sialic acid  (New England Biolabs)


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  • 93
    Name:
    beta 1 3 Galactosidase
    Description:
    beta 1 3 Galactosidase 2 500 units
    Catalog Number:
    p0726l
    Price:
    518
    Size:
    2 500 units
    Category:
    Glycosidases
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    Structured Review

    New England Biolabs sialic acid
    beta 1 3 Galactosidase
    beta 1 3 Galactosidase 2 500 units
    https://www.bioz.com/result/sialic acid/product/New England Biolabs
    Average 93 stars, based on 137 article reviews
    Price from $9.99 to $1999.99
    sialic acid - by Bioz Stars, 2020-07
    93/100 stars

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    Related Articles

    Mass Spectrometry:

    Article Title: Isomeric Separation and Recognition of Anionic and Zwitterionic N-glycans from Royal Jelly Glycoproteins
    Article Snippet: .. Glycans were treated, before re-analysis by MALDI-TOF MS, with α-fucosidase (bovine kidney from Sigma-Aldrich), α-mannosidases (jack bean from Sigma-Aldrich, Aspergillus α1,2-specific from Prozyme, Hayward, CA, and Xanthomonas α1,2/3-specific from New England Biolabs), β-galactosidase (β1,3-specific from New England Biolabs), β-glucuronidases ( E. coli from Megazyme, Bray, Ireland, and Helix pomatia from Sigma-Aldrich; desalted and concentrated before use), β- N -acetylhexosaminidases (jack bean from Sigma-Aldrich, Xanthomonas β1,2-specific N -acetylglucosaminidase from New England Biolabs, Streptomyces β1,3/4-specific N -acetylhexosaminidase (chitinase) from New England Biolabs or in-house produced recombinant forms of Caenorhabditis elegans HEX-4 specific for β1,4-linked GalNAc residues or Apis mellifera FDL specific for the product of GlcNAc-transferase I ( )) in 50 m m ammonium acetate, pH 5, at 37 °C overnight (except for pH 6.5 in the case of HEX-4, pH 7 in the case of E. coli β-glucuronidase or an incubation time of only 3 h in the case of FDL); these incubations were performed in PCR tubes with a final volume of 3 μl (for further details about conditions and specificities, refer to the supplement). ..

    Produced:

    Article Title: Isomeric Separation and Recognition of Anionic and Zwitterionic N-glycans from Royal Jelly Glycoproteins
    Article Snippet: .. Glycans were treated, before re-analysis by MALDI-TOF MS, with α-fucosidase (bovine kidney from Sigma-Aldrich), α-mannosidases (jack bean from Sigma-Aldrich, Aspergillus α1,2-specific from Prozyme, Hayward, CA, and Xanthomonas α1,2/3-specific from New England Biolabs), β-galactosidase (β1,3-specific from New England Biolabs), β-glucuronidases ( E. coli from Megazyme, Bray, Ireland, and Helix pomatia from Sigma-Aldrich; desalted and concentrated before use), β- N -acetylhexosaminidases (jack bean from Sigma-Aldrich, Xanthomonas β1,2-specific N -acetylglucosaminidase from New England Biolabs, Streptomyces β1,3/4-specific N -acetylhexosaminidase (chitinase) from New England Biolabs or in-house produced recombinant forms of Caenorhabditis elegans HEX-4 specific for β1,4-linked GalNAc residues or Apis mellifera FDL specific for the product of GlcNAc-transferase I ( )) in 50 m m ammonium acetate, pH 5, at 37 °C overnight (except for pH 6.5 in the case of HEX-4, pH 7 in the case of E. coli β-glucuronidase or an incubation time of only 3 h in the case of FDL); these incubations were performed in PCR tubes with a final volume of 3 μl (for further details about conditions and specificities, refer to the supplement). ..

    Incubation:

    Article Title: Isomeric Separation and Recognition of Anionic and Zwitterionic N-glycans from Royal Jelly Glycoproteins
    Article Snippet: .. Glycans were treated, before re-analysis by MALDI-TOF MS, with α-fucosidase (bovine kidney from Sigma-Aldrich), α-mannosidases (jack bean from Sigma-Aldrich, Aspergillus α1,2-specific from Prozyme, Hayward, CA, and Xanthomonas α1,2/3-specific from New England Biolabs), β-galactosidase (β1,3-specific from New England Biolabs), β-glucuronidases ( E. coli from Megazyme, Bray, Ireland, and Helix pomatia from Sigma-Aldrich; desalted and concentrated before use), β- N -acetylhexosaminidases (jack bean from Sigma-Aldrich, Xanthomonas β1,2-specific N -acetylglucosaminidase from New England Biolabs, Streptomyces β1,3/4-specific N -acetylhexosaminidase (chitinase) from New England Biolabs or in-house produced recombinant forms of Caenorhabditis elegans HEX-4 specific for β1,4-linked GalNAc residues or Apis mellifera FDL specific for the product of GlcNAc-transferase I ( )) in 50 m m ammonium acetate, pH 5, at 37 °C overnight (except for pH 6.5 in the case of HEX-4, pH 7 in the case of E. coli β-glucuronidase or an incubation time of only 3 h in the case of FDL); these incubations were performed in PCR tubes with a final volume of 3 μl (for further details about conditions and specificities, refer to the supplement). ..

    Article Title: How to Dig Deeper? Improved Enrichment Methods for Mucin Core-1 Type Glycopeptides *
    Article Snippet: .. Sialic acid and β-galactose units of glycopeptides were removed by incubation with neuraminidase (5–10 U/sample, New England Biolabs P0720; in 100 m m sodium citrate, pH 6.0) for 1 h at 37 °C followed by overnight treatment with β-galactosidase (10 U/sample, New England Biolabs P0726; in 100 m m sodium citrate, pH 4.5) at 37 °C. .. Enzymatic deglycosylation was stopped by acidification to pH ≤3 with 10% trifluoroacetic acid solution, and the resulting peptide mixtures were desalted on 10 μl C-18 tips (Millipore ZTC18S960).

    Article Title: Deciphering Protein Glycosylation by Computational Integration of On-chip Profiling, Glycan-array Data, and Mass Spectrometry *
    Article Snippet: .. We prepared the β1–3 galactosidase (P0726S, New England Biolabs) or β1–4 galactosidase (GKX-5014, Prozyme, Hayward, CA) enzymes at a 1:100 dilution in the supplied reaction buffers and incubated them separately on arrays overnight at 37 °C. ..

    Liquid Chromatography with Mass Spectroscopy:

    Article Title: Increased sialylation of site specific O-glycoforms of hemopexin in liver disease
    Article Snippet: .. De-sialylated glycopeptides were evaporated in a vacuum concentrator (Labconco) and further treated with 0.1 U of 1–3 beta galactosidase (New England BioLabs, Ipswich, MA, USA) in 50 mM sodium acetate, pH 6, at 37 °C for 8 h. Products of the reaction were cleaned for further analysis by SPE C18, concentrated using vacuum concentrator, and dissolved in 0.1 % formic acid for further LC–MS analysis. .. LC–MS/MS analysis Tryptic digest of HPX, HILIC-enriched fractions, or acid- and exoglycosidase-treated glycopeptide fractions were analyzed under identical chromatographic conditions.

    Polymerase Chain Reaction:

    Article Title: Isomeric Separation and Recognition of Anionic and Zwitterionic N-glycans from Royal Jelly Glycoproteins
    Article Snippet: .. Glycans were treated, before re-analysis by MALDI-TOF MS, with α-fucosidase (bovine kidney from Sigma-Aldrich), α-mannosidases (jack bean from Sigma-Aldrich, Aspergillus α1,2-specific from Prozyme, Hayward, CA, and Xanthomonas α1,2/3-specific from New England Biolabs), β-galactosidase (β1,3-specific from New England Biolabs), β-glucuronidases ( E. coli from Megazyme, Bray, Ireland, and Helix pomatia from Sigma-Aldrich; desalted and concentrated before use), β- N -acetylhexosaminidases (jack bean from Sigma-Aldrich, Xanthomonas β1,2-specific N -acetylglucosaminidase from New England Biolabs, Streptomyces β1,3/4-specific N -acetylhexosaminidase (chitinase) from New England Biolabs or in-house produced recombinant forms of Caenorhabditis elegans HEX-4 specific for β1,4-linked GalNAc residues or Apis mellifera FDL specific for the product of GlcNAc-transferase I ( )) in 50 m m ammonium acetate, pH 5, at 37 °C overnight (except for pH 6.5 in the case of HEX-4, pH 7 in the case of E. coli β-glucuronidase or an incubation time of only 3 h in the case of FDL); these incubations were performed in PCR tubes with a final volume of 3 μl (for further details about conditions and specificities, refer to the supplement). ..

    Recombinant:

    Article Title: Isomeric Separation and Recognition of Anionic and Zwitterionic N-glycans from Royal Jelly Glycoproteins
    Article Snippet: .. Glycans were treated, before re-analysis by MALDI-TOF MS, with α-fucosidase (bovine kidney from Sigma-Aldrich), α-mannosidases (jack bean from Sigma-Aldrich, Aspergillus α1,2-specific from Prozyme, Hayward, CA, and Xanthomonas α1,2/3-specific from New England Biolabs), β-galactosidase (β1,3-specific from New England Biolabs), β-glucuronidases ( E. coli from Megazyme, Bray, Ireland, and Helix pomatia from Sigma-Aldrich; desalted and concentrated before use), β- N -acetylhexosaminidases (jack bean from Sigma-Aldrich, Xanthomonas β1,2-specific N -acetylglucosaminidase from New England Biolabs, Streptomyces β1,3/4-specific N -acetylhexosaminidase (chitinase) from New England Biolabs or in-house produced recombinant forms of Caenorhabditis elegans HEX-4 specific for β1,4-linked GalNAc residues or Apis mellifera FDL specific for the product of GlcNAc-transferase I ( )) in 50 m m ammonium acetate, pH 5, at 37 °C overnight (except for pH 6.5 in the case of HEX-4, pH 7 in the case of E. coli β-glucuronidase or an incubation time of only 3 h in the case of FDL); these incubations were performed in PCR tubes with a final volume of 3 μl (for further details about conditions and specificities, refer to the supplement). ..

    Article Title: Novel structural features of the immunocompetent ceramide phospho-inositol glycan core from Trichomonas vaginalis
    Article Snippet: .. In some cases CPI-GC was treated with either β-galactosidase [EC 3.2.1.23] from recombinant clone from Bacteroides fragilis (obtained from New England Bio Labs) alone or in combination with β-N-acetyl hexosaminidase for 24 h in buffer supplied by the manufacturer. ..

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  • 94
    New England Biolabs β galactosidase
    β Galactosidase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/β galactosidase/product/New England Biolabs
    Average 94 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    β galactosidase - by Bioz Stars, 2020-07
    94/100 stars
      Buy from Supplier

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