mg2  (New England Biolabs)

Journal: Nanomaterials

Article Title: Simplified Approach for Preparing Graphene Oxide TEM Grids for Stained and Vitrified Biomolecules

doi: 10.3390/nano11030643

Figure Lengend Snippet: Monolayer GO supported TEM grids for negative staining imaging: ( a ) TEM image shows that the entire grid square is covered with a fine monolayer of GO. ( b , c ) Magnified view of each grid square. Arrows point at the near-transparent GO monolayer. A folded structure has been marked to represent complete coverage of the square. ( d – f ) Dispersion of various protein samples on GO fabricated TEM grids, observed at 80 K magnification. Arrowheads indicate each protein particle–MdtB ( d ), E. coli 70S Ribosome ( e ), VCC ( f ). ( g – i ) shows the same sample on carbon coated TEM grids (as control). ( g ) MdtB ( h ) E. coli 70S Ribosome and ( i ) VCC on carbon. GO coated TEM grids ( d – f ) attracts more biological samples.

Article Snippet: Three different biological samples, E. coli 70S Ribosome (NEB P0763S), Vibrio cholerae Cytolysin and MdtB from E. coli were visualized using this GO-coated grids.

Techniques: Transmission Electron Microscopy, Negative Staining, Imaging

Journal: Nanomaterials

Article Title: Simplified Approach for Preparing Graphene Oxide TEM Grids for Stained and Vitrified Biomolecules

doi: 10.3390/nano11030643

Figure Lengend Snippet: Application of GO methanol-water grid for cryo-EM of biological specimens: ( a – c ) MsDps, Apoferritin and E. coli 70S Ribosome on conventional holey carbon grid. ( d – f ) MsDps, Apoferritin, and E. coli 70S Ribosome on GO-Met-H 2 O grid. Relatively planar and flattened GO-Met-H 2 O coated holey grid is able to adsorb more biological samples than conventional holey carbon grid. Lower panel represents the corresponding reference-free 2D class averages of each protein specimen vitrified on our modified GO grid. 0.1 mg/mL of each sample was applied on the grids.

Article Snippet: Three different biological samples, E. coli 70S Ribosome (NEB P0763S), Vibrio cholerae Cytolysin and MdtB from E. coli were visualized using this GO-coated grids.

Techniques: Modification