quick dna viral kit  (Zymo Research)


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    Name:
    Quick DNA Viral Kit
    Description:
    The Quick DNA Viral Kits provide for the rapid isolation of high quality viral DNA from a wide range of biological sources A uniquely designed buffer is included for the efficient denaturation of viral particles in whole blood fresh and stored plasma serum tissue ascites cultured cells and from liquid samples DNA can be eluted with elution buffer or water and is suitable for subsequent PCR nucleotide blotting and restriction endonuclease digestion procedures
    Catalog Number:
    d3016
    Product Aliases:
    ZR Viral DNA Kit
    Price:
    None
    Applications:
    Viral DNA Purification
    Size:
    50 units
    Category:
    Life Science Reagents and Media
    Buy from Supplier


    Structured Review

    Zymo Research quick dna viral kit
    Quick DNA Viral Kit
    The Quick DNA Viral Kits provide for the rapid isolation of high quality viral DNA from a wide range of biological sources A uniquely designed buffer is included for the efficient denaturation of viral particles in whole blood fresh and stored plasma serum tissue ascites cultured cells and from liquid samples DNA can be eluted with elution buffer or water and is suitable for subsequent PCR nucleotide blotting and restriction endonuclease digestion procedures
    https://www.bioz.com/result/quick dna viral kit/product/Zymo Research
    Average 95 stars, based on 15 article reviews
    Price from $9.99 to $1999.99
    quick dna viral kit - by Bioz Stars, 2020-02
    95/100 stars

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    Related Articles

    Diagnostic Assay:

    Article Title: Alcelaphine Herpesvirus-1 (Malignant Catarrhal Fever Virus) in Wildebeest Placenta: Genetic Variation of ORF50 and A9.5 Alleles
    Article Snippet: DNA was purified from the frozen cattle buffy coat samples using the ZR Viral DNA Kit (Zymo Research Corporation, USA); from the FTA card stored wildebeest buffy coat samples using QiaAmp Mini Kit (Qiagen, Crawley, UK); and from the frozen placental samples using the Qiagen DNeasy Blood and Tissue Kit. .. Thereafter viral DNA was detected and analysed by AlHV-1-specific duplex real-time PCR [ ],[ ] (targeting the AlHV-1 ORF3 gene [ ] and the genomic β-actin gene [ ]) and nested diagnostic PCR [ ].

    Centrifugation:

    Article Title: Complete Genome Sequences of Escherichia coli Phages vB_EcoM-EP75 and vB_EcoP-EP335
    Article Snippet: After centrifugation, the supernatant was filter sterilized (0.2-µm membrane filter) and used in two consecutive rounds of plaque isolation to obtain a pure phage solution. .. Phage DNA was extracted using the Quick-DNA viral kit (Zymo Research, Irvine, CA) according to the manufacturer’s protocol.

    Amplification:

    Article Title: Bacteriophage biodistribution and infectivity from honeybee to bee larvae using a T7 phage model
    Article Snippet: Before DNA extraction, samples were supplemented with 25 ng.µL−1 of the IAC template (a 3075 bp purified amplicon originary from E. coli LacZ gene). .. Zymo Quick-DNA™ Viral Kit was used for DNA purification, with some modifications relatively to the manufacturer instructions: a 3 hours period of incubation with the supplied Lysis Buffer and the elution in 15 µL of the supplied Storage Buffer.

    Article Title: Bacteriophage biodistribution and infectivity from honeybee to bee larvae using a T7 phage model
    Article Snippet: 100 − ( ( 25 ng . µ L − 1 [ IAC ] sample ng . µ L − 1 ) / 25 ng . µ L − 1 ) ∗ 100 1 To assess the amplification efficiency of the primer pair T7 MCP another standard curve was defined: CqMCP = −3.486 × Log [T7 MCP] (ng.µL−1 )+11.467). .. In order to estimate T7 phage concentration (PFU.mL−1 ) in the treated samples, the DNA of five serial dilutions of a 108 PFU.mL−1 T7-phage suspension was purified (Zymo Quick-DNA™ Viral Kit), and used as template for qPCR analysis (T7 MCP primers).

    Quantitative RT-PCR:

    Article Title: Peroxisomes support human herpesvirus 8 latency by stabilizing the viral oncogenic protein vFLIP via the MAVS-TRAF complex
    Article Snippet: HHV-8 replication assay For determination of encapsidated HHV-8 genome copy number from the culture supernatant, viral DNA was purified using Quick-DNA™ Viral Kit (Zymo Research) following pretreatment of virus suspension with DNase I (New England BioLabs) at 37°C overnight. .. RT-qPCR was performed using ABI PRISM 7500 system as described above with the LANA primers ( ).

    Article Title: Peroxisomes support human herpesvirus 8 latency by stabilizing the viral oncogenic protein vFLIP via the MAVS-TRAF complex
    Article Snippet: For determination of encapsidated HHV-8 genome copy number from the culture supernatant, viral DNA was purified using Quick-DNA™ Viral Kit (Zymo Research) following pretreatment of virus suspension with DNase I (New England BioLabs) at 37°C overnight. .. RT-qPCR was performed using ABI PRISM 7500 system as described above with the LANA primers ( ).

    Article Title: Activation of NIX-mediated mitophagy by an interferon regulatory factor homologue of human herpesvirus
    Article Snippet: HHV-8 replication assays For determination of encapsidated HHV-8 genome copy number, viral DNA was purified using Quick-DNA™ Viral Kit (Zymo Research) following pretreatment of the culture supernatants containing HHV-8 virions with DNase I (New England BioLabs) at 37 ℃ overnight. .. RT-qPCR was performed as described above with LANA primers (Supplementary Table ).

    Real-time Polymerase Chain Reaction:

    Article Title: Bacteriophage biodistribution and infectivity from honeybee to bee larvae using a T7 phage model
    Article Snippet: DNA extraction and purification from biological samples T7 phage DNA present in the above treated samples was assessed by qPCR in a CFX96 thermal cycler (Bio-Rad) and for that two different genes with published sequences were targeted in separate reactions: the T7 major capsid protein (MCP) gene for phage detection and the E. coli LacZ gene for the internal amplification control (IAC), used to avoid false negative results and to assess phage DNA purification efficiency. .. Zymo Quick-DNA™ Viral Kit was used for DNA purification, with some modifications relatively to the manufacturer instructions: a 3 hours period of incubation with the supplied Lysis Buffer and the elution in 15 µL of the supplied Storage Buffer.

    Article Title: Distribution of Porcine Cytomegalovirus in Infected Donor Pigs and in Baboon Recipients of Pig Heart Transplantation
    Article Snippet: Briefly, DNA was extracted from sera and blood of the pigs using two DNA extraction kits, a DNeasy Blood and Tissue kit (Qiagen GmbH, Hilden, Germany) and a ZR viral DNA kit (Zymo Research Corp., Irvine, CA, USA). .. To screen for PCMV, a real-time PCR with a detection limit of two to five copies was performed using a SensiFast probe no ROX one-step kit, according to supplier recommendations (Bioline GmbH, Germany).

    Article Title: Bacteriophage biodistribution and infectivity from honeybee to bee larvae using a T7 phage model
    Article Snippet: .. In order to estimate T7 phage concentration (PFU.mL−1 ) in the treated samples, the DNA of five serial dilutions of a 108 PFU.mL−1 T7-phage suspension was purified (Zymo Quick-DNA™ Viral Kit), and used as template for qPCR analysis (T7 MCP primers). ..

    Article Title: Alcelaphine Herpesvirus-1 (Malignant Catarrhal Fever Virus) in Wildebeest Placenta: Genetic Variation of ORF50 and A9.5 Alleles
    Article Snippet: DNA was purified from the frozen cattle buffy coat samples using the ZR Viral DNA Kit (Zymo Research Corporation, USA); from the FTA card stored wildebeest buffy coat samples using QiaAmp Mini Kit (Qiagen, Crawley, UK); and from the frozen placental samples using the Qiagen DNeasy Blood and Tissue Kit. .. Thereafter viral DNA was detected and analysed by AlHV-1-specific duplex real-time PCR [ ],[ ] (targeting the AlHV-1 ORF3 gene [ ] and the genomic β-actin gene [ ]) and nested diagnostic PCR [ ].

    Incubation:

    Article Title: Bacteriophage biodistribution and infectivity from honeybee to bee larvae using a T7 phage model
    Article Snippet: .. Zymo Quick-DNA™ Viral Kit was used for DNA purification, with some modifications relatively to the manufacturer instructions: a 3 hours period of incubation with the supplied Lysis Buffer and the elution in 15 µL of the supplied Storage Buffer. .. T7 phage quantification in bees and larva In order to quantify the IAC concentration present in the purified DNA, a standard curve was obtained (CqIAC = −3.781 × Log [IAC] (ng.µL−1 ) + 8.369 (Cq = quantification cycle)) using several concentrations of the LacZ amplicon (5.5, 0.55, 0.11, 0.055 ng.µL−1 ).

    Article Title: Complete Genome Sequences of Escherichia coli Phages vB_EcoM-EP75 and vB_EcoP-EP335
    Article Snippet: Single plaques were picked using a Pasteur pipette and transferred to 300 µl of sodium chloride-magnesium sulfate (SM) buffer (100 mM NaCl, 8 mM MgSO4 , 50 mM Tris-HCl [pH 7.4]), followed by a 2-h incubation at 30°C. .. Phage DNA was extracted using the Quick-DNA viral kit (Zymo Research, Irvine, CA) according to the manufacturer’s protocol.

    Expressing:

    Article Title: Activation of NIX-mediated mitophagy by an interferon regulatory factor homologue of human herpesvirus
    Article Snippet: HHV-8 replication assays For determination of encapsidated HHV-8 genome copy number, viral DNA was purified using Quick-DNA™ Viral Kit (Zymo Research) following pretreatment of the culture supernatants containing HHV-8 virions with DNase I (New England BioLabs) at 37 ℃ overnight. .. Also, the expression levels of lytic antigens including ORF45 and K8.1 were determined by immunoblotting.

    Electron Microscopy:

    Article Title: Bacteriophage Therapy Testing Against Shigella flexneri in a Novel Human Intestinal Organoid-Derived Infection Model
    Article Snippet: For visualization of Φ2457T, PEG-purified samples were loaded onto Formvar/carbon-coated nickel grids (FCF200-Ni, Electron Microscopy Sciences, Hatfield, PA) and stained with 2% uranyl acetate for transmission electron microscopy (TEM) with an FEI Tecnai Spirit Transmission Electron Microscope operating at 80 kV. .. For sequencing of the phage genome, purified phage preparations were treated with DNase and RNase to remove bacterial nucleic acid contamination, and then DNA was extracted using the ZR Viral DNA Kit with IC-XL columns (Zymo Research, Irvine, CA).

    Transferring:

    Article Title: Complete Genome Sequences of Escherichia coli Phages vB_EcoM-EP75 and vB_EcoP-EP335
    Article Snippet: Single plaques were picked using a Pasteur pipette and transferred to 300 µl of sodium chloride-magnesium sulfate (SM) buffer (100 mM NaCl, 8 mM MgSO4 , 50 mM Tris-HCl [pH 7.4]), followed by a 2-h incubation at 30°C. .. Phage DNA was extracted using the Quick-DNA viral kit (Zymo Research, Irvine, CA) according to the manufacturer’s protocol.

    Generated:

    Article Title: Bacteriophage biodistribution and infectivity from honeybee to bee larvae using a T7 phage model
    Article Snippet: For that 5 µL of SsoFast™ EvaGreen (BioRad), 2 µL of DNA template, 1 µL of a 5 µM LacZ (IAC) primer mix and HyPureTM Molecular Biology Grade water (GE, Healthcare) up to 10 µL were mixed and run for 3 minutes at 95 °C followed by 40 cycles of 10 seconds at 95 °C, 10 seconds at 58 °C, and 10 seconds at 65 °C (the melt curve was generated by heating from 65 to 95 °C with increments of 1 °C, 5 seconds dwell time). .. In order to estimate T7 phage concentration (PFU.mL−1 ) in the treated samples, the DNA of five serial dilutions of a 108 PFU.mL−1 T7-phage suspension was purified (Zymo Quick-DNA™ Viral Kit), and used as template for qPCR analysis (T7 MCP primers).

    Article Title: Complete Genome Sequences of Escherichia coli Phages vB_EcoM-EP75 and vB_EcoP-EP335
    Article Snippet: Phage DNA was extracted using the Quick-DNA viral kit (Zymo Research, Irvine, CA) according to the manufacturer’s protocol. .. The resulting paired-end sequence reads were generated using an Illumina HiSeq 2500 system.

    DNA Sequencing:

    Article Title: Genetic Variability of Myxoma Virus Genomes
    Article Snippet: Paragraph title: DNA sequencing and assembly. ... In addition, viral DNA was prepared (ZR Viral DNA kit; Zymo Research, Irvine, CA, USA) and sent for Sanger sequencing to close the remaining gaps.

    Transmission Assay:

    Article Title: Bacteriophage Therapy Testing Against Shigella flexneri in a Novel Human Intestinal Organoid-Derived Infection Model
    Article Snippet: For visualization of Φ2457T, PEG-purified samples were loaded onto Formvar/carbon-coated nickel grids (FCF200-Ni, Electron Microscopy Sciences, Hatfield, PA) and stained with 2% uranyl acetate for transmission electron microscopy (TEM) with an FEI Tecnai Spirit Transmission Electron Microscope operating at 80 kV. .. For sequencing of the phage genome, purified phage preparations were treated with DNase and RNase to remove bacterial nucleic acid contamination, and then DNA was extracted using the ZR Viral DNA Kit with IC-XL columns (Zymo Research, Irvine, CA).

    Sequencing:

    Article Title: Genetic Variability of Myxoma Virus Genomes
    Article Snippet: .. In addition, viral DNA was prepared (ZR Viral DNA kit; Zymo Research, Irvine, CA, USA) and sent for Sanger sequencing to close the remaining gaps. .. Sequence editing was performed by using the GAP4 program in the Staden software package ( ).

    Article Title: Bacteriophage Therapy Testing Against Shigella flexneri in a Novel Human Intestinal Organoid-Derived Infection Model
    Article Snippet: .. For sequencing of the phage genome, purified phage preparations were treated with DNase and RNase to remove bacterial nucleic acid contamination, and then DNA was extracted using the ZR Viral DNA Kit with IC-XL columns (Zymo Research, Irvine, CA). .. Purified phage genomic DNA was submitted to the MIT BioMicro Center (Cambridge, MA) for Nextera library preparation for 150 bp paired-end read sequencing on a single Illumina MiSeq lane, yielding approximately 9.7 million paired-end reads.

    Article Title: Complete Genome Sequences of Escherichia coli Phages vB_EcoM-EP75 and vB_EcoP-EP335
    Article Snippet: Phage DNA was extracted using the Quick-DNA viral kit (Zymo Research, Irvine, CA) according to the manufacturer’s protocol. .. The resulting paired-end sequence reads were generated using an Illumina HiSeq 2500 system.

    Transmission Electron Microscopy:

    Article Title: Bacteriophage Therapy Testing Against Shigella flexneri in a Novel Human Intestinal Organoid-Derived Infection Model
    Article Snippet: For visualization of Φ2457T, PEG-purified samples were loaded onto Formvar/carbon-coated nickel grids (FCF200-Ni, Electron Microscopy Sciences, Hatfield, PA) and stained with 2% uranyl acetate for transmission electron microscopy (TEM) with an FEI Tecnai Spirit Transmission Electron Microscope operating at 80 kV. .. For sequencing of the phage genome, purified phage preparations were treated with DNase and RNase to remove bacterial nucleic acid contamination, and then DNA was extracted using the ZR Viral DNA Kit with IC-XL columns (Zymo Research, Irvine, CA).

    DNA Extraction:

    Article Title: Bacteriophage biodistribution and infectivity from honeybee to bee larvae using a T7 phage model
    Article Snippet: Paragraph title: DNA extraction and purification from biological samples ... Zymo Quick-DNA™ Viral Kit was used for DNA purification, with some modifications relatively to the manufacturer instructions: a 3 hours period of incubation with the supplied Lysis Buffer and the elution in 15 µL of the supplied Storage Buffer.

    Article Title: Distribution of Porcine Cytomegalovirus in Infected Donor Pigs and in Baboon Recipients of Pig Heart Transplantation
    Article Snippet: .. Briefly, DNA was extracted from sera and blood of the pigs using two DNA extraction kits, a DNeasy Blood and Tissue kit (Qiagen GmbH, Hilden, Germany) and a ZR viral DNA kit (Zymo Research Corp., Irvine, CA, USA). ..

    Article Title: Bacteriophage biodistribution and infectivity from honeybee to bee larvae using a T7 phage model
    Article Snippet: The assessment of the DNA extraction efficacy was obtained through equation ( ) that was used to normalise T7 phage concentration obtained from biological samples. .. In order to estimate T7 phage concentration (PFU.mL−1 ) in the treated samples, the DNA of five serial dilutions of a 108 PFU.mL−1 T7-phage suspension was purified (Zymo Quick-DNA™ Viral Kit), and used as template for qPCR analysis (T7 MCP primers).

    Isolation:

    Article Title: Distribution of Porcine Cytomegalovirus in Infected Donor Pigs and in Baboon Recipients of Pig Heart Transplantation
    Article Snippet: Briefly, DNA was extracted from sera and blood of the pigs using two DNA extraction kits, a DNeasy Blood and Tissue kit (Qiagen GmbH, Hilden, Germany) and a ZR viral DNA kit (Zymo Research Corp., Irvine, CA, USA). .. DNA was isolated from organs using the DNeasy Blood & Tissue Kit.

    Article Title: Bacteriophage Therapy Testing Against Shigella flexneri in a Novel Human Intestinal Organoid-Derived Infection Model
    Article Snippet: Paragraph title: Isolation of Bacteriophage Against S. flexneri ... For sequencing of the phage genome, purified phage preparations were treated with DNase and RNase to remove bacterial nucleic acid contamination, and then DNA was extracted using the ZR Viral DNA Kit with IC-XL columns (Zymo Research, Irvine, CA).

    Article Title: Complete Genome Sequences of Escherichia coli Phages vB_EcoM-EP75 and vB_EcoP-EP335
    Article Snippet: After centrifugation, the supernatant was filter sterilized (0.2-µm membrane filter) and used in two consecutive rounds of plaque isolation to obtain a pure phage solution. .. Phage DNA was extracted using the Quick-DNA viral kit (Zymo Research, Irvine, CA) according to the manufacturer’s protocol.

    Microscopy:

    Article Title: Bacteriophage Therapy Testing Against Shigella flexneri in a Novel Human Intestinal Organoid-Derived Infection Model
    Article Snippet: For visualization of Φ2457T, PEG-purified samples were loaded onto Formvar/carbon-coated nickel grids (FCF200-Ni, Electron Microscopy Sciences, Hatfield, PA) and stained with 2% uranyl acetate for transmission electron microscopy (TEM) with an FEI Tecnai Spirit Transmission Electron Microscope operating at 80 kV. .. For sequencing of the phage genome, purified phage preparations were treated with DNase and RNase to remove bacterial nucleic acid contamination, and then DNA was extracted using the ZR Viral DNA Kit with IC-XL columns (Zymo Research, Irvine, CA).

    Purification:

    Article Title: Peroxisomes support human herpesvirus 8 latency by stabilizing the viral oncogenic protein vFLIP via the MAVS-TRAF complex
    Article Snippet: .. HHV-8 replication assay For determination of encapsidated HHV-8 genome copy number from the culture supernatant, viral DNA was purified using Quick-DNA™ Viral Kit (Zymo Research) following pretreatment of virus suspension with DNase I (New England BioLabs) at 37°C overnight. .. RT-qPCR was performed using ABI PRISM 7500 system as described above with the LANA primers ( ).

    Article Title: Bacteriophage biodistribution and infectivity from honeybee to bee larvae using a T7 phage model
    Article Snippet: Paragraph title: DNA extraction and purification from biological samples ... Zymo Quick-DNA™ Viral Kit was used for DNA purification, with some modifications relatively to the manufacturer instructions: a 3 hours period of incubation with the supplied Lysis Buffer and the elution in 15 µL of the supplied Storage Buffer.

    Article Title: Bacteriophage Therapy Testing Against Shigella flexneri in a Novel Human Intestinal Organoid-Derived Infection Model
    Article Snippet: .. For sequencing of the phage genome, purified phage preparations were treated with DNase and RNase to remove bacterial nucleic acid contamination, and then DNA was extracted using the ZR Viral DNA Kit with IC-XL columns (Zymo Research, Irvine, CA). .. Purified phage genomic DNA was submitted to the MIT BioMicro Center (Cambridge, MA) for Nextera library preparation for 150 bp paired-end read sequencing on a single Illumina MiSeq lane, yielding approximately 9.7 million paired-end reads.

    Article Title: Bacteriophage biodistribution and infectivity from honeybee to bee larvae using a T7 phage model
    Article Snippet: .. In order to estimate T7 phage concentration (PFU.mL−1 ) in the treated samples, the DNA of five serial dilutions of a 108 PFU.mL−1 T7-phage suspension was purified (Zymo Quick-DNA™ Viral Kit), and used as template for qPCR analysis (T7 MCP primers). ..

    Article Title: Complete Genome Sequences of Escherichia coli Phages vB_EcoM-EP75 and vB_EcoP-EP335
    Article Snippet: Phages were purified using CsCl gradient centrifugation ( ). .. Phage DNA was extracted using the Quick-DNA viral kit (Zymo Research, Irvine, CA) according to the manufacturer’s protocol.

    Article Title: Peroxisomes support human herpesvirus 8 latency by stabilizing the viral oncogenic protein vFLIP via the MAVS-TRAF complex
    Article Snippet: .. For determination of encapsidated HHV-8 genome copy number from the culture supernatant, viral DNA was purified using Quick-DNA™ Viral Kit (Zymo Research) following pretreatment of virus suspension with DNase I (New England BioLabs) at 37°C overnight. .. RT-qPCR was performed using ABI PRISM 7500 system as described above with the LANA primers ( ).

    Article Title: Alcelaphine Herpesvirus-1 (Malignant Catarrhal Fever Virus) in Wildebeest Placenta: Genetic Variation of ORF50 and A9.5 Alleles
    Article Snippet: .. DNA was purified from the frozen cattle buffy coat samples using the ZR Viral DNA Kit (Zymo Research Corporation, USA); from the FTA card stored wildebeest buffy coat samples using QiaAmp Mini Kit (Qiagen, Crawley, UK); and from the frozen placental samples using the Qiagen DNeasy Blood and Tissue Kit. .. Thereafter viral DNA was detected and analysed by AlHV-1-specific duplex real-time PCR [ ],[ ] (targeting the AlHV-1 ORF3 gene [ ] and the genomic β-actin gene [ ]) and nested diagnostic PCR [ ].

    Article Title: Activation of NIX-mediated mitophagy by an interferon regulatory factor homologue of human herpesvirus
    Article Snippet: .. HHV-8 replication assays For determination of encapsidated HHV-8 genome copy number, viral DNA was purified using Quick-DNA™ Viral Kit (Zymo Research) following pretreatment of the culture supernatants containing HHV-8 virions with DNase I (New England BioLabs) at 37 ℃ overnight. .. RT-qPCR was performed as described above with LANA primers (Supplementary Table ).

    Polymerase Chain Reaction:

    Article Title: Distribution of Porcine Cytomegalovirus in Infected Donor Pigs and in Baboon Recipients of Pig Heart Transplantation
    Article Snippet: Paragraph title: 2.3. Testing for PCMV, HEV and BaCMV, PCR Methods ... Briefly, DNA was extracted from sera and blood of the pigs using two DNA extraction kits, a DNeasy Blood and Tissue kit (Qiagen GmbH, Hilden, Germany) and a ZR viral DNA kit (Zymo Research Corp., Irvine, CA, USA).

    Article Title: The efficacy of alcelaphine herpesvirus-1 (AlHV-1) immunization with the adjuvants Emulsigen® and the monomeric TLR5 ligand FliC in zebu cattle against AlHV-1 malignant catarrhal fever induced by experimental virus challenge
    Article Snippet: 2.6 Detection of AlHV-1 in blood Viral DNA was extracted from the frozen buffy coat samples at the Nelson Mandela African Institution of Science and Technology (Tanzania) using the ZR Viral DNA Kit™ (Zymo Research Corporation, USA) according to the manufacturer’s instructions. .. Briefly, following a first round of AlHV-1 specific PCR, performed using AHV-POL1 (5′-ggctcataatctatgctactccac-3′) and AHV-POL2 (5′-attctccacaaactgttttgt-3′) primers, a 2 μl aliquot was used for a second hemi-nested round of PCR performed using AHV-POL internal forward primer (5′-ccaaaatgaagaccatctta-3′), and the first-round POL2 as reverse primer.

    Article Title: Alcelaphine Herpesvirus-1 (Malignant Catarrhal Fever Virus) in Wildebeest Placenta: Genetic Variation of ORF50 and A9.5 Alleles
    Article Snippet: Detection of viral DNA The presence of AlHV-1 was determined by PCR analysis of DNA extracted from buffy coat cells and placenta tissue samples. .. DNA was purified from the frozen cattle buffy coat samples using the ZR Viral DNA Kit (Zymo Research Corporation, USA); from the FTA card stored wildebeest buffy coat samples using QiaAmp Mini Kit (Qiagen, Crawley, UK); and from the frozen placental samples using the Qiagen DNeasy Blood and Tissue Kit.

    Article Title: A field vaccine trial in Tanzania demonstrates partial protection against malignant catarrhal fever in cattle
    Article Snippet: 2.6 Detection of viral DNA in blood Viral DNA was extracted from frozen buffy coat samples using the ZR Viral DNA Kit™(Zymo Research Coporation, USA) according to the manufacturer's instructions and was assayed by nested PCR as described previously . .. Animals were classified as AlHV-1 positive if one or more PCR analyses during the challenge phase were positive.

    Staining:

    Article Title: Bacteriophage Therapy Testing Against Shigella flexneri in a Novel Human Intestinal Organoid-Derived Infection Model
    Article Snippet: For visualization of Φ2457T, PEG-purified samples were loaded onto Formvar/carbon-coated nickel grids (FCF200-Ni, Electron Microscopy Sciences, Hatfield, PA) and stained with 2% uranyl acetate for transmission electron microscopy (TEM) with an FEI Tecnai Spirit Transmission Electron Microscope operating at 80 kV. .. For sequencing of the phage genome, purified phage preparations were treated with DNase and RNase to remove bacterial nucleic acid contamination, and then DNA was extracted using the ZR Viral DNA Kit with IC-XL columns (Zymo Research, Irvine, CA).

    Nested PCR:

    Article Title: The efficacy of alcelaphine herpesvirus-1 (AlHV-1) immunization with the adjuvants Emulsigen® and the monomeric TLR5 ligand FliC in zebu cattle against AlHV-1 malignant catarrhal fever induced by experimental virus challenge
    Article Snippet: 2.6 Detection of AlHV-1 in blood Viral DNA was extracted from the frozen buffy coat samples at the Nelson Mandela African Institution of Science and Technology (Tanzania) using the ZR Viral DNA Kit™ (Zymo Research Corporation, USA) according to the manufacturer’s instructions. .. Viral DNA was assayed by a sensitive, nested PCR as described previously ( ) and validated for use in the UK and Tanzania laboratories.

    Article Title: A field vaccine trial in Tanzania demonstrates partial protection against malignant catarrhal fever in cattle
    Article Snippet: .. 2.6 Detection of viral DNA in blood Viral DNA was extracted from frozen buffy coat samples using the ZR Viral DNA Kit™(Zymo Research Coporation, USA) according to the manufacturer's instructions and was assayed by nested PCR as described previously . .. Animals were classified as AlHV-1 positive if one or more PCR analyses during the challenge phase were positive.

    Shotgun Sequencing:

    Article Title: Genetic Variability of Myxoma Virus Genomes
    Article Snippet: Whole-genome shotgun sequencing of the MYXV genomes was performed by using a 454 GS-FLX system (Roche 454 Life Science, Mannheim, Germany). .. In addition, viral DNA was prepared (ZR Viral DNA kit; Zymo Research, Irvine, CA, USA) and sent for Sanger sequencing to close the remaining gaps.

    Software:

    Article Title: Genetic Variability of Myxoma Virus Genomes
    Article Snippet: The initial assembly was performed with Roche Newbler 2.3 FLX assembler software (454 Life Sciences, Roche Applied Science, Branford, CT), using MYXV Lausanne as the reference genome. .. In addition, viral DNA was prepared (ZR Viral DNA kit; Zymo Research, Irvine, CA, USA) and sent for Sanger sequencing to close the remaining gaps.

    Article Title: Bacteriophage biodistribution and infectivity from honeybee to bee larvae using a T7 phage model
    Article Snippet: Primers (Table ) were designed using SnapGene™ software (version 1.1.3) ( www.snapgene.com ). .. Zymo Quick-DNA™ Viral Kit was used for DNA purification, with some modifications relatively to the manufacturer instructions: a 3 hours period of incubation with the supplied Lysis Buffer and the elution in 15 µL of the supplied Storage Buffer.

    Negative Control:

    Article Title: Bacteriophage biodistribution and infectivity from honeybee to bee larvae using a T7 phage model
    Article Snippet: In order to estimate T7 phage concentration (PFU.mL−1 ) in the treated samples, the DNA of five serial dilutions of a 108 PFU.mL−1 T7-phage suspension was purified (Zymo Quick-DNA™ Viral Kit), and used as template for qPCR analysis (T7 MCP primers). .. A negative control using HyPureTM water was included in each reaction and three replicates of each condition were analysed in all the assays.

    Concentration Assay:

    Article Title: Bacteriophage biodistribution and infectivity from honeybee to bee larvae using a T7 phage model
    Article Snippet: .. In order to estimate T7 phage concentration (PFU.mL−1 ) in the treated samples, the DNA of five serial dilutions of a 108 PFU.mL−1 T7-phage suspension was purified (Zymo Quick-DNA™ Viral Kit), and used as template for qPCR analysis (T7 MCP primers). ..

    DNA Purification:

    Article Title: Bacteriophage biodistribution and infectivity from honeybee to bee larvae using a T7 phage model
    Article Snippet: .. Zymo Quick-DNA™ Viral Kit was used for DNA purification, with some modifications relatively to the manufacturer instructions: a 3 hours period of incubation with the supplied Lysis Buffer and the elution in 15 µL of the supplied Storage Buffer. .. T7 phage quantification in bees and larva In order to quantify the IAC concentration present in the purified DNA, a standard curve was obtained (CqIAC = −3.781 × Log [IAC] (ng.µL−1 ) + 8.369 (Cq = quantification cycle)) using several concentrations of the LacZ amplicon (5.5, 0.55, 0.11, 0.055 ng.µL−1 ).

    Lysis:

    Article Title: Bacteriophage biodistribution and infectivity from honeybee to bee larvae using a T7 phage model
    Article Snippet: .. Zymo Quick-DNA™ Viral Kit was used for DNA purification, with some modifications relatively to the manufacturer instructions: a 3 hours period of incubation with the supplied Lysis Buffer and the elution in 15 µL of the supplied Storage Buffer. .. T7 phage quantification in bees and larva In order to quantify the IAC concentration present in the purified DNA, a standard curve was obtained (CqIAC = −3.781 × Log [IAC] (ng.µL−1 ) + 8.369 (Cq = quantification cycle)) using several concentrations of the LacZ amplicon (5.5, 0.55, 0.11, 0.055 ng.µL−1 ).

    Gradient Centrifugation:

    Article Title: Complete Genome Sequences of Escherichia coli Phages vB_EcoM-EP75 and vB_EcoP-EP335
    Article Snippet: Phages were purified using CsCl gradient centrifugation ( ). .. Phage DNA was extracted using the Quick-DNA viral kit (Zymo Research, Irvine, CA) according to the manufacturer’s protocol.

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  • 95
    Zymo Research quick dna viral kit
    Quick Dna Viral Kit, supplied by Zymo Research, used in various techniques. Bioz Stars score: 95/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quick dna viral kit/product/Zymo Research
    Average 95 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    quick dna viral kit - by Bioz Stars, 2020-02
    95/100 stars
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