p nitrophenylphosphate  (New England Biolabs)


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    Name:
    p Nitrophenylphosphate PNPP
    Description:
    p Nitrophenylphosphate PNPP 5 ml
    Catalog Number:
    p0757l
    Price:
    131
    Size:
    5 ml
    Category:
    Biochemical Reagents
    Buy from Supplier


    Structured Review

    New England Biolabs p nitrophenylphosphate
    p Nitrophenylphosphate PNPP
    p Nitrophenylphosphate PNPP 5 ml
    https://www.bioz.com/result/p nitrophenylphosphate/product/New England Biolabs
    Average 93 stars, based on 31 article reviews
    Price from $9.99 to $1999.99
    p nitrophenylphosphate - by Bioz Stars, 2021-01
    93/100 stars

    Images

    1) Product Images from "The Novel Pro-Osteogenic Activity of NUCB21–83"

    Article Title: The Novel Pro-Osteogenic Activity of NUCB21–83

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0061619

    The effect of NUCB2 1–83 on osteoclast differentiation quantified by measuring TRAP activity. RAW 264.7 cells were cultured in the presence of RANKL (50 ng/mL) for 5 days with or without NUCB2 1–83. The cells were fixed and incubated in 10 mM citrate buffer (pH 4.6) containing 10 mM sodium tratrate and 5 mM p-nitrophenylphosphate for 1 h followed by transferring into new well plates containing an equal volume of 0.1 N NaOH. TRAP activity was measured at λ = 405 nm and expressed as percent of that of untreated control. Data represented the mean±SEM(*P
    Figure Legend Snippet: The effect of NUCB2 1–83 on osteoclast differentiation quantified by measuring TRAP activity. RAW 264.7 cells were cultured in the presence of RANKL (50 ng/mL) for 5 days with or without NUCB2 1–83. The cells were fixed and incubated in 10 mM citrate buffer (pH 4.6) containing 10 mM sodium tratrate and 5 mM p-nitrophenylphosphate for 1 h followed by transferring into new well plates containing an equal volume of 0.1 N NaOH. TRAP activity was measured at λ = 405 nm and expressed as percent of that of untreated control. Data represented the mean±SEM(*P

    Techniques Used: Activity Assay, Cell Culture, Incubation, Transferring

    2) Product Images from "The Effect of pstS and phoB on Quorum Sensing and Swarming Motility in Pseudomonas aeruginosa"

    Article Title: The Effect of pstS and phoB on Quorum Sensing and Swarming Motility in Pseudomonas aeruginosa

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0074444

    The effect of pstS and phoB deletion on phosphate starvation in P.aeruginosa . PAO1, Δ pstS , Δ phoB and Δ pstS Δ phoB were grown for 24 hours at 37°C on swarming plates containing M9 or phosphate-depleted M9. After 24 hours, bacteria were scraped off the plates and Alkaline Phosphatase activity was measured using p-Nitrophenyl Phosphate as described in Materials and Methods. Results were normalized to each samples’ total protein concentration using Bradford assay. Results shown represent mean+standard deviation of six different experiments. Each experiment was performed in triplicate. Asterisks represent the significant rise in AP activity compared to the WT (p
    Figure Legend Snippet: The effect of pstS and phoB deletion on phosphate starvation in P.aeruginosa . PAO1, Δ pstS , Δ phoB and Δ pstS Δ phoB were grown for 24 hours at 37°C on swarming plates containing M9 or phosphate-depleted M9. After 24 hours, bacteria were scraped off the plates and Alkaline Phosphatase activity was measured using p-Nitrophenyl Phosphate as described in Materials and Methods. Results were normalized to each samples’ total protein concentration using Bradford assay. Results shown represent mean+standard deviation of six different experiments. Each experiment was performed in triplicate. Asterisks represent the significant rise in AP activity compared to the WT (p

    Techniques Used: Activity Assay, Protein Concentration, Bradford Assay, Standard Deviation

    3) Product Images from "Rcs Phosphorelay Activation in Cardiolipin-Deficient Escherichia coli Reduces Biofilm Formation"

    Article Title: Rcs Phosphorelay Activation in Cardiolipin-Deficient Escherichia coli Reduces Biofilm Formation

    Journal: Journal of Bacteriology

    doi: 10.1128/JB.00804-18

    Protein translocation is reduced in the absence of CL. (A and B) Periplasmic activity of PhoA in wild-type MG1655, Δ clsABC , and Δ secG strains; 1 unit equals 1 mol/min of hydrolyzed p -nitrophenyl phosphate (PNPP). Measurements represent the average of 3 biological replicates, and error bars indicate standard error of the mean.
    Figure Legend Snippet: Protein translocation is reduced in the absence of CL. (A and B) Periplasmic activity of PhoA in wild-type MG1655, Δ clsABC , and Δ secG strains; 1 unit equals 1 mol/min of hydrolyzed p -nitrophenyl phosphate (PNPP). Measurements represent the average of 3 biological replicates, and error bars indicate standard error of the mean.

    Techniques Used: Translocation Assay, Activity Assay

    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: The Effect of pstS and phoB on Quorum Sensing and Swarming Motility in Pseudomonas aeruginosa
    Article Snippet: .. 5 µl of 500 mM p-Nitrophenyl Phosphate (PNPP, NEB) were added to each well and the reaction was read at 405 nm in an ELISA plate reader )Synergy™ 2 Multi-Detection Microplate Reader; Biotech). ..

    Incubation:

    Article Title: MYC dephosphorylation by the PP1/PNUTS phosphatase complex regulates chromatin binding and protein stability
    Article Snippet: .. These samples were then incubated with the substrate 50 mM PNP (P0757, New England BioLabs) in the above mentioned buffer for CIP or PP1 for 5 min or 30 min respectively at 30 °C in a 50 μL reaction volume. .. The reaction was stopped with 1 mL 1 N NaOH and the amount of p- Nitrophenol formed was determined by spectrophotometer absorbance at 405 nM (molar extinction coefficient 18,000 M-1 cm-1).

    Article Title: The Novel Pro-Osteogenic Activity of NUCB21–83
    Article Snippet: .. Subsequently, the dried cells were incubated in 10 mM citrate buffer (pH 4.6) containing 10 mM sodium tratrate (Sigma-Aldrich St. Louis, MO) and 5 mM p-nitrophenylphosphate (New England BioLabs, Beverly, MA, USA ). .. After incubation for 1 h, the reaction mixtures were transferred into new well plates containing an equal volume of 0.1 N NaOH.

    Activity Assay:

    Article Title: Evolution of Bacterial Phosphoglycerate Mutases: Non-Homologous Isofunctional Enzymes Undergoing Gene Losses, Gains and Lateral Transfers
    Article Snippet: .. However, we could not detect any phosphatase activity when the protein was assayed against the general phosphatase substrate, p -nitrophenyl phosphate, using buffers and metal ions (Mg2+ , Co2+ or Zn2+ ) preferred by bacterial alkaline phosphatases . .. Our alkaline phosphatase positive control, calf intestinal phosphatase, was active under all conditions tested (data not shown).

    Imaging:

    Article Title: Redirecting SR Protein Nuclear Trafficking Through An Allosteric Platform
    Article Snippet: .. ATP, Mops, HEPES, Tris, MgCl2 , MnCl2 , NaCl, EDTA, NP40, Brij 35, glycerol, sucrose, acetic acid, lysozyme, DNAse, RNAse, Phenix imaging film, BSA, Protein G–agarose Ni-resin and liquid scintillant were obtained from Fisher Scientific. γ32 P-ATP was obtained from NEN Products. p-Nitrophenyl Phosphate and 10xPMP buffer (500 mM HEPES, 1 M NaCl, 20 mM DTT, 0.1% Brij 35) were purchased from NEB. .. Protease inhibitor cocktail was obtained from Roche and anti-PP1γ monoclonal antibody was purchased from Thermo, and anti-GFP monoclonal antibody was purchased from Cell Signaling.

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  • 93
    New England Biolabs p nitrophenylphosphate
    The effect of NUCB2 1–83 on osteoclast differentiation quantified by measuring TRAP activity. RAW 264.7 cells were cultured in the presence of RANKL (50 ng/mL) for 5 days with or without NUCB2 1–83. The cells were fixed and incubated in 10 mM citrate buffer (pH 4.6) containing 10 mM sodium tratrate and 5 mM <t>p-nitrophenylphosphate</t> for 1 h followed by transferring into new well plates containing an equal volume of 0.1 N NaOH. TRAP activity was measured at λ = 405 nm and expressed as percent of that of untreated control. Data represented the mean±SEM(*P
    P Nitrophenylphosphate, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p nitrophenylphosphate/product/New England Biolabs
    Average 93 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    p nitrophenylphosphate - by Bioz Stars, 2021-01
    93/100 stars
      Buy from Supplier

    Image Search Results


    The effect of NUCB2 1–83 on osteoclast differentiation quantified by measuring TRAP activity. RAW 264.7 cells were cultured in the presence of RANKL (50 ng/mL) for 5 days with or without NUCB2 1–83. The cells were fixed and incubated in 10 mM citrate buffer (pH 4.6) containing 10 mM sodium tratrate and 5 mM p-nitrophenylphosphate for 1 h followed by transferring into new well plates containing an equal volume of 0.1 N NaOH. TRAP activity was measured at λ = 405 nm and expressed as percent of that of untreated control. Data represented the mean±SEM(*P

    Journal: PLoS ONE

    Article Title: The Novel Pro-Osteogenic Activity of NUCB21–83

    doi: 10.1371/journal.pone.0061619

    Figure Lengend Snippet: The effect of NUCB2 1–83 on osteoclast differentiation quantified by measuring TRAP activity. RAW 264.7 cells were cultured in the presence of RANKL (50 ng/mL) for 5 days with or without NUCB2 1–83. The cells were fixed and incubated in 10 mM citrate buffer (pH 4.6) containing 10 mM sodium tratrate and 5 mM p-nitrophenylphosphate for 1 h followed by transferring into new well plates containing an equal volume of 0.1 N NaOH. TRAP activity was measured at λ = 405 nm and expressed as percent of that of untreated control. Data represented the mean±SEM(*P

    Article Snippet: Subsequently, the dried cells were incubated in 10 mM citrate buffer (pH 4.6) containing 10 mM sodium tratrate (Sigma-Aldrich St. Louis, MO) and 5 mM p-nitrophenylphosphate (New England BioLabs, Beverly, MA, USA ).

    Techniques: Activity Assay, Cell Culture, Incubation, Transferring

    The effect of pstS and phoB deletion on phosphate starvation in P.aeruginosa . PAO1, Δ pstS , Δ phoB and Δ pstS Δ phoB were grown for 24 hours at 37°C on swarming plates containing M9 or phosphate-depleted M9. After 24 hours, bacteria were scraped off the plates and Alkaline Phosphatase activity was measured using p-Nitrophenyl Phosphate as described in Materials and Methods. Results were normalized to each samples’ total protein concentration using Bradford assay. Results shown represent mean+standard deviation of six different experiments. Each experiment was performed in triplicate. Asterisks represent the significant rise in AP activity compared to the WT (p

    Journal: PLoS ONE

    Article Title: The Effect of pstS and phoB on Quorum Sensing and Swarming Motility in Pseudomonas aeruginosa

    doi: 10.1371/journal.pone.0074444

    Figure Lengend Snippet: The effect of pstS and phoB deletion on phosphate starvation in P.aeruginosa . PAO1, Δ pstS , Δ phoB and Δ pstS Δ phoB were grown for 24 hours at 37°C on swarming plates containing M9 or phosphate-depleted M9. After 24 hours, bacteria were scraped off the plates and Alkaline Phosphatase activity was measured using p-Nitrophenyl Phosphate as described in Materials and Methods. Results were normalized to each samples’ total protein concentration using Bradford assay. Results shown represent mean+standard deviation of six different experiments. Each experiment was performed in triplicate. Asterisks represent the significant rise in AP activity compared to the WT (p

    Article Snippet: 5 µl of 500 mM p-Nitrophenyl Phosphate (PNPP, NEB) were added to each well and the reaction was read at 405 nm in an ELISA plate reader )Synergy™ 2 Multi-Detection Microplate Reader; Biotech).

    Techniques: Activity Assay, Protein Concentration, Bradford Assay, Standard Deviation

    Protein translocation is reduced in the absence of CL. (A and B) Periplasmic activity of PhoA in wild-type MG1655, Δ clsABC , and Δ secG strains; 1 unit equals 1 mol/min of hydrolyzed p -nitrophenyl phosphate (PNPP). Measurements represent the average of 3 biological replicates, and error bars indicate standard error of the mean.

    Journal: Journal of Bacteriology

    Article Title: Rcs Phosphorelay Activation in Cardiolipin-Deficient Escherichia coli Reduces Biofilm Formation

    doi: 10.1128/JB.00804-18

    Figure Lengend Snippet: Protein translocation is reduced in the absence of CL. (A and B) Periplasmic activity of PhoA in wild-type MG1655, Δ clsABC , and Δ secG strains; 1 unit equals 1 mol/min of hydrolyzed p -nitrophenyl phosphate (PNPP). Measurements represent the average of 3 biological replicates, and error bars indicate standard error of the mean.

    Article Snippet: Cell suspensions were vortexed briefly, and then 100 μl of 4 mg/ml p -nitrophenyl phosphate (New England Biolabs, MA) was added and the mixture was vortexed again.

    Techniques: Translocation Assay, Activity Assay