scrfi  (New England Biolabs)


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    Name:
    ScrFI
    Description:
    ScrFI 1 000 units
    Catalog Number:
    R0110S
    Price:
    69
    Category:
    Restriction Enzymes
    Size:
    1 000 units
    Buy from Supplier


    Structured Review

    New England Biolabs scrfi
    ScrFI
    ScrFI 1 000 units
    https://www.bioz.com/result/scrfi/product/New England Biolabs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    scrfi - by Bioz Stars, 2021-05
    92/100 stars

    Images

    1) Product Images from "An association study between CHEK2 gene mutations and susceptibility to breast cancer"

    Article Title: An association study between CHEK2 gene mutations and susceptibility to breast cancer

    Journal: Comparative Clinical Pathology

    doi: 10.1007/s00580-017-2455-x

    a A normal DNA, B and H PCR product uncut with Pst1, C and K heterozygous mutant-type cut with Scrf1: 194 bp and 174 bp fragmented and E negative control (water) M DNA marker. b Heterozygous mutant-type: 194 bp and 174 bp fragment by screening of PCR products using restriction enzymes ScrfI and PstI; A1 , heterozygous patient; B1 , C , and D homozygous normal
    Figure Legend Snippet: a A normal DNA, B and H PCR product uncut with Pst1, C and K heterozygous mutant-type cut with Scrf1: 194 bp and 174 bp fragmented and E negative control (water) M DNA marker. b Heterozygous mutant-type: 194 bp and 174 bp fragment by screening of PCR products using restriction enzymes ScrfI and PstI; A1 , heterozygous patient; B1 , C , and D homozygous normal

    Techniques Used: Polymerase Chain Reaction, Mutagenesis, Negative Control, Marker

    Related Articles

    Polymerase Chain Reaction:

    Article Title: Developmental Xist induction is mediated by enhanced splicing
    Article Snippet: .. PCR products were digested with Ava1 (NEB, cat. no. R0152S) or ScrF1 (NEB, cat. no. R0110S) to cut Ogt or G6pdx respectively. .. Samples were purified using Agencourt AMPure XP magnetic beads (Beckman Coulter cat. no. A63881).

    Article Title: An association study between CHEK2 gene mutations and susceptibility to breast cancer
    Article Snippet: .. PCR products were separately incubated for 16 h with either ScrFI or PstI (New England Bio labs, Beverly, MA). ..

    Article Title: Associations between Interleukin-31 Gene Polymorphisms and Dilated Cardiomyopathy in a Chinese Population
    Article Snippet: The PCR condition was designed as 95°C for 4 min firstly, then 33 cycles at 95°C for 30 s, 60°C for 30 s, and 72°C for 30 s, and finally, 72°C for 10 min. .. Furthermore, the PCR products were digested in 37°C stable incubation by distinguished restriction enzyme MboII (New England Biolabs, Peking, China) for 30 minutes of rs4758680 and ScrFI (New England Biolabs, Peking, China) for 2 hours of rs7977932 as shown in , separately. .. Ultimately, the results were visually analyzed by 6% polyacrylamide gels in silver staining.

    Incubation:

    Article Title: An association study between CHEK2 gene mutations and susceptibility to breast cancer
    Article Snippet: .. PCR products were separately incubated for 16 h with either ScrFI or PstI (New England Bio labs, Beverly, MA). ..

    Article Title: Associations between Interleukin-31 Gene Polymorphisms and Dilated Cardiomyopathy in a Chinese Population
    Article Snippet: The PCR condition was designed as 95°C for 4 min firstly, then 33 cycles at 95°C for 30 s, 60°C for 30 s, and 72°C for 30 s, and finally, 72°C for 10 min. .. Furthermore, the PCR products were digested in 37°C stable incubation by distinguished restriction enzyme MboII (New England Biolabs, Peking, China) for 30 minutes of rs4758680 and ScrFI (New England Biolabs, Peking, China) for 2 hours of rs7977932 as shown in , separately. .. Ultimately, the results were visually analyzed by 6% polyacrylamide gels in silver staining.

    Amplification:

    Article Title: Comparison of Three Different Hepatitis C Virus Genotyping Methods: 5′ NCR PCR‐ RFLP, Core Type‐Specific PCR, and NS5b Sequencing in a Tertiary Care Hospital in South India
    Article Snippet: Amplification of this region produced a 174 base pair product which was visualized by gel electrophoresis. .. RFLP was performed using four restriction enzymes Mva I (Roche Diagnostics GmbH), ScrF I, Hinf I, and BstU I (New England Biolabs, Beverly, MA) after obtaining the amplified product . .. Amplification of this region produced a 174 base pair product which was visualized by gel electrophoresis.

    Article Title: Novel compound heterozygous mutations in MYO7A in a Chinese family with Usher syndrome type 1
    Article Snippet: DNA sequencing was performed with the BigDye Terminator Cycle Sequencing Kit on an ABI 3130 Genetic Analyzer (Applied Biosystems). .. Restriction fragment length polymorphism analysis For the c.3742G > A mutation, a 454 bp DNA fragment was amplified (forward: 5′-CGG CTG CCC TCA AAA TCC ACA T-3′; reverse: 5′-TGG CAG GTA AAG GCA TTG AGA CA-3′) and cut into 172 bp and 282 bp with BstXI (New England BioLabs Inc., Beverly, MA) at 37 °C for 4 h. For the c.6051+1G > A mutation, a 99 bp DNA fragment was amplified (forward: 5′-CCA CGG TGC CAG GGA AGG ATC-3′; reverse: 5′-CAA CGC TAG CTG TGC ACG AAG G-3′) and cut into 41 bp and 48 bp with ScrFI (New England BioLabs) at 37 °C for 4 h. Both mutations eliminated the respective restriction sites. .. Digested PCR products were run on 1.5% agarose gels.

    Mutagenesis:

    Article Title: Novel compound heterozygous mutations in MYO7A in a Chinese family with Usher syndrome type 1
    Article Snippet: DNA sequencing was performed with the BigDye Terminator Cycle Sequencing Kit on an ABI 3130 Genetic Analyzer (Applied Biosystems). .. Restriction fragment length polymorphism analysis For the c.3742G > A mutation, a 454 bp DNA fragment was amplified (forward: 5′-CGG CTG CCC TCA AAA TCC ACA T-3′; reverse: 5′-TGG CAG GTA AAG GCA TTG AGA CA-3′) and cut into 172 bp and 282 bp with BstXI (New England BioLabs Inc., Beverly, MA) at 37 °C for 4 h. For the c.6051+1G > A mutation, a 99 bp DNA fragment was amplified (forward: 5′-CCA CGG TGC CAG GGA AGG ATC-3′; reverse: 5′-CAA CGC TAG CTG TGC ACG AAG G-3′) and cut into 41 bp and 48 bp with ScrFI (New England BioLabs) at 37 °C for 4 h. Both mutations eliminated the respective restriction sites. .. Digested PCR products were run on 1.5% agarose gels.

    CTG Assay:

    Article Title: Novel compound heterozygous mutations in MYO7A in a Chinese family with Usher syndrome type 1
    Article Snippet: DNA sequencing was performed with the BigDye Terminator Cycle Sequencing Kit on an ABI 3130 Genetic Analyzer (Applied Biosystems). .. Restriction fragment length polymorphism analysis For the c.3742G > A mutation, a 454 bp DNA fragment was amplified (forward: 5′-CGG CTG CCC TCA AAA TCC ACA T-3′; reverse: 5′-TGG CAG GTA AAG GCA TTG AGA CA-3′) and cut into 172 bp and 282 bp with BstXI (New England BioLabs Inc., Beverly, MA) at 37 °C for 4 h. For the c.6051+1G > A mutation, a 99 bp DNA fragment was amplified (forward: 5′-CCA CGG TGC CAG GGA AGG ATC-3′; reverse: 5′-CAA CGC TAG CTG TGC ACG AAG G-3′) and cut into 41 bp and 48 bp with ScrFI (New England BioLabs) at 37 °C for 4 h. Both mutations eliminated the respective restriction sites. .. Digested PCR products were run on 1.5% agarose gels.

    Countercurrent Chromatography:

    Article Title: Novel compound heterozygous mutations in MYO7A in a Chinese family with Usher syndrome type 1
    Article Snippet: DNA sequencing was performed with the BigDye Terminator Cycle Sequencing Kit on an ABI 3130 Genetic Analyzer (Applied Biosystems). .. Restriction fragment length polymorphism analysis For the c.3742G > A mutation, a 454 bp DNA fragment was amplified (forward: 5′-CGG CTG CCC TCA AAA TCC ACA T-3′; reverse: 5′-TGG CAG GTA AAG GCA TTG AGA CA-3′) and cut into 172 bp and 282 bp with BstXI (New England BioLabs Inc., Beverly, MA) at 37 °C for 4 h. For the c.6051+1G > A mutation, a 99 bp DNA fragment was amplified (forward: 5′-CCA CGG TGC CAG GGA AGG ATC-3′; reverse: 5′-CAA CGC TAG CTG TGC ACG AAG G-3′) and cut into 41 bp and 48 bp with ScrFI (New England BioLabs) at 37 °C for 4 h. Both mutations eliminated the respective restriction sites. .. Digested PCR products were run on 1.5% agarose gels.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Combined infectious bronchitis virus Arkansas and Massachusetts serotype vaccination suppresses replication of Arkansas vaccine virus.
    Article Snippet: RT-PCR was performed to amplify a portion of the N gene as previously described (Toro et al., 2006) except the annealing temperature was held constant at 60°C and 40 cycles were performed. .. RT-PCR products were cleaved separately with restriction enzymes ScrFI, which cleaves the RT-PCR product of the Ark vaccine, and BanI, which cleaves the RT-PCR product of the Mass vaccine (see Figure 1(a and b)), in reaction buffer provided by the supplier (New England Biolabs, Ipswich, MA, USA). .. Cleavage products were observed by GelGreenTM staining after agarose gel electrophoresis.

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    New England Biolabs scrfi
    a A normal DNA, B and H <t>PCR</t> product uncut with Pst1, C and K heterozygous mutant-type cut with Scrf1: 194 bp and 174 bp fragmented and E negative control (water) M DNA marker. b Heterozygous mutant-type: 194 bp and 174 bp fragment by screening of PCR products using restriction enzymes <t>ScrfI</t> and PstI; A1 , heterozygous patient; B1 , C , and D homozygous normal
    Scrfi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/scrfi/product/New England Biolabs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    scrfi - by Bioz Stars, 2021-05
    92/100 stars
      Buy from Supplier

    Image Search Results


    a A normal DNA, B and H PCR product uncut with Pst1, C and K heterozygous mutant-type cut with Scrf1: 194 bp and 174 bp fragmented and E negative control (water) M DNA marker. b Heterozygous mutant-type: 194 bp and 174 bp fragment by screening of PCR products using restriction enzymes ScrfI and PstI; A1 , heterozygous patient; B1 , C , and D homozygous normal

    Journal: Comparative Clinical Pathology

    Article Title: An association study between CHEK2 gene mutations and susceptibility to breast cancer

    doi: 10.1007/s00580-017-2455-x

    Figure Lengend Snippet: a A normal DNA, B and H PCR product uncut with Pst1, C and K heterozygous mutant-type cut with Scrf1: 194 bp and 174 bp fragmented and E negative control (water) M DNA marker. b Heterozygous mutant-type: 194 bp and 174 bp fragment by screening of PCR products using restriction enzymes ScrfI and PstI; A1 , heterozygous patient; B1 , C , and D homozygous normal

    Article Snippet: PCR products were separately incubated for 16 h with either ScrFI or PstI (New England Bio labs, Beverly, MA).

    Techniques: Polymerase Chain Reaction, Mutagenesis, Negative Control, Marker