zr fecal dna isolation kit  (Zymo Research)


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    Name:
    Quick DNA Fecal Soil Microbe Microprep
    Description:
    The Quick DNA Fecal Soil Microbe Microprep Kit is designed for the simple and rapid isolation of inhibitor free PCR quality host cell and microbial DNA from a variety of sample sources including humans birds rats mice cattle etc The procedure is easy and can be completed in minutes fecal samples are rapidly and efficiently lysed by bead beating with our state of the art ultra high density BashingBeads Zymo Spin column technology is then used to isolate the DNA which is subsequently filtered to remove humic acids polyphenols that can inhibit PCR Eluted DNA is ideal for downstream molecular based applications including PCR arrays genotyping methylation detection etc
    Catalog Number:
    d6012
    Product Aliases:
    ZR Fecal DNA MicroPrep
    Price:
    None
    Applications:
    DNA Purification
    Size:
    50 units
    Category:
    Life Science Reagents and Media
    Buy from Supplier


    Structured Review

    Zymo Research zr fecal dna isolation kit
    Quick DNA Fecal Soil Microbe Microprep
    The Quick DNA Fecal Soil Microbe Microprep Kit is designed for the simple and rapid isolation of inhibitor free PCR quality host cell and microbial DNA from a variety of sample sources including humans birds rats mice cattle etc The procedure is easy and can be completed in minutes fecal samples are rapidly and efficiently lysed by bead beating with our state of the art ultra high density BashingBeads Zymo Spin column technology is then used to isolate the DNA which is subsequently filtered to remove humic acids polyphenols that can inhibit PCR Eluted DNA is ideal for downstream molecular based applications including PCR arrays genotyping methylation detection etc
    https://www.bioz.com/result/zr fecal dna isolation kit/product/Zymo Research
    Average 99 stars, based on 549 article reviews
    Price from $9.99 to $1999.99
    zr fecal dna isolation kit - by Bioz Stars, 2020-10
    99/100 stars

    Images

    1) Product Images from "OPTIMIZING THE ANALYSIS OF HUMAN INTESTINAL MICROBIOTA WITH PHYLOGENETIC MICROARRAY"

    Article Title: OPTIMIZING THE ANALYSIS OF HUMAN INTESTINAL MICROBIOTA WITH PHYLOGENETIC MICROARRAY

    Journal: FEMS microbiology ecology

    doi: 10.1111/j.1574-6941.2010.01009.x

    Correlation among measured microarray signals obtained in interrogation of total RNA and genomic DNA
    Figure Legend Snippet: Correlation among measured microarray signals obtained in interrogation of total RNA and genomic DNA

    Techniques Used: Microarray

    2) Product Images from "An efficient and cost-effective method for DNA extraction from athalassohaline soil using a newly formulated cell extraction buffer"

    Article Title: An efficient and cost-effective method for DNA extraction from athalassohaline soil using a newly formulated cell extraction buffer

    Journal: 3 Biotech

    doi: 10.1007/s13205-016-0383-0

    A Epifluorescence microscopic images of acridine orange stained slides, ( a ) intact soil sample and ( b ) soil sample after cell extraction. B Acridine orange staining-based microbial cell count by epifluoresence microscopy before and after cell extraction. C Electrophoresed 1 % gel showing amplified 16S rRNA gene, M molecular weight marker, lane 1 : amplified 16S rRNA gene from environmental DNA extracted by newly developed method, lane 2–7 : amplification of 16S rRNA gene from DNA extracted by published methods and kits, (it can be observed that DNA was unable to amplify by these methods). D Electrophoresed 1 % agarose gel showing, lane M : SuperMix DNA ladder (0.5 kb to 33 kb), lane 1 : environmental DNA, lane 2 : mixture of environmental DNA extracted from newly developed method and λ DNA digested with Hind III, lane 3 : λ DNA digested with Hind III and. E Polyacrylamide gel (9 %) showing: lane M molecular weight marker 100 bp, lane 2 : λ DNA digested using Hind III, lane 3 : completely digested environmental DNA extracted with newly developed method with Hae III (10 h), and lane 4 : partially digested environmental DNA extracted with newly developed method with Hae III (1 h). F Electrophoresed 1 % agarose gel showing DNA marker and extracted environmental DNA by various methods. M denotes molecular weight marker, lane 1 : showing environmental DNA extracted by newly developed method, lane 2 : high concentration of lysozyme lysis method (Gabor et al. 2003 ), lane 3 : hot detergent lysis method (Desai and Madamwar 2006 ), lane 4 : bead beating lane (Miller et al. 1999 ) 5 : NucleoSpin Soil Extract II, lane 6 : Soil gDNA isolation kit (XcelGen), lane 7 : ZR Soil Microbe DNA MiniPrep. G UV–visible absorbance spectra of environmental DNA extracted by described mentioned methods and kits
    Figure Legend Snippet: A Epifluorescence microscopic images of acridine orange stained slides, ( a ) intact soil sample and ( b ) soil sample after cell extraction. B Acridine orange staining-based microbial cell count by epifluoresence microscopy before and after cell extraction. C Electrophoresed 1 % gel showing amplified 16S rRNA gene, M molecular weight marker, lane 1 : amplified 16S rRNA gene from environmental DNA extracted by newly developed method, lane 2–7 : amplification of 16S rRNA gene from DNA extracted by published methods and kits, (it can be observed that DNA was unable to amplify by these methods). D Electrophoresed 1 % agarose gel showing, lane M : SuperMix DNA ladder (0.5 kb to 33 kb), lane 1 : environmental DNA, lane 2 : mixture of environmental DNA extracted from newly developed method and λ DNA digested with Hind III, lane 3 : λ DNA digested with Hind III and. E Polyacrylamide gel (9 %) showing: lane M molecular weight marker 100 bp, lane 2 : λ DNA digested using Hind III, lane 3 : completely digested environmental DNA extracted with newly developed method with Hae III (10 h), and lane 4 : partially digested environmental DNA extracted with newly developed method with Hae III (1 h). F Electrophoresed 1 % agarose gel showing DNA marker and extracted environmental DNA by various methods. M denotes molecular weight marker, lane 1 : showing environmental DNA extracted by newly developed method, lane 2 : high concentration of lysozyme lysis method (Gabor et al. 2003 ), lane 3 : hot detergent lysis method (Desai and Madamwar 2006 ), lane 4 : bead beating lane (Miller et al. 1999 ) 5 : NucleoSpin Soil Extract II, lane 6 : Soil gDNA isolation kit (XcelGen), lane 7 : ZR Soil Microbe DNA MiniPrep. G UV–visible absorbance spectra of environmental DNA extracted by described mentioned methods and kits

    Techniques Used: Staining, Cell Counting, Microscopy, Amplification, Molecular Weight, Marker, Agarose Gel Electrophoresis, Concentration Assay, Lysis, Isolation

    3) Product Images from "SELECTIVE INDUCTION OF HOMEOSTATIC TH17 CELLS IN THE MURINE INTESTINE BY CHOLERA TOXIN INTERACTING WITH THE MICROBIOTA"

    Article Title: SELECTIVE INDUCTION OF HOMEOSTATIC TH17 CELLS IN THE MURINE INTESTINE BY CHOLERA TOXIN INTERACTING WITH THE MICROBIOTA

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    doi: 10.4049/jimmunol.1700171

    Intestinal microbiota contribute to the immune differences between IgA −/− and WT mice (A–D) Ileal and cecal contents were collected from 5 adult IgA −/− and WT mice of parent strains, respectively. Bacterial genomic DNA was extracted and used for 16s rDNA microbiome sequencing. (A) Pie charts show phyla as percent of total bacteria. (B) Principle coordinate analysis shows ileal and cecal bacterial composition of IgA −/− and WT mice of the parent strains. Each dot represents an individual mouse. The ratios of Firmicutes over Bacteroidetes in the ileum and cecum of parent strain IgA −/− and WT mice are shown in (C). (D) SFB prevalence in the ileum and cecum of parent strain IgA −/− and WT mice. (E) Breeding strategy for intestinal microbiota equalization between IgA −/− and WT mice. Female IgA −/− mice were crossed with male WT mice (which were removed from breeding cages before pups were born), to obtain F1 heterozygous mice. F1 mice were crossed to obtain homozygous IgA −/− and WT littermates in the F2 generation. Number in parentheses indicates expected frequency of homozygotes in the F2 generation. (F) Littermate IgA −/− and WT mice were immunized intragastrically with 10μg CT and 1mg Ovalbumin (OVA) in 500μl 0.2M sodium bicarbonate on days 0, 7, and 14, IL-17A expression in CD4 + siLPLs of immunized mice and naïve control mice is shown (n=4 per group). (G) CTB- and OVA- specific IgG responses (n=4 per group) and (H) LCN-2 level in the sera of littermate IgA −/− and WT mice (n=6 per group) were measured with ELISA. All of the results are representative of at least two independent experiments.
    Figure Legend Snippet: Intestinal microbiota contribute to the immune differences between IgA −/− and WT mice (A–D) Ileal and cecal contents were collected from 5 adult IgA −/− and WT mice of parent strains, respectively. Bacterial genomic DNA was extracted and used for 16s rDNA microbiome sequencing. (A) Pie charts show phyla as percent of total bacteria. (B) Principle coordinate analysis shows ileal and cecal bacterial composition of IgA −/− and WT mice of the parent strains. Each dot represents an individual mouse. The ratios of Firmicutes over Bacteroidetes in the ileum and cecum of parent strain IgA −/− and WT mice are shown in (C). (D) SFB prevalence in the ileum and cecum of parent strain IgA −/− and WT mice. (E) Breeding strategy for intestinal microbiota equalization between IgA −/− and WT mice. Female IgA −/− mice were crossed with male WT mice (which were removed from breeding cages before pups were born), to obtain F1 heterozygous mice. F1 mice were crossed to obtain homozygous IgA −/− and WT littermates in the F2 generation. Number in parentheses indicates expected frequency of homozygotes in the F2 generation. (F) Littermate IgA −/− and WT mice were immunized intragastrically with 10μg CT and 1mg Ovalbumin (OVA) in 500μl 0.2M sodium bicarbonate on days 0, 7, and 14, IL-17A expression in CD4 + siLPLs of immunized mice and naïve control mice is shown (n=4 per group). (G) CTB- and OVA- specific IgG responses (n=4 per group) and (H) LCN-2 level in the sera of littermate IgA −/− and WT mice (n=6 per group) were measured with ELISA. All of the results are representative of at least two independent experiments.

    Techniques Used: Mouse Assay, Sequencing, Expressing, CtB Assay, Enzyme-linked Immunosorbent Assay

    4) Product Images from "Reconstructing a herbivore’s diet using a novel rbcL DNA mini-barcode for plants"

    Article Title: Reconstructing a herbivore’s diet using a novel rbcL DNA mini-barcode for plants

    Journal: AoB Plants

    doi: 10.1093/aobpla/plx015

    Flow chart of procedures for collecting, extracting and quantifying DNA used to identify deer forage species and deer identification for 12 samples. (1) Samples were collected within a 1 km 2 at Smithsonian’s Conservation Biology in Front Royal, VA in October 2014. (2) The DNA extraction was conducted using Zymo Fecal DNA Mini-prep Kit. (3a) Known microsatellite markers were used verify each of the 12 samples were from separate individuals. (3b) In parallel to the PCR based mini-barcode approach, WGS data was generated and analysed using five whole chloroplast sequences in CLC Bio. (3c) PCR was conducted using primers designed for mini-Barcode rbc l region. (4a) Sequencing of amplicons was performed on an Illumina MiSeq V2. (4b) Voucher DNA Barcode samples from plant species collected in region were obtained from GenBank. (5) Data quality control was performed in CLC Bio Genomics Workbench (ver. 7.4) while bioinformatics analysis, where sequences were assigned taxonomic identity, were performed using Qiime. (6) Primary statistics contrasting animal diet included PERMANOVA and ANOSIM which utilized OTU tables from Qiime analysis.
    Figure Legend Snippet: Flow chart of procedures for collecting, extracting and quantifying DNA used to identify deer forage species and deer identification for 12 samples. (1) Samples were collected within a 1 km 2 at Smithsonian’s Conservation Biology in Front Royal, VA in October 2014. (2) The DNA extraction was conducted using Zymo Fecal DNA Mini-prep Kit. (3a) Known microsatellite markers were used verify each of the 12 samples were from separate individuals. (3b) In parallel to the PCR based mini-barcode approach, WGS data was generated and analysed using five whole chloroplast sequences in CLC Bio. (3c) PCR was conducted using primers designed for mini-Barcode rbc l region. (4a) Sequencing of amplicons was performed on an Illumina MiSeq V2. (4b) Voucher DNA Barcode samples from plant species collected in region were obtained from GenBank. (5) Data quality control was performed in CLC Bio Genomics Workbench (ver. 7.4) while bioinformatics analysis, where sequences were assigned taxonomic identity, were performed using Qiime. (6) Primary statistics contrasting animal diet included PERMANOVA and ANOSIM which utilized OTU tables from Qiime analysis.

    Techniques Used: Flow Cytometry, DNA Extraction, Polymerase Chain Reaction, Generated, Sequencing

    Related Articles

    DNA Extraction:

    Article Title: SELECTIVE INDUCTION OF HOMEOSTATIC TH17 CELLS IN THE MURINE INTESTINE BY CHOLERA TOXIN INTERACTING WITH THE MICROBIOTA
    Article Snippet: .. Reagents and materials were purchased from the following sources: anti–mouse CD4 (RM4-5), CD25 (PC61) antibodies were purchased from BD Biosciences; anti-mouse IL-17A (TC11-18H10.1), IFN-γ (XMG1.2), CD90.1 (OX7) antibodies were purchased from BioLegend; anti-mouse Foxp3 (FJK-16s) and IL-22 (1H8PWSR) antibodies as well as Foxp3 staining buffer set were purchased from eBioscience; anti-mouse CD90.1 (19E.2) and anti-human CD2 (OKT11) were purchased from the hybridoma lab of Epitope Recognition and Immunoreagent Core at UAB; live/dead staining kit was purchased from Invitrogen; mouse lamina propria dissociation kit and regulatory T cell isolation kit were purchased from Miltenyi Biotec; mouse LCN-2 ELISA kit was purchased from R & D Systems; fecal DNA extraction kit was purchased from Zymo Research. .. Small and large intestines were removed, sliced, and washed with ice-cold PBS to remove fecal content.

    Article Title: Human Catestatin Alters Gut Microbiota Composition in Mice
    Article Snippet: .. Fecal DNA extraction was performed using a ZR fecal DNA extraction kit (Zymo Research Corp., Orange, CA). .. Both DNA extraction kits had a bead-beating step to mechanically lyse microbial cells.

    Article Title: OPTIMIZING THE ANALYSIS OF HUMAN INTESTINAL MICROBIOTA WITH PHYLOGENETIC MICROARRAY
    Article Snippet: .. Total genomic DNA (gDNA) was isolated from fecal samples using the ZR Fecal DNA Isolation kit (Zymo Research Corporation) according to manufacturer’s protocol. .. For RNA isolation, an aliquot of stool/preservative mixture was thawed, centrifuged for 5 minutes, and the supernatant was removed.

    Article Title: Draft Genome Sequences of a Novel Lineage of Armatimonadetes Recovered from Japanese Hot Springs
    Article Snippet: .. Cells were disrupted by attaching sample tubes to the blade of a cordless reciprocating saw and operating for 1 min. DNA was purified with a Zymo soil/fecal DNA extraction kit (Zymo Research, Irvine, CA) and quantified with a Qubit 3.0 fluorimeter (Life Technologies, Inc., Carlsbad, CA). .. DNA was submitted to SeqMatic LLC (Fremont, CA) for library preparation and sequencing by Illumina HiSeq.

    Cell Isolation:

    Article Title: SELECTIVE INDUCTION OF HOMEOSTATIC TH17 CELLS IN THE MURINE INTESTINE BY CHOLERA TOXIN INTERACTING WITH THE MICROBIOTA
    Article Snippet: .. Reagents and materials were purchased from the following sources: anti–mouse CD4 (RM4-5), CD25 (PC61) antibodies were purchased from BD Biosciences; anti-mouse IL-17A (TC11-18H10.1), IFN-γ (XMG1.2), CD90.1 (OX7) antibodies were purchased from BioLegend; anti-mouse Foxp3 (FJK-16s) and IL-22 (1H8PWSR) antibodies as well as Foxp3 staining buffer set were purchased from eBioscience; anti-mouse CD90.1 (19E.2) and anti-human CD2 (OKT11) were purchased from the hybridoma lab of Epitope Recognition and Immunoreagent Core at UAB; live/dead staining kit was purchased from Invitrogen; mouse lamina propria dissociation kit and regulatory T cell isolation kit were purchased from Miltenyi Biotec; mouse LCN-2 ELISA kit was purchased from R & D Systems; fecal DNA extraction kit was purchased from Zymo Research. .. Small and large intestines were removed, sliced, and washed with ice-cold PBS to remove fecal content.

    Amplification:

    Article Title: Akkermansia muciniphila is permissive to arthritis in the K/BxN mouse model of arthritis
    Article Snippet: .. Briefly, fecal DNA was purified with the Zymo Research kit (Irvine, CA), and the V4 16S rDNA region was amplified and sequenced on the MiSeq apparatus (Illumina, San Diego, CA). ..

    Article Title: Detection and treatment strategy for Tritrichomonas muris in the common laboratory mouse
    Article Snippet: .. T. muris specific PCR amplification and visualization Fresh fecal pellets were collected from mice and fecal DNA extracted using the ZR Fecal DNA MiniPrep™ kit (Zymo Research Corp., CA, U.S.A.). .. T. muris forward and reverse primers were purchased through Integrated DNA Technologies (IDTDNA.com).

    Article Title: Reconstructing a herbivore’s diet using a novel rbcL DNA mini-barcode for plants
    Article Snippet: .. Fecal pellets had total genomic DNA extracted using the Zymo fecal DNA kit, with the resulting DNA PCR amplified using a rbc L-mini-barcode marker set, first described in this paper. .. The rbc L mini-barcode marker exhibited a high rate of species level identification, and could resolve all sequences from plants found at the site to at least genus, if not species ( ).

    Isolation:

    Article Title: OPTIMIZING THE ANALYSIS OF HUMAN INTESTINAL MICROBIOTA WITH PHYLOGENETIC MICROARRAY
    Article Snippet: .. Total genomic DNA (gDNA) was isolated from fecal samples using the ZR Fecal DNA Isolation kit (Zymo Research Corporation) according to manufacturer’s protocol. .. For RNA isolation, an aliquot of stool/preservative mixture was thawed, centrifuged for 5 minutes, and the supernatant was removed.

    Purification:

    Article Title: Akkermansia muciniphila is permissive to arthritis in the K/BxN mouse model of arthritis
    Article Snippet: .. Briefly, fecal DNA was purified with the Zymo Research kit (Irvine, CA), and the V4 16S rDNA region was amplified and sequenced on the MiSeq apparatus (Illumina, San Diego, CA). ..

    Article Title: Draft Genome Sequences of a Novel Lineage of Armatimonadetes Recovered from Japanese Hot Springs
    Article Snippet: .. Cells were disrupted by attaching sample tubes to the blade of a cordless reciprocating saw and operating for 1 min. DNA was purified with a Zymo soil/fecal DNA extraction kit (Zymo Research, Irvine, CA) and quantified with a Qubit 3.0 fluorimeter (Life Technologies, Inc., Carlsbad, CA). .. DNA was submitted to SeqMatic LLC (Fremont, CA) for library preparation and sequencing by Illumina HiSeq.

    Enzyme-linked Immunosorbent Assay:

    Article Title: SELECTIVE INDUCTION OF HOMEOSTATIC TH17 CELLS IN THE MURINE INTESTINE BY CHOLERA TOXIN INTERACTING WITH THE MICROBIOTA
    Article Snippet: .. Reagents and materials were purchased from the following sources: anti–mouse CD4 (RM4-5), CD25 (PC61) antibodies were purchased from BD Biosciences; anti-mouse IL-17A (TC11-18H10.1), IFN-γ (XMG1.2), CD90.1 (OX7) antibodies were purchased from BioLegend; anti-mouse Foxp3 (FJK-16s) and IL-22 (1H8PWSR) antibodies as well as Foxp3 staining buffer set were purchased from eBioscience; anti-mouse CD90.1 (19E.2) and anti-human CD2 (OKT11) were purchased from the hybridoma lab of Epitope Recognition and Immunoreagent Core at UAB; live/dead staining kit was purchased from Invitrogen; mouse lamina propria dissociation kit and regulatory T cell isolation kit were purchased from Miltenyi Biotec; mouse LCN-2 ELISA kit was purchased from R & D Systems; fecal DNA extraction kit was purchased from Zymo Research. .. Small and large intestines were removed, sliced, and washed with ice-cold PBS to remove fecal content.

    Polymerase Chain Reaction:

    Article Title: Detection and treatment strategy for Tritrichomonas muris in the common laboratory mouse
    Article Snippet: .. T. muris specific PCR amplification and visualization Fresh fecal pellets were collected from mice and fecal DNA extracted using the ZR Fecal DNA MiniPrep™ kit (Zymo Research Corp., CA, U.S.A.). .. T. muris forward and reverse primers were purchased through Integrated DNA Technologies (IDTDNA.com).

    Article Title: Reconstructing a herbivore’s diet using a novel rbcL DNA mini-barcode for plants
    Article Snippet: .. Fecal pellets had total genomic DNA extracted using the Zymo fecal DNA kit, with the resulting DNA PCR amplified using a rbc L-mini-barcode marker set, first described in this paper. .. The rbc L mini-barcode marker exhibited a high rate of species level identification, and could resolve all sequences from plants found at the site to at least genus, if not species ( ).

    Marker:

    Article Title: Reconstructing a herbivore’s diet using a novel rbcL DNA mini-barcode for plants
    Article Snippet: .. Fecal pellets had total genomic DNA extracted using the Zymo fecal DNA kit, with the resulting DNA PCR amplified using a rbc L-mini-barcode marker set, first described in this paper. .. The rbc L mini-barcode marker exhibited a high rate of species level identification, and could resolve all sequences from plants found at the site to at least genus, if not species ( ).

    Staining:

    Article Title: SELECTIVE INDUCTION OF HOMEOSTATIC TH17 CELLS IN THE MURINE INTESTINE BY CHOLERA TOXIN INTERACTING WITH THE MICROBIOTA
    Article Snippet: .. Reagents and materials were purchased from the following sources: anti–mouse CD4 (RM4-5), CD25 (PC61) antibodies were purchased from BD Biosciences; anti-mouse IL-17A (TC11-18H10.1), IFN-γ (XMG1.2), CD90.1 (OX7) antibodies were purchased from BioLegend; anti-mouse Foxp3 (FJK-16s) and IL-22 (1H8PWSR) antibodies as well as Foxp3 staining buffer set were purchased from eBioscience; anti-mouse CD90.1 (19E.2) and anti-human CD2 (OKT11) were purchased from the hybridoma lab of Epitope Recognition and Immunoreagent Core at UAB; live/dead staining kit was purchased from Invitrogen; mouse lamina propria dissociation kit and regulatory T cell isolation kit were purchased from Miltenyi Biotec; mouse LCN-2 ELISA kit was purchased from R & D Systems; fecal DNA extraction kit was purchased from Zymo Research. .. Small and large intestines were removed, sliced, and washed with ice-cold PBS to remove fecal content.

    Mouse Assay:

    Article Title: Detection and treatment strategy for Tritrichomonas muris in the common laboratory mouse
    Article Snippet: .. T. muris specific PCR amplification and visualization Fresh fecal pellets were collected from mice and fecal DNA extracted using the ZR Fecal DNA MiniPrep™ kit (Zymo Research Corp., CA, U.S.A.). .. T. muris forward and reverse primers were purchased through Integrated DNA Technologies (IDTDNA.com).

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    Zymo Research zr fecal dna isolation kit
    Correlation among measured microarray signals obtained in interrogation of total RNA and genomic <t>DNA</t>
    Zr Fecal Dna Isolation Kit, supplied by Zymo Research, used in various techniques. Bioz Stars score: 99/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/zr fecal dna isolation kit/product/Zymo Research
    Average 99 stars, based on 36 article reviews
    Price from $9.99 to $1999.99
    zr fecal dna isolation kit - by Bioz Stars, 2020-10
    99/100 stars
      Buy from Supplier

    99
    Zymo Research zr soil microbe dna miniprep
    A Epifluorescence microscopic images of acridine orange stained slides, ( a ) intact soil sample and ( b ) soil sample after cell extraction. B Acridine orange staining-based microbial cell count by epifluoresence microscopy before and after cell extraction. C Electrophoresed 1 % gel showing amplified 16S rRNA gene, M molecular weight marker, lane 1 : amplified 16S rRNA gene from environmental <t>DNA</t> extracted by newly developed method, lane 2–7 : amplification of 16S rRNA gene from DNA extracted by published methods and kits, (it can be observed that DNA was unable to amplify by these methods). D Electrophoresed 1 % agarose gel showing, lane M : SuperMix DNA ladder (0.5 kb to 33 kb), lane 1 : environmental DNA, lane 2 : mixture of environmental DNA extracted from newly developed method and λ DNA digested with Hind III, lane 3 : λ DNA digested with Hind III and. E Polyacrylamide gel (9 %) showing: lane M molecular weight marker 100 bp, lane 2 : λ DNA digested using Hind III, lane 3 : completely digested environmental DNA extracted with newly developed method with Hae III (10 h), and lane 4 : partially digested environmental DNA extracted with newly developed method with Hae III (1 h). F Electrophoresed 1 % agarose gel showing DNA marker and extracted environmental DNA by various methods. M denotes molecular weight marker, lane 1 : showing environmental DNA extracted by newly developed method, lane 2 : high concentration of lysozyme lysis method (Gabor et al. 2003 ), lane 3 : hot detergent lysis method (Desai and Madamwar 2006 ), lane 4 : bead beating lane (Miller et al. 1999 ) 5 : NucleoSpin Soil Extract II, lane 6 : Soil gDNA isolation kit (XcelGen), lane 7 : ZR Soil Microbe DNA <t>MiniPrep.</t> G UV–visible absorbance spectra of environmental DNA extracted by described mentioned methods and kits
    Zr Soil Microbe Dna Miniprep, supplied by Zymo Research, used in various techniques. Bioz Stars score: 99/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/zr soil microbe dna miniprep/product/Zymo Research
    Average 99 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    zr soil microbe dna miniprep - by Bioz Stars, 2020-10
    99/100 stars
      Buy from Supplier

    Image Search Results


    Correlation among measured microarray signals obtained in interrogation of total RNA and genomic DNA

    Journal: FEMS microbiology ecology

    Article Title: OPTIMIZING THE ANALYSIS OF HUMAN INTESTINAL MICROBIOTA WITH PHYLOGENETIC MICROARRAY

    doi: 10.1111/j.1574-6941.2010.01009.x

    Figure Lengend Snippet: Correlation among measured microarray signals obtained in interrogation of total RNA and genomic DNA

    Article Snippet: Total genomic DNA (gDNA) was isolated from fecal samples using the ZR Fecal DNA Isolation kit (Zymo Research Corporation) according to manufacturer’s protocol.

    Techniques: Microarray

    Intestinal microbiota contribute to the immune differences between IgA −/− and WT mice (A–D) Ileal and cecal contents were collected from 5 adult IgA −/− and WT mice of parent strains, respectively. Bacterial genomic DNA was extracted and used for 16s rDNA microbiome sequencing. (A) Pie charts show phyla as percent of total bacteria. (B) Principle coordinate analysis shows ileal and cecal bacterial composition of IgA −/− and WT mice of the parent strains. Each dot represents an individual mouse. The ratios of Firmicutes over Bacteroidetes in the ileum and cecum of parent strain IgA −/− and WT mice are shown in (C). (D) SFB prevalence in the ileum and cecum of parent strain IgA −/− and WT mice. (E) Breeding strategy for intestinal microbiota equalization between IgA −/− and WT mice. Female IgA −/− mice were crossed with male WT mice (which were removed from breeding cages before pups were born), to obtain F1 heterozygous mice. F1 mice were crossed to obtain homozygous IgA −/− and WT littermates in the F2 generation. Number in parentheses indicates expected frequency of homozygotes in the F2 generation. (F) Littermate IgA −/− and WT mice were immunized intragastrically with 10μg CT and 1mg Ovalbumin (OVA) in 500μl 0.2M sodium bicarbonate on days 0, 7, and 14, IL-17A expression in CD4 + siLPLs of immunized mice and naïve control mice is shown (n=4 per group). (G) CTB- and OVA- specific IgG responses (n=4 per group) and (H) LCN-2 level in the sera of littermate IgA −/− and WT mice (n=6 per group) were measured with ELISA. All of the results are representative of at least two independent experiments.

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: SELECTIVE INDUCTION OF HOMEOSTATIC TH17 CELLS IN THE MURINE INTESTINE BY CHOLERA TOXIN INTERACTING WITH THE MICROBIOTA

    doi: 10.4049/jimmunol.1700171

    Figure Lengend Snippet: Intestinal microbiota contribute to the immune differences between IgA −/− and WT mice (A–D) Ileal and cecal contents were collected from 5 adult IgA −/− and WT mice of parent strains, respectively. Bacterial genomic DNA was extracted and used for 16s rDNA microbiome sequencing. (A) Pie charts show phyla as percent of total bacteria. (B) Principle coordinate analysis shows ileal and cecal bacterial composition of IgA −/− and WT mice of the parent strains. Each dot represents an individual mouse. The ratios of Firmicutes over Bacteroidetes in the ileum and cecum of parent strain IgA −/− and WT mice are shown in (C). (D) SFB prevalence in the ileum and cecum of parent strain IgA −/− and WT mice. (E) Breeding strategy for intestinal microbiota equalization between IgA −/− and WT mice. Female IgA −/− mice were crossed with male WT mice (which were removed from breeding cages before pups were born), to obtain F1 heterozygous mice. F1 mice were crossed to obtain homozygous IgA −/− and WT littermates in the F2 generation. Number in parentheses indicates expected frequency of homozygotes in the F2 generation. (F) Littermate IgA −/− and WT mice were immunized intragastrically with 10μg CT and 1mg Ovalbumin (OVA) in 500μl 0.2M sodium bicarbonate on days 0, 7, and 14, IL-17A expression in CD4 + siLPLs of immunized mice and naïve control mice is shown (n=4 per group). (G) CTB- and OVA- specific IgG responses (n=4 per group) and (H) LCN-2 level in the sera of littermate IgA −/− and WT mice (n=6 per group) were measured with ELISA. All of the results are representative of at least two independent experiments.

    Article Snippet: Reagents and materials were purchased from the following sources: anti–mouse CD4 (RM4-5), CD25 (PC61) antibodies were purchased from BD Biosciences; anti-mouse IL-17A (TC11-18H10.1), IFN-γ (XMG1.2), CD90.1 (OX7) antibodies were purchased from BioLegend; anti-mouse Foxp3 (FJK-16s) and IL-22 (1H8PWSR) antibodies as well as Foxp3 staining buffer set were purchased from eBioscience; anti-mouse CD90.1 (19E.2) and anti-human CD2 (OKT11) were purchased from the hybridoma lab of Epitope Recognition and Immunoreagent Core at UAB; live/dead staining kit was purchased from Invitrogen; mouse lamina propria dissociation kit and regulatory T cell isolation kit were purchased from Miltenyi Biotec; mouse LCN-2 ELISA kit was purchased from R & D Systems; fecal DNA extraction kit was purchased from Zymo Research.

    Techniques: Mouse Assay, Sequencing, Expressing, CtB Assay, Enzyme-linked Immunosorbent Assay

    Flow chart of procedures for collecting, extracting and quantifying DNA used to identify deer forage species and deer identification for 12 samples. (1) Samples were collected within a 1 km 2 at Smithsonian’s Conservation Biology in Front Royal, VA in October 2014. (2) The DNA extraction was conducted using Zymo Fecal DNA Mini-prep Kit. (3a) Known microsatellite markers were used verify each of the 12 samples were from separate individuals. (3b) In parallel to the PCR based mini-barcode approach, WGS data was generated and analysed using five whole chloroplast sequences in CLC Bio. (3c) PCR was conducted using primers designed for mini-Barcode rbc l region. (4a) Sequencing of amplicons was performed on an Illumina MiSeq V2. (4b) Voucher DNA Barcode samples from plant species collected in region were obtained from GenBank. (5) Data quality control was performed in CLC Bio Genomics Workbench (ver. 7.4) while bioinformatics analysis, where sequences were assigned taxonomic identity, were performed using Qiime. (6) Primary statistics contrasting animal diet included PERMANOVA and ANOSIM which utilized OTU tables from Qiime analysis.

    Journal: AoB Plants

    Article Title: Reconstructing a herbivore’s diet using a novel rbcL DNA mini-barcode for plants

    doi: 10.1093/aobpla/plx015

    Figure Lengend Snippet: Flow chart of procedures for collecting, extracting and quantifying DNA used to identify deer forage species and deer identification for 12 samples. (1) Samples were collected within a 1 km 2 at Smithsonian’s Conservation Biology in Front Royal, VA in October 2014. (2) The DNA extraction was conducted using Zymo Fecal DNA Mini-prep Kit. (3a) Known microsatellite markers were used verify each of the 12 samples were from separate individuals. (3b) In parallel to the PCR based mini-barcode approach, WGS data was generated and analysed using five whole chloroplast sequences in CLC Bio. (3c) PCR was conducted using primers designed for mini-Barcode rbc l region. (4a) Sequencing of amplicons was performed on an Illumina MiSeq V2. (4b) Voucher DNA Barcode samples from plant species collected in region were obtained from GenBank. (5) Data quality control was performed in CLC Bio Genomics Workbench (ver. 7.4) while bioinformatics analysis, where sequences were assigned taxonomic identity, were performed using Qiime. (6) Primary statistics contrasting animal diet included PERMANOVA and ANOSIM which utilized OTU tables from Qiime analysis.

    Article Snippet: Fecal pellets had total genomic DNA extracted using the Zymo fecal DNA kit, with the resulting DNA PCR amplified using a rbc L-mini-barcode marker set, first described in this paper.

    Techniques: Flow Cytometry, DNA Extraction, Polymerase Chain Reaction, Generated, Sequencing

    A Epifluorescence microscopic images of acridine orange stained slides, ( a ) intact soil sample and ( b ) soil sample after cell extraction. B Acridine orange staining-based microbial cell count by epifluoresence microscopy before and after cell extraction. C Electrophoresed 1 % gel showing amplified 16S rRNA gene, M molecular weight marker, lane 1 : amplified 16S rRNA gene from environmental DNA extracted by newly developed method, lane 2–7 : amplification of 16S rRNA gene from DNA extracted by published methods and kits, (it can be observed that DNA was unable to amplify by these methods). D Electrophoresed 1 % agarose gel showing, lane M : SuperMix DNA ladder (0.5 kb to 33 kb), lane 1 : environmental DNA, lane 2 : mixture of environmental DNA extracted from newly developed method and λ DNA digested with Hind III, lane 3 : λ DNA digested with Hind III and. E Polyacrylamide gel (9 %) showing: lane M molecular weight marker 100 bp, lane 2 : λ DNA digested using Hind III, lane 3 : completely digested environmental DNA extracted with newly developed method with Hae III (10 h), and lane 4 : partially digested environmental DNA extracted with newly developed method with Hae III (1 h). F Electrophoresed 1 % agarose gel showing DNA marker and extracted environmental DNA by various methods. M denotes molecular weight marker, lane 1 : showing environmental DNA extracted by newly developed method, lane 2 : high concentration of lysozyme lysis method (Gabor et al. 2003 ), lane 3 : hot detergent lysis method (Desai and Madamwar 2006 ), lane 4 : bead beating lane (Miller et al. 1999 ) 5 : NucleoSpin Soil Extract II, lane 6 : Soil gDNA isolation kit (XcelGen), lane 7 : ZR Soil Microbe DNA MiniPrep. G UV–visible absorbance spectra of environmental DNA extracted by described mentioned methods and kits

    Journal: 3 Biotech

    Article Title: An efficient and cost-effective method for DNA extraction from athalassohaline soil using a newly formulated cell extraction buffer

    doi: 10.1007/s13205-016-0383-0

    Figure Lengend Snippet: A Epifluorescence microscopic images of acridine orange stained slides, ( a ) intact soil sample and ( b ) soil sample after cell extraction. B Acridine orange staining-based microbial cell count by epifluoresence microscopy before and after cell extraction. C Electrophoresed 1 % gel showing amplified 16S rRNA gene, M molecular weight marker, lane 1 : amplified 16S rRNA gene from environmental DNA extracted by newly developed method, lane 2–7 : amplification of 16S rRNA gene from DNA extracted by published methods and kits, (it can be observed that DNA was unable to amplify by these methods). D Electrophoresed 1 % agarose gel showing, lane M : SuperMix DNA ladder (0.5 kb to 33 kb), lane 1 : environmental DNA, lane 2 : mixture of environmental DNA extracted from newly developed method and λ DNA digested with Hind III, lane 3 : λ DNA digested with Hind III and. E Polyacrylamide gel (9 %) showing: lane M molecular weight marker 100 bp, lane 2 : λ DNA digested using Hind III, lane 3 : completely digested environmental DNA extracted with newly developed method with Hae III (10 h), and lane 4 : partially digested environmental DNA extracted with newly developed method with Hae III (1 h). F Electrophoresed 1 % agarose gel showing DNA marker and extracted environmental DNA by various methods. M denotes molecular weight marker, lane 1 : showing environmental DNA extracted by newly developed method, lane 2 : high concentration of lysozyme lysis method (Gabor et al. 2003 ), lane 3 : hot detergent lysis method (Desai and Madamwar 2006 ), lane 4 : bead beating lane (Miller et al. 1999 ) 5 : NucleoSpin Soil Extract II, lane 6 : Soil gDNA isolation kit (XcelGen), lane 7 : ZR Soil Microbe DNA MiniPrep. G UV–visible absorbance spectra of environmental DNA extracted by described mentioned methods and kits

    Article Snippet: Comparison of DNA extraction method The efficiency of newly developed environmental DNA extraction method was compared with extraction from same soil samples with three commercially available kits (a) NucleoSpin® Soil (Macherely-Nagel GmbH, Germany), (b) ZR Soil Microbe DNA MiniPrep (Zymo Research, USA) and (c) XcelGen Soil gDNA isolation (based on indirect lysis) (Xcelris Genomics, India) and three widely used manual protocols (d) hot detergent lysis and column purification (Desai and Madamwar ), (e) bead beating lysis (Miller et al. ) and (f) high concentration of lysozyme/hot detergent lysis (Indirect lysis method) (Gabor et al. ).

    Techniques: Staining, Cell Counting, Microscopy, Amplification, Molecular Weight, Marker, Agarose Gel Electrophoresis, Concentration Assay, Lysis, Isolation