trypsin ultratm (New England Biolabs)

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Name:

Trypsin ultra Mass Spectrometry Grade

Description:

Trypsin ultra Mass Spectrometry Grade 100 ug

Catalog Number:

p8101s

Price:

Size:

100 ug

Category:

Proteases

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Structured Review

New England Biolabs trypsin ultratm

Trypsin ultra Mass Spectrometry Grade 100 ug
https://www.bioz.com/result/trypsin ultratm/product/New England Biolabs
Average 94 stars, based on 42 article reviews
Price from $9.99 to $1999.99

trypsin ultratm - by Bioz Stars, 2020-08

94/100 stars

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Incubation:

Article Title: Structural insights into the mechanism of oxidative activation of heme-free H-NOX from Vibrio cholerae
Article Snippet: .. Trypsin-ultraTM (New England Biolabs) was added at a mass ratio of 1 : 50 and incubated at 37°C overnight. ..

Article Title: Comparative Evaluation of the Antimicrobial Activity of Different Antimicrobial Peptides against a Range of Pathogenic Bacteria
Article Snippet: .. The effect of proteases, including trypsin (trypsin ultra, NEB, P8101) and proteinase K (NEB, P8107S), on antimicrobial activity of selected AMPs was investigated by incubation with the respective protease at 37°C for either 30 seconds, 2, 5, 15 or 30 minutes. .. The protease to AMP ratio used in the assay was 1:100 (w/w) and the buffers used were trypsin-ultra reaction buffer (NEB, P8101) for trypsin and 100 mM Tris-HCl pH 7.5 for proteinase K digestion.

Activity Assay:

Mass Spectrometry:

Article Title: Leucine 232 and hydrophobic residues at the ribosomal P stalk binding site are critical for biological activity of ricin
Article Snippet: .. Protease digestion GluC and TPCK treated trypsin (Trypsin-ultra, MS grade) were purchased from New England Biolabs (Ipswich, MA, U.S.A.). .. Four microgram of protein was incubated with 0.2 μg of Glu-C (1:20) in Glu-C reaction buffer (50 mM Tris/HCl pH 8, 0.5 mM Glu–Glu dipeptide) for 4 h at 37°C.

Article Title: Tissue-specific transcriptomics and proteomics of a filarial nematode and its Wolbachia endosymbiont
Article Snippet: .. Samples were digested on-filter with 10 μg of Trypsin-ultra Mass Spectrometry Grade (New England Biolabs) overnight at 37 °C. .. Digested samples were eluted into a clean collection tube via three steps of centrifugation at 14,000 × g for 15 min as follows: Step 1: 80 μL 50 mM ammonium bicarbonate solution; Step 2: 50 μL 0.5 M sodium chloride solution (provided with the FASP kit); Step 3: 170 μL H2 O with 0.1 % formic acid.

Article Title: The intrinsically disordered linker of E. coliSSB is critical for the release from single‐stranded DNA
Article Snippet: .. Trypsin‐ultra (Mass Spectrometry Grade), Φx174 Virion DNA, Φx174 RFI DNA and restriction enzymes were purchased from New England BioLab (MA, USA). .. Primers and HPLC purified 5′ATTO488(dT)65 were purchased from Integrated DNA Technologies (IA, USA).