mannosidase  (New England Biolabs)


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  • 94
    Name:
    a1 2 3 6 Mannosidase
    Description:

    Catalog Number:
    P0768
    Price:
    136
    Category:
    Glycosidases
    Applications:
    Proteomics & Glycomics
    Size:
    80 units
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    Structured Review

    New England Biolabs mannosidase
    a1 2 3 6 Mannosidase

    https://www.bioz.com/result/mannosidase/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mannosidase - by Bioz Stars, 2021-09
    94/100 stars

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    Related Articles

    other:

    Article Title: A Peptide–Lectin Fusion Strategy for Developing a Glycan Probe for Use in Various Assay Formats
    Article Snippet: EndoH and a1-2,3,6 Mannosidase obtained from New England Biolabs were used for the gp120 samples in the right and left lane, respectively, using the manufactures recommended reaction conditions.

    Article Title: Yeast surface display of full-length human microtubule-associated protein tau.
    Article Snippet: 2.3 | Deglycosylation of yeast-displayed tau Deglycosylation of yeast surface displayed tau was performed using PNGase (New England Biolabs (NEB), Cat. No. P0704S) and α1-2,3,6 Mannosidase (NEB, Cat. No. P0768S).

    Article Title: Comparison of N-linked glycosylation on hemagglutinins derived from chicken embryos and MDCK cells: a case of the production of a trivalent seasonal influenza vaccine
    Article Snippet: For enzymatic cleavage of glycans, PA-labeled glycans were digested with endoglycosidase H (Endo Hf) (New England Biolab, #P0703S, US) or α1-2,3,6 mannosidase (New England Biolab, #P0768S, US) at 37 °C overnight and inactivated at 95 °C for 10 min.

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  • 94
    New England Biolabs α1 2
    Quantification of different glycans on HAs derived from embryos and MDCK cells ( a – c ), the content of d glycans group, e high-mannose glycans, and f sulfated glycans, and the shift of the distribution of glycans post-digestion with either g <t>α1-2,3,6</t> mannosidase or h Endo Hf. The results in d , e , and f represent the average of three independent measurements. In d , error bars represent standard deviation (%). Paucimannose: N-glycans with two HexNAc and less than five Hex. High-mannose: N-glycans with two HexNAc and more than four Hex. Hybrid: N-glycans with three HexNAc. Complex: N-glycans with more than three HexNAc. In g and h , high-mannose structures were indicated by star signs, residuals of high-mannose structures were indicated by triangle signs, and residuals of hybrid structures were indicated by pound signs. CE, chicken embryo-derived HAs; MDCK, MDCK-derived HAs; GlcNAc, N-acetylglucosamine; GalNAc, N-acetylgalactosamine; Sulf-Gal, sulfurated galactose; PA, procainamide; NC, negative control, glycans without enzyme digestion; α-M, α1-2,3,6 mannosidase, glycans with α1-2,3,6 mannosidase digestion; EH, Endo Hf, glycans with Endo Hf digestion. (Fig. 3a–c: The numbers above the peaks represent the corresponding peaks numbers in the chromatogram. The pentagram labeled refers to the glycanform that it is only detected in chicken embryos-derived HA. The triangle labeled refers to the glycanform that it is only detected in MDCK cells-derived HA.)
    α1 2, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/α1 2/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    α1 2 - by Bioz Stars, 2021-09
    94/100 stars
      Buy from Supplier

    94
    New England Biolabs alpha1 2 3 6 mannosidase
    Quantification of different glycans on HAs derived from embryos and MDCK cells ( a – c ), the content of d glycans group, e high-mannose glycans, and f sulfated glycans, and the shift of the distribution of glycans post-digestion with either g <t>α1-2,3,6</t> mannosidase or h Endo Hf. The results in d , e , and f represent the average of three independent measurements. In d , error bars represent standard deviation (%). Paucimannose: N-glycans with two HexNAc and less than five Hex. High-mannose: N-glycans with two HexNAc and more than four Hex. Hybrid: N-glycans with three HexNAc. Complex: N-glycans with more than three HexNAc. In g and h , high-mannose structures were indicated by star signs, residuals of high-mannose structures were indicated by triangle signs, and residuals of hybrid structures were indicated by pound signs. CE, chicken embryo-derived HAs; MDCK, MDCK-derived HAs; GlcNAc, N-acetylglucosamine; GalNAc, N-acetylgalactosamine; Sulf-Gal, sulfurated galactose; PA, procainamide; NC, negative control, glycans without enzyme digestion; α-M, α1-2,3,6 mannosidase, glycans with α1-2,3,6 mannosidase digestion; EH, Endo Hf, glycans with Endo Hf digestion. (Fig. 3a–c: The numbers above the peaks represent the corresponding peaks numbers in the chromatogram. The pentagram labeled refers to the glycanform that it is only detected in chicken embryos-derived HA. The triangle labeled refers to the glycanform that it is only detected in MDCK cells-derived HA.)
    Alpha1 2 3 6 Mannosidase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alpha1 2 3 6 mannosidase/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    alpha1 2 3 6 mannosidase - by Bioz Stars, 2021-09
    94/100 stars
      Buy from Supplier

    Image Search Results


    Quantification of different glycans on HAs derived from embryos and MDCK cells ( a – c ), the content of d glycans group, e high-mannose glycans, and f sulfated glycans, and the shift of the distribution of glycans post-digestion with either g α1-2,3,6 mannosidase or h Endo Hf. The results in d , e , and f represent the average of three independent measurements. In d , error bars represent standard deviation (%). Paucimannose: N-glycans with two HexNAc and less than five Hex. High-mannose: N-glycans with two HexNAc and more than four Hex. Hybrid: N-glycans with three HexNAc. Complex: N-glycans with more than three HexNAc. In g and h , high-mannose structures were indicated by star signs, residuals of high-mannose structures were indicated by triangle signs, and residuals of hybrid structures were indicated by pound signs. CE, chicken embryo-derived HAs; MDCK, MDCK-derived HAs; GlcNAc, N-acetylglucosamine; GalNAc, N-acetylgalactosamine; Sulf-Gal, sulfurated galactose; PA, procainamide; NC, negative control, glycans without enzyme digestion; α-M, α1-2,3,6 mannosidase, glycans with α1-2,3,6 mannosidase digestion; EH, Endo Hf, glycans with Endo Hf digestion. (Fig. 3a–c: The numbers above the peaks represent the corresponding peaks numbers in the chromatogram. The pentagram labeled refers to the glycanform that it is only detected in chicken embryos-derived HA. The triangle labeled refers to the glycanform that it is only detected in MDCK cells-derived HA.)

    Journal: Applied Microbiology and Biotechnology

    Article Title: Comparison of N-linked glycosylation on hemagglutinins derived from chicken embryos and MDCK cells: a case of the production of a trivalent seasonal influenza vaccine

    doi: 10.1007/s00253-021-11247-5

    Figure Lengend Snippet: Quantification of different glycans on HAs derived from embryos and MDCK cells ( a – c ), the content of d glycans group, e high-mannose glycans, and f sulfated glycans, and the shift of the distribution of glycans post-digestion with either g α1-2,3,6 mannosidase or h Endo Hf. The results in d , e , and f represent the average of three independent measurements. In d , error bars represent standard deviation (%). Paucimannose: N-glycans with two HexNAc and less than five Hex. High-mannose: N-glycans with two HexNAc and more than four Hex. Hybrid: N-glycans with three HexNAc. Complex: N-glycans with more than three HexNAc. In g and h , high-mannose structures were indicated by star signs, residuals of high-mannose structures were indicated by triangle signs, and residuals of hybrid structures were indicated by pound signs. CE, chicken embryo-derived HAs; MDCK, MDCK-derived HAs; GlcNAc, N-acetylglucosamine; GalNAc, N-acetylgalactosamine; Sulf-Gal, sulfurated galactose; PA, procainamide; NC, negative control, glycans without enzyme digestion; α-M, α1-2,3,6 mannosidase, glycans with α1-2,3,6 mannosidase digestion; EH, Endo Hf, glycans with Endo Hf digestion. (Fig. 3a–c: The numbers above the peaks represent the corresponding peaks numbers in the chromatogram. The pentagram labeled refers to the glycanform that it is only detected in chicken embryos-derived HA. The triangle labeled refers to the glycanform that it is only detected in MDCK cells-derived HA.)

    Article Snippet: For enzymatic cleavage of glycans, PA-labeled glycans were digested with endoglycosidase H (Endo Hf) (New England Biolab, #P0703S, US) or α1-2,3,6 mannosidase (New England Biolab, #P0768S, US) at 37 °C overnight and inactivated at 95 °C for 10 min.

    Techniques: Derivative Assay, Standard Deviation, Negative Control, Labeling