fucosidase  (New England Biolabs)


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    New England Biolabs fucosidase
    Slopes of the dose response curves established using the dLISA approach correlated with TIMP-1 fucosylation. (A) Dose–response curves of serum samples spiked with differentially fucosylated recombinant TIMP-1. Recombinant TIMP-1 was treated by α1, 2 <t>fucosidase</t> at different length of time (0, 15, 30, and 45 minutes) before they were spiked into with four serum aliquots. These aliquots came from the same pool of sera with endogenous TIMP-1 immuno-depleted (confirmed by the TIMP-1 immunoassay). After the recombinant protein spiked in, these serum samples underwent the dLISA approach. (B) A graph presentation of linear regression slopes of the dose–response curves versus the length of fucosidase treatment (minutes).
    Fucosidase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fucosidase/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fucosidase - by Bioz Stars, 2023-02
    94/100 stars

    Images

    1) Product Images from "Analysis of serum protein glycosylation by a differential lectin immunosorbant assay (dLISA)"

    Article Title: Analysis of serum protein glycosylation by a differential lectin immunosorbant assay (dLISA)

    Journal: Clinical proteomics

    doi: 10.1186/1559-0275-10-12

    Slopes of the dose response curves established using the dLISA approach correlated with TIMP-1 fucosylation. (A) Dose–response curves of serum samples spiked with differentially fucosylated recombinant TIMP-1. Recombinant TIMP-1 was treated by α1, 2 fucosidase at different length of time (0, 15, 30, and 45 minutes) before they were spiked into with four serum aliquots. These aliquots came from the same pool of sera with endogenous TIMP-1 immuno-depleted (confirmed by the TIMP-1 immunoassay). After the recombinant protein spiked in, these serum samples underwent the dLISA approach. (B) A graph presentation of linear regression slopes of the dose–response curves versus the length of fucosidase treatment (minutes).
    Figure Legend Snippet: Slopes of the dose response curves established using the dLISA approach correlated with TIMP-1 fucosylation. (A) Dose–response curves of serum samples spiked with differentially fucosylated recombinant TIMP-1. Recombinant TIMP-1 was treated by α1, 2 fucosidase at different length of time (0, 15, 30, and 45 minutes) before they were spiked into with four serum aliquots. These aliquots came from the same pool of sera with endogenous TIMP-1 immuno-depleted (confirmed by the TIMP-1 immunoassay). After the recombinant protein spiked in, these serum samples underwent the dLISA approach. (B) A graph presentation of linear regression slopes of the dose–response curves versus the length of fucosidase treatment (minutes).

    Techniques Used: Recombinant

    fucosidase  (New England Biolabs)


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  • 94

    Structured Review

    New England Biolabs fucosidase
    Slopes of the dose response curves established using the dLISA approach correlated with TIMP-1 fucosylation. (A) Dose–response curves of serum samples spiked with differentially fucosylated recombinant TIMP-1. Recombinant TIMP-1 was treated by α1, 2 <t>fucosidase</t> at different length of time (0, 15, 30, and 45 minutes) before they were spiked into with four serum aliquots. These aliquots came from the same pool of sera with endogenous TIMP-1 immuno-depleted (confirmed by the TIMP-1 immunoassay). After the recombinant protein spiked in, these serum samples underwent the dLISA approach. (B) A graph presentation of linear regression slopes of the dose–response curves versus the length of fucosidase treatment (minutes).
    Fucosidase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fucosidase/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fucosidase - by Bioz Stars, 2023-02
    94/100 stars

    Images

    1) Product Images from "Analysis of serum protein glycosylation by a differential lectin immunosorbant assay (dLISA)"

    Article Title: Analysis of serum protein glycosylation by a differential lectin immunosorbant assay (dLISA)

    Journal: Clinical proteomics

    doi: 10.1186/1559-0275-10-12

    Slopes of the dose response curves established using the dLISA approach correlated with TIMP-1 fucosylation. (A) Dose–response curves of serum samples spiked with differentially fucosylated recombinant TIMP-1. Recombinant TIMP-1 was treated by α1, 2 fucosidase at different length of time (0, 15, 30, and 45 minutes) before they were spiked into with four serum aliquots. These aliquots came from the same pool of sera with endogenous TIMP-1 immuno-depleted (confirmed by the TIMP-1 immunoassay). After the recombinant protein spiked in, these serum samples underwent the dLISA approach. (B) A graph presentation of linear regression slopes of the dose–response curves versus the length of fucosidase treatment (minutes).
    Figure Legend Snippet: Slopes of the dose response curves established using the dLISA approach correlated with TIMP-1 fucosylation. (A) Dose–response curves of serum samples spiked with differentially fucosylated recombinant TIMP-1. Recombinant TIMP-1 was treated by α1, 2 fucosidase at different length of time (0, 15, 30, and 45 minutes) before they were spiked into with four serum aliquots. These aliquots came from the same pool of sera with endogenous TIMP-1 immuno-depleted (confirmed by the TIMP-1 immunoassay). After the recombinant protein spiked in, these serum samples underwent the dLISA approach. (B) A graph presentation of linear regression slopes of the dose–response curves versus the length of fucosidase treatment (minutes).

    Techniques Used: Recombinant

    α1 2 fucosidase  (New England Biolabs)


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  • 94

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    New England Biolabs α1 2 fucosidase
    α1 2 Fucosidase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/α1 2 fucosidase/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    α1 2 fucosidase - by Bioz Stars, 2023-02
    94/100 stars

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    α1 2 p0724  (New England Biolabs)


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    New England Biolabs α1 2 p0724
    XIC chromatogram of six glycoforms of the SWPAVGNCSSALR glycopeptide of hemopexin extracted from the GP-SWATH DIA data of a MARS14 depleted serum sample treated with α2-3,6,8,9 neuraminidase, <t>α1-2</t> and α1-3,4 fucosidases.
    α1 2 P0724, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/α1 2 p0724/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
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    α1 2 p0724 - by Bioz Stars, 2023-02
    94/100 stars

    Images

    1) Product Images from "Analysis of site and structure specific core fucosylation in liver cirrhosis using exoglycosidase-assisted data-independent LC-MS/MS"

    Article Title: Analysis of site and structure specific core fucosylation in liver cirrhosis using exoglycosidase-assisted data-independent LC-MS/MS

    Journal: Scientific Reports

    doi: 10.1038/s41598-021-02838-3

    XIC chromatogram of six glycoforms of the SWPAVGNCSSALR glycopeptide of hemopexin extracted from the GP-SWATH DIA data of a MARS14 depleted serum sample treated with α2-3,6,8,9 neuraminidase, α1-2 and α1-3,4 fucosidases.
    Figure Legend Snippet: XIC chromatogram of six glycoforms of the SWPAVGNCSSALR glycopeptide of hemopexin extracted from the GP-SWATH DIA data of a MARS14 depleted serum sample treated with α2-3,6,8,9 neuraminidase, α1-2 and α1-3,4 fucosidases.

    Techniques Used:

    a1 promoter  (New England Biolabs)


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    New England Biolabs a1 promoter
    A1 Promoter, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/a1 promoter/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
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    α1 2 fucosidase p0724s  (New England Biolabs)


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  • 94

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    New England Biolabs α1 2 fucosidase p0724s
    α1 2 Fucosidase P0724s, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/α1 2 fucosidase p0724s/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
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    rel a1  (New England Biolabs)


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    New England Biolabs rel a1
    Strains, plasmids, and primers
    Rel A1, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rel a1/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
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    rel a1 - by Bioz Stars, 2023-02
    94/100 stars

    Images

    1) Product Images from "Degradation of the low-calorie sugar substitute 5-ketofructose by different bacteria"

    Article Title: Degradation of the low-calorie sugar substitute 5-ketofructose by different bacteria

    Journal: Applied Microbiology and Biotechnology

    doi: 10.1007/s00253-021-11168-3

    Strains, plasmids, and primers
    Figure Legend Snippet: Strains, plasmids, and primers

    Techniques Used: Plasmid Preparation, Strep-tag, Sequencing

    α1 2 p0724  (New England Biolabs)


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  • 94

    Structured Review

    New England Biolabs α1 2 p0724
    XIC chromatogram of six glycoforms of the SWPAVGNCSSALR glycopeptide of hemopexin extracted from the GP-SWATH DIA data of a MARS14 depleted serum sample treated with α2-3,6,8,9 neuraminidase, <t>α1-2</t> and α1-3,4 fucosidases.
    α1 2 P0724, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/α1 2 p0724/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    α1 2 p0724 - by Bioz Stars, 2023-02
    94/100 stars

    Images

    1) Product Images from "Analysis of site and structure specific core fucosylation in liver disease progression using exoglycosidase-assisted data-independent LC-MS/MS"

    Article Title: Analysis of site and structure specific core fucosylation in liver disease progression using exoglycosidase-assisted data-independent LC-MS/MS

    Journal: bioRxiv

    doi: 10.1101/2020.07.29.227488

    XIC chromatogram of six glycoforms of the SWPAVGNCSSALR glycopeptide of hemopexin extracted from the GP-SWATH DIA data of a MARS14 depleted serum sample treated with α2-3,6,8,9 neuraminidase, α1-2 and α1-3,4 fucosidases.
    Figure Legend Snippet: XIC chromatogram of six glycoforms of the SWPAVGNCSSALR glycopeptide of hemopexin extracted from the GP-SWATH DIA data of a MARS14 depleted serum sample treated with α2-3,6,8,9 neuraminidase, α1-2 and α1-3,4 fucosidases.

    Techniques Used:

    α1 2 fucosidase 159  (New England Biolabs)


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    New England Biolabs α1 2 fucosidase 159
    α1 2 Fucosidase 159, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/α1 2 fucosidase 159/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
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    α1 2 fucosidase 159 - by Bioz Stars, 2023-02
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    α1 2 fucosidase  (New England Biolabs)


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  • 94

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    New England Biolabs α1 2 fucosidase
    α1 2 Fucosidase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/α1 2 fucosidase/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    α1 2 fucosidase - by Bioz Stars, 2023-02
    94/100 stars

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    a1 2 fucosidase  (New England Biolabs)


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    New England Biolabs a1 2 fucosidase
    A1 2 Fucosidase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/a1 2 fucosidase/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
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    a1 2 fucosidase - by Bioz Stars, 2023-02
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    New England Biolabs fucosidase
    Slopes of the dose response curves established using the dLISA approach correlated with TIMP-1 fucosylation. (A) Dose–response curves of serum samples spiked with differentially fucosylated recombinant TIMP-1. Recombinant TIMP-1 was treated by α1, 2 <t>fucosidase</t> at different length of time (0, 15, 30, and 45 minutes) before they were spiked into with four serum aliquots. These aliquots came from the same pool of sera with endogenous TIMP-1 immuno-depleted (confirmed by the TIMP-1 immunoassay). After the recombinant protein spiked in, these serum samples underwent the dLISA approach. (B) A graph presentation of linear regression slopes of the dose–response curves versus the length of fucosidase treatment (minutes).
    Fucosidase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fucosidase/product/New England Biolabs
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    94
    New England Biolabs α1 2 fucosidase
    Slopes of the dose response curves established using the dLISA approach correlated with TIMP-1 fucosylation. (A) Dose–response curves of serum samples spiked with differentially fucosylated recombinant TIMP-1. Recombinant TIMP-1 was treated by α1, 2 <t>fucosidase</t> at different length of time (0, 15, 30, and 45 minutes) before they were spiked into with four serum aliquots. These aliquots came from the same pool of sera with endogenous TIMP-1 immuno-depleted (confirmed by the TIMP-1 immunoassay). After the recombinant protein spiked in, these serum samples underwent the dLISA approach. (B) A graph presentation of linear regression slopes of the dose–response curves versus the length of fucosidase treatment (minutes).
    α1 2 Fucosidase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    New England Biolabs α1 2 p0724
    XIC chromatogram of six glycoforms of the SWPAVGNCSSALR glycopeptide of hemopexin extracted from the GP-SWATH DIA data of a MARS14 depleted serum sample treated with α2-3,6,8,9 neuraminidase, <t>α1-2</t> and α1-3,4 fucosidases.
    α1 2 P0724, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs a1 promoter
    XIC chromatogram of six glycoforms of the SWPAVGNCSSALR glycopeptide of hemopexin extracted from the GP-SWATH DIA data of a MARS14 depleted serum sample treated with α2-3,6,8,9 neuraminidase, <t>α1-2</t> and α1-3,4 fucosidases.
    A1 Promoter, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs α1 2 fucosidase p0724s
    XIC chromatogram of six glycoforms of the SWPAVGNCSSALR glycopeptide of hemopexin extracted from the GP-SWATH DIA data of a MARS14 depleted serum sample treated with α2-3,6,8,9 neuraminidase, <t>α1-2</t> and α1-3,4 fucosidases.
    α1 2 Fucosidase P0724s, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs rel a1
    Strains, plasmids, and primers
    Rel A1, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs α1 2 fucosidase 159
    Strains, plasmids, and primers
    α1 2 Fucosidase 159, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs a1 2 fucosidase
    Strains, plasmids, and primers
    A1 2 Fucosidase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Slopes of the dose response curves established using the dLISA approach correlated with TIMP-1 fucosylation. (A) Dose–response curves of serum samples spiked with differentially fucosylated recombinant TIMP-1. Recombinant TIMP-1 was treated by α1, 2 fucosidase at different length of time (0, 15, 30, and 45 minutes) before they were spiked into with four serum aliquots. These aliquots came from the same pool of sera with endogenous TIMP-1 immuno-depleted (confirmed by the TIMP-1 immunoassay). After the recombinant protein spiked in, these serum samples underwent the dLISA approach. (B) A graph presentation of linear regression slopes of the dose–response curves versus the length of fucosidase treatment (minutes).

    Journal: Clinical proteomics

    Article Title: Analysis of serum protein glycosylation by a differential lectin immunosorbant assay (dLISA)

    doi: 10.1186/1559-0275-10-12

    Figure Lengend Snippet: Slopes of the dose response curves established using the dLISA approach correlated with TIMP-1 fucosylation. (A) Dose–response curves of serum samples spiked with differentially fucosylated recombinant TIMP-1. Recombinant TIMP-1 was treated by α1, 2 fucosidase at different length of time (0, 15, 30, and 45 minutes) before they were spiked into with four serum aliquots. These aliquots came from the same pool of sera with endogenous TIMP-1 immuno-depleted (confirmed by the TIMP-1 immunoassay). After the recombinant protein spiked in, these serum samples underwent the dLISA approach. (B) A graph presentation of linear regression slopes of the dose–response curves versus the length of fucosidase treatment (minutes).

    Article Snippet: Four microliter of the recombinant TIMP-1 (10 μg/mL prepared in PBS + 1% BSA buffer) was mixed with 4 μL of α1, 2 fucosidase (Catalog# P0724, New England Biolabs, Ipswich, MA).

    Techniques: Recombinant

    XIC chromatogram of six glycoforms of the SWPAVGNCSSALR glycopeptide of hemopexin extracted from the GP-SWATH DIA data of a MARS14 depleted serum sample treated with α2-3,6,8,9 neuraminidase, α1-2 and α1-3,4 fucosidases.

    Journal: Scientific Reports

    Article Title: Analysis of site and structure specific core fucosylation in liver cirrhosis using exoglycosidase-assisted data-independent LC-MS/MS

    doi: 10.1038/s41598-021-02838-3

    Figure Lengend Snippet: XIC chromatogram of six glycoforms of the SWPAVGNCSSALR glycopeptide of hemopexin extracted from the GP-SWATH DIA data of a MARS14 depleted serum sample treated with α2-3,6,8,9 neuraminidase, α1-2 and α1-3,4 fucosidases.

    Article Snippet: Trypsin Gold (V5280) was obtained from Promega (Madison, WI). α2-3,6,8,9 neuraminidase (P0722), α1-2 (P0724) and α1-3,4 (P0769) fucosidases with GlycoBuffer 1 (5 mM calcium chloride, 50 mM sodium acetate, pH 5.5) were purchased from New England BioLabs (Ipswich, MA).

    Techniques:

    Strains, plasmids, and primers

    Journal: Applied Microbiology and Biotechnology

    Article Title: Degradation of the low-calorie sugar substitute 5-ketofructose by different bacteria

    doi: 10.1007/s00253-021-11168-3

    Figure Lengend Snippet: Strains, plasmids, and primers

    Article Snippet: Escherichia coli NEB® 5-alpha (referred to as E. coli ) , F−, ø80d lacZ ΔM15, Δ( lacZYA - argF ) U169, deoR , recA 1, endA 1, hsdR 17 (rk−, mk+), phoA , supE 44, λ−, thi -1, gyr A96, rel A1 , New England Biolabs (Ipswich, USA).

    Techniques: Plasmid Preparation, Strep-tag, Sequencing