trypsin (New England Biolabs)

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Name:

Trypsin ultra Mass Spectrometry Grade

Description:

Trypsin ultra Mass Spectrometry Grade 100 ug

Catalog Number:

p8101s

Price:

Size:

100 ug

Category:

Proteases

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Structured Review

New England Biolabs trypsin

Trypsin ultra Mass Spectrometry Grade 100 ug
https://www.bioz.com/result/trypsin/product/New England Biolabs
Average 94 stars, based on 3 article reviews
Price from $9.99 to $1999.99

trypsin - by Bioz Stars, 2020-02

94/100 stars

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Images

High Performance Liquid Chromatography:

Article Title: The intrinsically disordered linker of E. coliSSB is critical for the release from single‐stranded DNA
Article Snippet: Trypsin‐ultra (Mass Spectrometry Grade), Φx174 Virion DNA, Φx174 RFI DNA and restriction enzymes were purchased from New England BioLab (MA, USA). .. Primers and HPLC purified 5′ATTO488(dT)65 were purchased from Integrated DNA Technologies (IA, USA).

Flow Cytometry:

Article Title: Effects of Covalent Modification by 4-hydroxy-2-nonenal on the Noncovalent Oligomerization of Ubiquitin
Article Snippet: Prior to use, ethanol in the HNE or HHE stock solution was removed by argon flow and HNE or HHE was solubilized in the buffer. .. Trypsin Ultra-Mass Spectrometric Grade in trypsin buffer (50 mM Tris-HCl, 20mM CaCl2 , pH 8.0), was purchased from Biolab, New England.

Article Title: The intrinsically disordered linker of E. coliSSB is critical for the release from single‐stranded DNA
Article Snippet: HisTrap FF crude columns, HiPrep DEAE FF 16/10, Q Sepharose Fast Flow, Mono Q HR 10/10, HiPrep SP Sepharose FF 16/10, HiPrep Heparin FF 16/10, MonoS 5/50 GL, and adenosine triphosphate (ATP) were from GE Healthcare Life Sciences (NJ, USA). .. Trypsin‐ultra (Mass Spectrometry Grade), Φx174 Virion DNA, Φx174 RFI DNA and restriction enzymes were purchased from New England BioLab (MA, USA).

Positron Emission Tomography:

Article Title: Refinement of immunizing antigens to produce functional blocking antibodies against the AniA nitrite reductase of Neisseria gonorrhoeae
Article Snippet: Trypsin digest of surface exposed proteins pET-BL21 and AniA-BL21 cells were inoculated into LB broth supplemented with ampicillin at a concentration of 100μg ml-1 from a mid-log phase culture (OD600nm = ~ 0.4) and grown at 37°C for 1 hour before protein expression was induced with 0.1mM IPTG at 22°C for 3 hours. .. 100μl of cell suspension was combined with 0μg or 2μg of trypsin (Mass Spectrometry Grade, New England Biolabs) and incubated at 37°C for 60 mins.

Sample Prep:

Article Title: Tissue-specific transcriptomics and proteomics of a filarial nematode and its Wolbachia endosymbiont
Article Snippet: Samples (100 μL cell lysate) were then prepared for 2D-LC-MS/MS based proteome analysis using the filter aided sample preparation (FASP) kits (Expedion) with modifications to the protocol for simplification (for a complete protocol see [ ]). .. Samples were digested on-filter with 10 μg of Trypsin-ultra Mass Spectrometry Grade (New England Biolabs) overnight at 37 °C.

Article Title: Proteomic identification of mammalian cell surface derived glycosylphosphatidylinositol-anchored proteins through selective glycan enrichment
Article Snippet: 2.7 2D LC-MS/MS Bead-based samples were digested overnight with 50 ng/μL Trypsin Ultra Mass Spectrometry Grade (New England Biolabs) at 37°C. .. After sample preparation, individual aliquots of the complex peptide mixture were loaded onto a split phase 2D RP-strong cation exchange (SCX) back column.

Expressing:

Modification:

Article Title: Effects of Covalent Modification by 4-hydroxy-2-nonenal on the Noncovalent Oligomerization of Ubiquitin
Article Snippet: In order to monitor the HNE modification to unfolded Ub, Guanidine-HCl solution was added (final concentration 6M) to the Ub solution prior trypsin digestion. .. Trypsin Ultra-Mass Spectrometric Grade in trypsin buffer (50 mM Tris-HCl, 20mM CaCl2 , pH 8.0), was purchased from Biolab, New England.

Synthesized:

Article Title: Autophagy impairment mediated by S-nitrosation of ATG4B leads to neurotoxicity in response to hyperglycemia
Article Snippet: ATP (P0756S), apyrase (M0393S), trypsin (P8101S), GluC (P8100S) and the restriction endonucleases were purchased from New England Biolabs. .. S-nitrosoglutathione (GSNO) and S-nitrosocysteine (SNOC) were synthesized from glutathione using acidified nitrite.

Article Title: Evaluation of a glycoengineered monoclonal antibody via LC-MS analysis in combination with multiple enzymatic digestion
Article Snippet: Sialoglycan oxazoline was synthesized following a previously reported procedure. .. Trypsin-ultra (P8101S), PNGase F (P0708S), α2-3 Neuraminidase S (P0743S), α2-3,6,8,9 Neuraminidase A (P0722S), Remove-iT Endo S (P0741S) are products from New England Biolabs.

Isolation:

Article Title: Tissue-specific transcriptomics and proteomics of a filarial nematode and its Wolbachia endosymbiont
Article Snippet: Paragraph title: Protein isolation and ms-based proteomic analysis ... Samples were digested on-filter with 10 μg of Trypsin-ultra Mass Spectrometry Grade (New England Biolabs) overnight at 37 °C.

Incubation:

Article Title: Refinement of immunizing antigens to produce functional blocking antibodies against the AniA nitrite reductase of Neisseria gonorrhoeae
Article Snippet: .. 100μl of cell suspension was combined with 0μg or 2μg of trypsin (Mass Spectrometry Grade, New England Biolabs) and incubated at 37°C for 60 mins. ..

Article Title: Effects of Covalent Modification by 4-hydroxy-2-nonenal on the Noncovalent Oligomerization of Ubiquitin
Article Snippet: Trypsin Ultra-Mass Spectrometric Grade in trypsin buffer (50 mM Tris-HCl, 20mM CaCl2 , pH 8.0), was purchased from Biolab, New England. .. After collecting the first aliquot (10 µl) of the sample (time 0), it was incubated at 37°C.

Article Title: Comparative Evaluation of the Antimicrobial Activity of Different Antimicrobial Peptides against a Range of Pathogenic Bacteria
Article Snippet: .. The effect of proteases, including trypsin (trypsin ultra, NEB, P8101) and proteinase K (NEB, P8107S), on antimicrobial activity of selected AMPs was investigated by incubation with the respective protease at 37°C for either 30 seconds, 2, 5, 15 or 30 minutes. .. The protease to AMP ratio used in the assay was 1:100 (w/w) and the buffers used were trypsin-ultra reaction buffer (NEB, P8101) for trypsin and 100 mM Tris-HCl pH 7.5 for proteinase K digestion.

Article Title: Tissue-specific transcriptomics and proteomics of a filarial nematode and its Wolbachia endosymbiont
Article Snippet: Protein isolation and ms-based proteomic analysis D. immitis tissue samples were lysed in 1 % SDS by sonication (3 times for 30 s) followed by incubation at 65 °C for 30 min with gentle rocking. .. Samples were digested on-filter with 10 μg of Trypsin-ultra Mass Spectrometry Grade (New England Biolabs) overnight at 37 °C.

Article Title: Comparative Evaluation of the Antimicrobial Activity of Different Antimicrobial Peptides against a Range of Pathogenic Bacteria
Article Snippet: The effect of proteases, including trypsin (trypsin ultra, NEB, P8101) and proteinase K (NEB, P8107S), on antimicrobial activity of selected AMPs was investigated by incubation with the respective protease at 37°C for either 30 seconds, 2, 5, 15 or 30 minutes. .. The protease to AMP ratio used in the assay was 1:100 (w/w) and the buffers used were trypsin-ultra reaction buffer (NEB, P8101) for trypsin and 100 mM Tris-HCl pH 7.5 for proteinase K digestion.

Purification:

Concentration Assay:

Article Title: Comparative Evaluation of the Antimicrobial Activity of Different Antimicrobial Peptides against a Range of Pathogenic Bacteria
Article Snippet: The effect of proteases, including trypsin (trypsin ultra, NEB, P8101) and proteinase K (NEB, P8107S), on antimicrobial activity of selected AMPs was investigated by incubation with the respective protease at 37°C for either 30 seconds, 2, 5, 15 or 30 minutes. .. Afterwards, the antimicrobial activity was determined by measuring the minimal inhibitory concentration (see )).

Two Tailed Test:

Article Title: Refinement of immunizing antigens to produce functional blocking antibodies against the AniA nitrite reductase of Neisseria gonorrhoeae
Article Snippet: 100μl of cell suspension was combined with 0μg or 2μg of trypsin (Mass Spectrometry Grade, New England Biolabs) and incubated at 37°C for 60 mins. .. Differences between CFUs/ml at t0 and at t60 for each sample were assessed for statistical significance with Prism 5 software (GraphPad Software, Inc.) using a two-tailed unpaired Student’s t-test .

Activity Assay:

Article Title: Comparative Evaluation of the Antimicrobial Activity of Different Antimicrobial Peptides against a Range of Pathogenic Bacteria
Article Snippet: Paragraph title: Effect of temperature and proteases on antimicrobial activity ... The protease to AMP ratio used in the assay was 1:100 (w/w) and the buffers used were trypsin-ultra reaction buffer (NEB, P8101) for trypsin and 100 mM Tris-HCl pH 7.5 for proteinase K digestion.

Mass Spectrometry:

Article Title: Refinement of immunizing antigens to produce functional blocking antibodies against the AniA nitrite reductase of Neisseria gonorrhoeae
Article Snippet: .. 100μl of cell suspension was combined with 0μg or 2μg of trypsin (Mass Spectrometry Grade, New England Biolabs) and incubated at 37°C for 60 mins. ..

Article Title: Tissue-specific transcriptomics and proteomics of a filarial nematode and its Wolbachia endosymbiont
Article Snippet: .. Samples were digested on-filter with 10 μg of Trypsin-ultra Mass Spectrometry Grade (New England Biolabs) overnight at 37 °C. .. Digested samples were eluted into a clean collection tube via three steps of centrifugation at 14,000 × g for 15 min as follows: Step 1: 80 μL 50 mM ammonium bicarbonate solution; Step 2: 50 μL 0.5 M sodium chloride solution (provided with the FASP kit); Step 3: 170 μL H2 O with 0.1 % formic acid.

Article Title: Proteomic identification of mammalian cell surface derived glycosylphosphatidylinositol-anchored proteins through selective glycan enrichment
Article Snippet: .. 2.7 2D LC-MS/MS Bead-based samples were digested overnight with 50 ng/μL Trypsin Ultra Mass Spectrometry Grade (New England Biolabs) at 37°C. .. After sample preparation, individual aliquots of the complex peptide mixture were loaded onto a split phase 2D RP-strong cation exchange (SCX) back column.

Article Title: The intrinsically disordered linker of E. coliSSB is critical for the release from single‐stranded DNA
Article Snippet: .. Trypsin‐ultra (Mass Spectrometry Grade), Φx174 Virion DNA, Φx174 RFI DNA and restriction enzymes were purchased from New England BioLab (MA, USA). .. Primers and HPLC purified 5′ATTO488(dT)65 were purchased from Integrated DNA Technologies (IA, USA).

Lysis:

Article Title: Refinement of immunizing antigens to produce functional blocking antibodies against the AniA nitrite reductase of Neisseria gonorrhoeae
Article Snippet: 100μl of cell suspension was combined with 0μg or 2μg of trypsin (Mass Spectrometry Grade, New England Biolabs) and incubated at 37°C for 60 mins. .. This was conducted to ensure that cell lysis was not occurring over the course of the assay, which would be indicated by a reduction in CFUs/ml.

IA:

Sonication:

Centrifugation:

Article Title: Tissue-specific transcriptomics and proteomics of a filarial nematode and its Wolbachia endosymbiont
Article Snippet: Samples were digested on-filter with 10 μg of Trypsin-ultra Mass Spectrometry Grade (New England Biolabs) overnight at 37 °C. .. Digested samples were eluted into a clean collection tube via three steps of centrifugation at 14,000 × g for 15 min as follows: Step 1: 80 μL 50 mM ammonium bicarbonate solution; Step 2: 50 μL 0.5 M sodium chloride solution (provided with the FASP kit); Step 3: 170 μL H2 O with 0.1 % formic acid.

T-Test:

Article Title: Refinement of immunizing antigens to produce functional blocking antibodies against the AniA nitrite reductase of Neisseria gonorrhoeae
Article Snippet: 100μl of cell suspension was combined with 0μg or 2μg of trypsin (Mass Spectrometry Grade, New England Biolabs) and incubated at 37°C for 60 mins. .. Differences between CFUs/ml at t0 and at t60 for each sample were assessed for statistical significance with Prism 5 software (GraphPad Software, Inc.) using a two-tailed unpaired Student’s t-test .

Injection:

Article Title: Proteomic identification of mammalian cell surface derived glycosylphosphatidylinositol-anchored proteins through selective glycan enrichment
Article Snippet: 2.7 2D LC-MS/MS Bead-based samples were digested overnight with 50 ng/μL Trypsin Ultra Mass Spectrometry Grade (New England Biolabs) at 37°C. .. Column was packed using a PicoView Pressure Injection Cell (New Objective).

Software:

Article Title: Refinement of immunizing antigens to produce functional blocking antibodies against the AniA nitrite reductase of Neisseria gonorrhoeae
Article Snippet: 100μl of cell suspension was combined with 0μg or 2μg of trypsin (Mass Spectrometry Grade, New England Biolabs) and incubated at 37°C for 60 mins. .. Differences between CFUs/ml at t0 and at t60 for each sample were assessed for statistical significance with Prism 5 software (GraphPad Software, Inc.) using a two-tailed unpaired Student’s t-test .