onetaq  (New England Biolabs)


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  • 94
    Name:
    OneTaq RT PCR Kit
    Description:
    OneTaq RT PCR Kit 30 rxns
    Catalog Number:
    e5310s
    Price:
    146
    Category:
    PCR related Kits
    Size:
    30 rxns
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    Structured Review

    New England Biolabs onetaq
    OneTaq RT PCR Kit
    OneTaq RT PCR Kit 30 rxns
    https://www.bioz.com/result/onetaq/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    onetaq - by Bioz Stars, 2021-03
    94/100 stars

    Images

    Related Articles

    Clone Assay:

    Article Title: Siglec-15 as an immune suppressor and potential target for normalization cancer immunotherapy
    Article Snippet: Commercial antibodies used in flow cytometry and in vitro cultures were purchased from BD Biosciences (San Jose, CA), BioLegend (San Diego, CA), Thermo Fisher Scientific or R & D systems (Minneapolis, MN). .. For gene cloning of mouse and human Siglec-15, cDNAs were prepared from mouse BMDMs and BMDCs and human macrophages and genes were obtained by RT-PCR using RNeasy Mini Kit (Qiagen, Hilden, Germany) and reverse transcription Kit (Thermo Fisher Scientific) or One Taq RT-PCR Kit (New England Biolabs, Ipswich, MA). .. For analysis of murine Siglec-15 gene expression, cDNA was determined by quantitative PCR using the following primers: forward 5’-CAGCACCGAGATGTTGACGA-3’; reverse 5’-ACGATCGCTATGAGAGTCGC-3’.

    Article Title: The Adc/Lmb System Mediates Zinc Acquisition in Streptococcus agalactiae and Contributes to Bacterial Growth and Survival
    Article Snippet: Oligonucleotides (Eurogentec and Sigma-Aldrich) used in this study are listed in . .. Analytical PCR used standard OneTaq polymerase (New England BioLabs [NEB]) and PCR for cloning or sequencing was carried out with Q5 high-fidelity DNA polymerase (NEB). .. The resulting PCR fragments were purified with a NucleoSpin gel and PCR cleanup kit (Macherey-Nagel), according to the manufacturer's instructions.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Siglec-15 as an immune suppressor and potential target for normalization cancer immunotherapy
    Article Snippet: Commercial antibodies used in flow cytometry and in vitro cultures were purchased from BD Biosciences (San Jose, CA), BioLegend (San Diego, CA), Thermo Fisher Scientific or R & D systems (Minneapolis, MN). .. For gene cloning of mouse and human Siglec-15, cDNAs were prepared from mouse BMDMs and BMDCs and human macrophages and genes were obtained by RT-PCR using RNeasy Mini Kit (Qiagen, Hilden, Germany) and reverse transcription Kit (Thermo Fisher Scientific) or One Taq RT-PCR Kit (New England Biolabs, Ipswich, MA). .. For analysis of murine Siglec-15 gene expression, cDNA was determined by quantitative PCR using the following primers: forward 5’-CAGCACCGAGATGTTGACGA-3’; reverse 5’-ACGATCGCTATGAGAGTCGC-3’.

    Article Title: Transcriptional elongation requires DNA break-induced signalling
    Article Snippet: RT–qPCR HeLa cells were starved in 0.1% serum for 17.5 h and then stimulated with 18% serum for indicated time durations. .. Total RNA samples were collected and reverse transcribed into complementary DNA using OneTaq RT-PCR Kit (New England Biolabs). .. Real-time PCR was performed in CFX96 Real-time PCR detection system (Bio-Rad) using β-actin as an internal control.

    Amplification:

    Article Title: Crystal structure of Bacillus anthracis virulence regulator AtxA and effects of phosphorylated histidines on multimerization and activity
    Article Snippet: E. coli strain GM2163 ( dam dcm mutant) was used to generate unmethylated plasmid DNA for electroporation of B. anthracis ( ). .. DNA was amplified by the PCR using KOD (Novagen, Madison, WI), Onetaq, or Phusion polymerase (New England Biolabs, Ipswich, MA). ..

    Polymerase Chain Reaction:

    Article Title: Crystal structure of Bacillus anthracis virulence regulator AtxA and effects of phosphorylated histidines on multimerization and activity
    Article Snippet: E. coli strain GM2163 ( dam dcm mutant) was used to generate unmethylated plasmid DNA for electroporation of B. anthracis ( ). .. DNA was amplified by the PCR using KOD (Novagen, Madison, WI), Onetaq, or Phusion polymerase (New England Biolabs, Ipswich, MA). ..

    Article Title: The Adc/Lmb System Mediates Zinc Acquisition in Streptococcus agalactiae and Contributes to Bacterial Growth and Survival
    Article Snippet: Oligonucleotides (Eurogentec and Sigma-Aldrich) used in this study are listed in . .. Analytical PCR used standard OneTaq polymerase (New England BioLabs [NEB]) and PCR for cloning or sequencing was carried out with Q5 high-fidelity DNA polymerase (NEB). .. The resulting PCR fragments were purified with a NucleoSpin gel and PCR cleanup kit (Macherey-Nagel), according to the manufacturer's instructions.

    Article Title: Characterization of evolutionarily conserved key players affecting eukaryotic flagellar motility and fertility using a moss model
    Article Snippet: Single genomic locus binding properties were tested by using BLAST against the V3.3 genome of P. patens. .. Real-time PCR was performed using 5 ng of cDNA as input for PCR reaction with OneTaq from New England Biolabs). .. PCR products were visualized via gel electrophoresis using peqGREEN from (VWR, Germany).

    Sequencing:

    Article Title: The Adc/Lmb System Mediates Zinc Acquisition in Streptococcus agalactiae and Contributes to Bacterial Growth and Survival
    Article Snippet: Oligonucleotides (Eurogentec and Sigma-Aldrich) used in this study are listed in . .. Analytical PCR used standard OneTaq polymerase (New England BioLabs [NEB]) and PCR for cloning or sequencing was carried out with Q5 high-fidelity DNA polymerase (NEB). .. The resulting PCR fragments were purified with a NucleoSpin gel and PCR cleanup kit (Macherey-Nagel), according to the manufacturer's instructions.

    Real-time Polymerase Chain Reaction:

    Article Title: Characterization of evolutionarily conserved key players affecting eukaryotic flagellar motility and fertility using a moss model
    Article Snippet: Single genomic locus binding properties were tested by using BLAST against the V3.3 genome of P. patens. .. Real-time PCR was performed using 5 ng of cDNA as input for PCR reaction with OneTaq from New England Biolabs). .. PCR products were visualized via gel electrophoresis using peqGREEN from (VWR, Germany).

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  • 95
    New England Biolabs rt pcr master mix
    Pairing of TRAV12-1 + and TRBV2 + CD4 + T cells as determined by emulsion <t>PCR</t> PBMCs were labeled with CFSE, spun, and <t>resuspended</t> in RT-PCR master mix. 300 μl of the oil mixture from the Micellula kit was added on top, and the contents were vortexed for 2.5 minutes. Image was taken at 20× (A). CD4 + T cells were purified from BAL cells or PBMCs from one patient and utilized in emulsion (em) or non-emulsion (non) PCR reactions. A representative 2% agarose gel is shown displaying a DNA ladder (L) and the products obtained after PCR 3 (B). Six DR3 + LS BAL samples were subjected to ePCR, and Vβ usage on TRAV12-1-utilizing BAL CD4 + T cells is shown (C). Vα usage on TRBV2-utilizing BAL CD4 + T cells from six DR3 + LS patients is shown (D). Shown are thirteen αβTCR pairs as determined by ePCR (E). The percentage that the listed TCRα is paired with the listed TCRβ is shown (% α paired with β), as opposed to pairing with other TCRβ in a given patient sample, and vice versa (% β paired with α). Amino acids forming shared or similar CDR3 motifs colored and bolded. TRAV12-1 usage on TRBV2 + cells, TRBV2 usage on TRAV12-1 + cells, TRAV26-1 usage on TRBV20-1 + cells, and TRBV20-1 usage on TRAV26-1 + cells are shown for comparison amongst patient groups (F). Bars represent means ± SD. p values were calculated based on Kruskal-Wallis tests with Dunn’s post-test; * = p
    Rt Pcr Master Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rt pcr master mix/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rt pcr master mix - by Bioz Stars, 2021-03
    95/100 stars
      Buy from Supplier

    94
    New England Biolabs onetaq one step rt pcr kit
    qSanger detects SARS-COV-2 RNA when amplified directly from viral particles in transport medium. ( A ) A total of 32 no-template controls, 32 negative control samples (Seracare) and 32 positive samples (Seracare) were assayed. All results were concordant with Seracare and NTC. Three samples were no-calls (undetermined) due to low signal-to-noise ratio in the sequencing results. ( B ) Positive Seracare samples were added to <t>RT-PCR</t> master mix either directly from the VTM or after purification with RNA extraction kit at 125 GCE. The ratio of reference and spike-in intensities were measured by custom data analysis. The mean qSanger ratio was 0.745 (± 0.043 s.e.m., n=8) for direct addition, and 0.97 (± 0.041 s.e.m., n=8) for purified. The coefficient of variation (CV) of positive seracare samples were measured for both Luna and <t>OneTaq</t> polymerase mixes. The CV for Luna direct VTM was 16.4% (n=8), and for Luna purified was 12.1% (n=8). This is consistent with the theoretical counting noise associated with quantifying ~100 molecules.
    Onetaq One Step Rt Pcr Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/onetaq one step rt pcr kit/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    onetaq one step rt pcr kit - by Bioz Stars, 2021-03
    94/100 stars
      Buy from Supplier

    93
    New England Biolabs onetaq hot start 2x master mix
    Impact evaluation of Master Mix used in singleplex PCR. In graph: Elizyme = 2X EliZyme HS Robust MIX (Elisabeth Pharmacon, Czech Republic); Accustart II = AccuStart II PCR ToughMix (QuantaBio, Massachusetts, USA); Amplitaq = AmpliTaq Gold 360 Master Mix (Thermo Fisher Scientific, Massachusetts, USA); <t>OneTaq</t> = OneTaq Hot Start <t>2X</t> Master Mix with GC Buffer (New England BioLabs, Massachusetts, USA); Platinum = Platinum Hot Start PCR 2X Master Mix (Invitrogen, California, USA); HotStarTaq = HotStarTaq DNA Polymerase (Qiagen, Germany). YE = Y . enterocolitica; TG = T . gondii ; plus sign in legend = positive sample; minus sign = NTC.
    Onetaq Hot Start 2x Master Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/onetaq hot start 2x master mix/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    onetaq hot start 2x master mix - by Bioz Stars, 2021-03
    93/100 stars
      Buy from Supplier

    Image Search Results


    Pairing of TRAV12-1 + and TRBV2 + CD4 + T cells as determined by emulsion PCR PBMCs were labeled with CFSE, spun, and resuspended in RT-PCR master mix. 300 μl of the oil mixture from the Micellula kit was added on top, and the contents were vortexed for 2.5 minutes. Image was taken at 20× (A). CD4 + T cells were purified from BAL cells or PBMCs from one patient and utilized in emulsion (em) or non-emulsion (non) PCR reactions. A representative 2% agarose gel is shown displaying a DNA ladder (L) and the products obtained after PCR 3 (B). Six DR3 + LS BAL samples were subjected to ePCR, and Vβ usage on TRAV12-1-utilizing BAL CD4 + T cells is shown (C). Vα usage on TRBV2-utilizing BAL CD4 + T cells from six DR3 + LS patients is shown (D). Shown are thirteen αβTCR pairs as determined by ePCR (E). The percentage that the listed TCRα is paired with the listed TCRβ is shown (% α paired with β), as opposed to pairing with other TCRβ in a given patient sample, and vice versa (% β paired with α). Amino acids forming shared or similar CDR3 motifs colored and bolded. TRAV12-1 usage on TRBV2 + cells, TRBV2 usage on TRAV12-1 + cells, TRAV26-1 usage on TRBV20-1 + cells, and TRBV20-1 usage on TRAV26-1 + cells are shown for comparison amongst patient groups (F). Bars represent means ± SD. p values were calculated based on Kruskal-Wallis tests with Dunn’s post-test; * = p

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Shared αβ T Cell Receptor Usage in Lungs of Sarcoidosis Patients with Löfgren’s Syndrome

    doi: 10.4049/jimmunol.1700570

    Figure Lengend Snippet: Pairing of TRAV12-1 + and TRBV2 + CD4 + T cells as determined by emulsion PCR PBMCs were labeled with CFSE, spun, and resuspended in RT-PCR master mix. 300 μl of the oil mixture from the Micellula kit was added on top, and the contents were vortexed for 2.5 minutes. Image was taken at 20× (A). CD4 + T cells were purified from BAL cells or PBMCs from one patient and utilized in emulsion (em) or non-emulsion (non) PCR reactions. A representative 2% agarose gel is shown displaying a DNA ladder (L) and the products obtained after PCR 3 (B). Six DR3 + LS BAL samples were subjected to ePCR, and Vβ usage on TRAV12-1-utilizing BAL CD4 + T cells is shown (C). Vα usage on TRBV2-utilizing BAL CD4 + T cells from six DR3 + LS patients is shown (D). Shown are thirteen αβTCR pairs as determined by ePCR (E). The percentage that the listed TCRα is paired with the listed TCRβ is shown (% α paired with β), as opposed to pairing with other TCRβ in a given patient sample, and vice versa (% β paired with α). Amino acids forming shared or similar CDR3 motifs colored and bolded. TRAV12-1 usage on TRBV2 + cells, TRBV2 usage on TRAV12-1 + cells, TRAV26-1 usage on TRBV20-1 + cells, and TRBV20-1 usage on TRAV26-1 + cells are shown for comparison amongst patient groups (F). Bars represent means ± SD. p values were calculated based on Kruskal-Wallis tests with Dunn’s post-test; * = p

    Article Snippet: Cells were centrifuged and resuspended in 50 μl of RT-PCR master mix [1x OneTaq One-Step RT-PCR buffer and enzyme (New England Biolabs, Ipswich, MA), 10 U RNase out (Invitrogen, Carlsbad, CA), 2.5 mg/ml acetylated BSA (Sigma-Aldrich, St. Louis, MO), 400 nM each of the C region primers and Stepout primers, and 40 nM each of the V region primers as described in ].

    Techniques: Polymerase Chain Reaction, Labeling, Reverse Transcription Polymerase Chain Reaction, Purification, Agarose Gel Electrophoresis

    Macerprepped DNA is good template for PCR. 1-3, PCR product by NEB Q5® HiFi polymerase. 4-6, PCR product by NEB Onetaq polymerase. I, 4, Miraprepped DNA. 2, 5, Macerprepped DNA with Solution 3 process. 3, 6, Macerprepped DNA with Solution N3 process. M, marker.

    Journal: bioRxiv

    Article Title: The Macerprep: a minimalist kit- and enzyme-free high-yield miniprep utilising alkaline lysis and alkaline hydrolysis principles

    doi: 10.1101/2020.08.13.249607

    Figure Lengend Snippet: Macerprepped DNA is good template for PCR. 1-3, PCR product by NEB Q5® HiFi polymerase. 4-6, PCR product by NEB Onetaq polymerase. I, 4, Miraprepped DNA. 2, 5, Macerprepped DNA with Solution 3 process. 3, 6, Macerprepped DNA with Solution N3 process. M, marker.

    Article Snippet: According to the NEB calculator (NEB, online tool), Tm for NEB Q5 and NEB Onetaq was determined to be 52°C and 61°C.

    Techniques: Polymerase Chain Reaction, Marker

    qSanger detects SARS-COV-2 RNA when amplified directly from viral particles in transport medium. ( A ) A total of 32 no-template controls, 32 negative control samples (Seracare) and 32 positive samples (Seracare) were assayed. All results were concordant with Seracare and NTC. Three samples were no-calls (undetermined) due to low signal-to-noise ratio in the sequencing results. ( B ) Positive Seracare samples were added to RT-PCR master mix either directly from the VTM or after purification with RNA extraction kit at 125 GCE. The ratio of reference and spike-in intensities were measured by custom data analysis. The mean qSanger ratio was 0.745 (± 0.043 s.e.m., n=8) for direct addition, and 0.97 (± 0.041 s.e.m., n=8) for purified. The coefficient of variation (CV) of positive seracare samples were measured for both Luna and OneTaq polymerase mixes. The CV for Luna direct VTM was 16.4% (n=8), and for Luna purified was 12.1% (n=8). This is consistent with the theoretical counting noise associated with quantifying ~100 molecules.

    Journal: bioRxiv

    Article Title: A Highly Scalable and Rapidly Deployable RNA Extraction-Free COVID-19 Assay by Quantitative Sanger Sequencing

    doi: 10.1101/2020.04.07.029199

    Figure Lengend Snippet: qSanger detects SARS-COV-2 RNA when amplified directly from viral particles in transport medium. ( A ) A total of 32 no-template controls, 32 negative control samples (Seracare) and 32 positive samples (Seracare) were assayed. All results were concordant with Seracare and NTC. Three samples were no-calls (undetermined) due to low signal-to-noise ratio in the sequencing results. ( B ) Positive Seracare samples were added to RT-PCR master mix either directly from the VTM or after purification with RNA extraction kit at 125 GCE. The ratio of reference and spike-in intensities were measured by custom data analysis. The mean qSanger ratio was 0.745 (± 0.043 s.e.m., n=8) for direct addition, and 0.97 (± 0.041 s.e.m., n=8) for purified. The coefficient of variation (CV) of positive seracare samples were measured for both Luna and OneTaq polymerase mixes. The CV for Luna direct VTM was 16.4% (n=8), and for Luna purified was 12.1% (n=8). This is consistent with the theoretical counting noise associated with quantifying ~100 molecules.

    Article Snippet: qSanger Amplification Reverse transcription and amplification for was performed using OneTaq One-Step RT-PCR Kit from NEB (cat. # E5315S).

    Techniques: Amplification, Negative Control, Sequencing, Reverse Transcription Polymerase Chain Reaction, Purification, RNA Extraction

    Impact evaluation of Master Mix used in singleplex PCR. In graph: Elizyme = 2X EliZyme HS Robust MIX (Elisabeth Pharmacon, Czech Republic); Accustart II = AccuStart II PCR ToughMix (QuantaBio, Massachusetts, USA); Amplitaq = AmpliTaq Gold 360 Master Mix (Thermo Fisher Scientific, Massachusetts, USA); OneTaq = OneTaq Hot Start 2X Master Mix with GC Buffer (New England BioLabs, Massachusetts, USA); Platinum = Platinum Hot Start PCR 2X Master Mix (Invitrogen, California, USA); HotStarTaq = HotStarTaq DNA Polymerase (Qiagen, Germany). YE = Y . enterocolitica; TG = T . gondii ; plus sign in legend = positive sample; minus sign = NTC.

    Journal: Scientific Reports

    Article Title: A novel perspective on MOL-PCR optimization and MAGPIX analysis of in-house multiplex foodborne pathogens detection assay

    doi: 10.1038/s41598-019-40035-5

    Figure Lengend Snippet: Impact evaluation of Master Mix used in singleplex PCR. In graph: Elizyme = 2X EliZyme HS Robust MIX (Elisabeth Pharmacon, Czech Republic); Accustart II = AccuStart II PCR ToughMix (QuantaBio, Massachusetts, USA); Amplitaq = AmpliTaq Gold 360 Master Mix (Thermo Fisher Scientific, Massachusetts, USA); OneTaq = OneTaq Hot Start 2X Master Mix with GC Buffer (New England BioLabs, Massachusetts, USA); Platinum = Platinum Hot Start PCR 2X Master Mix (Invitrogen, California, USA); HotStarTaq = HotStarTaq DNA Polymerase (Qiagen, Germany). YE = Y . enterocolitica; TG = T . gondii ; plus sign in legend = positive sample; minus sign = NTC.

    Article Snippet: Several master mixes were tested in the optimization experiments, namely AccuStart II PCR ToughMix (QuantaBio, Massachusetts, USA), AmpliTaq Gold 360 Master Mix (Thermo Fisher Scientific, Massachusetts, USA), OneTaq Hot Start 2X Master Mix with GC Buffer (New England BioLabs, Massachusetts, USA), Platinum Hot Start PCR Master Mix (Invitrogen, California, USA), and HotStarTaq DNA Polymerase (Qiagen, Germany).

    Techniques: Polymerase Chain Reaction, Hot Start PCR