eco53ki  (New England Biolabs)


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    Name:
    Eco53kI
    Description:

    Catalog Number:
    R0116
    Price:
    62
    Category:
    Restriction Enzymes
    Applications:
    DNA Manipulation
    Size:
    1000 units
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    Structured Review

    New England Biolabs eco53ki
    Eco53kI

    https://www.bioz.com/result/eco53ki/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    eco53ki - by Bioz Stars, 2021-08
    94/100 stars

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    Related Articles

    Whole Genome Amplification:

    Article Title: Characterization, Mapping, and Distribution of the Two XMRV Parental Proviruses
    Article Snippet: .. Mouse genomic DNA (15 μg) either was directly digested with BsmI or Eco53KI (NEB) or was first obtained by whole-genome amplification from much smaller quantities using the REPLI-g midikit (Qiagen) and phenol extraction. ..

    Ligation:

    Article Title: Generation and characterization of novel recombinant anti-hERG1 scFv antibodies for cancer molecular imaging
    Article Snippet: .. The DNA was then cut with FspI and AvrII to allow its ligation into the pPIC9K vector that had been cut with Eco53KI and AvrII restriction enzymes (New England BioLabs, Massachusetts, USA). ..

    Plasmid Preparation:

    Article Title: Generation and characterization of novel recombinant anti-hERG1 scFv antibodies for cancer molecular imaging
    Article Snippet: .. The DNA was then cut with FspI and AvrII to allow its ligation into the pPIC9K vector that had been cut with Eco53KI and AvrII restriction enzymes (New England BioLabs, Massachusetts, USA). ..

    Article Title: Motif-driven interactions between RNA and PRC2 are rheostats that regulate transcription elongation
    Article Snippet: .. Then, 2,271 kb ScaI – XmnI fragment of cloned sequence was introduced into Eco53KI – digested pMB1610-pRR-Puro CRISPR reporter plasmid. ..

    Article Title: Subdomain cryo-EM structure of nodaviral replication protein A crown complex provides mechanistic insights into RNA genome replication
    Article Snippet: .. To generate the E. coli expression vector for GFP1-10, pIE1-GFP1-10 was digested with EcoRV and Eco53kI and the resulting GFP1-10 fragment was inserted into an XmnI digested pMAL-c5x (New England Biolabs) vector. ..

    Article Title: Enzymatic and spectroscopic properties of a thermostable [NiFe]-hydrogenase performing H2-driven NAD+-reduction in the presence of O2
    Article Snippet: .. A PSH - hox Strep FUHYW fragment was cut out with Eco53KI and XbaI, and the resulting 5.7 kb fragment was inserted into the ScaI-XbaI-cut vector pEDY309 [ ]. ..

    Clone Assay:

    Article Title: Motif-driven interactions between RNA and PRC2 are rheostats that regulate transcription elongation
    Article Snippet: .. Then, 2,271 kb ScaI – XmnI fragment of cloned sequence was introduced into Eco53KI – digested pMB1610-pRR-Puro CRISPR reporter plasmid. ..

    Sequencing:

    Article Title: Motif-driven interactions between RNA and PRC2 are rheostats that regulate transcription elongation
    Article Snippet: .. Then, 2,271 kb ScaI – XmnI fragment of cloned sequence was introduced into Eco53KI – digested pMB1610-pRR-Puro CRISPR reporter plasmid. ..

    CRISPR:

    Article Title: Motif-driven interactions between RNA and PRC2 are rheostats that regulate transcription elongation
    Article Snippet: .. Then, 2,271 kb ScaI – XmnI fragment of cloned sequence was introduced into Eco53KI – digested pMB1610-pRR-Puro CRISPR reporter plasmid. ..

    Expressing:

    Article Title: Subdomain cryo-EM structure of nodaviral replication protein A crown complex provides mechanistic insights into RNA genome replication
    Article Snippet: .. To generate the E. coli expression vector for GFP1-10, pIE1-GFP1-10 was digested with EcoRV and Eco53kI and the resulting GFP1-10 fragment was inserted into an XmnI digested pMAL-c5x (New England Biolabs) vector. ..

    other:

    Article Title: Local auxin competition explains fragmented differentiation patterns
    Article Snippet: The restriction sites that were used were KpnI or Eco53kI for the forward primers and SbfI for the reverse primers (Supplementary Table ).

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  • 94
    New England Biolabs eco53ki
    Eco53ki, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/eco53ki/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    eco53ki - by Bioz Stars, 2021-08
    94/100 stars
      Buy from Supplier

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