Journal: Journal of Clinical Microbiology
Article Title: Rapid Identification of Rhodococcus equi by a PCR Assay Targeting the choE Gene
doi: 10.1128/JCM.41.7.3241-3245.2003
Figure Lengend Snippet: RFLP patterns of choE amplicons from three R. equi isolates (strains 15, 65, and 115, of equine, human, and environmental origin, respectively, in that order for the digestions with each of the enzymes) digested with Pvu I (lanes 1 to 3), Xho I (lanes 4 to 6), Xmn I (lanes 7 to 9), Ava I (lanes 10 to 12), Bam HI (lanes 13 to 15), Bgl I (lanes 16 to 18), and Hin PI (lanes 19 to 21). Lane M, DNA size marker.
Article Snippet: For restriction fragment length polymorphism (RFLP) analysis, the choE amplicons were purified by use of the Gel Extraction Purification kit (Qiagen) and were subsequently digested with the following restriction enzymes: Ava I, Bam HI, Bgl I, Hin PI, Pvu I, Xho I, and Xmn I (New England Biolabs, Beverly, Mass.).
Techniques: Antiviral Assay, Marker