zr plant seed dna miniprep kit  (Zymo Research)


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    Name:
    Quick DNA Plant Seed Miniprep Kit
    Description:
    The Quick DNA Plant Seed Miniprep Kit is designed for the simple rapid isolation of inhibitor free PCR quality DNA from a variety of plant sample sources including leaves stems buds flowers fruit seeds etc The procedure is easy and can be completed in minutes plant samples are rapidly and efficiently lysed by bead beating with our state of the art ultra high density BashingBeads Polysaccharides lipids and polyphenols tannins are removed from the DNA using our Zymo Spin column technology The eluted DNA is ideal for downstream molecular based applications including PCR arrays etc
    Catalog Number:
    d6020
    Product Aliases:
    ZR Plant/Seed DNA MiniPrep
    Price:
    None
    Applications:
    DNA Purification
    Size:
    50 units
    Category:
    Life Science Reagents and Media
    Buy from Supplier


    Structured Review

    Zymo Research zr plant seed dna miniprep kit
    Quick DNA Plant Seed Miniprep Kit
    The Quick DNA Plant Seed Miniprep Kit is designed for the simple rapid isolation of inhibitor free PCR quality DNA from a variety of plant sample sources including leaves stems buds flowers fruit seeds etc The procedure is easy and can be completed in minutes plant samples are rapidly and efficiently lysed by bead beating with our state of the art ultra high density BashingBeads Polysaccharides lipids and polyphenols tannins are removed from the DNA using our Zymo Spin column technology The eluted DNA is ideal for downstream molecular based applications including PCR arrays etc
    https://www.bioz.com/result/zr plant seed dna miniprep kit/product/Zymo Research
    Average 95 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    zr plant seed dna miniprep kit - by Bioz Stars, 2020-02
    95/100 stars

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    Related Articles

    Amplification:

    Article Title: A metagenomic study of DNA viruses from samples of local varieties of common bean in Kenya
    Article Snippet: DNA extraction and library preparation of the host plant DNA extraction was carried on 12 viral leaves with chlorosis symptomatic leaves using the Quick-DNA plant/seed miniprep kit (Zymo Cat log D6020). .. Rolling circle amplification was then carried out on each of the DNA samples using the TempliPhi™ 100 amplification kit as described by the manufacturer (GE).

    Article Title: Genome-wide analysis of cytosine DNA methylation revealed salicylic acid promotes defense pathways over seedling development in pearl millet
    Article Snippet: DNA was extracted from leaves of 20 seedlings (4 lines, 5 SA doses) with four replicates using ZR plant/seed DNA miniprep (Zymo Research, Cat No. D6020) following the company protocol and an alcohol isoamyl-chloroform step was included to increase DNA yield and quality. .. The extracted DNA was subjected to a Methylation-Sensitive Amplified Polymorphism (MSAP) analysis using two isoschizomeres MspI and HpaII targeting the CCGG motifs, and EcoRI targeting GAATTC sites.

    Article Title: Enhanced Bacterial Wilt Resistance in Potato Through Expression of Arabidopsis EFR and Introgression of Quantitative Resistance from Solanum commersonii
    Article Snippet: Genomic DNA was isolated using the ZR Plant/Seed DNA MiniPrep Kit (Zymo Research) following manufacturer’s instructions. .. The PCR mix for amplification contained Taq Buffer 1× (Thermo), MgCl2 2.5 μM, dTNPs 0.5 μM, primers 0.4 μM, and Taq polymerase 1U (Invitrogen) in a final volume of 20 μL.

    Construct:

    Article Title: Species Composition and Trichothecene Genotype Profiling of Fusarium Field Isolates Recovered from Wheat in Poland
    Article Snippet: Total genomic DNA was extracted with the use of the Quick-DNA Plant/Seed Miniprep Kit (Zymo Research, Irvine, CA, USA) according to the manufacturer’s protocol. .. Genome libraries were constructed using a TruSeq DNA PCR-free library preparation kit (Illumina, San Diego, CA, USA).

    Electrophoresis:

    Article Title: Enhanced Bacterial Wilt Resistance in Potato Through Expression of Arabidopsis EFR and Introgression of Quantitative Resistance from Solanum commersonii
    Article Snippet: Genomic DNA was isolated using the ZR Plant/Seed DNA MiniPrep Kit (Zymo Research) following manufacturer’s instructions. .. The cycling conditions were: 94°C 3 min (initial denaturation), 94°C for 30 s (denaturation), 60°C for 40 s (annealing), 72°C for 40 s (extension) for 35 cycles, with a final extension at 72°C for 2 min. AtEFR internal primers were used ( Table ) and PCR products were analyzed by electrophoresis in 1–2% agarose gels.

    Quantitation Assay:

    Article Title: Putative Nitrogen-Fixing Bacteria Associated With the Rhizosphere and Root Endosphere of Wheat Plants Grown in an Andisol From Southern Chile
    Article Snippet: Root endosphere total DNA was extracted from 0.25 mL of surface sterile root homogenate using Quick-DNA™ Plant/Seed Miniprep Kit (Zymo Research Corp.), according to manufacturer instructions. .. The quantitation of nif H genes was done by using the primer set nifH-g1-forB (5′-GGT TGT GAC CCG AAA GCT GA-3′) and nifH-g1-rev (5′-GCG TAC ATG GCC ATC ATC TC-3′) (Bürgmann et al., ).

    Transformation Assay:

    Article Title: Enhanced Bacterial Wilt Resistance in Potato Through Expression of Arabidopsis EFR and Introgression of Quantitative Resistance from Solanum commersonii
    Article Snippet: The presence of the AtEFR gene in transformed potato lines of both genotypes was confirmed by PCR. .. Genomic DNA was isolated using the ZR Plant/Seed DNA MiniPrep Kit (Zymo Research) following manufacturer’s instructions.

    Sterility:

    Article Title: Putative Nitrogen-Fixing Bacteria Associated With the Rhizosphere and Root Endosphere of Wheat Plants Grown in an Andisol From Southern Chile
    Article Snippet: To verify sterility of rhizoplane samples, SDW from the third rinse was collected and processed as a sample for sterility control purposes. .. Root endosphere total DNA was extracted from 0.25 mL of surface sterile root homogenate using Quick-DNA™ Plant/Seed Miniprep Kit (Zymo Research Corp.), according to manufacturer instructions.

    Sequencing:

    Article Title: Comparative Analysis of Four Calypogeia Species Revealed Unexpected Change in Evolutionarily-Stable Liverwort Mitogenomes
    Article Snippet: .. Genome Sequencing, Assembly and Annotation Total genomic liverwort DNA was extracted using ZR Plant/Seed DNA MiniPrepTM kit (Zymo Research Corp., Irvine, CA, USA) following the manufacturer’s recommendations. .. The DNA libraries of Calypogeia integristipula , Calypogeia fissa ssp. fissa and Calypogeia suecica were sequenced using the Illumina HiSeq 2000 platform (Illumina, San Diego, CA, USA) to generate 100 bp paired-end reads, and the DNA library of Calypogeia arguta was sequenced using HiSeqX (Illumina) to generate 150 bp paired-end reads ( ).

    Article Title: Filamentous calcareous alga provides substrate for coral-competitive macroalgae in the degraded lagoon of Dongsha Atoll, Taiwan
    Article Snippet: For DNA barcoding, macroalgae samples were preserved in silica gel after collection, and the total genomic DNA of samples was extracted with Quick-DNA Plant/Seed Miniprep Kit (Zymo Research Co., USA). .. Sequence similarities of > 98% were considered for species identification.

    Article Title: Widespread selection and gene flow shape the genomic landscape during a radiation of monkeyflowers
    Article Snippet: Genome assembly We used a combination of short-read Illumina and long-read SMRT sequencing to assemble the genome of a single individual from the red ecotype of M . aurantiacus subspecies puniceus (population UCSD; ). .. Genomic DNA was isolated from leaf tissue using either ZR plant/seed DNA miniprep kits (Zymo Research, Irvine, CA) or GeneJet Plant Genomic DNA purification kits (Thermo Fisher, Waltham, MA).

    Generated:

    Article Title: Filamentous calcareous alga provides substrate for coral-competitive macroalgae in the degraded lagoon of Dongsha Atoll, Taiwan
    Article Snippet: For DNA barcoding, macroalgae samples were preserved in silica gel after collection, and the total genomic DNA of samples was extracted with Quick-DNA Plant/Seed Miniprep Kit (Zymo Research Co., USA). .. The newly generated sequences were deposited in GenBank and searched using BLASTn against the GenBank database ( and Tables).

    Article Title: Widespread selection and gene flow shape the genomic landscape during a radiation of monkeyflowers
    Article Snippet: Genomic DNA was isolated from leaf tissue using either ZR plant/seed DNA miniprep kits (Zymo Research, Irvine, CA) or GeneJet Plant Genomic DNA purification kits (Thermo Fisher, Waltham, MA). .. Illumina libraries were generated following the Allpaths-LG assembly pipeline [ ], which included a single fragment library with average 180 bp insert size and 3 mate-pair libraries (average insert sizes: 3.5–5 kb, 5–7 kb, and 7–13 kb).

    other:

    Article Title: Potential of Transcript Editing Across Mitogenomes of Early Land Plants Shows Novel and Familiar Trends
    Article Snippet: Material Total genomic DNA was extracted using ZR Plant/Seed DNA MiniPrepTM kit (Zymo Research Corp., Irvine, CA, USA) according to the manufacturer’s protocol from fresh tissue or herbarium specimens.

    DNA Sequencing:

    Article Title: Species Composition and Trichothecene Genotype Profiling of Fusarium Field Isolates Recovered from Wheat in Poland
    Article Snippet: Paragraph title: 4.4. DNA Sequencing, Assembly, and Annotation of Tri Core Clusters ... Total genomic DNA was extracted with the use of the Quick-DNA Plant/Seed Miniprep Kit (Zymo Research, Irvine, CA, USA) according to the manufacturer’s protocol.

    Polymerase Chain Reaction:

    Article Title: A metagenomic study of DNA viruses from samples of local varieties of common bean in Kenya
    Article Snippet: DNA extraction and library preparation of the host plant DNA extraction was carried on 12 viral leaves with chlorosis symptomatic leaves using the Quick-DNA plant/seed miniprep kit (Zymo Cat log D6020). .. Samples were dual-indexed with unique barcodes before a PCR enrichment step.

    Article Title: Species Composition and Trichothecene Genotype Profiling of Fusarium Field Isolates Recovered from Wheat in Poland
    Article Snippet: Total genomic DNA was extracted with the use of the Quick-DNA Plant/Seed Miniprep Kit (Zymo Research, Irvine, CA, USA) according to the manufacturer’s protocol. .. Genome libraries were constructed using a TruSeq DNA PCR-free library preparation kit (Illumina, San Diego, CA, USA).

    Article Title: Putative Nitrogen-Fixing Bacteria Associated With the Rhizosphere and Root Endosphere of Wheat Plants Grown in an Andisol From Southern Chile
    Article Snippet: Root endosphere total DNA was extracted from 0.25 mL of surface sterile root homogenate using Quick-DNA™ Plant/Seed Miniprep Kit (Zymo Research Corp.), according to manufacturer instructions. .. The following PCR conditions were used: 95°C for 11 s followed by cycles of 95°C for 15s, 60°C for 30 s, and a final extension step at 75°C for 8 s and at 72°C for 10 s (Bürgmann et al., ).

    DNA Extraction:

    Article Title: A metagenomic study of DNA viruses from samples of local varieties of common bean in Kenya
    Article Snippet: .. DNA extraction and library preparation of the host plant DNA extraction was carried on 12 viral leaves with chlorosis symptomatic leaves using the Quick-DNA plant/seed miniprep kit (Zymo Cat log D6020). .. Rolling circle amplification was then carried out on each of the DNA samples using the TempliPhi™ 100 amplification kit as described by the manufacturer (GE).

    Article Title: Phylogenetic implications of nuclear rRNA IGS variation in Stipa L. (Poaceae)
    Article Snippet: Paragraph title: Plant material and DNA extraction ... Total genomic DNA was extracted from desiccated leaf tissue using ZR Plant/Seed DNA MiniPrepTM kit (Zymo Research Corp., USA) and Genomic Mini AX Plant Spin (A & A Biotechnology, Poland) following the manufacturers recommendations.

    Article Title: Endophytic Bacterial Communities Associated with Roots and Leaves of Plants Growing in Chilean Extreme Environments
    Article Snippet: .. Samples of the homogenized tissues (0.25 g) were used for DNA extraction with Quick‒DNATM Plant/seed Miniprep kits according to manufacturer instructions (Zymo Research, CA, USA). .. The quantity and purity of DNA extracts were determined by measuring absorbance at 260 nm and 280 nm by using a microplate spectrophotometer (Multiskan GO, Thermo Fisher Scientific, Inc., MA, USA).

    Article Title: Phenotypic and genetic characterisation revealed the existence of several biotypes within the Neorautanenia brachypus (Harms) C.A. wild accessions in South East Lowveld, Zimbabwe
    Article Snippet: Paragraph title: Genomic DNA isolation from N. brachypus leaves ... DNA was extracted from young N. brachypus leaves according to the ZR Plant/Seed DNA MiniPrep ™ kit protocol (Catalog No. D6020—Zymo Research Corp).

    Nucleic Acid Electrophoresis:

    Article Title: Phenotypic and genetic characterisation revealed the existence of several biotypes within the Neorautanenia brachypus (Harms) C.A. wild accessions in South East Lowveld, Zimbabwe
    Article Snippet: DNA was extracted from young N. brachypus leaves according to the ZR Plant/Seed DNA MiniPrep ™ kit protocol (Catalog No. D6020—Zymo Research Corp). .. DNA quality was determined through gel electrophoresis in EZ-vision ® (New England Bio labs) stained 1% agarose gel for sixty minutes at 100 volts in 1X Tris Borate EDTA (TBE).

    Methylation:

    Article Title: Genome-wide analysis of cytosine DNA methylation revealed salicylic acid promotes defense pathways over seedling development in pearl millet
    Article Snippet: DNA was extracted from leaves of 20 seedlings (4 lines, 5 SA doses) with four replicates using ZR plant/seed DNA miniprep (Zymo Research, Cat No. D6020) following the company protocol and an alcohol isoamyl-chloroform step was included to increase DNA yield and quality. .. The extracted DNA was subjected to a Methylation-Sensitive Amplified Polymorphism (MSAP) analysis using two isoschizomeres MspI and HpaII targeting the CCGG motifs, and EcoRI targeting GAATTC sites.

    Isolation:

    Article Title: Widespread selection and gene flow shape the genomic landscape during a radiation of monkeyflowers
    Article Snippet: .. Genomic DNA was isolated from leaf tissue using either ZR plant/seed DNA miniprep kits (Zymo Research, Irvine, CA) or GeneJet Plant Genomic DNA purification kits (Thermo Fisher, Waltham, MA). .. Illumina libraries were generated following the Allpaths-LG assembly pipeline [ ], which included a single fragment library with average 180 bp insert size and 3 mate-pair libraries (average insert sizes: 3.5–5 kb, 5–7 kb, and 7–13 kb).

    Article Title: A common phytoene synthase mutation underlies white petal varieties of the California poppy
    Article Snippet: .. Genomic DNA was isolated from commercial seeds, using the Quick-DNA Plant/Seed Miniprep Kit (Zymo Research). .. Transcriptome sequencing For transcriptome sequencing, RNAseq libraries were generated from 1 µg RNA using Illumina TruSeq RNA Library Prep Kit v2, and barcoded libraries sequenced (101 bp × 2 for flower buds, 50 bp × 1 for leaves) on an Illumina HiSeq 2000 to an average depth of 27 million reads per sample.

    Article Title: Enhanced Bacterial Wilt Resistance in Potato Through Expression of Arabidopsis EFR and Introgression of Quantitative Resistance from Solanum commersonii
    Article Snippet: .. Genomic DNA was isolated using the ZR Plant/Seed DNA MiniPrep Kit (Zymo Research) following manufacturer’s instructions. .. The PCR mix for amplification contained Taq Buffer 1× (Thermo), MgCl2 2.5 μM, dTNPs 0.5 μM, primers 0.4 μM, and Taq polymerase 1U (Invitrogen) in a final volume of 20 μL.

    Transgenic Assay:

    Article Title: Enhanced Bacterial Wilt Resistance in Potato Through Expression of Arabidopsis EFR and Introgression of Quantitative Resistance from Solanum commersonii
    Article Snippet: Paragraph title: Generation of AtEFR Transgenic Potato Lines ... Genomic DNA was isolated using the ZR Plant/Seed DNA MiniPrep Kit (Zymo Research) following manufacturer’s instructions.

    Software:

    Article Title: Comparative Analysis of Four Calypogeia Species Revealed Unexpected Change in Evolutionarily-Stable Liverwort Mitogenomes
    Article Snippet: Genome Sequencing, Assembly and Annotation Total genomic liverwort DNA was extracted using ZR Plant/Seed DNA MiniPrepTM kit (Zymo Research Corp., Irvine, CA, USA) following the manufacturer’s recommendations. .. Afterwards, the reads were mapped to the reference mitochondrial genome of Aneura pinguis using Geneious R8 software [ ].

    Real-time Polymerase Chain Reaction:

    Article Title: Putative Nitrogen-Fixing Bacteria Associated With the Rhizosphere and Root Endosphere of Wheat Plants Grown in an Andisol From Southern Chile
    Article Snippet: Total and N2 -fixing bacteria populations in rhizosphere and root endosphere samples were estimated by quantitative PCR (qPCR) using the 16S rRNA and nif H as target genes. .. Root endosphere total DNA was extracted from 0.25 mL of surface sterile root homogenate using Quick-DNA™ Plant/Seed Miniprep Kit (Zymo Research Corp.), according to manufacturer instructions.

    Agarose Gel Electrophoresis:

    Article Title: Phylogenetic implications of nuclear rRNA IGS variation in Stipa L. (Poaceae)
    Article Snippet: Total genomic DNA was extracted from desiccated leaf tissue using ZR Plant/Seed DNA MiniPrepTM kit (Zymo Research Corp., USA) and Genomic Mini AX Plant Spin (A & A Biotechnology, Poland) following the manufacturers recommendations. .. DNA quality was assessed by the 1% agarose gel electrophoresis and quantity was estimated with the use of the Qubit fluorometer system and the Quant-IT ds-DNA BR Assay kit (Invitrogen, USA).4.

    Article Title: Phenotypic and genetic characterisation revealed the existence of several biotypes within the Neorautanenia brachypus (Harms) C.A. wild accessions in South East Lowveld, Zimbabwe
    Article Snippet: DNA was extracted from young N. brachypus leaves according to the ZR Plant/Seed DNA MiniPrep ™ kit protocol (Catalog No. D6020—Zymo Research Corp). .. DNA quality was determined through gel electrophoresis in EZ-vision ® (New England Bio labs) stained 1% agarose gel for sixty minutes at 100 volts in 1X Tris Borate EDTA (TBE).

    Next-Generation Sequencing:

    Article Title: Species Composition and Trichothecene Genotype Profiling of Fusarium Field Isolates Recovered from Wheat in Poland
    Article Snippet: For next generation sequencing (NGS), DNA from 35 isolates/strains was extracted from 0.1 g of mycelium as previously described in Kulik et al. [ ]. .. Total genomic DNA was extracted with the use of the Quick-DNA Plant/Seed Miniprep Kit (Zymo Research, Irvine, CA, USA) according to the manufacturer’s protocol.

    Spectrophotometry:

    Article Title: Endophytic Bacterial Communities Associated with Roots and Leaves of Plants Growing in Chilean Extreme Environments
    Article Snippet: Samples of the homogenized tissues (0.25 g) were used for DNA extraction with Quick‒DNATM Plant/seed Miniprep kits according to manufacturer instructions (Zymo Research, CA, USA). .. The quantity and purity of DNA extracts were determined by measuring absorbance at 260 nm and 280 nm by using a microplate spectrophotometer (Multiskan GO, Thermo Fisher Scientific, Inc., MA, USA).

    DNA Purification:

    Article Title: Widespread selection and gene flow shape the genomic landscape during a radiation of monkeyflowers
    Article Snippet: .. Genomic DNA was isolated from leaf tissue using either ZR plant/seed DNA miniprep kits (Zymo Research, Irvine, CA) or GeneJet Plant Genomic DNA purification kits (Thermo Fisher, Waltham, MA). .. Illumina libraries were generated following the Allpaths-LG assembly pipeline [ ], which included a single fragment library with average 180 bp insert size and 3 mate-pair libraries (average insert sizes: 3.5–5 kb, 5–7 kb, and 7–13 kb).

    Staining:

    Article Title: Phenotypic and genetic characterisation revealed the existence of several biotypes within the Neorautanenia brachypus (Harms) C.A. wild accessions in South East Lowveld, Zimbabwe
    Article Snippet: DNA was extracted from young N. brachypus leaves according to the ZR Plant/Seed DNA MiniPrep ™ kit protocol (Catalog No. D6020—Zymo Research Corp). .. DNA quality was determined through gel electrophoresis in EZ-vision ® (New England Bio labs) stained 1% agarose gel for sixty minutes at 100 volts in 1X Tris Borate EDTA (TBE).

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  • 95
    Zymo Research zr plant seed dna miniprep kit
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    https://www.bioz.com/result/zr plant seed dna miniprep kit/product/Zymo Research
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