• heparan lyases 1 2 3  (New England Biolabs)


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    Name:
    Bacteroides Heparinase I
    Description:
    Bacteroides Heparinase I 600 units
    Catalog Number:
    p0735l
    Price:
    306
    Size:
    600 units
    Category:
    Glycosidases
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    Structured Review

    New England Biolabs • heparan lyases 1 2 3
    Bacteroides Heparinase I
    Bacteroides Heparinase I 600 units
    https://www.bioz.com/result/• heparan lyases 1 2 3/product/New England Biolabs
    Average 89 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    • heparan lyases 1 2 3 - by Bioz Stars, 2020-01
    89/100 stars

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    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: A Naturally Occurring Polymorphism in the HIV-1 Tat Basic Domain Inhibits Uptake by Bystander Cells and Leads to Reduced Neuroinflammation
    Article Snippet: Antibodies and Enzymes For Tat detection in ELISA, we used a mouse anti-Tat monoclonal antibody (clone E2.1) raised against a 15 residue N-terminal fragment (amino acid residues 1 to 15) of HIV-1C Tat protein (BL43 strain), a gift from Dr. Udaykumar Ranga (Jawaharalal Nehru Centre for Advanced Scientific Research, Bangalore, India), and a rabbit anti-c-Myc polyclonal antibody (clone A14). .. The cell surface HSPGs were digested using Heparinase I and Heparinase III enzymes (both cloned from Bacteroides strains; NE Biolabs #P0735S and #P0737S respectively).

    In Vivo:

    Article Title: Parasite specific 7SL-derived small RNA is an effective target for diagnosis of active trypanosomiasis infection
    Article Snippet: .. In vivo RNA samples from the GALVmed/Clinvet trial were isolated from plasma derived from heparinised blood and therefore required 2 units of Bacteroides Heparinase 1 (New England BioLabs, cat. number: P0735) per RT reaction. .. Subsequent to the RT reaction, a qPCR was performed using a commercial kit (TaqMan universal PCR master mix, Thermo, cat. number: 4304437), according to manufacturer’s instructions.

    In Vitro:

    Article Title: Epigallocatechin-3-gallate remodels apolipoprotein A-I amyloid fibrils into soluble oligomers in the presence of heparin
    Article Snippet: .. Binding of heparin to apoA-I fibrils In vitro binding assays of apoA-I fibril-heparin binding were performed using an adaptation of a procedure described previously , in which the amount of GAG remaining unbound at different fibril/GAG concentration ratios was determined using Bacteroides heparinase I (New England Biolabs Ltd., UK). .. The heparinase enzyme cleaves heparin yielding oligosaccharide products containing unsaturated uronic acids, which can be detected using UV spectroscopy at 232 nm.

    Article Title: Epigallocatechin-3-gallate remodels apolipoprotein A-I amyloid fibrils into soluble oligomers in the presence of heparin
    Article Snippet: .. In vitro binding assays of apoA-I fibril-heparin binding were performed using an adaptation of a procedure described previously , in which the amount of GAG remaining unbound at different fibril/GAG concentration ratios was determined using Bacteroides heparinase I (New England Biolabs Ltd., UK). .. The heparinase enzyme cleaves heparin yielding oligosaccharide products containing unsaturated uronic acids, which can be detected using UV spectroscopy at 232 nm.

    Positive Control:

    Article Title: Circumventing qPCR inhibition to amplify miRNAs in plasma
    Article Snippet: To address this problem, we extracted RNA using the miRNeasy serum/blood plasma kit (Qiagen), which uses phenol and chloroform, and as a positive control, spiked plasma with C. elegans miR-39 mimic (Qiagen) [ ]. .. The first modification included in the approach for processing plasma (Experiment 1, Figure A and B) was replacement of Flavobacterium heparinum heparinase I with Bacteroides heparinase I (New England BioLabs) during RT (Experiment 1, Figure A and B).

    Liquid Chromatography with Mass Spectroscopy:

    Article Title: On-slide tissue digestion for mass spectrometry based glycomic and proteomic profiling
    Article Snippet: Wear gloves and safety goggles while handling • Dithiothreitol (D0632, Sigma), • Iodoacetamide(163-2109, Biorad), • Trifluoro-acetic acid(A116-10X AMP, Fisher Scientific) • acetonitrile(A955-1, LC–MS grade, Fisher Scientific), • Water (W6-1, LC–MS grade, Fisher Scientific), • Formic acid(A117-10x-1AMP, LC–MS grade, Fisher Scientific) CAUTION flammable, corrosive. .. Wear gloves and safety goggles while handling • Hyaluronidase (H1136-1AMP, Sigma) • Chondroitinase ABC(Sigma) • Heparan lyases 1,2,3(P0735 L, P0736 L, P0737 L New England Biolabs) • PNGase F(P0705 L, New England Biolabs) • Trypsin (V528A, Prozyme) • Kasil (C033116, PQ Corporation) • Formamide (F7503, Sigma Aldrich) • Superdex peptide(3.2/300 column, GE Healthcare Life Sciences) • C18 micro-Ziptip(ZTC18M096, Millipore)

    Isolation:

    Article Title: Circumventing qPCR inhibition to amplify miRNAs in plasma
    Article Snippet: However, when RNA isolated using miRNeasy was subjected to qPCR, the Ct values did not improve, including for control C. elegans miR-39 which was employed to spike plasma (Figure A and B). .. The first modification included in the approach for processing plasma (Experiment 1, Figure A and B) was replacement of Flavobacterium heparinum heparinase I with Bacteroides heparinase I (New England BioLabs) during RT (Experiment 1, Figure A and B).

    Article Title: Parasite specific 7SL-derived small RNA is an effective target for diagnosis of active trypanosomiasis infection
    Article Snippet: .. In vivo RNA samples from the GALVmed/Clinvet trial were isolated from plasma derived from heparinised blood and therefore required 2 units of Bacteroides Heparinase 1 (New England BioLabs, cat. number: P0735) per RT reaction. .. Subsequent to the RT reaction, a qPCR was performed using a commercial kit (TaqMan universal PCR master mix, Thermo, cat. number: 4304437), according to manufacturer’s instructions.

    Detection Assay:

    Article Title: Parasite specific 7SL-derived small RNA is an effective target for diagnosis of active trypanosomiasis infection
    Article Snippet: RT-qPCR A species-specific 7SL-derived small RNA stem loop primer-probe detection assay was optimised, using custom primer and probe mixes made by Life Technologies, based on specific sequences (Custom TaqMan Small RNA assay, cat. number: 4398989 [assay IDs T . brucei : CTFVKNM; T . congolense : CTRWEM9; T . vivax : CTDJXGZ]). .. In vivo RNA samples from the GALVmed/Clinvet trial were isolated from plasma derived from heparinised blood and therefore required 2 units of Bacteroides Heparinase 1 (New England BioLabs, cat. number: P0735) per RT reaction.

    Quantitative RT-PCR:

    Article Title: Parasite specific 7SL-derived small RNA is an effective target for diagnosis of active trypanosomiasis infection
    Article Snippet: Paragraph title: RT-qPCR ... In vivo RNA samples from the GALVmed/Clinvet trial were isolated from plasma derived from heparinised blood and therefore required 2 units of Bacteroides Heparinase 1 (New England BioLabs, cat. number: P0735) per RT reaction.

    Real-time Polymerase Chain Reaction:

    Article Title: Circumventing qPCR inhibition to amplify miRNAs in plasma
    Article Snippet: However, when RNA isolated using miRNeasy was subjected to qPCR, the Ct values did not improve, including for control C. elegans miR-39 which was employed to spike plasma (Figure A and B). .. The first modification included in the approach for processing plasma (Experiment 1, Figure A and B) was replacement of Flavobacterium heparinum heparinase I with Bacteroides heparinase I (New England BioLabs) during RT (Experiment 1, Figure A and B).

    Article Title: Parasite specific 7SL-derived small RNA is an effective target for diagnosis of active trypanosomiasis infection
    Article Snippet: In vivo RNA samples from the GALVmed/Clinvet trial were isolated from plasma derived from heparinised blood and therefore required 2 units of Bacteroides Heparinase 1 (New England BioLabs, cat. number: P0735) per RT reaction. .. Subsequent to the RT reaction, a qPCR was performed using a commercial kit (TaqMan universal PCR master mix, Thermo, cat. number: 4304437), according to manufacturer’s instructions.

    Concentration Assay:

    Article Title: Epigallocatechin-3-gallate remodels apolipoprotein A-I amyloid fibrils into soluble oligomers in the presence of heparin
    Article Snippet: .. Binding of heparin to apoA-I fibrils In vitro binding assays of apoA-I fibril-heparin binding were performed using an adaptation of a procedure described previously , in which the amount of GAG remaining unbound at different fibril/GAG concentration ratios was determined using Bacteroides heparinase I (New England Biolabs Ltd., UK). .. The heparinase enzyme cleaves heparin yielding oligosaccharide products containing unsaturated uronic acids, which can be detected using UV spectroscopy at 232 nm.

    Article Title: Epigallocatechin-3-gallate remodels apolipoprotein A-I amyloid fibrils into soluble oligomers in the presence of heparin
    Article Snippet: .. In vitro binding assays of apoA-I fibril-heparin binding were performed using an adaptation of a procedure described previously , in which the amount of GAG remaining unbound at different fibril/GAG concentration ratios was determined using Bacteroides heparinase I (New England Biolabs Ltd., UK). .. The heparinase enzyme cleaves heparin yielding oligosaccharide products containing unsaturated uronic acids, which can be detected using UV spectroscopy at 232 nm.

    Article Title: Circumventing qPCR inhibition to amplify miRNAs in plasma
    Article Snippet: .. Here Ct values were inversely related to the concentration of Bacteroides heparinase I (Figure B). .. Moreover, using 12 U Bacteroides heparinase I with 3 μl and 6 μl RNA resulted in miRTC values that were ≤ 5 cycles less than the positive qPCR controls (PPC) (Figure B, F, G and H).

    Incubation:

    Article Title: C-Terminal Amino Acids 471-507 of Avian Hepatitis E Virus Capsid Protein Are Crucial for Binding to Avian and Human Cells
    Article Snippet: Heparinase II treatment of LMH cells LMH cells were pre-treated in Heparinase Reaction Buffer (20 mM Tris-HCl, 100 mM NaCl and 1.5 mM CaCl2 ) with heparinase II (10U/ml) from Bacteroides (New England Biolabs GmbH, Frankfurt am Main, Germany) or PBS as control for 2h at 37°C in an atmosphere supplied with 5% CO2 . .. After 2h of incubation cells were washed with PBS and incubated with ORF2-3 or ORF2-4 (500nM each) for 1h at 37°C with 5% CO2 .

    Article Title: Heparin affinity purification of extracellular vesicles
    Article Snippet: .. Beads were then washed three times with PBS 1x and either treated with Bacteroides Heparinase I (New England Biolabs, Ipswich, MA; 60U/ml heparin beads) according to the manufacturer’s recommendations or mock treated (incubation buffer devoid of heparinase) for 1 hr at 30 °C. ..

    Article Title: Circumventing qPCR inhibition to amplify miRNAs in plasma
    Article Snippet: The first modification included in the approach for processing plasma (Experiment 1, Figure A and B) was replacement of Flavobacterium heparinum heparinase I with Bacteroides heparinase I (New England BioLabs) during RT (Experiment 1, Figure A and B). .. For pre-treatment of the RNA before RT [ , ], 0.6 U and 6.0 U Bacteroides heparinase I were incubated with 3 μl RNA for 2 h at 25°C then subsequently reverse transcribed.

    other:

    Article Title: Heparin Promotes Cardiac Differentiation of Human Pluripotent Stem Cells in Chemically Defined Albumin‐Free Medium, Enabling Consistent Manufacture of Cardiomyocytes
    Article Snippet: The growth factors, inhibitors, and enzymes used in culture were as follows: activin A (10 ng/ml, catalog no. 338‐AC/CF; R & D Systems, Minneapolis, MN, https://www.rndsystems.com ), BMP4 (10 ng/ml, catalog no. 314‐BP/CF; R & D Systems), fibroblast growth factor 2 (FGF2; 100 ng/ml, catalog no. 100‐18B; Peprotech, Rocky Hill, NJ, https://www.peprotech.com ), transforming growth factor β (TGFβ) 1 (1.74 ng/ml, catalog no. 240‐B/CF; R & D), dorsomorphin (3 μM, catalog no. 04‐0024; Stemgent, Lexington, MA, https://www.stemgent.com ), LDN‐193189 (0.1 μM, catalog no. 04‐0074; Stemgent), PD0325901 (1 μM, catalog no. 04‐0008; Stemgent), SB431542 (3 μM, catalog no. s1067; Selleckchem, Houston, TX, http://www.selleckchem.com ), heparin (catalog no. H3149; Sigma‐Aldrich), heparinase I (catalog no. P0735S; New England Biolabs, Ipswich, MA, https://www.neb.com ).

    Spectroscopy:

    Article Title: Epigallocatechin-3-gallate remodels apolipoprotein A-I amyloid fibrils into soluble oligomers in the presence of heparin
    Article Snippet: Binding of heparin to apoA-I fibrils In vitro binding assays of apoA-I fibril-heparin binding were performed using an adaptation of a procedure described previously , in which the amount of GAG remaining unbound at different fibril/GAG concentration ratios was determined using Bacteroides heparinase I (New England Biolabs Ltd., UK). .. The heparinase enzyme cleaves heparin yielding oligosaccharide products containing unsaturated uronic acids, which can be detected using UV spectroscopy at 232 nm.

    Article Title: Epigallocatechin-3-gallate remodels apolipoprotein A-I amyloid fibrils into soluble oligomers in the presence of heparin
    Article Snippet: In vitro binding assays of apoA-I fibril-heparin binding were performed using an adaptation of a procedure described previously , in which the amount of GAG remaining unbound at different fibril/GAG concentration ratios was determined using Bacteroides heparinase I (New England Biolabs Ltd., UK). .. The heparinase enzyme cleaves heparin yielding oligosaccharide products containing unsaturated uronic acids, which can be detected using UV spectroscopy at 232 nm.

    Modification:

    Article Title: Circumventing qPCR inhibition to amplify miRNAs in plasma
    Article Snippet: .. The first modification included in the approach for processing plasma (Experiment 1, Figure A and B) was replacement of Flavobacterium heparinum heparinase I with Bacteroides heparinase I (New England BioLabs) during RT (Experiment 1, Figure A and B). ..

    Polymerase Chain Reaction:

    Article Title: Parasite specific 7SL-derived small RNA is an effective target for diagnosis of active trypanosomiasis infection
    Article Snippet: In vivo RNA samples from the GALVmed/Clinvet trial were isolated from plasma derived from heparinised blood and therefore required 2 units of Bacteroides Heparinase 1 (New England BioLabs, cat. number: P0735) per RT reaction. .. Subsequent to the RT reaction, a qPCR was performed using a commercial kit (TaqMan universal PCR master mix, Thermo, cat. number: 4304437), according to manufacturer’s instructions.

    Staining:

    Article Title: C-Terminal Amino Acids 471-507 of Avian Hepatitis E Virus Capsid Protein Are Crucial for Binding to Avian and Human Cells
    Article Snippet: Heparinase II treatment of LMH cells LMH cells were pre-treated in Heparinase Reaction Buffer (20 mM Tris-HCl, 100 mM NaCl and 1.5 mM CaCl2 ) with heparinase II (10U/ml) from Bacteroides (New England Biolabs GmbH, Frankfurt am Main, Germany) or PBS as control for 2h at 37°C in an atmosphere supplied with 5% CO2 . .. Samples were analyzed by immunofluorescence staining as described for the experiments with heparin sodium salt.

    Binding Assay:

    Article Title: Epigallocatechin-3-gallate remodels apolipoprotein A-I amyloid fibrils into soluble oligomers in the presence of heparin
    Article Snippet: .. Binding of heparin to apoA-I fibrils In vitro binding assays of apoA-I fibril-heparin binding were performed using an adaptation of a procedure described previously , in which the amount of GAG remaining unbound at different fibril/GAG concentration ratios was determined using Bacteroides heparinase I (New England Biolabs Ltd., UK). .. The heparinase enzyme cleaves heparin yielding oligosaccharide products containing unsaturated uronic acids, which can be detected using UV spectroscopy at 232 nm.

    Article Title: Epigallocatechin-3-gallate remodels apolipoprotein A-I amyloid fibrils into soluble oligomers in the presence of heparin
    Article Snippet: .. In vitro binding assays of apoA-I fibril-heparin binding were performed using an adaptation of a procedure described previously , in which the amount of GAG remaining unbound at different fibril/GAG concentration ratios was determined using Bacteroides heparinase I (New England Biolabs Ltd., UK). .. The heparinase enzyme cleaves heparin yielding oligosaccharide products containing unsaturated uronic acids, which can be detected using UV spectroscopy at 232 nm.

    Immunofluorescence:

    Article Title: C-Terminal Amino Acids 471-507 of Avian Hepatitis E Virus Capsid Protein Are Crucial for Binding to Avian and Human Cells
    Article Snippet: Heparinase II treatment of LMH cells LMH cells were pre-treated in Heparinase Reaction Buffer (20 mM Tris-HCl, 100 mM NaCl and 1.5 mM CaCl2 ) with heparinase II (10U/ml) from Bacteroides (New England Biolabs GmbH, Frankfurt am Main, Germany) or PBS as control for 2h at 37°C in an atmosphere supplied with 5% CO2 . .. Samples were analyzed by immunofluorescence staining as described for the experiments with heparin sodium salt.

    Derivative Assay:

    Article Title: Circumventing qPCR inhibition to amplify miRNAs in plasma
    Article Snippet: The yield of RNA derived from plasma in both the QIAamp and miRNAeasy methods was 8-12 ng/μl, and was of similar quality to that we have recovered from sera [ ], although RNA from plasma exhibited lower 260/230 ratios, ( < 0.7) with the miRNeasy kit. .. The first modification included in the approach for processing plasma (Experiment 1, Figure A and B) was replacement of Flavobacterium heparinum heparinase I with Bacteroides heparinase I (New England BioLabs) during RT (Experiment 1, Figure A and B).

    Article Title: Parasite specific 7SL-derived small RNA is an effective target for diagnosis of active trypanosomiasis infection
    Article Snippet: .. In vivo RNA samples from the GALVmed/Clinvet trial were isolated from plasma derived from heparinised blood and therefore required 2 units of Bacteroides Heparinase 1 (New England BioLabs, cat. number: P0735) per RT reaction. .. Subsequent to the RT reaction, a qPCR was performed using a commercial kit (TaqMan universal PCR master mix, Thermo, cat. number: 4304437), according to manufacturer’s instructions.

    TaqMan Assay:

    Article Title: Parasite specific 7SL-derived small RNA is an effective target for diagnosis of active trypanosomiasis infection
    Article Snippet: Reverse transcription was carried out using a commercial cDNA Reverse Transcription Kit (Applied Biosciences, cat. number: 4368814), replacing the random primers with the aforementioned TaqMan assay primer. .. In vivo RNA samples from the GALVmed/Clinvet trial were isolated from plasma derived from heparinised blood and therefore required 2 units of Bacteroides Heparinase 1 (New England BioLabs, cat. number: P0735) per RT reaction.

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  • 89
    New England Biolabs • heparan lyases 1 2 3
    • Heparan Lyases 1 2 3, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/• heparan lyases 1 2 3/product/New England Biolabs
    Average 89 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    • heparan lyases 1 2 3 - by Bioz Stars, 2020-01
    89/100 stars
      Buy from Supplier

    85
    New England Biolabs bacteroides heparinase ii
    LDL binding to the vessel wall in vitro following treatment with Site B peptide or enzymatic digestion of proteoglycan GAG chains. Tissue sections of carotid arteries from wild‐type mice with intimal hyperplasia were incubated with human LDL . Bound LDL was detected using anti‐apoB antibody. (A) LDL binding to tissue sections pre‐incubated with positively charged SiteB peptide (white circles) or neutrally charged SiteB KE peptide (gray circles). Black circles=no pre‐treatment. White triangles=no LDL incubation (buffer only). (B) LDL binding to tissue sections pre‐treated with the GAG ‐degrading enzymes chondroitinase (white circles) or <t>heparinase</t> (white dot circles). Black circles=no pre‐treatment. White triangles=no LDL incubation (buffer only). Data was analyzed using Mann–Whitney rank sum test. Graph bars show median values. P
    Bacteroides Heparinase Ii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bacteroides heparinase ii/product/New England Biolabs
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bacteroides heparinase ii - by Bioz Stars, 2020-01
    85/100 stars
      Buy from Supplier

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    LDL binding to the vessel wall in vitro following treatment with Site B peptide or enzymatic digestion of proteoglycan GAG chains. Tissue sections of carotid arteries from wild‐type mice with intimal hyperplasia were incubated with human LDL . Bound LDL was detected using anti‐apoB antibody. (A) LDL binding to tissue sections pre‐incubated with positively charged SiteB peptide (white circles) or neutrally charged SiteB KE peptide (gray circles). Black circles=no pre‐treatment. White triangles=no LDL incubation (buffer only). (B) LDL binding to tissue sections pre‐treated with the GAG ‐degrading enzymes chondroitinase (white circles) or heparinase (white dot circles). Black circles=no pre‐treatment. White triangles=no LDL incubation (buffer only). Data was analyzed using Mann–Whitney rank sum test. Graph bars show median values. P

    Journal: Physiological Reports

    Article Title: Intimal hyperplasia induced by vascular intervention causes lipoprotein retention and accelerated atherosclerosis. Intimal hyperplasia induced by vascular intervention causes lipoprotein retention and accelerated atherosclerosis

    doi: 10.14814/phy2.13334

    Figure Lengend Snippet: LDL binding to the vessel wall in vitro following treatment with Site B peptide or enzymatic digestion of proteoglycan GAG chains. Tissue sections of carotid arteries from wild‐type mice with intimal hyperplasia were incubated with human LDL . Bound LDL was detected using anti‐apoB antibody. (A) LDL binding to tissue sections pre‐incubated with positively charged SiteB peptide (white circles) or neutrally charged SiteB KE peptide (gray circles). Black circles=no pre‐treatment. White triangles=no LDL incubation (buffer only). (B) LDL binding to tissue sections pre‐treated with the GAG ‐degrading enzymes chondroitinase (white circles) or heparinase (white dot circles). Black circles=no pre‐treatment. White triangles=no LDL incubation (buffer only). Data was analyzed using Mann–Whitney rank sum test. Graph bars show median values. P

    Article Snippet: For GAG enzyme experiments, tissue sections were pre‐incubated 30 min at RT with Heparinase II (New England BioLabs, P0736S) or Chondrotinase ABC (Sigma, C3667) as per manufacturer's recommendations.

    Techniques: Binding Assay, In Vitro, Mouse Assay, Incubation, MANN-WHITNEY