Journal: bioRxiv

Article Title: Remodelled Ribosomes Synthesise a Specific Proteome in Proliferating Plant Tissue during Cold

doi: 10.1101/2022.11.28.518201

Figure Lengend Snippet: Barley ribosomes from plant 15 N labelled proliferative tissue were purified and used to profile the rProteome. Abundances and isotopolog envelopes were recovered from the Mass Spectrometry data and used to calculate average ribosome relative stoichiometry of rProteins and their fractional synthesis rates. (a) rProtein substoichiometry; orange and reds are rProteins accumulated at optimal temperature and blues rProteins accumulated during cold in the ribosomal population. (b) Preferential synthesis of assembled rProteins during cold acclimation. (c) Preferential synthesis of assembled rProteins during control temperature. Preferential synthesis refers to peptides with significant changes ( P adj values < 0.05, n = 3, tested using a customized generalized linear model) in their fractional synthesis rates. . Volcano plot outlining the differences in mean peptide length produced with Lys-C or Trypsin, during an in silico protease digestion test, as proteases to digest the Arabidopsis signature plant ribosomal proteome. . Positive control and complete coverage of escherichia coli 70S ribosomal proteome from a commercially available preparation used to verify the ribosomal proteomics pipeline. . Ratio between 40S SSU and 60S LSU abundances across experimental samples

Article Snippet: The digestion buffer (25mM triethylammonium bicarbonate (TEAB)) contained the Lys-C protease (P8109S, NEB, Australia) at a 1:20 protease to protein ratio.

Techniques: Purification, Mass Spectrometry, Produced, In Silico, Positive Control

Journal: eLife

Article Title: The Nse5/6-like SIMC1-SLF2 complex localizes SMC5/6 to viral replication centers

doi: 10.7554/eLife.79676

Figure Lengend Snippet:

Article Snippet: peptide, recombinant protein , Endoproteinase LysC , New England BioLabs , P8109S , .

Techniques: Derivative Assay, CRISPR, Expressing, Transfection, Construct, Plasmid Preparation, Stable Transfection, Recombinant, Sequencing, Protease Inhibitor, Clone Assay, Software

Journal: eLife

Article Title: The Nse5/6-like SIMC1-SLF2 complex localizes SMC5/6 to viral replication centers

doi: 10.7554/eLife.79676

Figure Lengend Snippet:

Article Snippet: peptide, recombinant protein , Endoproteinase LysC , New England BioLabs , P8109S , .

Techniques: Derivative Assay, CRISPR, Expressing, Transfection, Construct, Plasmid Preparation, Stable Transfection, Recombinant, Sequencing, Protease Inhibitor, Clone Assay, Software

Journal: Cell reports

Article Title: The light chain of the L9 antibody is critical for binding circumsporozoite protein minor repeats and preventing malaria

doi: 10.1016/j.celrep.2022.110367

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: LysC , New England Biolabs , Cat# P8109S.

Techniques: Recombinant, Construct, SYBR Green Assay, Staining, Expressing, Crystallization Assay, Luciferase, Amplification, Software, In Vivo Imaging, Fluorescence, Microscopy