endoproteinase asp n asp n  (New England Biolabs)


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  • 92
    Name:
    Endoproteinase AspN
    Description:
    Endoproteinase AspN 50 ug
    Catalog Number:
    p8104s
    Price:
    80
    Size:
    50 ug
    Category:
    Proteases
    Buy from Supplier


    Structured Review

    New England Biolabs endoproteinase asp n asp n
    Endoproteinase AspN
    Endoproteinase AspN 50 ug
    https://www.bioz.com/result/endoproteinase asp n asp n/product/New England Biolabs
    Average 92 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    endoproteinase asp n asp n - by Bioz Stars, 2020-08
    92/100 stars

    Images

    1) Product Images from "Refinement of Glucagon-like Peptide 1 Docking to Its Intact Receptor Using Mid-region Photolabile Probes and Molecular Modeling"

    Article Title: Refinement of Glucagon-like Peptide 1 Docking to Its Intact Receptor Using Mid-region Photolabile Probes and Molecular Modeling

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M110.217901

    Endoproteinase Asp-N cleavage of the GLP1 receptor labeled with the Bpa 16 probe. Shown at the left is a diagram of the predicted sites of Asp-N cleavage of the GLP1 receptor along with the masses. Shown at the right is a representative autoradiograph
    Figure Legend Snippet: Endoproteinase Asp-N cleavage of the GLP1 receptor labeled with the Bpa 16 probe. Shown at the left is a diagram of the predicted sites of Asp-N cleavage of the GLP1 receptor along with the masses. Shown at the right is a representative autoradiograph

    Techniques Used: Labeling, Autoradiography

    Endoproteinase Asp-N cleavage of the GLP1 receptor labeled with the Bpa 20 probe. Shown at the left is a diagram of the predicted sites of Asp-N cleavage of the GLP1 receptor along with the masses. Shown at the right is a representative autoradiograph
    Figure Legend Snippet: Endoproteinase Asp-N cleavage of the GLP1 receptor labeled with the Bpa 20 probe. Shown at the left is a diagram of the predicted sites of Asp-N cleavage of the GLP1 receptor along with the masses. Shown at the right is a representative autoradiograph

    Techniques Used: Labeling, Autoradiography

    2) Product Images from "Molecular Basis of Glucagon-like Peptide 1 Docking to Its Intact Receptor Studied with Carboxyl-terminal Photolabile Probes *"

    Article Title: Molecular Basis of Glucagon-like Peptide 1 Docking to Its Intact Receptor Studied with Carboxyl-terminal Photolabile Probes *

    Journal:

    doi: 10.1074/jbc.M109.038109

    Further localization of the domain of Bpa 35 probe labeling. Left shows that endoproteinase Asp-N cleavage of the M r = 15,000 fragment resulting from Lys-C cleavage of the wild-type receptor yielded a band migrating at approximate M r = 4,000. Right shows
    Figure Legend Snippet: Further localization of the domain of Bpa 35 probe labeling. Left shows that endoproteinase Asp-N cleavage of the M r = 15,000 fragment resulting from Lys-C cleavage of the wild-type receptor yielded a band migrating at approximate M r = 4,000. Right shows

    Techniques Used: Labeling

    Related Articles

    Purification:

    Article Title: Improvement of the glycoproteomic toolbox with the discovery of a unique C-terminal cleavage specificity of flavastacin for N-glycosylated asparagine
    Article Snippet: .. Enzymes used for digestion were trypsin (Trypsin Sequencing Grade Modified; V5111) from Promega and endoproteinase AspN (AspN; P8104S) from New England Biolabs (Flavastacin, purified host cell protein, Uniprot Q47899; see the corresponding SDS-gel for purity of AspN (Flavastacin) in Supplementary Figure ). .. All buffer and solutions were prepared with deionized and purified water (dH2 O) using a Milli-Q water purification system (18.2 MΩ · cm−1 at 25°C, total organic carbon of 3 ppb) from Merck Millipore.

    SDS-Gel:

    Article Title: Improvement of the glycoproteomic toolbox with the discovery of a unique C-terminal cleavage specificity of flavastacin for N-glycosylated asparagine
    Article Snippet: .. Enzymes used for digestion were trypsin (Trypsin Sequencing Grade Modified; V5111) from Promega and endoproteinase AspN (AspN; P8104S) from New England Biolabs (Flavastacin, purified host cell protein, Uniprot Q47899; see the corresponding SDS-gel for purity of AspN (Flavastacin) in Supplementary Figure ). .. All buffer and solutions were prepared with deionized and purified water (dH2 O) using a Milli-Q water purification system (18.2 MΩ · cm−1 at 25°C, total organic carbon of 3 ppb) from Merck Millipore.

    Sequencing:

    Article Title: Hinge-Region O-Glycosylation of Human Immunoglobulin G3 (IgG3) *
    Article Snippet: .. We therefore attempted to use endoproteinase AspN in order to further digest trypsin-generated O-glycopeptides derived from monoclonal IgG3 from “SCDTPPPCPR” to “SC” and “DTPPPCPR.” However, while the majority of the nonglycosylated tryptic hinge repeat peptides were cleaved by AspN, the O-glycopeptides with the same peptide sequence remained undigested. ..

    Article Title: Improvement of the glycoproteomic toolbox with the discovery of a unique C-terminal cleavage specificity of flavastacin for N-glycosylated asparagine
    Article Snippet: .. Enzymes used for digestion were trypsin (Trypsin Sequencing Grade Modified; V5111) from Promega and endoproteinase AspN (AspN; P8104S) from New England Biolabs (Flavastacin, purified host cell protein, Uniprot Q47899; see the corresponding SDS-gel for purity of AspN (Flavastacin) in Supplementary Figure ). .. All buffer and solutions were prepared with deionized and purified water (dH2 O) using a Milli-Q water purification system (18.2 MΩ · cm−1 at 25°C, total organic carbon of 3 ppb) from Merck Millipore.

    Article Title: Assessing in vivo dynamics of multiple quality attributes from a therapeutic IgG4 monoclonal antibody circulating in cynomolgus monkey
    Article Snippet: .. Reagents Trypsin (sequencing grade, V5111 and V5117) was purchased from Promega (Madison, WI); Lysyl Endopeptidase (Mass Spectrometry Grade, 125–05061) was purchased from Wako Chemicals (Richmond, VA); Pepsin (Porcine stomach mucosa, 02195367) was purchased from MP Biomedicals (Solon, OH); Endoproteinase AspN was purchased from New England BioLabs Inc. (P8104S) and Roche Diagnostics (11054589001). .. Dithiothreitol (DTT, D5545), iodoacetamide (IAA, I6125) and N-ethylmaleimide (NEM, E3876–5G) were purchased from Sigma-Aldrich (St. Louis, MO).

    other:

    Article Title: Molecular Basis of Glucagon-like Peptide 1 Docking to Its Intact Receptor Studied with Carboxyl-terminal Photolabile Probes *
    Article Snippet: Endoproteinase Lys-C (Lys-C) was from Roche Applied Science (Indianapolis, IN) and endoproteinase Asp-N (Asp-N) was from New England Biolabs Inc. (Ipswich, MA).

    Article Title: Refinement of Glucagon-like Peptide 1 Docking to Its Intact Receptor Using Mid-region Photolabile Probes and Molecular Modeling
    Article Snippet: Endoproteinase Glu-C (Glu-C) and endoproteinase Asp-N (Asp-N) were from New England Biolabs Inc. (Ipswich, MA).

    Mass Spectrometry:

    Article Title: Assessing in vivo dynamics of multiple quality attributes from a therapeutic IgG4 monoclonal antibody circulating in cynomolgus monkey
    Article Snippet: .. Reagents Trypsin (sequencing grade, V5111 and V5117) was purchased from Promega (Madison, WI); Lysyl Endopeptidase (Mass Spectrometry Grade, 125–05061) was purchased from Wako Chemicals (Richmond, VA); Pepsin (Porcine stomach mucosa, 02195367) was purchased from MP Biomedicals (Solon, OH); Endoproteinase AspN was purchased from New England BioLabs Inc. (P8104S) and Roche Diagnostics (11054589001). .. Dithiothreitol (DTT, D5545), iodoacetamide (IAA, I6125) and N-ethylmaleimide (NEM, E3876–5G) were purchased from Sigma-Aldrich (St. Louis, MO).

    Modification:

    Article Title: Improvement of the glycoproteomic toolbox with the discovery of a unique C-terminal cleavage specificity of flavastacin for N-glycosylated asparagine
    Article Snippet: .. Enzymes used for digestion were trypsin (Trypsin Sequencing Grade Modified; V5111) from Promega and endoproteinase AspN (AspN; P8104S) from New England Biolabs (Flavastacin, purified host cell protein, Uniprot Q47899; see the corresponding SDS-gel for purity of AspN (Flavastacin) in Supplementary Figure ). .. All buffer and solutions were prepared with deionized and purified water (dH2 O) using a Milli-Q water purification system (18.2 MΩ · cm−1 at 25°C, total organic carbon of 3 ppb) from Merck Millipore.

    Derivative Assay:

    Article Title: Hinge-Region O-Glycosylation of Human Immunoglobulin G3 (IgG3) *
    Article Snippet: .. We therefore attempted to use endoproteinase AspN in order to further digest trypsin-generated O-glycopeptides derived from monoclonal IgG3 from “SCDTPPPCPR” to “SC” and “DTPPPCPR.” However, while the majority of the nonglycosylated tryptic hinge repeat peptides were cleaved by AspN, the O-glycopeptides with the same peptide sequence remained undigested. ..

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  • 92
    New England Biolabs endoproteinase asp n asp n
    <t>Endoproteinase</t> <t>Asp-N</t> cleavage of the GLP1 receptor labeled with the Bpa 16 probe. Shown at the left is a diagram of the predicted sites of Asp-N cleavage of the GLP1 receptor along with the masses. Shown at the right is a representative autoradiograph
    Endoproteinase Asp N Asp N, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/endoproteinase asp n asp n/product/New England Biolabs
    Average 92 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    endoproteinase asp n asp n - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    Image Search Results


    Endoproteinase Asp-N cleavage of the GLP1 receptor labeled with the Bpa 16 probe. Shown at the left is a diagram of the predicted sites of Asp-N cleavage of the GLP1 receptor along with the masses. Shown at the right is a representative autoradiograph

    Journal: The Journal of Biological Chemistry

    Article Title: Refinement of Glucagon-like Peptide 1 Docking to Its Intact Receptor Using Mid-region Photolabile Probes and Molecular Modeling

    doi: 10.1074/jbc.M110.217901

    Figure Lengend Snippet: Endoproteinase Asp-N cleavage of the GLP1 receptor labeled with the Bpa 16 probe. Shown at the left is a diagram of the predicted sites of Asp-N cleavage of the GLP1 receptor along with the masses. Shown at the right is a representative autoradiograph

    Article Snippet: Endoproteinase Glu-C (Glu-C) and endoproteinase Asp-N (Asp-N) were from New England Biolabs Inc. (Ipswich, MA).

    Techniques: Labeling, Autoradiography

    Endoproteinase Asp-N cleavage of the GLP1 receptor labeled with the Bpa 20 probe. Shown at the left is a diagram of the predicted sites of Asp-N cleavage of the GLP1 receptor along with the masses. Shown at the right is a representative autoradiograph

    Journal: The Journal of Biological Chemistry

    Article Title: Refinement of Glucagon-like Peptide 1 Docking to Its Intact Receptor Using Mid-region Photolabile Probes and Molecular Modeling

    doi: 10.1074/jbc.M110.217901

    Figure Lengend Snippet: Endoproteinase Asp-N cleavage of the GLP1 receptor labeled with the Bpa 20 probe. Shown at the left is a diagram of the predicted sites of Asp-N cleavage of the GLP1 receptor along with the masses. Shown at the right is a representative autoradiograph

    Article Snippet: Endoproteinase Glu-C (Glu-C) and endoproteinase Asp-N (Asp-N) were from New England Biolabs Inc. (Ipswich, MA).

    Techniques: Labeling, Autoradiography

    Further localization of the domain of Bpa 35 probe labeling. Left shows that endoproteinase Asp-N cleavage of the M r = 15,000 fragment resulting from Lys-C cleavage of the wild-type receptor yielded a band migrating at approximate M r = 4,000. Right shows

    Journal:

    Article Title: Molecular Basis of Glucagon-like Peptide 1 Docking to Its Intact Receptor Studied with Carboxyl-terminal Photolabile Probes *

    doi: 10.1074/jbc.M109.038109

    Figure Lengend Snippet: Further localization of the domain of Bpa 35 probe labeling. Left shows that endoproteinase Asp-N cleavage of the M r = 15,000 fragment resulting from Lys-C cleavage of the wild-type receptor yielded a band migrating at approximate M r = 4,000. Right shows

    Article Snippet: Endoproteinase Lys-C (Lys-C) was from Roche Applied Science (Indianapolis, IN) and endoproteinase Asp-N (Asp-N) was from New England Biolabs Inc. (Ipswich, MA).

    Techniques: Labeling