p9310  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    Anti SNAP tag Antibody Polyclonal
    Description:
    Anti SNAP tag Antibody Polyclonal 100 ul
    Catalog Number:
    p9310s
    Price:
    270
    Size:
    100 ul
    Category:
    Primary Antibodies
    Buy from Supplier


    Structured Review

    New England Biolabs p9310
    Anti SNAP tag Antibody Polyclonal
    Anti SNAP tag Antibody Polyclonal 100 ul
    https://www.bioz.com/result/p9310/product/New England Biolabs
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    p9310 - by Bioz Stars, 2020-03
    99/100 stars

    Related Products / Commonly Used Together

    rabbit antibody anti-snap
    ab197030
    rabbit anti-nod2

    Images

    Related Articles

    Transduction:

    Article Title: The anti-tumor drug 2-hydroxyoleic acid (Minerval) stimulates signaling and retrograde transport
    Article Snippet: .. The following antibodies were used: phospho-AKT1Ser473 (#9271), AKT1 (#4691), phospho-RPS6KBThr389 (#9205), RPS6KB (#9202), phospho-Erk/ MAPK1/3Thr202/Tyr204 (#9106), Erk/MAPK1/3 (#9102), phospho-p38/MAPK14Thr180/Tyr182 (#9211), phospho-JUN (#9164), ERBB2Tyr877 (#2241), PERK /EIF2AK3 (#5683) all from Cell Signaling Technology, and p-38/MAPK14 (#612168, Transduction Laboratories), GFP (clone 3H9, ChromoTek), phospho-EGFRTyr1173 (#ab24912, Abcam), GAPDH (#ab9482, Abcam), ACTIN (#CLT9001, Cedarlane), SNAP (#P9310S, New England Biolabs), Shiga toxin (#STX1-3C10, Toxin Technology), ricin (#C86370M, Meridian), and XBP1s (#647502, BioLegend). ..

    Luciferase:

    Article Title: Corticotropin releasing hormone receptor 2 exacerbates chronic cardiac dysfunction
    Article Snippet: .. Luciferase assay SNAP antibody (P9310S) and the pSNAPf plasmid were obtained from New England Biolabs. .. The vectors pGL4.29[luc2P/CRE/Hygro], pGL4.30[luc2P/NFAT-RE/Hygro], pGL4.33[luc2P/SRE/Hygro], and pGL4.34[luc2P/SRF-RE/Hygro] were purchased from Promega.

    Incubation:

    Article Title: The anti-tumor drug 2-hydroxyoleic acid (Minerval) stimulates signaling and retrograde transport
    Article Snippet: The membrane was blocked in 7.5% milk for 45 minutes, followed by overnight incubation with the indicated antibodies in 5% BSA, 45 minutes incubation with HRP-conjugated secondary antibodies and detection with SuperSignal West Dura Extended Duration Substrate (Thermo Scientific) in a ChemiDoc Imaging System (Bio-Rad). .. The following antibodies were used: phospho-AKT1Ser473 (#9271), AKT1 (#4691), phospho-RPS6KBThr389 (#9205), RPS6KB (#9202), phospho-Erk/ MAPK1/3Thr202/Tyr204 (#9106), Erk/MAPK1/3 (#9102), phospho-p38/MAPK14Thr180/Tyr182 (#9211), phospho-JUN (#9164), ERBB2Tyr877 (#2241), PERK /EIF2AK3 (#5683) all from Cell Signaling Technology, and p-38/MAPK14 (#612168, Transduction Laboratories), GFP (clone 3H9, ChromoTek), phospho-EGFRTyr1173 (#ab24912, Abcam), GAPDH (#ab9482, Abcam), ACTIN (#CLT9001, Cedarlane), SNAP (#P9310S, New England Biolabs), Shiga toxin (#STX1-3C10, Toxin Technology), ricin (#C86370M, Meridian), and XBP1s (#647502, BioLegend).

    Article Title: Heparan sulfate antagonism alters bone morphogenetic protein signaling and receptor dynamics, suggesting a mechanism in hereditary multiple exostoses
    Article Snippet: .. Membranes were blocked in TBST and incubated overnight at 4 °C with anti-SNAP-tag (1:1000; New England Biolabs) and caveolin-1 (1:1000; Cell Signaling). .. Membranes were washed in 1× TBST and incubated with anti-rabbit HRP-linked antibody (1:2000; Cell Signaling) for 1 h at room temperature.

    Article Title: Fluorescence Correlation Spectroscopy Reveals Efficient Cytosolic Delivery of Protein Cargo by Cell-Permeant Miniature Proteins
    Article Snippet: .. SNAP-tag was detected by incubating the membrane with an anti-SNAP-tag antibody (New England Biolabs, Ipswich, MA) followed by incubation with an HRP-linked anti-Rabbit IgG antibody (Cell Signaling Technology, Danvers, MA). .. The HRP signal was developed using Clarity Western ECL Substrates (Bio-Rad, Hercules, CA).

    Article Title: Hepatitis B virus PreS2-mutant large surface antigen activates store-operated calcium entry and promotes chromosome instability
    Article Snippet: The slides were then incubated with mouse anti-preS1 for 1 hr and assessed using the EnVision+ System-HRP kit (DAKO, Carpintena, CA, USA). .. The following primary antibodies were used in this study: mouse anti-preS1 (a gift from Ningshao Xia, Xiamen University, China), rabbit anti-SNAP tag (P9310S, New England Biolabs, MA, USA), rabbit anti-STIM1 (PA5-23623, Thermo Scientific, IL, USA), and mouse anti-γ-tubulin (sc-17787, Santa Cruz, Dallas, Texas, USA).

    Article Title: The novel function of tumor protein D54 in regulating pyruvate dehydrogenase and metformin cytotoxicity in breast cancer
    Article Snippet: Following this incubation, membranes were washed in 1× TBST for 30 min, followed by incubation with secondary antibody for an additional hour. .. The antibodies used were as follows: TPD54 (Abcam, AB194938), Pyruvate dehydrogenase (PDH) WB antibody cocktail (Abcam, ab110416), NDUFB8 antibody [20E9DH10C12] (Abcam, AB110242), PDH E1α (Abcam, ab110334), PDH E1α (Proteintech, 18068-1-AP), PDH E1α (Proteintech, 66,119-1-Ig), PhosphoS232 PDH E1α (LSBio, LS-C265964), PhosphoS293 PDH E1α (Novus, NB110-93479), phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb (Cell signaling, Cat#9718), vinculin (Cell signaling, Cat#4650), Actin (Cell signaling, cat# 3700), Cleaved PARP (Cell signaling, Cat#5625), ubiquitin (Abcam, ab7780), PDK1 (Santa Cruz,sc36203), PDK2 (Santa Cruz, sc517284), SNAP-Tag (New England Biolab, p9310s), mCherry (Sigma, SAB2702291), Peroxidase-AffiniPure F(ab′)2 Fragment Goat Anti-Rabbit IgG, Fc Fragment Specific (Jackson ImmunoResearch, 111-036-046), Peroxidase-AffiniPure F(ab′)2 Fragment Goat Anti-Mouse IgG, Fc Fragment Specific (Jackson ImmunoResearch, 115-036-071), Peroxidase-IgG Fraction Monoclonal Mouse Anti-Rabbit IgG, Light Chain Specific (Jackson ImmunoResearch, 211-032-171), and Peroxidase-AffiniPure Goat Anti-Mouse IgG, Light Chain Specific (Jackson ImmunoResearch, 115-035-174).

    Expressing:

    Article Title: Regulating G protein-coupled receptors by topological inversion
    Article Snippet: Materials We obtained anti-human CCR5 45531 from R and D Systems (Minneapolis, MN), anti-actin from Sigma Aldrich (St. Louis, MO), anti-Giantin 924302 from Biolegend (San Diego, CA), Alexa Fluor 488 FluoroNanogold goat anti-mouse IgG Fab from Nanoprobes.com (Yaphank, NY), AffiniPure Donkey Anti-Rabbit IgG (H + L) from Jackson ImmunoResearch (West Grove, PA), Alexa Fluor 488 goat Anti-Mouse IgG (H + L) from Invitrogen (Carlsbad, CA), Anti-SNAP-tag Antibody (Polyclonal) from New England Biolabs (Ipswichm, MA). .. Hybridoma cells expressing anti-Myc 9E10 were obtained from ATCC.

    Western Blot:

    Article Title: Agonist-induced membrane nanodomain clustering drives GLP-1 receptor responses in pancreatic beta cells
    Article Snippet: .. Antibodies for western blotting were anti-flotillin-1 mouse monoclonal antibody, catalogue no. sc-74566, Santa Cruz Biotechnology (1:200 dilution); anti-flotillin-1 rat monoclonal antibody, catalogue no. 849802, BioLegend (1:100 dilution); anti-SNAP-tag rabbit polyclonal antibody, catalogue no. P9310S, New England Biolabs (1:500 dilution); anti-GFP mouse monoclonal antibody, catalogue no. G6795, Sigma (1:1,000 dilution); anti-human transferrin receptor mouse monoclonal antibody, catalogue no. G1/221/12, Developmental Studies Hybridoma Bank (DSHB; 0.2 μg/ml); anti-human GLP-1R mouse monoclonal antibody, catalogue no. Mab 3F52, DSHB (0.2 μg/ml); anti-mouse GLP-1R mouse monoclonal antibody, catalogue no. Mab 7F38, DSHB (0.2 μg/ml); and anti-α-tubulin mouse monoclonal antibody, catalogue no. T5168, Sigma (1:1000 dilution). ..

    Article Title: Fluorescence Correlation Spectroscopy Reveals Efficient Cytosolic Delivery of Protein Cargo by Cell-Permeant Miniature Proteins
    Article Snippet: The gel was analyzed by in-gel fluorescence scanning (Typhoon FLA 7000) prior to transfer onto PVDF membranes for Western blot analysis. .. SNAP-tag was detected by incubating the membrane with an anti-SNAP-tag antibody (New England Biolabs, Ipswich, MA) followed by incubation with an HRP-linked anti-Rabbit IgG antibody (Cell Signaling Technology, Danvers, MA).

    Article Title: Molecular basis of signaling specificity between GIRK channels and GPCRs
    Article Snippet: .. Western Blot was performed using an anti-SNAP-tag (NEB), anti-HaloTag (Promega), anti-Gαi1 (abcam, ab140125), anti-Gαi2 (abcam, ab157204), anti-Gαo (Santa Cruz Biotechnology, sc-13532), or anti-Gαs (Santa Cruz Biotechnology, sc-383). .. Untransfected HEK-293T cells or HEK-293T cells transfected with βARs were cultured in 12-well plates for 20–24 hr.

    Article Title: The novel function of tumor protein D54 in regulating pyruvate dehydrogenase and metformin cytotoxicity in breast cancer
    Article Snippet: .. The antibodies used were as follows: TPD54 (Abcam, AB194938), Pyruvate dehydrogenase (PDH) WB antibody cocktail (Abcam, ab110416), NDUFB8 antibody [20E9DH10C12] (Abcam, AB110242), PDH E1α (Abcam, ab110334), PDH E1α (Proteintech, 18068-1-AP), PDH E1α (Proteintech, 66,119-1-Ig), PhosphoS232 PDH E1α (LSBio, LS-C265964), PhosphoS293 PDH E1α (Novus, NB110-93479), phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb (Cell signaling, Cat#9718), vinculin (Cell signaling, Cat#4650), Actin (Cell signaling, cat# 3700), Cleaved PARP (Cell signaling, Cat#5625), ubiquitin (Abcam, ab7780), PDK1 (Santa Cruz,sc36203), PDK2 (Santa Cruz, sc517284), SNAP-Tag (New England Biolab, p9310s), mCherry (Sigma, SAB2702291), Peroxidase-AffiniPure F(ab′)2 Fragment Goat Anti-Rabbit IgG, Fc Fragment Specific (Jackson ImmunoResearch, 111-036-046), Peroxidase-AffiniPure F(ab′)2 Fragment Goat Anti-Mouse IgG, Fc Fragment Specific (Jackson ImmunoResearch, 115-036-071), Peroxidase-IgG Fraction Monoclonal Mouse Anti-Rabbit IgG, Light Chain Specific (Jackson ImmunoResearch, 211-032-171), and Peroxidase-AffiniPure Goat Anti-Mouse IgG, Light Chain Specific (Jackson ImmunoResearch, 115-035-174). .. Additional antibodies included Goat Anti-Mouse IgG (H + L) (Alexa Fluor 594) (Abcam, ab150116), Goat Anti-Rabbit IgG (H + L) (Alexa Fluor 488) (Abcam, ab150077), Anti-rabbit IgG, HRP-linked antibody (Sigma, 7074S), and Anti-mouse IgG HRP-linked antibody (Sigma, 7076S).

    Modification:

    Article Title: RIP2 filament formation is required for NOD2 dependent NF-κB signalling
    Article Snippet: Immuno-gold labelling For immuno-gold labelling experiments, we modified protocol illustrated in Lu, A et al., 2015 , as follows. .. After removal of excess, grid was transferred to 30 µl drops of same solution containing either the rabbit antibody anti-SNAP at dilution 1:2000 (P9310, New England Biolabs), or rabbit anti-NOD2 at dilution 1:1000 (ab197030, Abcam) or no antibody (control sample C2).

    Double Immunofluorescence Staining:

    Article Title: Hepatitis B virus PreS2-mutant large surface antigen activates store-operated calcium entry and promotes chromosome instability
    Article Snippet: Paragraph title: Immunohistochemical and double immunofluorescence staining ... The following primary antibodies were used in this study: mouse anti-preS1 (a gift from Ningshao Xia, Xiamen University, China), rabbit anti-SNAP tag (P9310S, New England Biolabs, MA, USA), rabbit anti-STIM1 (PA5-23623, Thermo Scientific, IL, USA), and mouse anti-γ-tubulin (sc-17787, Santa Cruz, Dallas, Texas, USA).

    Electron Microscopy:

    Article Title: RIP2 filament formation is required for NOD2 dependent NF-κB signalling
    Article Snippet: For immuno-gold labelling, 6 µl of sample were applied to glow-discharged carbon coated copper grid (300 mesh, Electron Microscopy Science) and let adsorb for 30 s. Excess sample was blot off on filter paper, and grid was let shaking 10 min in 30 µl drop of PBS + 0.1% (w/v) BSA. .. After removal of excess, grid was transferred to 30 µl drops of same solution containing either the rabbit antibody anti-SNAP at dilution 1:2000 (P9310, New England Biolabs), or rabbit anti-NOD2 at dilution 1:1000 (ab197030, Abcam) or no antibody (control sample C2).

    Transfection:

    Article Title: Molecular basis of signaling specificity between GIRK channels and GPCRs
    Article Snippet: Untransfected HEK-293T cells or HEK-293T cells transiently transfected with GPCR-GIRK4 concatemers were centrifuged and mixed with an equal volume of loading buffer containing 4% SDS and 10% β-mercaptoethanol. .. Western Blot was performed using an anti-SNAP-tag (NEB), anti-HaloTag (Promega), anti-Gαi1 (abcam, ab140125), anti-Gαi2 (abcam, ab157204), anti-Gαo (Santa Cruz Biotechnology, sc-13532), or anti-Gαs (Santa Cruz Biotechnology, sc-383).

    Protease Inhibitor:

    Article Title: The anti-tumor drug 2-hydroxyoleic acid (Minerval) stimulates signaling and retrograde transport
    Article Snippet: Immunoblotting Treated cells were washed with PBS and lysed (0.1 M NaCl, 10 mM Na2 HPO4 , pH 7.4, 1 mM EDTA, 1% Triton X-100, 60 mM n-octyl β-D-glucopyranoside, and cOmplete EDTA-free Protease Inhibitor Cocktail (Roche)). .. The following antibodies were used: phospho-AKT1Ser473 (#9271), AKT1 (#4691), phospho-RPS6KBThr389 (#9205), RPS6KB (#9202), phospho-Erk/ MAPK1/3Thr202/Tyr204 (#9106), Erk/MAPK1/3 (#9102), phospho-p38/MAPK14Thr180/Tyr182 (#9211), phospho-JUN (#9164), ERBB2Tyr877 (#2241), PERK /EIF2AK3 (#5683) all from Cell Signaling Technology, and p-38/MAPK14 (#612168, Transduction Laboratories), GFP (clone 3H9, ChromoTek), phospho-EGFRTyr1173 (#ab24912, Abcam), GAPDH (#ab9482, Abcam), ACTIN (#CLT9001, Cedarlane), SNAP (#P9310S, New England Biolabs), Shiga toxin (#STX1-3C10, Toxin Technology), ricin (#C86370M, Meridian), and XBP1s (#647502, BioLegend).

    Article Title: Fluorescence Correlation Spectroscopy Reveals Efficient Cytosolic Delivery of Protein Cargo by Cell-Permeant Miniature Proteins
    Article Snippet: This step was repeated to perform a second wash. After the second DPBS wash, the cells were resuspended in 1 mL of precooled buffered isotonic sucrose buffer (290 mM sucrose, 10 mM imidazole pH 7.0, 1 mM DTT, and 1 cOmplete protease inhibitor cocktail per 10 mL buffer) and repelleted at 500g for 2 min. Next, the cells were suspended in 150 μL of isotonic sucrose, transferred to 0.5 mL microtubes containing 1.4 mm ceramic beads (Omni International) and homogenized using a Bead Ruptor 4 (Omni International) at speed 1 for 8 s. The homogenized cells were transferred to polycarbonate ultracentrifuge tubes and centrifuged at 350 kg for 30 min at 4 °C to isolate the cytosolic fraction. .. SNAP-tag was detected by incubating the membrane with an anti-SNAP-tag antibody (New England Biolabs, Ipswich, MA) followed by incubation with an HRP-linked anti-Rabbit IgG antibody (Cell Signaling Technology, Danvers, MA).

    other:

    Article Title: Unique Roles of β-Arrestin in GPCR Trafficking Revealed by Photoinducible Dimerizers
    Article Snippet: SNAP-Surface Alexa Fluor 647 and anti-SNAP-tag antibodies were obtained from New England Biolabs (USA).

    Article Title: Unique Roles of β-Arrestin in GPCR Trafficking Revealed by Photoinducible Dimerizers
    Article Snippet: Materials and cDNA Construction SNAP-Surface Alexa Fluor 647 and anti-SNAP-tag antibodies were obtained from New England Biolabs (USA).

    Imaging:

    Article Title: The anti-tumor drug 2-hydroxyoleic acid (Minerval) stimulates signaling and retrograde transport
    Article Snippet: The membrane was blocked in 7.5% milk for 45 minutes, followed by overnight incubation with the indicated antibodies in 5% BSA, 45 minutes incubation with HRP-conjugated secondary antibodies and detection with SuperSignal West Dura Extended Duration Substrate (Thermo Scientific) in a ChemiDoc Imaging System (Bio-Rad). .. The following antibodies were used: phospho-AKT1Ser473 (#9271), AKT1 (#4691), phospho-RPS6KBThr389 (#9205), RPS6KB (#9202), phospho-Erk/ MAPK1/3Thr202/Tyr204 (#9106), Erk/MAPK1/3 (#9102), phospho-p38/MAPK14Thr180/Tyr182 (#9211), phospho-JUN (#9164), ERBB2Tyr877 (#2241), PERK /EIF2AK3 (#5683) all from Cell Signaling Technology, and p-38/MAPK14 (#612168, Transduction Laboratories), GFP (clone 3H9, ChromoTek), phospho-EGFRTyr1173 (#ab24912, Abcam), GAPDH (#ab9482, Abcam), ACTIN (#CLT9001, Cedarlane), SNAP (#P9310S, New England Biolabs), Shiga toxin (#STX1-3C10, Toxin Technology), ricin (#C86370M, Meridian), and XBP1s (#647502, BioLegend).

    Article Title: The novel function of tumor protein D54 in regulating pyruvate dehydrogenase and metformin cytotoxicity in breast cancer
    Article Snippet: Proteins were detected by SuperSignal™ West Dura Extended Duration Substrate (Cat#34075) using the ChemiDoc™ Touch Imaging System. .. The antibodies used were as follows: TPD54 (Abcam, AB194938), Pyruvate dehydrogenase (PDH) WB antibody cocktail (Abcam, ab110416), NDUFB8 antibody [20E9DH10C12] (Abcam, AB110242), PDH E1α (Abcam, ab110334), PDH E1α (Proteintech, 18068-1-AP), PDH E1α (Proteintech, 66,119-1-Ig), PhosphoS232 PDH E1α (LSBio, LS-C265964), PhosphoS293 PDH E1α (Novus, NB110-93479), phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb (Cell signaling, Cat#9718), vinculin (Cell signaling, Cat#4650), Actin (Cell signaling, cat# 3700), Cleaved PARP (Cell signaling, Cat#5625), ubiquitin (Abcam, ab7780), PDK1 (Santa Cruz,sc36203), PDK2 (Santa Cruz, sc517284), SNAP-Tag (New England Biolab, p9310s), mCherry (Sigma, SAB2702291), Peroxidase-AffiniPure F(ab′)2 Fragment Goat Anti-Rabbit IgG, Fc Fragment Specific (Jackson ImmunoResearch, 111-036-046), Peroxidase-AffiniPure F(ab′)2 Fragment Goat Anti-Mouse IgG, Fc Fragment Specific (Jackson ImmunoResearch, 115-036-071), Peroxidase-IgG Fraction Monoclonal Mouse Anti-Rabbit IgG, Light Chain Specific (Jackson ImmunoResearch, 211-032-171), and Peroxidase-AffiniPure Goat Anti-Mouse IgG, Light Chain Specific (Jackson ImmunoResearch, 115-035-174).

    Inverted Microscopy:

    Article Title: Hepatitis B virus PreS2-mutant large surface antigen activates store-operated calcium entry and promotes chromosome instability
    Article Snippet: The images were acquired with a Zeiss LSM780 confocal microscope or an automated Leica DMI6000 inverted microscope equipped with an HCX PL FL 20x/NA0.4 objective and an EMCCD camera (Andor Luca R, Belfast, UK) as indicated. .. The following primary antibodies were used in this study: mouse anti-preS1 (a gift from Ningshao Xia, Xiamen University, China), rabbit anti-SNAP tag (P9310S, New England Biolabs, MA, USA), rabbit anti-STIM1 (PA5-23623, Thermo Scientific, IL, USA), and mouse anti-γ-tubulin (sc-17787, Santa Cruz, Dallas, Texas, USA).

    Recombinant:

    Article Title: Regulating G protein-coupled receptors by topological inversion
    Article Snippet: Materials We obtained anti-human CCR5 45531 from R and D Systems (Minneapolis, MN), anti-actin from Sigma Aldrich (St. Louis, MO), anti-Giantin 924302 from Biolegend (San Diego, CA), Alexa Fluor 488 FluoroNanogold goat anti-mouse IgG Fab from Nanoprobes.com (Yaphank, NY), AffiniPure Donkey Anti-Rabbit IgG (H + L) from Jackson ImmunoResearch (West Grove, PA), Alexa Fluor 488 goat Anti-Mouse IgG (H + L) from Invitrogen (Carlsbad, CA), Anti-SNAP-tag Antibody (Polyclonal) from New England Biolabs (Ipswichm, MA). .. Recombinant human CCL5 was obtained from R and D Systems (Minneapolis, MN).

    Fluorescence:

    Article Title: Fluorescence Correlation Spectroscopy Reveals Efficient Cytosolic Delivery of Protein Cargo by Cell-Permeant Miniature Proteins
    Article Snippet: The gel was analyzed by in-gel fluorescence scanning (Typhoon FLA 7000) prior to transfer onto PVDF membranes for Western blot analysis. .. SNAP-tag was detected by incubating the membrane with an anti-SNAP-tag antibody (New England Biolabs, Ipswich, MA) followed by incubation with an HRP-linked anti-Rabbit IgG antibody (Cell Signaling Technology, Danvers, MA).

    Mutagenesis:

    Article Title: Corticotropin releasing hormone receptor 2 exacerbates chronic cardiac dysfunction
    Article Snippet: Luciferase assay SNAP antibody (P9310S) and the pSNAPf plasmid were obtained from New England Biolabs. .. Luciferase reporters for cAMP response element (CRE), nuclear factor of activated T-cells response element (NFAT-RE), serum response element (SRE), and serum response factor response element (SRF-RE, a mutant form of SRE) were used to assess Gs, Gq, Gi, and G12 activation, respectively ( ).

    Isolation:

    Article Title: Heparan sulfate antagonism alters bone morphogenetic protein signaling and receptor dynamics, suggesting a mechanism in hereditary multiple exostoses
    Article Snippet: Paragraph title: DRM isolation ... Membranes were blocked in TBST and incubated overnight at 4 °C with anti-SNAP-tag (1:1000; New England Biolabs) and caveolin-1 (1:1000; Cell Signaling).

    Immunohistochemistry:

    Article Title: Hepatitis B virus PreS2-mutant large surface antigen activates store-operated calcium entry and promotes chromosome instability
    Article Snippet: Paragraph title: Immunohistochemical and double immunofluorescence staining ... The following primary antibodies were used in this study: mouse anti-preS1 (a gift from Ningshao Xia, Xiamen University, China), rabbit anti-SNAP tag (P9310S, New England Biolabs, MA, USA), rabbit anti-STIM1 (PA5-23623, Thermo Scientific, IL, USA), and mouse anti-γ-tubulin (sc-17787, Santa Cruz, Dallas, Texas, USA).

    Microscopy:

    Article Title: Hepatitis B virus PreS2-mutant large surface antigen activates store-operated calcium entry and promotes chromosome instability
    Article Snippet: The images were acquired with a Zeiss LSM780 confocal microscope or an automated Leica DMI6000 inverted microscope equipped with an HCX PL FL 20x/NA0.4 objective and an EMCCD camera (Andor Luca R, Belfast, UK) as indicated. .. The following primary antibodies were used in this study: mouse anti-preS1 (a gift from Ningshao Xia, Xiamen University, China), rabbit anti-SNAP tag (P9310S, New England Biolabs, MA, USA), rabbit anti-STIM1 (PA5-23623, Thermo Scientific, IL, USA), and mouse anti-γ-tubulin (sc-17787, Santa Cruz, Dallas, Texas, USA).

    Purification:

    Article Title: RIP2 filament formation is required for NOD2 dependent NF-κB signalling
    Article Snippet: Immuno-gold labelling was applied after MBP and HIS-SUMO tags cleavage without further purification. .. After removal of excess, grid was transferred to 30 µl drops of same solution containing either the rabbit antibody anti-SNAP at dilution 1:2000 (P9310, New England Biolabs), or rabbit anti-NOD2 at dilution 1:1000 (ab197030, Abcam) or no antibody (control sample C2).

    Labeling:

    Article Title: Clathrin promotes centrosome integrity in early mitosis through stabilization of centrosomal ch-TOG
    Article Snippet: Labeled proteins were detected using the ECL-Plus reagent (GE Healthcare). .. For detection of the SNAP-tag and CHC17 by blotting, the anti–SNAP-tag antibody (New England Biolabs, Inc.) and TD.1 antibody were used, respectively.

    Chloramphenicol Acetyltransferase Assay:

    Article Title: The novel function of tumor protein D54 in regulating pyruvate dehydrogenase and metformin cytotoxicity in breast cancer
    Article Snippet: .. The antibodies used were as follows: TPD54 (Abcam, AB194938), Pyruvate dehydrogenase (PDH) WB antibody cocktail (Abcam, ab110416), NDUFB8 antibody [20E9DH10C12] (Abcam, AB110242), PDH E1α (Abcam, ab110334), PDH E1α (Proteintech, 18068-1-AP), PDH E1α (Proteintech, 66,119-1-Ig), PhosphoS232 PDH E1α (LSBio, LS-C265964), PhosphoS293 PDH E1α (Novus, NB110-93479), phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb (Cell signaling, Cat9718), vinculin (Cell signaling, Cat#4650), Actin (Cell signaling, cat# 3700), Cleaved PARP (Cell signaling, Cat#5625), ubiquitin (Abcam, ab7780), PDK1 (Santa Cruz,sc36203), PDK2 (Santa Cruz, sc517284), SNAP-Tag (New England Biolab, p9310s), mCherry (Sigma, SAB2702291), Peroxidase-AffiniPure F(ab′)2 Fragment Goat Anti-Rabbit IgG, Fc Fragment Specific (Jackson ImmunoResearch, 111-036-046), Peroxidase-AffiniPure F(ab′)2 Fragment Goat Anti-Mouse IgG, Fc Fragment Specific (Jackson ImmunoResearch, 115-036-071), Peroxidase-IgG Fraction Monoclonal Mouse Anti-Rabbit IgG, Light Chain Specific (Jackson ImmunoResearch, 211-032-171), and Peroxidase-AffiniPure Goat Anti-Mouse IgG, Light Chain Specific (Jackson ImmunoResearch, 115-035-174). .. Additional antibodies included Goat Anti-Mouse IgG (H + L) (Alexa Fluor 594) (Abcam, ab150116), Goat Anti-Rabbit IgG (H + L) (Alexa Fluor 488) (Abcam, ab150077), Anti-rabbit IgG, HRP-linked antibody (Sigma, 7074S), and Anti-mouse IgG HRP-linked antibody (Sigma, 7076S).

    SDS Page:

    Article Title: Clathrin promotes centrosome integrity in early mitosis through stabilization of centrosomal ch-TOG
    Article Snippet: Immunoblot analysis Cell lysates were resolved by SDS-PAGE (NuPage Bis-Tris 4–12% SDS-acrylamide gels; Invitrogen), transferred to nitrocellulose membranes (EMD Millipore), and exposed to primary (1–5 µg/ml) and peroxidase-conjugated secondary antibodies. .. For detection of the SNAP-tag and CHC17 by blotting, the anti–SNAP-tag antibody (New England Biolabs, Inc.) and TD.1 antibody were used, respectively.

    Article Title: The anti-tumor drug 2-hydroxyoleic acid (Minerval) stimulates signaling and retrograde transport
    Article Snippet: The cleared lysates were separated by 4–20% SDS-PAGE and transferred to a PVDF membrane. .. The following antibodies were used: phospho-AKT1Ser473 (#9271), AKT1 (#4691), phospho-RPS6KBThr389 (#9205), RPS6KB (#9202), phospho-Erk/ MAPK1/3Thr202/Tyr204 (#9106), Erk/MAPK1/3 (#9102), phospho-p38/MAPK14Thr180/Tyr182 (#9211), phospho-JUN (#9164), ERBB2Tyr877 (#2241), PERK /EIF2AK3 (#5683) all from Cell Signaling Technology, and p-38/MAPK14 (#612168, Transduction Laboratories), GFP (clone 3H9, ChromoTek), phospho-EGFRTyr1173 (#ab24912, Abcam), GAPDH (#ab9482, Abcam), ACTIN (#CLT9001, Cedarlane), SNAP (#P9310S, New England Biolabs), Shiga toxin (#STX1-3C10, Toxin Technology), ricin (#C86370M, Meridian), and XBP1s (#647502, BioLegend).

    Article Title: Agonist-induced membrane nanodomain clustering drives GLP-1 receptor responses in pancreatic beta cells
    Article Snippet: Paragraph title: SDS-PAGE and western blotting ... Antibodies for western blotting were anti-flotillin-1 mouse monoclonal antibody, catalogue no. sc-74566, Santa Cruz Biotechnology (1:200 dilution); anti-flotillin-1 rat monoclonal antibody, catalogue no. 849802, BioLegend (1:100 dilution); anti-SNAP-tag rabbit polyclonal antibody, catalogue no. P9310S, New England Biolabs (1:500 dilution); anti-GFP mouse monoclonal antibody, catalogue no. G6795, Sigma (1:1,000 dilution); anti-human transferrin receptor mouse monoclonal antibody, catalogue no. G1/221/12, Developmental Studies Hybridoma Bank (DSHB; 0.2 μg/ml); anti-human GLP-1R mouse monoclonal antibody, catalogue no. Mab 3F52, DSHB (0.2 μg/ml); anti-mouse GLP-1R mouse monoclonal antibody, catalogue no. Mab 7F38, DSHB (0.2 μg/ml); and anti-α-tubulin mouse monoclonal antibody, catalogue no. T5168, Sigma (1:1000 dilution).

    Article Title: Fluorescence Correlation Spectroscopy Reveals Efficient Cytosolic Delivery of Protein Cargo by Cell-Permeant Miniature Proteins
    Article Snippet: Next, the cytosolic fractions were boiled in gel loading buffer and separated by SDS-PAGE. .. SNAP-tag was detected by incubating the membrane with an anti-SNAP-tag antibody (New England Biolabs, Ipswich, MA) followed by incubation with an HRP-linked anti-Rabbit IgG antibody (Cell Signaling Technology, Danvers, MA).

    Article Title: Molecular basis of signaling specificity between GIRK channels and GPCRs
    Article Snippet: Samples were then run using standard SDS-PAGE procedures on Invitrogen NuPAGE 4–12% Bis-Tris gels and transferred onto PVDF membranes. .. Western Blot was performed using an anti-SNAP-tag (NEB), anti-HaloTag (Promega), anti-Gαi1 (abcam, ab140125), anti-Gαi2 (abcam, ab157204), anti-Gαo (Santa Cruz Biotechnology, sc-13532), or anti-Gαs (Santa Cruz Biotechnology, sc-383).

    Plasmid Preparation:

    Article Title: Corticotropin releasing hormone receptor 2 exacerbates chronic cardiac dysfunction
    Article Snippet: .. Luciferase assay SNAP antibody (P9310S) and the pSNAPf plasmid were obtained from New England Biolabs. .. The vectors pGL4.29[luc2P/CRE/Hygro], pGL4.30[luc2P/NFAT-RE/Hygro], pGL4.33[luc2P/SRE/Hygro], and pGL4.34[luc2P/SRF-RE/Hygro] were purchased from Promega.

    Software:

    Article Title: Clathrin promotes centrosome integrity in early mitosis through stabilization of centrosomal ch-TOG
    Article Snippet: Molecular mass markers (Rainbow; GE Healthcare) were used for calibration, and protein band intensities were quantified using Quantity One (Bio-Rad Laboratories) or ImageJ (version 1.45c) software. .. For detection of the SNAP-tag and CHC17 by blotting, the anti–SNAP-tag antibody (New England Biolabs, Inc.) and TD.1 antibody were used, respectively.

    Article Title: The anti-tumor drug 2-hydroxyoleic acid (Minerval) stimulates signaling and retrograde transport
    Article Snippet: The signal intensities were quantified by the Quantity One software (Bio-Rad) and were normalized to the loading control. .. The following antibodies were used: phospho-AKT1Ser473 (#9271), AKT1 (#4691), phospho-RPS6KBThr389 (#9205), RPS6KB (#9202), phospho-Erk/ MAPK1/3Thr202/Tyr204 (#9106), Erk/MAPK1/3 (#9102), phospho-p38/MAPK14Thr180/Tyr182 (#9211), phospho-JUN (#9164), ERBB2Tyr877 (#2241), PERK /EIF2AK3 (#5683) all from Cell Signaling Technology, and p-38/MAPK14 (#612168, Transduction Laboratories), GFP (clone 3H9, ChromoTek), phospho-EGFRTyr1173 (#ab24912, Abcam), GAPDH (#ab9482, Abcam), ACTIN (#CLT9001, Cedarlane), SNAP (#P9310S, New England Biolabs), Shiga toxin (#STX1-3C10, Toxin Technology), ricin (#C86370M, Meridian), and XBP1s (#647502, BioLegend).

    Homogenization:

    Article Title: Heparan sulfate antagonism alters bone morphogenetic protein signaling and receptor dynamics, suggesting a mechanism in hereditary multiple exostoses
    Article Snippet: Following homogenization, 250 μl of 2% Triton X-100 was added to 250 μl of the cell homogenate and placed on ice for 30 min. For the step sucrose gradient, 1.25 ml of 56% sucrose was added to the sample for a final percentage of 40% sucrose. .. Membranes were blocked in TBST and incubated overnight at 4 °C with anti-SNAP-tag (1:1000; New England Biolabs) and caveolin-1 (1:1000; Cell Signaling).

    Activation Assay:

    Article Title: Corticotropin releasing hormone receptor 2 exacerbates chronic cardiac dysfunction
    Article Snippet: Luciferase assay SNAP antibody (P9310S) and the pSNAPf plasmid were obtained from New England Biolabs. .. Luciferase reporters for cAMP response element (CRE), nuclear factor of activated T-cells response element (NFAT-RE), serum response element (SRE), and serum response factor response element (SRF-RE, a mutant form of SRE) were used to assess Gs, Gq, Gi, and G12 activation, respectively ( ).

    Fractionation:

    Article Title: Fluorescence Correlation Spectroscopy Reveals Efficient Cytosolic Delivery of Protein Cargo by Cell-Permeant Miniature Proteins
    Article Snippet: Paragraph title: Cytosolic Fractionation Assay ... SNAP-tag was detected by incubating the membrane with an anti-SNAP-tag antibody (New England Biolabs, Ipswich, MA) followed by incubation with an HRP-linked anti-Rabbit IgG antibody (Cell Signaling Technology, Danvers, MA).

    Staining:

    Article Title: Hepatitis B virus PreS2-mutant large surface antigen activates store-operated calcium entry and promotes chromosome instability
    Article Snippet: For double immunofluorescence staining, the cells were fixed and stained with the indicated primary antibodies for 1 hr, followed by secondary antibodies conjugated with Alexa-488, Alexa-594, or Alexa-647 (Molecular Probes). .. The following primary antibodies were used in this study: mouse anti-preS1 (a gift from Ningshao Xia, Xiamen University, China), rabbit anti-SNAP tag (P9310S, New England Biolabs, MA, USA), rabbit anti-STIM1 (PA5-23623, Thermo Scientific, IL, USA), and mouse anti-γ-tubulin (sc-17787, Santa Cruz, Dallas, Texas, USA).

    Article Title: RIP2 filament formation is required for NOD2 dependent NF-κB signalling
    Article Snippet: After removal of excess, grid was transferred to 30 µl drops of same solution containing either the rabbit antibody anti-SNAP at dilution 1:2000 (P9310, New England Biolabs), or rabbit anti-NOD2 at dilution 1:1000 (ab197030, Abcam) or no antibody (control sample C2). .. After washing a second time, the grid was stained with 2% (w/v) uranyl acetate and images were recorded on a JEOL 1200 at 16,000x or 25,000x magnification.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    New England Biolabs anti snap tag
    Surfen promotes recruitment of BMPRII population to lipid raft domains. Vehicle-treated ( A and B ) and surfen-treated Ad-293 cells ( C and D ) expressing <t>SNAP-BMPRII</t> or SNAP-BMPRIa were homogenized and fractionated by sucrose gradient ultracentrifugation. Fractions were blotted with anti-SNAP or <t>anti-caveolin</t> 1 antibodies. A , in vehicle-treated cells, the BMPRII population is distributed in both lipid raft and nonlipid raft domains. C , in surfen-treated cells, there is a robust shift in BMPRII population to lipid raft domains, depicted in particular in fraction 1. B , in vehicle-treated cells, the BMPRIa population is mostly in the lipid raft domains. D , in surfen-treated cells, the majority of the BMPRIa population is seen in lipid raft domains, but there are a significant amount of receptors in the nonlipid raft domains compared with the control cells. E and F , quantification of receptor population in either DRM or non-DRM (*, p
    Anti Snap Tag, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti snap tag/product/New England Biolabs
    Average 99 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    anti snap tag - by Bioz Stars, 2020-03
    99/100 stars
      Buy from Supplier

    Image Search Results


    Surfen promotes recruitment of BMPRII population to lipid raft domains. Vehicle-treated ( A and B ) and surfen-treated Ad-293 cells ( C and D ) expressing SNAP-BMPRII or SNAP-BMPRIa were homogenized and fractionated by sucrose gradient ultracentrifugation. Fractions were blotted with anti-SNAP or anti-caveolin 1 antibodies. A , in vehicle-treated cells, the BMPRII population is distributed in both lipid raft and nonlipid raft domains. C , in surfen-treated cells, there is a robust shift in BMPRII population to lipid raft domains, depicted in particular in fraction 1. B , in vehicle-treated cells, the BMPRIa population is mostly in the lipid raft domains. D , in surfen-treated cells, the majority of the BMPRIa population is seen in lipid raft domains, but there are a significant amount of receptors in the nonlipid raft domains compared with the control cells. E and F , quantification of receptor population in either DRM or non-DRM (*, p

    Journal: The Journal of Biological Chemistry

    Article Title: Heparan sulfate antagonism alters bone morphogenetic protein signaling and receptor dynamics, suggesting a mechanism in hereditary multiple exostoses

    doi: 10.1074/jbc.RA117.000264

    Figure Lengend Snippet: Surfen promotes recruitment of BMPRII population to lipid raft domains. Vehicle-treated ( A and B ) and surfen-treated Ad-293 cells ( C and D ) expressing SNAP-BMPRII or SNAP-BMPRIa were homogenized and fractionated by sucrose gradient ultracentrifugation. Fractions were blotted with anti-SNAP or anti-caveolin 1 antibodies. A , in vehicle-treated cells, the BMPRII population is distributed in both lipid raft and nonlipid raft domains. C , in surfen-treated cells, there is a robust shift in BMPRII population to lipid raft domains, depicted in particular in fraction 1. B , in vehicle-treated cells, the BMPRIa population is mostly in the lipid raft domains. D , in surfen-treated cells, the majority of the BMPRIa population is seen in lipid raft domains, but there are a significant amount of receptors in the nonlipid raft domains compared with the control cells. E and F , quantification of receptor population in either DRM or non-DRM (*, p

    Article Snippet: Membranes were blocked in TBST and incubated overnight at 4 °C with anti-SNAP-tag (1:1000; New England Biolabs) and caveolin-1 (1:1000; Cell Signaling).

    Techniques: Expressing