α n acetylgalactosaminidase  (New England Biolabs)


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    Name:
    alpha N Acetyl galactosaminidase
    Description:
    alpha N Acetyl galactosaminidase 15 000 units
    Catalog Number:
    p0734l
    Price:
    539
    Size:
    15 000 units
    Category:
    Glycosidases
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    Structured Review

    New England Biolabs α n acetylgalactosaminidase
    alpha N Acetyl galactosaminidase
    alpha N Acetyl galactosaminidase 15 000 units
    https://www.bioz.com/result/α n acetylgalactosaminidase/product/New England Biolabs
    Average 93 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    α n acetylgalactosaminidase - by Bioz Stars, 2021-01
    93/100 stars

    Images

    1) Product Images from "The Galectin CvGal1 from the Eastern Oyster (Crassostrea virginica) Binds to Blood Group A Oligosaccharides on the Hemocyte Surface *"

    Article Title: The Galectin CvGal1 from the Eastern Oyster (Crassostrea virginica) Binds to Blood Group A Oligosaccharides on the Hemocyte Surface *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M113.476531

    Binding of rCvGal1 to intact and glycosidase-treated hemocytes. Binding of rCvGal1 ( A ) or anti-blood group A antibody ( B ) to unattached hemocytes with α- N -acetylgalactosaminidase treatment ( GalNAc'ase , blue line ) or no treatment ( Untreated , red
    Figure Legend Snippet: Binding of rCvGal1 to intact and glycosidase-treated hemocytes. Binding of rCvGal1 ( A ) or anti-blood group A antibody ( B ) to unattached hemocytes with α- N -acetylgalactosaminidase treatment ( GalNAc'ase , blue line ) or no treatment ( Untreated , red

    Techniques Used: Binding Assay

    2) Product Images from "Annotation and structural elucidation of bovine milk oligosaccharides and determination of novel fucosylated structures"

    Article Title: Annotation and structural elucidation of bovine milk oligosaccharides and determination of novel fucosylated structures

    Journal: Glycobiology

    doi: 10.1093/glycob/cwt007

    ( A ) EIC of m/z 548.2 from the BMO pool showing two main isomers. ( B ) EIC of m/z 548.2 after digestion with α N -acetylgalactosaminidase. ( C ) EIC of m/z 548.2 after digestion with β N -acetylhexosaminidase. ( D ) Magnification on base line
    Figure Legend Snippet: ( A ) EIC of m/z 548.2 from the BMO pool showing two main isomers. ( B ) EIC of m/z 548.2 after digestion with α N -acetylgalactosaminidase. ( C ) EIC of m/z 548.2 after digestion with β N -acetylhexosaminidase. ( D ) Magnification on base line

    Techniques Used:

    Related Articles

    Recombinant:

    Article Title: Annotation and structural elucidation of bovine milk oligosaccharides and determination of novel fucosylated structures
    Article Snippet: .. Recombinant β1-3,6 galactosidase, α1-3,6 galactosidase, β N -acetylhexosaminidase, α N -acetylgalactosaminidase, β N -acetylhexosaminidase, β N -acetylglucosaminidase and α2-3 neuraminidase were purchased from New England Biolabs (Ispwich, MA). β1-4 galactosidase was purchased from Prozyme (Hayward, CA). .. All other reagents were of the analytical or HPLC grade.

    Purification:

    Article Title: Biochemical and functional characterization of CpMuc4, aCryptosporidium surface antigen that binds to host epithelial cells
    Article Snippet: .. Purified nCpMuc4 was digested with peptide -N-glycosidase F (PNGase-F),α -fucosidase , α N-acetyl galactosaminidase, and a proteindeglycosylation mixture consisting of PNGase-F, O-glycosidase, neuraminidase,β1-4 galactosidase, and β-N-acetylglucosaminidase (all enzymes andProtein Deglycosylation Mix from New England Biolabs (NEB), Ipswich, MA).nCpMuc4 incubated in the digestion buffers in the absence of the enzymes was runin parallel as a control. .. The activity of the enzymes in the ProteinDeglycosylation Mix was confirmed by digestion of fetuin, an N- andO-glycosylated protein included in the protein deglycosylation kit as a positivecontrol (NEB).

    Concentration Assay:

    Article Title: Enterotoxigenic Escherichia coli–blood group A interactions intensify diarrheal severity
    Article Snippet: .. To examine the specificity of EtpA-induced agglutination of RBCs expressing the A antigen, agglutination assays were performed in the presence of excess terminal sugars specific to individual blood types: GalNAc (blood group A), Gal (blood group B), or control GlcNAc at 50 mM final concentration. α- N -acetylgalactosaminidase (P0734S; New England Biolabs) was used to remove terminal GalNAc from the A blood group antigen by incubating 50 μl of a 1% RBC suspension with 20 U α- N -acetylgalactosaminidase for 4 hours at 37°C. ..

    Article Title: Histo-Blood Group Antigen-Like Substances of Human Enteric Bacteria as Specific Adsorbents for Human Noroviruses
    Article Snippet: .. One hundred microliters of reaction mixture contained 88 μl of extracted EPS, 10 μl of 10× G7 reaction buffer, and 1 μl of α- N -acetyl-galactosaminidase to yield a final concentration of 0.2 U/ml (New England BioLabs, Inc., Japan), and 1 μl of 10 mg/ml BSA. ..

    Incubation:

    Article Title: Biochemical and functional characterization of CpMuc4, aCryptosporidium surface antigen that binds to host epithelial cells
    Article Snippet: .. Purified nCpMuc4 was digested with peptide -N-glycosidase F (PNGase-F),α -fucosidase , α N-acetyl galactosaminidase, and a proteindeglycosylation mixture consisting of PNGase-F, O-glycosidase, neuraminidase,β1-4 galactosidase, and β-N-acetylglucosaminidase (all enzymes andProtein Deglycosylation Mix from New England Biolabs (NEB), Ipswich, MA).nCpMuc4 incubated in the digestion buffers in the absence of the enzymes was runin parallel as a control. .. The activity of the enzymes in the ProteinDeglycosylation Mix was confirmed by digestion of fetuin, an N- andO-glycosylated protein included in the protein deglycosylation kit as a positivecontrol (NEB).

    other:

    Article Title: Bovine Nebovirus Interacts with a Wide Spectrum of Histo-Blood Group Antigens
    Article Snippet: NaIO4 and NA, from Sigma-Aldrich, were dissolved in phosphate-buffered saline (PBS; pH 7.2). α1,2-l -Fucosidase from Corynebacterium (TaKaRa Bio Inc., Kyoto, Japan), α1,3/4-l -fucosidase from Streptomyces (TaKaRa Bio Inc.), α-galactosidase from Coffea arabica (Sigma-Aldrich), α-N -acetylgalactosaminidase from Chryseobacterium meningosepticum (New England BioLabs, Inc., MA, USA), and UEA-1 (Vector Laboratories, Burlingame, CA, USA) were diluted in PBS.

    Expressing:

    Article Title: Enterotoxigenic Escherichia coli–blood group A interactions intensify diarrheal severity
    Article Snippet: .. To examine the specificity of EtpA-induced agglutination of RBCs expressing the A antigen, agglutination assays were performed in the presence of excess terminal sugars specific to individual blood types: GalNAc (blood group A), Gal (blood group B), or control GlcNAc at 50 mM final concentration. α- N -acetylgalactosaminidase (P0734S; New England Biolabs) was used to remove terminal GalNAc from the A blood group antigen by incubating 50 μl of a 1% RBC suspension with 20 U α- N -acetylgalactosaminidase for 4 hours at 37°C. ..

    Agglutination:

    Article Title: Enterotoxigenic Escherichia coli–blood group A interactions intensify diarrheal severity
    Article Snippet: .. To examine the specificity of EtpA-induced agglutination of RBCs expressing the A antigen, agglutination assays were performed in the presence of excess terminal sugars specific to individual blood types: GalNAc (blood group A), Gal (blood group B), or control GlcNAc at 50 mM final concentration. α- N -acetylgalactosaminidase (P0734S; New England Biolabs) was used to remove terminal GalNAc from the A blood group antigen by incubating 50 μl of a 1% RBC suspension with 20 U α- N -acetylgalactosaminidase for 4 hours at 37°C. ..

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  • 93
    New England Biolabs α n acetylgalactosaminidase
    Binding of rCvGal1 to intact and glycosidase-treated hemocytes. Binding of rCvGal1 ( A ) or anti-blood group A antibody ( B ) to unattached hemocytes with α- N <t>-acetylgalactosaminidase</t> treatment ( GalNAc'ase , blue line ) or no treatment ( Untreated , red
    α N Acetylgalactosaminidase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/α n acetylgalactosaminidase/product/New England Biolabs
    Average 93 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    α n acetylgalactosaminidase - by Bioz Stars, 2021-01
    93/100 stars
      Buy from Supplier

    Image Search Results


    Binding of rCvGal1 to intact and glycosidase-treated hemocytes. Binding of rCvGal1 ( A ) or anti-blood group A antibody ( B ) to unattached hemocytes with α- N -acetylgalactosaminidase treatment ( GalNAc'ase , blue line ) or no treatment ( Untreated , red

    Journal: The Journal of Biological Chemistry

    Article Title: The Galectin CvGal1 from the Eastern Oyster (Crassostrea virginica) Binds to Blood Group A Oligosaccharides on the Hemocyte Surface *

    doi: 10.1074/jbc.M113.476531

    Figure Lengend Snippet: Binding of rCvGal1 to intact and glycosidase-treated hemocytes. Binding of rCvGal1 ( A ) or anti-blood group A antibody ( B ) to unattached hemocytes with α- N -acetylgalactosaminidase treatment ( GalNAc'ase , blue line ) or no treatment ( Untreated , red

    Article Snippet: PNGase F (from Flavobacterium meningosepticum ), O -glycosidase (cloned from Enterococcus faecalis and expressed in Escherichia coli ), α- N -acetylgalactosaminidase (cloned from Chryseobacterium meningosepticum and expressed in E. coli ), and β1,3-galactosidase (cloned from Xanthomonas manihotis and expressed in E. coli ) were obtained from New England Biolabs (Ipswich, MA).

    Techniques: Binding Assay

    ( A ) EIC of m/z 548.2 from the BMO pool showing two main isomers. ( B ) EIC of m/z 548.2 after digestion with α N -acetylgalactosaminidase. ( C ) EIC of m/z 548.2 after digestion with β N -acetylhexosaminidase. ( D ) Magnification on base line

    Journal: Glycobiology

    Article Title: Annotation and structural elucidation of bovine milk oligosaccharides and determination of novel fucosylated structures

    doi: 10.1093/glycob/cwt007

    Figure Lengend Snippet: ( A ) EIC of m/z 548.2 from the BMO pool showing two main isomers. ( B ) EIC of m/z 548.2 after digestion with α N -acetylgalactosaminidase. ( C ) EIC of m/z 548.2 after digestion with β N -acetylhexosaminidase. ( D ) Magnification on base line

    Article Snippet: Recombinant β1-3,6 galactosidase, α1-3,6 galactosidase, β N -acetylhexosaminidase, α N -acetylgalactosaminidase, β N -acetylhexosaminidase, β N -acetylglucosaminidase and α2-3 neuraminidase were purchased from New England Biolabs (Ispwich, MA). β1-4 galactosidase was purchased from Prozyme (Hayward, CA).

    Techniques: