p nitrophenylphosphate  (New England Biolabs)


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    Name:
    p Nitrophenylphosphate PNPP
    Description:
    p Nitrophenylphosphate PNPP 5 ml
    Catalog Number:
    p0757l
    Price:
    131
    Size:
    5 ml
    Category:
    Biochemical Reagents
    Buy from Supplier


    Structured Review

    New England Biolabs p nitrophenylphosphate
    p Nitrophenylphosphate PNPP
    p Nitrophenylphosphate PNPP 5 ml
    https://www.bioz.com/result/p nitrophenylphosphate/product/New England Biolabs
    Average 96 stars, based on 17 article reviews
    Price from $9.99 to $1999.99
    p nitrophenylphosphate - by Bioz Stars, 2020-08
    96/100 stars

    Images

    1) Product Images from "The Novel Pro-Osteogenic Activity of NUCB21–83"

    Article Title: The Novel Pro-Osteogenic Activity of NUCB21–83

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0061619

    The effect of NUCB2 1–83 on osteoclast differentiation quantified by measuring TRAP activity. RAW 264.7 cells were cultured in the presence of RANKL (50 ng/mL) for 5 days with or without NUCB2 1–83. The cells were fixed and incubated in 10 mM citrate buffer (pH 4.6) containing 10 mM sodium tratrate and 5 mM p-nitrophenylphosphate for 1 h followed by transferring into new well plates containing an equal volume of 0.1 N NaOH. TRAP activity was measured at λ = 405 nm and expressed as percent of that of untreated control. Data represented the mean±SEM(*P
    Figure Legend Snippet: The effect of NUCB2 1–83 on osteoclast differentiation quantified by measuring TRAP activity. RAW 264.7 cells were cultured in the presence of RANKL (50 ng/mL) for 5 days with or without NUCB2 1–83. The cells were fixed and incubated in 10 mM citrate buffer (pH 4.6) containing 10 mM sodium tratrate and 5 mM p-nitrophenylphosphate for 1 h followed by transferring into new well plates containing an equal volume of 0.1 N NaOH. TRAP activity was measured at λ = 405 nm and expressed as percent of that of untreated control. Data represented the mean±SEM(*P

    Techniques Used: Activity Assay, Cell Culture, Incubation, Transferring

    2) Product Images from "The Effect of pstS and phoB on Quorum Sensing and Swarming Motility in Pseudomonas aeruginosa"

    Article Title: The Effect of pstS and phoB on Quorum Sensing and Swarming Motility in Pseudomonas aeruginosa

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0074444

    The effect of pstS and phoB deletion on phosphate starvation in P.aeruginosa . PAO1, Δ pstS , Δ phoB and Δ pstS Δ phoB were grown for 24 hours at 37°C on swarming plates containing M9 or phosphate-depleted M9. After 24 hours, bacteria were scraped off the plates and Alkaline Phosphatase activity was measured using p-Nitrophenyl Phosphate as described in Materials and Methods. Results were normalized to each samples’ total protein concentration using Bradford assay. Results shown represent mean+standard deviation of six different experiments. Each experiment was performed in triplicate. Asterisks represent the significant rise in AP activity compared to the WT (p
    Figure Legend Snippet: The effect of pstS and phoB deletion on phosphate starvation in P.aeruginosa . PAO1, Δ pstS , Δ phoB and Δ pstS Δ phoB were grown for 24 hours at 37°C on swarming plates containing M9 or phosphate-depleted M9. After 24 hours, bacteria were scraped off the plates and Alkaline Phosphatase activity was measured using p-Nitrophenyl Phosphate as described in Materials and Methods. Results were normalized to each samples’ total protein concentration using Bradford assay. Results shown represent mean+standard deviation of six different experiments. Each experiment was performed in triplicate. Asterisks represent the significant rise in AP activity compared to the WT (p

    Techniques Used: Activity Assay, Protein Concentration, Bradford Assay, Standard Deviation

    Related Articles

    Colorimetric Assay:

    Article Title: Conditional antagonism in co-cultures of Pseudomonas aeruginosa and Candida albicans: an intersection of ethanol and phosphate signaling distilled from dual-seq transcriptomics
    Article Snippet: .. pNPP For quantification of AP activity, we used a colorimetric assay using p-Nitrophenyl phosphate (pNPP) (NEB) as a substrate. .. Briefly, 5 μl of P. aeruginosa overnight culture were inoculated onto MOPS 0.7 mM phosphate agar plates with and without 1% ethanol and incubated at 37°C for 16 hours.

    Enzyme-linked Immunosorbent Assay:

    Article Title: The Effect of pstS and phoB on Quorum Sensing and Swarming Motility in Pseudomonas aeruginosa
    Article Snippet: .. 5 µl of 500 mM p-Nitrophenyl Phosphate (PNPP, NEB) were added to each well and the reaction was read at 405 nm in an ELISA plate reader )Synergy™ 2 Multi-Detection Microplate Reader; Biotech). ..

    ALP Assay:

    Article Title: Colorimetric Immunoassay for Detection of Tumor Markers
    Article Snippet: .. The most widely used ALP substrate is p-nitrophenyl-phosphate (pNPP: Supplier: New England BioLabs; Catalog No. P0757S). ..

    Incubation:

    Article Title: MYC dephosphorylation by the PP1/PNUTS phosphatase complex regulates chromatin binding and protein stability
    Article Snippet: .. These samples were then incubated with the substrate 50 mM PNP (P0757, New England BioLabs) in the above mentioned buffer for CIP or PP1 for 5 min or 30 min respectively at 30 °C in a 50 μL reaction volume. .. The reaction was stopped with 1 mL 1 N NaOH and the amount of p- Nitrophenol formed was determined by spectrophotometer absorbance at 405 nM (molar extinction coefficient 18,000 M-1 cm-1).

    Article Title: The Novel Pro-Osteogenic Activity of NUCB21–83
    Article Snippet: .. Subsequently, the dried cells were incubated in 10 mM citrate buffer (pH 4.6) containing 10 mM sodium tratrate (Sigma-Aldrich St. Louis, MO) and 5 mM p-nitrophenylphosphate (New England BioLabs, Beverly, MA, USA ). .. After incubation for 1 h, the reaction mixtures were transferred into new well plates containing an equal volume of 0.1 N NaOH.

    Activity Assay:

    Article Title: Conditional antagonism in co-cultures of Pseudomonas aeruginosa and Candida albicans: an intersection of ethanol and phosphate signaling distilled from dual-seq transcriptomics
    Article Snippet: .. pNPP For quantification of AP activity, we used a colorimetric assay using p-Nitrophenyl phosphate (pNPP) (NEB) as a substrate. .. Briefly, 5 μl of P. aeruginosa overnight culture were inoculated onto MOPS 0.7 mM phosphate agar plates with and without 1% ethanol and incubated at 37°C for 16 hours.

    Article Title: Porphyromonas gingivalis Tyrosine Phosphatase Php1 Promotes Community Development and Pathogenicity
    Article Snippet: .. To measure phosphatase activity of recombinant Php1, p -nitrophenyl phosphate (p NPP; New England Biolabs), phospho-amino acids (phosphotyrosine [Sigma], phosphoserine [Sigma], and phosphothreonine [Sigma]), or phosphopeptides (tyrosine phosphopeptide ENDpYINASL [Promega] and serine phosphopeptide DLDVPIPGRFDRRVpSVAAE [Ser/Thr phosphatase substrate I; R & D Systems]) were used as substrates. ..

    Article Title: Combining secondary-structure and protein solvent-accessibility predictions in methionine substitution for anomalous dispersion
    Article Snippet: .. p -Nitrophenyl phosphate ( p NPP; New England Biolabs) was used at a series of concentrations to determine the phosphatase activity of At TLP18.3. .. The incubated solution consisted of 15 µg purified At TLP18.3, 50 m M sodium acetate buffer pH 4.0 and the substrate to give a total volume of 0.1 ml.

    Article Title: Mycobacterium tuberculosis SatS is a chaperone for the SecA2 protein export pathway
    Article Snippet: .. In a 96 well plate, 3 µg of CFP protein was diluted with water and added to 10X buffer (1M Tris base pH 6.8) with 20 mM Sodium tartrate to reduce background phosphatase activity, and 50 mM p-nitrophenyl phosphate (pNPP) for a total volume of 200 µL (New England Biolabs, Ipswich, MA). .. Tartrate is an inhibitor of some phosphatases, but SapM activity is unaffected by tartrate ( ).

    Recombinant:

    Article Title: Porphyromonas gingivalis Tyrosine Phosphatase Php1 Promotes Community Development and Pathogenicity
    Article Snippet: .. To measure phosphatase activity of recombinant Php1, p -nitrophenyl phosphate (p NPP; New England Biolabs), phospho-amino acids (phosphotyrosine [Sigma], phosphoserine [Sigma], and phosphothreonine [Sigma]), or phosphopeptides (tyrosine phosphopeptide ENDpYINASL [Promega] and serine phosphopeptide DLDVPIPGRFDRRVpSVAAE [Ser/Thr phosphatase substrate I; R & D Systems]) were used as substrates. ..

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  • 96
    New England Biolabs p nitrophenylphosphate
    The effect of NUCB2 1–83 on osteoclast differentiation quantified by measuring TRAP activity. RAW 264.7 cells were cultured in the presence of RANKL (50 ng/mL) for 5 days with or without NUCB2 1–83. The cells were fixed and incubated in 10 mM citrate buffer (pH 4.6) containing 10 mM sodium tratrate and 5 mM <t>p-nitrophenylphosphate</t> for 1 h followed by transferring into new well plates containing an equal volume of 0.1 N NaOH. TRAP activity was measured at λ = 405 nm and expressed as percent of that of untreated control. Data represented the mean±SEM(*P
    P Nitrophenylphosphate, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p nitrophenylphosphate/product/New England Biolabs
    Average 96 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    p nitrophenylphosphate - by Bioz Stars, 2020-08
    96/100 stars
      Buy from Supplier

    Image Search Results


    The effect of NUCB2 1–83 on osteoclast differentiation quantified by measuring TRAP activity. RAW 264.7 cells were cultured in the presence of RANKL (50 ng/mL) for 5 days with or without NUCB2 1–83. The cells were fixed and incubated in 10 mM citrate buffer (pH 4.6) containing 10 mM sodium tratrate and 5 mM p-nitrophenylphosphate for 1 h followed by transferring into new well plates containing an equal volume of 0.1 N NaOH. TRAP activity was measured at λ = 405 nm and expressed as percent of that of untreated control. Data represented the mean±SEM(*P

    Journal: PLoS ONE

    Article Title: The Novel Pro-Osteogenic Activity of NUCB21–83

    doi: 10.1371/journal.pone.0061619

    Figure Lengend Snippet: The effect of NUCB2 1–83 on osteoclast differentiation quantified by measuring TRAP activity. RAW 264.7 cells were cultured in the presence of RANKL (50 ng/mL) for 5 days with or without NUCB2 1–83. The cells were fixed and incubated in 10 mM citrate buffer (pH 4.6) containing 10 mM sodium tratrate and 5 mM p-nitrophenylphosphate for 1 h followed by transferring into new well plates containing an equal volume of 0.1 N NaOH. TRAP activity was measured at λ = 405 nm and expressed as percent of that of untreated control. Data represented the mean±SEM(*P

    Article Snippet: Subsequently, the dried cells were incubated in 10 mM citrate buffer (pH 4.6) containing 10 mM sodium tratrate (Sigma-Aldrich St. Louis, MO) and 5 mM p-nitrophenylphosphate (New England BioLabs, Beverly, MA, USA ).

    Techniques: Activity Assay, Cell Culture, Incubation, Transferring

    The effect of pstS and phoB deletion on phosphate starvation in P.aeruginosa . PAO1, Δ pstS , Δ phoB and Δ pstS Δ phoB were grown for 24 hours at 37°C on swarming plates containing M9 or phosphate-depleted M9. After 24 hours, bacteria were scraped off the plates and Alkaline Phosphatase activity was measured using p-Nitrophenyl Phosphate as described in Materials and Methods. Results were normalized to each samples’ total protein concentration using Bradford assay. Results shown represent mean+standard deviation of six different experiments. Each experiment was performed in triplicate. Asterisks represent the significant rise in AP activity compared to the WT (p

    Journal: PLoS ONE

    Article Title: The Effect of pstS and phoB on Quorum Sensing and Swarming Motility in Pseudomonas aeruginosa

    doi: 10.1371/journal.pone.0074444

    Figure Lengend Snippet: The effect of pstS and phoB deletion on phosphate starvation in P.aeruginosa . PAO1, Δ pstS , Δ phoB and Δ pstS Δ phoB were grown for 24 hours at 37°C on swarming plates containing M9 or phosphate-depleted M9. After 24 hours, bacteria were scraped off the plates and Alkaline Phosphatase activity was measured using p-Nitrophenyl Phosphate as described in Materials and Methods. Results were normalized to each samples’ total protein concentration using Bradford assay. Results shown represent mean+standard deviation of six different experiments. Each experiment was performed in triplicate. Asterisks represent the significant rise in AP activity compared to the WT (p

    Article Snippet: 5 µl of 500 mM p-Nitrophenyl Phosphate (PNPP, NEB) were added to each well and the reaction was read at 405 nm in an ELISA plate reader )Synergy™ 2 Multi-Detection Microplate Reader; Biotech).

    Techniques: Activity Assay, Protein Concentration, Bradford Assay, Standard Deviation