onetaq quick load dna polymerase  (New England Biolabs)


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    New England Biolabs onetaq quick load dna polymerase
    Onetaq Quick Load Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    onetaq 2x master mix  (New England Biolabs)


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    New England Biolabs onetaq 2x master mix
    Onetaq 2x Master Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    onetaq hot start 2x master mix  (New England Biolabs)


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    New England Biolabs onetaq hot start 2x master mix
    Onetaq Hot Start 2x Master Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    onetaq dna polymerase  (New England Biolabs)


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    New England Biolabs onetaq dna polymerase
    Onetaq Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    onetaq 2ξ master mix  (New England Biolabs)


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    New England Biolabs onetaq 2ξ master mix
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    onetaq 2x master mix  (New England Biolabs)


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    New England Biolabs onetaq 2x master mix
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    onetaq dna polymerase  (New England Biolabs)


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    New England Biolabs onetaq dna polymerase
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    onetaq rt pcr kit  (New England Biolabs)


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    New England Biolabs onetaq rt pcr kit
    The def1 attenuator mutant ( def1 atten ) overexpresses mRNA and protein, exacerbating the cell toxicity of processed Def1 ( def1 1–530 ). a) Schematic of def1 mutants ( def1-atten and def1 1–530 ) along with <t>RT-PCR</t> primers (F1, R1, blue) used to detect the DEF1 mRNA readthrough product. Note: the drawing is not to scale. The R1 primer is specific to longer mRNA and not attenuated RNA. DNA positions are numbered relative to the +1 start codon of the DEF1 open reading frame. b) RT-PCR analysis of Def1 mRNA levels. 18S serves as a loading control for total RNA. Reverse Transcriptase (±RT) ensures that signal is dependent on RNA and not genomic DNA template. c) Western blot analysis of Def1 protein levels. Actin serves as a loading control for total protein. d) Spot test assay of def1 mutants on solid plate media. Liquid cultures were grown to saturation at 25°C, serially diluted, spotted onto YPAD, and grown at the temperatures indicated for 3–5 days. e, f) Growth of def1 mutants in liquid culture. Liquid yeast cultures were grown to saturation at 25°C, diluted back, and recovered to exponential phase prior to shifting to (e) 37°C or (f) 39°C for 6 h. Cell density was measured via OD 600 every hour, and doubling times were calculated from exponential lines of best fit for data between 60 and 360 min. Error bars represent SD from 3 biological replicates. Asterisks indicate statistical significance by Welch’s 2 sample t -test (* P ≤0.05, ** P ≤0.01, *** P ≤0.001, **** P ≤0.0001, ns—not significant).
    Onetaq Rt Pcr Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "RNA polymerase II transcription attenuation at the yeast DNA repair gene DEF1 is biologically significant and dependent on the Hrp1 RNA-recognition motif"

    Article Title: RNA polymerase II transcription attenuation at the yeast DNA repair gene DEF1 is biologically significant and dependent on the Hrp1 RNA-recognition motif

    Journal: G3: Genes|Genomes|Genetics

    doi: 10.1093/g3journal/jkac292

    The def1 attenuator mutant ( def1 atten ) overexpresses mRNA and protein, exacerbating the cell toxicity of processed Def1 ( def1 1–530 ). a) Schematic of def1 mutants ( def1-atten and def1 1–530 ) along with RT-PCR primers (F1, R1, blue) used to detect the DEF1 mRNA readthrough product. Note: the drawing is not to scale. The R1 primer is specific to longer mRNA and not attenuated RNA. DNA positions are numbered relative to the +1 start codon of the DEF1 open reading frame. b) RT-PCR analysis of Def1 mRNA levels. 18S serves as a loading control for total RNA. Reverse Transcriptase (±RT) ensures that signal is dependent on RNA and not genomic DNA template. c) Western blot analysis of Def1 protein levels. Actin serves as a loading control for total protein. d) Spot test assay of def1 mutants on solid plate media. Liquid cultures were grown to saturation at 25°C, serially diluted, spotted onto YPAD, and grown at the temperatures indicated for 3–5 days. e, f) Growth of def1 mutants in liquid culture. Liquid yeast cultures were grown to saturation at 25°C, diluted back, and recovered to exponential phase prior to shifting to (e) 37°C or (f) 39°C for 6 h. Cell density was measured via OD 600 every hour, and doubling times were calculated from exponential lines of best fit for data between 60 and 360 min. Error bars represent SD from 3 biological replicates. Asterisks indicate statistical significance by Welch’s 2 sample t -test (* P ≤0.05, ** P ≤0.01, *** P ≤0.001, **** P ≤0.0001, ns—not significant).
    Figure Legend Snippet: The def1 attenuator mutant ( def1 atten ) overexpresses mRNA and protein, exacerbating the cell toxicity of processed Def1 ( def1 1–530 ). a) Schematic of def1 mutants ( def1-atten and def1 1–530 ) along with RT-PCR primers (F1, R1, blue) used to detect the DEF1 mRNA readthrough product. Note: the drawing is not to scale. The R1 primer is specific to longer mRNA and not attenuated RNA. DNA positions are numbered relative to the +1 start codon of the DEF1 open reading frame. b) RT-PCR analysis of Def1 mRNA levels. 18S serves as a loading control for total RNA. Reverse Transcriptase (±RT) ensures that signal is dependent on RNA and not genomic DNA template. c) Western blot analysis of Def1 protein levels. Actin serves as a loading control for total protein. d) Spot test assay of def1 mutants on solid plate media. Liquid cultures were grown to saturation at 25°C, serially diluted, spotted onto YPAD, and grown at the temperatures indicated for 3–5 days. e, f) Growth of def1 mutants in liquid culture. Liquid yeast cultures were grown to saturation at 25°C, diluted back, and recovered to exponential phase prior to shifting to (e) 37°C or (f) 39°C for 6 h. Cell density was measured via OD 600 every hour, and doubling times were calculated from exponential lines of best fit for data between 60 and 360 min. Error bars represent SD from 3 biological replicates. Asterisks indicate statistical significance by Welch’s 2 sample t -test (* P ≤0.05, ** P ≤0.01, *** P ≤0.001, **** P ≤0.0001, ns—not significant).

    Techniques Used: Mutagenesis, Reverse Transcription Polymerase Chain Reaction, Western Blot, Spot Test

    onetaq hot start 2x master mix  (New England Biolabs)


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    New England Biolabs onetaq hot start 2x master mix
    Onetaq Hot Start 2x Master Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    onetaq dna polymerase kit  (New England Biolabs)


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    New England Biolabs onetaq dna polymerase kit
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    New England Biolabs onetaq quick load dna polymerase
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    New England Biolabs onetaq rt pcr kit
    The def1 attenuator mutant ( def1 atten ) overexpresses mRNA and protein, exacerbating the cell toxicity of processed Def1 ( def1 1–530 ). a) Schematic of def1 mutants ( def1-atten and def1 1–530 ) along with <t>RT-PCR</t> primers (F1, R1, blue) used to detect the DEF1 mRNA readthrough product. Note: the drawing is not to scale. The R1 primer is specific to longer mRNA and not attenuated RNA. DNA positions are numbered relative to the +1 start codon of the DEF1 open reading frame. b) RT-PCR analysis of Def1 mRNA levels. 18S serves as a loading control for total RNA. Reverse Transcriptase (±RT) ensures that signal is dependent on RNA and not genomic DNA template. c) Western blot analysis of Def1 protein levels. Actin serves as a loading control for total protein. d) Spot test assay of def1 mutants on solid plate media. Liquid cultures were grown to saturation at 25°C, serially diluted, spotted onto YPAD, and grown at the temperatures indicated for 3–5 days. e, f) Growth of def1 mutants in liquid culture. Liquid yeast cultures were grown to saturation at 25°C, diluted back, and recovered to exponential phase prior to shifting to (e) 37°C or (f) 39°C for 6 h. Cell density was measured via OD 600 every hour, and doubling times were calculated from exponential lines of best fit for data between 60 and 360 min. Error bars represent SD from 3 biological replicates. Asterisks indicate statistical significance by Welch’s 2 sample t -test (* P ≤0.05, ** P ≤0.01, *** P ≤0.001, **** P ≤0.0001, ns—not significant).
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    New England Biolabs onetaq dna polymerase kit
    The def1 attenuator mutant ( def1 atten ) overexpresses mRNA and protein, exacerbating the cell toxicity of processed Def1 ( def1 1–530 ). a) Schematic of def1 mutants ( def1-atten and def1 1–530 ) along with <t>RT-PCR</t> primers (F1, R1, blue) used to detect the DEF1 mRNA readthrough product. Note: the drawing is not to scale. The R1 primer is specific to longer mRNA and not attenuated RNA. DNA positions are numbered relative to the +1 start codon of the DEF1 open reading frame. b) RT-PCR analysis of Def1 mRNA levels. 18S serves as a loading control for total RNA. Reverse Transcriptase (±RT) ensures that signal is dependent on RNA and not genomic DNA template. c) Western blot analysis of Def1 protein levels. Actin serves as a loading control for total protein. d) Spot test assay of def1 mutants on solid plate media. Liquid cultures were grown to saturation at 25°C, serially diluted, spotted onto YPAD, and grown at the temperatures indicated for 3–5 days. e, f) Growth of def1 mutants in liquid culture. Liquid yeast cultures were grown to saturation at 25°C, diluted back, and recovered to exponential phase prior to shifting to (e) 37°C or (f) 39°C for 6 h. Cell density was measured via OD 600 every hour, and doubling times were calculated from exponential lines of best fit for data between 60 and 360 min. Error bars represent SD from 3 biological replicates. Asterisks indicate statistical significance by Welch’s 2 sample t -test (* P ≤0.05, ** P ≤0.01, *** P ≤0.001, **** P ≤0.0001, ns—not significant).
    Onetaq Dna Polymerase Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    The def1 attenuator mutant ( def1 atten ) overexpresses mRNA and protein, exacerbating the cell toxicity of processed Def1 ( def1 1–530 ). a) Schematic of def1 mutants ( def1-atten and def1 1–530 ) along with RT-PCR primers (F1, R1, blue) used to detect the DEF1 mRNA readthrough product. Note: the drawing is not to scale. The R1 primer is specific to longer mRNA and not attenuated RNA. DNA positions are numbered relative to the +1 start codon of the DEF1 open reading frame. b) RT-PCR analysis of Def1 mRNA levels. 18S serves as a loading control for total RNA. Reverse Transcriptase (±RT) ensures that signal is dependent on RNA and not genomic DNA template. c) Western blot analysis of Def1 protein levels. Actin serves as a loading control for total protein. d) Spot test assay of def1 mutants on solid plate media. Liquid cultures were grown to saturation at 25°C, serially diluted, spotted onto YPAD, and grown at the temperatures indicated for 3–5 days. e, f) Growth of def1 mutants in liquid culture. Liquid yeast cultures were grown to saturation at 25°C, diluted back, and recovered to exponential phase prior to shifting to (e) 37°C or (f) 39°C for 6 h. Cell density was measured via OD 600 every hour, and doubling times were calculated from exponential lines of best fit for data between 60 and 360 min. Error bars represent SD from 3 biological replicates. Asterisks indicate statistical significance by Welch’s 2 sample t -test (* P ≤0.05, ** P ≤0.01, *** P ≤0.001, **** P ≤0.0001, ns—not significant).

    Journal: G3: Genes|Genomes|Genetics

    Article Title: RNA polymerase II transcription attenuation at the yeast DNA repair gene DEF1 is biologically significant and dependent on the Hrp1 RNA-recognition motif

    doi: 10.1093/g3journal/jkac292

    Figure Lengend Snippet: The def1 attenuator mutant ( def1 atten ) overexpresses mRNA and protein, exacerbating the cell toxicity of processed Def1 ( def1 1–530 ). a) Schematic of def1 mutants ( def1-atten and def1 1–530 ) along with RT-PCR primers (F1, R1, blue) used to detect the DEF1 mRNA readthrough product. Note: the drawing is not to scale. The R1 primer is specific to longer mRNA and not attenuated RNA. DNA positions are numbered relative to the +1 start codon of the DEF1 open reading frame. b) RT-PCR analysis of Def1 mRNA levels. 18S serves as a loading control for total RNA. Reverse Transcriptase (±RT) ensures that signal is dependent on RNA and not genomic DNA template. c) Western blot analysis of Def1 protein levels. Actin serves as a loading control for total protein. d) Spot test assay of def1 mutants on solid plate media. Liquid cultures were grown to saturation at 25°C, serially diluted, spotted onto YPAD, and grown at the temperatures indicated for 3–5 days. e, f) Growth of def1 mutants in liquid culture. Liquid yeast cultures were grown to saturation at 25°C, diluted back, and recovered to exponential phase prior to shifting to (e) 37°C or (f) 39°C for 6 h. Cell density was measured via OD 600 every hour, and doubling times were calculated from exponential lines of best fit for data between 60 and 360 min. Error bars represent SD from 3 biological replicates. Asterisks indicate statistical significance by Welch’s 2 sample t -test (* P ≤0.05, ** P ≤0.01, *** P ≤0.001, **** P ≤0.0001, ns—not significant).

    Article Snippet: After the RNA was isolated, mRNA was converted to cDNA using the OneTaq RT-PCR kit (New England BioLabs).

    Techniques: Mutagenesis, Reverse Transcription Polymerase Chain Reaction, Western Blot, Spot Test