quick cfdna serum  (Zymo Research)


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    Name:
    Quick cfDNA Serum Plasma Kit
    Description:
    Quick cfDNA Serum Plasma Kit provides a simple and reliable method for the rapid preparation of high quality circulating cell free DNA from serum plasma amniotic fluid cerebrospinal fluid CSF and saliva A combination of chemical and enzymatic methods are used to efficiently recover total DNA including cell free apoptotic necrotic mitochondrial and viral DNA linearly from up to 10 ml of sample Reference Figure A Zymo Spin technology allows for ultra pure DNA to be eluted in as little as 35 μl water The resulting DNA is suitable for all subsequent analyses and molecular manipulations such as qPCR Next Generation sequencing and DNA methylation analyses Zymo s serum and plasma cell free DNA extraction technology will empower your discovery of circulating DNA biomarkers
    Catalog Number:
    d4076
    Price:
    None
    Applications:
    DNA Purification from Cell-Free, Serum, Plasma, Liquid Biopsies
    Size:
    50 units
    Category:
    Life Science Reagents and Media
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    Structured Review

    Zymo Research quick cfdna serum
    Quick cfDNA Serum Plasma Kit
    Quick cfDNA Serum Plasma Kit provides a simple and reliable method for the rapid preparation of high quality circulating cell free DNA from serum plasma amniotic fluid cerebrospinal fluid CSF and saliva A combination of chemical and enzymatic methods are used to efficiently recover total DNA including cell free apoptotic necrotic mitochondrial and viral DNA linearly from up to 10 ml of sample Reference Figure A Zymo Spin technology allows for ultra pure DNA to be eluted in as little as 35 μl water The resulting DNA is suitable for all subsequent analyses and molecular manipulations such as qPCR Next Generation sequencing and DNA methylation analyses Zymo s serum and plasma cell free DNA extraction technology will empower your discovery of circulating DNA biomarkers
    https://www.bioz.com/result/quick cfdna serum/product/Zymo Research
    Average 95 stars, based on 329 article reviews
    Price from $9.99 to $1999.99
    quick cfdna serum - by Bioz Stars, 2020-02
    95/100 stars

    Images

    1) Product Images from "T Oligo-Primed Polymerase Chain Reaction (TOP-PCR): A Robust Method for the Amplification of Minute DNA Fragments in Body Fluids"

    Article Title: T Oligo-Primed Polymerase Chain Reaction (TOP-PCR): A Robust Method for the Amplification of Minute DNA Fragments in Body Fluids

    Journal: Scientific Reports

    doi: 10.1038/srep40767

    Comparison of TOP-PCR to Illumina’s PCR method using serial dilutions of plasma cfDNA sample. Serial dilutions (5 ng–0.01 pg) of a plasma cfDNA sample isolated from a healthy female ( BBC ) was prepared and the cfDNA is amplified using either Illumina’s PCR or TOP-PCR. TOP panel: profiles generated by Illumina’s PCR method. Lower panel: profiles generated by TOP-PCR. Notice that the RFU values are no longer accurate because of figure overlay. PCR cycle numbers: 30 for 5–0.5 ng; 40 for 0.05 ng–0.01 pg. Size markers: 1 bp and 6000 bp peaks. Size of two HAs added to each DNA fragment: ~22 bp.
    Figure Legend Snippet: Comparison of TOP-PCR to Illumina’s PCR method using serial dilutions of plasma cfDNA sample. Serial dilutions (5 ng–0.01 pg) of a plasma cfDNA sample isolated from a healthy female ( BBC ) was prepared and the cfDNA is amplified using either Illumina’s PCR or TOP-PCR. TOP panel: profiles generated by Illumina’s PCR method. Lower panel: profiles generated by TOP-PCR. Notice that the RFU values are no longer accurate because of figure overlay. PCR cycle numbers: 30 for 5–0.5 ng; 40 for 0.05 ng–0.01 pg. Size markers: 1 bp and 6000 bp peaks. Size of two HAs added to each DNA fragment: ~22 bp.

    Techniques Used: Polymerase Chain Reaction, Isolation, Amplification, Generated

    Test of TOP-PCR reproducibility and cfDNA consistency. Two blood samples were separately collected from a healthy male ( YFH ) on June 30, 2015 and October 28, 2015. Plasmas were prepared right after the blood collections and stored at −80 °C. Samples of cfDNA were extracted right before TOP-PCR reactions conducted on October 28, 2015. Blue, plasma stock prepared on June 30, 2015; red and black, plasma stock prepared on October 1, 2015. Size markers: 35 bp and 6000 bp peaks. Size of two HAs added to each DNA fragment: ~22 bp.
    Figure Legend Snippet: Test of TOP-PCR reproducibility and cfDNA consistency. Two blood samples were separately collected from a healthy male ( YFH ) on June 30, 2015 and October 28, 2015. Plasmas were prepared right after the blood collections and stored at −80 °C. Samples of cfDNA were extracted right before TOP-PCR reactions conducted on October 28, 2015. Blue, plasma stock prepared on June 30, 2015; red and black, plasma stock prepared on October 1, 2015. Size markers: 35 bp and 6000 bp peaks. Size of two HAs added to each DNA fragment: ~22 bp.

    Techniques Used: Polymerase Chain Reaction

    TOP-PCR amplification of saliva and urine cfDNA. ( a ) Size profile comparison between the original and TOP-PCR amplified normal saliva DNA samples. The saliva cfDNA from a healthy male individual (YFH) was amplified by TOP-PCR and displayed in parallel with the original. (Black, 5 ng of original; blue, 1 ng of TOP-PCR product). ( b ) Comparison between the original and TOP-PCR amplified normal urine cfDNA samples. Urine sample from the same healthy male ( a ) was tested. (Black, original urine DNA; blue, 40-cycle TOP-PCR amplification of 0.1 ng of the original. 5 ng each was displayed by Fragment Analyzer. Size markers: 35 bp and 6000 bp peaks. Size of two HAs added to each DNA fragment: ~22 bp.
    Figure Legend Snippet: TOP-PCR amplification of saliva and urine cfDNA. ( a ) Size profile comparison between the original and TOP-PCR amplified normal saliva DNA samples. The saliva cfDNA from a healthy male individual (YFH) was amplified by TOP-PCR and displayed in parallel with the original. (Black, 5 ng of original; blue, 1 ng of TOP-PCR product). ( b ) Comparison between the original and TOP-PCR amplified normal urine cfDNA samples. Urine sample from the same healthy male ( a ) was tested. (Black, original urine DNA; blue, 40-cycle TOP-PCR amplification of 0.1 ng of the original. 5 ng each was displayed by Fragment Analyzer. Size markers: 35 bp and 6000 bp peaks. Size of two HAs added to each DNA fragment: ~22 bp.

    Techniques Used: Polymerase Chain Reaction, Amplification

    Comparison of TOP-PCR with Illumina’s PCR method using low amount of DNA as the input. ( a ) One micro-liter of original ovarian cancer plasma cfDNA sample with unknown concentration. ( b ) Same DNA sample but after 50 cycles of amplification by TOP-PCR. ( c ) Same DNA sample but after 50 cycles of amplification using Illumina’s protocol. Size markers: 35 bp and 6000 bp peaks. Size of two HAs added to each DNA fragment: ~22 bp.
    Figure Legend Snippet: Comparison of TOP-PCR with Illumina’s PCR method using low amount of DNA as the input. ( a ) One micro-liter of original ovarian cancer plasma cfDNA sample with unknown concentration. ( b ) Same DNA sample but after 50 cycles of amplification by TOP-PCR. ( c ) Same DNA sample but after 50 cycles of amplification using Illumina’s protocol. Size markers: 35 bp and 6000 bp peaks. Size of two HAs added to each DNA fragment: ~22 bp.

    Techniques Used: Polymerase Chain Reaction, Concentration Assay, Amplification

    Comparison of TOP-PCT with Illumina’s PCR method using 20 ng of DNA. ( a ) One nano-gram original plasma cfDNA sample isolated from a healthy female (BBC). ( b ) Same DNA sample but after 20 cycles of amplification using TOP-PCR. ( c ) Same DNA sample but after 20 cycles of amplification using Illumina’s protocol. Size markers: 35 bp and 6000 bp peaks. Size of two HAs added to each DNA fragment: ~22 bp.
    Figure Legend Snippet: Comparison of TOP-PCT with Illumina’s PCR method using 20 ng of DNA. ( a ) One nano-gram original plasma cfDNA sample isolated from a healthy female (BBC). ( b ) Same DNA sample but after 20 cycles of amplification using TOP-PCR. ( c ) Same DNA sample but after 20 cycles of amplification using Illumina’s protocol. Size markers: 35 bp and 6000 bp peaks. Size of two HAs added to each DNA fragment: ~22 bp.

    Techniques Used: Polymerase Chain Reaction, Isolation, Amplification

    Related Articles

    DNA Extraction:

    Article Title: Circulating tumor DNA as a liquid biopsy target for detection of pancreatic cancer
    Article Snippet: .. Recently, there are an increasing number of new products for cfDNA processing including blood collection tubes [e.g ., Cell-Free DNA BCT® (Streck) and Cell-Free DNA Collection Tube (Roche)] and cfDNA extraction kits [e.g ., Quick-cfDNA™ Serum & Plasma Kit (Zymo Research), Maxwell® RSC ccfDNA Plasma Kit (Promega), and MagMAX™ Cell-Free DNA Isolation Kit (Thermo Fisher Scientific)]. .. For sequencing of cfDNA, new library preparation kits optimized for small amounts of fragmented DNA, such as Accel-NGS® DNA Library Kits (Swift Biosciences) and ThruPLEX® Plasma-seq Kit (Rubicon Genomics), have also been available.

    Article Title: Successful early fetal sex determination using cell-free fetal DNA isolated from maternal capillary blood: A pilot study
    Article Snippet: .. DNA extraction cffDNA was extracted from the whole volume of collected capillary blood (up to 2 ml) using the Quick-cfDNA™ Serum & Plasma Kit (Zymo Research, USA) according to the manufacturer’s instructions. ..

    Article Title: Detection of Islet β-Cell Death in Vivo by Multiplex PCR Analysis of Differentially Methylated DNA
    Article Snippet: Paragraph title: DNA extraction and bisulfite treatment ... DNA was extracted from 20 μL of serum/plasma samples using the ZR Serum DNA kit (Zymo Research Corp, Orange, California).

    Article Title: Potential Diagnosis of Vitreoretinal Lymphoma by Detection of MYD88 Mutation in Aqueous Humor With Ultrasensitive Droplet Digital Polymerase Chain Reaction
    Article Snippet: .. Cell-free DNA isolation of the supernatant was performed with the Quick-cfDNA Serum and Plasma Kit (Zymo Research). ..

    Article Title: T Oligo-Primed Polymerase Chain Reaction (TOP-PCR): A Robust Method for the Amplification of Minute DNA Fragments in Body Fluids
    Article Snippet: Paragraph title: Cell-free DNA isolation from blood plasma and saliva ... Plasma DNA was isolated by Quick-cfDNA Serum and Plasma Kit (Zymo Research, D4076) following the manufacturer’s instructions.

    Article Title: Preanalytical blood sample workup for cell‐free DNA analysis using Droplet Digital PCR for future molecular cancer diagnostics
    Article Snippet: .. DNA isolation methods For comparison of DNA isolation methods, the following isolation kits were used: Jena PME free‐circulating DNA extraction kit (Analytik Jena, Germany), QIAamp Circulating NA Kit (Qiagen, Hilden, Germany), QIAsymphony Circulating NA kit (Qiagen, Hilden, Germany), MagNAPure LC Total Nucleic Acid Isolation Large Volume kit (Roche Life Science, Basel, Switzerland), and Zymo Quick cfDNA serum & plasma kit (Zymo Research, Irvine, CA, USA). ..

    Diagnostic Assay:

    Article Title: Potential Diagnosis of Vitreoretinal Lymphoma by Detection of MYD88 Mutation in Aqueous Humor With Ultrasensitive Droplet Digital Polymerase Chain Reaction
    Article Snippet: We had the opportunity to test MYD88 L265P ddPCR in 3 available samples of diluted vitreous aspiration fluid that had been obtained during diagnostic vitrectomy; these revealed cytomorphological detection of tumor cells in the diluted vitreous humor. .. Cell-free DNA isolation of the supernatant was performed with the Quick-cfDNA Serum and Plasma Kit (Zymo Research).

    Centrifugation:

    Article Title: Epigenetic markers in circulating cell‐free DNA as prognostic markers for survival of castration‐resistant prostate cancer patients, et al. Epigenetic markers in circulating cell‐free DNA as prognostic markers for survival of castration‐resistant prostate cancer patients
    Article Snippet: The EDTA tubes were centrifuged at 1.300 g for 10 min after which plasma was extracted and stored within two hours at −80°C. cfDNA was extracted from 1.5 mL of plasma using the Quick‐cfDNA Serum & Plasma kit (Zymo Research D4076). .. First, a centrifugation step of 16.000 g for 10 min was performed to ensure efficient depletion of residual cells, cell debris, and genomic DNA.

    Article Title: Epigenetic markers in circulating cell‐free DNA as prognostic markers for survival of castration‐resistant prostate cancer patients, et al. Epigenetic markers in circulating cell‐free DNA as prognostic markers for survival of castration‐resistant prostate cancer patients
    Article Snippet: The EDTA tubes were centrifuged at 1.300g for 10 min after which plasma was extracted and stored within two hours at −80°C. cfDNA was extracted from 1.5 mL of plasma using the Quick‐cfDNA Serum & Plasma kit (Zymo Research D4076). .. First, a centrifugation step of 16.000g for 10 min was performed to ensure efficient depletion of residual cells, cell debris, and genomic DNA.

    Variant Assay:

    Article Title: Technical and Regulatory Considerations for Taking Liquid Biopsy to the Clinic: Validation of the JAX PlasmaMonitorTM Assay
    Article Snippet: As our NGS panel was developed, technical and analytical factors were evaluated to develop a test with high sensitivity and specificity, keeping in mind the LOD for low ( < 1%) variant allelic frequency (VAF). .. We evaluated four commercial cfDNA extraction kits across 35 samples; QIAamp circulating nucleic acid kit (Qiagen), NucleoSpin Plasma XS (Macherey-Nagel), ZR Serum DNA kit (ZYMO Research), and the NEXTPrep-Mag cfDNA isolation kit (Bioo Scientific) ( ).

    Isolation:

    Article Title: Potential Diagnosis of Vitreoretinal Lymphoma by Detection of MYD88 Mutation in Aqueous Humor With Ultrasensitive Droplet Digital Polymerase Chain Reaction
    Article Snippet: Cell-free DNA isolation of the supernatant was performed with the Quick-cfDNA Serum and Plasma Kit (Zymo Research). .. The isolation of DNA from cell pellets of the diluted and undiluted VF samples was achieved by direct lysis in 30 μL of 50mM TRIS/hydrochloric acid buffer and 10 μL of proteinase K (10 mg/mL), after which the mixture is heated for 1 hour at 56°C, cooked for 10 minutes, and centrifuged for 2 minutes at 8000 rpm.

    Article Title: Analysis of microbial sequences in plasma cell-free DNA for early-onset breast cancer patients and healthy females
    Article Snippet: .. All plasma cfDNA samples were isolated from blood using Quick-cfDNA Serum and Plasma Kit (Zymo Research, D4076) following manufacturer’s instructions. ..

    Article Title: T Oligo-Primed Polymerase Chain Reaction (TOP-PCR): A Robust Method for the Amplification of Minute DNA Fragments in Body Fluids
    Article Snippet: .. Plasma DNA was isolated by Quick-cfDNA Serum and Plasma Kit (Zymo Research, D4076) following the manufacturer’s instructions. .. Briefly, plasma samples ( < 5 ml each) were incubated with proteinase K in digestion buffer for 30 min in a 55 °C water bath, then mixed with DNA binding buffer and loaded into the column to allow DNA to bind to the column membrane.

    Article Title: Preanalytical blood sample workup for cell‐free DNA analysis using Droplet Digital PCR for future molecular cancer diagnostics
    Article Snippet: .. DNA isolation methods For comparison of DNA isolation methods, the following isolation kits were used: Jena PME free‐circulating DNA extraction kit (Analytik Jena, Germany), QIAamp Circulating NA Kit (Qiagen, Hilden, Germany), QIAsymphony Circulating NA kit (Qiagen, Hilden, Germany), MagNAPure LC Total Nucleic Acid Isolation Large Volume kit (Roche Life Science, Basel, Switzerland), and Zymo Quick cfDNA serum & plasma kit (Zymo Research, Irvine, CA, USA). ..

    Article Title: Technical and Regulatory Considerations for Taking Liquid Biopsy to the Clinic: Validation of the JAX PlasmaMonitorTM Assay
    Article Snippet: .. We evaluated four commercial cfDNA extraction kits across 35 samples; QIAamp circulating nucleic acid kit (Qiagen), NucleoSpin Plasma XS (Macherey-Nagel), ZR Serum DNA kit (ZYMO Research), and the NEXTPrep-Mag cfDNA isolation kit (Bioo Scientific) ( ). .. Based on the yield, scalability, turnaround time for processing, and cost effectiveness, we opted for the NEXTPrep-Mag cfDNA isolation kit.

    Next-Generation Sequencing:

    Article Title: Technical and Regulatory Considerations for Taking Liquid Biopsy to the Clinic: Validation of the JAX PlasmaMonitorTM Assay
    Article Snippet: As our NGS panel was developed, technical and analytical factors were evaluated to develop a test with high sensitivity and specificity, keeping in mind the LOD for low ( < 1%) variant allelic frequency (VAF). .. We evaluated four commercial cfDNA extraction kits across 35 samples; QIAamp circulating nucleic acid kit (Qiagen), NucleoSpin Plasma XS (Macherey-Nagel), ZR Serum DNA kit (ZYMO Research), and the NEXTPrep-Mag cfDNA isolation kit (Bioo Scientific) ( ).

    DNA Methylation Assay:

    Article Title: Detection of Islet β-Cell Death in Vivo by Multiplex PCR Analysis of Differentially Methylated DNA
    Article Snippet: DNA was extracted from 20 μL of serum/plasma samples using the ZR Serum DNA kit (Zymo Research Corp, Orange, California). .. All DNA samples underwent bisulfite treatment using the EZ DNA Methylation kit (Zymo Research).

    Sequencing:

    Article Title: Circulating tumor DNA as a liquid biopsy target for detection of pancreatic cancer
    Article Snippet: Recently, there are an increasing number of new products for cfDNA processing including blood collection tubes [e.g ., Cell-Free DNA BCT® (Streck) and Cell-Free DNA Collection Tube (Roche)] and cfDNA extraction kits [e.g ., Quick-cfDNA™ Serum & Plasma Kit (Zymo Research), Maxwell® RSC ccfDNA Plasma Kit (Promega), and MagMAX™ Cell-Free DNA Isolation Kit (Thermo Fisher Scientific)]. .. For sequencing of cfDNA, new library preparation kits optimized for small amounts of fragmented DNA, such as Accel-NGS® DNA Library Kits (Swift Biosciences) and ThruPLEX® Plasma-seq Kit (Rubicon Genomics), have also been available.

    Article Title: Technical and Regulatory Considerations for Taking Liquid Biopsy to the Clinic: Validation of the JAX PlasmaMonitorTM Assay
    Article Snippet: We evaluated four commercial cfDNA extraction kits across 35 samples; QIAamp circulating nucleic acid kit (Qiagen), NucleoSpin Plasma XS (Macherey-Nagel), ZR Serum DNA kit (ZYMO Research), and the NEXTPrep-Mag cfDNA isolation kit (Bioo Scientific) ( ). .. Current cfDNA extraction methods yield modest quantities of nucleic acid content, emphasizing the importance of sensitive and accurate quantification and qualification of cfDNA in the molecular sequencing process.

    Incubation:

    Article Title: T Oligo-Primed Polymerase Chain Reaction (TOP-PCR): A Robust Method for the Amplification of Minute DNA Fragments in Body Fluids
    Article Snippet: Plasma DNA was isolated by Quick-cfDNA Serum and Plasma Kit (Zymo Research, D4076) following the manufacturer’s instructions. .. Briefly, plasma samples ( < 5 ml each) were incubated with proteinase K in digestion buffer for 30 min in a 55 °C water bath, then mixed with DNA binding buffer and loaded into the column to allow DNA to bind to the column membrane.

    Selection:

    Article Title: Technical and Regulatory Considerations for Taking Liquid Biopsy to the Clinic: Validation of the JAX PlasmaMonitorTM Assay
    Article Snippet: Optimization of pre-analytical parameters such as plasma separation and selection of an isolation method that ensures extraction of a sufficient amount of high-quality DNA is critical. .. We evaluated four commercial cfDNA extraction kits across 35 samples; QIAamp circulating nucleic acid kit (Qiagen), NucleoSpin Plasma XS (Macherey-Nagel), ZR Serum DNA kit (ZYMO Research), and the NEXTPrep-Mag cfDNA isolation kit (Bioo Scientific) ( ).

    Modification:

    Article Title: Technical and Regulatory Considerations for Taking Liquid Biopsy to the Clinic: Validation of the JAX PlasmaMonitorTM Assay
    Article Snippet: We evaluated four commercial cfDNA extraction kits across 35 samples; QIAamp circulating nucleic acid kit (Qiagen), NucleoSpin Plasma XS (Macherey-Nagel), ZR Serum DNA kit (ZYMO Research), and the NEXTPrep-Mag cfDNA isolation kit (Bioo Scientific) ( ). .. Although we realize that a bioanalyzer analysis to assess fragment size and quality is widely accepted to be the norm, we evaluated and validated the use of a modified Human Genomic DNA (HGD) QC Assay for quantification and qualification of the extracted cfDNA to enable effective downstream processing, taking into consideration DNA integrity index (DIN).

    Lysis:

    Article Title: Potential Diagnosis of Vitreoretinal Lymphoma by Detection of MYD88 Mutation in Aqueous Humor With Ultrasensitive Droplet Digital Polymerase Chain Reaction
    Article Snippet: Cell-free DNA isolation of the supernatant was performed with the Quick-cfDNA Serum and Plasma Kit (Zymo Research). .. The isolation of DNA from cell pellets of the diluted and undiluted VF samples was achieved by direct lysis in 30 μL of 50mM TRIS/hydrochloric acid buffer and 10 μL of proteinase K (10 mg/mL), after which the mixture is heated for 1 hour at 56°C, cooked for 10 minutes, and centrifuged for 2 minutes at 8000 rpm.

    Sampling:

    Article Title: Circulating tumor DNA as a liquid biopsy target for detection of pancreatic cancer
    Article Snippet: Prospective follow-up and sequential blood sampling of individuals at high risk of pancreatic cancer (e.g ., those with a family history of pancreatic cancer or chronic pancreatitis) might thus be essential. .. Recently, there are an increasing number of new products for cfDNA processing including blood collection tubes [e.g ., Cell-Free DNA BCT® (Streck) and Cell-Free DNA Collection Tube (Roche)] and cfDNA extraction kits [e.g ., Quick-cfDNA™ Serum & Plasma Kit (Zymo Research), Maxwell® RSC ccfDNA Plasma Kit (Promega), and MagMAX™ Cell-Free DNA Isolation Kit (Thermo Fisher Scientific)].

    Article Title: Technical and Regulatory Considerations for Taking Liquid Biopsy to the Clinic: Validation of the JAX PlasmaMonitorTM Assay
    Article Snippet: It has been shown that these pre-analytical factors of blood sampling and processing can strongly affect DNA yield as well as downstream analysis. .. We evaluated four commercial cfDNA extraction kits across 35 samples; QIAamp circulating nucleic acid kit (Qiagen), NucleoSpin Plasma XS (Macherey-Nagel), ZR Serum DNA kit (ZYMO Research), and the NEXTPrep-Mag cfDNA isolation kit (Bioo Scientific) ( ).

    Binding Assay:

    Article Title: T Oligo-Primed Polymerase Chain Reaction (TOP-PCR): A Robust Method for the Amplification of Minute DNA Fragments in Body Fluids
    Article Snippet: Plasma DNA was isolated by Quick-cfDNA Serum and Plasma Kit (Zymo Research, D4076) following the manufacturer’s instructions. .. Briefly, plasma samples ( < 5 ml each) were incubated with proteinase K in digestion buffer for 30 min in a 55 °C water bath, then mixed with DNA binding buffer and loaded into the column to allow DNA to bind to the column membrane.

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    Zymo Research quick cfdna serum
    Comparison of TOP-PCR to Illumina’s PCR method using serial dilutions of plasma <t>cfDNA</t> sample. Serial dilutions (5 ng–0.01 pg) of a plasma cfDNA sample isolated from a healthy female ( BBC ) was prepared and the cfDNA is amplified using either Illumina’s PCR or TOP-PCR. TOP panel: profiles generated by Illumina’s PCR method. Lower panel: profiles generated by TOP-PCR. Notice that the RFU values are no longer accurate because of figure overlay. PCR cycle numbers: 30 for 5–0.5 ng; 40 for 0.05 ng–0.01 pg. Size markers: 1 bp and 6000 bp peaks. Size of two HAs added to each <t>DNA</t> fragment: ~22 bp.
    Quick Cfdna Serum, supplied by Zymo Research, used in various techniques. Bioz Stars score: 95/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quick cfdna serum/product/Zymo Research
    Average 95 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    quick cfdna serum - by Bioz Stars, 2020-02
    95/100 stars
      Buy from Supplier

    95
    Zymo Research quick dna microprep
    Comparison of TOP-PCR to Illumina’s PCR method using serial dilutions of plasma <t>cfDNA</t> sample. Serial dilutions (5 ng–0.01 pg) of a plasma cfDNA sample isolated from a healthy female ( BBC ) was prepared and the cfDNA is amplified using either Illumina’s PCR or TOP-PCR. TOP panel: profiles generated by Illumina’s PCR method. Lower panel: profiles generated by TOP-PCR. Notice that the RFU values are no longer accurate because of figure overlay. PCR cycle numbers: 30 for 5–0.5 ng; 40 for 0.05 ng–0.01 pg. Size markers: 1 bp and 6000 bp peaks. Size of two HAs added to each <t>DNA</t> fragment: ~22 bp.
    Quick Dna Microprep, supplied by Zymo Research, used in various techniques. Bioz Stars score: 95/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quick dna microprep/product/Zymo Research
    Average 95 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    quick dna microprep - by Bioz Stars, 2020-02
    95/100 stars
      Buy from Supplier

    95
    Zymo Research quick gdna midiprep kit
    Comparison of TOP-PCR to Illumina’s PCR method using serial dilutions of plasma <t>cfDNA</t> sample. Serial dilutions (5 ng–0.01 pg) of a plasma cfDNA sample isolated from a healthy female ( BBC ) was prepared and the cfDNA is amplified using either Illumina’s PCR or TOP-PCR. TOP panel: profiles generated by Illumina’s PCR method. Lower panel: profiles generated by TOP-PCR. Notice that the RFU values are no longer accurate because of figure overlay. PCR cycle numbers: 30 for 5–0.5 ng; 40 for 0.05 ng–0.01 pg. Size markers: 1 bp and 6000 bp peaks. Size of two HAs added to each <t>DNA</t> fragment: ~22 bp.
    Quick Gdna Midiprep Kit, supplied by Zymo Research, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quick gdna midiprep kit/product/Zymo Research
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    quick gdna midiprep kit - by Bioz Stars, 2020-02
    95/100 stars
      Buy from Supplier

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    Comparison of TOP-PCR to Illumina’s PCR method using serial dilutions of plasma cfDNA sample. Serial dilutions (5 ng–0.01 pg) of a plasma cfDNA sample isolated from a healthy female ( BBC ) was prepared and the cfDNA is amplified using either Illumina’s PCR or TOP-PCR. TOP panel: profiles generated by Illumina’s PCR method. Lower panel: profiles generated by TOP-PCR. Notice that the RFU values are no longer accurate because of figure overlay. PCR cycle numbers: 30 for 5–0.5 ng; 40 for 0.05 ng–0.01 pg. Size markers: 1 bp and 6000 bp peaks. Size of two HAs added to each DNA fragment: ~22 bp.

    Journal: Scientific Reports

    Article Title: T Oligo-Primed Polymerase Chain Reaction (TOP-PCR): A Robust Method for the Amplification of Minute DNA Fragments in Body Fluids

    doi: 10.1038/srep40767

    Figure Lengend Snippet: Comparison of TOP-PCR to Illumina’s PCR method using serial dilutions of plasma cfDNA sample. Serial dilutions (5 ng–0.01 pg) of a plasma cfDNA sample isolated from a healthy female ( BBC ) was prepared and the cfDNA is amplified using either Illumina’s PCR or TOP-PCR. TOP panel: profiles generated by Illumina’s PCR method. Lower panel: profiles generated by TOP-PCR. Notice that the RFU values are no longer accurate because of figure overlay. PCR cycle numbers: 30 for 5–0.5 ng; 40 for 0.05 ng–0.01 pg. Size markers: 1 bp and 6000 bp peaks. Size of two HAs added to each DNA fragment: ~22 bp.

    Article Snippet: Plasma DNA was isolated by Quick-cfDNA Serum and Plasma Kit (Zymo Research, D4076) following the manufacturer’s instructions.

    Techniques: Polymerase Chain Reaction, Isolation, Amplification, Generated

    Test of TOP-PCR reproducibility and cfDNA consistency. Two blood samples were separately collected from a healthy male ( YFH ) on June 30, 2015 and October 28, 2015. Plasmas were prepared right after the blood collections and stored at −80 °C. Samples of cfDNA were extracted right before TOP-PCR reactions conducted on October 28, 2015. Blue, plasma stock prepared on June 30, 2015; red and black, plasma stock prepared on October 1, 2015. Size markers: 35 bp and 6000 bp peaks. Size of two HAs added to each DNA fragment: ~22 bp.

    Journal: Scientific Reports

    Article Title: T Oligo-Primed Polymerase Chain Reaction (TOP-PCR): A Robust Method for the Amplification of Minute DNA Fragments in Body Fluids

    doi: 10.1038/srep40767

    Figure Lengend Snippet: Test of TOP-PCR reproducibility and cfDNA consistency. Two blood samples were separately collected from a healthy male ( YFH ) on June 30, 2015 and October 28, 2015. Plasmas were prepared right after the blood collections and stored at −80 °C. Samples of cfDNA were extracted right before TOP-PCR reactions conducted on October 28, 2015. Blue, plasma stock prepared on June 30, 2015; red and black, plasma stock prepared on October 1, 2015. Size markers: 35 bp and 6000 bp peaks. Size of two HAs added to each DNA fragment: ~22 bp.

    Article Snippet: Plasma DNA was isolated by Quick-cfDNA Serum and Plasma Kit (Zymo Research, D4076) following the manufacturer’s instructions.

    Techniques: Polymerase Chain Reaction

    TOP-PCR amplification of saliva and urine cfDNA. ( a ) Size profile comparison between the original and TOP-PCR amplified normal saliva DNA samples. The saliva cfDNA from a healthy male individual (YFH) was amplified by TOP-PCR and displayed in parallel with the original. (Black, 5 ng of original; blue, 1 ng of TOP-PCR product). ( b ) Comparison between the original and TOP-PCR amplified normal urine cfDNA samples. Urine sample from the same healthy male ( a ) was tested. (Black, original urine DNA; blue, 40-cycle TOP-PCR amplification of 0.1 ng of the original. 5 ng each was displayed by Fragment Analyzer. Size markers: 35 bp and 6000 bp peaks. Size of two HAs added to each DNA fragment: ~22 bp.

    Journal: Scientific Reports

    Article Title: T Oligo-Primed Polymerase Chain Reaction (TOP-PCR): A Robust Method for the Amplification of Minute DNA Fragments in Body Fluids

    doi: 10.1038/srep40767

    Figure Lengend Snippet: TOP-PCR amplification of saliva and urine cfDNA. ( a ) Size profile comparison between the original and TOP-PCR amplified normal saliva DNA samples. The saliva cfDNA from a healthy male individual (YFH) was amplified by TOP-PCR and displayed in parallel with the original. (Black, 5 ng of original; blue, 1 ng of TOP-PCR product). ( b ) Comparison between the original and TOP-PCR amplified normal urine cfDNA samples. Urine sample from the same healthy male ( a ) was tested. (Black, original urine DNA; blue, 40-cycle TOP-PCR amplification of 0.1 ng of the original. 5 ng each was displayed by Fragment Analyzer. Size markers: 35 bp and 6000 bp peaks. Size of two HAs added to each DNA fragment: ~22 bp.

    Article Snippet: Plasma DNA was isolated by Quick-cfDNA Serum and Plasma Kit (Zymo Research, D4076) following the manufacturer’s instructions.

    Techniques: Polymerase Chain Reaction, Amplification

    Comparison of TOP-PCR with Illumina’s PCR method using low amount of DNA as the input. ( a ) One micro-liter of original ovarian cancer plasma cfDNA sample with unknown concentration. ( b ) Same DNA sample but after 50 cycles of amplification by TOP-PCR. ( c ) Same DNA sample but after 50 cycles of amplification using Illumina’s protocol. Size markers: 35 bp and 6000 bp peaks. Size of two HAs added to each DNA fragment: ~22 bp.

    Journal: Scientific Reports

    Article Title: T Oligo-Primed Polymerase Chain Reaction (TOP-PCR): A Robust Method for the Amplification of Minute DNA Fragments in Body Fluids

    doi: 10.1038/srep40767

    Figure Lengend Snippet: Comparison of TOP-PCR with Illumina’s PCR method using low amount of DNA as the input. ( a ) One micro-liter of original ovarian cancer plasma cfDNA sample with unknown concentration. ( b ) Same DNA sample but after 50 cycles of amplification by TOP-PCR. ( c ) Same DNA sample but after 50 cycles of amplification using Illumina’s protocol. Size markers: 35 bp and 6000 bp peaks. Size of two HAs added to each DNA fragment: ~22 bp.

    Article Snippet: Plasma DNA was isolated by Quick-cfDNA Serum and Plasma Kit (Zymo Research, D4076) following the manufacturer’s instructions.

    Techniques: Polymerase Chain Reaction, Concentration Assay, Amplification

    Comparison of TOP-PCT with Illumina’s PCR method using 20 ng of DNA. ( a ) One nano-gram original plasma cfDNA sample isolated from a healthy female (BBC). ( b ) Same DNA sample but after 20 cycles of amplification using TOP-PCR. ( c ) Same DNA sample but after 20 cycles of amplification using Illumina’s protocol. Size markers: 35 bp and 6000 bp peaks. Size of two HAs added to each DNA fragment: ~22 bp.

    Journal: Scientific Reports

    Article Title: T Oligo-Primed Polymerase Chain Reaction (TOP-PCR): A Robust Method for the Amplification of Minute DNA Fragments in Body Fluids

    doi: 10.1038/srep40767

    Figure Lengend Snippet: Comparison of TOP-PCT with Illumina’s PCR method using 20 ng of DNA. ( a ) One nano-gram original plasma cfDNA sample isolated from a healthy female (BBC). ( b ) Same DNA sample but after 20 cycles of amplification using TOP-PCR. ( c ) Same DNA sample but after 20 cycles of amplification using Illumina’s protocol. Size markers: 35 bp and 6000 bp peaks. Size of two HAs added to each DNA fragment: ~22 bp.

    Article Snippet: Plasma DNA was isolated by Quick-cfDNA Serum and Plasma Kit (Zymo Research, D4076) following the manufacturer’s instructions.

    Techniques: Polymerase Chain Reaction, Isolation, Amplification