bgli  (New England Biolabs)


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    Name:
    BglI
    Description:
    BglI 10 000 units
    Catalog Number:
    R0143L
    Price:
    249
    Category:
    Restriction Enzymes
    Size:
    10 000 units
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    Structured Review

    New England Biolabs bgli
    BglI
    BglI 10 000 units
    https://www.bioz.com/result/bgli/product/New England Biolabs
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bgli - by Bioz Stars, 2021-06
    96/100 stars

    Images

    1) Product Images from "Recursive Directional Ligation by Plasmid Reconstruction allows Rapid and Seamless Cloning of Oligomeric Genes"

    Article Title: Recursive Directional Ligation by Plasmid Reconstruction allows Rapid and Seamless Cloning of Oligomeric Genes

    Journal: Biomacromolecules

    doi: 10.1021/bm901387t

    (A) Recursive directional ligation by plasmid reconstruction (PRe-RDL). One round in PRe-RDL involves: (1) purifying the ELP-containing DNA fragment from the parent vector that is digested with AcuI and BglI; and (2) purifying the ELP-containing fragment
    Figure Legend Snippet: (A) Recursive directional ligation by plasmid reconstruction (PRe-RDL). One round in PRe-RDL involves: (1) purifying the ELP-containing DNA fragment from the parent vector that is digested with AcuI and BglI; and (2) purifying the ELP-containing fragment

    Techniques Used: Ligation, Plasmid Preparation

    2) Product Images from "Topography of Bovine Papillomavirus E2 Protein on the Viral Genome During the Cell Cycle"

    Article Title: Topography of Bovine Papillomavirus E2 Protein on the Viral Genome During the Cell Cycle

    Journal: Virology

    doi: 10.1016/j.virol.2009.07.037

    A3 cells were synchronized and E2 was immunoprecipitated without digestion (lane1) or digested with BglI and PflM1 (lane 2). Lane 3 is undigested ChIP sample using rabbit pre-immune serum, and Input DNA diluted 1:10 is also shown (lane 4). PCR was performed
    Figure Legend Snippet: A3 cells were synchronized and E2 was immunoprecipitated without digestion (lane1) or digested with BglI and PflM1 (lane 2). Lane 3 is undigested ChIP sample using rabbit pre-immune serum, and Input DNA diluted 1:10 is also shown (lane 4). PCR was performed

    Techniques Used: Immunoprecipitation, Chromatin Immunoprecipitation, Polymerase Chain Reaction

    3) Product Images from "Expression and Differentiation between OCT4A and Its Pseudogenes in Human ESCs and Differentiated Adult Somatic Cells"

    Article Title: Expression and Differentiation between OCT4A and Its Pseudogenes in Human ESCs and Differentiated Adult Somatic Cells

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0089546

    Schematic representation of restriction sites in 646-PCR amplicon. Specific restriction sites were used to distinguish between embryonic OCT4A transcript and different pseudogenes. Red arrows show restriction sites. ApaI restriction site is present only in embryonic OCT4A and can be used to distinguish embryonic form from all six pseudogenes; after restriction, a 146 bp and 500 bp long fragments are produced. HinfI digestion results in several smaller fragments among which the 434 bp fragment is specific only for OCT4-pg1. BglI digests only OCT4-pg3 into two fragments of 412 bp and 232 bp. XhoI does not digest OCT4-pg4.
    Figure Legend Snippet: Schematic representation of restriction sites in 646-PCR amplicon. Specific restriction sites were used to distinguish between embryonic OCT4A transcript and different pseudogenes. Red arrows show restriction sites. ApaI restriction site is present only in embryonic OCT4A and can be used to distinguish embryonic form from all six pseudogenes; after restriction, a 146 bp and 500 bp long fragments are produced. HinfI digestion results in several smaller fragments among which the 434 bp fragment is specific only for OCT4-pg1. BglI digests only OCT4-pg3 into two fragments of 412 bp and 232 bp. XhoI does not digest OCT4-pg4.

    Techniques Used: Polymerase Chain Reaction, Amplification, Produced

    4) Product Images from "Topography of Bovine Papillomavirus E2 Protein on the Viral Genome During the Cell Cycle"

    Article Title: Topography of Bovine Papillomavirus E2 Protein on the Viral Genome During the Cell Cycle

    Journal: Virology

    doi: 10.1016/j.virol.2009.07.037

    A3 cells were synchronized and E2 was immunoprecipitated without digestion (lane1) or digested with BglI and PflM1 (lane 2). Lane 3 is undigested ChIP sample using rabbit pre-immune serum, and Input DNA diluted 1:10 is also shown (lane 4). PCR was performed
    Figure Legend Snippet: A3 cells were synchronized and E2 was immunoprecipitated without digestion (lane1) or digested with BglI and PflM1 (lane 2). Lane 3 is undigested ChIP sample using rabbit pre-immune serum, and Input DNA diluted 1:10 is also shown (lane 4). PCR was performed

    Techniques Used: Immunoprecipitation, Chromatin Immunoprecipitation, Polymerase Chain Reaction

    5) Product Images from "Defining characteristics of Tn5 Transposase non-specific DNA binding"

    Article Title: Defining characteristics of Tn5 Transposase non-specific DNA binding

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gkl179

    Single ES substrates of differing lengths are cleaved with variable rate constants. ( A ) A partial restriction map of the plasmid (pWSR6103) used to create substrates for in vitro transposition reactions is shown. The Tnp ES is represented as a black arrow. This plasmid was digested with PflMI and either PvuII, BglI, NarI, NdeI, AatII or XmnI to create substrates varying in size from 485 to 1183 bp. Each restriction fragment contained 395 bp of transposon (Tn) DNA and varying lengths of donor backbone (dbb) DNA as shown. The location of the transposon ES in each substrate is marked with a black arrow. ( B ) A schematic of the in vitro transposition reactions with single-ended substrates is shown. Each substrate DNA was incubated (together with non-specific DNA remaining from the restriction digest) with Tnp and MgAc at 37°C. Time points were taken from 0 to 8 h. Following PEC formation, the substrate was cleaved into two products, the dbb and Tn DNA. In this figure, the single ended substrate DNA is shown as two parallel lines containing a transposon ES (gray box). The cleavage site is marked with +1. The non-specific DNA remaining from the restriction digest is shown as linear double stranded DNA. Both product DNAs are appropriately labeled and other reaction components are described as in Figure 4 . ( C ) Each time point was run on an appropriate agarose gel to separate the full-length, unreacted substrate from the dbb and Tn DNA products. In this representative gel of the 555 bp substrate, time points are shown in lanes 3–13 and DNA size markers are shown in lanes 1 and 2. The substrate, dbb and Tn DNAs are represented as in (B). ( D ) The percentage of substrates cleaved was determined for each time point as described in the Materials and Methods. The mean percentage cleaved at each time point was calculated from at least three independent experiments and was then plotted (together with error bars representing the standard error) versus time and the data were fit to a one-phase exponential equation. The plot shown here represents data for the 555 bp substrate. In vitro transposition reactions and analysis were performed in this fashion for each of the six single end substrates. ( E ) k obs,cleavage and the standard error (SE) of this value were calculated from the fits described in (D). These are shown for each of the six substrates tested. ( F ) To better visualize the effect of substrate length on k obs,cleavage , k obs,cleavage was plotted versus substrate length for each substrate. The error bars represent the standard error of k obs,cleavage for each substrate.
    Figure Legend Snippet: Single ES substrates of differing lengths are cleaved with variable rate constants. ( A ) A partial restriction map of the plasmid (pWSR6103) used to create substrates for in vitro transposition reactions is shown. The Tnp ES is represented as a black arrow. This plasmid was digested with PflMI and either PvuII, BglI, NarI, NdeI, AatII or XmnI to create substrates varying in size from 485 to 1183 bp. Each restriction fragment contained 395 bp of transposon (Tn) DNA and varying lengths of donor backbone (dbb) DNA as shown. The location of the transposon ES in each substrate is marked with a black arrow. ( B ) A schematic of the in vitro transposition reactions with single-ended substrates is shown. Each substrate DNA was incubated (together with non-specific DNA remaining from the restriction digest) with Tnp and MgAc at 37°C. Time points were taken from 0 to 8 h. Following PEC formation, the substrate was cleaved into two products, the dbb and Tn DNA. In this figure, the single ended substrate DNA is shown as two parallel lines containing a transposon ES (gray box). The cleavage site is marked with +1. The non-specific DNA remaining from the restriction digest is shown as linear double stranded DNA. Both product DNAs are appropriately labeled and other reaction components are described as in Figure 4 . ( C ) Each time point was run on an appropriate agarose gel to separate the full-length, unreacted substrate from the dbb and Tn DNA products. In this representative gel of the 555 bp substrate, time points are shown in lanes 3–13 and DNA size markers are shown in lanes 1 and 2. The substrate, dbb and Tn DNAs are represented as in (B). ( D ) The percentage of substrates cleaved was determined for each time point as described in the Materials and Methods. The mean percentage cleaved at each time point was calculated from at least three independent experiments and was then plotted (together with error bars representing the standard error) versus time and the data were fit to a one-phase exponential equation. The plot shown here represents data for the 555 bp substrate. In vitro transposition reactions and analysis were performed in this fashion for each of the six single end substrates. ( E ) k obs,cleavage and the standard error (SE) of this value were calculated from the fits described in (D). These are shown for each of the six substrates tested. ( F ) To better visualize the effect of substrate length on k obs,cleavage , k obs,cleavage was plotted versus substrate length for each substrate. The error bars represent the standard error of k obs,cleavage for each substrate.

    Techniques Used: Plasmid Preparation, In Vitro, Incubation, Labeling, Agarose Gel Electrophoresis

    Related Articles

    Immunoprecipitation:

    Article Title: Topography of Bovine Papillomavirus E2 Protein on the Viral Genome During the Cell Cycle
    Article Snippet: For restriction enzyme digestion coupled ChIP (RED-ChIP), cells were treated with 0.33% formaldehyde and ChIP was performed according to the manufacturer's instructions. .. Following immunoprecipitation and washing, samples were resuspended in 100 μl of the appropriate restriction enzyme digest buffer, 1% bovine serum albumin (BSA) and either 30 units of BanII (New England Biolabs), 70 units high concentrate BglI (Promega), 70 units of BglI and 8 units PflM1 (New England Biolabs), or no enzyme. .. Following overnight digestion, samples were washed twice with TE buffer and processed using the standard ChIP protocol.

    Article Title: Topography of Bovine Papillomavirus E2 Protein on the Viral Genome During the Cell Cycle
    Article Snippet: For restriction enzyme digestion coupled ChIP (RED-ChIP), cells were treated with 0.33% formaldehyde and ChIP was performed according to the manufacturer's instructions. .. Following immunoprecipitation and washing, samples were resuspended in 100 μl of the appropriate restriction enzyme digest buffer, 1% bovine serum albumin (BSA) and either 30 units of BanII (New England Biolabs), 70 units high concentrate BglI (Promega), 70 units of BglI and 8 units PflM1 (New England Biolabs), or no enzyme. .. Following overnight digestion, samples were washed twice with TE buffer and processed using the standard ChIP protocol.

    Polymerase Chain Reaction:

    Article Title: Strategy for Systematic Assembly of Large RNA and DNA Genomes: Transmissible Gastroenteritis Virus Model
    Article Snippet: Following 28 cycles of amplification with Taq polymerase (Expand Long Kit; Roche Biochemical), the PCR products were separated and isolated from agarose gels. .. PCR amplicons were either subcloned directly into pGemT cloning vectors for sequencing or digested with Bgl I or Bst XI restriction endonuclease according to the manufacturer's directions (NEB). ..

    Article Title: Rapidly discriminate commercial medicinal Pulsatilla chinensis (Bge.) Regel from its adulterants using ITS2 barcoding and specific PCR-RFLP assay
    Article Snippet: The PCR conditions were 1 cycle of 95 °C for 3 min, 35 cycles of 94 °C for 30 se, 62 °C for 20 s, and 72 °C for 20 s followed by 1 cycle of 72 °C for 10 min. PCR products (6 μL of each) were visualized by 2% agarose gel electrophoresis in 1 × TAE buffer for 20 minutes at 120 V. Restriction enzymes were predicted by Vector NTI 10.3.0 (Invitrogen, Carlsbad, CA, USA) and Bgl I was selected as the best. .. Digestion was performed by incubating 15 μL of PCR product with 10 U of Bgl I (NEB) and 2.5 μL 10 × buffer in a final volume of 25 μL at 37 °C for 1 h, and then restriction fragments were separated by 3% agarose gel electrophoresis in 1 × TAE buffer for 30 min at 120 V. ..

    Clone Assay:

    Article Title: Strategy for Systematic Assembly of Large RNA and DNA Genomes: Transmissible Gastroenteritis Virus Model
    Article Snippet: Following 28 cycles of amplification with Taq polymerase (Expand Long Kit; Roche Biochemical), the PCR products were separated and isolated from agarose gels. .. PCR amplicons were either subcloned directly into pGemT cloning vectors for sequencing or digested with Bgl I or Bst XI restriction endonuclease according to the manufacturer's directions (NEB). ..

    Sequencing:

    Article Title: Strategy for Systematic Assembly of Large RNA and DNA Genomes: Transmissible Gastroenteritis Virus Model
    Article Snippet: Following 28 cycles of amplification with Taq polymerase (Expand Long Kit; Roche Biochemical), the PCR products were separated and isolated from agarose gels. .. PCR amplicons were either subcloned directly into pGemT cloning vectors for sequencing or digested with Bgl I or Bst XI restriction endonuclease according to the manufacturer's directions (NEB). ..

    Agarose Gel Electrophoresis:

    Article Title: Rapidly discriminate commercial medicinal Pulsatilla chinensis (Bge.) Regel from its adulterants using ITS2 barcoding and specific PCR-RFLP assay
    Article Snippet: The PCR conditions were 1 cycle of 95 °C for 3 min, 35 cycles of 94 °C for 30 se, 62 °C for 20 s, and 72 °C for 20 s followed by 1 cycle of 72 °C for 10 min. PCR products (6 μL of each) were visualized by 2% agarose gel electrophoresis in 1 × TAE buffer for 20 minutes at 120 V. Restriction enzymes were predicted by Vector NTI 10.3.0 (Invitrogen, Carlsbad, CA, USA) and Bgl I was selected as the best. .. Digestion was performed by incubating 15 μL of PCR product with 10 U of Bgl I (NEB) and 2.5 μL 10 × buffer in a final volume of 25 μL at 37 °C for 1 h, and then restriction fragments were separated by 3% agarose gel electrophoresis in 1 × TAE buffer for 30 min at 120 V. ..

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    New England Biolabs bgli
    (A) Recursive directional ligation by plasmid reconstruction (PRe-RDL). One round in PRe-RDL involves: (1) purifying the ELP-containing DNA fragment from the parent vector that is digested with <t>AcuI</t> and <t>BglI;</t> and (2) purifying the ELP-containing fragment
    Bgli, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bgli/product/New England Biolabs
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    (A) Recursive directional ligation by plasmid reconstruction (PRe-RDL). One round in PRe-RDL involves: (1) purifying the ELP-containing DNA fragment from the parent vector that is digested with AcuI and BglI; and (2) purifying the ELP-containing fragment

    Journal: Biomacromolecules

    Article Title: Recursive Directional Ligation by Plasmid Reconstruction allows Rapid and Seamless Cloning of Oligomeric Genes

    doi: 10.1021/bm901387t

    Figure Lengend Snippet: (A) Recursive directional ligation by plasmid reconstruction (PRe-RDL). One round in PRe-RDL involves: (1) purifying the ELP-containing DNA fragment from the parent vector that is digested with AcuI and BglI; and (2) purifying the ELP-containing fragment

    Article Snippet: Using the dimerization of the 30 repeat fragment of ELP1 in the second round of PRe-RDL as an example, the designated ‘A’ fragment was obtained by digestion of 4 μg of ELP1 -30 with 10 U AcuI and 40 U BglI for 3 hours at 37°C (see ) in NEB Buffer 2 (New England Biolabs; Ipswich, MA).

    Techniques: Ligation, Plasmid Preparation

    Marker mutations are present in virus derived from the infectious construct. Cultures of ST cells were infected with wild-type (WT) TGEV or plaque-purified icTGEV isolates derived from the infectious construct. Intracellular RNA was isolated, and RT-PCR was performed using primer pairs that asymmetrically flank each of the unique Bgl I- Bst XI junctions inserted into the infectious construct. (A) Wild-type TGEV. (B) icTGEV-1 (passage 1). (C) icTGEV-3 (passage 3). In panel C, a larger ∼1.6-kb wild-type TGEV amplicon spanning the B1-B2 junction was also treated with Bst ]). Arrows indicate cleaved DNA intermediates.

    Journal: Journal of Virology

    Article Title: Strategy for Systematic Assembly of Large RNA and DNA Genomes: Transmissible Gastroenteritis Virus Model

    doi:

    Figure Lengend Snippet: Marker mutations are present in virus derived from the infectious construct. Cultures of ST cells were infected with wild-type (WT) TGEV or plaque-purified icTGEV isolates derived from the infectious construct. Intracellular RNA was isolated, and RT-PCR was performed using primer pairs that asymmetrically flank each of the unique Bgl I- Bst XI junctions inserted into the infectious construct. (A) Wild-type TGEV. (B) icTGEV-1 (passage 1). (C) icTGEV-3 (passage 3). In panel C, a larger ∼1.6-kb wild-type TGEV amplicon spanning the B1-B2 junction was also treated with Bst ]). Arrows indicate cleaved DNA intermediates.

    Article Snippet: PCR amplicons were either subcloned directly into pGemT cloning vectors for sequencing or digested with Bgl I or Bst XI restriction endonuclease according to the manufacturer's directions (NEB).

    Techniques: Marker, Derivative Assay, Construct, Infection, Purification, Isolation, Reverse Transcription Polymerase Chain Reaction, Amplification

    Assembly of the TGEV full-length construct. (A) The various TGEV plasmid DNAs were digested with Bgl I, Bst XI, or Not I, and the appropriate-sized products were isolated from agarose gels as described in the text. The TGEV A and B1 fragments, TGEV B2 and C3 fragments, or TGEV DE-1 and F fragments were ligated at 16°C overnight in separate reactions. Appropriate-sized products were isolated from agarose gels. (B) A+B1. (C) B2+C. (D) DE-1+F. Following purification from agarose gels, the purified products are shown in panel A as well.

    Journal: Journal of Virology

    Article Title: Strategy for Systematic Assembly of Large RNA and DNA Genomes: Transmissible Gastroenteritis Virus Model

    doi:

    Figure Lengend Snippet: Assembly of the TGEV full-length construct. (A) The various TGEV plasmid DNAs were digested with Bgl I, Bst XI, or Not I, and the appropriate-sized products were isolated from agarose gels as described in the text. The TGEV A and B1 fragments, TGEV B2 and C3 fragments, or TGEV DE-1 and F fragments were ligated at 16°C overnight in separate reactions. Appropriate-sized products were isolated from agarose gels. (B) A+B1. (C) B2+C. (D) DE-1+F. Following purification from agarose gels, the purified products are shown in panel A as well.

    Article Snippet: PCR amplicons were either subcloned directly into pGemT cloning vectors for sequencing or digested with Bgl I or Bst XI restriction endonuclease according to the manufacturer's directions (NEB).

    Techniques: Construct, Plasmid Preparation, Isolation, Purification

    Fig. 2. Mapping of σ-like mp1 replication intermediates with one tail. Plasmid molecules from fractions 2, 3 and 4 were combined and linearized with Bgl I, Pvu I and Sma I, respectively. EM investigation of 100 molecules each revealed symmetric simple Ys ( A ) and asymmetric simple Ys ( B ). The location of the origins dso1 and dso2 was deduced as described in the text. The x -axis corresponds to positions on the plasmid map as indicated in the model above. Examples are shown in the right panel. ( C ) Example and model of an uncut mp1 RC. Bar = 0.5 kb.

    Journal: The EMBO Journal

    Article Title: R-loop-dependent rolling-circle replication and a new model for DNA concatemer resolution by mitochondrial plasmid mp1

    doi: 10.1093/emboj/cdf311

    Figure Lengend Snippet: Fig. 2. Mapping of σ-like mp1 replication intermediates with one tail. Plasmid molecules from fractions 2, 3 and 4 were combined and linearized with Bgl I, Pvu I and Sma I, respectively. EM investigation of 100 molecules each revealed symmetric simple Ys ( A ) and asymmetric simple Ys ( B ). The location of the origins dso1 and dso2 was deduced as described in the text. The x -axis corresponds to positions on the plasmid map as indicated in the model above. Examples are shown in the right panel. ( C ) Example and model of an uncut mp1 RC. Bar = 0.5 kb.

    Article Snippet: For mapping experiments, mp1 was digested with Pvu II, Sma I or Bgl I (NEB, Beverly, CA).

    Techniques: Plasmid Preparation

    Fig. 7. EM of highly complex in vivo mp1 replication and recombination intermediates. ( A ) Molecules from fraction 8 were linearized with Bgl I, Pvu I and Sma I, respectively. EM of 150 molecules mainly revealed symmetric simple Ys and double Ys. Examples are shown in the right panel. (B–F) Examples and explanation of uncut complex mp1 concatemers. ( B ) Open circle with a branched double-stranded tail of 1.3 kb. ( C ) Open circle with a 1.1 kb tail and a D-loop. ( D ) Highly branched mp1 molecule (total size 8.9 kb). ( E ) Model for the origin of this structure. The upper molecule may represent a double RC initiated at dso1 and dso2 . Both RC tails are invaded by two other RCs (bottom). ( F ) Highly branched mp1 molecule (total size is 26.3 kb) that represents a double RC (reinitiated at dso1 ), recombining with a linear 10mer at dso1 . Bars = 0.5 kb.

    Journal: The EMBO Journal

    Article Title: R-loop-dependent rolling-circle replication and a new model for DNA concatemer resolution by mitochondrial plasmid mp1

    doi: 10.1093/emboj/cdf311

    Figure Lengend Snippet: Fig. 7. EM of highly complex in vivo mp1 replication and recombination intermediates. ( A ) Molecules from fraction 8 were linearized with Bgl I, Pvu I and Sma I, respectively. EM of 150 molecules mainly revealed symmetric simple Ys and double Ys. Examples are shown in the right panel. (B–F) Examples and explanation of uncut complex mp1 concatemers. ( B ) Open circle with a branched double-stranded tail of 1.3 kb. ( C ) Open circle with a 1.1 kb tail and a D-loop. ( D ) Highly branched mp1 molecule (total size 8.9 kb). ( E ) Model for the origin of this structure. The upper molecule may represent a double RC initiated at dso1 and dso2 . Both RC tails are invaded by two other RCs (bottom). ( F ) Highly branched mp1 molecule (total size is 26.3 kb) that represents a double RC (reinitiated at dso1 ), recombining with a linear 10mer at dso1 . Bars = 0.5 kb.

    Article Snippet: For mapping experiments, mp1 was digested with Pvu II, Sma I or Bgl I (NEB, Beverly, CA).

    Techniques: In Vivo

    Fig. 3. Mapping of σ-like mp1 intermediates with two tails (double RCs). ( A ) Plasmid DNA from fractions 5 and 6 was combined and linearized with Bgl I, Pvu I and Sma I, respectively. EM of 150 molecules in each digest revealed various double Ys. The location of the origins dso1 and dso2 was mapped. Examples are shown in the right panel. ( B ) Uncut double RCs initiated at dso1 and dso2 are explained in the model. Bar = 0.5 kb.

    Journal: The EMBO Journal

    Article Title: R-loop-dependent rolling-circle replication and a new model for DNA concatemer resolution by mitochondrial plasmid mp1

    doi: 10.1093/emboj/cdf311

    Figure Lengend Snippet: Fig. 3. Mapping of σ-like mp1 intermediates with two tails (double RCs). ( A ) Plasmid DNA from fractions 5 and 6 was combined and linearized with Bgl I, Pvu I and Sma I, respectively. EM of 150 molecules in each digest revealed various double Ys. The location of the origins dso1 and dso2 was mapped. Examples are shown in the right panel. ( B ) Uncut double RCs initiated at dso1 and dso2 are explained in the model. Bar = 0.5 kb.

    Article Snippet: For mapping experiments, mp1 was digested with Pvu II, Sma I or Bgl I (NEB, Beverly, CA).

    Techniques: Plasmid Preparation

    Fig. 6. EM mapping of in vivo mp1 recombination intermediates. ( A ) DNA from fraction 8 was cut with Bgl I, Pvu I and Sma I. EM of 100 molecules in each digest revealed X-shaped mp1 recombinants that mapped to dso1 . Examples of cut ( B ) and uncut mp1 dimers ( C ) containing recombination junctions (arrowheads). Bar = 0.5 kb.

    Journal: The EMBO Journal

    Article Title: R-loop-dependent rolling-circle replication and a new model for DNA concatemer resolution by mitochondrial plasmid mp1

    doi: 10.1093/emboj/cdf311

    Figure Lengend Snippet: Fig. 6. EM mapping of in vivo mp1 recombination intermediates. ( A ) DNA from fraction 8 was cut with Bgl I, Pvu I and Sma I. EM of 100 molecules in each digest revealed X-shaped mp1 recombinants that mapped to dso1 . Examples of cut ( B ) and uncut mp1 dimers ( C ) containing recombination junctions (arrowheads). Bar = 0.5 kb.

    Article Snippet: For mapping experiments, mp1 was digested with Pvu II, Sma I or Bgl I (NEB, Beverly, CA).

    Techniques: In Vivo

    A3 cells were synchronized and E2 was immunoprecipitated without digestion (lane1) or digested with BglI and PflM1 (lane 2). Lane 3 is undigested ChIP sample using rabbit pre-immune serum, and Input DNA diluted 1:10 is also shown (lane 4). PCR was performed

    Journal: Virology

    Article Title: Topography of Bovine Papillomavirus E2 Protein on the Viral Genome During the Cell Cycle

    doi: 10.1016/j.virol.2009.07.037

    Figure Lengend Snippet: A3 cells were synchronized and E2 was immunoprecipitated without digestion (lane1) or digested with BglI and PflM1 (lane 2). Lane 3 is undigested ChIP sample using rabbit pre-immune serum, and Input DNA diluted 1:10 is also shown (lane 4). PCR was performed

    Article Snippet: Following immunoprecipitation and washing, samples were resuspended in 100 μl of the appropriate restriction enzyme digest buffer, 1% bovine serum albumin (BSA) and either 30 units of BanII (New England Biolabs), 70 units high concentrate BglI (Promega), 70 units of BglI and 8 units PflM1 (New England Biolabs), or no enzyme.

    Techniques: Immunoprecipitation, Chromatin Immunoprecipitation, Polymerase Chain Reaction