pvui restriction endonucleases  (New England Biolabs)


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    Structured Review

    New England Biolabs pvui restriction endonucleases
    Pvui Restriction Endonucleases, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 78/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pvui restriction endonucleases/product/New England Biolabs
    Average 78 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pvui restriction endonucleases - by Bioz Stars, 2019-12
    78/100 stars

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    Polymerase Chain Reaction:

    Article Title: Biocompatible artificial DNA linker that is read through by DNA polymerases and is functional in Escherichia coli
    Article Snippet: PCR cycling conditions were: 94 °C (initial denaturation) for 1.5 min then 35 cycles of 94 °C (denaturation) for 30 s, 46.5 °C (annealing) for 30 s and 72 °C (extension) for 30 s. The reaction was held at 72 °C for 5 min after the 35 cycles. .. The PCR products were digested with ScaI HF and PvuI restriction endonucleases (NEB, Cat. No. R3122 and R0150) according to the manufacturer’s protocol and was purified using QIAquick PCR purification kit (QIAGEN, Cat. No. 28106). .. The Luciferase T7 control plasmid (Promega, Cat. No. L4821) was also digested with ScaI HF and PvuI, and treated with thermosensitive alkaline phosphatase (Promega, Cat. No. M9910) to remove the 5′-phosphate groups from the linearized plasmid DNA, thus preventing recircularization during ligation.

    Gel Extraction:

    Article Title: Biocompatible artificial DNA linker that is read through by DNA polymerases and is functional in Escherichia coli
    Article Snippet: The PCR products were digested with ScaI HF and PvuI restriction endonucleases (NEB, Cat. No. R3122 and R0150) according to the manufacturer’s protocol and was purified using QIAquick PCR purification kit (QIAGEN, Cat. No. 28106). .. The Luciferase T7 control plasmid (Promega, Cat. No. L4821) was also digested with ScaI HF and PvuI, and treated with thermosensitive alkaline phosphatase (Promega, Cat. No. M9910) to remove the 5′-phosphate groups from the linearized plasmid DNA, thus preventing recircularization during ligation.

    Plasmid Preparation:

    Article Title: Biocompatible artificial DNA linker that is read through by DNA polymerases and is functional in Escherichia coli
    Article Snippet: Paragraph title: Restriction digestion of PCR product and vector. ... The PCR products were digested with ScaI HF and PvuI restriction endonucleases (NEB, Cat. No. R3122 and R0150) according to the manufacturer’s protocol and was purified using QIAquick PCR purification kit (QIAGEN, Cat. No. 28106).

    Purification:

    Article Title: Biocompatible artificial DNA linker that is read through by DNA polymerases and is functional in Escherichia coli
    Article Snippet: PCR cycling conditions were: 94 °C (initial denaturation) for 1.5 min then 35 cycles of 94 °C (denaturation) for 30 s, 46.5 °C (annealing) for 30 s and 72 °C (extension) for 30 s. The reaction was held at 72 °C for 5 min after the 35 cycles. .. The PCR products were digested with ScaI HF and PvuI restriction endonucleases (NEB, Cat. No. R3122 and R0150) according to the manufacturer’s protocol and was purified using QIAquick PCR purification kit (QIAGEN, Cat. No. 28106). .. The Luciferase T7 control plasmid (Promega, Cat. No. L4821) was also digested with ScaI HF and PvuI, and treated with thermosensitive alkaline phosphatase (Promega, Cat. No. M9910) to remove the 5′-phosphate groups from the linearized plasmid DNA, thus preventing recircularization during ligation.

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  • 78
    New England Biolabs pvui restriction endonucleases
    Pvui Restriction Endonucleases, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 78/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pvui restriction endonucleases/product/New England Biolabs
    Average 78 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pvui restriction endonucleases - by Bioz Stars, 2019-12
    78/100 stars
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