phusion (New England Biolabs)
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New England Biolabs
phusion
Phusion, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 166 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/phusion/product/New England Biolabs
Average 99 stars, based on 166 article reviews
Price from $9.99 to $1999.99
Phusion, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 166 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/phusion/product/New England Biolabs
Average 99 stars, based on 166 article reviews
Price from $9.99 to $1999.99
phusion - by Bioz Stars,
2020-03
99/100 stars
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1) Product Images from "In vitro synthesis of gene-length single-stranded DNA"
Article Title: In vitro synthesis of gene-length single-stranded DNA
Journal: Scientific Reports
doi: 10.1038/s41598-018-24677-5
Figure Legend Snippet: ssDNA production by aPCR. ( a ) aPCR reactions were assembled with a 50-molar excess of a forward primer for the amplification of a 1,000 nt ssDNA fragment using the M13mp18 ssDNA plasmid as template, and with 10 different polymerases that were tested for highest yield of ssDNA production (upper band: expected dsDNA size is 1,000 bp; lower band: expected ssDNA size is 1,000 nt) as judged by agarose gel electrophoresis (right panel). QuantaBio AccuStart HiFi, polymerase (lane 2, boxed) produced the highest amount without overlapping dsDNA contaminants. 1. Accustart; 2. Accustart HiFi; 3. Accustart II; 4. AccuPrime; 5. GoTaq; 6. DreamTaq; 7. Phusion; 8. Platinum SuperFi; 9. Q5; 10. Tth polymerase. ( b ) Biochemical validation of ssDNA production by incubating 1,000 nt aPCR reaction products with the ssDNA-specific ExoI or S1 nucleases or dsDNA-specific restriction enzymes Eco RI and Nae I (left panel). Agarose gel electrophoresis of the digestion products as labeled by lane (right panel). M: Marker, C: aPCR product control, ExoI: exonuclease I, S1: S1 nuclease, Enz: Eco RI + Nae I. ( c ) NEB LongAmp was used to generate ssDNA up to 15,000 nt long using lambda phage dsDNA as template. Purification of the 10 kb fragment shows a single band of higher molecular weight than the M13mp18 ssDNA (7,249 nt). ( d ) The primer design algorithm aPrime was used to select primers for product sizes between 500 and 3,000 nt using M13mp18 ssDNA as template and the Quantabio Accustart HiFi enzyme. SYBR Safe stained agarose gels illuminated under blue light show dsDNA as yellow bands, while ssDNA show as orange bands.
Techniques Used: Amplification, Plasmid Preparation, Agarose Gel Electrophoresis, Produced, Labeling, Marker, Purification, Molecular Weight, Staining
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Southern Blot:Article Title: Glycerol-Mediated Repression of Glucose Metabolism and Glycerol Kinase as the Sole Route of Glycerol Catabolism in the Haloarchaeon Haloferax volcanii ▿ ▿ † Article Snippet: Infection:Article Title: Aminoacyl-tRNA-Charged Eukaryotic Elongation Factor 1A Is the Bona Fide Substrate for Legionella pneumophila Effector Glucosyltransferases Article Snippet: Murine RAW 264.7 macrophages were used for infection studies. .. PfuII Turbo DNA Polymerase was from Stratagene and Digital PCR:Article Title: Detection fidelity of AR mutations in plasma derived cell-free DNA Article Snippet: Paragraph title: Droplet digital PCR ... 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All PCRs were performed using Molecular Cloning:Article Title: Recognition motif and mechanism of ripening inhibitory peptides in plant hormone receptor ETR1 Article Snippet: Paragraph title: Molecular cloning ... Each fragment was amplified in a PCR with In Vivo:Article Title: CATP-6, a C. elegans Ortholog of ATP13A2 PARK9, Positively Regulates GEM-1, an SLC16A Transporter Article Snippet: For large PCR products we used either Magnetic Beads:Article Title: Accelerated Chromatin Biochemistry Using DNA-Barcoded Nucleosome Libraries Article Snippet: Sf9 cells, MyOne streptavidin magnetic beads, SybrGold, Qubit high sensitivity ds DNA quantification kit, and ethidium bromide DNA stain were purchased from Invitrogen. .. T4 DNA ligase, T4 Polynucleotide Kinase, Mutagenesis:Article Title: Heterologous Biosynthesis, Modifications and Structural Characterization of Ruminococcin-A, a Lanthipeptide From the Gut Bacterium Ruminococcus gnavus E1, in Escherichia coli Article Snippet: Paragraph title: PCR Cloning, Mutagenesis, and Construction of Expression Vectors ... Article Title: Recognition motif and mechanism of ripening inhibitory peptides in plant hormone receptor ETR1 Article Snippet: In short, the mutagenesis PCR primers were designed in either PCRdesign or AAscan program with a 21-nucleotides overlap for a mutagenesis primer pair. .. Each fragment was amplified in a PCR with Article Title: Accelerated Chromatin Biochemistry Using DNA-Barcoded Nucleosome Libraries Article Snippet: T4 DNA ligase, T4 Polynucleotide Kinase, Isolation:Article Title: Heterologous Biosynthesis, Modifications and Structural Characterization of Ruminococcin-A, a Lanthipeptide From the Gut Bacterium Ruminococcus gnavus E1, in Escherichia coli Article Snippet: Purification:Article Title: The Bacteriophage EF-P29 Efficiently Protects against Lethal Vancomycin-Resistant Enterococcus faecalis and Alleviates Gut Microbiota Imbalance in a Murine Bacteremia Model Article Snippet: All PCRs were performed using Polymerase Chain Reaction:Article Title: Heterologous Biosynthesis, Modifications and Structural Characterization of Ruminococcin-A, a Lanthipeptide From the Gut Bacterium Ruminococcus gnavus E1, in Escherichia coli Article Snippet: Paragraph title: PCR Cloning, Mutagenesis, and Construction of Expression Vectors ... Article Title: Mammalian cells lacking either the cotranslational or posttranslocational oligosaccharyltransferase complex display substrate-dependent defects in asparagine linked glycosylation Article Snippet: Primers for PCR amplification of genomic DNA flanking the CRISPR target sites were designed using PRIMER BLAST (NCBI). .. Genomic fragments were amplified with Article Title: The Bacteriophage EF-P29 Efficiently Protects against Lethal Vancomycin-Resistant Enterococcus faecalis and Alleviates Gut Microbiota Imbalance in a Murine Bacteremia Model Article Snippet: .. All PCRs were performed using Article Title: CATP-6, a C. elegans Ortholog of ATP13A2 PARK9, Positively Regulates GEM-1, an SLC16A Transporter Article Snippet: .. For large PCR products we used either Article Title: Generation, expression and utilization of single-domain antibodies for in vivo protein localization and manipulation in sea urchin embryos Article Snippet: PCR amplification is routinely used to subclone the Nb sequence into the expression vector; Nbs range from 350 to 500 bp in size. .. We routinely use Article Title: Recognition motif and mechanism of ripening inhibitory peptides in plant hormone receptor ETR1 Article Snippet: .. Each fragment was amplified in a PCR with Article Title: Lipoate-binding proteins and specific lipoate-protein ligases in microbial sulfur oxidation reveal an atpyical role for an old cofactor Article Snippet: .. PCR amplification was performed using Taq, Article Title: Sorbin and SH3 domain-containing protein 2 (SORBS2) is a component of the acto-myosin ring at the apical junctional complex in epithelial cells Article Snippet: Paragraph title: SORBS1, 2 and 3 qRT-PCR and SORBS2 PCR for isoform identification ... Primers were designed that could identify all, or specific isoforms, of SORBS2 in canine- or human cells (SORBS2 primers, ) and DNA was amplified ( Article Title: Detection fidelity of AR mutations in plasma derived cell-free DNA Article Snippet: .. Genomic DNA, patient cfDNA, and water (no template control) were PCR amplified using Quantitative RT-PCR:Article Title: Sorbin and SH3 domain-containing protein 2 (SORBS2) is a component of the acto-myosin ring at the apical junctional complex in epithelial cells Article Snippet: Paragraph title: SORBS1, 2 and 3 qRT-PCR and SORBS2 PCR for isoform identification ... Primers were designed that could identify all, or specific isoforms, of SORBS2 in canine- or human cells (SORBS2 primers, ) and DNA was amplified ( CRISPR:Article Title: Mammalian cells lacking either the cotranslational or posttranslocational oligosaccharyltransferase complex display substrate-dependent defects in asparagine linked glycosylation Article Snippet: Primers for PCR amplification of genomic DNA flanking the CRISPR target sites were designed using PRIMER BLAST (NCBI). .. Genomic fragments were amplified with cDNA Library Assay:Article Title: Generation, expression and utilization of single-domain antibodies for in vivo protein localization and manipulation in sea urchin embryos Article Snippet: Depending on your Nb target, template may come from a cDNA library of screened Nbs, or from commercially or publicly available plasmid sources. .. We routinely use Agarose Gel Electrophoresis:Article Title: Mammalian cells lacking either the cotranslational or posttranslocational oligosaccharyltransferase complex display substrate-dependent defects in asparagine linked glycosylation Article Snippet: Genomic fragments were amplified with Article Title: Generation, expression and utilization of single-domain antibodies for in vivo protein localization and manipulation in sea urchin embryos Article Snippet: We routinely use Article Title: Sorbin and SH3 domain-containing protein 2 (SORBS2) is a component of the acto-myosin ring at the apical junctional complex in epithelial cells Article Snippet: Primers were designed that could identify all, or specific isoforms, of SORBS2 in canine- or human cells (SORBS2 primers, ) and DNA was amplified ( Polymerase Cycling Assembly:Article Title: Cysteine Toxicity Drives Age-Related Mitochondrial Decline by Altering Iron Homeostasis. Article Snippet: Mitochondria and lysosomes are functionally linked, and their interdependent Plasmid Preparation:Article Title: Aminoacyl-tRNA-Charged Eukaryotic Elongation Factor 1A Is the Bona Fide Substrate for Legionella pneumophila Effector Glucosyltransferases Article Snippet: Expression vector pGEX-4T was from GE Healthcare (Freiburg, Germany), pET28a vector from Novagen (Madison, WI). pBC KS (+) and pBluescript KS (+) vectors were from Stratagene (Waldbronn, Germany). .. PfuII Turbo DNA Polymerase was from Stratagene and Article Title: Heterologous Biosynthesis, Modifications and Structural Characterization of Ruminococcin-A, a Lanthipeptide From the Gut Bacterium Ruminococcus gnavus E1, in Escherichia coli Article Snippet: Article Title: Mammalian cells lacking either the cotranslational or posttranslocational oligosaccharyltransferase complex display substrate-dependent defects in asparagine linked glycosylation Article Snippet: Genomic fragments were amplified with Article Title: Cysteine Toxicity Drives Age-Related Mitochondrial Decline by Altering Iron Homeostasis. Article Snippet: .. Mitochondria and lysosomes are functionally linked, and their interdependent Article Title: Generation, expression and utilization of single-domain antibodies for in vivo protein localization and manipulation in sea urchin embryos Article Snippet: PCR amplification is routinely used to subclone the Nb sequence into the expression vector; Nbs range from 350 to 500 bp in size. .. We routinely use Article Title: Recognition motif and mechanism of ripening inhibitory peptides in plant hormone receptor ETR1 Article Snippet: The new constructs were made by using a two-fragment PCR approach starting from the expression plasmid pTEV-16b-AtETR1 that contains the full-length Arabidopsis thaliana ethylene receptor 1 (AtETR1) cDNA. .. Each fragment was amplified in a PCR with Article Title: Seamless assembly of recombinant adenoviral genomes from high-copy plasmids Article Snippet: Paragraph title: Plasmid construction ... Amplification was performed with Software:Article Title: Mammalian cells lacking either the cotranslational or posttranslocational oligosaccharyltransferase complex display substrate-dependent defects in asparagine linked glycosylation Article Snippet: Genomic fragments were amplified with Positron Emission Tomography:Article Title: Recognition motif and mechanism of ripening inhibitory peptides in plant hormone receptor ETR1 Article Snippet: All truncated AtETR1 constructs and AtETR11–307 mutants were prepared in pTEV-16b vector backbone , a modified version of pET-16b (Novagen, Darmstadt, Germany) containing the N-terminal decahistidine-tag followed by a linker (SSGH) and a tobacco etch virus (TEV) protease cleavage site (ENLYFQG; instead of a Factor Xa cleavage site in pET-16b). .. Each fragment was amplified in a PCR with Sample Prep:Article Title: The Bacteriophage EF-P29 Efficiently Protects against Lethal Vancomycin-Resistant Enterococcus faecalis and Alleviates Gut Microbiota Imbalance in a Murine Bacteremia Model Article Snippet: All PCRs were performed using Strep-tag:Article Title: Lipoate-binding proteins and specific lipoate-protein ligases in microbial sulfur oxidation reveal an atpyical role for an old cofactor Article Snippet: PCR amplification was performed using Taq, Staining:Article Title: Accelerated Chromatin Biochemistry Using DNA-Barcoded Nucleosome Libraries Article Snippet: Sf9 cells, MyOne streptavidin magnetic beads, SybrGold, Qubit high sensitivity ds DNA quantification kit, and ethidium bromide DNA stain were purchased from Invitrogen. .. T4 DNA ligase, T4 Polynucleotide Kinase, |
