p0720  (New England Biolabs)


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    Structured Review

    New England Biolabs p0720
    P0720, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p0720/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    p0720 - by Bioz Stars, 2022-12
    95/100 stars

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    New England Biolabs alpha 2 3 6 8 neuraminidase
    Schematic representation of transglycosylation of palivizumab to generate homogeneous glycoforms. (A) Deglycosylation of commercially-available palivizumab using EndoS D233Q generates the truncated GlcNAcFuc disaccharide (blue square = GlcNAc; red triangle = fucose) remaining on palivizumab. Defucosylation is achieved using fucosidase GH29, and subsequent glycan ligation of purified glycan oxazoline is achieved using EndoS 233Q. (B) Enzymatic reaction and purification of truncated glycans (mono-galactosylated at the α3 antenna (G1 (3)T) and digalactosylated (G2T)) using EndoS and <t>α2-3,6,8</t> neuraminidase to cleave truncated glycans between the reducing end chitobiose core, followed by purification by porous graphitic carbon (PGC)-HPLC. (C) Synthesis of truncated agalactosylated (G0T) and monogalactosylated at the α6 antenna (G1 (6)T) glycans using LacZ β1-4 galactosidase (* indicates reaction byproduct peaks), followed by purification by PGC-HPLC. HPAEC-PAD trace of overlaid spectra of purified glycans are shown on the right in each of (B, C) .
    Alpha 2 3 6 8 Neuraminidase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 105 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alpha 2 3 6 8 neuraminidase/product/New England Biolabs
    Average 94 stars, based on 105 article reviews
    Price from $9.99 to $1999.99
    alpha 2 3 6 8 neuraminidase - by Bioz Stars, 2022-12
    94/100 stars
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    Schematic representation of transglycosylation of palivizumab to generate homogeneous glycoforms. (A) Deglycosylation of commercially-available palivizumab using EndoS D233Q generates the truncated GlcNAcFuc disaccharide (blue square = GlcNAc; red triangle = fucose) remaining on palivizumab. Defucosylation is achieved using fucosidase GH29, and subsequent glycan ligation of purified glycan oxazoline is achieved using EndoS 233Q. (B) Enzymatic reaction and purification of truncated glycans (mono-galactosylated at the α3 antenna (G1 (3)T) and digalactosylated (G2T)) using EndoS and α2-3,6,8 neuraminidase to cleave truncated glycans between the reducing end chitobiose core, followed by purification by porous graphitic carbon (PGC)-HPLC. (C) Synthesis of truncated agalactosylated (G0T) and monogalactosylated at the α6 antenna (G1 (6)T) glycans using LacZ β1-4 galactosidase (* indicates reaction byproduct peaks), followed by purification by PGC-HPLC. HPAEC-PAD trace of overlaid spectra of purified glycans are shown on the right in each of (B, C) .

    Journal: Frontiers in Immunology

    Article Title: Specific location of galactosylation in an afucosylated antiviral monoclonal antibody affects its FcγRIIIA binding affinity

    doi: 10.3389/fimmu.2022.972168

    Figure Lengend Snippet: Schematic representation of transglycosylation of palivizumab to generate homogeneous glycoforms. (A) Deglycosylation of commercially-available palivizumab using EndoS D233Q generates the truncated GlcNAcFuc disaccharide (blue square = GlcNAc; red triangle = fucose) remaining on palivizumab. Defucosylation is achieved using fucosidase GH29, and subsequent glycan ligation of purified glycan oxazoline is achieved using EndoS 233Q. (B) Enzymatic reaction and purification of truncated glycans (mono-galactosylated at the α3 antenna (G1 (3)T) and digalactosylated (G2T)) using EndoS and α2-3,6,8 neuraminidase to cleave truncated glycans between the reducing end chitobiose core, followed by purification by porous graphitic carbon (PGC)-HPLC. (C) Synthesis of truncated agalactosylated (G0T) and monogalactosylated at the α6 antenna (G1 (6)T) glycans using LacZ β1-4 galactosidase (* indicates reaction byproduct peaks), followed by purification by PGC-HPLC. HPAEC-PAD trace of overlaid spectra of purified glycans are shown on the right in each of (B, C) .

    Article Snippet: 5 µL α2-3,6,8 neuraminidase (NEB, Cat #P0720) was then added to desialylate glycans.

    Techniques: Ligation, Purification, Pyrolysis Gas Chromatography, High Performance Liquid Chromatography