color prestained protein standard broad range 10 250 kda 150 gel lanes  (New England Biolabs)


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  • 99
    Name:
    Color Prestained Protein Standard Broad Range 11 245 kDa
    Description:
    Color Prestained Protein Standard Broad Range 11 245 kDa 750 mini gel lanes
    Catalog Number:
    p7712l
    Price:
    684
    Size:
    750 mini gel lanes
    Category:
    Protein Molecular Weight Markers
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    Structured Review

    New England Biolabs color prestained protein standard broad range 10 250 kda 150 gel lanes
    Color Prestained Protein Standard Broad Range 11 245 kDa
    Color Prestained Protein Standard Broad Range 11 245 kDa 750 mini gel lanes
    https://www.bioz.com/result/color prestained protein standard broad range 10 250 kda 150 gel lanes/product/New England Biolabs
    Average 99 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    color prestained protein standard broad range 10 250 kda 150 gel lanes - by Bioz Stars, 2020-09
    99/100 stars

    Images

    Related Articles

    Staining:

    Article Title: Generation of an artificial human B cell line test system using Transpo-mAbTM technology to evaluate the therapeutic efficacy of novel antigen-specific fusion proteins
    Article Snippet: .. Purified proteins were separated by SDS-PAGE and visualized by Coomassie Brilliant Blue staining against Color Prestained Protein Standards, Broad Range (New England Biolabs, Ipswich, Massachusetts). .. Western blot analysis was carried out using an anti-polyhistidine antibody (Thermo Fisher Scientific) diluted 1:5000 in PBS, and a secondary goat anti-mouse IgG (Fc-specific) antibody (Sigma-Aldrich, Munich, Germany) conjugated to alkaline phosphatase (diluted 1:5000 in PBS).

    Clear Native PAGE:

    Article Title: Prokaryotic nanocompartments form synthetic organelles in a eukaryote
    Article Snippet: .. Color Prestained Protein Standard, Broad Range 11–245 kDa (NEB) was used as a ladder for SDS-PAGE (marked as “M1”), while NativeMark Unstained Protein Standard (Life Technologies) was used for native PAGE (marked as “M2”). .. Gel images were captured on a ChemiDoc MP Imaging System (Bio-Rad), using the accompanying Image Lab software to approximate band intensities for densitometry measurements.

    SDS Page:

    Article Title: Generation of an artificial human B cell line test system using Transpo-mAbTM technology to evaluate the therapeutic efficacy of novel antigen-specific fusion proteins
    Article Snippet: .. Purified proteins were separated by SDS-PAGE and visualized by Coomassie Brilliant Blue staining against Color Prestained Protein Standards, Broad Range (New England Biolabs, Ipswich, Massachusetts). .. Western blot analysis was carried out using an anti-polyhistidine antibody (Thermo Fisher Scientific) diluted 1:5000 in PBS, and a secondary goat anti-mouse IgG (Fc-specific) antibody (Sigma-Aldrich, Munich, Germany) conjugated to alkaline phosphatase (diluted 1:5000 in PBS).

    Article Title: Prokaryotic nanocompartments form synthetic organelles in a eukaryote
    Article Snippet: .. Color Prestained Protein Standard, Broad Range 11–245 kDa (NEB) was used as a ladder for SDS-PAGE (marked as “M1”), while NativeMark Unstained Protein Standard (Life Technologies) was used for native PAGE (marked as “M2”). .. Gel images were captured on a ChemiDoc MP Imaging System (Bio-Rad), using the accompanying Image Lab software to approximate band intensities for densitometry measurements.

    Article Title: Structural and immunological characterization of E. coli derived recombinant CRM197 protein used as carrier in conjugate vaccines
    Article Snippet: .. SDS/PAGE molecular weight markers (New England Biolabs, Cat# P7712S) were used for molecular weight calibration. .. After Coomassie Brilliant Blue staining, the image was captured using transilluminator (Bio-Rad ChemiDoc system).

    Molecular Weight:

    Article Title: Heterologous expression of a Streptomyces cyaneus laccase for biomass modification applications
    Article Snippet: .. The protein bands were compared with P7712S molecular weight markers (New England Biolabs, Ipswich, USA). ..

    Article Title: Structural and immunological characterization of E. coli derived recombinant CRM197 protein used as carrier in conjugate vaccines
    Article Snippet: .. SDS/PAGE molecular weight markers (New England Biolabs, Cat# P7712S) were used for molecular weight calibration. .. After Coomassie Brilliant Blue staining, the image was captured using transilluminator (Bio-Rad ChemiDoc system).

    Purification:

    Article Title: Generation of an artificial human B cell line test system using Transpo-mAbTM technology to evaluate the therapeutic efficacy of novel antigen-specific fusion proteins
    Article Snippet: .. Purified proteins were separated by SDS-PAGE and visualized by Coomassie Brilliant Blue staining against Color Prestained Protein Standards, Broad Range (New England Biolabs, Ipswich, Massachusetts). .. Western blot analysis was carried out using an anti-polyhistidine antibody (Thermo Fisher Scientific) diluted 1:5000 in PBS, and a secondary goat anti-mouse IgG (Fc-specific) antibody (Sigma-Aldrich, Munich, Germany) conjugated to alkaline phosphatase (diluted 1:5000 in PBS).

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  • 93
    New England Biolabs color prestained protein standard
    Color Prestained Protein Standard, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/color prestained protein standard/product/New England Biolabs
    Average 93 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    color prestained protein standard - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

    91
    New England Biolabs polyacrylamide gel electrophoresis sds page
    Potential glycosylation sites in the HCoV-19 spike protein and hACE2. (a) 15% <t>SDS-PAGE</t> analysis of the intact and deglycosylated form of the HCoV-19 spike protein and hACE2. Molecular weight markers are shown on the left. (b) Schematic representation of the functional subunits and domains of the HCoV-19 spike protein (upper panel) and hACE2 (lower panel). (CD: connector domain; CH: central helix; CT: cytoplasmic tail; FP: fusion peptide; TM: transmembrane domain; UH: upstream helix; HR1/2: heptad repeat 1/2.) Blue indicates the domain or amino acids possibly responsible for interaction between the S protein and hACE2. Potential glycosylation sites within each domain are indicated. Red marks represent identified glycosylated sites in this study. (c–d) Mass spectra of deglycosylated peptide containing (c) N331 and N343 in the HCoV-19 spike protein and (d) N90 in hACE2.
    Polyacrylamide Gel Electrophoresis Sds Page, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 91/100, based on 0 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyacrylamide gel electrophoresis sds page/product/New England Biolabs
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    polyacrylamide gel electrophoresis sds page - by Bioz Stars, 2020-09
    91/100 stars
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    99
    New England Biolabs color prestained protein standard broad range 10 250 kda 150 gel lanes
    Potential glycosylation sites in the HCoV-19 spike protein and hACE2. (a) 15% <t>SDS-PAGE</t> analysis of the intact and deglycosylated form of the HCoV-19 spike protein and hACE2. Molecular weight markers are shown on the left. (b) Schematic representation of the functional subunits and domains of the HCoV-19 spike protein (upper panel) and hACE2 (lower panel). (CD: connector domain; CH: central helix; CT: cytoplasmic tail; FP: fusion peptide; TM: transmembrane domain; UH: upstream helix; HR1/2: heptad repeat 1/2.) Blue indicates the domain or amino acids possibly responsible for interaction between the S protein and hACE2. Potential glycosylation sites within each domain are indicated. Red marks represent identified glycosylated sites in this study. (c–d) Mass spectra of deglycosylated peptide containing (c) N331 and N343 in the HCoV-19 spike protein and (d) N90 in hACE2.
    Color Prestained Protein Standard Broad Range 10 250 Kda 150 Gel Lanes, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/color prestained protein standard broad range 10 250 kda 150 gel lanes/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    color prestained protein standard broad range 10 250 kda 150 gel lanes - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    Image Search Results


    Potential glycosylation sites in the HCoV-19 spike protein and hACE2. (a) 15% SDS-PAGE analysis of the intact and deglycosylated form of the HCoV-19 spike protein and hACE2. Molecular weight markers are shown on the left. (b) Schematic representation of the functional subunits and domains of the HCoV-19 spike protein (upper panel) and hACE2 (lower panel). (CD: connector domain; CH: central helix; CT: cytoplasmic tail; FP: fusion peptide; TM: transmembrane domain; UH: upstream helix; HR1/2: heptad repeat 1/2.) Blue indicates the domain or amino acids possibly responsible for interaction between the S protein and hACE2. Potential glycosylation sites within each domain are indicated. Red marks represent identified glycosylated sites in this study. (c–d) Mass spectra of deglycosylated peptide containing (c) N331 and N343 in the HCoV-19 spike protein and (d) N90 in hACE2.

    Journal: Engineering (Beijing, China)

    Article Title: Mass Spectrometry Analysis of Newly Emerging Coronavirus HCoV-19 Spike Protein and Human ACE2 Reveals Camouflaging Glycans and Unique Post-Translational Modifications

    doi: 10.1016/j.eng.2020.07.014

    Figure Lengend Snippet: Potential glycosylation sites in the HCoV-19 spike protein and hACE2. (a) 15% SDS-PAGE analysis of the intact and deglycosylated form of the HCoV-19 spike protein and hACE2. Molecular weight markers are shown on the left. (b) Schematic representation of the functional subunits and domains of the HCoV-19 spike protein (upper panel) and hACE2 (lower panel). (CD: connector domain; CH: central helix; CT: cytoplasmic tail; FP: fusion peptide; TM: transmembrane domain; UH: upstream helix; HR1/2: heptad repeat 1/2.) Blue indicates the domain or amino acids possibly responsible for interaction between the S protein and hACE2. Potential glycosylation sites within each domain are indicated. Red marks represent identified glycosylated sites in this study. (c–d) Mass spectra of deglycosylated peptide containing (c) N331 and N343 in the HCoV-19 spike protein and (d) N90 in hACE2.

    Article Snippet: Both the glycosylated and deglycosylated forms of the HCoV-19 spike and the hACE2 protein were then analyzed by 15% polyacrylamide gel electrophoresis (SDS-PAGE), followed by Coomassie blue staining.

    Techniques: SDS Page, Molecular Weight, Functional Assay