r0141  (New England Biolabs)


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    Name:
    SmaI
    Description:
    SmaI 10 000 units
    Catalog Number:
    R0141L
    Price:
    261
    Category:
    Restriction Enzymes
    Size:
    10 000 units
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    Structured Review

    New England Biolabs r0141
    SmaI
    SmaI 10 000 units
    https://www.bioz.com/result/r0141/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    r0141 - by Bioz Stars, 2021-05
    86/100 stars

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    Related Articles

    Sequencing:

    Article Title: High-Temperature Ethanol Fermentation and Transformation with Linear DNA in the Thermotolerant Yeast Kluyveromyces marxianus DMKU3-1042 ▿
    Article Snippet: The mixture was vortexed for 30 s, incubated at 42°C for 40 min, and spread on uracil dropout plates. .. S. cerevisiae centromere/autonomously replicating sequence (ARS) plasmid pRS316 ( ) containing Sc URA3 as a marker was used for K. marxianus transformations. pRS316 (4.8 kb) was linearized by digestion with SmaI (New England Biolabs, MA). .. Chromosomal DNA from BY4704, K. marxianus DMKU3-1042, a K. marxianus ura3 mutant (RAK3605), and Sc URA3 transformants of the ura3 mutant was isolated and digested with BamHI (Roche Diagnostics GmbH, Mannheim, Germany).

    Plasmid Preparation:

    Article Title: High-Temperature Ethanol Fermentation and Transformation with Linear DNA in the Thermotolerant Yeast Kluyveromyces marxianus DMKU3-1042 ▿
    Article Snippet: The mixture was vortexed for 30 s, incubated at 42°C for 40 min, and spread on uracil dropout plates. .. S. cerevisiae centromere/autonomously replicating sequence (ARS) plasmid pRS316 ( ) containing Sc URA3 as a marker was used for K. marxianus transformations. pRS316 (4.8 kb) was linearized by digestion with SmaI (New England Biolabs, MA). .. Chromosomal DNA from BY4704, K. marxianus DMKU3-1042, a K. marxianus ura3 mutant (RAK3605), and Sc URA3 transformants of the ura3 mutant was isolated and digested with BamHI (Roche Diagnostics GmbH, Mannheim, Germany).

    Marker:

    Article Title: High-Temperature Ethanol Fermentation and Transformation with Linear DNA in the Thermotolerant Yeast Kluyveromyces marxianus DMKU3-1042 ▿
    Article Snippet: The mixture was vortexed for 30 s, incubated at 42°C for 40 min, and spread on uracil dropout plates. .. S. cerevisiae centromere/autonomously replicating sequence (ARS) plasmid pRS316 ( ) containing Sc URA3 as a marker was used for K. marxianus transformations. pRS316 (4.8 kb) was linearized by digestion with SmaI (New England Biolabs, MA). .. Chromosomal DNA from BY4704, K. marxianus DMKU3-1042, a K. marxianus ura3 mutant (RAK3605), and Sc URA3 transformants of the ura3 mutant was isolated and digested with BamHI (Roche Diagnostics GmbH, Mannheim, Germany).

    other:

    Article Title: An efficient method for the construction of artificial, concatemeric DNA, RNA and proteins with genetically programmed functions, using a novel, vector-enzymatic DNA fragment amplification-expression technology
    Article Snippet: Media and reagentsREases SapI, BspQI, SmaI were from New England Biolabs (Ipswich, MA, USA).

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  • 99
    New England Biolabs smai
    A series of pAMP DNA vectors. The amplification modules differ in their possibility to manipulate three reading frames, as well as the presence or absence of a His6 tag. Optionally, the modules also allow for cloning into the <t>SmaI</t> site, although <t>SapI</t> is preferred. DNA sequences of the vectors have been deposited in the GeneBank database: pAMP1-A (MK651654), pAMP1-B (MK606505), pAMP1-C (MK606506), pAMP1-HisA (MK606507), pMAP1- HisB (MK606519), pAMP1-His C (MK606520).
    Smai, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/smai/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    smai - by Bioz Stars, 2021-05
    99/100 stars
      Buy from Supplier

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    A series of pAMP DNA vectors. The amplification modules differ in their possibility to manipulate three reading frames, as well as the presence or absence of a His6 tag. Optionally, the modules also allow for cloning into the SmaI site, although SapI is preferred. DNA sequences of the vectors have been deposited in the GeneBank database: pAMP1-A (MK651654), pAMP1-B (MK606505), pAMP1-C (MK606506), pAMP1-HisA (MK606507), pMAP1- HisB (MK606519), pAMP1-His C (MK606520).

    Journal: MethodsX

    Article Title: An efficient method for the construction of artificial, concatemeric DNA, RNA and proteins with genetically programmed functions, using a novel, vector-enzymatic DNA fragment amplification-expression technology

    doi: 10.1016/j.mex.2020.101070

    Figure Lengend Snippet: A series of pAMP DNA vectors. The amplification modules differ in their possibility to manipulate three reading frames, as well as the presence or absence of a His6 tag. Optionally, the modules also allow for cloning into the SmaI site, although SapI is preferred. DNA sequences of the vectors have been deposited in the GeneBank database: pAMP1-A (MK651654), pAMP1-B (MK606505), pAMP1-C (MK606506), pAMP1-HisA (MK606507), pMAP1- HisB (MK606519), pAMP1-His C (MK606520).

    Article Snippet: Media and reagentsREases SapI, BspQI, SmaI were from New England Biolabs (Ipswich, MA, USA).

    Techniques: Amplification, Clone Assay

    Selection for loss of antibiotic resistance in ST59/SCC mec V (5C2 5) MRSA strain PM1. (A) Cartoon representation of MES structures in PM1 and the generated colonies PM1-1 to PM1-5. (B) SmaI-digested PFGE of PM1 and PM1-1 to PM1-5 and the Southern blot. The SmaI-digested DNA separated by PFGE is shown in the left. The DNA was transferred to a nylon membrane and detected by Southern blot hybridization with DIG-labeled cat -, ermB - and IS 1216V -specific probes, which are shown on the right. Abbreviations: E r , erythromycin resistant; K r , kanamycin resistant; S r , streptomycin resistant; C r , chloramphenicol resistant.

    Journal: PLoS ONE

    Article Title: Molecular Evolutionary Pathways toward Two Successful Community-Associated but Multidrug-Resistant ST59 Methicillin-Resistant Staphylococcus aureus Lineages in Taiwan: Dynamic Modes of Mobile Genetic Element Salvages

    doi: 10.1371/journal.pone.0162526

    Figure Lengend Snippet: Selection for loss of antibiotic resistance in ST59/SCC mec V (5C2 5) MRSA strain PM1. (A) Cartoon representation of MES structures in PM1 and the generated colonies PM1-1 to PM1-5. (B) SmaI-digested PFGE of PM1 and PM1-1 to PM1-5 and the Southern blot. The SmaI-digested DNA separated by PFGE is shown in the left. The DNA was transferred to a nylon membrane and detected by Southern blot hybridization with DIG-labeled cat -, ermB - and IS 1216V -specific probes, which are shown on the right. Abbreviations: E r , erythromycin resistant; K r , kanamycin resistant; S r , streptomycin resistant; C r , chloramphenicol resistant.

    Article Snippet: In brief, The DNAs of PM1 and PM1-1 to PM1-5 were digested with SmaI (New England BioLabs, Ipswich, MA) and then were separated using a CHEF-DRIII apparatus (Bio-Rad Laboratories).

    Techniques: Selection, Generated, Southern Blot, Hybridization, Labeling

    (a) Pulsed-field gel electrophoresis (PFGE) patterns of the SmaI-digested genomic DNA obtained from CA-MRSA isolates in Uruguay. Lane 1, pulsotype A (strain IH 23); lane 2, pulsotype B (strain IH 48); lane 3, pulsotype C (strain IH 46); lane 4, pulsotype D (strain IH 7); lane 5, pulsotype E (strain IH 22) and lane 6 pulsotype F (strain IH 69). (b) Pulsed-field gel electrophoresis (PFGE) patterns of the SmaI-digested genomic DNA obtained from CA-MRSA isolates in Uruguay. Lane 1, pulsotype A1 (strain IH 36); lane 2, pulsotype A (strain IH 44); lane 3, pulsotype A2 (strain IH 9).

    Journal: International Journal of Microbiology

    Article Title: Characteristics of Community-Associated Methicillin-Resistant Staphylococcus aureus (CA-MRSA) Strains Isolated from Skin and Soft-Tissue Infections in Uruguay

    doi: 10.1155/2009/472126

    Figure Lengend Snippet: (a) Pulsed-field gel electrophoresis (PFGE) patterns of the SmaI-digested genomic DNA obtained from CA-MRSA isolates in Uruguay. Lane 1, pulsotype A (strain IH 23); lane 2, pulsotype B (strain IH 48); lane 3, pulsotype C (strain IH 46); lane 4, pulsotype D (strain IH 7); lane 5, pulsotype E (strain IH 22) and lane 6 pulsotype F (strain IH 69). (b) Pulsed-field gel electrophoresis (PFGE) patterns of the SmaI-digested genomic DNA obtained from CA-MRSA isolates in Uruguay. Lane 1, pulsotype A1 (strain IH 36); lane 2, pulsotype A (strain IH 44); lane 3, pulsotype A2 (strain IH 9).

    Article Snippet: DNA was digested with SmaI (New England Biolabs).

    Techniques: Pulsed-Field Gel, Electrophoresis

    Dendrogram produced by BioNumerics software, showing distances calculated by Dice similarity index of SmaI-digested DNA fragments among 34 fusB- carrying S. epidermidis isolates. The degree of similarity is shown in the scale. Different degrees of gray

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Identification of fusB-Mediated Fusidic Acid Resistance Islands in Staphylococcus epidermidis Isolates ▿

    doi: 10.1128/AAC.00592-11

    Figure Lengend Snippet: Dendrogram produced by BioNumerics software, showing distances calculated by Dice similarity index of SmaI-digested DNA fragments among 34 fusB- carrying S. epidermidis isolates. The degree of similarity is shown in the scale. Different degrees of gray

    Article Snippet: The DNA was digested with SmaI (New England BioLabs, Ipswich, MA) and then separated in a CHEF-DRII apparatus (Bio-Rad Laboratories).

    Techniques: Produced, Software