onetaq (New England Biolabs)
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New England Biolabs
onetaq
Onetaq, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/onetaq/product/New England Biolabs
Average 99 stars, based on 3 article reviews
Price from $9.99 to $1999.99
Onetaq, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/onetaq/product/New England Biolabs
Average 99 stars, based on 3 article reviews
Price from $9.99 to $1999.99
onetaq - by Bioz Stars,
2020-02
99/100 stars
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DNA Extraction:Article Title: Ubiquity and Diversity of Human-Associated Demodex Mites Article Snippet: Paragraph title: (b) DNA Extraction and PCR ... We used either Article Title: The jellyfish Cassiopea exhibits a sleep-like state Article Snippet: Jellyfish fragments, about 2 mm of tissue from the tentacles, were placed in 400 μL DNA extraction buffer (50% w/v guanidinium isothiocyanate; 50 mM Tris pH 7.6; 10 μM EDTA; 4.2% w/v sarkosyl; 2.1% v/v β-mercaptoethanol). .. COI primers: LCO1490 forward primer: 5′-ggtcaacaaatcataaagatattgg-3′ HC02198 reverse primer: 5′-taaacttcagggtgaccaaaaaatca-3′ Amplifications were performed under the following PCR conditions: 2 min at 92°C, 30 cycles of 94°C for 30 s, 55°C for 30 s and 72°C for 45 s, with a final 72°C extension for 7 min. Amplification products were then TOPO-cloned using Clone Assay:Article Title: A Distalless-responsive enhancer of the Hox gene Sex combs reduced is required for segment- and sex-specific sensory organ development in Drosophila Article Snippet: Generation of the CRISPR line To generate the pGX-2attp_WN_Scr_A-B replacement donor [ ], 5.299kb 5'-homology arm and 5.127kb 3'-homology arm were amplified and initially cloned in the pCR4-TOPO Vector (Invitrogen) and pCRII Vector (Invitrogen) respectively. .. The 3'-homology arm was amplified ( Centrifugation:Article Title: The jellyfish Cassiopea exhibits a sleep-like state Article Snippet: The DNA was precipitated by centrifugation at 16,000 g for 15 min and the DNA pellet washed in 70% ethanol and resuspended and stored in water. .. COI primers: LCO1490 forward primer: 5′-ggtcaacaaatcataaagatattgg-3′ HC02198 reverse primer: 5′-taaacttcagggtgaccaaaaaatca-3′ Amplifications were performed under the following PCR conditions: 2 min at 92°C, 30 cycles of 94°C for 30 s, 55°C for 30 s and 72°C for 45 s, with a final 72°C extension for 7 min. Amplification products were then TOPO-cloned using Amplification:Article Title: A Distalless-responsive enhancer of the Hox gene Sex combs reduced is required for segment- and sex-specific sensory organ development in Drosophila Article Snippet: .. The 3'-homology arm was amplified ( Article Title: Compounds that select against the tetracycline resistance efflux pump Article Snippet: .. Detection of tetA , tetR, and marR by PCR The tetA , tetR, and marR genes were amplified with the primers ( ) in 25 μL reactions using 0.2 μL Article Title: Loss of WSTF results in spontaneous fluctuations of heterochromatin formation and resolution, combined with substantial changes to gene expression Article Snippet: Standard RT-PCR Analysis Standard RT-PCR was performed using Article Title: A Distalless-responsive enhancer of the Hox gene Sex combs reduced is required for segment- and sex-specific sensory organ development in Drosophila Article Snippet: .. The 5'-homology arm was amplified ( Article Title: The jellyfish Cassiopea exhibits a sleep-like state Article Snippet: .. COI primers: LCO1490 forward primer: 5′-ggtcaacaaatcataaagatattgg-3′ HC02198 reverse primer: 5′-taaacttcagggtgaccaaaaaatca-3′ Amplifications were performed under the following PCR conditions: 2 min at 92°C, 30 cycles of 94°C for 30 s, 55°C for 30 s and 72°C for 45 s, with a final 72°C extension for 7 min. Amplification products were then TOPO-cloned using Agarose Gel Electrophoresis:Article Title: Compounds that select against the tetracycline resistance efflux pump Article Snippet: Detection of tetA , tetR, and marR by PCR The tetA , tetR, and marR genes were amplified with the primers ( ) in 25 μL reactions using 0.2 μL Nucleic Acid Electrophoresis:Article Title: Compounds that select against the tetracycline resistance efflux pump Article Snippet: Detection of tetA , tetR, and marR by PCR The tetA , tetR, and marR genes were amplified with the primers ( ) in 25 μL reactions using 0.2 μL Sequencing:Article Title: A growth‐ and bioluminescence‐based bioreporter for the in vivo detection of novel biocatalysts Article Snippet: PCRs were performed with Article Title: Ubiquity and Diversity of Human-Associated Demodex Mites Article Snippet: .. We used either Article Title: Cell type-dependent axonal localization of translational regulators and mRNA in mouse peripheral olfactory neurons Article Snippet: PCR from each cDNA sample was performed as indicated in Results using Article Title: The jellyfish Cassiopea exhibits a sleep-like state Article Snippet: To assess the diversity of Cassiopea spp. within our population we genotyped several animals by amplification and sequencing of the Mitochondrial cytochrome c oxidase I (COI). .. COI primers: LCO1490 forward primer: 5′-ggtcaacaaatcataaagatattgg-3′ HC02198 reverse primer: 5′-taaacttcagggtgaccaaaaaatca-3′ Amplifications were performed under the following PCR conditions: 2 min at 92°C, 30 cycles of 94°C for 30 s, 55°C for 30 s and 72°C for 45 s, with a final 72°C extension for 7 min. Amplification products were then TOPO-cloned using Generated:Article Title: A Distalless-responsive enhancer of the Hox gene Sex combs reduced is required for segment- and sex-specific sensory organ development in Drosophila Article Snippet: The 3'-homology arm was amplified ( Article Title: The jellyfish Cassiopea exhibits a sleep-like state Article Snippet: COI primers: LCO1490 forward primer: 5′-ggtcaacaaatcataaagatattgg-3′ HC02198 reverse primer: 5′-taaacttcagggtgaccaaaaaatca-3′ Amplifications were performed under the following PCR conditions: 2 min at 92°C, 30 cycles of 94°C for 30 s, 55°C for 30 s and 72°C for 45 s, with a final 72°C extension for 7 min. Amplification products were then TOPO-cloned using Construct:Article Title: A Distalless-responsive enhancer of the Hox gene Sex combs reduced is required for segment- and sex-specific sensory organ development in Drosophila Article Snippet: The 3'-homology arm was amplified ( Mouse Assay:Article Title: Cell type-dependent axonal localization of translational regulators and mRNA in mouse peripheral olfactory neurons Article Snippet: Tissue was dissected from 75-day-old mice, and RNA was prepared using Trizol (Thermo Fisher). .. PCR from each cDNA sample was performed as indicated in Results using Reverse Transcription Polymerase Chain Reaction:Article Title: Loss of WSTF results in spontaneous fluctuations of heterochromatin formation and resolution, combined with substantial changes to gene expression Article Snippet: .. Standard RT-PCR Analysis Standard RT-PCR was performed using Article Title: Ca2+-Induced Two-Component System CvsSR Regulates the Type III Secretion System and the Extracytoplasmic Function Sigma Factor AlgU in Pseudomonas syringae pv. tomato DC3000 Article Snippet: .. RT-PCR was performed on P. syringae pv. tomato DC3000 genomic DNA, cDNA, and an RNA control without reverse transcriptase on a Bio-Rad T100 thermocycler using Marker:Article Title: A Distalless-responsive enhancer of the Hox gene Sex combs reduced is required for segment- and sex-specific sensory organ development in Drosophila Article Snippet: The 3'-homology arm was amplified ( Incubation:Article Title: Ubiquity and Diversity of Human-Associated Demodex Mites Article Snippet: The samples were incubated overnight at 56°C with 180 µl of ATL buffer and 20 µl proteinase K. The final elution step was performed with 150 µl of elution buffer warmed to 56°C. .. We used either Activity Assay:Article Title: A growth‐ and bioluminescence‐based bioreporter for the in vivo detection of novel biocatalysts Article Snippet: Paragraph title: Enrichment for cells with l ‐arabinose isomerase activity ... PCRs were performed with CRISPR:Article Title: A Distalless-responsive enhancer of the Hox gene Sex combs reduced is required for segment- and sex-specific sensory organ development in Drosophila Article Snippet: Paragraph title: Generation of the CRISPR line ... The 3'-homology arm was amplified ( Polymerase Chain Reaction:Article Title: Ubiquity and Diversity of Human-Associated Demodex Mites Article Snippet: .. We used either Article Title: Compounds that select against the tetracycline resistance efflux pump Article Snippet: .. Detection of tetA , tetR, and marR by PCR The tetA , tetR, and marR genes were amplified with the primers ( ) in 25 μL reactions using 0.2 μL Article Title: Cell type-dependent axonal localization of translational regulators and mRNA in mouse peripheral olfactory neurons Article Snippet: .. PCR from each cDNA sample was performed as indicated in Results using Article Title: Ca2+-Induced Two-Component System CvsSR Regulates the Type III Secretion System and the Extracytoplasmic Function Sigma Factor AlgU in Pseudomonas syringae pv. tomato DC3000 Article Snippet: RT-PCR was performed on P. syringae pv. tomato DC3000 genomic DNA, cDNA, and an RNA control without reverse transcriptase on a Bio-Rad T100 thermocycler using Article Title: The jellyfish Cassiopea exhibits a sleep-like state Article Snippet: .. COI primers: LCO1490 forward primer: 5′-ggtcaacaaatcataaagatattgg-3′ HC02198 reverse primer: 5′-taaacttcagggtgaccaaaaaatca-3′ Amplifications were performed under the following PCR conditions: 2 min at 92°C, 30 cycles of 94°C for 30 s, 55°C for 30 s and 72°C for 45 s, with a final 72°C extension for 7 min. Amplification products were then TOPO-cloned using Transgenic Assay:Article Title: A Distalless-responsive enhancer of the Hox gene Sex combs reduced is required for segment- and sex-specific sensory organ development in Drosophila Article Snippet: The 3'-homology arm was amplified ( Transformation Assay:Article Title: A Distalless-responsive enhancer of the Hox gene Sex combs reduced is required for segment- and sex-specific sensory organ development in Drosophila Article Snippet: The 3'-homology arm was amplified ( Plasmid Preparation:Article Title: A Distalless-responsive enhancer of the Hox gene Sex combs reduced is required for segment- and sex-specific sensory organ development in Drosophila Article Snippet: Generation of the CRISPR line To generate the pGX-2attp_WN_Scr_A-B replacement donor [ ], 5.299kb 5'-homology arm and 5.127kb 3'-homology arm were amplified and initially cloned in the pCR4-TOPO Vector (Invitrogen) and pCRII Vector (Invitrogen) respectively. .. The 3'-homology arm was amplified ( Software:Article Title: The jellyfish Cassiopea exhibits a sleep-like state Article Snippet: COI primers: LCO1490 forward primer: 5′-ggtcaacaaatcataaagatattgg-3′ HC02198 reverse primer: 5′-taaacttcagggtgaccaaaaaatca-3′ Amplifications were performed under the following PCR conditions: 2 min at 92°C, 30 cycles of 94°C for 30 s, 55°C for 30 s and 72°C for 45 s, with a final 72°C extension for 7 min. Amplification products were then TOPO-cloned using |
