a1 3 4 fucosidase  (New England Biolabs)


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    Name:
    a1 3 4 Fucosidase
    Description:
    a1 3 4 Fucosidase 1 000 units
    Catalog Number:
    p0769l
    Price:
    510
    Size:
    1 000 units
    Category:
    Glycosidases
    Buy from Supplier


    Structured Review

    New England Biolabs a1 3 4 fucosidase
    a1 3 4 Fucosidase
    a1 3 4 Fucosidase 1 000 units
    https://www.bioz.com/result/a1 3 4 fucosidase/product/New England Biolabs
    Average 94 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    a1 3 4 fucosidase - by Bioz Stars, 2020-07
    94/100 stars

    Related Products / Commonly Used Together

    1xpbs
    pbs
    pngase
    osge
    neuramidase
    fucosidase treatment
    m-cscs

    Images

    1) Product Images from "The fucose salvage pathway inhibits invadopodia formation and extracellular matrix degradation in melanoma cells"

    Article Title: The fucose salvage pathway inhibits invadopodia formation and extracellular matrix degradation in melanoma cells

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0199128

    The fucose salvage pathway inhibits invadopodia through α1–2 fucosylation. A, schematic illustration showing the modification of glycans by α-1,2, α-1,3 and α-1,4 branched fucosylations. B, the effects of L-fucose and fucosidase treatment on cell surface α1–2 fucosylation, as determined by UEA-1 staining and flow cytometry. C, quantification of the effects of α-1,2 fucosidase treatment on fucose-mediated inhibition of invadopodium formation in WM793 cells. D, representative invadopodium assay images showing the effect of α-1,2 fucosidase treatment on FUK-mediated inhibition of invadopodium formation in WM793 cells. E and F, quantitation of the effect of α-1,2 fucosidase treatment on the invadopodium number per cell ( E ) and the proportion of invadopodia positive cells ( F ) in control or FUK OE WM793. G and H, quantitation of the effect of α-1,3/4 fucosidase treatment on the average invadopodium number per cell ( G ) and proportion of invadopodia positive cells ( H ) in control or fucose-treated WM793. I and J, quantitation of the effect of α-1,3/4 fucosidase treatment on the average invadopodium number per cell ( I ) and proportion of invadopodia positive cells ( J ) in control or FUK-overexpressed WM793. ** and *** indicates p
    Figure Legend Snippet: The fucose salvage pathway inhibits invadopodia through α1–2 fucosylation. A, schematic illustration showing the modification of glycans by α-1,2, α-1,3 and α-1,4 branched fucosylations. B, the effects of L-fucose and fucosidase treatment on cell surface α1–2 fucosylation, as determined by UEA-1 staining and flow cytometry. C, quantification of the effects of α-1,2 fucosidase treatment on fucose-mediated inhibition of invadopodium formation in WM793 cells. D, representative invadopodium assay images showing the effect of α-1,2 fucosidase treatment on FUK-mediated inhibition of invadopodium formation in WM793 cells. E and F, quantitation of the effect of α-1,2 fucosidase treatment on the invadopodium number per cell ( E ) and the proportion of invadopodia positive cells ( F ) in control or FUK OE WM793. G and H, quantitation of the effect of α-1,3/4 fucosidase treatment on the average invadopodium number per cell ( G ) and proportion of invadopodia positive cells ( H ) in control or fucose-treated WM793. I and J, quantitation of the effect of α-1,3/4 fucosidase treatment on the average invadopodium number per cell ( I ) and proportion of invadopodia positive cells ( J ) in control or FUK-overexpressed WM793. ** and *** indicates p

    Techniques Used: Modification, Staining, Flow Cytometry, Cytometry, Inhibition, Quantitation Assay

    2) Product Images from "The fucose salvage pathway inhibits invadopodia formation and extracellular matrix degradation in melanoma cells"

    Article Title: The fucose salvage pathway inhibits invadopodia formation and extracellular matrix degradation in melanoma cells

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0199128

    The fucose salvage pathway inhibits invadopodia through α1–2 fucosylation. A, schematic illustration showing the modification of glycans by α-1,2, α-1,3 and α-1,4 branched fucosylations. B, the effects of L-fucose and fucosidase treatment on cell surface α1–2 fucosylation, as determined by UEA-1 staining and flow cytometry. C, quantification of the effects of α-1,2 fucosidase treatment on fucose-mediated inhibition of invadopodium formation in WM793 cells. D, representative invadopodium assay images showing the effect of α-1,2 fucosidase treatment on FUK-mediated inhibition of invadopodium formation in WM793 cells. E and F, quantitation of the effect of α-1,2 fucosidase treatment on the invadopodium number per cell ( E ) and the proportion of invadopodia positive cells ( F ) in control or FUK OE WM793. G and H, quantitation of the effect of α-1,3/4 fucosidase treatment on the average invadopodium number per cell ( G ) and proportion of invadopodia positive cells ( H ) in control or fucose-treated WM793. I and J, quantitation of the effect of α-1,3/4 fucosidase treatment on the average invadopodium number per cell ( I ) and proportion of invadopodia positive cells ( J ) in control or FUK-overexpressed WM793. ** and *** indicates p
    Figure Legend Snippet: The fucose salvage pathway inhibits invadopodia through α1–2 fucosylation. A, schematic illustration showing the modification of glycans by α-1,2, α-1,3 and α-1,4 branched fucosylations. B, the effects of L-fucose and fucosidase treatment on cell surface α1–2 fucosylation, as determined by UEA-1 staining and flow cytometry. C, quantification of the effects of α-1,2 fucosidase treatment on fucose-mediated inhibition of invadopodium formation in WM793 cells. D, representative invadopodium assay images showing the effect of α-1,2 fucosidase treatment on FUK-mediated inhibition of invadopodium formation in WM793 cells. E and F, quantitation of the effect of α-1,2 fucosidase treatment on the invadopodium number per cell ( E ) and the proportion of invadopodia positive cells ( F ) in control or FUK OE WM793. G and H, quantitation of the effect of α-1,3/4 fucosidase treatment on the average invadopodium number per cell ( G ) and proportion of invadopodia positive cells ( H ) in control or fucose-treated WM793. I and J, quantitation of the effect of α-1,3/4 fucosidase treatment on the average invadopodium number per cell ( I ) and proportion of invadopodia positive cells ( J ) in control or FUK-overexpressed WM793. ** and *** indicates p

    Techniques Used: Modification, Staining, Flow Cytometry, Cytometry, Inhibition, Quantitation Assay

    Related Articles

    Staining:

    Article Title: Sialyl Lewisx-P-selectin cascade mediates tumor–mesothelial adhesion in ascitic fluid shear flow
    Article Snippet: .. In some experiments, M-CSCs were incubated with or without neuramidase (100 mU, N2876, Sigma-Aldrich), α1,3/4-fucosidase (150 mU, P0769S, New England BioLabs), OSGE (100 μg mL−1 , CLE100, Cedarlane) or PNGase (10 U mL−1 , P0704S, New England BioLabs) according to the manufacturer’s instructions for 1 h at 37 °C prior staining. .. To inhibit sulfation, M-CSCs were incubated with sodium chlorate (25 mM , 403016, Sigma-Aldrich) in serum-free medium for 48 h. To functionally block sLex , sLea or IGF-1R on M-CSCs, M-CSCs were incubated with CSLEX-1 (10 μg mL−1 , 551344, BD Biosciences), anti-CA19–9(10 μg mL−1 , ab15146, Abcam), anti-IGF-1R (2 μg mL−1 , 24–60, Invitrogen) or isotype antibodies for 1 h at 37 °C, respectively.

    Incubation:

    Article Title: The fucose salvage pathway inhibits invadopodia formation and extracellular matrix degradation in melanoma cells
    Article Snippet: .. After cell dissociation using enzyme free solution, cells were washed three times in 1xPBS, then incubated in PBS with 0.08U/mL fucosidase (New England Biolabs, α-1,2:P0724S, α-1,3/4:P0769S) for 30 minutes at 37°C. ..

    Article Title: Sialyl Lewisx-P-selectin cascade mediates tumor–mesothelial adhesion in ascitic fluid shear flow
    Article Snippet: .. In some experiments, M-CSCs were incubated with or without neuramidase (100 mU, N2876, Sigma-Aldrich), α1,3/4-fucosidase (150 mU, P0769S, New England BioLabs), OSGE (100 μg mL−1 , CLE100, Cedarlane) or PNGase (10 U mL−1 , P0704S, New England BioLabs) according to the manufacturer’s instructions for 1 h at 37 °C prior staining. .. To inhibit sulfation, M-CSCs were incubated with sodium chlorate (25 mM , 403016, Sigma-Aldrich) in serum-free medium for 48 h. To functionally block sLex , sLea or IGF-1R on M-CSCs, M-CSCs were incubated with CSLEX-1 (10 μg mL−1 , 551344, BD Biosciences), anti-CA19–9(10 μg mL−1 , ab15146, Abcam), anti-IGF-1R (2 μg mL−1 , 24–60, Invitrogen) or isotype antibodies for 1 h at 37 °C, respectively.

    Article Title: The fucose salvage pathway inhibits invadopodia formation and extracellular matrix degradation in melanoma cells
    Article Snippet: .. Fucosidase treatment After cell dissociation using enzyme free solution, cells were washed three times in 1xPBS, then incubated in PBS with 0.08U/mL fucosidase (New England Biolabs, α-1,2:P0724S, α-1,3/4:P0769S) for 30 minutes at 37°C. ..

    other:

    Article Title: A cell-free biosynthesis platform for modular construction of protein glycosylation pathways
    Article Snippet: The exoglycosidases and associated product numbers used in this study are: β1-4 Galactosidase S (P0745S); α1-3,6 Galactosidase (P0731S); α1-3,4 Fucosidase (P0769S); and α1-2 Fucosidase (P0724S); α1-3,4,6 Galactosidase (P0747S); β-N -Acetylglucosaminidase S (P0744S); α2-3 Neuraminidase S (P0743S); and α2-3,6,8 Neuraminidase (P0720S).

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  • 94
    New England Biolabs a1 3 4 fucosidase
    The fucose salvage pathway inhibits invadopodia through α1–2 fucosylation. A, schematic illustration showing the modification of glycans by α-1,2, α-1,3 and α-1,4 branched fucosylations. B, the effects of L-fucose and <t>fucosidase</t> treatment on cell surface α1–2 fucosylation, as determined by UEA-1 staining and flow cytometry. C, quantification of the effects of α-1,2 fucosidase treatment on fucose-mediated inhibition of invadopodium formation in WM793 cells. D, representative invadopodium assay images showing the effect of α-1,2 fucosidase treatment on FUK-mediated inhibition of invadopodium formation in WM793 cells. E and F, quantitation of the effect of α-1,2 fucosidase treatment on the invadopodium number per cell ( E ) and the proportion of invadopodia positive cells ( F ) in control or FUK OE WM793. G and H, quantitation of the effect of <t>α-1,3/4</t> fucosidase treatment on the average invadopodium number per cell ( G ) and proportion of invadopodia positive cells ( H ) in control or fucose-treated WM793. I and J, quantitation of the effect of α-1,3/4 fucosidase treatment on the average invadopodium number per cell ( I ) and proportion of invadopodia positive cells ( J ) in control or FUK-overexpressed WM793. ** and *** indicates p
    A1 3 4 Fucosidase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/a1 3 4 fucosidase/product/New England Biolabs
    Average 94 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    a1 3 4 fucosidase - by Bioz Stars, 2020-07
    94/100 stars
      Buy from Supplier

    Image Search Results


    The fucose salvage pathway inhibits invadopodia through α1–2 fucosylation. A, schematic illustration showing the modification of glycans by α-1,2, α-1,3 and α-1,4 branched fucosylations. B, the effects of L-fucose and fucosidase treatment on cell surface α1–2 fucosylation, as determined by UEA-1 staining and flow cytometry. C, quantification of the effects of α-1,2 fucosidase treatment on fucose-mediated inhibition of invadopodium formation in WM793 cells. D, representative invadopodium assay images showing the effect of α-1,2 fucosidase treatment on FUK-mediated inhibition of invadopodium formation in WM793 cells. E and F, quantitation of the effect of α-1,2 fucosidase treatment on the invadopodium number per cell ( E ) and the proportion of invadopodia positive cells ( F ) in control or FUK OE WM793. G and H, quantitation of the effect of α-1,3/4 fucosidase treatment on the average invadopodium number per cell ( G ) and proportion of invadopodia positive cells ( H ) in control or fucose-treated WM793. I and J, quantitation of the effect of α-1,3/4 fucosidase treatment on the average invadopodium number per cell ( I ) and proportion of invadopodia positive cells ( J ) in control or FUK-overexpressed WM793. ** and *** indicates p

    Journal: PLoS ONE

    Article Title: The fucose salvage pathway inhibits invadopodia formation and extracellular matrix degradation in melanoma cells

    doi: 10.1371/journal.pone.0199128

    Figure Lengend Snippet: The fucose salvage pathway inhibits invadopodia through α1–2 fucosylation. A, schematic illustration showing the modification of glycans by α-1,2, α-1,3 and α-1,4 branched fucosylations. B, the effects of L-fucose and fucosidase treatment on cell surface α1–2 fucosylation, as determined by UEA-1 staining and flow cytometry. C, quantification of the effects of α-1,2 fucosidase treatment on fucose-mediated inhibition of invadopodium formation in WM793 cells. D, representative invadopodium assay images showing the effect of α-1,2 fucosidase treatment on FUK-mediated inhibition of invadopodium formation in WM793 cells. E and F, quantitation of the effect of α-1,2 fucosidase treatment on the invadopodium number per cell ( E ) and the proportion of invadopodia positive cells ( F ) in control or FUK OE WM793. G and H, quantitation of the effect of α-1,3/4 fucosidase treatment on the average invadopodium number per cell ( G ) and proportion of invadopodia positive cells ( H ) in control or fucose-treated WM793. I and J, quantitation of the effect of α-1,3/4 fucosidase treatment on the average invadopodium number per cell ( I ) and proportion of invadopodia positive cells ( J ) in control or FUK-overexpressed WM793. ** and *** indicates p

    Article Snippet: Fucosidase treatment After cell dissociation using enzyme free solution, cells were washed three times in 1xPBS, then incubated in PBS with 0.08U/mL fucosidase (New England Biolabs, α-1,2:P0724S, α-1,3/4:P0769S) for 30 minutes at 37°C.

    Techniques: Modification, Staining, Flow Cytometry, Cytometry, Inhibition, Quantitation Assay

    The fucose salvage pathway inhibits invadopodia through α1–2 fucosylation. A, schematic illustration showing the modification of glycans by α-1,2, α-1,3 and α-1,4 branched fucosylations. B, the effects of L-fucose and fucosidase treatment on cell surface α1–2 fucosylation, as determined by UEA-1 staining and flow cytometry. C, quantification of the effects of α-1,2 fucosidase treatment on fucose-mediated inhibition of invadopodium formation in WM793 cells. D, representative invadopodium assay images showing the effect of α-1,2 fucosidase treatment on FUK-mediated inhibition of invadopodium formation in WM793 cells. E and F, quantitation of the effect of α-1,2 fucosidase treatment on the invadopodium number per cell ( E ) and the proportion of invadopodia positive cells ( F ) in control or FUK OE WM793. G and H, quantitation of the effect of α-1,3/4 fucosidase treatment on the average invadopodium number per cell ( G ) and proportion of invadopodia positive cells ( H ) in control or fucose-treated WM793. I and J, quantitation of the effect of α-1,3/4 fucosidase treatment on the average invadopodium number per cell ( I ) and proportion of invadopodia positive cells ( J ) in control or FUK-overexpressed WM793. ** and *** indicates p

    Journal: PLoS ONE

    Article Title: The fucose salvage pathway inhibits invadopodia formation and extracellular matrix degradation in melanoma cells

    doi: 10.1371/journal.pone.0199128

    Figure Lengend Snippet: The fucose salvage pathway inhibits invadopodia through α1–2 fucosylation. A, schematic illustration showing the modification of glycans by α-1,2, α-1,3 and α-1,4 branched fucosylations. B, the effects of L-fucose and fucosidase treatment on cell surface α1–2 fucosylation, as determined by UEA-1 staining and flow cytometry. C, quantification of the effects of α-1,2 fucosidase treatment on fucose-mediated inhibition of invadopodium formation in WM793 cells. D, representative invadopodium assay images showing the effect of α-1,2 fucosidase treatment on FUK-mediated inhibition of invadopodium formation in WM793 cells. E and F, quantitation of the effect of α-1,2 fucosidase treatment on the invadopodium number per cell ( E ) and the proportion of invadopodia positive cells ( F ) in control or FUK OE WM793. G and H, quantitation of the effect of α-1,3/4 fucosidase treatment on the average invadopodium number per cell ( G ) and proportion of invadopodia positive cells ( H ) in control or fucose-treated WM793. I and J, quantitation of the effect of α-1,3/4 fucosidase treatment on the average invadopodium number per cell ( I ) and proportion of invadopodia positive cells ( J ) in control or FUK-overexpressed WM793. ** and *** indicates p

    Article Snippet: After cell dissociation using enzyme free solution, cells were washed three times in 1xPBS, then incubated in PBS with 0.08U/mL fucosidase (New England Biolabs, α-1,2:P0724S, α-1,3/4:P0769S) for 30 minutes at 37°C.

    Techniques: Modification, Staining, Flow Cytometry, Cytometry, Inhibition, Quantitation Assay