Journal: Clinical proteomics

Article Title: Analysis of serum protein glycosylation by a differential lectin immunosorbant assay (dLISA)

doi: 10.1186/1559-0275-10-12

Figure Lengend Snippet: Slopes of the dose response curves established using the dLISA approach correlated with TIMP-1 fucosylation. (A) Dose–response curves of serum samples spiked with differentially fucosylated recombinant TIMP-1. Recombinant TIMP-1 was treated by α1, 2 fucosidase at different length of time (0, 15, 30, and 45 minutes) before they were spiked into with four serum aliquots. These aliquots came from the same pool of sera with endogenous TIMP-1 immuno-depleted (confirmed by the TIMP-1 immunoassay). After the recombinant protein spiked in, these serum samples underwent the dLISA approach. (B) A graph presentation of linear regression slopes of the dose–response curves versus the length of fucosidase treatment (minutes).

Article Snippet: Four microliter of the recombinant TIMP-1 (10 μg/mL prepared in PBS + 1% BSA buffer) was mixed with 4 μL of α1, 2 fucosidase (Catalog# P0724, New England Biolabs, Ipswich, MA).

Techniques: Recombinant

Journal: Scientific Reports

Article Title: Analysis of site and structure specific core fucosylation in liver cirrhosis using exoglycosidase-assisted data-independent LC-MS/MS

doi: 10.1038/s41598-021-02838-3

Figure Lengend Snippet: XIC chromatogram of six glycoforms of the SWPAVGNCSSALR glycopeptide of hemopexin extracted from the GP-SWATH DIA data of a MARS14 depleted serum sample treated with α2-3,6,8,9 neuraminidase, α1-2 and α1-3,4 fucosidases.

Article Snippet: Trypsin Gold (V5280) was obtained from Promega (Madison, WI). α2-3,6,8,9 neuraminidase (P0722), α1-2 (P0724) and α1-3,4 (P0769) fucosidases with GlycoBuffer 1 (5 mM calcium chloride, 50 mM sodium acetate, pH 5.5) were purchased from New England BioLabs (Ipswich, MA).

Techniques:

Journal: Applied Microbiology and Biotechnology

Article Title: Degradation of the low-calorie sugar substitute 5-ketofructose by different bacteria

doi: 10.1007/s00253-021-11168-3

Figure Lengend Snippet: Strains, plasmids, and primers

Article Snippet: Escherichia coli NEB® 5-alpha (referred to as E. coli ) , F−, ø80d lacZ ΔM15, Δ( lacZYA - argF ) U169, deoR , recA 1, endA 1, hsdR 17 (rk−, mk+), phoA , supE 44, λ−, thi -1, gyr A96, rel A1 , New England Biolabs (Ipswich, USA).

Techniques: Plasmid Preparation, Strep-tag, Sequencing