bstxi (New England Biolabs)
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Name:
BstXI
Description:
BstXI 5 000 units
Catalog Number:
r0113l
Price:
282
Category:
Restriction Enzymes
Size:
5 000 units
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Structured Review
New England Biolabs
bstxi
BstXI 5 000 units
https://www.bioz.com/result/bstxi/product/New England Biolabs
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
BstXI 5 000 units
https://www.bioz.com/result/bstxi/product/New England Biolabs
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
bstxi - by Bioz Stars,
2021-03
94/100 stars
Images
1) Product Images from "Cross-Link Structure Affects Replication-Independent DNA Interstrand Cross-Link Repair in Mammalian Cells †"
Article Title: Cross-Link Structure Affects Replication-Independent DNA Interstrand Cross-Link Repair in Mammalian Cells †
Journal: Biochemistry
doi: 10.1021/bi902169q
Figure Legend Snippet: A) Scheme of cross-linked plasmid preparation. First, p3CMV was double digested with Van91I and BstXI to create the linear duplex, 2A.1 . Next, an adaptor duplex was ligated onto the BstXI compatible end followed by ligation with the cross-linked duplex to form 2A.2 . Blocking one end of the duplex prevented multiple ligations of the cross-linked duplex. Digestion by BstXI released the adaptor duplex to produce the cross-linked linear duplex, 2A.3 . Finally, the linear cross-linked duplex was circularized under dilute conditions to form the single site-specific interstrand cross-linked plasmid, p3CMV-X . B) Characterization of interstrand cross-linked plasmids. Plasmids were digested with a restriction enzyme to release a 150bp fragment and the fragments were radiolabeled using the Klenow fragment of E. coli DNA polymerase I. The labeled fragments were then analyzed on a 6% gel under denaturing conditions.
Techniques Used: Plasmid Preparation, Ligation, Blocking Assay, Labeling
2) Product Images from "Sirtuin 1 Is a Key Regulator of the Interleukin-12 p70/Interleukin-23 Balance in Human Dendritic Cells *"
Article Title: Sirtuin 1 Is a Key Regulator of the Interleukin-12 p70/Interleukin-23 Balance in Human Dendritic Cells *
Journal: The Journal of Biological Chemistry
doi: 10.1074/jbc.M112.391839
Figure Legend Snippet: Accessibility of Nuc-1 and Nuc-2 of the il12a promoter. Accessibility was assayed by CHART-PCR. Nuclei were digested with 50 units BstXI or 50 units PshAI for 1 h at 37 °C, and genomic DNA was used to perform SYBR Green quantitative PCR. Amplification with primers for the Nuc-1-encompassing PshAI site is sensitive to remodeling of Nuc-1, and amplification with primers for Nuc-2 encompassing BstXI is sensitive to remodeling of Nuc-2. Results are expressed as a percentage of the undigested sample for each cell treatment as described under “Experimental Procedures.” Results represent the mean ± S.D. of five experiments with triplicate samples. *, p
Techniques Used: Polymerase Chain Reaction, SYBR Green Assay, Real-time Polymerase Chain Reaction, Amplification
Related Articles
Polymerase Chain Reaction:Article Title: A novel Gateway®-compatible binary vector allows direct selection of recombinant clones in Agrobacterium tumefaciens Article Snippet: The iProof ™ high fidelity Taq DNA polymerase (Bio-Rad, Hercules, CA) PCR program consisted of 1 cycle at 98°C (2 min), followed by 30 cycles at 98°C (30 s), 55°C (45 s), and 72°C (1 min), and finished with a 1 cycle extension at 72°C (7 min). .. The PCR products were digested with Bbv CI and Article Title: Dynamic protein quantitation (DyProQ) of procollagen-I by CRISPR-Cas9 NanoLuciferase tagging Article Snippet: Generation of Split GFP Expressing Stable Cells To allow detection of CRISPR edited cells we included a split GFP tag developed in the Bo Huang lab . .. The sfGFP1-10 barrel was synthesised and cloned into a lentiviral vector (Vectorbuilder), and further subcloned into a CMV driven vector (pLenti CMV V5-LUC Blast (w567-1) was a gift from Eric Campeau (Addgene plasmid #21474 ; http://n2t.net/addgene:21474 ; RRID:Addgene_21474 )), briefly FLuc was removed from the vector by digesting with Article Title: A Potential Role for CHH DNA Methylation in Cotton Fiber Growth Patterns Article Snippet: Methylation-sensitive Endonuclease Digestion Genomic DNA was isolated from 7-dpa cotton ovules harvested at different times of the year. .. Aliquots (1 µg) were digested by incubation with 1 µl Bsl I (10,000 U/ml) in NEBuffer 3 at 55°C for 16 h, with 1 µl HinF I (10,000 U/ml) in NEBuffer 4 at 37°C for 16 h, or with 1 µl Purification:Article Title: A novel Gateway®-compatible binary vector allows direct selection of recombinant clones in Agrobacterium tumefaciens Article Snippet: The iProof ™ high fidelity Taq DNA polymerase (Bio-Rad, Hercules, CA) PCR program consisted of 1 cycle at 98°C (2 min), followed by 30 cycles at 98°C (30 s), 55°C (45 s), and 72°C (1 min), and finished with a 1 cycle extension at 72°C (7 min). .. The PCR products were digested with Bbv CI and Gel Purification:Article Title: A novel Gateway®-compatible binary vector allows direct selection of recombinant clones in Agrobacterium tumefaciens Article Snippet: The iProof ™ high fidelity Taq DNA polymerase (Bio-Rad, Hercules, CA) PCR program consisted of 1 cycle at 98°C (2 min), followed by 30 cycles at 98°C (30 s), 55°C (45 s), and 72°C (1 min), and finished with a 1 cycle extension at 72°C (7 min). .. The PCR products were digested with Bbv CI and Oligonucleotide Synthesis:Article Title: Cross-Link Structure Affects Replication-Independent DNA Interstrand Cross-Link Repair in Mammalian Cells † Article Snippet: We found that ICLs that covalently block the hydrogen bond face of the base display significantly lower repair efficiencies than ICLs that do not interfere with the hydrogen bond face. .. Protected deoxyribonucleoside 3′-O-phosphoramidites and oligonucleotide synthesis reagents were obtained from Glen Research, Inc. Polynucleotide kinase, T4 DNA ligase, E. coli DNA polymerase I (Klenow fragment), EcoRI, and Clone Assay:Article Title: Dynamic protein quantitation (DyProQ) of procollagen-I by CRISPR-Cas9 NanoLuciferase tagging Article Snippet: Generation of Split GFP Expressing Stable Cells To allow detection of CRISPR edited cells we included a split GFP tag developed in the Bo Huang lab . .. The sfGFP1-10 barrel was synthesised and cloned into a lentiviral vector (Vectorbuilder), and further subcloned into a CMV driven vector (pLenti CMV V5-LUC Blast (w567-1) was a gift from Eric Campeau (Addgene plasmid #21474 ; http://n2t.net/addgene:21474 ; RRID:Addgene_21474 )), briefly FLuc was removed from the vector by digesting with Plasmid Preparation:Article Title: Dynamic protein quantitation (DyProQ) of procollagen-I by CRISPR-Cas9 NanoLuciferase tagging Article Snippet: Generation of Split GFP Expressing Stable Cells To allow detection of CRISPR edited cells we included a split GFP tag developed in the Bo Huang lab . .. The sfGFP1-10 barrel was synthesised and cloned into a lentiviral vector (Vectorbuilder), and further subcloned into a CMV driven vector (pLenti CMV V5-LUC Blast (w567-1) was a gift from Eric Campeau (Addgene plasmid #21474 ; http://n2t.net/addgene:21474 ; RRID:Addgene_21474 )), briefly FLuc was removed from the vector by digesting with Amplification:Article Title: Dynamic protein quantitation (DyProQ) of procollagen-I by CRISPR-Cas9 NanoLuciferase tagging Article Snippet: Generation of Split GFP Expressing Stable Cells To allow detection of CRISPR edited cells we included a split GFP tag developed in the Bo Huang lab . .. The sfGFP1-10 barrel was synthesised and cloned into a lentiviral vector (Vectorbuilder), and further subcloned into a CMV driven vector (pLenti CMV V5-LUC Blast (w567-1) was a gift from Eric Campeau (Addgene plasmid #21474 ; http://n2t.net/addgene:21474 ; RRID:Addgene_21474 )), briefly FLuc was removed from the vector by digesting with Expressing:Article Title: Dynamic protein quantitation (DyProQ) of procollagen-I by CRISPR-Cas9 NanoLuciferase tagging Article Snippet: Generation of Split GFP Expressing Stable Cells To allow detection of CRISPR edited cells we included a split GFP tag developed in the Bo Huang lab . .. The sfGFP1-10 barrel was synthesised and cloned into a lentiviral vector (Vectorbuilder), and further subcloned into a CMV driven vector (pLenti CMV V5-LUC Blast (w567-1) was a gift from Eric Campeau (Addgene plasmid #21474 ; http://n2t.net/addgene:21474 ; RRID:Addgene_21474 )), briefly FLuc was removed from the vector by digesting with Incubation:Article Title: Methanococcus jannaschii Flap Endonuclease: Expression, Purification, and Substrate Requirements Article Snippet: φX174 virion DNA was annealed to oligonucleotide P-6 containing a branch point adjacent to a Bst XI recognition sequence and extended by 3′-5′ exonuclease-negative Pfu DNA polymerase (Stratagene) for 18 cycles of 55°C for 1 min and 65°C for 25 min. An aliquot was digested by Hae III and analyzed by agarose gel electrophoresis to verify conversion to duplex DNA. .. Various concentrations of FEN and 500 ng of branched duplex φX174 were incubated in 50 mM KCl–10 mM Tris-HCl (pH 8.0)–2.5 mM MgCl2 –1 mM DTT–10 μg of bovine serum albumin (BSA) per ml at 55°C for 1 h and digested with 1 U each of Article Title: A Potential Role for CHH DNA Methylation in Cotton Fiber Growth Patterns Article Snippet: Methylation-sensitive Endonuclease Digestion Genomic DNA was isolated from 7-dpa cotton ovules harvested at different times of the year. .. Aliquots (1 µg) were digested by incubation with 1 µl Bsl I (10,000 U/ml) in NEBuffer 3 at 55°C for 16 h, with 1 µl HinF I (10,000 U/ml) in NEBuffer 4 at 37°C for 16 h, or with 1 µl Agarose Gel Electrophoresis:Article Title: Methanococcus jannaschii Flap Endonuclease: Expression, Purification, and Substrate Requirements Article Snippet: φX174 virion DNA was annealed to oligonucleotide P-6 containing a branch point adjacent to a Bst XI recognition sequence and extended by 3′-5′ exonuclease-negative Pfu DNA polymerase (Stratagene) for 18 cycles of 55°C for 1 min and 65°C for 25 min. An aliquot was digested by Hae III and analyzed by agarose gel electrophoresis to verify conversion to duplex DNA. .. Various concentrations of FEN and 500 ng of branched duplex φX174 were incubated in 50 mM KCl–10 mM Tris-HCl (pH 8.0)–2.5 mM MgCl2 –1 mM DTT–10 μg of bovine serum albumin (BSA) per ml at 55°C for 1 h and digested with 1 U each of Staining:Article Title: Methanococcus jannaschii Flap Endonuclease: Expression, Purification, and Substrate Requirements Article Snippet: φX174 virion DNA was annealed to oligonucleotide P-6 containing a branch point adjacent to a Bst XI recognition sequence and extended by 3′-5′ exonuclease-negative Pfu DNA polymerase (Stratagene) for 18 cycles of 55°C for 1 min and 65°C for 25 min. An aliquot was digested by Hae III and analyzed by agarose gel electrophoresis to verify conversion to duplex DNA. .. Various concentrations of FEN and 500 ng of branched duplex φX174 were incubated in 50 mM KCl–10 mM Tris-HCl (pH 8.0)–2.5 mM MgCl2 –1 mM DTT–10 μg of bovine serum albumin (BSA) per ml at 55°C for 1 h and digested with 1 U each of Recombinant:Article Title: Sirtuin 1 Is a Key Regulator of the Interleukin-12 p70/Interleukin-23 Balance in Human Dendritic Cells * Article Snippet: 3-Aminopyridine adenine dinucleotide (AAD), a NAD+ analog that increases the cellular concentration of NAD+ by inhibiting dehydrogenases , was synthesized from nicotinic acid adenine dinucleotide and sodium azide as described ( ). .. Recombinant histone H3.3, Concentration Assay:Article Title: Strategy for Systematic Assembly of Large RNA and DNA Genomes: Transmissible Gastroenteritis Virus Model Article Snippet: A consensus sequence for each of the cloned fragments was determined, and when necessary (i.e., pTGEV A, pTGEV B1, pTGEV C, and pTGEV F), a consensus clone was assembled using restriction enzymes and standard recombinant DNA techniques to remove unwanted amino acid changes associated with reverse transcription or naturally occurring quasispecies variation. .. Each of the plasmids was grown to high concentration, isolated, and digested or double-digested with Bgl I, Isolation:Article Title: Strategy for Systematic Assembly of Large RNA and DNA Genomes: Transmissible Gastroenteritis Virus Model Article Snippet: A consensus sequence for each of the cloned fragments was determined, and when necessary (i.e., pTGEV A, pTGEV B1, pTGEV C, and pTGEV F), a consensus clone was assembled using restriction enzymes and standard recombinant DNA techniques to remove unwanted amino acid changes associated with reverse transcription or naturally occurring quasispecies variation. .. Each of the plasmids was grown to high concentration, isolated, and digested or double-digested with Bgl I, |
![Marker mutations are present in virus derived from the infectious construct. Cultures of ST cells were infected with wild-type (WT) TGEV or plaque-purified icTGEV isolates derived from the infectious construct. Intracellular RNA was isolated, and RT-PCR was performed using primer pairs that asymmetrically flank each of the unique Bgl I- Bst XI junctions inserted into the infectious construct. (A) Wild-type TGEV. (B) icTGEV-1 (passage 1). (C) icTGEV-3 (passage 3). In panel C, a larger ∼1.6-kb wild-type TGEV amplicon spanning the B1-B2 junction was also treated with Bst ]). Arrows indicate cleaved DNA intermediates.](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC110934/bin/jv2200918008.jpg)
