e coli ribosomes  (New England Biolabs)


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    • 99
    Name:
    E coli Ribosome
    Description:
    E coli Ribosome 1 mg
    Catalog Number:
    p0763s
    Price:
    182
    Size:
    1 mg
    Category:
    Transcription Translation System Components
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    Structured Review

    New England Biolabs e coli ribosomes
    E coli Ribosome
    E coli Ribosome 1 mg
    https://www.bioz.com/result/e coli ribosomes/product/New England Biolabs
    Average 99 stars, based on 28 article reviews
    Price from $9.99 to $1999.99
    e coli ribosomes - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Ethanol Precipitation:

    Article Title: Cutting in-line with iron: ribosomal function and non-oxidative RNA cleavage
    Article Snippet: .. rRNA in-line cleavage reaction rates Nuclease free water (IDT) was used in all experiments involving purified or transcribed RNA. rRNA for in-line cleavage experiments was purified as above by phenol-chloroform extraction followed by ethanol precipitation of commercial E. coli ribosomes (New England Biolabs). .. All in-line cleavage reaction solutions were prepared and incubated in the anoxic chamber.

    Incubation:

    Article Title: Communication and quorum sensing in non-living mimics of eukaryotic cells
    Article Snippet: .. Preparation of labeled ribosomes E. coli ribosomes (New England Biolabs) were incubated in labeling buffer (50 mM HEPES pH 8.2, 100 mM KCl, 10 mM magnesium acetate) with a molar excess of Alexa Fluor 488 NHS Ester (ThermoFisher Scientific) for 90 min at room temperature. .. Free dye was removed by washing with labeling buffer in centrifugal filter devices with a molecular weight cut-off of 100 kDa.

    Article Title: Communication and quorum sensing in non-living mimics of eukaryotic cells
    Article Snippet: .. Preparation of labeled ribosomes E. coli ribosomes (New England Biolabs) were incubated in labeling buffer (50 mM HEPES pH 8.2, 100 mM KCl, 10 mM magnesium acetate) with a molar excess of Alexa Fluor 488 NHS Ester (ThermoFisher Scientific) for 90 min at room temperature. .. Free dye was removed by washing with labeling buffer in centrifugal filter devices with a molecular weight cut-off of 100 kDa.

    Article Title: Communication and quorum sensing in non-living mimics of eukaryotic cells
    Article Snippet: .. E. coli ribosomes (New England Biolabs) were incubated in labeling buffer (50 mM HEPES pH 8.2, 100 mM KCl, 10 mM magnesium acetate) with a molar excess of Alexa Fluor 488 NHS Ester (ThermoFisher Scientific) for 90 min at room temperature. .. Free dye was removed by washing with labeling buffer in centrifugal filter devices with a molecular weight cut-off of 100 kDa.

    Labeling:

    Article Title: Communication and quorum sensing in non-living mimics of eukaryotic cells
    Article Snippet: .. Preparation of labeled ribosomes E. coli ribosomes (New England Biolabs) were incubated in labeling buffer (50 mM HEPES pH 8.2, 100 mM KCl, 10 mM magnesium acetate) with a molar excess of Alexa Fluor 488 NHS Ester (ThermoFisher Scientific) for 90 min at room temperature. .. Free dye was removed by washing with labeling buffer in centrifugal filter devices with a molecular weight cut-off of 100 kDa.

    Article Title: Communication and quorum sensing in non-living mimics of eukaryotic cells
    Article Snippet: .. Preparation of labeled ribosomes E. coli ribosomes (New England Biolabs) were incubated in labeling buffer (50 mM HEPES pH 8.2, 100 mM KCl, 10 mM magnesium acetate) with a molar excess of Alexa Fluor 488 NHS Ester (ThermoFisher Scientific) for 90 min at room temperature. .. Free dye was removed by washing with labeling buffer in centrifugal filter devices with a molecular weight cut-off of 100 kDa.

    Article Title: Communication and quorum sensing in non-living mimics of eukaryotic cells
    Article Snippet: .. E. coli ribosomes (New England Biolabs) were incubated in labeling buffer (50 mM HEPES pH 8.2, 100 mM KCl, 10 mM magnesium acetate) with a molar excess of Alexa Fluor 488 NHS Ester (ThermoFisher Scientific) for 90 min at room temperature. .. Free dye was removed by washing with labeling buffer in centrifugal filter devices with a molecular weight cut-off of 100 kDa.

    Purification:

    Article Title: Fast and Highly Efficient Affinity Enrichment of Azide-A-DSBSO Cross-Linked Peptides.
    Article Snippet: .. Materials Purified E. coli ribosome (E. coli B strain) was purchased from New England Biolabs (MA, USA) and diluted with dilution buffer (50 mM HEPES, 50 mM KCl, pH 7.5, 10 mM MgAc2) to a concentration of 1 mg/mL. .. Purified recombinant Cas9 from S. pyogenes fused with a Halo-tag was generated in house, as described by Deng et al.25 DSBSO was synthesized similarly as described by Burke et al.24 For enrichment similar as described by Kaake et al.15 (BARAC Method), Dibenzocyclooctyne-PEG4-biotin conjugate (#760749-5MG, SigmaAldrich) was used without further purification.

    Article Title: Cutting in-line with iron: ribosomal function and non-oxidative RNA cleavage
    Article Snippet: .. rRNA in-line cleavage reaction rates Nuclease free water (IDT) was used in all experiments involving purified or transcribed RNA. rRNA for in-line cleavage experiments was purified as above by phenol-chloroform extraction followed by ethanol precipitation of commercial E. coli ribosomes (New England Biolabs). .. All in-line cleavage reaction solutions were prepared and incubated in the anoxic chamber.

    Concentration Assay:

    Article Title: Fast and Highly Efficient Affinity Enrichment of Azide-A-DSBSO Cross-Linked Peptides.
    Article Snippet: .. Materials Purified E. coli ribosome (E. coli B strain) was purchased from New England Biolabs (MA, USA) and diluted with dilution buffer (50 mM HEPES, 50 mM KCl, pH 7.5, 10 mM MgAc2) to a concentration of 1 mg/mL. .. Purified recombinant Cas9 from S. pyogenes fused with a Halo-tag was generated in house, as described by Deng et al.25 DSBSO was synthesized similarly as described by Burke et al.24 For enrichment similar as described by Kaake et al.15 (BARAC Method), Dibenzocyclooctyne-PEG4-biotin conjugate (#760749-5MG, SigmaAldrich) was used without further purification.

    Generated:

    Article Title: In vitro ribosome synthesis and evolution through ribosome display
    Article Snippet: .. A standard curve was generated from a dilution series of 70 S E. coli ribosomes (NEB) to ensure linearity of the assay (Supplementary Fig. ). ..

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    • e coli ribosomes  (New England Biolabs)
    99
    New England Biolabs e coli ribosomes
    E Coli Ribosomes, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli ribosomes/product/New England Biolabs
    Average 99 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    e coli ribosomes - by Bioz Stars, 2020-09
    99/100 stars
    		  Buy from Supplier

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