quick dna fecal soil microbe kit  (Zymo Research)


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    Name:
    Quick DNA Fecal Soil Microbe 96 Kit
    Description:
    The Quick DNA Fecal Soil Microbe 96 Kit is designed for the simple and rapid isolation of inhibitor free PCR quality host cell and microbial DNA from a variety of sample sources including humans birds rats mice cattle etc The procedure is easy and can be completed in minutes fecal samples are rapidly and efficiently lysed by bead beating with our state of the art ultra high density BashingBeads Zymo Spin technology is then used to isolate the DNA which is subsequently filtered to remove humic acids polyphenols that can inhibit PCR Eluted DNA is ideal for downstream molecular based applications including PCR arrays genotyping methylation detection etc
    Catalog Number:
    d6011
    Product Aliases:
    ZR-96 Fecal DNA Kit
    Price:
    None
    Applications:
    DNA Purification
    Size:
    2 x 96 units
    Category:
    Life Science Reagents and Media
    Buy from Supplier


    Structured Review

    Zymo Research quick dna fecal soil microbe kit
    Quick DNA Fecal Soil Microbe 96 Kit
    The Quick DNA Fecal Soil Microbe 96 Kit is designed for the simple and rapid isolation of inhibitor free PCR quality host cell and microbial DNA from a variety of sample sources including humans birds rats mice cattle etc The procedure is easy and can be completed in minutes fecal samples are rapidly and efficiently lysed by bead beating with our state of the art ultra high density BashingBeads Zymo Spin technology is then used to isolate the DNA which is subsequently filtered to remove humic acids polyphenols that can inhibit PCR Eluted DNA is ideal for downstream molecular based applications including PCR arrays genotyping methylation detection etc
    https://www.bioz.com/result/quick dna fecal soil microbe kit/product/Zymo Research
    Average 95 stars, based on 89 article reviews
    Price from $9.99 to $1999.99
    quick dna fecal soil microbe kit - by Bioz Stars, 2020-02
    95/100 stars

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    Related Articles

    DNA Extraction:

    Article Title: Antibiotic-Induced Perturbations Are Manifested in the Dominant Intestinal Bacterial Phyla of Atlantic Salmon
    Article Snippet: .. Bacterial DNA Isolation, PCR Amplification, 16S rRNA Gene Amplicon Library Preparation and Sequencing We first extracted the total genomic DNA from the fish and environmental samples using the Quick-DNA™ Fecal/Soil Microbe 96 kit (Zymo Research, Irvine, CA, USA). .. The quality of the isolated DNA was checked on 1.2% (w/v) agarose gel and quantified using a Qubit 3.0 fluorometer (Life Technologies, Carlsbad, CA, USA).

    Article Title: Fumonisin-Exposure Impairs Age-Related Ecological Succession of Bacterial Species in Weaned Pig Gut Microbiota
    Article Snippet: .. DNA Extraction and PCR Total genomic DNA was extracted from 0.5 g of fecal sample, combining a mechanical lysis with a TissueLyser II instrument (Qiagen, Hilden, Germany) and the Quick-DNA™ Fecal/Soil Microbe 96 Kit Zymo Research, Irvine, CA, USA), according to the manufacturer’s instructions [ ]. .. The quality and quantity of DNA extracts were checked using a NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA).

    Article Title: Bacterial Communities in the Alpaca Gastrointestinal Tract Vary With Diet and Body Site
    Article Snippet: Paragraph title: DNA Extraction ... A preliminary analysis showed no differences in the unweighted Unifrac scores of identical samples when DNA was isolated using a different kit [ZR-96 Fecal DNA KitTM (Zymo Research, Irvine, CA, United States)] that had superior economy of time and cost.

    Article Title: Monitoring Seasonal Changes in Winery-Resident Microbiota
    Article Snippet: .. The cotton tip of each swab was aseptically removed from the shaft and placed directly into the 96-well lysis plate provided in the ZR-96 Fecal DNA extraction kit (Zymo Research, Irvine, CA). .. DNA was extracted using the standard protocol for the ZR-96 kit, with bead beating with a Genogrinder high-throughput tissue homogenizer (SPEX SamplePrep, Metuchen, NJ) and stored at –20°C until further processing.

    Article Title: Surface Microbes in the Neonatal Intensive Care Unit: Changes with Routine Cleaning and over Time
    Article Snippet: .. The cotton tip of each swab was aseptically removed from the shaft and placed directly into the 96-well lysis plate provided in the ZR-96 fecal DNA extraction kit (Zymo Research, Irvine, CA). .. DNA was extracted using the standard protocol for the ZR-96 kit, with bead beating done with a Geno/Grinder high-throughput tissue homogenizer (SPEX SamplePrep, Metuchen, NJ), and stored at −20°C until further processing.

    Article Title: Facility-Specific "House" Microbiome Drives Microbial Landscapes of Artisan Cheesemaking Plants
    Article Snippet: .. The cotton tip of each swab was aseptically removed from the shaft and placed directly into the 96-well lysis plate provided in the ZR-96 Fecal DNA extraction kit (Zymo Research, Irvine, CA). .. DNA was extracted by using the standard protocol for the ZR-96 kit, with bead beating with a Genogrinder high-throughput tissue homogenizer (SPEX SamplePrep, Metuchen, NJ) and stored at −20°C until further processing.

    Article Title: Seasonal, spatial, and maternal effects on gut microbiome in wild red squirrels
    Article Snippet: Paragraph title: Fecal sample collection, DNA extraction, and 16S rRNA sequencing ... We extracted DNA from fecal samples in a 96-well format using the ZR-96 Fecal DNA Kits (Zymo Research, Orange, CA) following the manufacturer’s protocol.

    Article Title: Lactobacillus Dominate in the Intestine of Atlantic Salmon Fed Dietary Probiotics
    Article Snippet: .. DNA Extraction and PCR Amplification of Bacterial 16S rRNA Gene for Illumina MiSeq Amplicon Sequencing Genomic DNA was extracted from the content, mucus and biofilm samples using the Quick-DNA™ Fecal/Soil Microbe 96 kit (Zymo Research, Irvine, CA, USA) following the manufacturer's protocol. .. Metagenomic DNA Isolation kit for water (Epicenter Biotechnologies, Madison, WI, USA) was employed to extract the genomic DNA from the water samples.

    Article Title: A comparative study of the gut microbiota in immune-mediated inflammatory diseases—does a common dysbiosis exist?
    Article Snippet: Paragraph title: DNA extraction, library preparation, and 16S rRNA gene amplicon sequencing ... DNA was isolated using the ZR-96 Fecal DNA Kit (Zymo Research, Irvine, CA) following a validated protocol [ ].

    Article Title: Fumonisin-Exposure Impairs Age-Related Ecological Succession of Bacterial Species in Weaned Pig Gut Microbiota
    Article Snippet: Paragraph title: 5.3. DNA Extraction and PCR ... Total genomic DNA was extracted from 0.5 g of fecal sample, combining a mechanical lysis with a TissueLyser II instrument (Qiagen, Hilden, Germany) and the Quick-DNA™ Fecal/Soil Microbe 96 Kit Zymo Research, Irvine, CA, USA), according to the manufacturer’s instructions [ ].

    Article Title: Lactobacillus Dominate in the Intestine of Atlantic Salmon Fed Dietary Probiotics
    Article Snippet: Paragraph title: DNA Extraction and PCR Amplification of Bacterial 16S rRNA Gene for Illumina MiSeq Amplicon Sequencing ... Genomic DNA was extracted from the content, mucus and biofilm samples using the Quick-DNA™ Fecal/Soil Microbe 96 kit (Zymo Research, Irvine, CA, USA) following the manufacturer's protocol.

    Article Title: Macroalga-Derived Alginate Oligosaccharide Alters Intestinal Bacteria of Atlantic Salmon
    Article Snippet: .. DNA Extraction, PCR Amplification, Amplicon Library Construction, and Sequencing Genomic DNA was extracted from all samples (except water filter samples) using the Quick-DNATM Fecal/Soil Microbe 96 kit (Zymo Research, Irvine, CA, United States). .. Genomic DNA from the water filter samples was extracted using Metagenomic DNA Isolation kit for water (Epicentre Biotechnologies, Madison, WI, United States), according to the manufacturer’s instructions.

    Amplification:

    Article Title: Antibiotic-Induced Perturbations Are Manifested in the Dominant Intestinal Bacterial Phyla of Atlantic Salmon
    Article Snippet: .. Bacterial DNA Isolation, PCR Amplification, 16S rRNA Gene Amplicon Library Preparation and Sequencing We first extracted the total genomic DNA from the fish and environmental samples using the Quick-DNA™ Fecal/Soil Microbe 96 kit (Zymo Research, Irvine, CA, USA). .. The quality of the isolated DNA was checked on 1.2% (w/v) agarose gel and quantified using a Qubit 3.0 fluorometer (Life Technologies, Carlsbad, CA, USA).

    Article Title: Fumonisin-Exposure Impairs Age-Related Ecological Succession of Bacterial Species in Weaned Pig Gut Microbiota
    Article Snippet: DNA Extraction and PCR Total genomic DNA was extracted from 0.5 g of fecal sample, combining a mechanical lysis with a TissueLyser II instrument (Qiagen, Hilden, Germany) and the Quick-DNA™ Fecal/Soil Microbe 96 Kit Zymo Research, Irvine, CA, USA), according to the manufacturer’s instructions [ ]. .. The V3–V4 regions of 16S rRNA genes of samples were amplified from purified genomic DNA with the primers F343 (5′-CTTTCCCTACACGACGCTCTTCCGATCTTACGGRAGGCAGCAG-3′; [ ]) and reverse R784 (5′-GGAGTTCAGACGTGTGCTCTTCCGATCTTACCAGGGTATCTAATC CT-3′; [ ]).

    Article Title: Sustained Captopril‐Induced Reduction in Blood Pressure Is Associated With Alterations in Gut‐Brain Axis in the Spontaneously Hypertensive Rat
    Article Snippet: Briefly, fecal DNA was extracted by using quick‐DNA fecal/soil microbe kit (Zymoresearch, Irvine, CA). .. V4 to V5 variable region was amplified in each fecal sample using a pair of primers containing Illumina standard adaptors and unique barcode combination.

    Article Title: Seasonal, spatial, and maternal effects on gut microbiome in wild red squirrels
    Article Snippet: We extracted DNA from fecal samples in a 96-well format using the ZR-96 Fecal DNA Kits (Zymo Research, Orange, CA) following the manufacturer’s protocol. .. The V1–V3 hypervariable regions of the 16S rRNA gene were amplified using two universal primers 27F (5’-AGRGTTTGATCMTGGCTCAG-3′) and 534R (5’-TTACCGCGGCTGCTGGCAC-3′).

    Article Title: Lactobacillus Dominate in the Intestine of Atlantic Salmon Fed Dietary Probiotics
    Article Snippet: .. DNA Extraction and PCR Amplification of Bacterial 16S rRNA Gene for Illumina MiSeq Amplicon Sequencing Genomic DNA was extracted from the content, mucus and biofilm samples using the Quick-DNA™ Fecal/Soil Microbe 96 kit (Zymo Research, Irvine, CA, USA) following the manufacturer's protocol. .. Metagenomic DNA Isolation kit for water (Epicenter Biotechnologies, Madison, WI, USA) was employed to extract the genomic DNA from the water samples.

    Article Title: A comparative study of the gut microbiota in immune-mediated inflammatory diseases—does a common dysbiosis exist?
    Article Snippet: Paragraph title: DNA extraction, library preparation, and 16S rRNA gene amplicon sequencing ... DNA was isolated using the ZR-96 Fecal DNA Kit (Zymo Research, Irvine, CA) following a validated protocol [ ].

    Article Title: Fumonisin-Exposure Impairs Age-Related Ecological Succession of Bacterial Species in Weaned Pig Gut Microbiota
    Article Snippet: Total genomic DNA was extracted from 0.5 g of fecal sample, combining a mechanical lysis with a TissueLyser II instrument (Qiagen, Hilden, Germany) and the Quick-DNA™ Fecal/Soil Microbe 96 Kit Zymo Research, Irvine, CA, USA), according to the manufacturer’s instructions [ ]. .. The V3–V4 regions of 16S rRNA genes of samples were amplified from purified genomic DNA with the primers F343 (5′-CTTTCCCTACACGACGCTCTTCCGATCTTACGGRAGGCAGCAG-3′; [ ]) and reverse R784 (5′-GGAGTTCAGACGTGTGCTCTTCCGATCTTACCAGGGTATCTAATC CT-3′; [ ]).

    Article Title: Lactobacillus Dominate in the Intestine of Atlantic Salmon Fed Dietary Probiotics
    Article Snippet: Paragraph title: DNA Extraction and PCR Amplification of Bacterial 16S rRNA Gene for Illumina MiSeq Amplicon Sequencing ... Genomic DNA was extracted from the content, mucus and biofilm samples using the Quick-DNA™ Fecal/Soil Microbe 96 kit (Zymo Research, Irvine, CA, USA) following the manufacturer's protocol.

    Article Title: Macroalga-Derived Alginate Oligosaccharide Alters Intestinal Bacteria of Atlantic Salmon
    Article Snippet: .. DNA Extraction, PCR Amplification, Amplicon Library Construction, and Sequencing Genomic DNA was extracted from all samples (except water filter samples) using the Quick-DNATM Fecal/Soil Microbe 96 kit (Zymo Research, Irvine, CA, United States). .. Genomic DNA from the water filter samples was extracted using Metagenomic DNA Isolation kit for water (Epicentre Biotechnologies, Madison, WI, United States), according to the manufacturer’s instructions.

    Agarose Gel Electrophoresis:

    Article Title: Antibiotic-Induced Perturbations Are Manifested in the Dominant Intestinal Bacterial Phyla of Atlantic Salmon
    Article Snippet: Bacterial DNA Isolation, PCR Amplification, 16S rRNA Gene Amplicon Library Preparation and Sequencing We first extracted the total genomic DNA from the fish and environmental samples using the Quick-DNA™ Fecal/Soil Microbe 96 kit (Zymo Research, Irvine, CA, USA). .. The quality of the isolated DNA was checked on 1.2% (w/v) agarose gel and quantified using a Qubit 3.0 fluorometer (Life Technologies, Carlsbad, CA, USA).

    Article Title: Macroalga-Derived Alginate Oligosaccharide Alters Intestinal Bacteria of Atlantic Salmon
    Article Snippet: DNA Extraction, PCR Amplification, Amplicon Library Construction, and Sequencing Genomic DNA was extracted from all samples (except water filter samples) using the Quick-DNATM Fecal/Soil Microbe 96 kit (Zymo Research, Irvine, CA, United States). .. The quality of the extracted DNA was checked on 1.2% (w/v) agarose gel and the DNA concentration was quantified using the Qubit 3.0 fluorometer (Life Technologies, Carlsbad, United States).

    Polymerase Chain Reaction:

    Article Title: Antibiotic-Induced Perturbations Are Manifested in the Dominant Intestinal Bacterial Phyla of Atlantic Salmon
    Article Snippet: .. Bacterial DNA Isolation, PCR Amplification, 16S rRNA Gene Amplicon Library Preparation and Sequencing We first extracted the total genomic DNA from the fish and environmental samples using the Quick-DNA™ Fecal/Soil Microbe 96 kit (Zymo Research, Irvine, CA, USA). .. The quality of the isolated DNA was checked on 1.2% (w/v) agarose gel and quantified using a Qubit 3.0 fluorometer (Life Technologies, Carlsbad, CA, USA).

    Article Title: Fumonisin-Exposure Impairs Age-Related Ecological Succession of Bacterial Species in Weaned Pig Gut Microbiota
    Article Snippet: .. DNA Extraction and PCR Total genomic DNA was extracted from 0.5 g of fecal sample, combining a mechanical lysis with a TissueLyser II instrument (Qiagen, Hilden, Germany) and the Quick-DNA™ Fecal/Soil Microbe 96 Kit Zymo Research, Irvine, CA, USA), according to the manufacturer’s instructions [ ]. .. The quality and quantity of DNA extracts were checked using a NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA).

    Article Title: Seasonal, spatial, and maternal effects on gut microbiome in wild red squirrels
    Article Snippet: We extracted DNA from fecal samples in a 96-well format using the ZR-96 Fecal DNA Kits (Zymo Research, Orange, CA) following the manufacturer’s protocol. .. We added a unique 8 bp barcode to each primer to tag the samples and used a 50-uL reaction for each PCR amplification by QIAGEN Taq polymerase (Qiagen Inc., CA).

    Article Title: Lactobacillus Dominate in the Intestine of Atlantic Salmon Fed Dietary Probiotics
    Article Snippet: .. DNA Extraction and PCR Amplification of Bacterial 16S rRNA Gene for Illumina MiSeq Amplicon Sequencing Genomic DNA was extracted from the content, mucus and biofilm samples using the Quick-DNA™ Fecal/Soil Microbe 96 kit (Zymo Research, Irvine, CA, USA) following the manufacturer's protocol. .. Metagenomic DNA Isolation kit for water (Epicenter Biotechnologies, Madison, WI, USA) was employed to extract the genomic DNA from the water samples.

    Article Title: Fumonisin-Exposure Impairs Age-Related Ecological Succession of Bacterial Species in Weaned Pig Gut Microbiota
    Article Snippet: Paragraph title: 5.3. DNA Extraction and PCR ... Total genomic DNA was extracted from 0.5 g of fecal sample, combining a mechanical lysis with a TissueLyser II instrument (Qiagen, Hilden, Germany) and the Quick-DNA™ Fecal/Soil Microbe 96 Kit Zymo Research, Irvine, CA, USA), according to the manufacturer’s instructions [ ].

    Article Title: Lactobacillus Dominate in the Intestine of Atlantic Salmon Fed Dietary Probiotics
    Article Snippet: Paragraph title: DNA Extraction and PCR Amplification of Bacterial 16S rRNA Gene for Illumina MiSeq Amplicon Sequencing ... Genomic DNA was extracted from the content, mucus and biofilm samples using the Quick-DNA™ Fecal/Soil Microbe 96 kit (Zymo Research, Irvine, CA, USA) following the manufacturer's protocol.

    Article Title: Macroalga-Derived Alginate Oligosaccharide Alters Intestinal Bacteria of Atlantic Salmon
    Article Snippet: .. DNA Extraction, PCR Amplification, Amplicon Library Construction, and Sequencing Genomic DNA was extracted from all samples (except water filter samples) using the Quick-DNATM Fecal/Soil Microbe 96 kit (Zymo Research, Irvine, CA, United States). .. Genomic DNA from the water filter samples was extracted using Metagenomic DNA Isolation kit for water (Epicentre Biotechnologies, Madison, WI, United States), according to the manufacturer’s instructions.

    Isolation:

    Article Title: Antibiotic-Induced Perturbations Are Manifested in the Dominant Intestinal Bacterial Phyla of Atlantic Salmon
    Article Snippet: Bacterial DNA Isolation, PCR Amplification, 16S rRNA Gene Amplicon Library Preparation and Sequencing We first extracted the total genomic DNA from the fish and environmental samples using the Quick-DNA™ Fecal/Soil Microbe 96 kit (Zymo Research, Irvine, CA, USA). .. The quality of the isolated DNA was checked on 1.2% (w/v) agarose gel and quantified using a Qubit 3.0 fluorometer (Life Technologies, Carlsbad, CA, USA).

    Article Title: Bacterial Communities in the Alpaca Gastrointestinal Tract Vary With Diet and Body Site
    Article Snippet: .. A preliminary analysis showed no differences in the unweighted Unifrac scores of identical samples when DNA was isolated using a different kit [ZR-96 Fecal DNA KitTM (Zymo Research, Irvine, CA, United States)] that had superior economy of time and cost. .. For the preliminary analysis, DNA was extracted from four paired C1 samples by each of the Zymo ZR Fecal DNA MiniPrep and PowerFecal® DNA extraction kits.

    Article Title: A comparative study of the gut microbiota in immune-mediated inflammatory diseases—does a common dysbiosis exist?
    Article Snippet: .. DNA was isolated using the ZR-96 Fecal DNA Kit (Zymo Research, Irvine, CA) following a validated protocol [ ]. .. The Illumina MiSeq sequencing library preparation protocol for 16S rRNA gene amplicons was followed with modifications [ ].

    Sequencing:

    Article Title: Antibiotic-Induced Perturbations Are Manifested in the Dominant Intestinal Bacterial Phyla of Atlantic Salmon
    Article Snippet: .. Bacterial DNA Isolation, PCR Amplification, 16S rRNA Gene Amplicon Library Preparation and Sequencing We first extracted the total genomic DNA from the fish and environmental samples using the Quick-DNA™ Fecal/Soil Microbe 96 kit (Zymo Research, Irvine, CA, USA). .. The quality of the isolated DNA was checked on 1.2% (w/v) agarose gel and quantified using a Qubit 3.0 fluorometer (Life Technologies, Carlsbad, CA, USA).

    Article Title: Facility-Specific "House" Microbiome Drives Microbial Landscapes of Artisan Cheesemaking Plants
    Article Snippet: The cotton tip of each swab was aseptically removed from the shaft and placed directly into the 96-well lysis plate provided in the ZR-96 Fecal DNA extraction kit (Zymo Research, Irvine, CA). .. A complete list of each sample collected is presented in Tables S1 and S2 in the supplemental material as mapping files formatted for sequencing analysis directly in QIIME ( ).

    Article Title: Sustained Captopril‐Induced Reduction in Blood Pressure Is Associated With Alterations in Gut‐Brain Axis in the Spontaneously Hypertensive Rat
    Article Snippet: Paragraph title: 16S rRNA Gene Sequencing ... Briefly, fecal DNA was extracted by using quick‐DNA fecal/soil microbe kit (Zymoresearch, Irvine, CA).

    Article Title: Seasonal, spatial, and maternal effects on gut microbiome in wild red squirrels
    Article Snippet: Paragraph title: Fecal sample collection, DNA extraction, and 16S rRNA sequencing ... We extracted DNA from fecal samples in a 96-well format using the ZR-96 Fecal DNA Kits (Zymo Research, Orange, CA) following the manufacturer’s protocol.

    Article Title: Lactobacillus Dominate in the Intestine of Atlantic Salmon Fed Dietary Probiotics
    Article Snippet: .. DNA Extraction and PCR Amplification of Bacterial 16S rRNA Gene for Illumina MiSeq Amplicon Sequencing Genomic DNA was extracted from the content, mucus and biofilm samples using the Quick-DNA™ Fecal/Soil Microbe 96 kit (Zymo Research, Irvine, CA, USA) following the manufacturer's protocol. .. Metagenomic DNA Isolation kit for water (Epicenter Biotechnologies, Madison, WI, USA) was employed to extract the genomic DNA from the water samples.

    Article Title: A comparative study of the gut microbiota in immune-mediated inflammatory diseases—does a common dysbiosis exist?
    Article Snippet: Paragraph title: DNA extraction, library preparation, and 16S rRNA gene amplicon sequencing ... DNA was isolated using the ZR-96 Fecal DNA Kit (Zymo Research, Irvine, CA) following a validated protocol [ ].

    Article Title: Induction of Subacute Ruminal Acidosis Affects the Ruminal Microbiome and Epithelium
    Article Snippet: DNA from the liquid fraction (50 mL) was extracted using the ZR-96 Fecal DNA Kit (ZYMO Research, Irvine, CA, USA), which included a bead-beating step for mechanical lysis of bacterial cell walls. .. Extracted DNA from the solid fraction was standardized to 8 ng/μL for quantitative PCR (qPCR) and 20 ng/μL for Illumina sequencing.

    Article Title: Lactobacillus Dominate in the Intestine of Atlantic Salmon Fed Dietary Probiotics
    Article Snippet: Paragraph title: DNA Extraction and PCR Amplification of Bacterial 16S rRNA Gene for Illumina MiSeq Amplicon Sequencing ... Genomic DNA was extracted from the content, mucus and biofilm samples using the Quick-DNA™ Fecal/Soil Microbe 96 kit (Zymo Research, Irvine, CA, USA) following the manufacturer's protocol.

    Article Title: Macroalga-Derived Alginate Oligosaccharide Alters Intestinal Bacteria of Atlantic Salmon
    Article Snippet: .. DNA Extraction, PCR Amplification, Amplicon Library Construction, and Sequencing Genomic DNA was extracted from all samples (except water filter samples) using the Quick-DNATM Fecal/Soil Microbe 96 kit (Zymo Research, Irvine, CA, United States). .. Genomic DNA from the water filter samples was extracted using Metagenomic DNA Isolation kit for water (Epicentre Biotechnologies, Madison, WI, United States), according to the manufacturer’s instructions.

    Spectrophotometry:

    Article Title: Fumonisin-Exposure Impairs Age-Related Ecological Succession of Bacterial Species in Weaned Pig Gut Microbiota
    Article Snippet: DNA Extraction and PCR Total genomic DNA was extracted from 0.5 g of fecal sample, combining a mechanical lysis with a TissueLyser II instrument (Qiagen, Hilden, Germany) and the Quick-DNA™ Fecal/Soil Microbe 96 Kit Zymo Research, Irvine, CA, USA), according to the manufacturer’s instructions [ ]. .. The quality and quantity of DNA extracts were checked using a NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA).

    Article Title: Fumonisin-Exposure Impairs Age-Related Ecological Succession of Bacterial Species in Weaned Pig Gut Microbiota
    Article Snippet: Total genomic DNA was extracted from 0.5 g of fecal sample, combining a mechanical lysis with a TissueLyser II instrument (Qiagen, Hilden, Germany) and the Quick-DNA™ Fecal/Soil Microbe 96 Kit Zymo Research, Irvine, CA, USA), according to the manufacturer’s instructions [ ]. .. The quality and quantity of DNA extracts were checked using a NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA).

    Purification:

    Article Title: Fumonisin-Exposure Impairs Age-Related Ecological Succession of Bacterial Species in Weaned Pig Gut Microbiota
    Article Snippet: DNA Extraction and PCR Total genomic DNA was extracted from 0.5 g of fecal sample, combining a mechanical lysis with a TissueLyser II instrument (Qiagen, Hilden, Germany) and the Quick-DNA™ Fecal/Soil Microbe 96 Kit Zymo Research, Irvine, CA, USA), according to the manufacturer’s instructions [ ]. .. The V3–V4 regions of 16S rRNA genes of samples were amplified from purified genomic DNA with the primers F343 (5′-CTTTCCCTACACGACGCTCTTCCGATCTTACGGRAGGCAGCAG-3′; [ ]) and reverse R784 (5′-GGAGTTCAGACGTGTGCTCTTCCGATCTTACCAGGGTATCTAATC CT-3′; [ ]).

    Article Title: Sustained Captopril‐Induced Reduction in Blood Pressure Is Associated With Alterations in Gut‐Brain Axis in the Spontaneously Hypertensive Rat
    Article Snippet: Briefly, fecal DNA was extracted by using quick‐DNA fecal/soil microbe kit (Zymoresearch, Irvine, CA). .. Amplicons were purified by a QIAquick gel extraction kit (QIAGEN, Hilden, Germany) and pooled for a library with an equal amount of each amplicon.

    Article Title: Fumonisin-Exposure Impairs Age-Related Ecological Succession of Bacterial Species in Weaned Pig Gut Microbiota
    Article Snippet: Total genomic DNA was extracted from 0.5 g of fecal sample, combining a mechanical lysis with a TissueLyser II instrument (Qiagen, Hilden, Germany) and the Quick-DNA™ Fecal/Soil Microbe 96 Kit Zymo Research, Irvine, CA, USA), according to the manufacturer’s instructions [ ]. .. The V3–V4 regions of 16S rRNA genes of samples were amplified from purified genomic DNA with the primers F343 (5′-CTTTCCCTACACGACGCTCTTCCGATCTTACGGRAGGCAGCAG-3′; [ ]) and reverse R784 (5′-GGAGTTCAGACGTGTGCTCTTCCGATCTTACCAGGGTATCTAATC CT-3′; [ ]).

    Real-time Polymerase Chain Reaction:

    Article Title: Sustained Captopril‐Induced Reduction in Blood Pressure Is Associated With Alterations in Gut‐Brain Axis in the Spontaneously Hypertensive Rat
    Article Snippet: Briefly, fecal DNA was extracted by using quick‐DNA fecal/soil microbe kit (Zymoresearch, Irvine, CA). .. The library was quantified and quality checked by Qubit (Thermo Fisher Scientific, Waltham, MA) and qPCR (Kapa Biosystems, Wilmington, MA), before Miseq sequencing with a 2 by 250 base pairs v2 kit (Illumina, San Diego, CA).

    Article Title: Induction of Subacute Ruminal Acidosis Affects the Ruminal Microbiome and Epithelium
    Article Snippet: DNA from the liquid fraction (50 mL) was extracted using the ZR-96 Fecal DNA Kit (ZYMO Research, Irvine, CA, USA), which included a bead-beating step for mechanical lysis of bacterial cell walls. .. Extracted DNA from the solid fraction was standardized to 8 ng/μL for quantitative PCR (qPCR) and 20 ng/μL for Illumina sequencing.

    Concentration Assay:

    Article Title: Lactobacillus Dominate in the Intestine of Atlantic Salmon Fed Dietary Probiotics
    Article Snippet: DNA Extraction and PCR Amplification of Bacterial 16S rRNA Gene for Illumina MiSeq Amplicon Sequencing Genomic DNA was extracted from the content, mucus and biofilm samples using the Quick-DNA™ Fecal/Soil Microbe 96 kit (Zymo Research, Irvine, CA, USA) following the manufacturer's protocol. .. Qubit 3.0 fluorometer (Life Technologies, Carlsbad, USA) was employed to quantify the concentration of DNA.

    Article Title: Lactobacillus Dominate in the Intestine of Atlantic Salmon Fed Dietary Probiotics
    Article Snippet: Genomic DNA was extracted from the content, mucus and biofilm samples using the Quick-DNA™ Fecal/Soil Microbe 96 kit (Zymo Research, Irvine, CA, USA) following the manufacturer's protocol. .. Qubit 3.0 fluorometer (Life Technologies, Carlsbad, USA) was employed to quantify the concentration of DNA.

    Article Title: Macroalga-Derived Alginate Oligosaccharide Alters Intestinal Bacteria of Atlantic Salmon
    Article Snippet: DNA Extraction, PCR Amplification, Amplicon Library Construction, and Sequencing Genomic DNA was extracted from all samples (except water filter samples) using the Quick-DNATM Fecal/Soil Microbe 96 kit (Zymo Research, Irvine, CA, United States). .. The quality of the extracted DNA was checked on 1.2% (w/v) agarose gel and the DNA concentration was quantified using the Qubit 3.0 fluorometer (Life Technologies, Carlsbad, United States).

    Lysis:

    Article Title: Fumonisin-Exposure Impairs Age-Related Ecological Succession of Bacterial Species in Weaned Pig Gut Microbiota
    Article Snippet: .. DNA Extraction and PCR Total genomic DNA was extracted from 0.5 g of fecal sample, combining a mechanical lysis with a TissueLyser II instrument (Qiagen, Hilden, Germany) and the Quick-DNA™ Fecal/Soil Microbe 96 Kit Zymo Research, Irvine, CA, USA), according to the manufacturer’s instructions [ ]. .. The quality and quantity of DNA extracts were checked using a NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA).

    Article Title: Monitoring Seasonal Changes in Winery-Resident Microbiota
    Article Snippet: .. The cotton tip of each swab was aseptically removed from the shaft and placed directly into the 96-well lysis plate provided in the ZR-96 Fecal DNA extraction kit (Zymo Research, Irvine, CA). .. DNA was extracted using the standard protocol for the ZR-96 kit, with bead beating with a Genogrinder high-throughput tissue homogenizer (SPEX SamplePrep, Metuchen, NJ) and stored at –20°C until further processing.

    Article Title: Surface Microbes in the Neonatal Intensive Care Unit: Changes with Routine Cleaning and over Time
    Article Snippet: .. The cotton tip of each swab was aseptically removed from the shaft and placed directly into the 96-well lysis plate provided in the ZR-96 fecal DNA extraction kit (Zymo Research, Irvine, CA). .. DNA was extracted using the standard protocol for the ZR-96 kit, with bead beating done with a Geno/Grinder high-throughput tissue homogenizer (SPEX SamplePrep, Metuchen, NJ), and stored at −20°C until further processing.

    Article Title: Facility-Specific "House" Microbiome Drives Microbial Landscapes of Artisan Cheesemaking Plants
    Article Snippet: .. The cotton tip of each swab was aseptically removed from the shaft and placed directly into the 96-well lysis plate provided in the ZR-96 Fecal DNA extraction kit (Zymo Research, Irvine, CA). .. DNA was extracted by using the standard protocol for the ZR-96 kit, with bead beating with a Genogrinder high-throughput tissue homogenizer (SPEX SamplePrep, Metuchen, NJ) and stored at −20°C until further processing.

    Article Title: Fumonisin-Exposure Impairs Age-Related Ecological Succession of Bacterial Species in Weaned Pig Gut Microbiota
    Article Snippet: .. Total genomic DNA was extracted from 0.5 g of fecal sample, combining a mechanical lysis with a TissueLyser II instrument (Qiagen, Hilden, Germany) and the Quick-DNA™ Fecal/Soil Microbe 96 Kit Zymo Research, Irvine, CA, USA), according to the manufacturer’s instructions [ ]. .. The quality and quantity of DNA extracts were checked using a NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA).

    Article Title: Induction of Subacute Ruminal Acidosis Affects the Ruminal Microbiome and Epithelium
    Article Snippet: .. DNA from the liquid fraction (50 mL) was extracted using the ZR-96 Fecal DNA Kit (ZYMO Research, Irvine, CA, USA), which included a bead-beating step for mechanical lysis of bacterial cell walls. .. Extracted DNA from the solid fraction was standardized to 8 ng/μL for quantitative PCR (qPCR) and 20 ng/μL for Illumina sequencing.

    High Throughput Screening Assay:

    Article Title: Monitoring Seasonal Changes in Winery-Resident Microbiota
    Article Snippet: The cotton tip of each swab was aseptically removed from the shaft and placed directly into the 96-well lysis plate provided in the ZR-96 Fecal DNA extraction kit (Zymo Research, Irvine, CA). .. DNA was extracted using the standard protocol for the ZR-96 kit, with bead beating with a Genogrinder high-throughput tissue homogenizer (SPEX SamplePrep, Metuchen, NJ) and stored at –20°C until further processing.

    Article Title: Surface Microbes in the Neonatal Intensive Care Unit: Changes with Routine Cleaning and over Time
    Article Snippet: The cotton tip of each swab was aseptically removed from the shaft and placed directly into the 96-well lysis plate provided in the ZR-96 fecal DNA extraction kit (Zymo Research, Irvine, CA). .. DNA was extracted using the standard protocol for the ZR-96 kit, with bead beating done with a Geno/Grinder high-throughput tissue homogenizer (SPEX SamplePrep, Metuchen, NJ), and stored at −20°C until further processing.

    Article Title: Facility-Specific "House" Microbiome Drives Microbial Landscapes of Artisan Cheesemaking Plants
    Article Snippet: The cotton tip of each swab was aseptically removed from the shaft and placed directly into the 96-well lysis plate provided in the ZR-96 Fecal DNA extraction kit (Zymo Research, Irvine, CA). .. DNA was extracted by using the standard protocol for the ZR-96 kit, with bead beating with a Genogrinder high-throughput tissue homogenizer (SPEX SamplePrep, Metuchen, NJ) and stored at −20°C until further processing.

    Gel Extraction:

    Article Title: Sustained Captopril‐Induced Reduction in Blood Pressure Is Associated With Alterations in Gut‐Brain Axis in the Spontaneously Hypertensive Rat
    Article Snippet: Briefly, fecal DNA was extracted by using quick‐DNA fecal/soil microbe kit (Zymoresearch, Irvine, CA). .. Amplicons were purified by a QIAquick gel extraction kit (QIAGEN, Hilden, Germany) and pooled for a library with an equal amount of each amplicon.

    Sampling:

    Article Title: Induction of Subacute Ruminal Acidosis Affects the Ruminal Microbiome and Epithelium
    Article Snippet: Paragraph title: Rumen Sampling and Nucleic Acid Extraction ... DNA from the liquid fraction (50 mL) was extracted using the ZR-96 Fecal DNA Kit (ZYMO Research, Irvine, CA, USA), which included a bead-beating step for mechanical lysis of bacterial cell walls.

    Fluorescence In Situ Hybridization:

    Article Title: Antibiotic-Induced Perturbations Are Manifested in the Dominant Intestinal Bacterial Phyla of Atlantic Salmon
    Article Snippet: .. Bacterial DNA Isolation, PCR Amplification, 16S rRNA Gene Amplicon Library Preparation and Sequencing We first extracted the total genomic DNA from the fish and environmental samples using the Quick-DNA™ Fecal/Soil Microbe 96 kit (Zymo Research, Irvine, CA, USA). .. The quality of the isolated DNA was checked on 1.2% (w/v) agarose gel and quantified using a Qubit 3.0 fluorometer (Life Technologies, Carlsbad, CA, USA).

    Environmental Sampling:

    Article Title: Antibiotic-Induced Perturbations Are Manifested in the Dominant Intestinal Bacterial Phyla of Atlantic Salmon
    Article Snippet: .. Bacterial DNA Isolation, PCR Amplification, 16S rRNA Gene Amplicon Library Preparation and Sequencing We first extracted the total genomic DNA from the fish and environmental samples using the Quick-DNA™ Fecal/Soil Microbe 96 kit (Zymo Research, Irvine, CA, USA). .. The quality of the isolated DNA was checked on 1.2% (w/v) agarose gel and quantified using a Qubit 3.0 fluorometer (Life Technologies, Carlsbad, CA, USA).

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    Zymo Research quick dna fecal soil microbe microprep kit
    Quick Dna Fecal Soil Microbe Microprep Kit, supplied by Zymo Research, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Zymo Research quick dna fecal soil microbe miniprep kit
    Quick Dna Fecal Soil Microbe Miniprep Kit, supplied by Zymo Research, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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