ribonuclease b (New England Biolabs)


Structured Review

Ribonuclease B, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ribonuclease b/product/New England Biolabs
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Direct MALDI Glycotyping of Glycoproteins toward Practical Subtyping of Biological Samples"
Article Title: Direct MALDI Glycotyping of Glycoproteins toward Practical Subtyping of Biological Samples
Journal: ACS Omega
doi: 10.1021/acsomega.2c05429

Figure Legend Snippet: (a) 0,2 A, 2,4 A, and B type ISD fragment position of N -glycans. MALDI–ISD spectrum of RNase B (20 pmol μL –1 ) with (b) 1,5-diaminonaphthalene (DAN)/DHB/Na (12:10:1), (c) DAN/aniline/DHB/Na (2:10:10:1), and (d) DAN/DHB/Na (2:10:1).
Techniques Used:
ribonuclease b (New England Biolabs)


Structured Review

Ribonuclease B, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ribonuclease b/product/New England Biolabs
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Direct MALDI Glycotyping of Glycoproteins toward Practical Subtyping of Biological Samples"
Article Title: Direct MALDI Glycotyping of Glycoproteins toward Practical Subtyping of Biological Samples
Journal: ACS Omega
doi: 10.1021/acsomega.2c05429

Figure Legend Snippet: (a) 0,2 A, 2,4 A, and B type ISD fragment position of N -glycans. MALDI–ISD spectrum of RNase B (20 pmol μL –1 ) with (b) 1,5-diaminonaphthalene (DAN)/DHB/Na (12:10:1), (c) DAN/aniline/DHB/Na (2:10:10:1), and (d) DAN/DHB/Na (2:10:1).
Techniques Used:
rnase b (New England Biolabs)


Structured Review

Rnase B, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rnase b/product/New England Biolabs
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Mechanism of cooperative N -glycan processing by the multi-modular endoglycosidase EndoE"
Article Title: Mechanism of cooperative N -glycan processing by the multi-modular endoglycosidase EndoE
Journal: Nature Communications
doi: 10.1038/s41467-022-28722-w

Figure Legend Snippet: Processing of HM-type N -glycans on RNAse B. Mass spectrometry of RNAse B treated with a no enzyme (negative control) b EndoE-GH18L c EndoE-GH18L E186Q d EndoE-GH18L + EndoE-GH18L E186Q e EndoE-GH20 f EndoE-GH20 E662Q g EndoE-GH20 + EndoE-GH20 E662Q h EndoBT-3987 (positive control) i EndoE j EndoE-GH18L + EndoE-GH20 k EndoE E186Q l EndoE E186Q + EndoE-GH18L m EndoE E662Q n EndoE E662Q + EndoE-GH20. The peaks corresponding to intact RNaseB are numbered based on the glycoforms found in the single glycosylation site of the protein. The retention time for RNAseB was 2.1 min. For mass deconvolution, the following parameters were used in the BioConfirm software; 1000–2400 m/z and 130–160 kDa. The theoretical and observed mass of each annotated peak are in Supplementary Table .
Techniques Used: Mass Spectrometry, Negative Control, Positive Control, Software
rnase b (New England Biolabs)


Structured Review
Rnase B, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rnase b/product/New England Biolabs
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
bovine rnase b (New England Biolabs)


Structured Review

Bovine Rnase B, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bovine rnase b/product/New England Biolabs
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Systems-wide analysis of glycoprotein conformational changes by limited deglycosylation assay"
Article Title: Systems-wide analysis of glycoprotein conformational changes by limited deglycosylation assay
Journal: bioRxiv
doi: 10.1101/2021.06.04.447131

Figure Legend Snippet: Experimental conditions for optimal limited deglycosylation of bovine RNase B and fetuin by PNGase F were determined. The optimal A ) NP-40 concentration, B ) temperature, C ) and D ) PNGase F:glycoprotein ratio for RNase B and fetuin, respectively, and, E ) and F ) the optimal time for the deglycosylation of RNase B and fetuin, respectively, were assayed by differential migration of the glycoproteins in 15% SDS-PAGE. Fully deglycosylated (+) and fully glycosylated (−) proteins were included for each optimization reaction, denoted by FD and FG, respectively.
Techniques Used: Concentration Assay, Migration, SDS Page
rnase b (New England Biolabs)


Structured Review

Rnase B, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rnase b/product/New England Biolabs
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Systems-wide analysis of glycoprotein conformational changes by limited deglycosylation assay"
Article Title: Systems-wide analysis of glycoprotein conformational changes by limited deglycosylation assay
Journal: bioRxiv
doi: 10.1101/2021.06.04.447131

Figure Legend Snippet: Experimental conditions for optimal limited deglycosylation of bovine RNase B and fetuin by PNGase F were determined. The optimal A ) NP-40 concentration, B ) temperature, C ) and D ) PNGase F:glycoprotein ratio for RNase B and fetuin, respectively, and, E ) and F ) the optimal time for the deglycosylation of RNase B and fetuin, respectively, were assayed by differential migration of the glycoproteins in 15% SDS-PAGE. Fully deglycosylated (+) and fully glycosylated (−) proteins were included for each optimization reaction, denoted by FD and FG, respectively.
Techniques Used: Concentration Assay, Migration, SDS Page
ribonuclease b (New England Biolabs)


Structured Review
Ribonuclease B, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ribonuclease b/product/New England Biolabs
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
rnase b (New England Biolabs)


Structured Review
Rnase B, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rnase b/product/New England Biolabs
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
rnase b (New England Biolabs)


Structured Review
Rnase B, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rnase b/product/New England Biolabs
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
rnase b (New England Biolabs)


Structured Review

Rnase B, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rnase b/product/New England Biolabs
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "New Enzymatic Approach to Distinguish Fucosylation Isomers of N‑Linked Glycans in Tissues Using MALDI Imaging Mass Spectrometry"
Article Title: New Enzymatic Approach to Distinguish Fucosylation Isomers of N‑Linked Glycans in Tissues Using MALDI Imaging Mass Spectrometry
Journal: Journal of proteome research
doi: 10.1021/acs.jproteome.0c00024

Figure Legend Snippet: SDS-PAGE analysis of N-linked glycans cleaved by PNGase F or Endo F3. (A) Cartoon description of Endo F3 vs PNGase F cleavage on core-fucosylated N-linked glycans. For glycans, red triangles represent fucose, blue squares represent N-acetylglucosamine, green circles represent mannose, and yellow circles represent galactose. (B) SDS-PAGE analysis of Endo F3 and PNGase F digestion of human Fetuin-A or RNase B
Techniques Used: SDS Page
rnase b (New England Biolabs)


Structured Review

Rnase B, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rnase b/product/New England Biolabs
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "New Enzymatic Approach to Distinguish Fucosylation Isomers of N‑Linked Glycans in Tissues Using MALDI Imaging Mass Spectrometry"
Article Title: New Enzymatic Approach to Distinguish Fucosylation Isomers of N‑Linked Glycans in Tissues Using MALDI Imaging Mass Spectrometry
Journal: Journal of proteome research
doi: 10.1021/acs.jproteome.0c00024

Figure Legend Snippet: SDS-PAGE analysis of N-linked glycans cleaved by PNGase F or Endo F3. (A) Cartoon description of Endo F3 vs PNGase F cleavage on core-fucosylated N-linked glycans. For glycans, red triangles represent fucose, blue squares represent N-acetylglucosamine, green circles represent mannose, and yellow circles represent galactose. (B) SDS-PAGE analysis of Endo F3 and PNGase F digestion of human Fetuin-A or RNase B
Techniques Used: SDS Page