quick rna micro prep kit  (Zymo Research)


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    Name:
    Quick RNA Microprep Kit
    Description:
    The Quick RNA Microprep Kit is an innovative product designed for the easy reliable and rapid isolation of DNA free RNA from a wide range of cell up to 106 and tissue samples up to 5 mg The procedure combines a unique buffer system with Zymo Spin column technology to yield high quality total RNA including small RNAs 17 200 nt in about 10 minutes The procedure is simple Add the provided RNA Lysis Buffer to a sample and then purify the RNA using the Zymo Spin Columns The result is highly concentrated DNA free RNA that is suitable for RT PCR hybridization sequencing etc In addition the kit can be used for the enrichment of small and large RNAs into separate fractions
    Catalog Number:
    r1051
    Price:
    None
    Applications:
    RNA Purification
    Size:
    50 units
    Category:
    Life Science Reagents and Media
    Buy from Supplier


    Structured Review

    Zymo Research quick rna micro prep kit
    Quick RNA Microprep Kit
    The Quick RNA Microprep Kit is an innovative product designed for the easy reliable and rapid isolation of DNA free RNA from a wide range of cell up to 106 and tissue samples up to 5 mg The procedure combines a unique buffer system with Zymo Spin column technology to yield high quality total RNA including small RNAs 17 200 nt in about 10 minutes The procedure is simple Add the provided RNA Lysis Buffer to a sample and then purify the RNA using the Zymo Spin Columns The result is highly concentrated DNA free RNA that is suitable for RT PCR hybridization sequencing etc In addition the kit can be used for the enrichment of small and large RNAs into separate fractions
    https://www.bioz.com/result/quick rna micro prep kit/product/Zymo Research
    Average 99 stars, based on 144 article reviews
    Price from $9.99 to $1999.99
    quick rna micro prep kit - by Bioz Stars, 2020-10
    99/100 stars

    Images

    1) Product Images from "Loss of mitochondrial protease CLPP activates type I interferon responses through the mtDNA-cGAS-STING signaling axis"

    Article Title: Loss of mitochondrial protease CLPP activates type I interferon responses through the mtDNA-cGAS-STING signaling axis

    Journal: bioRxiv

    doi: 10.1101/2020.08.30.274712

    CLPP-KO fibroblasts are more resistant to viral infection owing to elevated activation of the STING pathway. A Quantification of GFP staining in MEFS at MOI 0.1 24hrs after VSV infection. B Quantitative real-time PCR of viral RNA at MOI 0.1 16hrs after VSV infection. C Quantitative real-time PCR of ISGs 24hrs after VSV infection at MOI of 0.1. D Quantitative real-time PCR of viral RNA in MEFs infected with HSV-1 at 48hpi and MOI of 0.01. Data information: In (B, C) Real-time PCR data are presented as relative expression percentage (%) comparing CLPP-KO vs WT and CLPP-KO/STING gt/gt vs STING gt/gt MEFs. Error bars indicate ± s.e.m. of triplicate technical replicates (Student’s t-test). In (A, C) data are presented as mean ± s.e.m. of triplicate technical replicates (One-way ANOVA Tukey–8217;s post hoc ). *p
    Figure Legend Snippet: CLPP-KO fibroblasts are more resistant to viral infection owing to elevated activation of the STING pathway. A Quantification of GFP staining in MEFS at MOI 0.1 24hrs after VSV infection. B Quantitative real-time PCR of viral RNA at MOI 0.1 16hrs after VSV infection. C Quantitative real-time PCR of ISGs 24hrs after VSV infection at MOI of 0.1. D Quantitative real-time PCR of viral RNA in MEFs infected with HSV-1 at 48hpi and MOI of 0.01. Data information: In (B, C) Real-time PCR data are presented as relative expression percentage (%) comparing CLPP-KO vs WT and CLPP-KO/STING gt/gt vs STING gt/gt MEFs. Error bars indicate ± s.e.m. of triplicate technical replicates (Student’s t-test). In (A, C) data are presented as mean ± s.e.m. of triplicate technical replicates (One-way ANOVA Tukey–8217;s post hoc ). *p

    Techniques Used: Infection, Activation Assay, Staining, Real-time Polymerase Chain Reaction, Expressing

    CLPP-KO fibroblasts are more resistant to viral infection owing to elevated activation of the STING pathway. A-C Analysis of viral load and viral plaques in MEFs after 24hrs of VSV infection at multiplicity of infection (MOI) of 0.1. (A) Microscopy images of viral GFP expression in MEFs infected with VSV. DAPI was used for nuclear staining. (B) Quantitative real-time PCR of viral RNA transcripts, VSV-G and VSV-M , after VSV infection (n=3 MEF lines). (C) Quantification of plaque assays from VSV infected MEFs. D Quantitative real-time PCR of viral RNA in MEFs infected with HSV-1 at 72hrs post infection (hpi) and MOI of 0.001 (n=3 MEF lines). E Quantification of plaque assay from HSV-1 infected MEFs. Plaque figure is representative of 10 −2 supernatant dilution and graphed as pfu/ml (n=2). Data information: In (C, E), plaque figure is representative of 10 −5 supernatant dilution and graphed as pfu/ml. In (B, D), Real-time PCR data was represented as relative expression percentage (%) comparing CLPP-KO vs WT and CLPP-KO/STING gt/gt vs STING gt/gt MEFs. In (B-E), data are presented as mean ± s.e.m. of triplicate technical replicates. **p
    Figure Legend Snippet: CLPP-KO fibroblasts are more resistant to viral infection owing to elevated activation of the STING pathway. A-C Analysis of viral load and viral plaques in MEFs after 24hrs of VSV infection at multiplicity of infection (MOI) of 0.1. (A) Microscopy images of viral GFP expression in MEFs infected with VSV. DAPI was used for nuclear staining. (B) Quantitative real-time PCR of viral RNA transcripts, VSV-G and VSV-M , after VSV infection (n=3 MEF lines). (C) Quantification of plaque assays from VSV infected MEFs. D Quantitative real-time PCR of viral RNA in MEFs infected with HSV-1 at 72hrs post infection (hpi) and MOI of 0.001 (n=3 MEF lines). E Quantification of plaque assay from HSV-1 infected MEFs. Plaque figure is representative of 10 −2 supernatant dilution and graphed as pfu/ml (n=2). Data information: In (C, E), plaque figure is representative of 10 −5 supernatant dilution and graphed as pfu/ml. In (B, D), Real-time PCR data was represented as relative expression percentage (%) comparing CLPP-KO vs WT and CLPP-KO/STING gt/gt vs STING gt/gt MEFs. In (B-E), data are presented as mean ± s.e.m. of triplicate technical replicates. **p

    Techniques Used: Infection, Activation Assay, Microscopy, Expressing, Staining, Real-time Polymerase Chain Reaction, Plaque Assay

    2) Product Images from "TET enzymes control antibody production and shape the mutational landscape in germinal centre B cells"

    Article Title: TET enzymes control antibody production and shape the mutational landscape in germinal centre B cells

    Journal: The Febs Journal

    doi: 10.1111/febs.14934

    mRNA expression of TET 2 and TET 3 in B cells ex vivo . B‐cell populations were FACS ‐sorted from bone marrow and spleens of 8‐wk‐young wild type mice (Development and Mature; n = 3) or spleens from 9‐wk‐young wild type mice that has been immunized with sheep RBC 7 days before (Antigen activated; n = 3), and RNA was isolated for qRT ‐ PCR analysis for (A) TET 2 and (B) TET 3 expression. HPRT was used as reference gene. Bone marrow: pro B cells, large and small pre B cells and immature IgM + B cells. Spleen: transitional 1 (T1) B cells, transitional 2 (T2) B cells, mature follicular ( FO ) B cells, marginal zone ( MZ ) B cells, germinal centre ( GC ) B cells and plasmablasts/plasma cells ( PC ). GC B cells were further divided into dark zone centroblasts ( CB ) and light zone centrocytes ( CC ). Data are shown as mean ± SD.
    Figure Legend Snippet: mRNA expression of TET 2 and TET 3 in B cells ex vivo . B‐cell populations were FACS ‐sorted from bone marrow and spleens of 8‐wk‐young wild type mice (Development and Mature; n = 3) or spleens from 9‐wk‐young wild type mice that has been immunized with sheep RBC 7 days before (Antigen activated; n = 3), and RNA was isolated for qRT ‐ PCR analysis for (A) TET 2 and (B) TET 3 expression. HPRT was used as reference gene. Bone marrow: pro B cells, large and small pre B cells and immature IgM + B cells. Spleen: transitional 1 (T1) B cells, transitional 2 (T2) B cells, mature follicular ( FO ) B cells, marginal zone ( MZ ) B cells, germinal centre ( GC ) B cells and plasmablasts/plasma cells ( PC ). GC B cells were further divided into dark zone centroblasts ( CB ) and light zone centrocytes ( CC ). Data are shown as mean ± SD.

    Techniques Used: Expressing, Ex Vivo, FACS, Mouse Assay, Isolation, Quantitative RT-PCR

    3) Product Images from "The Cat Flea ( Ctenocephalides felis) Immune Deficiency Signaling Pathway Regulates Rickettsia typhi Infection"

    Article Title: The Cat Flea ( Ctenocephalides felis) Immune Deficiency Signaling Pathway Regulates Rickettsia typhi Infection

    Journal: Infection and Immunity

    doi: 10.1128/IAI.00562-17

    R. typhi elicits an immune response in Drosophila cells. Shown are results of a PCR array analysis of transcripts in uninfected and R. typhi -infected KC167 cells. RNA was extracted from KC167 cells infected with R. typhi for 0 or 4 h, and cDNA was used in the Fruit Fly Signal Transduction PathwayFinder PCR array.
    Figure Legend Snippet: R. typhi elicits an immune response in Drosophila cells. Shown are results of a PCR array analysis of transcripts in uninfected and R. typhi -infected KC167 cells. RNA was extracted from KC167 cells infected with R. typhi for 0 or 4 h, and cDNA was used in the Fruit Fly Signal Transduction PathwayFinder PCR array.

    Techniques Used: Polymerase Chain Reaction, Infection, Transduction

    4) Product Images from "p53 Regulates the Expression of LRP1 and Apoptosis through a Stress Intensity-Dependent MicroRNA Feedback Loop"

    Article Title: p53 Regulates the Expression of LRP1 and Apoptosis through a Stress Intensity-Dependent MicroRNA Feedback Loop

    Journal: Cell reports

    doi: 10.1016/j.celrep.2018.07.010

    Lethal Dox Suppresses LRP1 Protein Translation through the p53-Regulated miRNAs MiR-103 and MiR-107 (A) HCT116 cells were treated with the indicated dose of Dox (nanomolar) for 24 hr, after which RNA was collected and subjected to qRT-PCR analysis. Data represent at least two biological replicates, each performed in triplicate. (B) HCT116 cells were treated with the indicated dose of Dox for 24 hr, after which cells were subjected to ChIP analysis using anti-p53 antibody and non-specific IgG. Data represent at least two biological replicates, each performed in triplicate. (C) HCT116 cells were treated with the indicated stress for 24 hr, after which vehicle, MG132 (MG), or CQ was added to the cells for an additional 8 hr. After MG or CQ treatment, lysates were collected and subjected to western blot analysis. Data represent at least two biological replicates. (D) HCT116 cells were treated with the indicated course of Dox for 24 hr, after which cells were labeled with 35 S-Met/Cys for 30 min, chased with complete DMEM for another 30 min, and then subjected to LRP1 immunoprecipitation. Data represent at least two biological replicates.
    Figure Legend Snippet: Lethal Dox Suppresses LRP1 Protein Translation through the p53-Regulated miRNAs MiR-103 and MiR-107 (A) HCT116 cells were treated with the indicated dose of Dox (nanomolar) for 24 hr, after which RNA was collected and subjected to qRT-PCR analysis. Data represent at least two biological replicates, each performed in triplicate. (B) HCT116 cells were treated with the indicated dose of Dox for 24 hr, after which cells were subjected to ChIP analysis using anti-p53 antibody and non-specific IgG. Data represent at least two biological replicates, each performed in triplicate. (C) HCT116 cells were treated with the indicated stress for 24 hr, after which vehicle, MG132 (MG), or CQ was added to the cells for an additional 8 hr. After MG or CQ treatment, lysates were collected and subjected to western blot analysis. Data represent at least two biological replicates. (D) HCT116 cells were treated with the indicated course of Dox for 24 hr, after which cells were labeled with 35 S-Met/Cys for 30 min, chased with complete DMEM for another 30 min, and then subjected to LRP1 immunoprecipitation. Data represent at least two biological replicates.

    Techniques Used: Quantitative RT-PCR, Chromatin Immunoprecipitation, Western Blot, Labeling, Immunoprecipitation

    5) Product Images from "FXR activation by obeticholic acid elevates liver LDL receptor expression by mRNA stabilization and reduces plasma LDL-cholesterol in mice"

    Article Title: FXR activation by obeticholic acid elevates liver LDL receptor expression by mRNA stabilization and reduces plasma LDL-cholesterol in mice

    Journal: Arteriosclerosis, thrombosis, and vascular biology

    doi: 10.1161/ATVBAHA.118.311122

    SREBP2-independent induction of LDLR mRNA expression by FXR agonists in human primary hepatocytes derived from five different donors. (A-C) HPHs of three different donors (HPH 4055B, HPH 4105A, HPH 4034) were treated with OCA at 1 and 10 μM for 24 h before isolation of total RNA. Triplicate wells were used in each condition. qRT-PCR was performed to measure relative mRNA levels of indicated genes with duplicate measurement of each cDNA sample. (D-E) HPH 4122B were treated with FXR agonists at indicated concentrations for 24 h before isolation of total RNA or total lysates. Duplicate wells were used in each condition. LDLR protein abundance was analyzed by Western blotting. Values represent mean of densitometric measurements of LDLR normalized to β-actin signal from duplicate samples per treatment. In D, Q-PCR was performed to measure relative LDLR mRNA levels. Individual cDNA samples were measured in duplicate and a total of 4 q-PCR measurements for each condition. (F) HPH 4269 were treated with OCA (10 μM) for 24 h before isolation of total RNA. Triplicate wells were used in each condition. Q-PCR was performed to measure relative mRNA levels of indicated genes with duplicate measurement of each cDNA sample. In A-D, statistical significance was determined with One-way Anova with Dunnett’s Multiple Comparison posttest. In F, statistical significance was determined by 2-tailed unpaired Student t test. N=3 in each group. * p value
    Figure Legend Snippet: SREBP2-independent induction of LDLR mRNA expression by FXR agonists in human primary hepatocytes derived from five different donors. (A-C) HPHs of three different donors (HPH 4055B, HPH 4105A, HPH 4034) were treated with OCA at 1 and 10 μM for 24 h before isolation of total RNA. Triplicate wells were used in each condition. qRT-PCR was performed to measure relative mRNA levels of indicated genes with duplicate measurement of each cDNA sample. (D-E) HPH 4122B were treated with FXR agonists at indicated concentrations for 24 h before isolation of total RNA or total lysates. Duplicate wells were used in each condition. LDLR protein abundance was analyzed by Western blotting. Values represent mean of densitometric measurements of LDLR normalized to β-actin signal from duplicate samples per treatment. In D, Q-PCR was performed to measure relative LDLR mRNA levels. Individual cDNA samples were measured in duplicate and a total of 4 q-PCR measurements for each condition. (F) HPH 4269 were treated with OCA (10 μM) for 24 h before isolation of total RNA. Triplicate wells were used in each condition. Q-PCR was performed to measure relative mRNA levels of indicated genes with duplicate measurement of each cDNA sample. In A-D, statistical significance was determined with One-way Anova with Dunnett’s Multiple Comparison posttest. In F, statistical significance was determined by 2-tailed unpaired Student t test. N=3 in each group. * p value

    Techniques Used: Expressing, Derivative Assay, Isolation, Quantitative RT-PCR, Western Blot, Polymerase Chain Reaction

    Related Articles

    RNA Extraction:

    Article Title: Kat2a and Kat2b Acetyltransferase Activity Regulates Craniofacial Cartilage and Bone Differentiation in Zebrafish and Mice
    Article Snippet: .. Tissue dissected from embryos was flash frozen in liquid nitrogen, followed by RNA extraction with the Zymo Quick RNA kit (Zymo, Cat #R1050, Irvine, CA USA). cDNA was prepared from 1 μg of RNA with the Superscript III Reverse Transcriptase kit (Thermo Fisher, Cat #18080093). qPCR was carried out using Roche Universal Probe library Master Mix and Roche Light Cycler 480. ..

    Article Title: BLNCR is a long non-coding RNA adjacent to integrin beta-1 that is rapidly lost during epidermal progenitor cell differentiation
    Article Snippet: .. RNA extraction was performed using the Quick RNA Microprep kit (Zymo Research) according to manufacturer’s protocol. .. Reverse transcription was performed using Maxima RT enzyme (Thermo Fisher Scientific) according to manufacturer’s protocol followed by qPCR.

    RNA Sequencing Assay:

    Article Title: Age-related islet inflammation marks the proliferative decline of pancreatic beta-cells in zebrafish
    Article Snippet: .. For RNA-Sequencing, total RNA was extracted from FACS sorted beta-cells using Quick-RNA MicroPrep kit (R1050 Zymo Research). .. Sequencing was performed on llumina HiSeq2500 in 2 × 75 bp paired-end mode.

    Isolation:

    Article Title: Loss of mitochondrial protease CLPP activates type I interferon responses through the mtDNA-cGAS-STING signaling axis
    Article Snippet: .. Quantitative PCR To measure relative gene expression by qRT-PCR, total cellular RNA was isolated using the Quick-RNA Micro Prep Kit (R1051, Zymo Research). .. Approximately 250-500ng RNA was normalized across samples and cDNA was generated using the qScript cDNA Synthesis Kit (101414-098, VWR). cDNA was then subjected to qPCR using PerfecTa SYBR Green FastMix (84069, Quantabio) and primers as indicated in .

    Article Title: A direct regulatory link between microRNA-137 and SHANK2: implications for neuropsychiatric disorders
    Article Snippet: .. For the quantification of Shank2 expression, RNA was isolated from mouse primary hippocampal neurons (DIV8) 72 h after transfection with using the Quick-RNA™ MicroPrep Kit (Zymo Research, Freiburg, Germany) and was transcribed with the SuperScript® VILO ™ cDNA Synthesis Kit (Thermo Fisher Scientific, Darmstadt, Germany). .. RT-qPCR was performed to quantify Shank2 expression normalized against two reference genes ( Gapdh and Hprt1 ).

    Article Title: TET enzymes control antibody production and shape the mutational landscape in germinal centre B cells
    Article Snippet: .. Quantitative real‐time‐PCR RNA from snap‐frozen cell pellets of FACS‐sorted B cells was isolated using the Quick‐RNA Micro Prep Kit (Zymo Research, Irvine, CA, USA, R1050) and DNase digestion as per manufacturer's instructions. .. RNA from snap‐frozen in vitro ‐cultivated B cells was isolated using Trizol reagent (Thermo Fisher Scientific, 15596026) as per manufacturer's instructions.

    Transfection:

    Article Title: A direct regulatory link between microRNA-137 and SHANK2: implications for neuropsychiatric disorders
    Article Snippet: .. For the quantification of Shank2 expression, RNA was isolated from mouse primary hippocampal neurons (DIV8) 72 h after transfection with using the Quick-RNA™ MicroPrep Kit (Zymo Research, Freiburg, Germany) and was transcribed with the SuperScript® VILO ™ cDNA Synthesis Kit (Thermo Fisher Scientific, Darmstadt, Germany). .. RT-qPCR was performed to quantify Shank2 expression normalized against two reference genes ( Gapdh and Hprt1 ).

    Quantitative RT-PCR:

    Article Title: Loss of mitochondrial protease CLPP activates type I interferon responses through the mtDNA-cGAS-STING signaling axis
    Article Snippet: .. Quantitative PCR To measure relative gene expression by qRT-PCR, total cellular RNA was isolated using the Quick-RNA Micro Prep Kit (R1051, Zymo Research). .. Approximately 250-500ng RNA was normalized across samples and cDNA was generated using the qScript cDNA Synthesis Kit (101414-098, VWR). cDNA was then subjected to qPCR using PerfecTa SYBR Green FastMix (84069, Quantabio) and primers as indicated in .

    Real-time Polymerase Chain Reaction:

    Article Title: Loss of mitochondrial protease CLPP activates type I interferon responses through the mtDNA-cGAS-STING signaling axis
    Article Snippet: .. Quantitative PCR To measure relative gene expression by qRT-PCR, total cellular RNA was isolated using the Quick-RNA Micro Prep Kit (R1051, Zymo Research). .. Approximately 250-500ng RNA was normalized across samples and cDNA was generated using the qScript cDNA Synthesis Kit (101414-098, VWR). cDNA was then subjected to qPCR using PerfecTa SYBR Green FastMix (84069, Quantabio) and primers as indicated in .

    Article Title: Kat2a and Kat2b Acetyltransferase Activity Regulates Craniofacial Cartilage and Bone Differentiation in Zebrafish and Mice
    Article Snippet: .. Tissue dissected from embryos was flash frozen in liquid nitrogen, followed by RNA extraction with the Zymo Quick RNA kit (Zymo, Cat #R1050, Irvine, CA USA). cDNA was prepared from 1 μg of RNA with the Superscript III Reverse Transcriptase kit (Thermo Fisher, Cat #18080093). qPCR was carried out using Roche Universal Probe library Master Mix and Roche Light Cycler 480. ..

    Article Title: TET enzymes control antibody production and shape the mutational landscape in germinal centre B cells
    Article Snippet: .. Quantitative real‐time‐PCR RNA from snap‐frozen cell pellets of FACS‐sorted B cells was isolated using the Quick‐RNA Micro Prep Kit (Zymo Research, Irvine, CA, USA, R1050) and DNase digestion as per manufacturer's instructions. .. RNA from snap‐frozen in vitro ‐cultivated B cells was isolated using Trizol reagent (Thermo Fisher Scientific, 15596026) as per manufacturer's instructions.

    Expressing:

    Article Title: Loss of mitochondrial protease CLPP activates type I interferon responses through the mtDNA-cGAS-STING signaling axis
    Article Snippet: .. Quantitative PCR To measure relative gene expression by qRT-PCR, total cellular RNA was isolated using the Quick-RNA Micro Prep Kit (R1051, Zymo Research). .. Approximately 250-500ng RNA was normalized across samples and cDNA was generated using the qScript cDNA Synthesis Kit (101414-098, VWR). cDNA was then subjected to qPCR using PerfecTa SYBR Green FastMix (84069, Quantabio) and primers as indicated in .

    Article Title: A direct regulatory link between microRNA-137 and SHANK2: implications for neuropsychiatric disorders
    Article Snippet: .. For the quantification of Shank2 expression, RNA was isolated from mouse primary hippocampal neurons (DIV8) 72 h after transfection with using the Quick-RNA™ MicroPrep Kit (Zymo Research, Freiburg, Germany) and was transcribed with the SuperScript® VILO ™ cDNA Synthesis Kit (Thermo Fisher Scientific, Darmstadt, Germany). .. RT-qPCR was performed to quantify Shank2 expression normalized against two reference genes ( Gapdh and Hprt1 ).

    FACS:

    Article Title: Age-related islet inflammation marks the proliferative decline of pancreatic beta-cells in zebrafish
    Article Snippet: .. For RNA-Sequencing, total RNA was extracted from FACS sorted beta-cells using Quick-RNA MicroPrep kit (R1050 Zymo Research). .. Sequencing was performed on llumina HiSeq2500 in 2 × 75 bp paired-end mode.

    Article Title: TET enzymes control antibody production and shape the mutational landscape in germinal centre B cells
    Article Snippet: .. Quantitative real‐time‐PCR RNA from snap‐frozen cell pellets of FACS‐sorted B cells was isolated using the Quick‐RNA Micro Prep Kit (Zymo Research, Irvine, CA, USA, R1050) and DNase digestion as per manufacturer's instructions. .. RNA from snap‐frozen in vitro ‐cultivated B cells was isolated using Trizol reagent (Thermo Fisher Scientific, 15596026) as per manufacturer's instructions.

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    Zymo Research quick rna micro prep kit
    CLPP-KO fibroblasts are more resistant to viral infection owing to elevated activation of the STING pathway. A Quantification of GFP staining in MEFS at MOI 0.1 24hrs after VSV infection. B Quantitative real-time <t>PCR</t> of viral <t>RNA</t> at MOI 0.1 16hrs after VSV infection. C Quantitative real-time PCR of ISGs 24hrs after VSV infection at MOI of 0.1. D Quantitative real-time PCR of viral RNA in MEFs infected with HSV-1 at 48hpi and MOI of 0.01. Data information: In (B, C) Real-time PCR data are presented as relative expression percentage (%) comparing CLPP-KO vs WT and CLPP-KO/STING gt/gt vs STING gt/gt MEFs. Error bars indicate ± s.e.m. of triplicate technical replicates (Student’s t-test). In (A, C) data are presented as mean ± s.e.m. of triplicate technical replicates (One-way ANOVA Tukey–8217;s post hoc ). *p
    Quick Rna Micro Prep Kit, supplied by Zymo Research, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quick rna micro prep kit/product/Zymo Research
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    quick rna micro prep kit - by Bioz Stars, 2020-10
    99/100 stars
      Buy from Supplier

    99
    Zymo Research quick rna mini prep plus kit
    R. typhi elicits an immune response in Drosophila cells. Shown are results of a PCR array analysis of transcripts in uninfected and R. typhi -infected <t>KC167</t> cells. <t>RNA</t> was extracted from KC167 cells infected with R. typhi for 0 or 4 h, and cDNA was used in the Fruit Fly Signal Transduction PathwayFinder PCR array.
    Quick Rna Mini Prep Plus Kit, supplied by Zymo Research, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quick rna mini prep plus kit/product/Zymo Research
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    quick rna mini prep plus kit - by Bioz Stars, 2020-10
    99/100 stars
      Buy from Supplier

    Image Search Results


    CLPP-KO fibroblasts are more resistant to viral infection owing to elevated activation of the STING pathway. A Quantification of GFP staining in MEFS at MOI 0.1 24hrs after VSV infection. B Quantitative real-time PCR of viral RNA at MOI 0.1 16hrs after VSV infection. C Quantitative real-time PCR of ISGs 24hrs after VSV infection at MOI of 0.1. D Quantitative real-time PCR of viral RNA in MEFs infected with HSV-1 at 48hpi and MOI of 0.01. Data information: In (B, C) Real-time PCR data are presented as relative expression percentage (%) comparing CLPP-KO vs WT and CLPP-KO/STING gt/gt vs STING gt/gt MEFs. Error bars indicate ± s.e.m. of triplicate technical replicates (Student’s t-test). In (A, C) data are presented as mean ± s.e.m. of triplicate technical replicates (One-way ANOVA Tukey–8217;s post hoc ). *p

    Journal: bioRxiv

    Article Title: Loss of mitochondrial protease CLPP activates type I interferon responses through the mtDNA-cGAS-STING signaling axis

    doi: 10.1101/2020.08.30.274712

    Figure Lengend Snippet: CLPP-KO fibroblasts are more resistant to viral infection owing to elevated activation of the STING pathway. A Quantification of GFP staining in MEFS at MOI 0.1 24hrs after VSV infection. B Quantitative real-time PCR of viral RNA at MOI 0.1 16hrs after VSV infection. C Quantitative real-time PCR of ISGs 24hrs after VSV infection at MOI of 0.1. D Quantitative real-time PCR of viral RNA in MEFs infected with HSV-1 at 48hpi and MOI of 0.01. Data information: In (B, C) Real-time PCR data are presented as relative expression percentage (%) comparing CLPP-KO vs WT and CLPP-KO/STING gt/gt vs STING gt/gt MEFs. Error bars indicate ± s.e.m. of triplicate technical replicates (Student’s t-test). In (A, C) data are presented as mean ± s.e.m. of triplicate technical replicates (One-way ANOVA Tukey–8217;s post hoc ). *p

    Article Snippet: Quantitative PCR To measure relative gene expression by qRT-PCR, total cellular RNA was isolated using the Quick-RNA Micro Prep Kit (R1051, Zymo Research).

    Techniques: Infection, Activation Assay, Staining, Real-time Polymerase Chain Reaction, Expressing

    CLPP-KO fibroblasts are more resistant to viral infection owing to elevated activation of the STING pathway. A-C Analysis of viral load and viral plaques in MEFs after 24hrs of VSV infection at multiplicity of infection (MOI) of 0.1. (A) Microscopy images of viral GFP expression in MEFs infected with VSV. DAPI was used for nuclear staining. (B) Quantitative real-time PCR of viral RNA transcripts, VSV-G and VSV-M , after VSV infection (n=3 MEF lines). (C) Quantification of plaque assays from VSV infected MEFs. D Quantitative real-time PCR of viral RNA in MEFs infected with HSV-1 at 72hrs post infection (hpi) and MOI of 0.001 (n=3 MEF lines). E Quantification of plaque assay from HSV-1 infected MEFs. Plaque figure is representative of 10 −2 supernatant dilution and graphed as pfu/ml (n=2). Data information: In (C, E), plaque figure is representative of 10 −5 supernatant dilution and graphed as pfu/ml. In (B, D), Real-time PCR data was represented as relative expression percentage (%) comparing CLPP-KO vs WT and CLPP-KO/STING gt/gt vs STING gt/gt MEFs. In (B-E), data are presented as mean ± s.e.m. of triplicate technical replicates. **p

    Journal: bioRxiv

    Article Title: Loss of mitochondrial protease CLPP activates type I interferon responses through the mtDNA-cGAS-STING signaling axis

    doi: 10.1101/2020.08.30.274712

    Figure Lengend Snippet: CLPP-KO fibroblasts are more resistant to viral infection owing to elevated activation of the STING pathway. A-C Analysis of viral load and viral plaques in MEFs after 24hrs of VSV infection at multiplicity of infection (MOI) of 0.1. (A) Microscopy images of viral GFP expression in MEFs infected with VSV. DAPI was used for nuclear staining. (B) Quantitative real-time PCR of viral RNA transcripts, VSV-G and VSV-M , after VSV infection (n=3 MEF lines). (C) Quantification of plaque assays from VSV infected MEFs. D Quantitative real-time PCR of viral RNA in MEFs infected with HSV-1 at 72hrs post infection (hpi) and MOI of 0.001 (n=3 MEF lines). E Quantification of plaque assay from HSV-1 infected MEFs. Plaque figure is representative of 10 −2 supernatant dilution and graphed as pfu/ml (n=2). Data information: In (C, E), plaque figure is representative of 10 −5 supernatant dilution and graphed as pfu/ml. In (B, D), Real-time PCR data was represented as relative expression percentage (%) comparing CLPP-KO vs WT and CLPP-KO/STING gt/gt vs STING gt/gt MEFs. In (B-E), data are presented as mean ± s.e.m. of triplicate technical replicates. **p

    Article Snippet: Quantitative PCR To measure relative gene expression by qRT-PCR, total cellular RNA was isolated using the Quick-RNA Micro Prep Kit (R1051, Zymo Research).

    Techniques: Infection, Activation Assay, Microscopy, Expressing, Staining, Real-time Polymerase Chain Reaction, Plaque Assay

    mRNA expression of TET 2 and TET 3 in B cells ex vivo . B‐cell populations were FACS ‐sorted from bone marrow and spleens of 8‐wk‐young wild type mice (Development and Mature; n = 3) or spleens from 9‐wk‐young wild type mice that has been immunized with sheep RBC 7 days before (Antigen activated; n = 3), and RNA was isolated for qRT ‐ PCR analysis for (A) TET 2 and (B) TET 3 expression. HPRT was used as reference gene. Bone marrow: pro B cells, large and small pre B cells and immature IgM + B cells. Spleen: transitional 1 (T1) B cells, transitional 2 (T2) B cells, mature follicular ( FO ) B cells, marginal zone ( MZ ) B cells, germinal centre ( GC ) B cells and plasmablasts/plasma cells ( PC ). GC B cells were further divided into dark zone centroblasts ( CB ) and light zone centrocytes ( CC ). Data are shown as mean ± SD.

    Journal: The Febs Journal

    Article Title: TET enzymes control antibody production and shape the mutational landscape in germinal centre B cells

    doi: 10.1111/febs.14934

    Figure Lengend Snippet: mRNA expression of TET 2 and TET 3 in B cells ex vivo . B‐cell populations were FACS ‐sorted from bone marrow and spleens of 8‐wk‐young wild type mice (Development and Mature; n = 3) or spleens from 9‐wk‐young wild type mice that has been immunized with sheep RBC 7 days before (Antigen activated; n = 3), and RNA was isolated for qRT ‐ PCR analysis for (A) TET 2 and (B) TET 3 expression. HPRT was used as reference gene. Bone marrow: pro B cells, large and small pre B cells and immature IgM + B cells. Spleen: transitional 1 (T1) B cells, transitional 2 (T2) B cells, mature follicular ( FO ) B cells, marginal zone ( MZ ) B cells, germinal centre ( GC ) B cells and plasmablasts/plasma cells ( PC ). GC B cells were further divided into dark zone centroblasts ( CB ) and light zone centrocytes ( CC ). Data are shown as mean ± SD.

    Article Snippet: Quantitative real‐time‐PCR RNA from snap‐frozen cell pellets of FACS‐sorted B cells was isolated using the Quick‐RNA Micro Prep Kit (Zymo Research, Irvine, CA, USA, R1050) and DNase digestion as per manufacturer's instructions.

    Techniques: Expressing, Ex Vivo, FACS, Mouse Assay, Isolation, Quantitative RT-PCR

    R. typhi elicits an immune response in Drosophila cells. Shown are results of a PCR array analysis of transcripts in uninfected and R. typhi -infected KC167 cells. RNA was extracted from KC167 cells infected with R. typhi for 0 or 4 h, and cDNA was used in the Fruit Fly Signal Transduction PathwayFinder PCR array.

    Journal: Infection and Immunity

    Article Title: The Cat Flea ( Ctenocephalides felis) Immune Deficiency Signaling Pathway Regulates Rickettsia typhi Infection

    doi: 10.1128/IAI.00562-17

    Figure Lengend Snippet: R. typhi elicits an immune response in Drosophila cells. Shown are results of a PCR array analysis of transcripts in uninfected and R. typhi -infected KC167 cells. RNA was extracted from KC167 cells infected with R. typhi for 0 or 4 h, and cDNA was used in the Fruit Fly Signal Transduction PathwayFinder PCR array.

    Article Snippet: Four hours postinfection, RNA was isolated from KC167 cells using the Quick-RNA Mini-Prep Plus kit (Zymo Research; R1055) and cDNAs were synthesized from 200 ng of RNA using an RT2 First Strand kit (Qiagen; 330401).

    Techniques: Polymerase Chain Reaction, Infection, Transduction

    Lethal Dox Suppresses LRP1 Protein Translation through the p53-Regulated miRNAs MiR-103 and MiR-107 (A) HCT116 cells were treated with the indicated dose of Dox (nanomolar) for 24 hr, after which RNA was collected and subjected to qRT-PCR analysis. Data represent at least two biological replicates, each performed in triplicate. (B) HCT116 cells were treated with the indicated dose of Dox for 24 hr, after which cells were subjected to ChIP analysis using anti-p53 antibody and non-specific IgG. Data represent at least two biological replicates, each performed in triplicate. (C) HCT116 cells were treated with the indicated stress for 24 hr, after which vehicle, MG132 (MG), or CQ was added to the cells for an additional 8 hr. After MG or CQ treatment, lysates were collected and subjected to western blot analysis. Data represent at least two biological replicates. (D) HCT116 cells were treated with the indicated course of Dox for 24 hr, after which cells were labeled with 35 S-Met/Cys for 30 min, chased with complete DMEM for another 30 min, and then subjected to LRP1 immunoprecipitation. Data represent at least two biological replicates.

    Journal: Cell reports

    Article Title: p53 Regulates the Expression of LRP1 and Apoptosis through a Stress Intensity-Dependent MicroRNA Feedback Loop

    doi: 10.1016/j.celrep.2018.07.010

    Figure Lengend Snippet: Lethal Dox Suppresses LRP1 Protein Translation through the p53-Regulated miRNAs MiR-103 and MiR-107 (A) HCT116 cells were treated with the indicated dose of Dox (nanomolar) for 24 hr, after which RNA was collected and subjected to qRT-PCR analysis. Data represent at least two biological replicates, each performed in triplicate. (B) HCT116 cells were treated with the indicated dose of Dox for 24 hr, after which cells were subjected to ChIP analysis using anti-p53 antibody and non-specific IgG. Data represent at least two biological replicates, each performed in triplicate. (C) HCT116 cells were treated with the indicated stress for 24 hr, after which vehicle, MG132 (MG), or CQ was added to the cells for an additional 8 hr. After MG or CQ treatment, lysates were collected and subjected to western blot analysis. Data represent at least two biological replicates. (D) HCT116 cells were treated with the indicated course of Dox for 24 hr, after which cells were labeled with 35 S-Met/Cys for 30 min, chased with complete DMEM for another 30 min, and then subjected to LRP1 immunoprecipitation. Data represent at least two biological replicates.

    Article Snippet: qRT-PCR After treatment, RNA was purified from cells using the Zymo Quick RNA mini-prep kit according to the protocol recommended by the manufacturer (Zymo Research, catalog no. R1057).

    Techniques: Quantitative RT-PCR, Chromatin Immunoprecipitation, Western Blot, Labeling, Immunoprecipitation