onetaq  (New England Biolabs)


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  • 96
    Name:
    OneTaq 2X Master Mix with Standard Buffer
    Description:
    OneTaq 2X Master Mix with Standard Buffer 500 rxns
    Catalog Number:
    M0482L
    Price:
    188
    Category:
    Thermostable DNA Polymerases
    Size:
    500 rxns
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    Structured Review

    New England Biolabs onetaq
    OneTaq 2X Master Mix with Standard Buffer
    OneTaq 2X Master Mix with Standard Buffer 500 rxns
    https://www.bioz.com/result/onetaq/product/New England Biolabs
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    onetaq - by Bioz Stars, 2021-09
    96/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Genetic Defects in DNAH2 Underlie Male Infertility With Multiple Morphological Abnormalities of the Sperm Flagella in Humans and Mice
    Article Snippet: .. The cDNAs were applied for PCR using OneTaq® 2X Master Mix (NEB) or quantitative PCR using iTaq Universal SYBR Green Supermix (Bio-Rad). ..

    Article Title: Inhibition of arginase modulates T-cell response in the tumor microenvironment of lung carcinoma
    Article Snippet: .. PCR reaction was set up using OneTaq® 2× Master Mix with Standard Buffer (New England Biolabs) and appropriate primers listed in the online supplementary table 2. ..

    Article Title: C2cd6-encoded CatSperτ Targets Sperm Calcium Channel to Ca2+ Signaling Domains in the Flagellar Membrane
    Article Snippet: .. 500 ng of the extracted RNA was used for cDNA synthesis using iScriptTM cDNA Synthesis kit (Bio-Rad) according to manufacturer’s instruction. cDNAs were subjected to end-point PCR using OneTaq® 2X Master Mix (NEB) or quantitative PCR (qPCR) using iTaq Universal SYBR Green Supermix (Bio-Rad). ..

    Article Title: Field evaluation of the gut microbiome composition of pre-school and school-aged children in Tha Song Yang, Thailand, following oral MDA for STH infections
    Article Snippet: .. Each 96-well PCR plate contained a negative (dH2 O) and positive (ZymoBIOMICS microbial community DNA standard, Zymo Research, Integrated Sciences, Australia) control, with individual reactions conducted under the following conditions: 10 μl OneTaq master mix with standard buffer (2x) (New England Biolabs, USA), 0.4 μl of each primer (10 μM) and 2 μl DNA template made up to a total reaction volume of 20 μl with water were amplified for 3 minutes at 95°C, 45 seconds at 95°C, 60 seconds at 50°C and 90 seconds at 72°C in 20 cycles with a final extension time of 10 minutes at 72°C. ..

    Article Title: Field evaluation of the gut microbiome composition of pre-school and school-aged children in Tha Song Yang, Thailand, following oral MDA for STH infections
    Article Snippet: .. Each PCR reaction was conducted under the following conditions: 15 μl OneTaq master mix with standard buffer (2x) (New England Biolabs, USA), 1.5 μl of each primer (10 μM) and 10 μl of diluted PCR product made up to a total reaction volume of 30 μl using water were amplified for 3 minutes at 95°C, 45 seconds at 95°C, 60 seconds at 55°C and 90 seconds at 72°C in 25 cycles with a final extension time of 10 minutes at 72°C. ..

    Article Title: Application of culture, PCR, and PacBio sequencing for determination of microbial composition of milk from subclinical mastitis dairy cows of smallholder farms
    Article Snippet: .. 2.6 Standard multiplex PCR (mPCR) Standard mPCR was conducted using NEB OneTaq 2× MasterMix with Standard Buffer (10 μL). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Genetic Defects in DNAH2 Underlie Male Infertility With Multiple Morphological Abnormalities of the Sperm Flagella in Humans and Mice
    Article Snippet: .. The cDNAs were applied for PCR using OneTaq® 2X Master Mix (NEB) or quantitative PCR using iTaq Universal SYBR Green Supermix (Bio-Rad). ..

    Article Title: C2cd6-encoded CatSperτ Targets Sperm Calcium Channel to Ca2+ Signaling Domains in the Flagellar Membrane
    Article Snippet: .. 500 ng of the extracted RNA was used for cDNA synthesis using iScriptTM cDNA Synthesis kit (Bio-Rad) according to manufacturer’s instruction. cDNAs were subjected to end-point PCR using OneTaq® 2X Master Mix (NEB) or quantitative PCR (qPCR) using iTaq Universal SYBR Green Supermix (Bio-Rad). ..

    SYBR Green Assay:

    Article Title: Genetic Defects in DNAH2 Underlie Male Infertility With Multiple Morphological Abnormalities of the Sperm Flagella in Humans and Mice
    Article Snippet: .. The cDNAs were applied for PCR using OneTaq® 2X Master Mix (NEB) or quantitative PCR using iTaq Universal SYBR Green Supermix (Bio-Rad). ..

    Article Title: C2cd6-encoded CatSperτ Targets Sperm Calcium Channel to Ca2+ Signaling Domains in the Flagellar Membrane
    Article Snippet: .. 500 ng of the extracted RNA was used for cDNA synthesis using iScriptTM cDNA Synthesis kit (Bio-Rad) according to manufacturer’s instruction. cDNAs were subjected to end-point PCR using OneTaq® 2X Master Mix (NEB) or quantitative PCR (qPCR) using iTaq Universal SYBR Green Supermix (Bio-Rad). ..

    Amplification:

    Article Title: Field evaluation of the gut microbiome composition of pre-school and school-aged children in Tha Song Yang, Thailand, following oral MDA for STH infections
    Article Snippet: .. Each 96-well PCR plate contained a negative (dH2 O) and positive (ZymoBIOMICS microbial community DNA standard, Zymo Research, Integrated Sciences, Australia) control, with individual reactions conducted under the following conditions: 10 μl OneTaq master mix with standard buffer (2x) (New England Biolabs, USA), 0.4 μl of each primer (10 μM) and 2 μl DNA template made up to a total reaction volume of 20 μl with water were amplified for 3 minutes at 95°C, 45 seconds at 95°C, 60 seconds at 50°C and 90 seconds at 72°C in 20 cycles with a final extension time of 10 minutes at 72°C. ..

    Article Title: Antidiabetic activities of Bolanthus spergulifolius (Caryophyllaceae) extracts on insulin-resistant 3T3-L1 adipocytes
    Article Snippet: .. The amplification of cDNA was prepared using the One Taq 2X Master Mix with the Standard Buffer (New England Bio labs Inc., USA) in a final volume of 25 μL using thermal cycler (SureCycler 8800, Agilent Technologies, USA) using primers synthesized by Sentebiolab, Turkey ( ). ..

    Article Title: Field evaluation of the gut microbiome composition of pre-school and school-aged children in Tha Song Yang, Thailand, following oral MDA for STH infections
    Article Snippet: .. Each PCR reaction was conducted under the following conditions: 15 μl OneTaq master mix with standard buffer (2x) (New England Biolabs, USA), 1.5 μl of each primer (10 μM) and 10 μl of diluted PCR product made up to a total reaction volume of 30 μl using water were amplified for 3 minutes at 95°C, 45 seconds at 95°C, 60 seconds at 55°C and 90 seconds at 72°C in 25 cycles with a final extension time of 10 minutes at 72°C. ..

    Synthesized:

    Article Title: Antidiabetic activities of Bolanthus spergulifolius (Caryophyllaceae) extracts on insulin-resistant 3T3-L1 adipocytes
    Article Snippet: .. The amplification of cDNA was prepared using the One Taq 2X Master Mix with the Standard Buffer (New England Bio labs Inc., USA) in a final volume of 25 μL using thermal cycler (SureCycler 8800, Agilent Technologies, USA) using primers synthesized by Sentebiolab, Turkey ( ). ..

    Multiplex Assay:

    Article Title: Application of culture, PCR, and PacBio sequencing for determination of microbial composition of milk from subclinical mastitis dairy cows of smallholder farms
    Article Snippet: .. 2.6 Standard multiplex PCR (mPCR) Standard mPCR was conducted using NEB OneTaq 2× MasterMix with Standard Buffer (10 μL). ..

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  • 98
    New England Biolabs neb onetaq
    Macerprepped DNA is good template for PCR. 1-3, PCR product by <t>NEB</t> Q5® HiFi polymerase. 4-6, PCR product by NEB <t>Onetaq</t> polymerase. I, 4, Miraprepped DNA. 2, 5, Macerprepped DNA with Solution 3 process. 3, 6, Macerprepped DNA with Solution N3 process. M, marker.
    Neb Onetaq, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/neb onetaq/product/New England Biolabs
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    neb onetaq - by Bioz Stars, 2021-09
    98/100 stars
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    95
    New England Biolabs onetaq hot start 2x master mix
    Impact evaluation of Master Mix used in singleplex PCR. In graph: Elizyme = 2X EliZyme HS Robust MIX (Elisabeth Pharmacon, Czech Republic); Accustart II = AccuStart II PCR ToughMix (QuantaBio, Massachusetts, USA); Amplitaq = AmpliTaq Gold 360 Master Mix (Thermo Fisher Scientific, Massachusetts, USA); <t>OneTaq</t> = OneTaq Hot Start <t>2X</t> Master Mix with GC Buffer (New England BioLabs, Massachusetts, USA); Platinum = Platinum Hot Start PCR 2X Master Mix (Invitrogen, California, USA); HotStarTaq = HotStarTaq DNA Polymerase (Qiagen, Germany). YE = Y . enterocolitica; TG = T . gondii ; plus sign in legend = positive sample; minus sign = NTC.
    Onetaq Hot Start 2x Master Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/onetaq hot start 2x master mix/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    onetaq hot start 2x master mix - by Bioz Stars, 2021-09
    95/100 stars
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    96
    New England Biolabs rt pcr master mix
    Pairing of TRAV12-1 + and TRBV2 + CD4 + T cells as determined by emulsion <t>PCR</t> PBMCs were labeled with CFSE, spun, and <t>resuspended</t> in RT-PCR master mix. 300 μl of the oil mixture from the Micellula kit was added on top, and the contents were vortexed for 2.5 minutes. Image was taken at 20× (A). CD4 + T cells were purified from BAL cells or PBMCs from one patient and utilized in emulsion (em) or non-emulsion (non) PCR reactions. A representative 2% agarose gel is shown displaying a DNA ladder (L) and the products obtained after PCR 3 (B). Six DR3 + LS BAL samples were subjected to ePCR, and Vβ usage on TRAV12-1-utilizing BAL CD4 + T cells is shown (C). Vα usage on TRBV2-utilizing BAL CD4 + T cells from six DR3 + LS patients is shown (D). Shown are thirteen αβTCR pairs as determined by ePCR (E). The percentage that the listed TCRα is paired with the listed TCRβ is shown (% α paired with β), as opposed to pairing with other TCRβ in a given patient sample, and vice versa (% β paired with α). Amino acids forming shared or similar CDR3 motifs colored and bolded. TRAV12-1 usage on TRBV2 + cells, TRBV2 usage on TRAV12-1 + cells, TRAV26-1 usage on TRBV20-1 + cells, and TRBV20-1 usage on TRAV26-1 + cells are shown for comparison amongst patient groups (F). Bars represent means ± SD. p values were calculated based on Kruskal-Wallis tests with Dunn’s post-test; * = p
    Rt Pcr Master Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rt pcr master mix/product/New England Biolabs
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rt pcr master mix - by Bioz Stars, 2021-09
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    94
    New England Biolabs onetaq one step rt pcr kit
    qSanger detects SARS-COV-2 RNA when amplified directly from viral particles in transport medium. ( A ) A total of 32 no-template controls, 32 negative control samples (Seracare) and 32 positive samples (Seracare) were assayed. All results were concordant with Seracare and NTC. Three samples were no-calls (undetermined) due to low signal-to-noise ratio in the sequencing results. ( B ) Positive Seracare samples were added to <t>RT-PCR</t> master mix either directly from the VTM or after purification with RNA extraction kit at 125 GCE. The ratio of reference and spike-in intensities were measured by custom data analysis. The mean qSanger ratio was 0.745 (± 0.043 s.e.m., n=8) for direct addition, and 0.97 (± 0.041 s.e.m., n=8) for purified. The coefficient of variation (CV) of positive seracare samples were measured for both Luna and <t>OneTaq</t> polymerase mixes. The CV for Luna direct VTM was 16.4% (n=8), and for Luna purified was 12.1% (n=8). This is consistent with the theoretical counting noise associated with quantifying ~100 molecules.
    Onetaq One Step Rt Pcr Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/onetaq one step rt pcr kit/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    Image Search Results


    Macerprepped DNA is good template for PCR. 1-3, PCR product by NEB Q5® HiFi polymerase. 4-6, PCR product by NEB Onetaq polymerase. I, 4, Miraprepped DNA. 2, 5, Macerprepped DNA with Solution 3 process. 3, 6, Macerprepped DNA with Solution N3 process. M, marker.

    Journal: bioRxiv

    Article Title: The Macerprep: a minimalist kit- and enzyme-free high-yield miniprep utilising alkaline lysis and alkaline hydrolysis principles

    doi: 10.1101/2020.08.13.249607

    Figure Lengend Snippet: Macerprepped DNA is good template for PCR. 1-3, PCR product by NEB Q5® HiFi polymerase. 4-6, PCR product by NEB Onetaq polymerase. I, 4, Miraprepped DNA. 2, 5, Macerprepped DNA with Solution 3 process. 3, 6, Macerprepped DNA with Solution N3 process. M, marker.

    Article Snippet: According to the NEB calculator (NEB, online tool), Tm for NEB Q5 and NEB Onetaq was determined to be 52°C and 61°C.

    Techniques: Polymerase Chain Reaction, Marker

    Impact evaluation of Master Mix used in singleplex PCR. In graph: Elizyme = 2X EliZyme HS Robust MIX (Elisabeth Pharmacon, Czech Republic); Accustart II = AccuStart II PCR ToughMix (QuantaBio, Massachusetts, USA); Amplitaq = AmpliTaq Gold 360 Master Mix (Thermo Fisher Scientific, Massachusetts, USA); OneTaq = OneTaq Hot Start 2X Master Mix with GC Buffer (New England BioLabs, Massachusetts, USA); Platinum = Platinum Hot Start PCR 2X Master Mix (Invitrogen, California, USA); HotStarTaq = HotStarTaq DNA Polymerase (Qiagen, Germany). YE = Y . enterocolitica; TG = T . gondii ; plus sign in legend = positive sample; minus sign = NTC.

    Journal: Scientific Reports

    Article Title: A novel perspective on MOL-PCR optimization and MAGPIX analysis of in-house multiplex foodborne pathogens detection assay

    doi: 10.1038/s41598-019-40035-5

    Figure Lengend Snippet: Impact evaluation of Master Mix used in singleplex PCR. In graph: Elizyme = 2X EliZyme HS Robust MIX (Elisabeth Pharmacon, Czech Republic); Accustart II = AccuStart II PCR ToughMix (QuantaBio, Massachusetts, USA); Amplitaq = AmpliTaq Gold 360 Master Mix (Thermo Fisher Scientific, Massachusetts, USA); OneTaq = OneTaq Hot Start 2X Master Mix with GC Buffer (New England BioLabs, Massachusetts, USA); Platinum = Platinum Hot Start PCR 2X Master Mix (Invitrogen, California, USA); HotStarTaq = HotStarTaq DNA Polymerase (Qiagen, Germany). YE = Y . enterocolitica; TG = T . gondii ; plus sign in legend = positive sample; minus sign = NTC.

    Article Snippet: Several master mixes were tested in the optimization experiments, namely AccuStart II PCR ToughMix (QuantaBio, Massachusetts, USA), AmpliTaq Gold 360 Master Mix (Thermo Fisher Scientific, Massachusetts, USA), OneTaq Hot Start 2X Master Mix with GC Buffer (New England BioLabs, Massachusetts, USA), Platinum Hot Start PCR Master Mix (Invitrogen, California, USA), and HotStarTaq DNA Polymerase (Qiagen, Germany).

    Techniques: Polymerase Chain Reaction, Hot Start PCR

    Pairing of TRAV12-1 + and TRBV2 + CD4 + T cells as determined by emulsion PCR PBMCs were labeled with CFSE, spun, and resuspended in RT-PCR master mix. 300 μl of the oil mixture from the Micellula kit was added on top, and the contents were vortexed for 2.5 minutes. Image was taken at 20× (A). CD4 + T cells were purified from BAL cells or PBMCs from one patient and utilized in emulsion (em) or non-emulsion (non) PCR reactions. A representative 2% agarose gel is shown displaying a DNA ladder (L) and the products obtained after PCR 3 (B). Six DR3 + LS BAL samples were subjected to ePCR, and Vβ usage on TRAV12-1-utilizing BAL CD4 + T cells is shown (C). Vα usage on TRBV2-utilizing BAL CD4 + T cells from six DR3 + LS patients is shown (D). Shown are thirteen αβTCR pairs as determined by ePCR (E). The percentage that the listed TCRα is paired with the listed TCRβ is shown (% α paired with β), as opposed to pairing with other TCRβ in a given patient sample, and vice versa (% β paired with α). Amino acids forming shared or similar CDR3 motifs colored and bolded. TRAV12-1 usage on TRBV2 + cells, TRBV2 usage on TRAV12-1 + cells, TRAV26-1 usage on TRBV20-1 + cells, and TRBV20-1 usage on TRAV26-1 + cells are shown for comparison amongst patient groups (F). Bars represent means ± SD. p values were calculated based on Kruskal-Wallis tests with Dunn’s post-test; * = p

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Shared αβ T Cell Receptor Usage in Lungs of Sarcoidosis Patients with Löfgren’s Syndrome

    doi: 10.4049/jimmunol.1700570

    Figure Lengend Snippet: Pairing of TRAV12-1 + and TRBV2 + CD4 + T cells as determined by emulsion PCR PBMCs were labeled with CFSE, spun, and resuspended in RT-PCR master mix. 300 μl of the oil mixture from the Micellula kit was added on top, and the contents were vortexed for 2.5 minutes. Image was taken at 20× (A). CD4 + T cells were purified from BAL cells or PBMCs from one patient and utilized in emulsion (em) or non-emulsion (non) PCR reactions. A representative 2% agarose gel is shown displaying a DNA ladder (L) and the products obtained after PCR 3 (B). Six DR3 + LS BAL samples were subjected to ePCR, and Vβ usage on TRAV12-1-utilizing BAL CD4 + T cells is shown (C). Vα usage on TRBV2-utilizing BAL CD4 + T cells from six DR3 + LS patients is shown (D). Shown are thirteen αβTCR pairs as determined by ePCR (E). The percentage that the listed TCRα is paired with the listed TCRβ is shown (% α paired with β), as opposed to pairing with other TCRβ in a given patient sample, and vice versa (% β paired with α). Amino acids forming shared or similar CDR3 motifs colored and bolded. TRAV12-1 usage on TRBV2 + cells, TRBV2 usage on TRAV12-1 + cells, TRAV26-1 usage on TRBV20-1 + cells, and TRBV20-1 usage on TRAV26-1 + cells are shown for comparison amongst patient groups (F). Bars represent means ± SD. p values were calculated based on Kruskal-Wallis tests with Dunn’s post-test; * = p

    Article Snippet: Cells were centrifuged and resuspended in 50 μl of RT-PCR master mix [1x OneTaq One-Step RT-PCR buffer and enzyme (New England Biolabs, Ipswich, MA), 10 U RNase out (Invitrogen, Carlsbad, CA), 2.5 mg/ml acetylated BSA (Sigma-Aldrich, St. Louis, MO), 400 nM each of the C region primers and Stepout primers, and 40 nM each of the V region primers as described in ].

    Techniques: Polymerase Chain Reaction, Labeling, Reverse Transcription Polymerase Chain Reaction, Purification, Agarose Gel Electrophoresis

    qSanger detects SARS-COV-2 RNA when amplified directly from viral particles in transport medium. ( A ) A total of 32 no-template controls, 32 negative control samples (Seracare) and 32 positive samples (Seracare) were assayed. All results were concordant with Seracare and NTC. Three samples were no-calls (undetermined) due to low signal-to-noise ratio in the sequencing results. ( B ) Positive Seracare samples were added to RT-PCR master mix either directly from the VTM or after purification with RNA extraction kit at 125 GCE. The ratio of reference and spike-in intensities were measured by custom data analysis. The mean qSanger ratio was 0.745 (± 0.043 s.e.m., n=8) for direct addition, and 0.97 (± 0.041 s.e.m., n=8) for purified. The coefficient of variation (CV) of positive seracare samples were measured for both Luna and OneTaq polymerase mixes. The CV for Luna direct VTM was 16.4% (n=8), and for Luna purified was 12.1% (n=8). This is consistent with the theoretical counting noise associated with quantifying ~100 molecules.

    Journal: bioRxiv

    Article Title: A Highly Scalable and Rapidly Deployable RNA Extraction-Free COVID-19 Assay by Quantitative Sanger Sequencing

    doi: 10.1101/2020.04.07.029199

    Figure Lengend Snippet: qSanger detects SARS-COV-2 RNA when amplified directly from viral particles in transport medium. ( A ) A total of 32 no-template controls, 32 negative control samples (Seracare) and 32 positive samples (Seracare) were assayed. All results were concordant with Seracare and NTC. Three samples were no-calls (undetermined) due to low signal-to-noise ratio in the sequencing results. ( B ) Positive Seracare samples were added to RT-PCR master mix either directly from the VTM or after purification with RNA extraction kit at 125 GCE. The ratio of reference and spike-in intensities were measured by custom data analysis. The mean qSanger ratio was 0.745 (± 0.043 s.e.m., n=8) for direct addition, and 0.97 (± 0.041 s.e.m., n=8) for purified. The coefficient of variation (CV) of positive seracare samples were measured for both Luna and OneTaq polymerase mixes. The CV for Luna direct VTM was 16.4% (n=8), and for Luna purified was 12.1% (n=8). This is consistent with the theoretical counting noise associated with quantifying ~100 molecules.

    Article Snippet: qSanger Amplification Reverse transcription and amplification for was performed using OneTaq One-Step RT-PCR Kit from NEB (cat. # E5315S).

    Techniques: Amplification, Negative Control, Sequencing, Reverse Transcription Polymerase Chain Reaction, Purification, RNA Extraction