sali treatment (New England Biolabs)
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Name:
SalI
Description:
SalI 10 000 units
Catalog Number:
r0138l
Price:
261
Size:
10 000 units
Category:
Restriction Enzymes
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Structured Review
New England Biolabs
sali treatment
SalI 10 000 units
https://www.bioz.com/result/sali treatment/product/New England Biolabs
Average 94 stars, based on 19442 article reviews
Price from $9.99 to $1999.99
SalI 10 000 units
https://www.bioz.com/result/sali treatment/product/New England Biolabs
Average 94 stars, based on 19442 article reviews
Price from $9.99 to $1999.99
sali treatment - by Bioz Stars,
2021-02
94/100 stars
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Images
1) Product Images from "Single-molecule analysis of ϕC31 integrase-mediated site-specific recombination by tethered particle motion"
Article Title: Single-molecule analysis of ϕC31 integrase-mediated site-specific recombination by tethered particle motion
Journal: Nucleic Acids Research
doi: 10.1093/nar/gkw861
Figure Legend Snippet: Bead separation from the tethered DNA substrate containing attB-attP by addition of SalI. ( A ) In the schematic representation of the 1303 bp recombination substrates (containing attB-attP or attL-attR ), the integrase cleavage sites are indicated by the short vertical arrows. The vertical line at the left and the sphere at the right indicate the tethering glass surface and the polystyrene bead, respectively. Double strand cleavage at one or both att sites will result in bead detachment from the tethered DNA. The number of molecules with detached beads following SDS treatment from three assays are tabulated. The orientation of the att sites in a substrate is indicated by the arrows. The attB-attP reactions contained integrase. The attL-attR reaction contained integrase plus gp3. ( B ) In the 1303 bp DNA substrate with head-to-tail attB-attP sites, the SalI recognition site located 241 bp away from the end attached to the glass surface. SalI digestion would release the bead along with the long DNA fragment from the short fragment that remains tethered. The BM amplitude time trace of a molecule before and after SalI addition (indicated by the short vertical arrow) are shown. The abrupt termination of the trace resulted from bead detachment. Such events from three separate experiments are tabulated. N refers to the number of DNA molecules observed.
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Related Articles
DNA Extraction:Article Title: CRISPR-Cas9-guided Genome Engineering in C. elegans Article Snippet: .. Kpn I (NEB R0142S) Sal I (NEB R0138S) Clone Assay:Article Title: Self-reactive IgE exacerbates interferon responses associated with autoimmunity Article Snippet: .. To generate the human dsDNA-specific IgE (IgED ), E11 variable regions were cloned by restriction digestion using BssHII and Article Title: MiR-891a-5p as a prognostic marker and therapeutic target for hormone receptor-positive breast cancer Article Snippet: .. Dual-Luciferase Reporter Assay To generate the pmirGLO-ADAM10-3'UTR reporter plasmid, the human ADAM10-3'UTR region (423 bp) encompassing the binding site of miR-891a-5pwas amplified using PCR primers (3'UTR-F: 5'-GCAGCTAGCACTAAACCCTCACAAG-3'; 3'UTR -R: 5'-TTGCGTCGACACAGAAGTACAGTGTA-3') and then cloned into the luciferase vector pmirGLO(Promega) using NheI and Amplification:Article Title: Enhanced Control of Oncolytic Measles Virus Using MicroRNA Target Sites Article Snippet: .. Finally, the modified N gene was excised with MluI and SbfI and inserted into the pMV-EGFP vector digested with MluI and SbfI to generate pMV-EGFP-NmiRTS7 . pMV-EGFP-FmiRTS7 and -HmiRTS7 were generated by insertion of custom-designed, complementary DNA oligonucleotides encoding the miRTS box for miR-7-5p (with compatible ends) into the 3′ UTR of F via PacI or of H via SpeI as described before., To generate pMV-EGFP-LmiRTS7 , the 3′ portion of the L gene, including its 3′ UTR, was amplified by high-fidelity PCR (Phusion polymerase, New England Biolabs, Frankfurt am Main, Germany) using primers that introduce artificial BamHI and Article Title: MiR-891a-5p as a prognostic marker and therapeutic target for hormone receptor-positive breast cancer Article Snippet: .. Dual-Luciferase Reporter Assay To generate the pmirGLO-ADAM10-3'UTR reporter plasmid, the human ADAM10-3'UTR region (423 bp) encompassing the binding site of miR-891a-5pwas amplified using PCR primers (3'UTR-F: 5'-GCAGCTAGCACTAAACCCTCACAAG-3'; 3'UTR -R: 5'-TTGCGTCGACACAGAAGTACAGTGTA-3') and then cloned into the luciferase vector pmirGLO(Promega) using NheI and Article Title: Specific disulfide cross-linking to constrict the mobile carrier domain of nonribosomal peptide synthetases Article Snippet: .. After amplification, the PCR product was digested with PciI and Reporter Assay:Article Title: MiR-891a-5p as a prognostic marker and therapeutic target for hormone receptor-positive breast cancer Article Snippet: .. Dual-Luciferase Reporter Assay To generate the pmirGLO-ADAM10-3'UTR reporter plasmid, the human ADAM10-3'UTR region (423 bp) encompassing the binding site of miR-891a-5pwas amplified using PCR primers (3'UTR-F: 5'-GCAGCTAGCACTAAACCCTCACAAG-3'; 3'UTR -R: 5'-TTGCGTCGACACAGAAGTACAGTGTA-3') and then cloned into the luciferase vector pmirGLO(Promega) using NheI and Concentration Assay:Article Title: CRISPR-Cas9-guided Genome Engineering in C. elegans Article Snippet: .. Kpn I (NEB R0142S) Sal I (NEB R0138S) Mutagenesis:Article Title: CRISPR-Cas9-guided Genome Engineering in C. elegans Article Snippet: .. Kpn I (NEB R0142S) Sal I (NEB R0138S) Subcloning:Article Title: Enhanced Control of Oncolytic Measles Virus Using MicroRNA Target Sites Article Snippet: .. Finally, the modified N gene was excised with MluI and SbfI and inserted into the pMV-EGFP vector digested with MluI and SbfI to generate pMV-EGFP-NmiRTS7 . pMV-EGFP-FmiRTS7 and -HmiRTS7 were generated by insertion of custom-designed, complementary DNA oligonucleotides encoding the miRTS box for miR-7-5p (with compatible ends) into the 3′ UTR of F via PacI or of H via SpeI as described before., To generate pMV-EGFP-LmiRTS7 , the 3′ portion of the L gene, including its 3′ UTR, was amplified by high-fidelity PCR (Phusion polymerase, New England Biolabs, Frankfurt am Main, Germany) using primers that introduce artificial BamHI and Spectrophotometry:Article Title: CRISPR-Cas9-guided Genome Engineering in C. elegans Article Snippet: .. Kpn I (NEB R0142S) Sal I (NEB R0138S) Electroporation Bacterial Transformation:Article Title: CRISPR-Cas9-guided Genome Engineering in C. elegans Article Snippet: .. Kpn I (NEB R0142S) Sal I (NEB R0138S) Polymerase Chain Reaction:Article Title: CRISPR-Cas9-guided Genome Engineering in C. elegans Article Snippet: .. Kpn I (NEB R0142S) Sal I (NEB R0138S) Article Title: Multiple domains of EBER 1, an Epstein-Barr virus noncoding RNA, recruit human ribosomal protein L22 Article Snippet: .. The resulting PCR product was restriction digested with EcoRI and Article Title: Enhanced Control of Oncolytic Measles Virus Using MicroRNA Target Sites Article Snippet: .. Finally, the modified N gene was excised with MluI and SbfI and inserted into the pMV-EGFP vector digested with MluI and SbfI to generate pMV-EGFP-NmiRTS7 . pMV-EGFP-FmiRTS7 and -HmiRTS7 were generated by insertion of custom-designed, complementary DNA oligonucleotides encoding the miRTS box for miR-7-5p (with compatible ends) into the 3′ UTR of F via PacI or of H via SpeI as described before., To generate pMV-EGFP-LmiRTS7 , the 3′ portion of the L gene, including its 3′ UTR, was amplified by high-fidelity PCR (Phusion polymerase, New England Biolabs, Frankfurt am Main, Germany) using primers that introduce artificial BamHI and Article Title: MiR-891a-5p as a prognostic marker and therapeutic target for hormone receptor-positive breast cancer Article Snippet: .. Dual-Luciferase Reporter Assay To generate the pmirGLO-ADAM10-3'UTR reporter plasmid, the human ADAM10-3'UTR region (423 bp) encompassing the binding site of miR-891a-5pwas amplified using PCR primers (3'UTR-F: 5'-GCAGCTAGCACTAAACCCTCACAAG-3'; 3'UTR -R: 5'-TTGCGTCGACACAGAAGTACAGTGTA-3') and then cloned into the luciferase vector pmirGLO(Promega) using NheI and Article Title: Specific disulfide cross-linking to constrict the mobile carrier domain of nonribosomal peptide synthetases Article Snippet: .. After amplification, the PCR product was digested with PciI and Article Title: Profound analgesia is associated with a truncated peptide resulting from tissue specific alternative splicing of DRG CA8-204 regulated by an exon-level cis-eQTL Article Snippet: .. The Generated:Article Title: Enhanced Control of Oncolytic Measles Virus Using MicroRNA Target Sites Article Snippet: .. Finally, the modified N gene was excised with MluI and SbfI and inserted into the pMV-EGFP vector digested with MluI and SbfI to generate pMV-EGFP-NmiRTS7 . pMV-EGFP-FmiRTS7 and -HmiRTS7 were generated by insertion of custom-designed, complementary DNA oligonucleotides encoding the miRTS box for miR-7-5p (with compatible ends) into the 3′ UTR of F via PacI or of H via SpeI as described before., To generate pMV-EGFP-LmiRTS7 , the 3′ portion of the L gene, including its 3′ UTR, was amplified by high-fidelity PCR (Phusion polymerase, New England Biolabs, Frankfurt am Main, Germany) using primers that introduce artificial BamHI and Luciferase:Article Title: MiR-891a-5p as a prognostic marker and therapeutic target for hormone receptor-positive breast cancer Article Snippet: .. Dual-Luciferase Reporter Assay To generate the pmirGLO-ADAM10-3'UTR reporter plasmid, the human ADAM10-3'UTR region (423 bp) encompassing the binding site of miR-891a-5pwas amplified using PCR primers (3'UTR-F: 5'-GCAGCTAGCACTAAACCCTCACAAG-3'; 3'UTR -R: 5'-TTGCGTCGACACAGAAGTACAGTGTA-3') and then cloned into the luciferase vector pmirGLO(Promega) using NheI and Introduce:Article Title: Enhanced Control of Oncolytic Measles Virus Using MicroRNA Target Sites Article Snippet: .. Finally, the modified N gene was excised with MluI and SbfI and inserted into the pMV-EGFP vector digested with MluI and SbfI to generate pMV-EGFP-NmiRTS7 . pMV-EGFP-FmiRTS7 and -HmiRTS7 were generated by insertion of custom-designed, complementary DNA oligonucleotides encoding the miRTS box for miR-7-5p (with compatible ends) into the 3′ UTR of F via PacI or of H via SpeI as described before., To generate pMV-EGFP-LmiRTS7 , the 3′ portion of the L gene, including its 3′ UTR, was amplified by high-fidelity PCR (Phusion polymerase, New England Biolabs, Frankfurt am Main, Germany) using primers that introduce artificial BamHI and Expressing:Article Title: Self-reactive IgE exacerbates interferon responses associated with autoimmunity Article Snippet: .. To generate the human dsDNA-specific IgE (IgED ), E11 variable regions were cloned by restriction digestion using BssHII and Modification:Article Title: Enhanced Control of Oncolytic Measles Virus Using MicroRNA Target Sites Article Snippet: .. Finally, the modified N gene was excised with MluI and SbfI and inserted into the pMV-EGFP vector digested with MluI and SbfI to generate pMV-EGFP-NmiRTS7 . pMV-EGFP-FmiRTS7 and -HmiRTS7 were generated by insertion of custom-designed, complementary DNA oligonucleotides encoding the miRTS box for miR-7-5p (with compatible ends) into the 3′ UTR of F via PacI or of H via SpeI as described before., To generate pMV-EGFP-LmiRTS7 , the 3′ portion of the L gene, including its 3′ UTR, was amplified by high-fidelity PCR (Phusion polymerase, New England Biolabs, Frankfurt am Main, Germany) using primers that introduce artificial BamHI and Binding Assay:Article Title: MiR-891a-5p as a prognostic marker and therapeutic target for hormone receptor-positive breast cancer Article Snippet: .. Dual-Luciferase Reporter Assay To generate the pmirGLO-ADAM10-3'UTR reporter plasmid, the human ADAM10-3'UTR region (423 bp) encompassing the binding site of miR-891a-5pwas amplified using PCR primers (3'UTR-F: 5'-GCAGCTAGCACTAAACCCTCACAAG-3'; 3'UTR -R: 5'-TTGCGTCGACACAGAAGTACAGTGTA-3') and then cloned into the luciferase vector pmirGLO(Promega) using NheI and other:Article Title: Single-molecule analysis of ϕC31 integrase-mediated site-specific recombination by tethered particle motion Article Snippet: SalI treatment of tethered DNA molecules The DNA molecules were tethered to the glass surface in the reaction chamber containing the Plasmid Preparation:Article Title: CRISPR-Cas9-guided Genome Engineering in C. elegans Article Snippet: .. Kpn I (NEB R0142S) Sal I (NEB R0138S) Article Title: Multiple domains of EBER 1, an Epstein-Barr virus noncoding RNA, recruit human ribosomal protein L22 Article Snippet: .. The resulting PCR product was restriction digested with EcoRI and Article Title: Self-reactive IgE exacerbates interferon responses associated with autoimmunity Article Snippet: .. To generate the human dsDNA-specific IgE (IgED ), E11 variable regions were cloned by restriction digestion using BssHII and Article Title: Enhanced Control of Oncolytic Measles Virus Using MicroRNA Target Sites Article Snippet: .. Finally, the modified N gene was excised with MluI and SbfI and inserted into the pMV-EGFP vector digested with MluI and SbfI to generate pMV-EGFP-NmiRTS7 . pMV-EGFP-FmiRTS7 and -HmiRTS7 were generated by insertion of custom-designed, complementary DNA oligonucleotides encoding the miRTS box for miR-7-5p (with compatible ends) into the 3′ UTR of F via PacI or of H via SpeI as described before., To generate pMV-EGFP-LmiRTS7 , the 3′ portion of the L gene, including its 3′ UTR, was amplified by high-fidelity PCR (Phusion polymerase, New England Biolabs, Frankfurt am Main, Germany) using primers that introduce artificial BamHI and Article Title: MiR-891a-5p as a prognostic marker and therapeutic target for hormone receptor-positive breast cancer Article Snippet: .. Dual-Luciferase Reporter Assay To generate the pmirGLO-ADAM10-3'UTR reporter plasmid, the human ADAM10-3'UTR region (423 bp) encompassing the binding site of miR-891a-5pwas amplified using PCR primers (3'UTR-F: 5'-GCAGCTAGCACTAAACCCTCACAAG-3'; 3'UTR -R: 5'-TTGCGTCGACACAGAAGTACAGTGTA-3') and then cloned into the luciferase vector pmirGLO(Promega) using NheI and Article Title: Specific disulfide cross-linking to constrict the mobile carrier domain of nonribosomal peptide synthetases Article Snippet: .. After amplification, the PCR product was digested with PciI and Article Title: Profound analgesia is associated with a truncated peptide resulting from tissue specific alternative splicing of DRG CA8-204 regulated by an exon-level cis-eQTL Article Snippet: .. The |