p9310 (New England Biolabs)
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Name:
Anti SNAP tag Antibody Polyclonal
Description:
Anti SNAP tag Antibody Polyclonal 100 ul
Catalog Number:
p9310s
Price:
270
Size:
100 ul
Category:
Primary Antibodies
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Structured Review
New England Biolabs
p9310
Anti SNAP tag Antibody Polyclonal 100 ul
https://www.bioz.com/result/p9310/product/New England Biolabs
Average 99 stars, based on 2 article reviews
Price from $9.99 to $1999.99
Anti SNAP tag Antibody Polyclonal 100 ul
https://www.bioz.com/result/p9310/product/New England Biolabs
Average 99 stars, based on 2 article reviews
Price from $9.99 to $1999.99
p9310 - by Bioz Stars,
2020-03
99/100 stars
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Transduction:Article Title: The anti-tumor drug 2-hydroxyoleic acid (Minerval) stimulates signaling and retrograde transport Article Snippet: .. The following antibodies were used: phospho-AKT1Ser473 (#9271), AKT1 (#4691), phospho-RPS6KBThr389 (#9205), RPS6KB (#9202), phospho-Erk/ MAPK1/3Thr202/Tyr204 (#9106), Erk/MAPK1/3 (#9102), phospho-p38/MAPK14Thr180/Tyr182 (#9211), phospho-JUN (#9164), ERBB2Tyr877 (#2241), PERK /EIF2AK3 (#5683) all from Cell Signaling Technology, and p-38/MAPK14 (#612168, Transduction Laboratories), GFP (clone 3H9, ChromoTek), phospho-EGFRTyr1173 (#ab24912, Abcam), GAPDH (#ab9482, Abcam), ACTIN (#CLT9001, Cedarlane), Luciferase:Article Title: Corticotropin releasing hormone receptor 2 exacerbates chronic cardiac dysfunction Article Snippet: .. Incubation:Article Title: The anti-tumor drug 2-hydroxyoleic acid (Minerval) stimulates signaling and retrograde transport Article Snippet: The membrane was blocked in 7.5% milk for 45 minutes, followed by overnight incubation with the indicated antibodies in 5% BSA, 45 minutes incubation with HRP-conjugated secondary antibodies and detection with SuperSignal West Dura Extended Duration Substrate (Thermo Scientific) in a ChemiDoc Imaging System (Bio-Rad). .. The following antibodies were used: phospho-AKT1Ser473 (#9271), AKT1 (#4691), phospho-RPS6KBThr389 (#9205), RPS6KB (#9202), phospho-Erk/ MAPK1/3Thr202/Tyr204 (#9106), Erk/MAPK1/3 (#9102), phospho-p38/MAPK14Thr180/Tyr182 (#9211), phospho-JUN (#9164), ERBB2Tyr877 (#2241), PERK /EIF2AK3 (#5683) all from Cell Signaling Technology, and p-38/MAPK14 (#612168, Transduction Laboratories), GFP (clone 3H9, ChromoTek), phospho-EGFRTyr1173 (#ab24912, Abcam), GAPDH (#ab9482, Abcam), ACTIN (#CLT9001, Cedarlane), Article Title: Heparan sulfate antagonism alters bone morphogenetic protein signaling and receptor dynamics, suggesting a mechanism in hereditary multiple exostoses Article Snippet: .. Membranes were blocked in TBST and incubated overnight at 4 °C with Article Title: Fluorescence Correlation Spectroscopy Reveals Efficient Cytosolic Delivery of Protein Cargo by Cell-Permeant Miniature Proteins Article Snippet: .. SNAP-tag was detected by incubating the membrane with an Article Title: Hepatitis B virus PreS2-mutant large surface antigen activates store-operated calcium entry and promotes chromosome instability Article Snippet: The slides were then incubated with mouse anti-preS1 for 1 hr and assessed using the EnVision+ System-HRP kit (DAKO, Carpintena, CA, USA). .. The following primary antibodies were used in this study: mouse anti-preS1 (a gift from Ningshao Xia, Xiamen University, China), Article Title: The novel function of tumor protein D54 in regulating pyruvate dehydrogenase and metformin cytotoxicity in breast cancer Article Snippet: Following this incubation, membranes were washed in 1× TBST for 30 min, followed by incubation with secondary antibody for an additional hour. .. The antibodies used were as follows: TPD54 (Abcam, AB194938), Pyruvate dehydrogenase (PDH) WB antibody cocktail (Abcam, ab110416), NDUFB8 antibody [20E9DH10C12] (Abcam, AB110242), PDH E1α (Abcam, ab110334), PDH E1α (Proteintech, 18068-1-AP), PDH E1α (Proteintech, 66,119-1-Ig), PhosphoS232 PDH E1α (LSBio, LS-C265964), PhosphoS293 PDH E1α (Novus, NB110-93479), phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb (Cell signaling, Cat#9718), vinculin (Cell signaling, Cat#4650), Actin (Cell signaling, cat# 3700), Cleaved PARP (Cell signaling, Cat#5625), ubiquitin (Abcam, ab7780), PDK1 (Santa Cruz,sc36203), PDK2 (Santa Cruz, sc517284), Expressing:Article Title: Regulating G protein-coupled receptors by topological inversion Article Snippet: Materials We obtained anti-human CCR5 45531 from R and D Systems (Minneapolis, MN), anti-actin from Sigma Aldrich (St. Louis, MO), anti-Giantin 924302 from Biolegend (San Diego, CA), Alexa Fluor 488 FluoroNanogold goat anti-mouse IgG Fab from Nanoprobes.com (Yaphank, NY), AffiniPure Donkey Anti-Rabbit IgG (H + L) from Jackson ImmunoResearch (West Grove, PA), Alexa Fluor 488 goat Anti-Mouse IgG (H + L) from Invitrogen (Carlsbad, CA), Western Blot:Article Title: Agonist-induced membrane nanodomain clustering drives GLP-1 receptor responses in pancreatic beta cells Article Snippet: .. Antibodies for western blotting were anti-flotillin-1 mouse monoclonal antibody, catalogue no. sc-74566, Santa Cruz Biotechnology (1:200 dilution); anti-flotillin-1 rat monoclonal antibody, catalogue no. 849802, BioLegend (1:100 dilution); anti-SNAP-tag rabbit polyclonal antibody, catalogue no. Article Title: Fluorescence Correlation Spectroscopy Reveals Efficient Cytosolic Delivery of Protein Cargo by Cell-Permeant Miniature Proteins Article Snippet: The gel was analyzed by in-gel fluorescence scanning (Typhoon FLA 7000) prior to transfer onto PVDF membranes for Western blot analysis. .. SNAP-tag was detected by incubating the membrane with an Article Title: Molecular basis of signaling specificity between GIRK channels and GPCRs Article Snippet: .. Western Blot was performed using an Article Title: The novel function of tumor protein D54 in regulating pyruvate dehydrogenase and metformin cytotoxicity in breast cancer Article Snippet: .. The antibodies used were as follows: TPD54 (Abcam, AB194938), Pyruvate dehydrogenase (PDH) WB antibody cocktail (Abcam, ab110416), NDUFB8 antibody [20E9DH10C12] (Abcam, AB110242), PDH E1α (Abcam, ab110334), PDH E1α (Proteintech, 18068-1-AP), PDH E1α (Proteintech, 66,119-1-Ig), PhosphoS232 PDH E1α (LSBio, LS-C265964), PhosphoS293 PDH E1α (Novus, NB110-93479), phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb (Cell signaling, Cat#9718), vinculin (Cell signaling, Cat#4650), Actin (Cell signaling, cat# 3700), Cleaved PARP (Cell signaling, Cat#5625), ubiquitin (Abcam, ab7780), PDK1 (Santa Cruz,sc36203), PDK2 (Santa Cruz, sc517284), Modification:Article Title: RIP2 filament formation is required for NOD2 dependent NF-κB signalling Article Snippet: Immuno-gold labelling For immuno-gold labelling experiments, we modified protocol illustrated in Lu, A et al., 2015 , as follows. .. After removal of excess, grid was transferred to 30 µl drops of same solution containing either the rabbit antibody anti-SNAP at dilution 1:2000 ( Double Immunofluorescence Staining:Article Title: Hepatitis B virus PreS2-mutant large surface antigen activates store-operated calcium entry and promotes chromosome instability Article Snippet: Paragraph title: Immunohistochemical and double immunofluorescence staining ... The following primary antibodies were used in this study: mouse anti-preS1 (a gift from Ningshao Xia, Xiamen University, China), Electron Microscopy:Article Title: RIP2 filament formation is required for NOD2 dependent NF-κB signalling Article Snippet: For immuno-gold labelling, 6 µl of sample were applied to glow-discharged carbon coated copper grid (300 mesh, Electron Microscopy Science) and let adsorb for 30 s. Excess sample was blot off on filter paper, and grid was let shaking 10 min in 30 µl drop of PBS + 0.1% (w/v) BSA. .. After removal of excess, grid was transferred to 30 µl drops of same solution containing either the rabbit antibody anti-SNAP at dilution 1:2000 ( Transfection:Article Title: Molecular basis of signaling specificity between GIRK channels and GPCRs Article Snippet: Untransfected HEK-293T cells or HEK-293T cells transiently transfected with GPCR-GIRK4 concatemers were centrifuged and mixed with an equal volume of loading buffer containing 4% SDS and 10% β-mercaptoethanol. .. Western Blot was performed using an Protease Inhibitor:Article Title: The anti-tumor drug 2-hydroxyoleic acid (Minerval) stimulates signaling and retrograde transport Article Snippet: Immunoblotting Treated cells were washed with PBS and lysed (0.1 M NaCl, 10 mM Na2 HPO4 , pH 7.4, 1 mM EDTA, 1% Triton X-100, 60 mM n-octyl β-D-glucopyranoside, and cOmplete EDTA-free Protease Inhibitor Cocktail (Roche)). .. The following antibodies were used: phospho-AKT1Ser473 (#9271), AKT1 (#4691), phospho-RPS6KBThr389 (#9205), RPS6KB (#9202), phospho-Erk/ MAPK1/3Thr202/Tyr204 (#9106), Erk/MAPK1/3 (#9102), phospho-p38/MAPK14Thr180/Tyr182 (#9211), phospho-JUN (#9164), ERBB2Tyr877 (#2241), PERK /EIF2AK3 (#5683) all from Cell Signaling Technology, and p-38/MAPK14 (#612168, Transduction Laboratories), GFP (clone 3H9, ChromoTek), phospho-EGFRTyr1173 (#ab24912, Abcam), GAPDH (#ab9482, Abcam), ACTIN (#CLT9001, Cedarlane), Article Title: Fluorescence Correlation Spectroscopy Reveals Efficient Cytosolic Delivery of Protein Cargo by Cell-Permeant Miniature Proteins Article Snippet: This step was repeated to perform a second wash. After the second DPBS wash, the cells were resuspended in 1 mL of precooled buffered isotonic sucrose buffer (290 mM sucrose, 10 mM imidazole pH 7.0, 1 mM DTT, and 1 cOmplete protease inhibitor cocktail per 10 mL buffer) and repelleted at 500g for 2 min. Next, the cells were suspended in 150 μL of isotonic sucrose, transferred to 0.5 mL microtubes containing 1.4 mm ceramic beads (Omni International) and homogenized using a Bead Ruptor 4 (Omni International) at speed 1 for 8 s. The homogenized cells were transferred to polycarbonate ultracentrifuge tubes and centrifuged at 350 kg for 30 min at 4 °C to isolate the cytosolic fraction. .. SNAP-tag was detected by incubating the membrane with an other:Article Title: Unique Roles of β-Arrestin in GPCR Trafficking Revealed by Photoinducible Dimerizers Article Snippet: SNAP-Surface Alexa Fluor 647 and Article Title: Unique Roles of β-Arrestin in GPCR Trafficking Revealed by Photoinducible Dimerizers Article Snippet: Materials and cDNA Construction SNAP-Surface Alexa Fluor 647 and Imaging:Article Title: The anti-tumor drug 2-hydroxyoleic acid (Minerval) stimulates signaling and retrograde transport Article Snippet: The membrane was blocked in 7.5% milk for 45 minutes, followed by overnight incubation with the indicated antibodies in 5% BSA, 45 minutes incubation with HRP-conjugated secondary antibodies and detection with SuperSignal West Dura Extended Duration Substrate (Thermo Scientific) in a ChemiDoc Imaging System (Bio-Rad). .. The following antibodies were used: phospho-AKT1Ser473 (#9271), AKT1 (#4691), phospho-RPS6KBThr389 (#9205), RPS6KB (#9202), phospho-Erk/ MAPK1/3Thr202/Tyr204 (#9106), Erk/MAPK1/3 (#9102), phospho-p38/MAPK14Thr180/Tyr182 (#9211), phospho-JUN (#9164), ERBB2Tyr877 (#2241), PERK /EIF2AK3 (#5683) all from Cell Signaling Technology, and p-38/MAPK14 (#612168, Transduction Laboratories), GFP (clone 3H9, ChromoTek), phospho-EGFRTyr1173 (#ab24912, Abcam), GAPDH (#ab9482, Abcam), ACTIN (#CLT9001, Cedarlane), Article Title: The novel function of tumor protein D54 in regulating pyruvate dehydrogenase and metformin cytotoxicity in breast cancer Article Snippet: Proteins were detected by SuperSignal™ West Dura Extended Duration Substrate (Cat#34075) using the ChemiDoc™ Touch Imaging System. .. The antibodies used were as follows: TPD54 (Abcam, AB194938), Pyruvate dehydrogenase (PDH) WB antibody cocktail (Abcam, ab110416), NDUFB8 antibody [20E9DH10C12] (Abcam, AB110242), PDH E1α (Abcam, ab110334), PDH E1α (Proteintech, 18068-1-AP), PDH E1α (Proteintech, 66,119-1-Ig), PhosphoS232 PDH E1α (LSBio, LS-C265964), PhosphoS293 PDH E1α (Novus, NB110-93479), phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb (Cell signaling, Cat#9718), vinculin (Cell signaling, Cat#4650), Actin (Cell signaling, cat# 3700), Cleaved PARP (Cell signaling, Cat#5625), ubiquitin (Abcam, ab7780), PDK1 (Santa Cruz,sc36203), PDK2 (Santa Cruz, sc517284), Inverted Microscopy:Article Title: Hepatitis B virus PreS2-mutant large surface antigen activates store-operated calcium entry and promotes chromosome instability Article Snippet: The images were acquired with a Zeiss LSM780 confocal microscope or an automated Leica DMI6000 inverted microscope equipped with an HCX PL FL 20x/NA0.4 objective and an EMCCD camera (Andor Luca R, Belfast, UK) as indicated. .. The following primary antibodies were used in this study: mouse anti-preS1 (a gift from Ningshao Xia, Xiamen University, China), Recombinant:Article Title: Regulating G protein-coupled receptors by topological inversion Article Snippet: Materials We obtained anti-human CCR5 45531 from R and D Systems (Minneapolis, MN), anti-actin from Sigma Aldrich (St. Louis, MO), anti-Giantin 924302 from Biolegend (San Diego, CA), Alexa Fluor 488 FluoroNanogold goat anti-mouse IgG Fab from Nanoprobes.com (Yaphank, NY), AffiniPure Donkey Anti-Rabbit IgG (H + L) from Jackson ImmunoResearch (West Grove, PA), Alexa Fluor 488 goat Anti-Mouse IgG (H + L) from Invitrogen (Carlsbad, CA), Fluorescence:Article Title: Fluorescence Correlation Spectroscopy Reveals Efficient Cytosolic Delivery of Protein Cargo by Cell-Permeant Miniature Proteins Article Snippet: The gel was analyzed by in-gel fluorescence scanning (Typhoon FLA 7000) prior to transfer onto PVDF membranes for Western blot analysis. .. SNAP-tag was detected by incubating the membrane with an Mutagenesis:Article Title: Corticotropin releasing hormone receptor 2 exacerbates chronic cardiac dysfunction Article Snippet: Isolation:Article Title: Heparan sulfate antagonism alters bone morphogenetic protein signaling and receptor dynamics, suggesting a mechanism in hereditary multiple exostoses Article Snippet: Paragraph title: DRM isolation ... Membranes were blocked in TBST and incubated overnight at 4 °C with Immunohistochemistry:Article Title: Hepatitis B virus PreS2-mutant large surface antigen activates store-operated calcium entry and promotes chromosome instability Article Snippet: Paragraph title: Immunohistochemical and double immunofluorescence staining ... The following primary antibodies were used in this study: mouse anti-preS1 (a gift from Ningshao Xia, Xiamen University, China), Microscopy:Article Title: Hepatitis B virus PreS2-mutant large surface antigen activates store-operated calcium entry and promotes chromosome instability Article Snippet: The images were acquired with a Zeiss LSM780 confocal microscope or an automated Leica DMI6000 inverted microscope equipped with an HCX PL FL 20x/NA0.4 objective and an EMCCD camera (Andor Luca R, Belfast, UK) as indicated. .. The following primary antibodies were used in this study: mouse anti-preS1 (a gift from Ningshao Xia, Xiamen University, China), Purification:Article Title: RIP2 filament formation is required for NOD2 dependent NF-κB signalling Article Snippet: Immuno-gold labelling was applied after MBP and HIS-SUMO tags cleavage without further purification. .. After removal of excess, grid was transferred to 30 µl drops of same solution containing either the rabbit antibody anti-SNAP at dilution 1:2000 ( Labeling:Article Title: Clathrin promotes centrosome integrity in early mitosis through stabilization of centrosomal ch-TOG Article Snippet: Labeled proteins were detected using the ECL-Plus reagent (GE Healthcare). .. For detection of the SNAP-tag and CHC17 by blotting, the Chloramphenicol Acetyltransferase Assay:Article Title: The novel function of tumor protein D54 in regulating pyruvate dehydrogenase and metformin cytotoxicity in breast cancer Article Snippet: .. The antibodies used were as follows: TPD54 (Abcam, AB194938), Pyruvate dehydrogenase (PDH) WB antibody cocktail (Abcam, ab110416), NDUFB8 antibody [20E9DH10C12] (Abcam, AB110242), PDH E1α (Abcam, ab110334), PDH E1α (Proteintech, 18068-1-AP), PDH E1α (Proteintech, 66,119-1-Ig), PhosphoS232 PDH E1α (LSBio, LS-C265964), PhosphoS293 PDH E1α (Novus, NB110-93479), phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb (Cell signaling, Cat9718), vinculin (Cell signaling, Cat#4650), Actin (Cell signaling, cat# 3700), Cleaved PARP (Cell signaling, Cat#5625), ubiquitin (Abcam, ab7780), PDK1 (Santa Cruz,sc36203), PDK2 (Santa Cruz, sc517284), SDS Page:Article Title: Clathrin promotes centrosome integrity in early mitosis through stabilization of centrosomal ch-TOG Article Snippet: Immunoblot analysis Cell lysates were resolved by SDS-PAGE (NuPage Bis-Tris 4–12% SDS-acrylamide gels; Invitrogen), transferred to nitrocellulose membranes (EMD Millipore), and exposed to primary (1–5 µg/ml) and peroxidase-conjugated secondary antibodies. .. For detection of the SNAP-tag and CHC17 by blotting, the Article Title: The anti-tumor drug 2-hydroxyoleic acid (Minerval) stimulates signaling and retrograde transport Article Snippet: The cleared lysates were separated by 4–20% SDS-PAGE and transferred to a PVDF membrane. .. The following antibodies were used: phospho-AKT1Ser473 (#9271), AKT1 (#4691), phospho-RPS6KBThr389 (#9205), RPS6KB (#9202), phospho-Erk/ MAPK1/3Thr202/Tyr204 (#9106), Erk/MAPK1/3 (#9102), phospho-p38/MAPK14Thr180/Tyr182 (#9211), phospho-JUN (#9164), ERBB2Tyr877 (#2241), PERK /EIF2AK3 (#5683) all from Cell Signaling Technology, and p-38/MAPK14 (#612168, Transduction Laboratories), GFP (clone 3H9, ChromoTek), phospho-EGFRTyr1173 (#ab24912, Abcam), GAPDH (#ab9482, Abcam), ACTIN (#CLT9001, Cedarlane), Article Title: Agonist-induced membrane nanodomain clustering drives GLP-1 receptor responses in pancreatic beta cells Article Snippet: Paragraph title: SDS-PAGE and western blotting ... Antibodies for western blotting were anti-flotillin-1 mouse monoclonal antibody, catalogue no. sc-74566, Santa Cruz Biotechnology (1:200 dilution); anti-flotillin-1 rat monoclonal antibody, catalogue no. 849802, BioLegend (1:100 dilution); anti-SNAP-tag rabbit polyclonal antibody, catalogue no. Article Title: Fluorescence Correlation Spectroscopy Reveals Efficient Cytosolic Delivery of Protein Cargo by Cell-Permeant Miniature Proteins Article Snippet: Next, the cytosolic fractions were boiled in gel loading buffer and separated by SDS-PAGE. .. SNAP-tag was detected by incubating the membrane with an Article Title: Molecular basis of signaling specificity between GIRK channels and GPCRs Article Snippet: Samples were then run using standard SDS-PAGE procedures on Invitrogen NuPAGE 4–12% Bis-Tris gels and transferred onto PVDF membranes. .. Western Blot was performed using an Plasmid Preparation:Article Title: Corticotropin releasing hormone receptor 2 exacerbates chronic cardiac dysfunction Article Snippet: .. Software:Article Title: Clathrin promotes centrosome integrity in early mitosis through stabilization of centrosomal ch-TOG Article Snippet: Molecular mass markers (Rainbow; GE Healthcare) were used for calibration, and protein band intensities were quantified using Quantity One (Bio-Rad Laboratories) or ImageJ (version 1.45c) software. .. For detection of the SNAP-tag and CHC17 by blotting, the Article Title: The anti-tumor drug 2-hydroxyoleic acid (Minerval) stimulates signaling and retrograde transport Article Snippet: The signal intensities were quantified by the Quantity One software (Bio-Rad) and were normalized to the loading control. .. The following antibodies were used: phospho-AKT1Ser473 (#9271), AKT1 (#4691), phospho-RPS6KBThr389 (#9205), RPS6KB (#9202), phospho-Erk/ MAPK1/3Thr202/Tyr204 (#9106), Erk/MAPK1/3 (#9102), phospho-p38/MAPK14Thr180/Tyr182 (#9211), phospho-JUN (#9164), ERBB2Tyr877 (#2241), PERK /EIF2AK3 (#5683) all from Cell Signaling Technology, and p-38/MAPK14 (#612168, Transduction Laboratories), GFP (clone 3H9, ChromoTek), phospho-EGFRTyr1173 (#ab24912, Abcam), GAPDH (#ab9482, Abcam), ACTIN (#CLT9001, Cedarlane), Homogenization:Article Title: Heparan sulfate antagonism alters bone morphogenetic protein signaling and receptor dynamics, suggesting a mechanism in hereditary multiple exostoses Article Snippet: Following homogenization, 250 μl of 2% Triton X-100 was added to 250 μl of the cell homogenate and placed on ice for 30 min. For the step sucrose gradient, 1.25 ml of 56% sucrose was added to the sample for a final percentage of 40% sucrose. .. Membranes were blocked in TBST and incubated overnight at 4 °C with Activation Assay:Article Title: Corticotropin releasing hormone receptor 2 exacerbates chronic cardiac dysfunction Article Snippet: Fractionation:Article Title: Fluorescence Correlation Spectroscopy Reveals Efficient Cytosolic Delivery of Protein Cargo by Cell-Permeant Miniature Proteins Article Snippet: Paragraph title: Cytosolic Fractionation Assay ... SNAP-tag was detected by incubating the membrane with an Staining:Article Title: Hepatitis B virus PreS2-mutant large surface antigen activates store-operated calcium entry and promotes chromosome instability Article Snippet: For double immunofluorescence staining, the cells were fixed and stained with the indicated primary antibodies for 1 hr, followed by secondary antibodies conjugated with Alexa-488, Alexa-594, or Alexa-647 (Molecular Probes). .. The following primary antibodies were used in this study: mouse anti-preS1 (a gift from Ningshao Xia, Xiamen University, China), Article Title: RIP2 filament formation is required for NOD2 dependent NF-κB signalling Article Snippet: After removal of excess, grid was transferred to 30 µl drops of same solution containing either the rabbit antibody anti-SNAP at dilution 1:2000 ( |
