q5  (New England Biolabs)


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    Name:
    NEBNext High Fidelity 2X PCR Master Mix
    Description:
    NEBNext High Fidelity 2X PCR Master Mix 250 rxns
    Catalog Number:
    m0541l
    Price:
    360
    Size:
    250 rxns
    Category:
    Thermostable DNA Polymerases
    Buy from Supplier


    Structured Review

    New England Biolabs q5
    NEBNext High Fidelity 2X PCR Master Mix
    NEBNext High Fidelity 2X PCR Master Mix 250 rxns
    https://www.bioz.com/result/q5/product/New England Biolabs
    Average 97 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    q5 - by Bioz Stars, 2021-02
    97/100 stars

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    Related Articles

    Amplification:

    Article Title: Cooperative interactions enable singular olfactory receptor expression in mouse olfactory neurons
    Article Snippet: .. The transposed DNA was then amplified using barcoded primers and NEBNext High Fidelity 2x PCR Master Mix (NEB). .. Amplified libraries were purified using Ampure XP beads (Beckman Coulter) at a ratio of 1.6 ul of beads per 1 uL of library and eluted in 30 uL of elution buffer (10 mM Tris-HCl pH 8, 0.1 mM EDTA).

    Article Title: Dynamics of genome reorganization during human cardiogenesis reveal an RBM20-dependent splicing factory
    Article Snippet: .. DNA was amplified using NEBNext High-Fidelity 2× Master Mix (M0541L) and quantified with a Qubit. .. DNA was amplified using NEBNext High-Fidelity 2× Master Mix (M0541L) and quantified with a Qubit.

    Article Title: RELACS nuclei barcoding enables high-throughput ChIP-seq
    Article Snippet: .. Library amplification for RELACS ChIP-seq samples 18 µL of RELACS ChIP DNA was mixed together with 25 µL of PCR master mix from NEBNext Ultra II DNA library preparation (E7645L, NEB), 3 µL of USER enzyme and 2 µL of 10 μM universal PCR primer. .. Hairpin adapters were opened by a 15 min incubation at 37 °C.

    Article Title: Guidance of regulatory T cell development by Satb1-dependent super-enhancer establishment
    Article Snippet: .. Purified DNA was amplified using NEBNext High-Fidelity PCR Master Mix (New England BioLabs) with the following primers: forward 5′-TCGTCGGCAGCGTCAGATGTG-3′ and reverse 5′-GTCTCGTGGGCTCGGAGATGT-3′. .. PCR fragments were size-selected (150–500 bp) with Agencourt AMPure XP (Beckman Coulter), sonicated by Covaris Focused-ultrasonicator S220, subjected to library preparation with KAPA Library Preparation Kit, and sequenced by Ion Proton.

    Purification:

    Article Title: Guidance of regulatory T cell development by Satb1-dependent super-enhancer establishment
    Article Snippet: .. Purified DNA was amplified using NEBNext High-Fidelity PCR Master Mix (New England BioLabs) with the following primers: forward 5′-TCGTCGGCAGCGTCAGATGTG-3′ and reverse 5′-GTCTCGTGGGCTCGGAGATGT-3′. .. PCR fragments were size-selected (150–500 bp) with Agencourt AMPure XP (Beckman Coulter), sonicated by Covaris Focused-ultrasonicator S220, subjected to library preparation with KAPA Library Preparation Kit, and sequenced by Ion Proton.

    Real-time Polymerase Chain Reaction:

    Article Title: Transcriptome-wide identification of novel circular RNAs in soybean in response to low-phosphorus stress
    Article Snippet: .. Quantitative polymerase chain reaction (qPCR) was subsequently conducted in conjunction with universal PCR primers, NEBNext High-Fidelity PCR Master Mix, and Index (X) primer. .. To assess the library quality, an Agilent Bioanalyzer 2100 system was used to purify the PCR products (AMPure XP system).

    Polymerase Chain Reaction:

    Article Title: Efficient Genome-Wide Sequencing and Low-Coverage Pedigree Analysis from Noninvasively Collected Samples
    Article Snippet: .. A final PCR was carried out in a 50-μl reaction volume, using 23 μl of the posthybridization fDNA and either (1) 25 μl 2× KAPA High Fidelity master mix and 2 μl TruSeq universal primer (capture 1) or (2) 25 μl 2× NEBNext High Fidelity PCR master mix, 1 μl universal PCR primer, and 1 μl NEB indexing primer (capture 2). .. After 12 PCR cycles the final reaction was purified with 1× AMPure beads, eluted in 20 μL H2O, and visualized on a Bioanalyzer High Sensitivity DNA chip.

    Article Title: Cooperative interactions enable singular olfactory receptor expression in mouse olfactory neurons
    Article Snippet: .. The transposed DNA was then amplified using barcoded primers and NEBNext High Fidelity 2x PCR Master Mix (NEB). .. Amplified libraries were purified using Ampure XP beads (Beckman Coulter) at a ratio of 1.6 ul of beads per 1 uL of library and eluted in 30 uL of elution buffer (10 mM Tris-HCl pH 8, 0.1 mM EDTA).

    Article Title: Quartz-Seq2: a high-throughput single-cell RNA-sequencing method that effectively uses limited sequence reads
    Article Snippet: .. In addition, we used SuperScript III instead of ProtoScript in the RT step and KAPA HiFi DNA polymerase instead of NEBNext High-Fidelity PCR DNA polymerase in the PCR step. .. The resulting sequence library DNA was analyzed by HiSeq2500.

    Article Title: Transcriptome-wide identification of novel circular RNAs in soybean in response to low-phosphorus stress
    Article Snippet: .. Quantitative polymerase chain reaction (qPCR) was subsequently conducted in conjunction with universal PCR primers, NEBNext High-Fidelity PCR Master Mix, and Index (X) primer. .. To assess the library quality, an Agilent Bioanalyzer 2100 system was used to purify the PCR products (AMPure XP system).

    Article Title: RELACS nuclei barcoding enables high-throughput ChIP-seq
    Article Snippet: .. Library amplification for RELACS ChIP-seq samples 18 µL of RELACS ChIP DNA was mixed together with 25 µL of PCR master mix from NEBNext Ultra II DNA library preparation (E7645L, NEB), 3 µL of USER enzyme and 2 µL of 10 μM universal PCR primer. .. Hairpin adapters were opened by a 15 min incubation at 37 °C.

    Article Title: Guidance of regulatory T cell development by Satb1-dependent super-enhancer establishment
    Article Snippet: .. Purified DNA was amplified using NEBNext High-Fidelity PCR Master Mix (New England BioLabs) with the following primers: forward 5′-TCGTCGGCAGCGTCAGATGTG-3′ and reverse 5′-GTCTCGTGGGCTCGGAGATGT-3′. .. PCR fragments were size-selected (150–500 bp) with Agencourt AMPure XP (Beckman Coulter), sonicated by Covaris Focused-ultrasonicator S220, subjected to library preparation with KAPA Library Preparation Kit, and sequenced by Ion Proton.

    Article Title: Mapping RNA-chromatin interactions by sequencing with iMARGI
    Article Snippet: .. We recommend optimizing the PCR cycle number by setting up a 50 μL test PCR reaction mix using 5 μL of linearized DNA, 25 μL of 2× NEBNext HC PCR Master Mix, 1 μL of Universal Primer (10 μM), 1 μL of Index Primer (10 μM) and 18 μL of H2O. .. Aliquot the PCR reaction mix into five 10 μL reaction for PCR cycle screening.

    Chromatin Immunoprecipitation:

    Article Title: RELACS nuclei barcoding enables high-throughput ChIP-seq
    Article Snippet: .. Library amplification for RELACS ChIP-seq samples 18 µL of RELACS ChIP DNA was mixed together with 25 µL of PCR master mix from NEBNext Ultra II DNA library preparation (E7645L, NEB), 3 µL of USER enzyme and 2 µL of 10 μM universal PCR primer. .. Hairpin adapters were opened by a 15 min incubation at 37 °C.

    ChIP-sequencing:

    Article Title: RELACS nuclei barcoding enables high-throughput ChIP-seq
    Article Snippet: .. Library amplification for RELACS ChIP-seq samples 18 µL of RELACS ChIP DNA was mixed together with 25 µL of PCR master mix from NEBNext Ultra II DNA library preparation (E7645L, NEB), 3 µL of USER enzyme and 2 µL of 10 μM universal PCR primer. .. Hairpin adapters were opened by a 15 min incubation at 37 °C.

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  • 99
    New England Biolabs q5 hot star high fidelity dna polymerase
    Performance of four thermostable <t>DNA</t> polymerases for the synthesis of gene B using PCA-DTF. Lane 1: negative control reaction performed at the same conditions, without addition of primers; lane 2: KOD Hot Start DNA polymerase; lane 3: <t>Q5</t> Hot Start High Fidelity DNA polymerase; lane 4: Pfu Turbo DNA polymerase and lane 5: Taq DNA polymerase. M2: NZYladder I
    Q5 Hot Star High Fidelity Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/q5 hot star high fidelity dna polymerase/product/New England Biolabs
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    q5 hot star high fidelity dna polymerase - by Bioz Stars, 2021-02
    99/100 stars
      Buy from Supplier

    99
    New England Biolabs q5 dna polymerase
    Optimization of EvaGreen- and <t>Q5</t> DNA polymerase-based dNTP quantification. ( A-F ) Each critical reaction component and reaction temperature were varied and the outcome evaluated. ( G ) Assay conditions in graphs A to F . The standard curves were generated from end-point (1h) baseline-corrected fluorescence values.
    Q5 Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1562 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/q5 dna polymerase/product/New England Biolabs
    Average 99 stars, based on 1562 article reviews
    Price from $9.99 to $1999.99
    q5 dna polymerase - by Bioz Stars, 2021-02
    99/100 stars
      Buy from Supplier

    Image Search Results


    Performance of four thermostable DNA polymerases for the synthesis of gene B using PCA-DTF. Lane 1: negative control reaction performed at the same conditions, without addition of primers; lane 2: KOD Hot Start DNA polymerase; lane 3: Q5 Hot Start High Fidelity DNA polymerase; lane 4: Pfu Turbo DNA polymerase and lane 5: Taq DNA polymerase. M2: NZYladder I

    Journal: BMC Biotechnology

    Article Title: Development of a gene synthesis platform for the efficient large scale production of small genes encoding animal toxins

    doi: 10.1186/s12896-016-0316-3

    Figure Lengend Snippet: Performance of four thermostable DNA polymerases for the synthesis of gene B using PCA-DTF. Lane 1: negative control reaction performed at the same conditions, without addition of primers; lane 2: KOD Hot Start DNA polymerase; lane 3: Q5 Hot Start High Fidelity DNA polymerase; lane 4: Pfu Turbo DNA polymerase and lane 5: Taq DNA polymerase. M2: NZYladder I

    Article Snippet: Finally, extension was performed at 70 °C for 3 s for KOD Hot Start DNA polymerase, 72 °C for 30 s for Pfu Turbo and Taq, and 72 °C for 15 s for Q5® Hot Star High Fidelity DNA polymerase.

    Techniques: Negative Control

    Optimization of EvaGreen- and Q5 DNA polymerase-based dNTP quantification. ( A-F ) Each critical reaction component and reaction temperature were varied and the outcome evaluated. ( G ) Assay conditions in graphs A to F . The standard curves were generated from end-point (1h) baseline-corrected fluorescence values.

    Journal: bioRxiv

    Article Title: A sensitive assay for dNTPs based on long synthetic oligonucleotides, EvaGreen dye, and inhibitor-resistant high-fidelity DNA polymerase

    doi: 10.1101/2019.12.17.879122

    Figure Lengend Snippet: Optimization of EvaGreen- and Q5 DNA polymerase-based dNTP quantification. ( A-F ) Each critical reaction component and reaction temperature were varied and the outcome evaluated. ( G ) Assay conditions in graphs A to F . The standard curves were generated from end-point (1h) baseline-corrected fluorescence values.

    Article Snippet: Based on the lowest background signal, and reported ( ) inhibitor resistance in DNA extraction-free PCR, we chose to continue the optimization with Q5 DNA polymerase.

    Techniques: Generated, Fluorescence

    Long synthetic DNA oligonucleotides, an inhibitor-resistant high-fidelity DNA polymerase and EvaGreen detection chemistry allow quantification of dNTPs from mouse liver extracts. ( A ) Representative quantification of dTTP using the published fluorometric probe-hydrolysis-based assay. For clarity, curves from three lowest standard samples (0.31 to 0.08 pmol) are not shown. ( B ) Representative quantification of dTTP using Q5 DNA polymerase, long oligonucleotide template and EvaGreen detection chemistry. ( C - D ) dTTP signal generated by the fluorometric methods from mouse liver extracts with and without 0.5 pmol dTTP spike-in calibrant. The final extract volume was diluted to 80 µl per 40 mg of initial tissue weight. ( E - F ) Standard curves generated from the end-point baseline-corrected fluorescence values. The lowest y-axis value shows the background signal. Gray lines present the 95% confidence interval of the non-linear curve fit.

    Journal: bioRxiv

    Article Title: A sensitive assay for dNTPs based on long synthetic oligonucleotides, EvaGreen dye, and inhibitor-resistant high-fidelity DNA polymerase

    doi: 10.1101/2019.12.17.879122

    Figure Lengend Snippet: Long synthetic DNA oligonucleotides, an inhibitor-resistant high-fidelity DNA polymerase and EvaGreen detection chemistry allow quantification of dNTPs from mouse liver extracts. ( A ) Representative quantification of dTTP using the published fluorometric probe-hydrolysis-based assay. For clarity, curves from three lowest standard samples (0.31 to 0.08 pmol) are not shown. ( B ) Representative quantification of dTTP using Q5 DNA polymerase, long oligonucleotide template and EvaGreen detection chemistry. ( C - D ) dTTP signal generated by the fluorometric methods from mouse liver extracts with and without 0.5 pmol dTTP spike-in calibrant. The final extract volume was diluted to 80 µl per 40 mg of initial tissue weight. ( E - F ) Standard curves generated from the end-point baseline-corrected fluorescence values. The lowest y-axis value shows the background signal. Gray lines present the 95% confidence interval of the non-linear curve fit.

    Article Snippet: Based on the lowest background signal, and reported ( ) inhibitor resistance in DNA extraction-free PCR, we chose to continue the optimization with Q5 DNA polymerase.

    Techniques: Hydrolysis Assay, Generated, Fluorescence

    Assay specificity for PIK 3 CA mutations. Comparison of mutant PIK 3 CA detection in human control genomic DNA ( gDNA ) and cell‐free plasma DNA following pre‐amplification or no pre‐amplification using Q5 High‐Fidelity DNA Polymerase (New England BioLabs) or TaqMan ® PreAmp Master Mix (Life Technologies). The level of blank (LoB) was 0.04% for the mutation E545K and for H1047R, 0.02% for E542K, and less than 0.01% for H1047L as shown by the graphs.

    Journal: Molecular Oncology

    Article Title: Correlation between circulating cell‐free PIK3CA tumor DNA levels and treatment response in patients with PIK3CA‐mutated metastatic breast cancer

    doi: 10.1002/1878-0261.12305

    Figure Lengend Snippet: Assay specificity for PIK 3 CA mutations. Comparison of mutant PIK 3 CA detection in human control genomic DNA ( gDNA ) and cell‐free plasma DNA following pre‐amplification or no pre‐amplification using Q5 High‐Fidelity DNA Polymerase (New England BioLabs) or TaqMan ® PreAmp Master Mix (Life Technologies). The level of blank (LoB) was 0.04% for the mutation E545K and for H1047R, 0.02% for E542K, and less than 0.01% for H1047L as shown by the graphs.

    Article Snippet: To increase the sensitivity of the analysis, the remaining 75 μL serum DNA was subjected to 12 cycles of PCR pre‐amplification with Q5 High‐Fidelity DNA Polymerase (New England BioLabs, Ipswich, MA, USA) using a multiplex PIK3CA primer mix.

    Techniques: Mutagenesis, Amplification