trypsin  (New England Biolabs)


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  • 95
    Name:
    Trypsin ultra Mass Spectrometry Grade
    Description:
    Trypsin ultra Mass Spectrometry Grade 100 ug
    Catalog Number:
    P8101S
    Price:
    82
    Category:
    Proteases
    Size:
    100 ug
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    Structured Review

    New England Biolabs trypsin
    Trypsin ultra Mass Spectrometry Grade
    Trypsin ultra Mass Spectrometry Grade 100 ug
    https://www.bioz.com/result/trypsin/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    trypsin - by Bioz Stars, 2021-07
    95/100 stars

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    Related Articles

    Mass Spectrometry:

    Article Title: Computational and experimental analysis of the glycophosphatidylinositol-anchored proteome of the human parasitic nematode Brugia malayi
    Article Snippet: Each sample was analyzed using an automated 3-step MudPIT protocol on a Q Exactive mass spectrometer (ThermoFisher Scientific, Waltham MA) using HCD fragmentation. .. Membrane samplesMembrane samples were digested with Trypsin-ultra, Mass Spectrometry Grade (New England Biolabs) upon addition of ProteaseMax (Promega, Madison WI) for 3 hours at 37°C. .. Each sample was analyzed using an 8-step MudPIT protocol on an LTQ-Orbitrap XL (ThermoFisher Scientific) using CID fragmentation.

    Article Title: Refinement of immunizing antigens to produce functional blocking antibodies against the AniA nitrite reductase of Neisseria gonorrhoeae
    Article Snippet: .. 100μl of cell suspension was combined with 0μg or 2μg of trypsin (Mass Spectrometry Grade, New England Biolabs) and incubated at 37°C for 60 mins. ..

    Article Title: Tissue-specific transcriptomics and proteomics of a filarial nematode and its Wolbachia endosymbiont
    Article Snippet: Samples (100 μL cell lysate) were then prepared for 2D-LC-MS/MS based proteome analysis using the filter aided sample preparation (FASP) kits (Expedion) with modifications to the protocol for simplification (for a complete protocol see [ ]). .. Samples were digested on-filter with 10 μg of Trypsin-ultra Mass Spectrometry Grade (New England Biolabs) overnight at 37 °C. .. Digested samples were eluted into a clean collection tube via three steps of centrifugation at 14,000 × g for 15 min as follows: Step 1: 80 μL 50 mM ammonium bicarbonate solution; Step 2: 50 μL 0.5 M sodium chloride solution (provided with the FASP kit); Step 3: 170 μL H2 O with 0.1 % formic acid.

    Article Title: Proteomic identification of mammalian cell surface derived glycosylphosphatidylinositol-anchored proteins through selective glycan enrichment
    Article Snippet: .. 2.7 2D LC-MS/MS Bead-based samples were digested overnight with 50 ng/μL Trypsin Ultra Mass Spectrometry Grade (New England Biolabs) at 37°C. .. After sample preparation, individual aliquots of the complex peptide mixture were loaded onto a split phase 2D RP-strong cation exchange (SCX) back column.

    Article Title: Computational and experimental analysis of the glycophosphatidylinositol-anchored proteome of the human parasitic nematode Brugia malayi
    Article Snippet: Surface samples Surface samples were digested with Trypsin-ultra, Mass Spectrometry Grade (New England Biolabs, Ipswich MA) overnight at 37°C using a filter aided sample preparation kit (Expedeon, San Diego CA). .. Surface samplesSurface samples were digested with Trypsin-ultra, Mass Spectrometry Grade (New England Biolabs, Ipswich MA) overnight at 37°C using a filter aided sample preparation kit (Expedeon, San Diego CA). .. Each sample was analyzed using an automated 3-step MudPIT protocol on a Q Exactive mass spectrometer (ThermoFisher Scientific, Waltham MA) using HCD fragmentation.

    Incubation:

    Article Title: Refinement of immunizing antigens to produce functional blocking antibodies against the AniA nitrite reductase of Neisseria gonorrhoeae
    Article Snippet: .. 100μl of cell suspension was combined with 0μg or 2μg of trypsin (Mass Spectrometry Grade, New England Biolabs) and incubated at 37°C for 60 mins. ..

    Sample Prep:

    Article Title: Computational and experimental analysis of the glycophosphatidylinositol-anchored proteome of the human parasitic nematode Brugia malayi
    Article Snippet: Surface samples Surface samples were digested with Trypsin-ultra, Mass Spectrometry Grade (New England Biolabs, Ipswich MA) overnight at 37°C using a filter aided sample preparation kit (Expedeon, San Diego CA). .. Surface samplesSurface samples were digested with Trypsin-ultra, Mass Spectrometry Grade (New England Biolabs, Ipswich MA) overnight at 37°C using a filter aided sample preparation kit (Expedeon, San Diego CA). .. Each sample was analyzed using an automated 3-step MudPIT protocol on a Q Exactive mass spectrometer (ThermoFisher Scientific, Waltham MA) using HCD fragmentation.

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  • 95
    New England Biolabs mass spectrometry grade
    Mass Spectrometry Grade, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mass spectrometry grade/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mass spectrometry grade - by Bioz Stars, 2021-07
    95/100 stars
      Buy from Supplier

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