r0141  (New England Biolabs)


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    Name:
    SmaI
    Description:
    SmaI 10 000 units
    Catalog Number:
    r0141l
    Price:
    261
    Size:
    10 000 units
    Category:
    Restriction Enzymes
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    Structured Review

    New England Biolabs r0141
    SmaI
    SmaI 10 000 units
    https://www.bioz.com/result/r0141/product/New England Biolabs
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    r0141 - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Gammaretroviral vector encoding a fluorescent marker to facilitate detection of reprogrammed human fibroblasts during iPSC generation
    Article Snippet: .. Methylation-sensitive restriction enzyme qPCR (MSRE-qPCR) Genomic DNA (60 ng), isolated using DNeasy Kits, was digested with 5 units of SmaI (methylation sensitive) or MscI (methylation insensitive) or incubated in the absence of restriction enzyme (uncut) in the manufacturer supplied buffer (New England Biolabs, Massachusetts, USA) in a 50 µl reaction volume at 37°C for 4 h. The reaction was terminated by inactivating the enzymes at 80°C for 20 min. An aliquot of the digest (5 µl) containing 6 ng of genomic DNA was then used in qPCR using primers (SK160 and SK161, ) targeting the 5′ Moloney murine leukemia virus LTR and 5′ untranslated region (UTR) ( ). .. The PCR was carried out using SsoAdvanced SYBR Green Supermix (Bio-Rad, Hercules, CA, USA; Catalog no. 1725261) in a Bio-Rad CFX96 thermocycler.

    Agarose Gel Electrophoresis:

    Article Title: Aspartoacylase-LacZ Knockin Mice: An Engineered Model of Canavan Disease
    Article Snippet: .. DNA was digested with ApaI and SmaI (NEB, Ipswich, MA), separated on a 0.7% agarose gel, blotted onto Hybond N+ membrane (GE Healthcare, Dassel, Germany) and probed with a 600 bp 32 P-labelled fragment of the Neo-cassette. .. Mice were genotyped by PCR using Primer 1 as forward primer and Primer 3 as reversed primer resulting in a 336 bp band for the wild-type allele and a 387 bp band for the synthetic allele.

    Article Title: Staphylococcus spp., Streptococcus canis, and Arcanobacterium phocae of healthy Canadian farmed mink and mink with pododermatitis
    Article Snippet: .. Genomic DNA from both species was digested with SmaI (New England BioLabs, Ipswich, Massachusetts, USA) before electrophoresis in a 1.0% agarose gel, using a CHEF-III electrophoresis unit (Bio-Rad Laboratories, Hercules, California, USA) in 0.5× Trisborate-ethylenediamine tetra-acetic acid (EDTA) buffer containing 200 μM thiourea (Fisher Scientific, Fair Lawn, New Jersey, USA). .. For S. delphini, gels were run at 14°C at 6V/cm with an angle of 120° and a linear ramping from 4 s to 10 s for 12 h, followed by 13 s to 20 s for 6 h. For S. canis, gels were run under the same conditions, but with linear ramping from 1 s to 20 s over 16 h. Gel images were analyzed using BioNumerics software v5.1 (Applied Maths, Austin, Texas, USA).

    Plasmid Preparation:

    Article Title: Oncosuppressive Suicide Gene Virotherapy "PVH1-yCD/5-FC" for Pancreatic Peritoneal Carcinomatosis Treatment: NF?B and Akt/PI3K Involvement
    Article Snippet: .. Recombinant PV-H1 virus constructions for yCD and GFP expression The PV-H1-based vector DNA, phH1Δ800 , was linearized with SmaI restriction enzyme and subsequently dephosphorylated by the calf intestinal phosphatase (New England Biolabs-OZYME, Saint Quentin Yveline; France). .. The yCD gene was isolated from yeast genomic DNA (strain D4916 from Sigma-Aldrich) using specific probes: Forward: 5′-att ctcgag c gccaccatgg tgacagggggaatg-3′ containing a Kozak sequence (bold type) and XhoI restriction site (underlined), and Reverse: 5′-att ggatcc ctactcaccaatatcttcaaacc-3′ containing BamH1 restriction site (underlined), and was phosphorylated with T4 Polynucleotide Kinase (New England Biolabs-Ozyme, Montigny-Le-Bretonneux; France).

    Methylation:

    Article Title: Gammaretroviral vector encoding a fluorescent marker to facilitate detection of reprogrammed human fibroblasts during iPSC generation
    Article Snippet: .. Methylation-sensitive restriction enzyme qPCR (MSRE-qPCR) Genomic DNA (60 ng), isolated using DNeasy Kits, was digested with 5 units of SmaI (methylation sensitive) or MscI (methylation insensitive) or incubated in the absence of restriction enzyme (uncut) in the manufacturer supplied buffer (New England Biolabs, Massachusetts, USA) in a 50 µl reaction volume at 37°C for 4 h. The reaction was terminated by inactivating the enzymes at 80°C for 20 min. An aliquot of the digest (5 µl) containing 6 ng of genomic DNA was then used in qPCR using primers (SK160 and SK161, ) targeting the 5′ Moloney murine leukemia virus LTR and 5′ untranslated region (UTR) ( ). .. The PCR was carried out using SsoAdvanced SYBR Green Supermix (Bio-Rad, Hercules, CA, USA; Catalog no. 1725261) in a Bio-Rad CFX96 thermocycler.

    Isolation:

    Article Title: Gammaretroviral vector encoding a fluorescent marker to facilitate detection of reprogrammed human fibroblasts during iPSC generation
    Article Snippet: .. Methylation-sensitive restriction enzyme qPCR (MSRE-qPCR) Genomic DNA (60 ng), isolated using DNeasy Kits, was digested with 5 units of SmaI (methylation sensitive) or MscI (methylation insensitive) or incubated in the absence of restriction enzyme (uncut) in the manufacturer supplied buffer (New England Biolabs, Massachusetts, USA) in a 50 µl reaction volume at 37°C for 4 h. The reaction was terminated by inactivating the enzymes at 80°C for 20 min. An aliquot of the digest (5 µl) containing 6 ng of genomic DNA was then used in qPCR using primers (SK160 and SK161, ) targeting the 5′ Moloney murine leukemia virus LTR and 5′ untranslated region (UTR) ( ). .. The PCR was carried out using SsoAdvanced SYBR Green Supermix (Bio-Rad, Hercules, CA, USA; Catalog no. 1725261) in a Bio-Rad CFX96 thermocycler.

    Article Title: RIP140 in thyroid hormone-repression and chromatin remodeling of Crabp1 gene during adipocyte differentiation
    Article Snippet: .. Isolated nuclei from differentiating 3T3-L1 cells were digested with 100 U of PstI, XhoI, SmaI, SpeI and ApaLI (New England Biolabs) for 30 min. .. The purified genomic DNA was re-digested with 100 U of ApaI completely (for first digestion with XhoI, PstI and SmaI) or 100 U of PstI (for first digestion with SpeI and ApaLI).

    Electrophoresis:

    Article Title: Staphylococcus spp., Streptococcus canis, and Arcanobacterium phocae of healthy Canadian farmed mink and mink with pododermatitis
    Article Snippet: .. Genomic DNA from both species was digested with SmaI (New England BioLabs, Ipswich, Massachusetts, USA) before electrophoresis in a 1.0% agarose gel, using a CHEF-III electrophoresis unit (Bio-Rad Laboratories, Hercules, California, USA) in 0.5× Trisborate-ethylenediamine tetra-acetic acid (EDTA) buffer containing 200 μM thiourea (Fisher Scientific, Fair Lawn, New Jersey, USA). .. For S. delphini, gels were run at 14°C at 6V/cm with an angle of 120° and a linear ramping from 4 s to 10 s for 12 h, followed by 13 s to 20 s for 6 h. For S. canis, gels were run under the same conditions, but with linear ramping from 1 s to 20 s over 16 h. Gel images were analyzed using BioNumerics software v5.1 (Applied Maths, Austin, Texas, USA).

    Incubation:

    Article Title: Gammaretroviral vector encoding a fluorescent marker to facilitate detection of reprogrammed human fibroblasts during iPSC generation
    Article Snippet: .. Methylation-sensitive restriction enzyme qPCR (MSRE-qPCR) Genomic DNA (60 ng), isolated using DNeasy Kits, was digested with 5 units of SmaI (methylation sensitive) or MscI (methylation insensitive) or incubated in the absence of restriction enzyme (uncut) in the manufacturer supplied buffer (New England Biolabs, Massachusetts, USA) in a 50 µl reaction volume at 37°C for 4 h. The reaction was terminated by inactivating the enzymes at 80°C for 20 min. An aliquot of the digest (5 µl) containing 6 ng of genomic DNA was then used in qPCR using primers (SK160 and SK161, ) targeting the 5′ Moloney murine leukemia virus LTR and 5′ untranslated region (UTR) ( ). .. The PCR was carried out using SsoAdvanced SYBR Green Supermix (Bio-Rad, Hercules, CA, USA; Catalog no. 1725261) in a Bio-Rad CFX96 thermocycler.

    Pulsed-Field Gel:

    Article Title: Nisin H Is a New Nisin Variant Produced by the Gut-Derived Strain Streptococcus hyointestinalis DPC6484
    Article Snippet: .. Molecular fingerprinting of S. hyointestinalis isolates was performed by pulsed-field gel electrophoresis (PFGE) as described by Simpson et al. (2002) ( ) using SmaI restriction endonucleases and DNA molecular weight markers (9.42 to 242.50 kb; New England BioLabs, Beverly, MA). .. DNA fragments were resolved with a CHEF (contour-clamped homogeneous electric field) DRIII pulsed-field system (Bio-Rad Laboratories) at 6 V/cm for 18 h with a 1- to 30-s linear ramp time to resolve bands.

    Expressing:

    Article Title: Oncosuppressive Suicide Gene Virotherapy "PVH1-yCD/5-FC" for Pancreatic Peritoneal Carcinomatosis Treatment: NF?B and Akt/PI3K Involvement
    Article Snippet: .. Recombinant PV-H1 virus constructions for yCD and GFP expression The PV-H1-based vector DNA, phH1Δ800 , was linearized with SmaI restriction enzyme and subsequently dephosphorylated by the calf intestinal phosphatase (New England Biolabs-OZYME, Saint Quentin Yveline; France). .. The yCD gene was isolated from yeast genomic DNA (strain D4916 from Sigma-Aldrich) using specific probes: Forward: 5′-att ctcgag c gccaccatgg tgacagggggaatg-3′ containing a Kozak sequence (bold type) and XhoI restriction site (underlined), and Reverse: 5′-att ggatcc ctactcaccaatatcttcaaacc-3′ containing BamH1 restriction site (underlined), and was phosphorylated with T4 Polynucleotide Kinase (New England Biolabs-Ozyme, Montigny-Le-Bretonneux; France).

    Recombinant:

    Article Title: Oncosuppressive Suicide Gene Virotherapy "PVH1-yCD/5-FC" for Pancreatic Peritoneal Carcinomatosis Treatment: NF?B and Akt/PI3K Involvement
    Article Snippet: .. Recombinant PV-H1 virus constructions for yCD and GFP expression The PV-H1-based vector DNA, phH1Δ800 , was linearized with SmaI restriction enzyme and subsequently dephosphorylated by the calf intestinal phosphatase (New England Biolabs-OZYME, Saint Quentin Yveline; France). .. The yCD gene was isolated from yeast genomic DNA (strain D4916 from Sigma-Aldrich) using specific probes: Forward: 5′-att ctcgag c gccaccatgg tgacagggggaatg-3′ containing a Kozak sequence (bold type) and XhoI restriction site (underlined), and Reverse: 5′-att ggatcc ctactcaccaatatcttcaaacc-3′ containing BamH1 restriction site (underlined), and was phosphorylated with T4 Polynucleotide Kinase (New England Biolabs-Ozyme, Montigny-Le-Bretonneux; France).

    Molecular Weight:

    Article Title: Nisin H Is a New Nisin Variant Produced by the Gut-Derived Strain Streptococcus hyointestinalis DPC6484
    Article Snippet: .. Molecular fingerprinting of S. hyointestinalis isolates was performed by pulsed-field gel electrophoresis (PFGE) as described by Simpson et al. (2002) ( ) using SmaI restriction endonucleases and DNA molecular weight markers (9.42 to 242.50 kb; New England BioLabs, Beverly, MA). .. DNA fragments were resolved with a CHEF (contour-clamped homogeneous electric field) DRIII pulsed-field system (Bio-Rad Laboratories) at 6 V/cm for 18 h with a 1- to 30-s linear ramp time to resolve bands.

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  • 99
    New England Biolabs smai restriction endonucleases
    (A) <t>PFGE</t> macrorestriction patterns of Streptococcus hyointestinalis strains restricted with <t>SmaI.</t> Lane 1, S. hyointestinalis DPC6484; lane 2, S. hyointestinalis LMG14579; lane 3, S. hyointestinalis LMG14581; lane 4, S. hyointestinalis LMG14582; lane 5, S. hyointestinalis LMG14583; lane 6, S. hyointestinalis LMG14585; lane 7, S. hyointestinalis LMG14586; lane 8, S. hyointestinalis LMG14587. (B) (i) PCR amplification of strains of S. hyointestinalis template DNA with nshT -, nshH -, nshF -, and nshR -specific primers. Lanes correspond to those in panel A. (ii) Comparison of antimicrobial activities of S. hyointesinalis strains DPC6484 (H), LMG14579, LMG14581, LMG14582, LMG14583, LMG14585, LMG14586, and LMG14587 against the indicator strain, L. delbrueckii subsp. bulgaricus LMG6901. Wells are labeled with H (for nisin H) and with the last two digits of the strain designation for the other strains.
    Smai Restriction Endonucleases, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 97 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/smai restriction endonucleases/product/New England Biolabs
    Average 99 stars, based on 97 article reviews
    Price from $9.99 to $1999.99
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    (A) PFGE macrorestriction patterns of Streptococcus hyointestinalis strains restricted with SmaI. Lane 1, S. hyointestinalis DPC6484; lane 2, S. hyointestinalis LMG14579; lane 3, S. hyointestinalis LMG14581; lane 4, S. hyointestinalis LMG14582; lane 5, S. hyointestinalis LMG14583; lane 6, S. hyointestinalis LMG14585; lane 7, S. hyointestinalis LMG14586; lane 8, S. hyointestinalis LMG14587. (B) (i) PCR amplification of strains of S. hyointestinalis template DNA with nshT -, nshH -, nshF -, and nshR -specific primers. Lanes correspond to those in panel A. (ii) Comparison of antimicrobial activities of S. hyointesinalis strains DPC6484 (H), LMG14579, LMG14581, LMG14582, LMG14583, LMG14585, LMG14586, and LMG14587 against the indicator strain, L. delbrueckii subsp. bulgaricus LMG6901. Wells are labeled with H (for nisin H) and with the last two digits of the strain designation for the other strains.

    Journal: Applied and Environmental Microbiology

    Article Title: Nisin H Is a New Nisin Variant Produced by the Gut-Derived Strain Streptococcus hyointestinalis DPC6484

    doi: 10.1128/AEM.00212-15

    Figure Lengend Snippet: (A) PFGE macrorestriction patterns of Streptococcus hyointestinalis strains restricted with SmaI. Lane 1, S. hyointestinalis DPC6484; lane 2, S. hyointestinalis LMG14579; lane 3, S. hyointestinalis LMG14581; lane 4, S. hyointestinalis LMG14582; lane 5, S. hyointestinalis LMG14583; lane 6, S. hyointestinalis LMG14585; lane 7, S. hyointestinalis LMG14586; lane 8, S. hyointestinalis LMG14587. (B) (i) PCR amplification of strains of S. hyointestinalis template DNA with nshT -, nshH -, nshF -, and nshR -specific primers. Lanes correspond to those in panel A. (ii) Comparison of antimicrobial activities of S. hyointesinalis strains DPC6484 (H), LMG14579, LMG14581, LMG14582, LMG14583, LMG14585, LMG14586, and LMG14587 against the indicator strain, L. delbrueckii subsp. bulgaricus LMG6901. Wells are labeled with H (for nisin H) and with the last two digits of the strain designation for the other strains.

    Article Snippet: Molecular fingerprinting of S. hyointestinalis isolates was performed by pulsed-field gel electrophoresis (PFGE) as described by Simpson et al. (2002) ( ) using SmaI restriction endonucleases and DNA molecular weight markers (9.42 to 242.50 kb; New England BioLabs, Beverly, MA).

    Techniques: Polymerase Chain Reaction, Amplification, Labeling

    PFGE-based clustering of C. coli strains investigated in this study. All 68 strains were analyzed by PFGE with SmaI and KpnI, and cluster analysis of the patterns was performed by BioNumerics as described in Materials and Methods. Two strains (1004 A

    Journal: Applied and Environmental Microbiology

    Article Title: Clonal Population Structure and Specific Genotypes of Multidrug-Resistant Campylobacter coli from Turkeys ▿

    doi: 10.1128/AEM.02346-06

    Figure Lengend Snippet: PFGE-based clustering of C. coli strains investigated in this study. All 68 strains were analyzed by PFGE with SmaI and KpnI, and cluster analysis of the patterns was performed by BioNumerics as described in Materials and Methods. Two strains (1004 A

    Article Snippet: PFGE was performed using SmaI and KpnI (New England Biolabs) by following the protocol described by Ribot et al. ( ) with a few minor modifications; specifically, the prerestriction step was eliminated and the gels were electrophoresed for 22 h. TIFF images of banding patterns resulting from PFGE and fla typing were analyzed by BioNumerics (version 4.6; Applied Maths).

    Techniques:

    PFGE profiles of group I strains. Nineteen MDR strains were characterized by PFGE using SmaI and KpnI, and cluster analysis of the patterns was performed by BioNumerics as described in Materials and Methods. The line labeled “a” indicates

    Journal: Applied and Environmental Microbiology

    Article Title: Clonal Population Structure and Specific Genotypes of Multidrug-Resistant Campylobacter coli from Turkeys ▿

    doi: 10.1128/AEM.02346-06

    Figure Lengend Snippet: PFGE profiles of group I strains. Nineteen MDR strains were characterized by PFGE using SmaI and KpnI, and cluster analysis of the patterns was performed by BioNumerics as described in Materials and Methods. The line labeled “a” indicates

    Article Snippet: PFGE was performed using SmaI and KpnI (New England Biolabs) by following the protocol described by Ribot et al. ( ) with a few minor modifications; specifically, the prerestriction step was eliminated and the gels were electrophoresed for 22 h. TIFF images of banding patterns resulting from PFGE and fla typing were analyzed by BioNumerics (version 4.6; Applied Maths).

    Techniques: Labeling

    A conserved SmaI PFGE pattern (type a ) was disseminated among MDR strains of different STs.

    Journal: Applied and Environmental Microbiology

    Article Title: Clonal Population Structure and Specific Genotypes of Multidrug-Resistant Campylobacter coli from Turkeys ▿

    doi: 10.1128/AEM.02346-06

    Figure Lengend Snippet: A conserved SmaI PFGE pattern (type a ) was disseminated among MDR strains of different STs.

    Article Snippet: PFGE was performed using SmaI and KpnI (New England Biolabs) by following the protocol described by Ribot et al. ( ) with a few minor modifications; specifically, the prerestriction step was eliminated and the gels were electrophoresed for 22 h. TIFF images of banding patterns resulting from PFGE and fla typing were analyzed by BioNumerics (version 4.6; Applied Maths).

    Techniques:

    PFGE profiles of group II strains. Forty-two strains were characterized by PFGE using SmaI and KpnI, and cluster analysis of the patterns was performed by BioNumerics as described in Materials and Methods. Strains which were not resistant to all six antibiotics

    Journal: Applied and Environmental Microbiology

    Article Title: Clonal Population Structure and Specific Genotypes of Multidrug-Resistant Campylobacter coli from Turkeys ▿

    doi: 10.1128/AEM.02346-06

    Figure Lengend Snippet: PFGE profiles of group II strains. Forty-two strains were characterized by PFGE using SmaI and KpnI, and cluster analysis of the patterns was performed by BioNumerics as described in Materials and Methods. Strains which were not resistant to all six antibiotics

    Article Snippet: PFGE was performed using SmaI and KpnI (New England Biolabs) by following the protocol described by Ribot et al. ( ) with a few minor modifications; specifically, the prerestriction step was eliminated and the gels were electrophoresed for 22 h. TIFF images of banding patterns resulting from PFGE and fla typing were analyzed by BioNumerics (version 4.6; Applied Maths).

    Techniques:

    PFGE profiles of 13 strains with various STs but a conserved PFGE profile (type a ). Cluster analysis of the SmaI and KpnI patterns was performed by BioNumerics as described in Materials and Methods.

    Journal: Applied and Environmental Microbiology

    Article Title: Clonal Population Structure and Specific Genotypes of Multidrug-Resistant Campylobacter coli from Turkeys ▿

    doi: 10.1128/AEM.02346-06

    Figure Lengend Snippet: PFGE profiles of 13 strains with various STs but a conserved PFGE profile (type a ). Cluster analysis of the SmaI and KpnI patterns was performed by BioNumerics as described in Materials and Methods.

    Article Snippet: PFGE was performed using SmaI and KpnI (New England Biolabs) by following the protocol described by Ribot et al. ( ) with a few minor modifications; specifically, the prerestriction step was eliminated and the gels were electrophoresed for 22 h. TIFF images of banding patterns resulting from PFGE and fla typing were analyzed by BioNumerics (version 4.6; Applied Maths).

    Techniques:

    Alterations of restriction enzyme accessibility in the juxtaposed region of Crabp1 promoter along the course of adipocyte differentiation. ( A ) Detection of nucleosome array. Chromatin of MNase partially digested nuclei was separated on agarose gels followed by Southern blot analysis (left panel) and EtBr staining (right panel). A probe used covering the TIS (HinfI-PvuII) was used for Southern blot analysis. ( B–F ) Nuclei (differentiation Days 0, 4, 8) were digested with the indicated restriction enzymes and the recovered chromatin DNAs were completely re-digested with ApaI ( B–D ) or PstI ( E and F ) (the relationship of specific restriction site and each individual nucleosome was depicted in panel G), followed by Southern blot analyses using probe 1 ( D ) or probe 2 (B–C and E–F). ( G ) Schematic description of restriction enzyme digestion, nucleosomes, and predicted fragments detected with probes on the Southern blots. Complete digestion with ApaI produced 1.3 kb, and complete digestion with PstI produced 1.65 kb fragments. The generated fragments by digestion with XhoI, PstI, SmaI, SpeI and ApaLI for the corresponding nucleosome are 0.93, 0.67, 0.86, 0.55 and 0.65 kb, respectively. Nucleosomes on this array were named N5 to N-1, from the 5′- to the 3′-ends of this chromatin segment. Experiments were performed for least three times.

    Journal: Nucleic Acids Research

    Article Title: RIP140 in thyroid hormone-repression and chromatin remodeling of Crabp1 gene during adipocyte differentiation

    doi: 10.1093/nar/gkp780

    Figure Lengend Snippet: Alterations of restriction enzyme accessibility in the juxtaposed region of Crabp1 promoter along the course of adipocyte differentiation. ( A ) Detection of nucleosome array. Chromatin of MNase partially digested nuclei was separated on agarose gels followed by Southern blot analysis (left panel) and EtBr staining (right panel). A probe used covering the TIS (HinfI-PvuII) was used for Southern blot analysis. ( B–F ) Nuclei (differentiation Days 0, 4, 8) were digested with the indicated restriction enzymes and the recovered chromatin DNAs were completely re-digested with ApaI ( B–D ) or PstI ( E and F ) (the relationship of specific restriction site and each individual nucleosome was depicted in panel G), followed by Southern blot analyses using probe 1 ( D ) or probe 2 (B–C and E–F). ( G ) Schematic description of restriction enzyme digestion, nucleosomes, and predicted fragments detected with probes on the Southern blots. Complete digestion with ApaI produced 1.3 kb, and complete digestion with PstI produced 1.65 kb fragments. The generated fragments by digestion with XhoI, PstI, SmaI, SpeI and ApaLI for the corresponding nucleosome are 0.93, 0.67, 0.86, 0.55 and 0.65 kb, respectively. Nucleosomes on this array were named N5 to N-1, from the 5′- to the 3′-ends of this chromatin segment. Experiments were performed for least three times.

    Article Snippet: Isolated nuclei from differentiating 3T3-L1 cells were digested with 100 U of PstI, XhoI, SmaI, SpeI and ApaLI (New England Biolabs) for 30 min.

    Techniques: Southern Blot, Staining, Produced, Generated

    Schematic representation of recombinant parvoviral vector constructions. The upper diagram depicts the empty vector DNA clone PVH1-Δ800 which has 800-bp deletion in the VP coding region and carries a multiple cloning sequence (MluI/SmaI polylinker) at the VP2 translation initiation site. DNA inserts encoding for GFP or catalytic yCD were introduced in polylinker using XhoI and BamH1 restriction enzymes. The resulting rPVH1-GFP and rPVH1-yCD plasmids were used for the production of corresponding non-replicative recombinant parvoviruses.

    Journal: PLoS ONE

    Article Title: Oncosuppressive Suicide Gene Virotherapy "PVH1-yCD/5-FC" for Pancreatic Peritoneal Carcinomatosis Treatment: NF?B and Akt/PI3K Involvement

    doi: 10.1371/journal.pone.0070594

    Figure Lengend Snippet: Schematic representation of recombinant parvoviral vector constructions. The upper diagram depicts the empty vector DNA clone PVH1-Δ800 which has 800-bp deletion in the VP coding region and carries a multiple cloning sequence (MluI/SmaI polylinker) at the VP2 translation initiation site. DNA inserts encoding for GFP or catalytic yCD were introduced in polylinker using XhoI and BamH1 restriction enzymes. The resulting rPVH1-GFP and rPVH1-yCD plasmids were used for the production of corresponding non-replicative recombinant parvoviruses.

    Article Snippet: Recombinant PV-H1 virus constructions for yCD and GFP expression The PV-H1-based vector DNA, phH1Δ800 , was linearized with SmaI restriction enzyme and subsequently dephosphorylated by the calf intestinal phosphatase (New England Biolabs-OZYME, Saint Quentin Yveline; France).

    Techniques: Recombinant, Plasmid Preparation, Clone Assay, Sequencing