplicons were purified by use of the Gel Extraction Purification kit (Qiagen) and were subsequently digested with the following restriction enzymes: Ava I, Bam HI, Bgl I, Hin PI, Pvu I, Xho I, and Xmn I (New England Biolabs, Beverly, Mass.). .. The digestion products were resolved by electrophoresis on 1.2% agarose gels in Tris borate buffer and were visualized by ethidium bromide staining.

    Antiviral Assay:

    Article Title: Rapid Identification of Rhodococcus equi by a PCR Assay Targeting the choE Gene
    Article Snippet: The sequences were analyzed by using the BLAST ( ) network service from the National Center for Biotechnology Information (Bethesda, Md.) and were aligned by use of the AlignX program (InforMax, Bethesda, Md.). .. For restriction fragment length polymorphism (RFLP) analysis, the choE amplicons were purified by use of the Gel Extraction Purification kit (Qiagen) and were subsequently digested with the following restriction enzymes: Ava I, Bam HI, Bgl I, Hin PI, Pvu I, Xho I, and Xmn I (New England Biolabs, Beverly, Mass.). .. The digestion products were resolved by electrophoresis on 1.2% agarose gels in Tris borate buffer and were visualized by ethidium bromide staining.

    Plasmid Preparation:

    Article Title: Characterization of the Canine MHC Class I DLA-88*50101 Peptide Binding Motif as a Prerequisite for Canine T Cell Immunotherapy
    Article Snippet: The Epstein-Barr virus-positive (EBV) B-lymphoblastoid cell line JY [ ] was used for flow cytometric analyses as well as for peptide binding assays. .. Linearization of DLA-88* Plasmid Encoded with DLA-88*50101 For linearization, 30 μg pcDNA3.1(+) vector with DLA-88*50101 coding sequence insert (GeneArt) was mixed with 5 μl NEB3.1 Buffer (New England, BioLabs), 0.5 μl 100X BSA (New England, BioLabs) and 2 μl PvuI (New England, BioLabs). .. HPLC-H2 O was added to a total volume of 50 μl, followed by incubation at 37°C for 16 h. Ethanol precipitation was used to concentrate and purify the plasmid DNA: 50 μl HPLC-H2 O, 50 μl 7.5 M NHyOAC and 300 μl 100% ethanol were added to each sample.

    Article Title: Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing
    Article Snippet: Transcribed crRNAs were subsequently purified with the miRNeasy Mini Kit (Qiagen). .. In vitro cleavage reactions consisted of 2.5 nM PvuI-linearized substrate plasmid, 300 nM crRNA, and 200 nM purified Cas12a protein in cleavage buffer (10 mM Hepes pH 7.5, 150 mM NaCl and 5 mM MgCl2), and were performed at 37 °C unless otherwise indicated. .. Plasmid substrates for temperature tolerance assays encoded the PAMDA site 2 spacer with a TTTA PAM (in and ).

    Sequencing:

    Article Title: Characterization of the Canine MHC Class I DLA-88*50101 Peptide Binding Motif as a Prerequisite for Canine T Cell Immunotherapy
    Article Snippet: The Epstein-Barr virus-positive (EBV) B-lymphoblastoid cell line JY [ ] was used for flow cytometric analyses as well as for peptide binding assays. .. Linearization of DLA-88* Plasmid Encoded with DLA-88*50101 For linearization, 30 μg pcDNA3.1(+) vector with DLA-88*50101 coding sequence insert (GeneArt) was mixed with 5 μl NEB3.1 Buffer (New England, BioLabs), 0.5 μl 100X BSA (New England, BioLabs) and 2 μl PvuI (New England, BioLabs). .. HPLC-H2 O was added to a total volume of 50 μl, followed by incubation at 37°C for 16 h. Ethanol precipitation was used to concentrate and purify the plasmid DNA: 50 μl HPLC-H2 O, 50 μl 7.5 M NHyOAC and 300 μl 100% ethanol were added to each sample.

    In Vitro:

    Article Title: Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing
    Article Snippet: Transcribed crRNAs were subsequently purified with the miRNeasy Mini Kit (Qiagen). .. In vitro cleavage reactions consisted of 2.5 nM PvuI-linearized substrate plasmid, 300 nM crRNA, and 200 nM purified Cas12a protein in cleavage buffer (10 mM Hepes pH 7.5, 150 mM NaCl and 5 mM MgCl2), and were performed at 37 °C unless otherwise indicated. .. Plasmid substrates for temperature tolerance assays encoded the PAMDA site 2 spacer with a TTTA PAM (in and ).

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  • 98
    New England Biolabs pvu i
    RFLP patterns of choE amplicons from three R. equi isolates (strains 15, 65, and 115, of equine, human, and environmental origin, respectively, in that order for the digestions with each of the enzymes) digested with <t>Pvu</t> I (lanes 1 to 3), Xho I (lanes 4 to 6), Xmn I (lanes 7 to 9), Ava I (lanes 10 to 12), Bam HI (lanes 13 to 15), <t>Bgl</t> I (lanes 16 to 18), and Hin PI (lanes 19 to 21). Lane M, DNA size marker.
    Pvu I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pvu i/product/New England Biolabs
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pvu i - by Bioz Stars, 2021-06
    98/100 stars
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    RFLP patterns of choE amplicons from three R. equi isolates (strains 15, 65, and 115, of equine, human, and environmental origin, respectively, in that order for the digestions with each of the enzymes) digested with Pvu I (lanes 1 to 3), Xho I (lanes 4 to 6), Xmn I (lanes 7 to 9), Ava I (lanes 10 to 12), Bam HI (lanes 13 to 15), Bgl I (lanes 16 to 18), and Hin PI (lanes 19 to 21). Lane M, DNA size marker.

    Journal: Journal of Clinical Microbiology

    Article Title: Rapid Identification of Rhodococcus equi by a PCR Assay Targeting the choE Gene

    doi: 10.1128/JCM.41.7.3241-3245.2003

    Figure Lengend Snippet: RFLP patterns of choE amplicons from three R. equi isolates (strains 15, 65, and 115, of equine, human, and environmental origin, respectively, in that order for the digestions with each of the enzymes) digested with Pvu I (lanes 1 to 3), Xho I (lanes 4 to 6), Xmn I (lanes 7 to 9), Ava I (lanes 10 to 12), Bam HI (lanes 13 to 15), Bgl I (lanes 16 to 18), and Hin PI (lanes 19 to 21). Lane M, DNA size marker.

    Article Snippet: For restriction fragment length polymorphism (RFLP) analysis, the choE amplicons were purified by use of the Gel Extraction Purification kit (Qiagen) and were subsequently digested with the following restriction enzymes: Ava I, Bam HI, Bgl I, Hin PI, Pvu I, Xho I, and Xmn I (New England Biolabs, Beverly, Mass.).

    Techniques: Antiviral Assay, Marker

    Schematic of DNA oligomer preparation. (a) Purified pET-21b plasmids were enzymatically digested by selected pairs of ScaI, PvuI, Pst I, BsaI, and EcoNI restriction enzymes, producing fragments of different lengths. The target DNA sequence complementary to the PNA probe is located beginning at plasmid position 4427 (orange band). Plasmid digestion by ScaI and PvuI produced a 110-base, target-containing fragment, T1. Plasmid digestion by PvuI and PstI produced a 125-base, target-free control fragment, C1. Other fragments were produced similarly: T2 (235 bases) using ScaI and PstI, T3 (419 bases) using ScaI and BsaI, T4 (1613 bases) using by PvuI and EcoNI), C2 (184 bases) using PstI and BsaI, C3 (309 bases) using PvuI and BsaI, and C4 (1503 bases) using ScaI and EcoNI. (b) Following digestion, the DNA was isolated by gel electrophoresis, extracted, and purified. (c) Purified double-stranded DNA was denatured and hybridized with bead–PNA probe conjugates. (d) DNA–PNA–bead mixture was injected into the micropipette for electrical detection.

    Journal: Analytical Chemistry

    Article Title: Sequence-Specific DNA Detection at 10 fM by Electromechanical Signal Transduction

    doi: 10.1021/ac5021408

    Figure Lengend Snippet: Schematic of DNA oligomer preparation. (a) Purified pET-21b plasmids were enzymatically digested by selected pairs of ScaI, PvuI, Pst I, BsaI, and EcoNI restriction enzymes, producing fragments of different lengths. The target DNA sequence complementary to the PNA probe is located beginning at plasmid position 4427 (orange band). Plasmid digestion by ScaI and PvuI produced a 110-base, target-containing fragment, T1. Plasmid digestion by PvuI and PstI produced a 125-base, target-free control fragment, C1. Other fragments were produced similarly: T2 (235 bases) using ScaI and PstI, T3 (419 bases) using ScaI and BsaI, T4 (1613 bases) using by PvuI and EcoNI), C2 (184 bases) using PstI and BsaI, C3 (309 bases) using PvuI and BsaI, and C4 (1503 bases) using ScaI and EcoNI. (b) Following digestion, the DNA was isolated by gel electrophoresis, extracted, and purified. (c) Purified double-stranded DNA was denatured and hybridized with bead–PNA probe conjugates. (d) DNA–PNA–bead mixture was injected into the micropipette for electrical detection.

    Article Snippet: Restriction endonucleases ScaI, PvuI, PstI, BsaI, and EcoNI were obtained from New England BioLabs, Inc. (Ipswich, MA).

    Techniques: Purification, Positron Emission Tomography, Sequencing, Plasmid Preparation, Produced, Isolation, Nucleic Acid Electrophoresis, Injection