p0705s  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    New England Biolabs p0705s
    P0705s, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p0705s/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    p0705s - by Bioz Stars, 2022-12
    94/100 stars

    Images

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    New England Biolabs pngase f glycerol free
    Study Design and Proteomic Pipeline for ECM Analysis of Lung Squamous Cell Carcinoma. ‘Tumor’ and ‘Matched Normal’ fresh tissue specimens were collected from 10 patients with lung squamous cell carcinoma by the Ferri team at McGill University Health Centre (QC, Canada) or through the Cooperative Human Tissue Network Western Division (TN, USA). Fresh samples in UW Cold Storage Solution were sent to the Tlsty team at UCSF (CA, USA) for enrichment for insoluble extracellular matrix (ECM) proteins. ECM-enriched samples were further processed by the Schilling team at the Buck Institute (Novato, CA); proteins were solubilized, in-gel digested with Lys-C and trypsin, and extracted proteolytic peptides were de-glycosylated with PNGase F. All resulting samples were analyzed in duplicate on a nanoLC-TripleTOF 6600 system (QqTOF) operated in data-independent acquisition (DIA) mode, and data were processed with Spectronaut (Biognosys). Finally, candidates were validated on independent cohorts by immunofluorescence-based immunohistochemistry by the Tlsty team.
    Pngase F Glycerol Free, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pngase f glycerol free/product/New England Biolabs
    Average 94 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    pngase f glycerol free - by Bioz Stars, 2022-12
    94/100 stars
      Buy from Supplier

    Image Search Results


    Study Design and Proteomic Pipeline for ECM Analysis of Lung Squamous Cell Carcinoma. ‘Tumor’ and ‘Matched Normal’ fresh tissue specimens were collected from 10 patients with lung squamous cell carcinoma by the Ferri team at McGill University Health Centre (QC, Canada) or through the Cooperative Human Tissue Network Western Division (TN, USA). Fresh samples in UW Cold Storage Solution were sent to the Tlsty team at UCSF (CA, USA) for enrichment for insoluble extracellular matrix (ECM) proteins. ECM-enriched samples were further processed by the Schilling team at the Buck Institute (Novato, CA); proteins were solubilized, in-gel digested with Lys-C and trypsin, and extracted proteolytic peptides were de-glycosylated with PNGase F. All resulting samples were analyzed in duplicate on a nanoLC-TripleTOF 6600 system (QqTOF) operated in data-independent acquisition (DIA) mode, and data were processed with Spectronaut (Biognosys). Finally, candidates were validated on independent cohorts by immunofluorescence-based immunohistochemistry by the Tlsty team.

    Journal: bioRxiv

    Article Title: Data-Independent Acquisition and Quantification of Extracellular Matrix from Human Lung in Chronic Inflammation-Associated Carcinomas

    doi: 10.1101/2022.08.05.503012

    Figure Lengend Snippet: Study Design and Proteomic Pipeline for ECM Analysis of Lung Squamous Cell Carcinoma. ‘Tumor’ and ‘Matched Normal’ fresh tissue specimens were collected from 10 patients with lung squamous cell carcinoma by the Ferri team at McGill University Health Centre (QC, Canada) or through the Cooperative Human Tissue Network Western Division (TN, USA). Fresh samples in UW Cold Storage Solution were sent to the Tlsty team at UCSF (CA, USA) for enrichment for insoluble extracellular matrix (ECM) proteins. ECM-enriched samples were further processed by the Schilling team at the Buck Institute (Novato, CA); proteins were solubilized, in-gel digested with Lys-C and trypsin, and extracted proteolytic peptides were de-glycosylated with PNGase F. All resulting samples were analyzed in duplicate on a nanoLC-TripleTOF 6600 system (QqTOF) operated in data-independent acquisition (DIA) mode, and data were processed with Spectronaut (Biognosys). Finally, candidates were validated on independent cohorts by immunofluorescence-based immunohistochemistry by the Tlsty team.

    Article Snippet: Glycerol-free PNGase F was purchased from New England BioLabs (Ipswich, MA).

    Techniques: Western Blot, Immunofluorescence, Immunohistochemistry

    Wild-type threonine 618 is modified by glycosylation. A) Workflow for digesting a soluble form of the wild-type CSF3R 5FnIII domain with sialidase, the protease OpeRATOR, and PNGase F. B) Electron transfer dissociation (ETD) mass spectrum for the glycopeptide including the Gal-GalNAc (365 Da) glycan at T618. C) Observed glycopeptides throughout the CSF3R 5FnIII sequence, including the GalNAc (203 Da) glycan at T618 and Gal-GalNAc glycan at T612.

    Journal: bioRxiv

    Article Title: Mutational screens highlight glycosylation as a modulator of CSF3R activity

    doi: 10.1101/2022.08.01.502088

    Figure Lengend Snippet: Wild-type threonine 618 is modified by glycosylation. A) Workflow for digesting a soluble form of the wild-type CSF3R 5FnIII domain with sialidase, the protease OpeRATOR, and PNGase F. B) Electron transfer dissociation (ETD) mass spectrum for the glycopeptide including the Gal-GalNAc (365 Da) glycan at T618. C) Observed glycopeptides throughout the CSF3R 5FnIII sequence, including the GalNAc (203 Da) glycan at T618 and Gal-GalNAc glycan at T612.

    Article Snippet: After 18 hours, the sample was diluted to 50 μL with ammonium bicarbonate buffer and incubated at 37 °C with 2 μL of PNGase F (New England Biolabs P0705S) diluted 1:100 in ammonium bicarbonate for an additional 7 hours.

    Techniques: Modification, Sequencing