β galactosidase assay  (New England Biolabs)


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    Name:
    beta 1 4 Galactosidase S
    Description:
    beta 1 4 Galactosidase S 2 000 units
    Catalog Number:
    p0745l
    Price:
    528
    Size:
    2 000 units
    Category:
    Glycosidases
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    Structured Review

    New England Biolabs β galactosidase assay
    beta 1 4 Galactosidase S
    beta 1 4 Galactosidase S 2 000 units
    https://www.bioz.com/result/β galactosidase assay/product/New England Biolabs
    Average 94 stars, based on 21 article reviews
    Price from $9.99 to $1999.99
    β galactosidase assay - by Bioz Stars, 2021-02
    94/100 stars

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    Related Articles

    Hydrophilic Interaction Liquid Chromatography:

    Article Title: Measuring site-specific glycosylation similarity between influenza A virus variants with statistical certainty
    Article Snippet: .. The second aliquot was incubated overnight at 37 °C with β1-4 galactosidase (New England Biolabs #P0745) using the same reaction conditions as the neuraminidase above, and then HILIC-enriched. ..

    High Performance Liquid Chromatography:

    Article Title: Comparative analysis of involvement of UGT1 and UGT2 splice variants of UDP-galactose transporter in glycosylation of macromolecules in MDCK and CHO cell lines
    Article Snippet: .. The rest of the sample was digested with 1-3,4 β-galactosidase (New England Biolabs), filtered using an Ultrafree MC spin column, dried, re-suspended in 10 μl of 70% acetonitrile, .and separated on a GlycoSep N column using HPLC. .. Analysis of proteoglycans by Western blotting Aliquots containing 20 μg of total proteins extracted from cells grown in respective serum-free media were separated by SDS-PAGE in 8% gels and transferred onto nitrocellulose membranes (Whatman).

    In Vivo:

    Article Title: Ornithine capture by a translating ribosome controls bacterial polyamine synthesis
    Article Snippet: .. β-galactosidase assay To test for in vivo activity, a translational reporter plasmid was obtained by fusing a region containing speFL , the speFL–speF intergenic region and the first three codons of speF to lacZα. .. The insert was prepared by PCR amplification from the E. coli K12 genome using oligonucleotides 7 and 8.

    Luciferase:

    Article Title: Protein delivery into live cells by incubation with an endosomolytic agent
    Article Snippet: .. Guy Sauvageau (Montreal University, Montreal, Quebec, Canada) for pTAT-HA-HoxB4, Dr. Peter Zandstra (University of Toronto, Ontario, Canada) for the HoxB4 luciferase reporter, engineered 3T3 cells, and β-gal vectors and Dr. Ivan R. Correa (New England Biolabs, Ipswich, MA) for pSNAP-H2B and SNAP-Surface 488. .. We also thank Dr. James Sacchettinni (Texas A & M University, College Station, TX) for providing HDF and Neuro-2a cells, Dr. Eric Shoubridge (Montreal Neurological Institute and Hospital, Montreal, Quebec, Canada) for MCH58 cells and Dr. Guoyao Wu (Texas A & M University, College Station, TX) for primary intestinal porcine epithelial cells.

    Article Title: Targeted Expression of Suicide Gene by Tissue-Specific Promoter and MicroRNA Regulation for Cancer Gene Therapy
    Article Snippet: .. The cells were harvested and analysed for luciferase and β-galactosidase activities in accordance with the manufacturer’s protocols (New England Biolabs). .. Western blot analysis In order to analyse the expression of TK and EpCAM proteins, total cell extracts were prepared by lysing the cells in the lysis buffer [10 mM Tris-HCl pH 7.2, 2% SDS, 10 mM dithiothreitol, 1% protease and phosphatase inhibitors cocktail (Sigma Aldrich, MO, USA)].

    Transfection:

    Article Title: Oligomerization of the Tetraspanin CD81 via the Flexibility of Its δ-Loop
    Article Snippet: .. In brief, 105 Jurkat T-cells transfected with a plasmid encoding for CD81-GFP were plated onto poly-lysine-coated coverslips in 1 mL prewarmed Ringer solution (130 mM NaCl, 4 mM KCl, 1 mM CaCl2 , 1 mM MgCl2 , 48 mM D(+)glucose, 10 mM HEPES, pH 7.4) for control samples or prewarmed Ringer solution supplemented with 0.5 unit/mL neuraminidase (#N2876; Sigma-Aldrich, St. Louis, MO) and 4 units/mL β 1-4 galactosidase (#P0730; New England Biolabs, Ipswich, MA) for treated samples. .. Cell suspensions were plated on poly-L-lysine-coated coverslips in six-well plates and incubated for 1 h in a cell incubator.

    Incubation:

    Article Title: Measuring site-specific glycosylation similarity between influenza A virus variants with statistical certainty
    Article Snippet: .. The second aliquot was incubated overnight at 37 °C with β1-4 galactosidase (New England Biolabs #P0745) using the same reaction conditions as the neuraminidase above, and then HILIC-enriched. ..

    Activity Assay:

    Article Title: Ornithine capture by a translating ribosome controls bacterial polyamine synthesis
    Article Snippet: .. β-galactosidase assay To test for in vivo activity, a translational reporter plasmid was obtained by fusing a region containing speFL , the speFL–speF intergenic region and the first three codons of speF to lacZα. .. The insert was prepared by PCR amplification from the E. coli K12 genome using oligonucleotides 7 and 8.

    Liquid Chromatography with Mass Spectroscopy:

    Article Title: Increased sialylation of site specific O-glycoforms of hemopexin in liver disease
    Article Snippet: .. De-sialylated glycopeptides were evaporated in a vacuum concentrator (Labconco) and further treated with 0.1 U of 1–3 beta galactosidase (New England BioLabs, Ipswich, MA, USA) in 50 mM sodium acetate, pH 6, at 37 °C for 8 h. Products of the reaction were cleaned for further analysis by SPE C18, concentrated using vacuum concentrator, and dissolved in 0.1 % formic acid for further LC–MS analysis. .. LC–MS/MS analysis Tryptic digest of HPX, HILIC-enriched fractions, or acid- and exoglycosidase-treated glycopeptide fractions were analyzed under identical chromatographic conditions.

    Plasmid Preparation:

    Article Title: Oligomerization of the Tetraspanin CD81 via the Flexibility of Its δ-Loop
    Article Snippet: .. In brief, 105 Jurkat T-cells transfected with a plasmid encoding for CD81-GFP were plated onto poly-lysine-coated coverslips in 1 mL prewarmed Ringer solution (130 mM NaCl, 4 mM KCl, 1 mM CaCl2 , 1 mM MgCl2 , 48 mM D(+)glucose, 10 mM HEPES, pH 7.4) for control samples or prewarmed Ringer solution supplemented with 0.5 unit/mL neuraminidase (#N2876; Sigma-Aldrich, St. Louis, MO) and 4 units/mL β 1-4 galactosidase (#P0730; New England Biolabs, Ipswich, MA) for treated samples. .. Cell suspensions were plated on poly-L-lysine-coated coverslips in six-well plates and incubated for 1 h in a cell incubator.

    Article Title: Ornithine capture by a translating ribosome controls bacterial polyamine synthesis
    Article Snippet: .. β-galactosidase assay To test for in vivo activity, a translational reporter plasmid was obtained by fusing a region containing speFL , the speFL–speF intergenic region and the first three codons of speF to lacZα. .. The insert was prepared by PCR amplification from the E. coli K12 genome using oligonucleotides 7 and 8.

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  • 94
    New England Biolabs β galactosidase assay
    β Galactosidase Assay, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/β galactosidase assay/product/New England Biolabs
    Average 94 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    β galactosidase assay - by Bioz Stars, 2021-02
    94/100 stars
      Buy from Supplier

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