endoproteinase gluc  (New England Biolabs)


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  • 93
    Name:
    Endoproteinase GluC
    Description:
    Endoproteinase GluC 50 ug
    Catalog Number:
    p8100s
    Price:
    81
    Size:
    50 ug
    Category:
    Proteases
    Buy from Supplier


    Structured Review

    New England Biolabs endoproteinase gluc
    Endoproteinase GluC
    Endoproteinase GluC 50 ug
    https://www.bioz.com/result/endoproteinase gluc/product/New England Biolabs
    Average 93 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    endoproteinase gluc - by Bioz Stars, 2020-05
    93/100 stars

    Images

    1) Product Images from "Completely monodisperse, highly repetitive proteins for bioconjugate capillary electrophoresis: Development and characterization"

    Article Title: Completely monodisperse, highly repetitive proteins for bioconjugate capillary electrophoresis: Development and characterization

    Journal: Biomacromolecules

    doi: 10.1021/bm200358r

    Western blot of Endoproteinase GluC digestion of PC-36 protein over 16 hours A) PC-36 no protease; 2, 4, 8, 16 hr after 1:100 μg protease: μg protein added B) 2, 4, 8, 16 hrs after 1:50 μg protease: μg protein added C)
    Figure Legend Snippet: Western blot of Endoproteinase GluC digestion of PC-36 protein over 16 hours A) PC-36 no protease; 2, 4, 8, 16 hr after 1:100 μg protease: μg protein added B) 2, 4, 8, 16 hrs after 1:50 μg protease: μg protein added C)

    Techniques Used: Western Blot

    Overlay of MALDI-TOF results of PC-36 before and after digestion by endoproteinase GluC
    Figure Legend Snippet: Overlay of MALDI-TOF results of PC-36 before and after digestion by endoproteinase GluC

    Techniques Used:

    2) Product Images from "Completely monodisperse, highly repetitive proteins for bioconjugate capillary electrophoresis: Development and characterization"

    Article Title: Completely monodisperse, highly repetitive proteins for bioconjugate capillary electrophoresis: Development and characterization

    Journal: Biomacromolecules

    doi: 10.1021/bm200358r

    Western blot of Endoproteinase GluC digestion of PC-36 protein over 16 hours A) PC-36 no protease; 2, 4, 8, 16 hr after 1:100 μg protease: μg protein added B) 2, 4, 8, 16 hrs after 1:50 μg protease: μg protein added C)
    Figure Legend Snippet: Western blot of Endoproteinase GluC digestion of PC-36 protein over 16 hours A) PC-36 no protease; 2, 4, 8, 16 hr after 1:100 μg protease: μg protein added B) 2, 4, 8, 16 hrs after 1:50 μg protease: μg protein added C)

    Techniques Used: Western Blot

    Overlay of MALDI-TOF results of PC-36 before and after digestion by endoproteinase GluC
    Figure Legend Snippet: Overlay of MALDI-TOF results of PC-36 before and after digestion by endoproteinase GluC

    Techniques Used:

    3) Product Images from "Completely monodisperse, highly repetitive proteins for bioconjugate capillary electrophoresis: Development and characterization"

    Article Title: Completely monodisperse, highly repetitive proteins for bioconjugate capillary electrophoresis: Development and characterization

    Journal: Biomacromolecules

    doi: 10.1021/bm200358r

    Western blot of Endoproteinase GluC digestion of PC-36 protein over 16 hours A) PC-36 no protease; 2, 4, 8, 16 hr after 1:100 μg protease: μg protein added B) 2, 4, 8, 16 hrs after 1:50 μg protease: μg protein added C)
    Figure Legend Snippet: Western blot of Endoproteinase GluC digestion of PC-36 protein over 16 hours A) PC-36 no protease; 2, 4, 8, 16 hr after 1:100 μg protease: μg protein added B) 2, 4, 8, 16 hrs after 1:50 μg protease: μg protein added C)

    Techniques Used: Western Blot

    Overlay of MALDI-TOF results of PC-36 before and after digestion by endoproteinase GluC
    Figure Legend Snippet: Overlay of MALDI-TOF results of PC-36 before and after digestion by endoproteinase GluC

    Techniques Used:

    4) Product Images from "Completely monodisperse, highly repetitive proteins for bioconjugate capillary electrophoresis: Development and characterization"

    Article Title: Completely monodisperse, highly repetitive proteins for bioconjugate capillary electrophoresis: Development and characterization

    Journal: Biomacromolecules

    doi: 10.1021/bm200358r

    Western blot of Endoproteinase GluC digestion of PC-36 protein over 16 hours A) PC-36 no protease; 2, 4, 8, 16 hr after 1:100 μg protease: μg protein added B) 2, 4, 8, 16 hrs after 1:50 μg protease: μg protein added C)
    Figure Legend Snippet: Western blot of Endoproteinase GluC digestion of PC-36 protein over 16 hours A) PC-36 no protease; 2, 4, 8, 16 hr after 1:100 μg protease: μg protein added B) 2, 4, 8, 16 hrs after 1:50 μg protease: μg protein added C)

    Techniques Used: Western Blot

    Overlay of MALDI-TOF results of PC-36 before and after digestion by endoproteinase GluC
    Figure Legend Snippet: Overlay of MALDI-TOF results of PC-36 before and after digestion by endoproteinase GluC

    Techniques Used:

    5) Product Images from "Completely monodisperse, highly repetitive proteins for bioconjugate capillary electrophoresis: Development and characterization"

    Article Title: Completely monodisperse, highly repetitive proteins for bioconjugate capillary electrophoresis: Development and characterization

    Journal: Biomacromolecules

    doi: 10.1021/bm200358r

    Western blot of Endoproteinase GluC digestion of PC-36 protein over 16 hours A) PC-36 no protease; 2, 4, 8, 16 hr after 1:100 μg protease: μg protein added B) 2, 4, 8, 16 hrs after 1:50 μg protease: μg protein added C)
    Figure Legend Snippet: Western blot of Endoproteinase GluC digestion of PC-36 protein over 16 hours A) PC-36 no protease; 2, 4, 8, 16 hr after 1:100 μg protease: μg protein added B) 2, 4, 8, 16 hrs after 1:50 μg protease: μg protein added C)

    Techniques Used: Western Blot

    Overlay of MALDI-TOF results of PC-36 before and after digestion by endoproteinase GluC
    Figure Legend Snippet: Overlay of MALDI-TOF results of PC-36 before and after digestion by endoproteinase GluC

    Techniques Used:

    6) Product Images from "Refinement of Glucagon-like Peptide 1 Docking to Its Intact Receptor Using Mid-region Photolabile Probes and Molecular Modeling"

    Article Title: Refinement of Glucagon-like Peptide 1 Docking to Its Intact Receptor Using Mid-region Photolabile Probes and Molecular Modeling

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M110.217901

    Endoproteinase Glu-C cleavage of the GLP1 receptor labeled with the Bpa 16 probe. Shown at the left is a diagram of the predicted sites of Glu-C cleavage of the GLP1 receptor along with the masses. Shown at the right is a representative autoradiograph
    Figure Legend Snippet: Endoproteinase Glu-C cleavage of the GLP1 receptor labeled with the Bpa 16 probe. Shown at the left is a diagram of the predicted sites of Glu-C cleavage of the GLP1 receptor along with the masses. Shown at the right is a representative autoradiograph

    Techniques Used: Labeling, Autoradiography

    Endoproteinase Glu-C cleavage of the GLP1 receptor labeled with the Bpa 20 probe. Shown at the left is a diagram of the predicted sites of Glu-C cleavage of the GLP1 receptor along with the masses. Shown at the right is a representative autoradiograph
    Figure Legend Snippet: Endoproteinase Glu-C cleavage of the GLP1 receptor labeled with the Bpa 20 probe. Shown at the left is a diagram of the predicted sites of Glu-C cleavage of the GLP1 receptor along with the masses. Shown at the right is a representative autoradiograph

    Techniques Used: Labeling, Autoradiography

    7) Product Images from "High Divergence of the Precursor Peptides in Combinatorial Lanthipeptide Biosynthesis"

    Article Title: High Divergence of the Precursor Peptides in Combinatorial Lanthipeptide Biosynthesis

    Journal: ACS Chemical Biology

    doi: 10.1021/cb500622c

    Modification of ProcAt.1 by ProcM. (A) MALDI-ToF-MS analysis of ProcAt.1 that was obtained by coexpression with ProcM and treated with endoproteinase Glu-C (trace i) and subsequently derivatized by NEM (trace ii). (B) Sequence of ProcAt.1 modified by ProcM and treated with Glu-C. The ESI-MS/MS fragmentation pattern for the 3-fold dehydrated species is shown (the MS/MS data is presented in Supporting Information Figure 4 ).
    Figure Legend Snippet: Modification of ProcAt.1 by ProcM. (A) MALDI-ToF-MS analysis of ProcAt.1 that was obtained by coexpression with ProcM and treated with endoproteinase Glu-C (trace i) and subsequently derivatized by NEM (trace ii). (B) Sequence of ProcAt.1 modified by ProcM and treated with Glu-C. The ESI-MS/MS fragmentation pattern for the 3-fold dehydrated species is shown (the MS/MS data is presented in Supporting Information Figure 4 ).

    Techniques Used: Modification, Mass Spectrometry, Sequencing

    Coexpression studies of Cys-lacking peptides with LanMs. (A) MALDI-ToF MS analysis of ProcA4.1 that was obtained by coexpression with ProcM. Also shown is the sequence of the ProcA4.1 core (obtained by TEV cleavage of a ProcA4.1 mutant containing an engineered TEV cleavage site just before the predicted core sequence) and the MS/MS fragmentation pattern for the 3-fold dehydrated species. (B) MALDI-ToF MS analysis of NpnA3 that was obtained by coexpression with NpnM in E. coli . Also shown is the sequence of endoproteinase Glu-C cleaved NpnA3 and the MS/MS fragmentation pattern for the 4-fold dehydrated species. (C) MALDI-ToF MS analysis of NpnA6 obtained similarly to that for NpnA3 in panel B. Also presented is the sequence and MS/MS fragmentation pattern for 3-fold dehydrated NpnA6. The MS/MS data for 3-fold dehydrated ProcA4.1, 4-fold dehydrated NpnA3, and 3-fold dehydrated NpnA6 are shown in Supporting Information Figures 24–26 , respectively.
    Figure Legend Snippet: Coexpression studies of Cys-lacking peptides with LanMs. (A) MALDI-ToF MS analysis of ProcA4.1 that was obtained by coexpression with ProcM. Also shown is the sequence of the ProcA4.1 core (obtained by TEV cleavage of a ProcA4.1 mutant containing an engineered TEV cleavage site just before the predicted core sequence) and the MS/MS fragmentation pattern for the 3-fold dehydrated species. (B) MALDI-ToF MS analysis of NpnA3 that was obtained by coexpression with NpnM in E. coli . Also shown is the sequence of endoproteinase Glu-C cleaved NpnA3 and the MS/MS fragmentation pattern for the 4-fold dehydrated species. (C) MALDI-ToF MS analysis of NpnA6 obtained similarly to that for NpnA3 in panel B. Also presented is the sequence and MS/MS fragmentation pattern for 3-fold dehydrated NpnA6. The MS/MS data for 3-fold dehydrated ProcA4.1, 4-fold dehydrated NpnA3, and 3-fold dehydrated NpnA6 are shown in Supporting Information Figures 24–26 , respectively.

    Techniques Used: Mass Spectrometry, Sequencing, Mutagenesis

    Related Articles

    other:

    Article Title: Completely monodisperse, highly repetitive proteins for bioconjugate capillary electrophoresis: Development and characterization
    Article Snippet: A larger scale reaction was performed using 50 μg of endoproteinase GluC and 5 mg of PC-36 for 6 hours at 25°C.

    Article Title: Refinement of Glucagon-like Peptide 1 Docking to Its Intact Receptor Using Mid-region Photolabile Probes and Molecular Modeling
    Article Snippet: Endoproteinase Glu-C (Glu-C) and endoproteinase Asp-N (Asp-N) were from New England Biolabs Inc. (Ipswich, MA).

    Article Title: Completely monodisperse, highly repetitive proteins for bioconjugate capillary electrophoresis: Development and characterization
    Article Snippet: Endoproteinase GluC is a serine protease that can cleave specifically after Glu residues.

    Article Title: Completely monodisperse, highly repetitive proteins for bioconjugate capillary electrophoresis: Development and characterization
    Article Snippet: It is apparent that removal of the affinity tag by endoproteinase GluC has drawbacks when applied to proteins much larger than PC-36 and further studies are needed to identify the cause of and reduce the polydispersity observed in the PC-72 protein.

    Article Title: Completely monodisperse, highly repetitive proteins for bioconjugate capillary electrophoresis: Development and characterization
    Article Snippet: Endoproteinase GluC has a different mass than the PC-36 protein and thus they are easily distinguishable on the blot.

    Purification:

    Article Title: African trypanosomes evade immune clearance by O-glycosylation of the VSG surface coat
    Article Snippet: .. Preparation of O -glycopeptides Purified VSG3 (50 μg in 25 μl 10 mM sodium phosphate, pH 8) was reduced (10 mM DTT, 85 °C, 20 min), S-alkylated (25 mM IAA, 1h, RT in the dark), diluted with an equal volume of 2 x GluC buffer and digested with 1:25 (w/w) endoproteinase GluC (New England BioLabs) for 24 h, 37 °C, with shaking. .. The GluC fragments were separated using NuPAGE 4-12% Bis-Tris protein gels (Invitrogen), stained with Coomassie Brilliant Blue (Thermo Scientific) and the 17 kDa fragment was excised and subjected to in-gel trypsin digestion for LC-MS/MS analysis.

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    New England Biolabs endoproteinase gluc
    Western blot of <t>Endoproteinase</t> <t>GluC</t> digestion of PC-36 protein over 16 hours A) PC-36 no protease; 2, 4, 8, 16 hr after 1:100 μg protease: μg protein added B) 2, 4, 8, 16 hrs after 1:50 μg protease: μg protein added C)
    Endoproteinase Gluc, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/endoproteinase gluc/product/New England Biolabs
    Average 93 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    endoproteinase gluc - by Bioz Stars, 2020-05
    93/100 stars
      Buy from Supplier

    Image Search Results


    Western blot of Endoproteinase GluC digestion of PC-36 protein over 16 hours A) PC-36 no protease; 2, 4, 8, 16 hr after 1:100 μg protease: μg protein added B) 2, 4, 8, 16 hrs after 1:50 μg protease: μg protein added C)

    Journal: Biomacromolecules

    Article Title: Completely monodisperse, highly repetitive proteins for bioconjugate capillary electrophoresis: Development and characterization

    doi: 10.1021/bm200358r

    Figure Lengend Snippet: Western blot of Endoproteinase GluC digestion of PC-36 protein over 16 hours A) PC-36 no protease; 2, 4, 8, 16 hr after 1:100 μg protease: μg protein added B) 2, 4, 8, 16 hrs after 1:50 μg protease: μg protein added C)

    Article Snippet: Endoproteinase GluC is a serine protease that can cleave specifically after Glu residues.

    Techniques: Western Blot

    Overlay of MALDI-TOF results of PC-36 before and after digestion by endoproteinase GluC

    Journal: Biomacromolecules

    Article Title: Completely monodisperse, highly repetitive proteins for bioconjugate capillary electrophoresis: Development and characterization

    doi: 10.1021/bm200358r

    Figure Lengend Snippet: Overlay of MALDI-TOF results of PC-36 before and after digestion by endoproteinase GluC

    Article Snippet: Endoproteinase GluC is a serine protease that can cleave specifically after Glu residues.

    Techniques: