Okadaic Acid, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Activation of ?-opioid receptors inhibits calcium-currents in the vestibular afferent neurons of the rat through a cAMP dependent mechanism"
Article Title: Activation of ?-opioid receptors inhibits calcium-currents in the vestibular afferent neurons of the rat through a cAMP dependent mechanism
Journal: Frontiers in Cellular Neuroscience
Figure Legend Snippet: Inhibition of the I Ca by MOR activation was reverted by 8-Br-cAMP or IBMX and mimicked by H89. (A) Time course of the I Ca amplitude under the control condition, after 1 μM DAMGO and DAMGO plus 1 mM 8-Br-cAMP application. The application of 8-Br-cAMP reverted the DAMGO inhibition 92 ± 7% ( n = 7). (B) Traces of the I Ca under the control condition, after DAMGO and after the co-application of DAMGO and 8-Br-cAMP. (C) Time course of the I Ca peak amplitude under the control condition, after the use of 1 μM H89 and after 1 μM H89 plus 1 μM DAMGO perfusion. The H89 mimicked the inhibitory effect of DAMGO; the co-application of H89 and DAMGO did not add to its inhibitory effects ( n = 9). (D) I-V relationship of calcium current showed that the phosphatase inhibition with 100 nM okadaic acid enhanced the HVA and LVA currents, shifted the peak of the IV relationship to less depolarized values, but was unable to occlude the inhibitory effect of 1 μM DAMGO. (E) Representative traces of the LVA and HVA currents in control, under perfusion with 100 nM okadaic acid and coapplication of 100 nM okadaic acid and 1 μM DAMGO. The okadaic acid enhanced the I Ca but was unable to occlude the DAMGO effect. The line dotted line represents zero current. The voltage clamp protocol is shown below the recordings. (F) Bar graph summarizing the effects produced by the various drugs used to discern the pathways involved in MOR actions upon both the LVA and HVA current components ( * means P
Techniques Used: Inhibition, Activation Assay, Produced
Figure Legend Snippet: MOR activation pathway leading to I Ca inhibition in the VANs. The use of PTX showed that the I Ca inhibition by MOR activation is Gα i/o dependent. Use of paired pulses showed that the VD signaling mechanism does not significantly participate in the I Ca inhibition. The use of forskolin, 8-Br-cAMP, IBMX, H89, and okadaic acid indicate that the I Ca inhibition (LVA and HVA) by MOR activation is mediated by the cAMP pathway. Involving the Gα i/o inhibition, AC inhibition, decreased of cAMP levels, decreased of PKA activity and decreased of PKA-dependent phosphorylation of calcium channels. In the scheme, arrow-endings indicate activation and the line-endings indicate inhibition. The names and lines in red indicate the drugs used. AC, Adenylyl cyclase; GP, G Protein; PDE, Phosphodiesterase; VD, Voltage dependent signaling mechanism; VGCC, Voltage gated calcium channels; VI, Voltage independent signaling mechanism.
Techniques Used: Activation Assay, Inhibition, Activity Assay