zymo magbead kit  (Zymo Research)


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    Name:
    Quick DNA Magbead Plus Kit
    Description:
    The Quick DNA Magbead Plus DNA Kit is the easiest method for high throughput total DNA extraction e g genomic mitochondrial viral from any biological fluid cell culture or solid tissue sample Innovative reagents and Zymo s unique system allows for a simple Bind Wash Elute procedure that is unmatched in providing ultra pure and concentrated genomic DNA 50 kb in as little as 60 minutes for 96 samples Purified DNA is ready for quantification or applications like library preparations Isolated DNA is suitable for immediate use in sensitive downstream applications including qPCR DNA seq arrays and methylation analysis
    Catalog Number:
    d4081
    Price:
    None
    Applications:
    DNA Purification
    Size:
    1 x 96 units
    Category:
    Life Science Reagents and Media
    Buy from Supplier


    Structured Review

    Zymo Research zymo magbead kit
    Quick DNA Magbead Plus Kit
    The Quick DNA Magbead Plus DNA Kit is the easiest method for high throughput total DNA extraction e g genomic mitochondrial viral from any biological fluid cell culture or solid tissue sample Innovative reagents and Zymo s unique system allows for a simple Bind Wash Elute procedure that is unmatched in providing ultra pure and concentrated genomic DNA 50 kb in as little as 60 minutes for 96 samples Purified DNA is ready for quantification or applications like library preparations Isolated DNA is suitable for immediate use in sensitive downstream applications including qPCR DNA seq arrays and methylation analysis
    https://www.bioz.com/result/zymo magbead kit/product/Zymo Research
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    zymo magbead kit - by Bioz Stars, 2020-10
    95/100 stars

    Images

    1) Product Images from "Two-phase wash to solve the ubiquitous contaminant-carryover problem in commercial nucleic-acid extraction kits"

    Article Title: Two-phase wash to solve the ubiquitous contaminant-carryover problem in commercial nucleic-acid extraction kits

    Journal: Scientific Reports

    doi: 10.1038/s41598-020-58586-3

    Evaluating TPW for compatibility with Zymo Quick-DNA/RNA MagBead extraction with ( a ) qPCR, ( b ) LAMP, and ( c ) dPCR. Extraction performed on 1 × 10 6 λ phage DNA copies with either a 10 min air dry (Manuf. protocol), no air dry, or with the air dry replaced by a TPW (+1-undecanol) step. The resulting eluent is spiked at either high dilution or low dilution into ( a ) qPCR and ( b ) LAMP or 100x dilution into ( c ) dPCR. For dPCR ( d ), the bars to the right of the solid black line show the results for an extraction protocol with a +1-undecanol wash using a high-yield protocol from a separate experiment (normalized to the no TPW control in that experiment). Bars represent single qPCR and LAMP or the merged result from a duplicate dPCR measurement. Dashed line in dPCR ( c ) indicates the average NA recovery following manufacturer protocol. We ran 9 extractions (3 magnetic-bead extractions x 3 conditions) and the eluent was shared among qPCR, LAMP, and dPCR analyses. Samples marked N.D. were not detected within either 40 cycles for qPCR or 40 min for LAMP. ( a , b ) We asked whether the manufacturer protocol replicates fell within the 95% CI of the corresponding +1-undecanol condition for the low eluent dilution case. The number of replicates that lie outside the 95% CI were indicated by the number of *s.
    Figure Legend Snippet: Evaluating TPW for compatibility with Zymo Quick-DNA/RNA MagBead extraction with ( a ) qPCR, ( b ) LAMP, and ( c ) dPCR. Extraction performed on 1 × 10 6 λ phage DNA copies with either a 10 min air dry (Manuf. protocol), no air dry, or with the air dry replaced by a TPW (+1-undecanol) step. The resulting eluent is spiked at either high dilution or low dilution into ( a ) qPCR and ( b ) LAMP or 100x dilution into ( c ) dPCR. For dPCR ( d ), the bars to the right of the solid black line show the results for an extraction protocol with a +1-undecanol wash using a high-yield protocol from a separate experiment (normalized to the no TPW control in that experiment). Bars represent single qPCR and LAMP or the merged result from a duplicate dPCR measurement. Dashed line in dPCR ( c ) indicates the average NA recovery following manufacturer protocol. We ran 9 extractions (3 magnetic-bead extractions x 3 conditions) and the eluent was shared among qPCR, LAMP, and dPCR analyses. Samples marked N.D. were not detected within either 40 cycles for qPCR or 40 min for LAMP. ( a , b ) We asked whether the manufacturer protocol replicates fell within the 95% CI of the corresponding +1-undecanol condition for the low eluent dilution case. The number of replicates that lie outside the 95% CI were indicated by the number of *s.

    Techniques Used: Real-time Polymerase Chain Reaction, Digital PCR

    2) Product Images from "Two-phase wash to solve the ubiquitous contaminant-carryover problem in commercial nucleic-acid extraction kits"

    Article Title: Two-phase wash to solve the ubiquitous contaminant-carryover problem in commercial nucleic-acid extraction kits

    Journal: Scientific Reports

    doi: 10.1038/s41598-020-58586-3

    Evaluating TPW for compatibility with Zymo Quick-DNA/RNA MagBead extraction with ( a ) qPCR, ( b ) LAMP, and ( c ) dPCR. Extraction performed on 1 × 10 6 λ phage DNA copies with either a 10 min air dry (Manuf. protocol), no air dry, or with the air dry replaced by a TPW (+1-undecanol) step. The resulting eluent is spiked at either high dilution or low dilution into ( a ) qPCR and ( b ) LAMP or 100x dilution into ( c ) dPCR. For dPCR ( d ), the bars to the right of the solid black line show the results for an extraction protocol with a +1-undecanol wash using a high-yield protocol from a separate experiment (normalized to the no TPW control in that experiment). Bars represent single qPCR and LAMP or the merged result from a duplicate dPCR measurement. Dashed line in dPCR ( c ) indicates the average NA recovery following manufacturer protocol. We ran 9 extractions (3 magnetic-bead extractions x 3 conditions) and the eluent was shared among qPCR, LAMP, and dPCR analyses. Samples marked N.D. were not detected within either 40 cycles for qPCR or 40 min for LAMP. ( a , b ) We asked whether the manufacturer protocol replicates fell within the 95% CI of the corresponding +1-undecanol condition for the low eluent dilution case. The number of replicates that lie outside the 95% CI were indicated by the number of *s.
    Figure Legend Snippet: Evaluating TPW for compatibility with Zymo Quick-DNA/RNA MagBead extraction with ( a ) qPCR, ( b ) LAMP, and ( c ) dPCR. Extraction performed on 1 × 10 6 λ phage DNA copies with either a 10 min air dry (Manuf. protocol), no air dry, or with the air dry replaced by a TPW (+1-undecanol) step. The resulting eluent is spiked at either high dilution or low dilution into ( a ) qPCR and ( b ) LAMP or 100x dilution into ( c ) dPCR. For dPCR ( d ), the bars to the right of the solid black line show the results for an extraction protocol with a +1-undecanol wash using a high-yield protocol from a separate experiment (normalized to the no TPW control in that experiment). Bars represent single qPCR and LAMP or the merged result from a duplicate dPCR measurement. Dashed line in dPCR ( c ) indicates the average NA recovery following manufacturer protocol. We ran 9 extractions (3 magnetic-bead extractions x 3 conditions) and the eluent was shared among qPCR, LAMP, and dPCR analyses. Samples marked N.D. were not detected within either 40 cycles for qPCR or 40 min for LAMP. ( a , b ) We asked whether the manufacturer protocol replicates fell within the 95% CI of the corresponding +1-undecanol condition for the low eluent dilution case. The number of replicates that lie outside the 95% CI were indicated by the number of *s.

    Techniques Used: Real-time Polymerase Chain Reaction, Digital PCR

    Related Articles

    Modification:

    Article Title: Two-phase wash to solve the ubiquitous contaminant-carryover problem in commercial nucleic-acid extraction kits
    Article Snippet: .. In the modified protocol for the Zymo MagBead kit, we waited at least one additional minute and perform a second aspiration after each aspiration step in the manufacturer’s protocol. .. qPCR mix qPCR reactions contained 1X Bio-Rad SsoFast Supermix (1725201, Bio-Rad), PCR primers (IDT) at 0.5 µM each, and were supplemented with nuclease-free water up to 10 µL.

    DNA Extraction:

    Article Title: Optimal protocols for sequence-based characterization of the human vaginal microbiome
    Article Snippet: .. DNA extraction DNA extraction was performed with the Quick-DNA Magbead Plus kit (D4082 - Zymo Research, Irvine, CA, USA), according to manufacturer instructions with few modifications. .. Prior to extraction the samples were bead-beaten for 1 minute at 1600 RPM using ZR Bashing Bead Lysis matrix (S6012 - Zymo Research, Irvine, CA, USA).

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  • 95
    Zymo Research zymo magbead kit
    Evaluating TPW for compatibility with <t>Zymo</t> Quick-DNA/RNA <t>MagBead</t> extraction with ( a ) qPCR, ( b ) LAMP, and ( c ) dPCR. Extraction performed on 1 × 10 6 λ phage DNA copies with either a 10 min air dry (Manuf. protocol), no air dry, or with the air dry replaced by a TPW (+1-undecanol) step. The resulting eluent is spiked at either high dilution or low dilution into ( a ) qPCR and ( b ) LAMP or 100x dilution into ( c ) dPCR. For dPCR ( d ), the bars to the right of the solid black line show the results for an extraction protocol with a +1-undecanol wash using a high-yield protocol from a separate experiment (normalized to the no TPW control in that experiment). Bars represent single qPCR and LAMP or the merged result from a duplicate dPCR measurement. Dashed line in dPCR ( c ) indicates the average NA recovery following manufacturer protocol. We ran 9 extractions (3 magnetic-bead extractions x 3 conditions) and the eluent was shared among qPCR, LAMP, and dPCR analyses. Samples marked N.D. were not detected within either 40 cycles for qPCR or 40 min for LAMP. ( a , b ) We asked whether the manufacturer protocol replicates fell within the 95% CI of the corresponding +1-undecanol condition for the low eluent dilution case. The number of replicates that lie outside the 95% CI were indicated by the number of *s.
    Zymo Magbead Kit, supplied by Zymo Research, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/zymo magbead kit/product/Zymo Research
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    zymo magbead kit - by Bioz Stars, 2020-10
    95/100 stars
      Buy from Supplier

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    Evaluating TPW for compatibility with Zymo Quick-DNA/RNA MagBead extraction with ( a ) qPCR, ( b ) LAMP, and ( c ) dPCR. Extraction performed on 1 × 10 6 λ phage DNA copies with either a 10 min air dry (Manuf. protocol), no air dry, or with the air dry replaced by a TPW (+1-undecanol) step. The resulting eluent is spiked at either high dilution or low dilution into ( a ) qPCR and ( b ) LAMP or 100x dilution into ( c ) dPCR. For dPCR ( d ), the bars to the right of the solid black line show the results for an extraction protocol with a +1-undecanol wash using a high-yield protocol from a separate experiment (normalized to the no TPW control in that experiment). Bars represent single qPCR and LAMP or the merged result from a duplicate dPCR measurement. Dashed line in dPCR ( c ) indicates the average NA recovery following manufacturer protocol. We ran 9 extractions (3 magnetic-bead extractions x 3 conditions) and the eluent was shared among qPCR, LAMP, and dPCR analyses. Samples marked N.D. were not detected within either 40 cycles for qPCR or 40 min for LAMP. ( a , b ) We asked whether the manufacturer protocol replicates fell within the 95% CI of the corresponding +1-undecanol condition for the low eluent dilution case. The number of replicates that lie outside the 95% CI were indicated by the number of *s.

    Journal: Scientific Reports

    Article Title: Two-phase wash to solve the ubiquitous contaminant-carryover problem in commercial nucleic-acid extraction kits

    doi: 10.1038/s41598-020-58586-3

    Figure Lengend Snippet: Evaluating TPW for compatibility with Zymo Quick-DNA/RNA MagBead extraction with ( a ) qPCR, ( b ) LAMP, and ( c ) dPCR. Extraction performed on 1 × 10 6 λ phage DNA copies with either a 10 min air dry (Manuf. protocol), no air dry, or with the air dry replaced by a TPW (+1-undecanol) step. The resulting eluent is spiked at either high dilution or low dilution into ( a ) qPCR and ( b ) LAMP or 100x dilution into ( c ) dPCR. For dPCR ( d ), the bars to the right of the solid black line show the results for an extraction protocol with a +1-undecanol wash using a high-yield protocol from a separate experiment (normalized to the no TPW control in that experiment). Bars represent single qPCR and LAMP or the merged result from a duplicate dPCR measurement. Dashed line in dPCR ( c ) indicates the average NA recovery following manufacturer protocol. We ran 9 extractions (3 magnetic-bead extractions x 3 conditions) and the eluent was shared among qPCR, LAMP, and dPCR analyses. Samples marked N.D. were not detected within either 40 cycles for qPCR or 40 min for LAMP. ( a , b ) We asked whether the manufacturer protocol replicates fell within the 95% CI of the corresponding +1-undecanol condition for the low eluent dilution case. The number of replicates that lie outside the 95% CI were indicated by the number of *s.

    Article Snippet: For the Zymo MagBead kit, 200 µL sample was mixed with 200 µL Zymo 2x DNA/RNA Shield, 4 µL Proteinase K, and 800 µL Zymo Viral DNA/RNA Buffer.

    Techniques: Real-time Polymerase Chain Reaction, Digital PCR